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Efektivitas Gen CP PSTV dalam Memproteksi Nicotiana benthamiana Transgenik TO terhadap Serangan Peanut Stripe Virus Sholeh Avivi; Sudarsono Sudarsono; Satriyas Ilyas; Hajrial Aswidinnoor
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12103

Abstract

The aims of this research were: (1) to obtain the transgenic Nicotiana benthamiana T0 which have various cp Peanut stripe virus (PStV) genes construct (pBINRCP 1, pBINRCP2, pBINRCP3, and pBINRCP4); (2) to investigate the resistance of transgenic N. benthamianaT0 toward PStV infection; (3) to investigate the effectiveness of those construct to protect N. benthamiana T0 toward PStV infection. To achieve those objectives N. benthamiana T0 (70 plants) were regenerated, PCR tested and infected with PStV using biological analysis methods. The result showed that all of PStV construct gave the resistancy toward PStV infection. The phenotypic respon of those plants were the PBINRCPl plants 35.7% resistance, 0% recovery, and 64.3% susceptible, the PBINRCP2 plants 41.7%, resistance, 0% recovery, and 58,3% susceptible, the PBINRCP3 plants 71.4% resistance, 0% recovery, and 28.6% susceptible, and the PBINRCP4 plants 25.0% resistance, 25.0% recovery, and 50.0% susceptible.
MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERS OF CASSAVA (MANIHOT ESCULENTA CRANTZ) WHICH WET TOLERANT Rahmawati Rahmawati; Tri Agus Siswoyo; Didik Puji Restanto; Sri Hartatik; Sigit Soeparjono; Sholeh Avivi
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Weather conditions often unstable changed and increase the floods of agricultural land. Land which was originally drought will experience wetness and need variety of a plant that wetness tolerant. This study were aimed to get cassava that wet tolerant and to study the morphological, physiological characters of cassava grown on wetness land. The experiment was conducted based on factorial random plot design that consisting two factors with five replications. The first factor were four varieties namely: V1 = Daun Ganja; V2 = Sawi Ketan; V3 = Kasesat Beracun; V4 = Gajah. The second factor was wet treatment consisting of 2 the condition that were K0 = as control, media with 100% field capacity; K1 = as wet treatment by watering of media maintain on -10 cm from media surface. The difference between treatments tested with Duncan Multiple Range Test (DMRT) with the probability 5%. Wet treatment started when plant aged was 2 months after planting, during eight weeks. The observations on variables based on morphological and physiological characters. Result showed that every varieties give the different response on wet treatment. Best response on wet tolerant variety shown by variety code of V2 (Sawi Ketan) were indicated by plant height, total leaf number produced, stem diameter, fresh weight of root, dry weight of root, fresh weight of shoot, dry weight of shoot, photosynthesis index, stomata conductivity and root volume.
The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment Sholeh Avivi; Ralf G. Dietzgen; Colleen M. Higgins; Sudarsono .
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

The aims of this research were to (1) compare the effectiveness of established Agro bacterium mediated transformation and bombardment mediated transformation on peanut (2) evaluate the integration of green fluorescent protein (GFP) and hygromycin resistance (hygr) gene on the peanut embryosomatics. To achieve those objectives we shoot 1200 explants using bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both of Agrobacterium mediated transformation and particle bombardment could give positive embryos transformed.  Particle bombardment gave 4.5% transformation efficiency while Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that particle bombardment more efficient and gave more transgenic explants than Agrobacterium mediated transformation. Keywords: agrobacterium, bombardment, GFP.
PENGARUH PERLAKUAN SORTASI, NATRIUM HIPOKLORIT DAN FUNGISIDA PADA KACANG TANAH UNTUK MENGELIMINASI KONTAMINASI ASPERGILLUS FLAVUS Sholeh Avivi .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 5 No. 1 (2005): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.656 KB) | DOI: 10.23960/j.hptt.1558-65

Abstract

The Effect of Sorting, Sodium hypochloride (NaClO), and Fungicide Treatment on Peanut in Elimination the Aspergillus flavus Contamination. The humid tropical condition of Indonesia, the post harvest treatment, and the storage practices favour rapid growth and wide spread Aspergillus infestation on peanut. The objectives of this research was to find the best technique in controlling the Aspergillus infection on peanut. To achieve those objectives, complete randomised design in factorial with three factors was applied. The factors were grading and colour sorting (A1 = grading and colour sorting; A2 = without grading and colour sorting), NaClO blanching (B1 = without blanching; B2 = 1.25% NaClO blanching; B3=2.5% NaClO blanching), and Benlate T-20 WP treatment (C1 = 2,5 g/L; C2 = 3 g/L; C3 = 3,5 g/L). The result showed that the best combination treatment was A2B2C3 with the minimum amount of spore on peanut seed. With those combination treatment we concluded that the grading and colour sorting, the 1.25% NaClO blanching, and the application of 3,5 g/L Benlate T-20 WP could reduced the Aspergillus contamination up to 80% compare with A1B1C3 treatment.
EFEK BAKTERI PELARUT FOSFAT TERHADAP PERTUMBUHAN ASPERGILLUS FLAVUS PADA PERKECAMBAHAN KACANG TANAH Sholeh Avivi, Ida Sugeng Suyani & Sugeng Winarso .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 10 No. 1 (2010): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.116 KB) | DOI: 10.23960/j.hptt.11064-72

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Effect of phosphate solubilizing microorganism to Aspergillus flavus development on peanut germination. Phosphate solubilizing microorganisms (PSM) are microorganisms which could increase the available P in plant media. The aim of this research is to determine the effect of PSM on A. flavus growth, and to identify it’s effects on the growth dynamics of A. flavus as long as the peanut germination. The research were conducted in the Laboratory of Plant Diseases Department, Faculty of Agriculture, University of Jember, from August 2005 to May 2006 and divided in 2 stages: in vitro and germination stage. Base on in-vitro stage we concluded that Bacillus subtilis strain SK had better inhibition toward the growth of A. flavus than Pseudomonas fluorescent strain GM. B. subtilis strain SK could inhibit A. flavus as much as 74%, whereas P. fluorescent strain GM was only reached 60% compared to control. Base on the germination stage, B. subtilis in the desiccator supernatant (BsDS) could increase the dry weight of root up to 24.1 g better than P. fluorescens strain GM and control. We also found that B. subtilis strain SK was more effective to change the availability of P on plant media.
Regeneration of Cocoa Zygotic Embryo Using Kinetin in B5 Media Sholeh Avivi
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.974 KB)

Abstract

The objective of this research was intended to identify the influence of kinetin application on zygotic embryo regeneration of several cocoa plants and examine the most appropriate kinetin concentration for regeneration of several cocoa clones. This research was conducted at Laboratory of Plant Tissue Culture, Agronomy Department, Faculty of Agriculture,Jember University. The research was designed by Completely Randomized Factorial Design within four replications. The first factor was 4 cocoa clones which consisted of DR 1, DR 2, ICS13 and ICS60.The second factor was 5 methods of zygotic embryo regeneration. Result showed that the best response of cocoa clone to kinetin concentration of all examined parameters was shown by DR 1 clone. Morever, on initiation stage, the most appropriate kinetin concentration for regeneration of several cocoa clones was 2 ppm.
Unfolded protein response in rice (Oryza sativa L.) varieties with different level of salt stress tolerance Galang Rizki Ramadhan; Sholeh Avivi; Bambang Sugiharto; Wahyu Indra Duwi Fanata
Indonesian Journal of Biotechnology Vol 26, No 3 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.67039

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Plants activate the unfolded protein response as part of cellular adaptation, thereby maintaining the endoplasmic reticulum homeostasis during external stresses exposure. In this study, we examined the relationship between the degree of salt tolerance and unfolded protein response-related gene expression in India salt-tolerant Pokkali and INPARI 35 varieties compared to the Indica salt-sensitive counterpart IR64 and INPARI 4 varieties.  Our result showed that the salt tolerance of Pokkali and INPARI 35 had been confirmed by their higher survival rate, higher chlorophyll content, lower electrolyte leakage, and lower H2O2 and malondialdehyde content under salt stress conditions. Furthermore, the expression of unfolded protein response genes was highest in INPARI 35, whereas IR64 and INPARI 4 exhibited low gene induction during endoplasmic reticulum stress conditions. Among the four examined varieties the salt tolerant Pokkali surprisingly showed the lowest induction of all examined unfolded protein response-related genes. These results indicated the possibility that unfolded protein response supports the rice plant for adapting to the saline environment.
Peningkatan Produksi dan Kualitas Benih Okra (Abelmoschus esculantus L. Moench) Menggunakan Aplikasi Fosfor dan GA3 Nisa Budi Arifiana; Sigit Soeparjono; Sholeh Avivi
Agriprima : Journal of Applied Agricultural Sciences Vol 4 No 2 (2020): SEPTEMBER
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v4i2.360

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Tanaman Okra menjadi salah satu komoditas yang masih belum besar di Indonesia, namun memiliki jumlah permintaan yang terus bertambah.  Produksi Okra dalam negeri masih fluktuatif sehingga belum memenuhi kebutuhan nasional. Kurangnya produksi polong okra salah satunya disebabkan oleh ketersediaan benih yang masih terbatas. Upaya meningkatkan produksi dan kualitas benih okra melalui aplikasi pupuk fosfor dan GA3. Pupuk fosfor dapat membantu dalam proses metabolisme pertumbuhan tanaman dan sebagai sumber energi untuk pembelahan dan pembesaran sel dalam pembentukan bunga, buah dan biji. Aplikasi GA3 juga mampu meningkatkan sistem metabolisme dan menghasilkan akumulasi fotosistat sehinggamenghasilkan presentase daya tumbuh benih lebih baik.  Penelitian disusun berdasarkan rancangan acak kelompok dua faktor yaitu faktor pertama dosis pupuk Fosfor yaitu, P1 (75kg/ha SP-36), P2 (100kg/ha SP-36), P3 (125kg/ha SP-36) dan P4 (150kg/ha SP-36). Faktor kedua konsentrasi GA3 yaitu, G0 (tanpa aplikasi GA3), G1 (50 mg/l), G2 (100mg/l), dan G3 (150mg/l). Sehingga terdapat 16 kombinasi perlakuan dengan ulangan 3 kali. Data dianalisis menggunakan uji lanjut jarak berganda duncan (α,5%) dan uji beda nyata terkecil (α,5%). Hasil penelitian aplikasi GA3 50 mg/l dapat meningkatkan berat biji per polong 3,77 gram, berat biji per tanaman 49,2 gram dan produksi benih 1,98 ton/ha. Kualitas benih pada presentase benih bernas dapat meningkat hingga 96% pada aplikasi konsentrasi GA3 100 mg/l. Pemberian fosfor 75kg/ha SP-36 dan konsentrasi GA3 50mg/l memberikan interaksi pada daya berkecambah benih 90% dan karbohidrat benih 42%.
Identifikasi Gen Sub1A Pada Varietas Tebu Tahan Genangan Ayu Puspita Arum; Sholeh Avivi
Agriprima : Journal of Applied Agricultural Sciences Vol 4 No 2 (2020): SEPTEMBER
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v4i2.373

Abstract

Tebu dapat tumbuh pada berbagai kondisi lingkungan yang cukup beragam termasuk pada lahan marginal seperti lahan yang sering tergenang. Lahan tergenang dapat menurunkan produksi tebu cukup signifikan. Tujuan penelitian ini adalah menemukan gen toleran kebasahan yang terdapat pada 8 varietas unggul komersial tebu  yang tahan genangan yaitu PS 881, PS 862, PS 882, Kentung, PSJT 941, Bulu Lawang, PS 865 dan Kidang Kencana serta mengidentifikasi kemiripannya dengan gen Sub1A. Dengan diperolehnya gen toleran kebasahan pada varietas tebu  tahan genangan di harapkan varietas tebu tersebut dapat ditanam di lahan yang sering tergenang dan dapat memberikan tambahan sumbangan produksi gula secara Nasional. Sehingga pada akhirnya dapat mengurangi impor yang secara kontinu dilakukan pemerintah setiap tahun dan sebagai solusi peningkatan produksi pangan berbasis lahan daratan dalam menghadapi perubahan iklim. Tahapan untuk memperoleh dan mengidentifikasi gen toleran kebasahan atau tahan genangan adalah pengkondisian lahan tanaman tebu tergenang, isolasi DNA dari tanaman tebu yang tergenang, penentuan konsentrasi dan kemurnian DNA yang diperoleh, konfirmasi DNA yang diisolasi dengan elektroforesis dan identifikasi gen Sub 1 A pada DNA sampel metode PCR dengan primer Sub 1 A 203 R/F dan kontrol Positif DNA tanaman padi transgenik yang mengandung gen Sub 1 A. Hasil kegiatan penelitian dapat disimpukan bahwa 8 varietas unggul komersial tebu  yaitu PS 881, PS 862, PS 882, Kentung, PSJT 941, Bulu Lawang, PS 865 dan Kidang Kencana semuanya mempunyai gen yang mirip dengan gen Sub 1 A. Dengan varietas PS 881 dan Kentung menghasilkan band sekuens DNA target gen Sub 1 A paling tebal.
Pengaruh Dosis Pupuk KCl dan Berbagai Level Penyiraman Terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq.) di Pre-Nursery Moh. Syahrul Munir; Sholeh Avivi; Sigit Soeparjono
Agriprima : Journal of Applied Agricultural Sciences Vol 6 No 1 (2022): MARCH
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v6i1.467

Abstract

Saat ini faktor iklim yang semakin tidak menentu mengakibatkan berkurannya ketersediaan air yang berdampak negatif bagi pertumbuhan tanaman. Penelitian ini dilakukan untuk mengetahui pengaruh dosis pupuk KCl dan berbagai kadar air media tanah dipembibitan kelapa sawit. Penelitian ini dilaksanakan di Desa Sumbersari, Kecamatan Sumbersari, Kabupaten Jember, Jawa Timur mulai April hingga September 2021 menggunakan rancangan acak kelompok faktorial. Percobaan dilakukan untuk meneliti pengaruh dosis KCl dan kadar air media terhadap pertumbuhan bibit kelapa sawit, dosis pupuk KCl terdiri dari 4 level yaitu 0 g, 0,4 g, 0,6 g dan 0,8 g setiap tanaman, kadar air media tanah terdiri dari 3 level yaitu 100%, 70% dan 50% dari kapasitas lapang dengan 3 ulangan. Hasil penelitian diketahui bahwa perlakuan interaksi dua faktor antara kadar air media dan dosis KCl didapatkan hasil tidak berbeda nyata, demikian juga faktor tunggal dosis KCl tidak berbeda nyata, sedangkan faktor tunggal kadar air media menunjukkan pengaruh berbeda nyata. Level perlakuan kadar air 70% didapatkan hasil berbeda nyata pada parameter tinggi tanaman sebesar 18,44 cm, parameter jumlah daun sebesar 3,67 helai, parameter diameter bonggol sebesar 5,63 mm dan leaf area index sebesar 1,72. Hasil analisis lebar pori stomata (μm) pada berbagai kondisi kadar air media menunjukkan nilai rata-rata tertinggi pada kadar air media 70% sebesar 11,88 ± 4,96 dan pada berbagai dosis KCl menunjukkan nilai rata-rata tertinggi pada dosis KCl 0,6 g sebesar 10,83 ± 7,90.  
Co-Authors - Asmuni Abdul Jalil Abdul jalil Adam Ramadhan Adhitya Wardhono Adi Prawoto Akbar, Muhammad Mizan Ilham Alvan Maulana Azzini Anang Syamsunihar Andri Wahyudi Anggira, Desi Aprila Iga Mufidah Arum, Ayu Puspita Arya Wiranegara Aurelya, Lalyta Azmi Saleh Azzarah Bakhroini Habriantono Bambang Kusmanadhi Bambang Sugiharto Budi Kristanto Cacuk Purnomo Cahyani, Ardhya Pramisti Regita Choirunnisa, Eviyanti Chozin, dan Muhammad Colleen M. Higgins Colleen M. Higgins, Colleen M. Dewi Kusuma Wardani Djoko Soejono Dwi Erwin Kusbianto Dyah Ayu Savitri Elsye L. Sisworo Faesol, Nurul Galang Rizki Ramadhan Gatot Subroto HAJRIAL ASWIDINNOOR Haliza, Nurhayadatul Halwiyah, Lailatul Hartita, Margaretha Putri Hoesain, Mohammad Ikrarwati Ikrarwati Imelda, Laela Vita Intansari, Herlindha Angghelinna Devfi Irfa' Yudayantho Kacung Hariyono Ketut Anom Wijaya Kim, Kyung Min KOMARUDIN IDRIS Laksono Trisnantoro Lenny Widjayanthi Maulida, Zulfa Maulidina, Niswah Saffanah Moh. Syahrul Munir Mohammad Nur Khozin Mohammad Ubaidillah Muhammad Ghufron Rosyady Rosyady Muhammad Hazmi Nasir, M Abd Nisa Budi Arifiana Novelia, Eka Putri Parawita Dewanti Pio Tifar Ananda Prabowo, Rachmad Udhi Pradika, Yoga Reno Ayom Prayoga, Mohammad Candra Priyanto Andi Prasetyo Puji Rahayu Puspito, Agung Nugroho Qori’ah, Ciplis Gema Raden Soedradjad Rahmawati Rahmawati Ralf G. Dietzgen Ralf G. Dietzgen, Ralf G. Ratih Apri Utami Reny Fauziah Oetami Restanto, Didik Restanto, Didik Pudji Rifngatul ‘Atiqoh Rusdiana, Riza Yuli Satriyas Ilyas setiyono Setiyono Setiyono Setiyono Setiyono Sigit Soeparjono Sitompul, Novrida Yanti Slameto Slameto Slameto Slameto Sobah, Naqiyyah Nada Soetilah Hardjo Soedarmo Sri Hartatik Sri Hartatik Subroto, Gatot Sudarsono Sudarsono . Sudarsono Sudarsono Sugeng Santoso Sugeng Winarso Suliswanto, Eko Nur Suud, Hasbi Mubarak Tri Agus Siswoyo TRI HANDOYO Tri Widyastuti Ubaidillah, Mohammad Wahju Q. Mugnisjah Wahyu Indra Duwi Fanata Wibisono, Viki Bayu Wulanjari, Distiana Yulianti, Annisa Zahrosa, Dimas Bastara Zakariyya, Fakhrusy