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Correlation Between Secondary Metabolites of Leaf and the Resistance to Leaf Rust (Hemileia vastatrix) on Several Arabica Coffee Clones Gatot Subroto; Dwi Erwin Kusbianto; Sholeh Avivi; Slameto Slameto; Setiyono Setiyono
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.61 KB) | DOI: 10.22146/ipas.42124

Abstract

Indicator of coffee resistance to leaf rust attack (Hemileia vastatrix) is needed to select superior coffee plants resistant to biotic stress. This study aims to find the relationship between the content of secondary metabolites and the intensity of leaf rust attack, so that it becomes a reference in the selection of future coffee plants. The experimental design used was a completely randomized design (CRD) with several coffee clones as a treatment. This test tested 5 Arabica coffee clones consisting of Komasti, Maragogik, Usda, Andong sari, and HDT clones. Each consists of 3 replications, and each replication consists of 2 sample plants. Observations were made by observing the intensity of the attack, and plant metabolites such as phenolic content, flavonoids and antioxidant activity were observed when the leaves had been attacked by Hemileia vastatrix. Correlation of leaf rust attack levels with phenolic content, flavonoids and antioxidant activity showed a relationship between each observation variable. Flavonoid content in certain conditions can be used as an indicator to get Arabica coffee plants that are resistant to the attack of leaf rust.
Mikropropogasi Pisang Abaca (Musa textlilis Nee) Melalui Teknik Kultur Jaringan Sholeh Avivi; Ikrarwati Ikrarwati
Jurnal Ilmu Pertanian Vol 11, No 2 (2004): Desember
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.59949

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Pertumbuhan Sawi yang Berasosiasi dengan Bakteri Synechococcus sp. pada Berbagai Kondisi Media Salinitas - Asmuni; Sholeh Avivi; Sugeng Winarso
Agrovigor Vol 10, No 1 (2017): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.915 KB) | DOI: 10.21107/agrovigor.v10i1.2844

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Sawi hijau merupakan salah satu jenis sayur yang di konsumsi oleh masyarakat Indonesia yang memiliki banyak manfaat diantaranya untuk mencegah kanker, hipertensi, penyakit jantung, membantu kesehatan sistem pencernaan, mencegah dan mengobati penyakit pellagra, serta menghindarkan ibu hamil dari anemia. Tanaman sawi pada umumnya dapat hidup di dataran rendah maupun dataran tinggi, akan tetapi lebih baik penanamannya dilakukan di dataran tinggi. Penelitian tentang resistensi sawi di media salin belum banyak dilakukan. Tujuan dari penelitian ini adalah untuk mengetahui respon tanaman sawi hijau yang telah berasosiasi dengan bakteri Synechococcus spterhadap berbagai kondisi salinitas. Penelitian ini dilakukan mulai bulan November 2016 sampai Januari 2017 di Desa Bendoarum Kecamatan Wonosari Kabupaten Bondowoso. Adapun bahan tanam yang digunakan adalah sawi, biakan bakteri fotosintetik (Synechococus sp), air, tanah, pasir, garam NaCl, pupuk (urea, SP-36 dan KCl). Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 4 kali ulangan. Perlakuan salinitas diberikan berdasarkan penambahan konsentrasi garam (NaCl) yaitu kontrol (0 ppm), 2500 ppm, 5000 ppm, 7500 ppm, dan 10.000 ppm. Respon ta tanaman terhadap perlakuan didasarkan pada variabel jumlah daun, jumlah klorofil, panjang akar, volume akar, berat basah, dan berat kering. Data tersebut dianalisis menggunakan uji Anova dan Jarak Berganda Duncan (DMRT) dengan signifikansi pada taraf 5%. Respon tanaman sawi terhadap penambahan garam menunjukkan hasil yang berbeda nyata pada semua parameter pertumbuhan yang meliputi jumlah daun, jumlah klorofil, panjang akar, volume akar, berat basah, dan berat kering. Respon tanaman yang tercekam garam baik yang diberikan bakteri Synechococcus sp atau yang tidak diberikan menunjukkan hasil menurun dan berbeda nyata pada parameter panjang akar, berat basah, dan berat kering. Pemberian bakteri Synechococcus sp pada tanaman yang ditanam dilahan salin tidak berpengaruh nyata pada parameter pertumbuhan tanaman, tapi cenderung meningkatkan berat basah tanaman, berat kering tanaman, dan panjang akar tanaman.Kata kunci : Salinitas, Sawi Hijau, Synechococcus sp, pertumbuhan tanaman
Pengaruh cekaman genangan terhadap pertumbuhan dan hasil beberapa varietas tomat (Lycopersicum esculentum Mill.) Sholeh Avivi; Aprila Iga Mufidah; Tri Agus Siswoyo; Didik Pudji Restanto
Agrovigor Vol 15, No 1 (2022): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v15i1.8866

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Usaha untuk meningkatkan produksi tomat pada lahan tercekam genangan dapat dilakukan dengan menguji varietas-varietas tomat yang sudah beredar terhadap cekaman genangan. Uji dilakukan dengan mengamati parameter pertumbuhan dan hasil beberapa varietas tomat. Penelitian ini bertujuan untuk mengetahui toleransi beberapa varietas tomat terhadap cekaman genangan. Metode yang digunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK) faktorial dengan 3 kali ulangan. Varietas tanaman tomat merupakan faktor pertama dengan 5 taraf yaitu a) V1 Sakina F1, b) V2 Tora, c) V3 Rewako F1, dan d) V4 Mawar. Sedangkan faktor yang kedua adalah cekaman genangan dengan 3 taraf, yaitu a) T1, perlakuan kondisi cekaman genangan 5 cm, b) T2, perlakuan kondisi cekaman genangan 10 cm, c) T3, perlakuan kondisi cekaman genangan 15 cm. Hasil penelitian menunjukkan bahwa varietas Rewako mempunyai karakter lebih toleran terhadap genangan yang ditunjukkan pada parameter rerata tinggi tanaman, ukuran diameter buah, berat buah dan berat tajuk atas tanaman. 
Efektivitas Gen CP PSTV dalam Memproteksi Nicotiana benthamiana Transgenik TO terhadap Serangan Peanut Stripe Virus Sholeh Avivi; Sudarsono Sudarsono; Satriyas Ilyas; Hajrial Aswidinnoor
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12103

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The aims of this research were: (1) to obtain the transgenic Nicotiana benthamiana T0 which have various cp Peanut stripe virus (PStV) genes construct (pBINRCP 1, pBINRCP2, pBINRCP3, and pBINRCP4); (2) to investigate the resistance of transgenic N. benthamianaT0 toward PStV infection; (3) to investigate the effectiveness of those construct to protect N. benthamiana T0 toward PStV infection. To achieve those objectives N. benthamiana T0 (70 plants) were regenerated, PCR tested and infected with PStV using biological analysis methods. The result showed that all of PStV construct gave the resistancy toward PStV infection. The phenotypic respon of those plants were the PBINRCPl plants 35.7% resistance, 0% recovery, and 64.3% susceptible, the PBINRCP2 plants 41.7%, resistance, 0% recovery, and 58,3% susceptible, the PBINRCP3 plants 71.4% resistance, 0% recovery, and 28.6% susceptible, and the PBINRCP4 plants 25.0% resistance, 25.0% recovery, and 50.0% susceptible.
MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERS OF CASSAVA (MANIHOT ESCULENTA CRANTZ) WHICH WET TOLERANT Rahmawati Rahmawati; Tri Agus Siswoyo; Didik Puji Restanto; Sri Hartatik; Sigit Soeparjono; Sholeh Avivi
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Weather conditions often unstable changed and increase the floods of agricultural land. Land which was originally drought will experience wetness and need variety of a plant that wetness tolerant. This study were aimed to get cassava that wet tolerant and to study the morphological, physiological characters of cassava grown on wetness land. The experiment was conducted based on factorial random plot design that consisting two factors with five replications. The first factor were four varieties namely: V1 = Daun Ganja; V2 = Sawi Ketan; V3 = Kasesat Beracun; V4 = Gajah. The second factor was wet treatment consisting of 2 the condition that were K0 = as control, media with 100% field capacity; K1 = as wet treatment by watering of media maintain on -10 cm from media surface. The difference between treatments tested with Duncan Multiple Range Test (DMRT) with the probability 5%. Wet treatment started when plant aged was 2 months after planting, during eight weeks. The observations on variables based on morphological and physiological characters. Result showed that every varieties give the different response on wet treatment. Best response on wet tolerant variety shown by variety code of V2 (Sawi Ketan) were indicated by plant height, total leaf number produced, stem diameter, fresh weight of root, dry weight of root, fresh weight of shoot, dry weight of shoot, photosynthesis index, stomata conductivity and root volume.
The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment Sholeh Avivi; Ralf G. Dietzgen; Colleen M. Higgins; Sudarsono .
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

The aims of this research were to (1) compare the effectiveness of established Agro bacterium mediated transformation and bombardment mediated transformation on peanut (2) evaluate the integration of green fluorescent protein (GFP) and hygromycin resistance (hygr) gene on the peanut embryosomatics. To achieve those objectives we shoot 1200 explants using bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both of Agrobacterium mediated transformation and particle bombardment could give positive embryos transformed.  Particle bombardment gave 4.5% transformation efficiency while Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that particle bombardment more efficient and gave more transgenic explants than Agrobacterium mediated transformation. Keywords: agrobacterium, bombardment, GFP.
PENGARUH PERLAKUAN SORTASI, NATRIUM HIPOKLORIT DAN FUNGISIDA PADA KACANG TANAH UNTUK MENGELIMINASI KONTAMINASI ASPERGILLUS FLAVUS Sholeh Avivi .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 5 No. 1 (2005): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.656 KB) | DOI: 10.23960/j.hptt.1558-65

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The Effect of Sorting, Sodium hypochloride (NaClO), and Fungicide Treatment on Peanut in Elimination the Aspergillus flavus Contamination. The humid tropical condition of Indonesia, the post harvest treatment, and the storage practices favour rapid growth and wide spread Aspergillus infestation on peanut. The objectives of this research was to find the best technique in controlling the Aspergillus infection on peanut. To achieve those objectives, complete randomised design in factorial with three factors was applied. The factors were grading and colour sorting (A1 = grading and colour sorting; A2 = without grading and colour sorting), NaClO blanching (B1 = without blanching; B2 = 1.25% NaClO blanching; B3=2.5% NaClO blanching), and Benlate T-20 WP treatment (C1 = 2,5 g/L; C2 = 3 g/L; C3 = 3,5 g/L). The result showed that the best combination treatment was A2B2C3 with the minimum amount of spore on peanut seed. With those combination treatment we concluded that the grading and colour sorting, the 1.25% NaClO blanching, and the application of 3,5 g/L Benlate T-20 WP could reduced the Aspergillus contamination up to 80% compare with A1B1C3 treatment.
EFEK BAKTERI PELARUT FOSFAT TERHADAP PERTUMBUHAN ASPERGILLUS FLAVUS PADA PERKECAMBAHAN KACANG TANAH Sholeh Avivi, Ida Sugeng Suyani & Sugeng Winarso .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 10 No. 1 (2010): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.116 KB) | DOI: 10.23960/j.hptt.11064-72

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Effect of phosphate solubilizing microorganism to Aspergillus flavus development on peanut germination. Phosphate solubilizing microorganisms (PSM) are microorganisms which could increase the available P in plant media. The aim of this research is to determine the effect of PSM on A. flavus growth, and to identify it’s effects on the growth dynamics of A. flavus as long as the peanut germination. The research were conducted in the Laboratory of Plant Diseases Department, Faculty of Agriculture, University of Jember, from August 2005 to May 2006 and divided in 2 stages: in vitro and germination stage. Base on in-vitro stage we concluded that Bacillus subtilis strain SK had better inhibition toward the growth of A. flavus than Pseudomonas fluorescent strain GM. B. subtilis strain SK could inhibit A. flavus as much as 74%, whereas P. fluorescent strain GM was only reached 60% compared to control. Base on the germination stage, B. subtilis in the desiccator supernatant (BsDS) could increase the dry weight of root up to 24.1 g better than P. fluorescens strain GM and control. We also found that B. subtilis strain SK was more effective to change the availability of P on plant media.
Regeneration of Cocoa Zygotic Embryo Using Kinetin in B5 Media Sholeh Avivi
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.974 KB)

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The objective of this research was intended to identify the influence of kinetin application on zygotic embryo regeneration of several cocoa plants and examine the most appropriate kinetin concentration for regeneration of several cocoa clones. This research was conducted at Laboratory of Plant Tissue Culture, Agronomy Department, Faculty of Agriculture,Jember University. The research was designed by Completely Randomized Factorial Design within four replications. The first factor was 4 cocoa clones which consisted of DR 1, DR 2, ICS13 and ICS60.The second factor was 5 methods of zygotic embryo regeneration. Result showed that the best response of cocoa clone to kinetin concentration of all examined parameters was shown by DR 1 clone. Morever, on initiation stage, the most appropriate kinetin concentration for regeneration of several cocoa clones was 2 ppm.
Co-Authors - Asmuni Abdul jalil Abdul Jalil Adam Ramadhan Adhitya Wardhono Adi Prawoto Akbar, Muhammad Mizan Ilham Alvan Maulana Azzini Anang Syamsunihar Andri Wahyudi Anggira, Desi Aprila Iga Mufidah Arum, Ayu Puspita Arya Wiranegara Aurelya, Lalyta Azmi Saleh Azzarah Bakhroini Habriantono Bambang Kusmanadhi Bambang Sugiharto Budi Kristanto Cacuk Purnomo Cahyani, Ardhya Pramisti Regita Choirunnisa, Eviyanti Colleen M. Higgins Colleen M. Higgins, Colleen M. dan Muhammad Chozin Dewi Kusuma Wardani Djoko Soejono Dwi Erwin Kusbianto Dyah Ayu Savitri Elsye L. Sisworo Faesol, Nurul Galang Rizki Ramadhan Gatot Subroto HAJRIAL ASWIDINNOOR Haliza, Nurhayadatul Halwiyah, Lailatul Hartita, Margaretha Putri Hoesain, Mohammad Ikrarwati Ikrarwati Imelda, Laela Vita Intansari, Herlindha Angghelinna Devfi Irfa' Yudayantho Kacung Hariyono Ketut Anom Wijaya Kim, Kyung Min KOMARUDIN IDRIS Laksono Trisnantoro Lenny Widjayanthi Maulidina, Niswah Saffanah Moh. Syahrul Munir Mohammad Nur Khozin Mohammad Ubaidillah Muhammad Ghufron Rosyady Rosyady Muhammad Hazmi Nasir, M Abd Nisa Budi Arifiana Novelia, Eka Putri Parawita Dewanti Pio Tifar Ananda Prabowo, Rachmad Udhi Pradika, Yoga Reno Ayom Prayoga, Mohammad Candra Priyanto Andi Prasetyo Puji Rahayu Puspito, Agung Nugroho Qori’ah, Ciplis Gema Raden Soedradjad Rahmawati Rahmawati Ralf G. Dietzgen Ralf G. Dietzgen, Ralf G. Ratih Apri Utami Reny Fauziah Oetami Restanto, Didik Restanto, Didik Pudji Rusdiana, Riza Yuli Satriyas Ilyas Setiyono Setiyono Setiyono Setiyono Sigit Soeparjono Sitompul, Novrida Yanti Slameto Slameto Slameto Slameto Sobah, Naqiyyah Nada Soetilah Hardjo Soedarmo Sri Hartatik Subroto, Gatot Sudarsono Sudarsono . Sudarsono Sudarsono Sugeng Winarso Sugeng, Santoso Suliswanto, Eko Nur Suud, Hasbi Mubarak Tri Agus Siswoyo TRI HANDOYO Tri Widyastuti Ubaidillah, Mohammad Viki Bayu Wibisono Wahju Q. Mugnisjah Wahyu Indra Duwi Fanata Wulanjari, Distiana Yulianti, Annisa Zahrosa, Dimas Bastara Zakariyya, Fakhrusy Zulfa Maulida