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Effect of Light Intensities and Nitrogen Fertilizer Dosages on Growth, Phenolics, and Flavonoid Production of Adenostemma lavenia Anisya Elsa Shafira; Sandra Arifin Aziz; Muhammad Farid; Taopik Ridwan; Irmanida Batubara
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 13, No 1 (2024): March 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i1.114-123

Abstract

Adenostemma lavenia (Asteraceae) is a medicinal plant considered a weed, consisted a lot of secondary metabolites, including phenolic and flavonoid. This species has been widely distributed in various countries but has yet to be widely cultivated. Thus, this study aimed to determine the highest plant growth, phenolic, and flavonoid production from A. lavenia cultivated under different shade of nitrogen fertilizers. The experiment used a nested design consisting of 2 factors. The first factor was shade with different intensities (0, 25, 50, and 75%) and the second was nitrogen fertilizer with different doses (0, 45, and 90 kg/ha). The highest plant was achieved at 50% shade level, while the highest leaf number and branch were 25% and 0%, respectively. Leaf thickness and stomata number increased in no-shade conditions. The production of phenolics and flavonoids was high under shaded conditions, supported by significantly high phenolics and flavonoid total under shade conditions, i.e., 35.94 mol gallic acid equivalent/plant and 21.76 mol quercetin equivalent/plant, respectively. A 90 kg/ha nitrogen fertilizer dose produced the best plant growth, phenolic, and flavonoid production. Keywords: Asteraceae, Heatmap, Nested design, Shade.
Correlations between Leaf Nitrogen, Phosphorus and Potassium and Leaf Chlorophyll, Anthocyanins and Carotenoids Content at Vegetative and Generative Stage of Bitter Leaf (Vernonia amygealina Del.) Betty Tjhia; Sandra Arifin Aziz; Ketty Suketi
Journal of Tropical Crop Science Vol. 5 No. 1 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (648.936 KB) | DOI: 10.29244/jtcs.5.1.25-33

Abstract

Vernonia amygdalina Del. (Asteraceae) is a nutritional and medicinal plant that is used widely throughout tropical Africa and other countries. The leaves are proved to have strong antioxidant property. The concentration of chlorophyll a, chlorophyll b, anthocyanins and carotenoids in five different leaf positions of vegetative and generative phases were studied. The aim of the study is to understand and to correlate the content of the three nutrients and the bioactive compounds along the five leaf positions at vegetative and generative stage of the crop growth. The study showed that the concentration of chlorophyll a and b was higher, and anthocyanin concentration was almost zero in the generative than in the vegetative stage. The leaf concentration of N, P, and K were higher in vegetative than in generative stage. At vegetative stage, N positively correlated with chlorophyll a at the 1st leaf; K had strong correlations with anthocyanins at the 2nd and 3rd leaf and with chlorophyll a at the 2nd and 5th, and with chlorophyll b and carotenoids at the 5th leaf. At the generative stage, P had a strong positive correlation with carotenoids at 4th and 5th leaves, and K positively correlated with chlorophyll a and b at 5th leaf. The findings suggest that bitter leaf is better to be harvested at vegetative stage, and that potassium level is important to increase ­anthocyanins and carotenoids content.
NATURAL DYE OF INDIGOFERA TINCTORIA FOR TEXTILE INDUSTRY Arista, Nor Isnaeni Dwi; Aziz, Sandra Arifin; Kurniawati, Ani
Journal of Environmental Science and Sustainable Development Vol. 7, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The examination of sustainability within the textile industry necessitates an exploration of various environmental pollution issues. This study aims to tackle challenges in the synthetic dye industry by reviewing past research on textile dyeing, providing scientific insights, identifying patterns, and highlighting research gaps. It is hypothesized that the cultivation of Indigofera tinctoria with optimized NPK fertilization can significantly enhance its growth and yield, contributing to sustainable natural dye production. Using a mixed-methods approach, this study integrated bibliometric analysis of 1320 Scopus articles with field experiments. The bibliometric analysis, conducted with Vos Viewer software, identifies India and the United States as leaders in textile dye research. Key terms include indigo, indigo carmine, I. tinctoria, and natural dyes. A significant research gap is identified in the cultivation of I. tinctoria, particularly concerning the application of NPK (16:16:16) fertilization. Field experiments analyzed using R-studio demonstrate that NPK significantly increases the fresh weight of I. tinctoria at 11 weeks after planting. Pearson correlation analysis showed a significant correlation between the fresh weight of I. tinctoria on the dry weight of the plants (0.91***) and specific leaf weight (0.60*). These results indicate that advancing research on natural dye indigotin and the cultivation of I. tinctoria plants in the field is highly relevant to addressing research questions concerning the development of natural dye research and the cultivation methods of indigo dye plants in field conditions. NPK fertilization enhances the growth and yield of I. tinctoria, supporting sustainable cultivation in Indonesia. This research contributes to present a viable strategy for promoting sustainable natural dye production and addressing the identified research gap in the cultivation of this important dye-producing species. Future research should focus on optimizing NPK fertilization strategies to maximize yield and sustainability.
PHENOLIC AND FLAVONOID PRODUCTION, PHYTOCHEMICAL PROFILE, AND ANTIOXIDANT CAPACITY OF Adenostemma platyphyllum AT DIFFERENT CONCENTRATIONS OF HYDROPONIC SOLUTIONS : Produksi Fenolik dan Flavonoid, Profil Fitokimia, dan Kapasitas Antioksidan Adenostemma platyphyllum pada Konsentrasi Larutan Hara Hidroponik Berbeda Tamsin, Aqlia Hanna Nurfatiha; Batubara, Irmanida; Ridwan, Taopik; Trivadila; Aziz, Sandra Arifin
Jurnal Tumbuhan Obat Indonesia Vol. 16 No. 1 (2023): July 2023
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v16i1.552

Abstract

Adenostemma platyphyllum is considered a weed with various benefits but has not been widely cultivated. This work aims to obtain the optimum concentration of a nutrient solution (AB-mix) to produce optimum phenolic and flavonoid levels, antioxidant capacity and metabolite profiling in A. platyphyllum using a hydroponic wick system. Different AB-mix nutrient solution concentrations were employed in a one-factor randomized block design. The highest total phenolic, flavonoid concentrations and antioxidant capacity were found in plants without additional nutrient solutions. However, the highest phenolic and flavonoid productivity was found in plants with a nutrient solution concentration of 1100 mg/L. The phenolic/flavonoid group compounds were successfully identified by separating the components using thin-layer chromatography. The intensity of the bands produced from each treatment was quite varied. The untreated plant produced thicker phenolic/flavonoid bands than the other treatments. This was supported by the heatmap pattern produced by the untreated ones, which had high color intensity. Therefore, the optimum concentration of nutrient solution to produce optimum phenolic, flavonoid levels, and antioxidant capacity in A. platyphyllum cultivation by hydroponic wick system was the concentration of 1100 mg/L.
Utilizing Rice Hull Ash and Biomass as Ameliorants Enhanced Soybean Yield and Nutrient Uptake in Tidal Swamps Under Saturated Soil Culture Toyip, Toyip; Ghulamahdi, Munif; Sopandie, Didy; Aziz, Sandra Arifin; Sutandi, Atang; Jarwadi Purwanto, Mohamad Yanuar
PLANTA TROPIKA Vol 12, No 1 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i1.18486

Abstract

Saturated soil culture (SSC) is a promising technology for cultivating soybeans in acidic soil in tidal swamps. The objectives of this study were to measure nutrient uptake in the soybean plant using a saturated soil culture technique and to assess the impact of ameliorant addition in the form of rice biomass and rice hull on soybean yield. Two factors, rice biomass, and rice hull ash, were used in a split plot factorial design to set up the experiment. Four levels in the main factor included without biomass immersion, one immersion of short (3 cm above the root) rice stubble, one immersion of regular (panicle harvested only) rice straw, and two immersions of regular rice straw cut in the soil. The four levels in the subfactor were 0 t ha-1, 0.25 t ha-1, 0.50 t ha-1, and 0.75 t ha-1 of rice hull ash doses. Ameliorant in the form of biomass and rice husk increases soil quality in tidal swamps by enhancing pH, soil nutrients (N, P, K, S), CEC, cations, and base saturation, also reducing toxic compounds and improving soil texture. Application of ameliorant also increases soybean nutrient uptake and eventually increases the productivity of soybeans in tidal swamp acid soil. The best treatment of the first and second factors was one immersion of biomass with a regular cut of rice straw and 0.5 t ha-1 ash rice hull ash addition to the tidal swamp soil, respectively. 
Modification of The Spectrophotometric Analysis Protocol by Sims D. and Gamon to Analyze Leaf Pigment Content using Green Spinach (Amaranthus hybridus) as a Model Plant Yudiansyah, Yudiansyah; Ismiyanti, Ismiyanti; Aziz, Sandra Arifin
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.147-154

Abstract

The analysis of pigment content in plants, particularly chlorophyll a, chlorophyll b, anthocyanin, and carotenoids, is crucial to assess their physiological performance. The spectrophotometry method by Sims and Gamon offers practicality and reliable results. This research established several variables to achieve more precise results. Green spinach leaf samples were used as a model to analyze their pigment content, determining the minimum sample area or weight, the maximum limit that does not violate Beer’s law, and the temperature during analysis. The limit of quantitation (LoQ) is the smallest quantity of analytes in a sample that still meets the criteria for accuracy and precision in testing. The research results show that using a leaf punch with a diameter of 9 mm approaches the LoQ value. To avoid measurements nearing the LoQ limit, it is advisable to use leaf punch diameters of 10- or 12-mm. Leaf punches of 22 mm are still safe to use, as there has been no deviationfrom Beer’s law. Using unchilled acetone at 27°C did not show significant differences compared to using cold acetone at 7°C for the content of chlorophyll a, total chlorophyll, and carotenoids. However, for chlorophyll b and anthocyanin, despite significant differences, the chlorophyll content measured at room temperature was more practical and relatively higher compared to cold acetone, thus disproving concerns about pigment damage.
Dynamics of Nutrient Concentrations, Endogenous Hormones, Photosynthetic Capacity, and Phenological Changes in Black Orchid (Coelogyne pandurata Lindl.) from the Vegetative to Generative Phase Heriansyah, Pebra; Aziz, Sandra Arifin; Sukma, Dewi; Nurcholis, Waras
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.215-234

Abstract

The black orchid (Coelogyne pandurata Lindl.) is one of the native Indonesian orchids from Borneo island. This study analyzed the dynamics of pigments, photosynthetic capacity, endogenous hormones, nutrient concentrations, and phenology across the vegetative to generative stages of the black orchid. The plant materials are one-year-old plants after splitting from the mother plants. Endogenous hormones, nutrient and pigment concentrations, and leaf photosynthetic capacity were measured during vegetative and generative phases. Chlorophyll, anthocyanins, and carotenoids were analyzed using UV-VIS spectrophotometry, Nitrogen (N) was analyzed by the Kjeldahl method, phosphorus (P) and potassium (K) by the Bray 1 method and Flame Photometry, and organic carbon by the Walkley- Black method with titration. The hormone levels were analyzed using HPLC, and photosynthetic capacity was determined using a Li-Cor 6800 system. Phenological changes in leaves, bulbs, and flowers were recorded. Results showed total chlorophyll increased from 1.96 to 2.36 mg/g from the vegetative to the generative stage while carotene slightly declined. Leaf nitrogen rose from 1.54% to 1.70%, bulb nitrogen decreased from 0.67% to 0.45%, whereas the C/N ratio increased from 65.24 to 85.36 from the vegetative to the generative phase. Flower nitrogen was 1.50%, and phosphorus was 0.17%. IAA in leaves decreased from 25.12 to 21.37 ng.g⁻¹ dry weight post-flowering, while gibberellin in bulbs increased from 12.28 to 12.96 ng.g⁻¹ dry weight. Zeatin in bulbs declined from 10.52 to 8.81 ng g⁻¹ dry weight. Photosynthesis peaked at 2.73 μmol. m⁻².s⁻¹ in early generative stages and then declined. Photosynthetic photon flux density and stomatal conductance decreased, reducing water and CO₂ exchange efficiency, while net assimilation and transpiration rates showed no significant changes. These physiological adjustments, including increased chlorophyll levels, changes in nitrogen allocation, and fluctuations in hormone concentrations, reflect the plant’s adaptive strategies to meet higher energy demands during reproductive growth, ensuring efficient resource distribution for flowering.
Optimizing Growth and Flavonoid Production in Kaempferia angustifolia Using Organic (Chicken and Cow Manure) and Inorganic Fertilizers Aziz, Sandra Arifin; Ridwan, Taopik; Iswantini, Dyah; Trivadila, Trivadila; Murni, Anggia; Rafi, Mohamad
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.365-377

Abstract

Kaempferia angustifolia originated in Southeast Asia and is widely used for its medicinal properties. One of which is from its flavonoids. The research aims to determine the optimal dosage of organic and inorganic fertilizers for promoting the growth and flavonoid production of K. angustifolia. The study was laid out in a split-plot design using organic fertilizers as the main plots (chicken and cow manure at 10 t.ha-1) and inorganic fertilizers as the subplots, consisting of 19 combinations of urea, SP36, and KCl. Each treatment has three replications.  The results showed that there is no interaction between organic and inorganic fertilizers in affecting the growth of Kaempferia angustifolia. Plants treated with chicken manure at 10 tons per hectare have a higher fresh weight than those treated with cow manure. Plants without inorganic fertilizer application showed suppressed growth and yielded more rhizomes three months after planting. Inorganic fertilizer promoted more shoots and tillers, with the highest value obtained from 100 kg.ha-1 urea + 200 kg.ha-1 SP36 + 200 kg.ha-1 KCl application.   The range of total flavonoids in inorganic fertilizer applications is 39.30-131.51% higher than the control, with the highest value observed at 200 kg.ha-1 SP36 + 200 kg.ha-1 KCl application. The results of this study would be useful for producing K. angustifolia for the medicinal industry, using either organic or inorganic fertilizers.
INSTANT GRANULES OF Zingiber officinale AND Cajanus cajan LEAVES IMPROVED LIVER HISTOLOGICAL PROFILE IN DIABETIC MODEL RATS Manalu, Johanes Marojahan; Sadiah, Siti; Aziz, Sandra Arifin; Astawan, Made; Ridwan, Taopik; Wresdiyati, Tutik
Jurnal Kedokteran Hewan Vol 19, No 3 (2025): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i3.47330

Abstract

Diabetes mellitus can cause significant microstructural damage in liver tissue. This study evaluated the effectiveness of instant granules of Zingiber officinale and Cajanus cajan leaves on the liver histological profile in diabetic model rats. A total of 25 male Sprague Dawley rats were assigned into five groups. The group consist of negative control (NC), positive control (PC), metformin control group at a dose of 150 mg/kg BW (MET), and two treatment groups of instant granules of Z. officinale and C. cajan leaves at a dose of 300 mg/kg BW (T1) and 150 mg/kg BW (T2) for 28 days treatment. The diabetic condition was obtained by single induction of streptozotocin (35 mg/kg BW). The liver tissue was stained using hematoxylin-eosin staining to observe the cytoplasm intensity, central vein diameter, and nucleus intensity of liver cells in diabetic model rats. The results showed that administration of the instant granules significantly decrease liver microstructural damage (P0.01). The 150 mg/kg BW (T2) dose showed the most optimal effectiveness by successfully decreasing cytoplasmic intensity, reducing central vein dilation, and decreasing nuclear cell intensity. The research result indicate that instant granules have strong hepatoprotective properties that can repair liver microstructural damage caused by diabetes.
The Effect of Heavy Pruning and Organic Fertilizer to Biomass and Flavonoid Production Orange Jessmine Leaves at the First and Second Harvest Eman Ayu Sasmita Jati, Gusti; Arifin Aziz, Sandra; Melati, Maya
Jurnal Hortikultura Indonesia (JHI) Vol. 10 No. 2 (2019): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.409 KB) | DOI: 10.29244/jhi.10.2.135-144

Abstract

Pemanenan kemuning (Murraya paniculata (L.) Jack.), dengan cara pangkas di ketinggian yang sama terus-menerus dapat menyebabkan penurunan produksi. Penerapan pangkas berat terhadap tanaman kemuning belum pernah dilaporkan sebelumnya. Penelitian ini bertujuan untuk menentukan ketinggian pangkas dan dosis pupuk yang sesuai untuk mempertahankan atau meningkatkan produksi daun kemuning dan flavonoid. Penelitian dilakukan dari bulan Juli 2015 sampai Juli 2016. Percobaan dilakukan di Kebun Percobaan Organik Institut Pertanian Bogor di Cikarawang, Bogor. Percobaan menggunakan rancangan acak kelompok dengan dua faktor dan empat ulangan. Faktor pertama adalah tinggi pemangkasan yang terdiri atas 45, 60 dan 75 cm di atas permukaan tanah. Ketinggian 75 cm didesain sebagai kontrol. Faktor kedua adalah kombinasi dosis pupuk kandang ayam (PA) dan abu sekam (AS), yaitu: tanpa pupuk (kontrol), 7 kg PA + 3 kg AS, dan 14 kg PA + 6 kg AS per tanaman per tahun. Panen dilakukan dua kali dengan interval empat bulan. Hasil penelitian menunjukkan bahwa ketinggian pangkas 60 cm di atas permukaan tanah merupakan pemangkasan berat terbaik untuk pertumbuhan, produksi, dan produksi total flavonoid daun tanaman kemuning yang berusia 45-57 bulan setelah tanam (BST) setelah 4-6 kali panen, sementara perlakuan pemupukan tidak memberikan hasil yang berbeda nyata. Kata kunci: abu sekam, kandungan pigmen, pupuk kandang ayam, tinggi pangkasan
Co-Authors , Adiwirman , Nazi , Nirwan ,, Herlina . Herlina . TRIVADILA A.A. Ketut Agung Cahyawan W Abdul Munif Agus Yudhi Pardiyanto Andika Akbarul Iman Andria Afrida Anggia Murni Ani Kurniawati Anisya Elsa Shafira Ari, Ahmad Nur Hidayat Gena Arista, Nor Isnaeni Dwi Atang Sutandi Auliya Ilmiawati Ayunina, Krisetya Azmi, Tubagus Kiki Kawakibi Bayuanggara Cahya Ramadhan betty betty Betty Tjhia BUDI MARTONO DADANG DADANG Danar Hadisugelar Dardiri, Dardiri Dewi Sukma Dhedy Kristanto Didy Sopandie Diny Dinarti DINY DINARTY, DINY Dyah Iswantini Edi Santosa Ekawati, Rina Eman Ayu Sasmita Jati, Gusti Fajar Pangestu Fauziah, Ni'mah Fhonna, Tasya Nurizki Giyanto, dan Gusmaini Gusmaini GUSMAINI GUSMAINI GUSMAINI GUSMAINI, GUSMAINI Haridjaja, Oteng Herlina, NFN Hesti Pujiwati Hesti Pujiwati Hilda Susanti Hilda Susanti I Ketut Suada Ina Kusheryani Ira Fauziah Noer Irmanida Batubara Ismail Saleh Ismail Saleh Ismiyanti, Ismiyanti Isna Tustiyani Isna Tustiyani Jamil, Anna Moslihat Kailola, Joan Joulanda Grace Karimuna, Siti Rahmah Ketty Suketi Krisetya Ayunina Kurniawati, Ari Kurniawati, Ari LATIFAH K. DARUSMAN Leo Mualim M. Rafi MADE ASTAWAN Manalu, Johanes Marojahan MARIA BINTANG Maya Melati Ma’rufah, Siti Hilda Meity Suradji Sinaga Melati , Maya Miftahudin . Mohamad Rafi Mufa'adi, Ardianto Muhamad Syukur Muhammad Farid Muhammad Farid Muhammad Syukur Munawaroh, Nafi'atul Munif Ghulamahdi Mustika Tripatmasari Tripatmasari Nafi'atul Munawaroh Neni Musyarofah NFN Herlina Ni'mah Fauziah Noer, Ira Fauziah Nur Faridah, dan Didah Nuri Andarwulan Nurliani Bermawie NURLIANI BERMAWIE NURLIANI BERMAWIE Nurliani Bermawie Nurwita Dewi Oteng Haridjaja Parwito Pebra Heriansyah Purwono Purwono, Purwono Purwono, dan Putri, Astrid Aditya Putri, Fiadini Rahmi Taufika Ramadhan, Bayuanggara Cahya Ramadhani, Elrisa Rasmani, Rasmani Ray March Syahadat Refa Firgiyanto Respita, Intan Annisa rina ekawati Rizva, Dian Novira Sadiah, Siti Sari, Galuh Yunita Shafira, Anisya Elsa Siti Hilda Ma’rufah Siti Maryam Harahap Siti Maryam Harahap Siti Sadiah Slamet Susanto Sri Astuti Rais Stefani Daryanto Sudirman Yahya Sudirman Yahya Tamsin, Aqlia Hanna Nurfatiha Taopik Ridwan Taopik Ridwan Tatik Raisawati Toyip Toyip Tutik Wresdiyati Wahyu Arif Sudarsono Wahyu Arif Sudarsono Waras Nurcholis Wardani, Eska Ayu Winarso D. Widodo Yudiansyah Yudiansyah Yulia Indriani