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Agrijanti, Agrijanti
Poltekkes Kemenkes Mataram, Mataram, Nusa Tenggara Barat
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UJI SENSITIVITAS DAN SPESIFITAS UJI PENYARING KANDIDIASIS VAGINA MENGGUNAKAN SEDIMEN DENGAN PENGECATAN GRAM Sundayani, Lina; Agrijanti, Agrijanti
Jurnal Analis Medika Biosains (JAMBS) Vol 2, No 1 (2015): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v2i1.29

Abstract

Pewarnaan Gram adalah metode berharga dalam diagnosis yang akurat cepat gejala vulvovaginal dan bahkan terkadang lebih unggul dari kultur  karena menunjukkan bentuk invasif dari ragi1. Tujuan penelitian untuk mengetahui Sensitivitas dan Spesivitas Uji penyaring Kandidiasis Vagina dengan Menggunakan Sedimen dengan Pengecatan Gram. Penelitian uji sensitivitas dan spesivitas sediaan Gram menggunakan 25 sampel sedimen urin wanita penderita Diabetes Mellitus sebagai Uji Penyaring  kandidiasis vagina Hasil pemeriksaan sedimen urin dibuat sediaan ditambah KOH 20%  negatif sel ragi 16, Positif sel ragi.9Hasil pemeriksaan sedimen urin dibuat sediaan Gram 18 negatif sel ragi dan 7 positif sel ragi.  Hasil kultur sebagai Gold Standar di media Sabaouroud Glukosa Agar dan Corn Meal Agar diperoleh hasil 18 negatif dan 7 Positif sedimen urine yang tumbuh Candida albicans Hasil perhitungan ditemukan Sensitivitas dan Spesifitas pemeriksaan kandidiasis Vagina dengan menggunakan sedimen urin yang ditambah KOH 10% ialah sebagai berikut Sensitivitas 63,63% Spesifisitas 85,71%. Dari hasil Perhitungan maka ditemukan sensitivitas dan spesifitas metode pemeriksaan dengan menggunakan sedimen yang dibuat sediaan gram     Sensitivitas 87,50% Spesifisitas 93,75%. Hasil Sensitivitas dan Spesivitas pemeriksaan dengan menggunakan sedimen yang dibuat sediaan gram lebih tinggi daripada pemeriksaan Kandidiasis Vagina dengan menggunakan sedimen urin yang ditambah KOH 10%Kata Kunci :
Lipid Profile Animal Trial Model of Metabolic Syndrome with Soggak Coffee Intervention Dewi, Lale Budi Kusuma; Permatasari, Lina; Khusuma, Ari; Agrijanti, Agrijanti
Jurnal Mandala Pharmacon Indonesia Vol. 11 No. 1 (2025): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v11i1.712

Abstract

Sindrom metabolik adalah suatu kondisi yang berhubungan dengan obesitas, diabetes, dislipidemia (hiperkolesterolemia dan hipertrigliseridemia), dan tekanan darah tinggi. Stres oksidatif memiliki keterkaitan dengan sindrom metabolik, makanan kaya flavonoid bermanfaat untuk pencegahan dan perbaikan sindrom metabolik. Songgak adalah kopi yang dicampur dengan rempah-rempah dengan kandungan flavonoid dan fenolik total yang berkorelasi dengan aktivitas antioksidannya. Penelitian ini bertujuan untuk menganalisis efek kopi songgak dalam mencegah sindrom metabolik. Parameter yang diuji adalah profil lipid yang terdiri dari kadar kolesterol total, kadar trigliserida, kadar low-density lipoprotein (LDL), dan kadar high-density lipoprotein (HDL). Sebanyak 24 ekor hewan coba dibagi menjadi 4 kelompok (kelompok kontrol, P1, P2, dan P3).  Semua hewan diberikan makanan tinggi, lipid, dan Propylthiouracil (PTU). Kelompok P1, P2, dan P3 masing-masing diberi 8 gram, 12 gram, dan 16 gram kopi Songgak sedangkan kelompok kontrol tidak diberi kopi Songgak. Setelah perlakuan selama 21 hari, hewan coba diterminasi dan profil lipid diukur. Uji statistik dengan Kruskal Wallis pada kadar total kolesterol dan HDL kolesterol dengan hasil uji nilai P berturut-turut adalah 0,205 dan 0,637 (P>0,05), disimpulkan tidak ada perbedaan antar kelompok perlakuan. Sedangkan kadar trigliserida dan LDL diuji dengan uji parametrik ANOVA, hasil uji nilai sig berturut-turut adalah 0,068 dan  0,748 >0,05 disimpulkan tidak ada perbedaan antar kelompok perlakuan. Walaupun menurut uji ststistik disimpulkan tidak ada perbedaan antar kelompok perlakuan, namun kadar kolesterol total, trigliserida, kolesterol LDL dan kolesterol HDL pada kelompok perlakuan lebih baik daripada kelompok kontrol. Kelompok P2 menunjukkan kadar kolesterol total, gliserida, dan LDL yang terendah dengan kadar secara berurutan yaitu 69,67±11,86; 51,00±892; dan 9,33±3,01 mg/dL. Sementara itu, kadar HDL tertinggi pada kelompok P3. Terdapat penurunan kadar kolesterol total, kolesterol LDL, dan trigliserida, serta peningkatan kadar kolesterol HDL pada kelompok perlakuan. Oleh karena itu, kopi songgak dapat dikembangkan menjadi minuman tradisional untuk mencegah sindrom metabolik.
Correlation of Leukocyte Esterase Positivity Levels Against the Results of Several Examination Parameters Laboratory Urinary Tract Infection in Patients at Mataram City Regional Hospital Waisnawa, I Made Wahyu; Wiadnya, Ida Bagus Rai; Manu, Thomas Tandi; Agrijanti, Agrijanti
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.326

Abstract

Background : Urinary tract infection (UTI) is caused by the multiplication of bacteria in the urinary tract. The standard proving procedures for UTI are urine culture examination and urine dipstick examination (leukocyte esterase and nitrite). Objective : This study aims to measure leukocyte and nitrite values, calculate the total number of bacteria, identify Gram type, species and antibiotic sensitivity and analyze the correlation of leukocyte esterase positivity to the results of several UTI examination parameters. Research Methods : The method used is an analytic observational design with cross sectional design using primary data and analyzed with the Spearman rank test. Results : The results showed that in 198 patients with suspected UTI, 123 (62%) were positive for leukocyte esterase, 16 (8.1%) were positive for nitrite. The total number of bacterial countshowed 82 people (41%) with bacteriuria ≥10 5 CFU/mL. Found 61 Gram-negative bacilli and 21 Gram-positive cocci with 21 different bacterial species. Antibiotic sensitivity test showed 51% sensitive, 6% intermediate and 43% resistant. Correlation of leukocyte esterase with nitrites (p 0.023<0.05), total bacteria count (p 0.00<0.05), Gram negative bacilli (p 0.00<0.05), and Gram positive cocci (p 0.533>0.05), antibiotic sensitivity test (p 0.794 >0.05) and bacterial species (p 0.316>0.05). Conclusion : The results showed that there was a correlation between the level of positivity of leukocyte esterase and nitrite, total bacteria count and Gram-negative bacilli, but there was no correlation between Gram-positive cocci, bacterial species and antibiotic sensitivity tests.
Efektivitas Penggunaan Cuka Apel (Apple Cider Vinegar) Terhadap Daya Hambat Pertumbuhan Staphylococcus Aureus Yang Diisolasi Dari Ulkus Diabetes Mellitus Novianty, Alfiah; Agrijanti, Agrijanti; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.200

Abstract

Diabetic ulcers are a chronic complication of Diabetes Mellitus in the form of open sores on the surface of the skin. Staphylococcus aureus bacteria is one of the bacteria that causes infection in wounds. Staphylococcus aureus bacteria will flourish in areas that have infections. An increasingly severe infection will cause gangrene. As it is known that Apple vinegar contains chemicals that are as antibacterial. Therefore apple vinegar can be used as a treatment in inhibiting the growth of Staphylococcus aureus bacteria. This study used a quasi-experimental design with 6 treatments, namely Tetracycline Antibiotics as a negative control of growth, sterile aquadest as a positive control of growth and apple vinegar with concentrations of 100%, 50%, 25%, and 12.5%. Then performed a statistical test using Kruskal Wallis with a confidence level of 95% or α = 0.05. Result: Sample Diabetic Foot Ulcer positively contained Staphylococcus aureus. Test the sensitivity that is done by using vinegar apple effective to inhibit the growth of Staphylococcus aureus at concentrations of 50% with a power resistor of 21.75 mm and a concentration of 100% by 24 mm. Conclusion: Apple Cider Vinegar effective to inhibit the growth of Staphylococcus aureus was isolated from Ulcer Foot Diabetic.
Analysis of Patient Examination Results with Clinical Symptoms of Gonorrhea Using Microscopic Examination at Puskesmas Dasan Agung Widyastini, Ni Komang Ari Santi; Zaetun, Siti; Agrijanti, Agrijanti; Rohmi, Rohmi
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.345

Abstract

Tropical clinical symptoms of gonorrhea occur in men, to confirm a clinical diagnosis then use microscopic examination with gram coloring. The Puskesmas of Dasan Agung became a reference to the inspection of the STI in the city of Mataram. The purpose of this study is to identify clinical symptoms and results of microscopic examination using gram coloring. This type of research is descriptive observational which aims to know or describe the picture of a disease based on direct observation objectively. The population in this study were all patients who came with symptoms that lead to sexually transmitted diseases in January - March, totaling 13 respondents. Sampling was done by non-random purposive sampling method. This research was conducted at the Dasan Agung Health Center laboratory. The results of a study of 13 patients with clinical symptoms of STI obtained 13 positive respondents (100%) identified Neisseria gonorrhoeae. 10 male patients (67.9%) and 3 female patients (23.1%) were identified with heterosexual sexual orientation in 10 cases and 3 were homosexual.
Pengaruh Pencucian Darah Domba dengan NaCl 0,85% Terhadap Pertumbuhan Bakteri Neisseria gonorrhoeae Pada Media CAP Agrijanti, Agrijanti; Intan Suryawatie Yomo, Meidiana; Khusuma, Ari; Kusuma Dewi, Lale Budi
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.259

Abstract

Neisseria gonorrhoeae is a fastidious organism. The growth medium for the bacterial culture of Neisseria gonorrhoeae is a Chocolate Agar Plate (CAP) medium enriched with additional nutrients such as sheep blood, vancomisin, nystatin, and isovitaleX. in general, the making of  CAP  is not washed (defibrinated) the sheep blood, in this research, the sheep blood was washed (defibrinated) with NaCl 0.85%. The purpose of the research is to determine the effect of washed sheep blood with NaCl 0.85% on the growth of Neisseria gonorrhoeae in CAP. The method of this research is a pre-experiment conducted in a laboratory to find out a symptom or effect that arises as a result of the treatment of the sample. The treatment in this research is CAP washed of sheep blood and CAP with unwashed sheep blood as a control. The result is the colonies that grew on CAP treatment were better than colonies that grew on CAP control. The conclusion of this research is there is an effect of washed sheep blood with NaCl 0.85% on the growth of Neisseria gonorrhoeae in CAP medium.
Pemisahan dan Penentuan Karakter Fraksi Protein ESA Toxoplasma gondii Secara ImmunologikUntuk Pengembangan Diagnosa Dewi, Lale Budi kusuma; Agrijanti, Agrijanti
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.216

Abstract

Toxoplasmosis is a zoonotic disease that attacks vertebrates including humans. T gondi infection cause toxoplasmic encephalitis in immunocompromised patients, blindness, abortion, fetal abnormalities and even fetal death or miscarriage. the diagnosis of toxoplasmosis is mainly based on detection of specific antibodies in serum samples using the T gondii antigen protein. various kinds of antigens are explored to be used as bioresptors to detect antibodies against T gondii in the patient's body. the use of ESA protein has advantages in terms of simple preparation. The lacks is  the protein contamination by extraparasitic components so that it can reduce the sensitivity and specificity of T gondii antibodies detection. The purpose of this study is to separate or purify antigenic ESA proteins to increase the sensitivity and specificity detection by purifying ESA proteins with gel filtration chromatography. The results obtained 3 fractionation, the protein ESA fractionation is one of fraction that has antigenic properties towards T Gondii. dipstick test with antigen using fraction 3 the results of ESA protein fractionation, 7 samples were positive from 8 positive samples, gave a sensitivity value of 87.5% and 6 samples were negative from 8 negative samples, giving a specificity value of 75%.
Differences in Erythrocyte Indices in the First and Third Months of Hemodialysis in Chronic Kidney Failure Patients at Praya Regional Hospital Zahira, Jauhara Rana; Agrijanti, Agrijanti; Getas, I Wayan; Zaetun, Siti
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.464

Abstract

Hemodialysis therapy plays a role in replacing the kidneys' performance in filtering toxins and forming the hormone erythropoietin which forms and matures red blood cells. For this reason, it is necessary to conduct an examination of the erythrocyte index which functions to monitor the severity of anemia caused by chronic kidney failure. This study is a descriptive study with a cross-sectional approach. The sampling technique with purposive sampling is a technique with sample determination according to the researcher with a sample of patients with erythrocyte index examination data in the first and third months of undergoing hemodialysis. Data were analyzed using the Wilcoxon difference test. The total number of complete data of erythrocyte index examination in the first and third months was 22 patients. The average value of the first month erythrocyte index MCV 83.58 fL, MCH 28.31 pg, and MCHC 33.06 g/dL. In the third month the average value of the erythrocyte index MCV 85.35 fL, MCH 28.89 pg, and MCHC 33.90 g/dL. The results of the study stated a significance value of p 0.000 for MCV, MCH value p 0.015, and MCHC value p 0.005. There is a difference in the erythrocyte index in the first and third months of chronic kidney failure patients undergoing hemodialysis
Pengaruh Konsumsi Berbagai Jenis Sumber Karbohidrat terhadap Kadar Glukosa Darah 2 Jam PP pada Penderita Diabetes Melitus Tipe 2 Sufina Zikria Putri; Agrijanti; Thomas Tandi Manu; Lalu Srigede
Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 1 No. 2 (2025): Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : PT Pustaka Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71417/galen.v1i2.100

Abstract

Latar Belakang: Diabetes Melitus tipe 2 merupakan salah satu masalah kesehatan yang terus meningkat di Indonesia. Salah satu faktor penting dalam pengelolaan diabetes adalah pengaturan konsumsi karbohidrat karena berpengaruh langsung terhadap kadar glukosa darah Postprandial (2 jam setelah makan). Jenis karbohidrat yang dikonsumsi diduga memiliki pengaruh yang berbeda terhadap respons glukosa darah penderita diabetes. Tujuan Penelitian: Mengetahui pengaruh konsumsi berbagai jenis sumber karbohidrat terhadap kadar glukosa darah 2 jam Postprandial pada penderita Diabetes Melitus tipe 2. Metode Penelitian: Penelitian ini merupakan penelitian kuantitatif dengan desain pre-eksperimen (pre and post test group design). Subjek penelitian terdiri dari 28 orang penderita Diabetes Melitus tipe 2 yang dibagi ke dalam 7 kelompok berdasarkan jenis karbohidrat yang dikonsumsi (beras merah, kentang, singkong, ubi talas, ubi jalar, jagung, dan nasi putih). Data dianalisis menggunakan uji Paired t-Test. Hasil Penelitian: Rata-rata kadar glukosa darah sebelum dan sesudah mengkonsumsi karbohidrat beras merah (434,5 dan 394,5 mg/dL), Kentang (337 dan 185,5 mg/dL), Singkong (447,25 dan 404,75 mg/dL), Ubi Talas (354,25 dan 305,5 mg/dL), Ubi Jalar (311 dan 257,25 mg/dL), Jagung (369 dan 353,75 mg/dL) dan nasi putih (293 dan 315,75 mg/dL). Uji Paired t-Test menunjukkan bahwa kelompok beras merah (p=0,001), kentang (p<0,001), ubi talas (p=0,023), ubi jalar (p=0,008) mengalami penurunan signifikan, sementara singkong dan jagung tidak signifikan (p>0,05). Nasi putih menunjukkan peningkatan signifikan (p=0,008). Kesimpulan: Terdapat pengaruh konsumsi beras merah, kentang, ubi talas dan ubi jalar menunjukkan penurunan signifikan terhadap kadar glukosa darah, sedangkan konsumsi singkong dan jagung mengalami penurunan yang tidak signifikan. Sementara itu, konsumsi nasi putih justru meningkatkan kadar glukosa darah secara signifikan.
Identifikasi Sel Ragi (Candida sp.) pada Sedimen Urine dengan Menggunaka Metode Preparat Basah dan Preparat Gram pada Penderita Diabetes Melitus Cahayaningrum, Aryani; Agrijanti; Manu, Thomas Tandi; Khusuma, Ari
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 1 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i1.75

Abstract

Latar Belakang: Kandidiasis adalah infeksi jamur yang disebabkan oleh jamur Candida sp. Diabetes melitus menjadi salah satu faktor predisposisi terjadinya infeksi jamur. Diagnosa dari kandidiasis bisa dilakukan dengan berbagai macam cara. Salah satunya adalah dengan melihat sel ragi pada sedimen urin di bawah mikroskop dengan menggunakan metode preparat basah atau melakukan pewarnaan gram . Tujuan Penelitian: Mengidentifikasi sel ragi (Candida sp.) pada sedimen urin dengan menggunakan metode preparat basah dan preparat gram pada penderita Diabetes Melitus. Metode Penelitian: Rancangan Penelitian ini merupakan penelitian deskriptif, yang dilakukan dengan metode observasional analitik dengan pendekatan cross sectional sehingga variabel bebas dan variabel terikat diamati dalam satu waktu. Hasil: Didapatkan 8 sampel positif sel ragi dan 14 sampel negatif sel ragi pada metode preparat basah dan preparat gram. Kesimpulan: Berdasarkan kualitas, preparat gram lebih unggul daripada preparat basah karena sel ragi yang terlihat pada preparat gram lebih jelas daripada sel ragi yang terlihat pada preparat gram. Sedangkan dari segi efisensi waktu dan biaya, preparat basah lebih unggul karena cepat dan murah.
Co-Authors Aeni Halawiya Aini, Siti Rahmatul Alfiah Novianty Anisa Prisilia Prisilia Ari khusuma Baiq Lilis Septiana Cahayaningrum, Aryani Dewi, Lale Budi Kusuma Dwi Melbaow Aisyiah Putri Erlin Yustin Tatontos Erna Kristinawati Eustachius Hagni Wardoyo Fadhilah Rizkiyah Fathul Djannah Febriza Marta Utami Fihiruddin Fihiruddin Fihiruddin Getas, I Wayan I Made Wahyu Waisnawa I Wayan Getas I Wayan Getas Imanta Alifia Octavira Intan Suryawatie Yomo, Meidiana Khusuma, Ari Lalu Srigede Lalu Srigede Lalu Srigede, Lalu Lina Sundayani Meidiana Intan Suryawatie Yomo Muhammad Luthfi Ni Komang Ari Santi Widyastini Nispahayati Nispahayati Nova Ramdhani, Iid Nirmala Novianty, Alfiah Nurhidayati Nurhidayati Nurul Inayati Nurul Inayati Nurul Inayati Nurul Inayati Octavira, Imanta Alifia Permatasari, Lina Putriawati Putriawati Putriawati, Putriawati RAI WIADNYA, IDA BAGUS Rohmi Rohmi Rohmi Rohmi Rohmi Rohmi Rohmi Rohmi, Rohmi Siti Karyani Siti Zaetun Siti Zaetun Siti Zaetun Siti Zaetun, Siti Sri Hartuti Sudirman Halla Sufina Zikria Putri Supriyanti, Ispi Thomas Tandi Manu Vivi Oktapia Wahyuni, Leni Waisnawa, I Made Wahyu Widyastini, Ni Komang Ari Santi Wulandari, Ayu Nurislami Yudha Anggit Jiwantoro Yuliana Tri Risky Yunan Jiwintarum Zahira, Jauhara Rana