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Karakterisasi Kromosom Sel Heterohibrida Dengan Teknik Karyotiping I Ketut Berata
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2647

Abstract

The aim of the research was to characterize the chromosomal structure of the heterohybride cells by karyotyping technique. Heterohybride cells were made by fusion of Bali cattle lymphocyte cells with mouse myelomma in selective medium which contains hypoxanthin, aminopterin and thymidine. Those cells which showed growth then were identified, selected and isolated for characterizing its chromosomal structure by karyotyping technique. The result showed that all of the chromosomal structure of the heterohybride cells were diploidy. The average of diploidy chromosome of heterohybride cells were 97 diploid Bali cattle lymphocyte cells and myelomma of mouse. The conclusion of the research is the heterohybride cells do not contain abnormal chromosome and are most potent to develop as candidate of Jembrana diseases vaccine.
ASPEK PATOLOGIS INFEKSI PARVOVIRUS PADA ANAK ANJING DI KOTA DENPASAR Ida Bagus Oka Winaya; I Ketut Berata; AAA Mirah Adi; I Made Kardena
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.083 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2619

Abstract

Telah dilakukan penelitian mengenai aspek patologis infeksi parvovirus pada anak anjing di Denpasar. Sebanyak 80 ekor anak anjing telah diperiksa pada Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Udayana periode tahun 2011- 2012. Enam belas ekor diantaranya menunjukkan gejala klinis berak darah, depresi, anoreksia, dan dehidrasi. Sinyalemen mengenai umur, jenis kelamin, dan ras juga dicatat. Perubahan patologi anatomi secara signifikan berupa enteritis haemorrhagis et necrotican dapat ditemukan pada semua anak anjing penderita. Kongesti dan nekrosis ditemukan pada epikardium. Gambaran yang bersifat anemia ditemukan pada jaringan limpa, ginjal, hati, sedangkan paruparu mengalami hiperemia. Secara mikroskopis, pada usus halus mengalami hiperemia disertai dengan infiltrasi limfosit, villi terlihat atropi dan nekrosis pada kripta Lieberkuhn. Nekrosis limfosit (limfositolisis) ditemukan pada folikel limpa. Kongesti dan nekrosis pada otot jantung namun intranuclear inclusion bodies hanya ditemukan pada satu anak anjing penderita. Penebalan ditemukan pada septa alveoli sedangkan pada hati dan ginjal hanya ditemukan peradangan ringan. Secara klinis bentuk enteritis hemorhagis et necrotican selalu ditemukan pada anak anjing terinfeksi parvovirus di Kota Denpasar. ________________________
PERUBAHAN HISTOLOGIS DAN RESPONS IMUNITAS SAPI BALI YANG DIBERIKAN PAKAN CAMPURAN KONSENTRAT I ketut Berata; Ida Bagus Oka Winaya; I Made Kardena
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.275 KB) | DOI: 10.21157/j.ked.hewan.v6i2.307

Abstract

Penelitian ini bertujuan mengetahui perubahan histologis dan respons kekebalan sapi bali yang diberikan pakan campuran konsentrat. Sebanyak 12 ekor sapi bali betina berumur 2 tahun, dibagi secara acak atas 3 kelompok perlakuan yaitu kelompok I diberi pakan rumput, kelompok II diberi pakan campuran 2 bagian rumput dan 1 bagian konsentrat, dan kelompok III diberi pakan campuran 1 bagian rumput dan 1 bagian konsentrat. Sebelum diberi perlakuan, dilakukan uji respons kekebalan seluler dengan teknik uji methylthiazol tetrazolium (MTT). Uji respons kekebalan dilakukan kembali pada bulan ke-3 dan sesaat sebelum dilakukan nekropsi. Pada bulan ke-10 dilakukan nekrosi terhadap 2 ekor sapi dari masing-masing kelompok perlakuan. Sisa sapi dari masing-masing kelompok perlakuan dilanjutkan diberi perlakuan untuk penelitian lanjutan. Sapi yang dinekropsi diambil jaringan pencernaan yaitu usus, untuk selanjutnya diproses dalam pembuatan sediaan histologis. Sediaan histologis diwarnai dengan hematoksilin eosin (HE). Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan struktur histologis usus antara ketiga kelompok perlakuan sedangkan respons kekebalan seluler tertinggi pada kelompok yang diberi pakan konsentrat yang lebih banyak.
Patological Changes in Liver and Gall Bladder Of Bali Cattle Infected by Fasciolosis I Made Kardena; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; Ida Bagus Windia Adnyana; I Made Sukada; Kadek Karang Agustina; Putu Agus Trisna Kusuma Antara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p02

Abstract

Fascioliosis is a parasitic disease that infects ruminants and the disease is widely spread in the world. Fascioliosis caused by Fasciola hepatica and Fasciola gigantica that can cause macroscopic and microscopic lesions in the liver and gall bladder of bali cattle. Samples of bali cattle in Pesanggaran slaughter house that infected with fasciolosis were used in this study. The pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The parasite found in the liver and gall bladder, thickening of bile duct mucous were observed on macroscopic examination. However, in microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy and hyperplasia of the bladder epithelium were occurred.
UMUR SAPI BALI BERPENGARUH PADA RESPON KEKEBALAN SELULER TERHADAP VIRUS PENYAKIT JEMBRANA PASCA VAKSINASI BERATA, I KETUT
Majalah Ilmiah Peternakan Vol 12 No 3 (2009)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.282 KB)

Abstract

ABSTRAK Penelitian bertujuan untuk mempelajari umur sapi Bali yang terbaik untuk divaksinasi agar respon kekebalan selulernya optimal terhadap virus penyakit Jembrana. Penelitian ini menggunakan 3 ekor sapi Bali betina yang berumur 7, 9 dan 12 bulan. Masing-masing sapi diadaptasikan 2 minggu dan diberikan obat cacing. Pemberian pakan 3 kali sehari dan air minum secara ad libitum. Vaksinasi dilakukan dengan vaksin JD.Vacc.sp.15 (produksi BBVet Denpasar) pada masing-masing sapi sebanyak 3 ml secara intramuskuler, satu bulan kemudian dilakukan booster dengan dosis dan rute yang sama. Seminggu pasca vaksinasi booster, darah perifernya diambil untuk mengisolasi limfosit dengan teknik picoll-paque gradient. Limfosit dikultur dalam media DMEM tanpa serum untuk selanjutnya dilakukan uji MTT. Uji MTT digunakan untuk menguji respon kekebalan seluler. Mitogen yang digunakan adalah protein virus Jembrana. Hasil penelitian menunjukkan adanya perbedaan respon kekebalan seluler yang bermakna (p<0,05) antara sapi umur 7 bulan dengan 9 dan 12 bulan. Tidak terdapat perbedaan respon kekebalan seluler yang bermakna (p>0,05) antara sapi umur 9 bulan dengan 12 bulan. Hasil ini menunjukkan bahwa umur sapi 12 bulan merupakan umur tepat mulai diberikan vaksinasi, karena respon kekebalan selulernya optimal. AGE OF BALI CATTLE IS TO INFLUENCE ON THE CELLULAR IMMUNE RESPONSE AGAINST JEMBRANA DISEASE VIRUS POST VACCINATION ABSTRACT The aim of this research is to study the accuratly age of Bali cattle vaccination so that could obtained the optimal cellular immune response against Jembrana virus disease. In this research was used three Bali cattles i.e. 7, 9 and 12 month of age respectively. Each of the cattles were adapted in two weeks and were given the anthelmintic treatment. The feeding was given three times daily and the drinking water ad libitum. The vaccine was inoculated by 3 ml JD.vacc.sp.15 vaccine (BBVet Denpasar production) by intramuscular route respectively. One month after first vaccination, was conducted booster by similarly dose and route respectively. One week after the booster, from the each cattle was taken its peripheral blood cells for to isolated their lymphocytes by picoll-paque gradient method. Each of this lymphocyte cells were cultured in non serum DMEM media for the MTT assay. MTT assay was used for to determine the cellular immune response. The mitogen was used whole protein of Jembrana disease virus. Result of the research showed significantly difference (p<0,05) of cellular immune response among the age 7 month with 9 and 12 month of cattles. There were not significantly difference (p>0,05) of the response between 9 with 12 month of age. This result indicated that the cattle which 9 month of age is the good timing to start the vaccination, because its cellular immune response is optimal.
Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models Marson, Fransiska Gratia Sonita; Sewoyo, Palagan Senopati; Astawa, I Nyoman Mantik; Adi, Anak Agung Ayu Mirah; Suardana, Ida Bagus Kade; Winaya, Ida Bagus Oka; Berata, I Ketut
Media Kedokteran Hewan Vol. 36 No. 1 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i1.2025.13-20

Abstract

c-Jun is often found to be overexpressed in various cancers, so this gene might be a target for cancer therapy. Newcastle disease virus (NDV) is recognized for its oncolytic properties and potential as a cancer virotherapy agent, with various mechanisms reported to trigger cancer cell death. This study aimed to assess the c-Jun expression in rat mammary cancer models. Rat mammary cancer models were categorized into two treatment groups: the control group (C) and the virotherapy group (V). Group C was administered with 0.5 cc of sterile PBS, while group V received 7 log 2 HAU per 0.5 cc of the Indonesian NDV field isolate Tabanan-1/ARP/2017 intratumorally. The treatment was carried out for four days in a row. Two weeks after treatment, all rats were humanely euthanized, and mammary cancer tissues were excised for further examination. Mammary cancer tissues were examined histopathologically and analyzed using immunohistochemistry to determine intranuclear c-Jun expression, quantified by the H-Score. The results demonstrated that NDV significantly reduced c-Jun expression. It can be inferred that NDV Tabanan-1/ARP/2017 holds potential as a mammary cancer therapy agent by reducing c-Jun expression. This finding is considered novel, as there have been no previous reports of decreased c-Jun expression following virotherapy with NDV.
Identification of Colisepticemia in Broiler Chicken in Tabanan, Bali Devira, Dinda; Ketut Tono Pasek Gelgel; I Ketut Berata
Jurnal Veteriner dan Biomedis Vol. 3 No. 1 (2025): Maret
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.3.1.1-8.

Abstract

Colisepticemia is a condition where Escherichia coli and its toxins have spread to tissues or organs that can cause organ damage or inflammation. Colisepticemia is a sign of acute colibacillosis in poultry which can result in death and in its subacute form is characterized by pericarditis, airsacculitis and perihepatitis. The incidence of colibacillosis in poultry can result in economic losses due to reduced production, increased medical costs, and even death. The aim of this study was to establish a diagnosis of the death of 15 days old broiler chicken. Based on the necropsy results, characteristic anatomical pathology was found in the form of fibrin membranes on the heart and liver, as well as lesions on other organs. Identification and isolation of bacteria in the laboratory using samples from the heart, liver and intestines showed positive results for Escherichia coli. Parasitological examination using feces did not reveal any parasitic agents involved in this case. Based on clinical symptoms, anatomical pathology examination, histopathology, culture and identification of bacteria, it can be concluded that the cause of chicken death was colisepticemia caused by Avian Pathogenic Escherichia Coli (APEC).
Coccidiosis In Broiler Chicken Aged 27 Days From A Farm In Tunjuk, Marga, Tabanan, Bali: A Case Study Amelia, Ni Kadek Shita; Suratma, Nyoman Adi; Berata, I Ketut; Besung, I Nengah Kerta; Mahardika, I Gusti Ngurah Kade
Jurnal Medika Veterinaria Vol 19, No 2 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i2.48001

Abstract

Coccidiosis, also commonly referred to as bloody diarrhea, is caused by Eimeria spp. and primarily affects the digestive tract. The most pathogenic Eimeria species in chickens are Eimeria tenella and Eimeria necatrix. The subject of this case study was a 27-day-old female broiler chicken from a commercial farm located in Tunjuk Village, Marga Subdistrict, Tabanan Regency, Bali. The chicken had been reported to suffer from bloody diarrhea for five days, accompanied by clinical signs of weakness, anorexia, pale comb, ruffled feathers, and a tendency to huddle in the corner of the pen. This case study aimed to describe the anatomical pathology, histopathology, and laboratory findings to obtain a definitive diagnosis of the disease affecting this chicken. The results of the anatomical pathology examination showed hemorrhage in the cecum. Histopathological examination of the cecum revealed the presence of schizonts, hemorrhage, and inflammatory cell infiltration. Qualitative fecal examination confirmed the presence of Eimeria spp. oocysts, while quantitative fecal examination using the McMaster technique revealed 895,600 oocysts per gram of feces, indicating a severe infection. Based on clinical observations, epidemiological data, gross pathological changes, histopathological findings, and fecal examinations using native, sedimentation, flotation, and McMaster methods, it was concluded that the chicken was affected by coccidiosis.
PERUBAHAN HISTOLOGIS DAN RESPONS IMUNITAS SAPI BALI YANG DIBERIKAN PAKAN CAMPURAN KONSENTRAT Berata, I ketut; Winaya, Ida Bagus Oka; Kardena, I Made
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.307

Abstract

Penelitian ini bertujuan mengetahui perubahan histologis dan respons kekebalan sapi bali yang diberikan pakan campuran konsentrat. Sebanyak 12 ekor sapi bali betina berumur 2 tahun, dibagi secara acak atas 3 kelompok perlakuan yaitu kelompok I diberi pakan rumput, kelompok II diberi pakan campuran 2 bagian rumput dan 1 bagian konsentrat, dan kelompok III diberi pakan campuran 1 bagian rumput dan 1 bagian konsentrat. Sebelum diberi perlakuan, dilakukan uji respons kekebalan seluler dengan teknik uji methylthiazol tetrazolium (MTT). Uji respons kekebalan dilakukan kembali pada bulan ke-3 dan sesaat sebelum dilakukan nekropsi. Pada bulan ke-10 dilakukan nekrosi terhadap 2 ekor sapi dari masing-masing kelompok perlakuan. Sisa sapi dari masing-masing kelompok perlakuan dilanjutkan diberi perlakuan untuk penelitian lanjutan. Sapi yang dinekropsi diambil jaringan pencernaan yaitu usus, untuk selanjutnya diproses dalam pembuatan sediaan histologis. Sediaan histologis diwarnai dengan hematoksilin eosin (HE). Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan struktur histologis usus antara ketiga kelompok perlakuan sedangkan respons kekebalan seluler tertinggi pada kelompok yang diberi pakan konsentrat yang lebih banyak.
CANINE PARVOVIRUS AND ESCHERICHIA COLI IN INFECTION MIX BREED DOG Made Ramadhinita Desrianti; Gusti Ayu Yuniati Kencana; I Gusti Ketut Suarjana; I Ketut Berata; I Made Dwinata
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2024 Vol. 16 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i05.p09

Abstract

Canine Parvovirus Disease is an infectious disease that causes death in dogs by Canine Parvovirus (CPV). This disease is fatal and contagious, causing the highest number of deaths in dogs, especially in puppies under six months of age. The aim of this research is to find out how to diagnose diseases caused by Canine parvovirus and other secondary infections. Diagnosis is made by looking at clinical signs, anatomical pathology and histopathology and carrying out a PCR test. A 3 month old male mixed breed dog with clinical signs of weakness, lack of appetite and drinking, vomiting and bloody diarrhea. The anatomical pathological changes observed were brain congestion, the heart apex was blunt, the liver and lungs were hemorrhagic, the spleen was dark red in some parts, and the intestine was hemorrhagic. Histopathologically, changes were found in the animal's organs, heart, lungs, liver, spleen and intestines. Virus test samples from intestines, spleen and heart. The results of the Polymerase Chain Reaction (PCR) test showed a positive result with a length of 900 bp. The bacterial test results from the lung organ samples were positive for Escherichia coli which was marked red on Mc Conkey media. It was concluded that the dog was infected with Canine Parvovirus and Escherichia Coli.
Co-Authors Afrizal Choirul Umam Agnes Endang Tri H Aida Lousie Tenden Rompis Ainul - Hidayah Alviana Rizqiyah Utami Amelia, Ni Kadek Shita Anak Agung Ayu Mirah Adi Anak Agung Gde Arjana Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Anak Agung Gde Jaya Warditha Anak Agung Oka Anak Agung Sagung Kendran Anak Agung Sagung Kendran Apsari, Ni Luh Putu Nadia Ardi Sandriya Aristawati, I Dewa Agung Ayu Irma Astari, Ni Putu Widya Astini, Ni Putu Sri Ayu Aulia Insani, Aulia Ayu Prawitasari Citra Pratama Bina Ichsantya Budiartawan, I Komang Alit Damara, Doni Darmawan, I Gusti Ayu Chintya Darmayanti, Mahda Dwi Delima Marsinta Ida Pasaribu Devira, Dinda Dewa Ayu Dwita Karmi Dhinar Wahyu Prasetyo Dini Hilary Manullang DORTY PRIHASTINA SALBAHAGA Duwiri, Christine Valeri Dwi Muslifah Nur Hijayani DWI SURYANTO Dzikri Nurma'rifah Takariyanti Elisabeth Karina Elsa Hidayati Elti Febilani Elyda . Erwanti Siti Rabiah, Erwanti Siti Farhan Abdul Hasan, Farhan Abdul Febe Adonia Renandra Hermawan Febrianty, Ni Made Dhea Fitri Irawan Rahmawandani Franky Samuel Milenyano Chandra Gde Jasmara Muda Gheasella Fia Gunawan Ginting, Regina Bonifasia Br Gunawan, Stefanus Andre Gunawati, Luh Sri Guru, Yohana Cendyka Kartika Dewi Gusti Agung Ayu Alecia Putri Anugrahaeni Gusti Agung Ayu Putu Adriyati, Gusti Agung Ayu Gusti Ayu Yuniati Kencana Hamong Suharsono Hapsari Mahatmi Hendrina Konda M Meha, Hendrina Konda M Humaira, Sarah Husnul Khatimah I Dewa Made Adhiwitana I GEDE ENDRA KUSUMA I Gusti Bagus Sathya Dharma, I Gusti Bagus Sathya I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Gusti Putu Tovan Mahottama I KETUT ELI SUPARTIKA I Ketut Eli Supartika I Ketut Eli Supartika I Ketut Puja I Ketut Suatha I Ketut Suatha I Ketut Sumadi I Ketut Wirata I Made Damriyasa I Made Dwinata I Made Dwinata I Made Indrayadnya Swarayana I Made Kardena I Made Kardena I Made Merdana I Made Merdana I Made Merdana I Made Putra Wiadnyana I Made Sukada I N. T. ARIANA I Nengah Kerta Besung I NYOMAN ADI SURATMA I NYOMAN MANTIK ASTAWA I Nyoman Mantik Astawa I Nyoman Sulabda I Putu Suparman I W BUDIARSA SUYASA I Wayan Gorda I Wayan Sudira I Wayan Sudira I Wayan Wirata I Wayan Wirata Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Made Oka Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Iwan Harjono Utama Janardani, Ni Made Kunti Jayawardhita, Anak Agung Gde Kadek Karang Agustina Kadek Karina Dewi Wijayanthi Kartika, Erena Hajar Ketut Budiasa Ketut Eli Supartika Ketut Novi Kusmayani Ketut Tono Pasek Gelgel Kevin Dominika Kristi Agusti Putri, Kristi Agusti Kusuma, Putu Winatha Laila Gianita Veralyn Luh Gde Sri Surya Heryani Luh Made Sudimartini Luh Made Sudimartini Luh Made Sudimartini Luh Putu Syamadina Pramesya Nareswari Lusiana Flora Ndagu Made Oka Adinata Made Rahayu Kusumadewi Made Ramadhinita Desrianti Made Sandy Widhi Raharja Marissa Divia Dayanti Marson, Fransiska Gratia Sonita MAS DJOKO RUDYANTO Muhamad Furkam Fadilah Muhsi, Ach Moh Abd Nesia Masniari Helena Sibarani Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Made Ayu Suastami Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Nofantri, Lidia Noviriolla Maria Nugraha, Putri Nurhasyiat Nasaruddin Nyoman Adi Suratma Oktaviandari, Putu Risma Palagan Senopati Sewoyo Pangesti, Thiara A. Pramesti, Ni Komang Lady Prista Oktafebri Yulestari Pujaswarini, Ni Made Hani Purnama, Komang Andika Purnata, I Dewa Nyoman Alit Putra, I Putu Agus Antara Putri Yuliana Mangindaan Putu Agus Trisna Kusuma Antara Putu Henrywaesa Sudipa Putu Suastika Raharjo, Yudha Yaksa Crada Yoga Arum Rahmat Grahadi RAHMI MUSTABA Sam suri Samsuri Samsuri Samsuri Samsuri Sari Sartini, Sari Setianingsih, Ni Luh Eka Setyawati, Luh Gede Sewoyo, Palagan Senopati Sihotang, Tanti Fitri Sri Kayati Widyastuti Suranjaya I .Gd Tanuwijaya, Phebe Amadea Vaswani Samaria Napitupulu Wahyu Semadi Putra Waskitha, Melati Pusparini Widia Insani Wulandari, Meidi Andira Yanne Yanse Rumlaklak Yesi Veronica Sitepu Yustisia, Anggia Yuyun Setia Ningsih Zumara Mufida Hidayati