Articles
Identifikasi Sel-sel Target Virus Penyakit Jembrana dengan Teknik Imunositokimia Ganda
I Ketut Berata;
I Nyoman Mantik Astawa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta
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DOI: 10.24002/biota.v16i2.105
Penelitian bertujuan untuk mengidentifikasi sel target virus Jembrana dengan teknik imunositokimia ganda. Penelitian ini menggunakan sapi Bali yang diinokulasi dengan virus Jembrana secara intramuskuler. Pada demam hari kedua setelah inokulasi virus, sapi dinekropsi. Limpa diambil secara aseptik, kemudian direndam dalam buffer formalin 10% selama 24 jam. Potongan limpa diproses untuk pembuatan sediaan histologis dengan menggunakan cryomicrotome. Preparat histologis limpa diwarnai dengan teknik imunositokimia ganda. Untuk mengidentifikasi subset limfosit digunakan antibody monoclonal anti BoCD4 + dan anti BoCD8 + , serta diamino benzidine (DAB) sebagai substrat. Pada pewarnaan ini, sel-sel terinfeksi akan tampak berwarna biru, sedangkan sel-sel marka BoCD4 + atau BoCD8 + akan tampak berwarna coklat. Untuk identifikasi sel-sel terinfeksi virus Jembrana digunakan antibodi monoklonal anti-capsid JDV (BB-Vet Denpasar) dan nitro blue tetrazolium (NBT) sebagai substrat. Hasil penelitian menunjukkan bahwa sel-sel yang terinfeksi virus Jembrana hanya bermarka BoCD4 + , dan sama sekali tidak pada sel BoCD8 + . Simpulan penelitian ini adalah sel-sel BoCD4 + merupakan sel target virus Jembrana.
Karakterisasi Kromosom Sel Heterohibrida Dengan Teknik Karyotiping
I Ketut Berata
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta
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DOI: 10.24002/biota.v15i1.2647
The aim of the research was to characterize the chromosomal structure of the heterohybride cells by karyotyping technique. Heterohybride cells were made by fusion of Bali cattle lymphocyte cells with mouse myelomma in selective medium which contains hypoxanthin, aminopterin and thymidine. Those cells which showed growth then were identified, selected and isolated for characterizing its chromosomal structure by karyotyping technique. The result showed that all of the chromosomal structure of the heterohybride cells were diploidy. The average of diploidy chromosome of heterohybride cells were 97 diploid Bali cattle lymphocyte cells and myelomma of mouse. The conclusion of the research is the heterohybride cells do not contain abnormal chromosome and are most potent to develop as candidate of Jembrana diseases vaccine.
Pelacakan Secara Imunohistokimiawi Antigen Ekskretori-Sekretori pada Sapi Bali yang Terinfeksi Fasciola gigantica (IMMUNOHISTOCHEMICAL DETECTION OF EXCRET0RY-SECRETORY ANTIGENS IN BALI CATLLE INFECTED BY FASCIOLA GIGANTICA)
Ida Bagus Oka Winaya;
I Nyoman Mantik Astawa;
I Made Damriyasa;
Nyoman Sadra Dharmawan;
I Ketut Berata
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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In order to study the distribution of excretory-secretory (ES) F. gigantica in liver tissue of infected balicattle a research was establisihed using monoclonal antibodies (mAbs) againts ES antigens. Immortalmouse myeloma cells were fused with the lymphocytes derived from the spleen of mice that immunizedwith ES antigen. The mAbs were tested for their specificity by using enzyme linked immunosorbent assay(ELISA). Five specific mAbs againts ES antigens were isolated and two mAbs were used for immunodetectionof ES antigens in liver tissue of bali cattle. Immunohistochemical ES antigens were not detected in paraffinembeded tissue of negative confirmed fasciolosis samples. ES antigens was detected in hepatocytes andcytoplasm of bile duct epithelims in the bali cattle that infected with fasciolosis in moderate intensity.Therfore indicated that mAbs produced in this study are applicable for detecting ES antigens in bali cattleinfected by F. gigantica.
Dermatitis Suppurative Mengikuti Infeksi Tungau Demodeks dan Sarcoptes pada Anjing Kampung di Jalanan Kota Denpasar
Ida Bagus Oka Winaya;
Ida Bagus Windia Adnyana;
I Ketut Berata;
Ida Ayu Pasti Apsari
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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DOI: 10.19087/jveteriner.2022.23.3.328
Tujuan penelitian ini adalah untuk mengetahui penyebab dermatitis dan kerontokan rambut pada anjing jalanan di Kota Denpasar. Dilakukan pencatatan terhadap identitas 75 ekor anjing yang meliputi umur, ras, tipe rambut dan lokasi lesi pada permukaan tubuh. Sampel biopsi kulit dengan ukuran 6 mm diambil dari lesi kulit yang paling parah dengan tanda klinis hiperemia, gatal, adanya krusta, bersisik/scaling, kebotakan/alopecia. Pengambilan sampel biopsi dilakukan dengan terlebih dulu memberikan injeksi anestetik lokal dan dilanjutkan dengan pemberian spraying antiseptik setelah pengangkatan jaringan biopsi. Sampel jaringan kulit kemudian difiksasi dalam larutan neutral buffered formalin 10%. Setelah 24-48 jam dalam cairan NBF jaringan diembedding dalam blok paraffin dengan metode standar. Sedian dengan ketebalan 5 mikron selanjutnya diwarnai dengan hematoksilin-eosin (HE). Pemeriksaan histopatologi terhadap tujuh ekor anjing penderita dermatitis dan kerontokan rambut umur di bawah satu tahun ditemukan kumpulan tungau demodek di beberapa folikel bulu. Eksudat radang netrofil dan eritrosit juga ditemukan pada permukaan epidermis yang nekrosis. Pada pemeriksaan terhadap 26 ekor anjing penderita dermatitis dan kerontokan bulu umur di atas satu tahun ditemukan 24 ekor anjing murni terinfeksi tungau demodeks dan dua ekor terinfeksi tungau demodeks dan sarcoptes. Ditemukan juga adanya larva tungau demodeks di luar folikel rambut. Larva tungau perifolikel terlihat dikelilingi oleh netrofil pada area dermis nekrosis, sedangkan pada anjing yang terinfeksi oleh dua jenis tungau hanya ditemukan respons radang ringan di sekitar folikel rambut.
ASPEK PATOLOGIS INFEKSI PARVOVIRUS PADA ANAK ANJING DI KOTA DENPASAR
Ida Bagus Oka Winaya;
I Ketut Berata;
AAA Mirah Adi;
I Made Kardena
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v8i2.2619
Telah dilakukan penelitian mengenai aspek patologis infeksi parvovirus pada anak anjing di Denpasar. Sebanyak 80 ekor anak anjing telah diperiksa pada Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Udayana periode tahun 2011- 2012. Enam belas ekor diantaranya menunjukkan gejala klinis berak darah, depresi, anoreksia, dan dehidrasi. Sinyalemen mengenai umur, jenis kelamin, dan ras juga dicatat. Perubahan patologi anatomi secara signifikan berupa enteritis haemorrhagis et necrotican dapat ditemukan pada semua anak anjing penderita. Kongesti dan nekrosis ditemukan pada epikardium. Gambaran yang bersifat anemia ditemukan pada jaringan limpa, ginjal, hati, sedangkan paruparu mengalami hiperemia. Secara mikroskopis, pada usus halus mengalami hiperemia disertai dengan infiltrasi limfosit, villi terlihat atropi dan nekrosis pada kripta Lieberkuhn. Nekrosis limfosit (limfositolisis) ditemukan pada folikel limpa. Kongesti dan nekrosis pada otot jantung namun intranuclear inclusion bodies hanya ditemukan pada satu anak anjing penderita. Penebalan ditemukan pada septa alveoli sedangkan pada hati dan ginjal hanya ditemukan peradangan ringan. Secara klinis bentuk enteritis hemorhagis et necrotican selalu ditemukan pada anak anjing terinfeksi parvovirus di Kota Denpasar. ________________________
PERUBAHAN HISTOLOGIS DAN RESPONS IMUNITAS SAPI BALI YANG DIBERIKAN PAKAN CAMPURAN KONSENTRAT
I ketut Berata;
Ida Bagus Oka Winaya;
I Made Kardena
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala
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DOI: 10.21157/j.ked.hewan.v6i2.307
Penelitian ini bertujuan mengetahui perubahan histologis dan respons kekebalan sapi bali yang diberikan pakan campuran konsentrat. Sebanyak 12 ekor sapi bali betina berumur 2 tahun, dibagi secara acak atas 3 kelompok perlakuan yaitu kelompok I diberi pakan rumput, kelompok II diberi pakan campuran 2 bagian rumput dan 1 bagian konsentrat, dan kelompok III diberi pakan campuran 1 bagian rumput dan 1 bagian konsentrat. Sebelum diberi perlakuan, dilakukan uji respons kekebalan seluler dengan teknik uji methylthiazol tetrazolium (MTT). Uji respons kekebalan dilakukan kembali pada bulan ke-3 dan sesaat sebelum dilakukan nekropsi. Pada bulan ke-10 dilakukan nekrosi terhadap 2 ekor sapi dari masing-masing kelompok perlakuan. Sisa sapi dari masing-masing kelompok perlakuan dilanjutkan diberi perlakuan untuk penelitian lanjutan. Sapi yang dinekropsi diambil jaringan pencernaan yaitu usus, untuk selanjutnya diproses dalam pembuatan sediaan histologis. Sediaan histologis diwarnai dengan hematoksilin eosin (HE). Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan struktur histologis usus antara ketiga kelompok perlakuan sedangkan respons kekebalan seluler tertinggi pada kelompok yang diberi pakan konsentrat yang lebih banyak.
Patological Changes in Liver and Gall Bladder Of Bali Cattle Infected by Fasciolosis
I Made Kardena;
Ida Bagus Oka Winaya;
Anak Agung Ayu Mirah Adi;
I Ketut Berata;
Ida Bagus Windia Adnyana;
I Made Sukada;
Kadek Karang Agustina;
Putu Agus Trisna Kusuma Antara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University
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DOI: 10.24843/JVAS.2017.v01.i01.p02
Fascioliosis is a parasitic disease that infects ruminants and the disease is widely spread in the world. Fascioliosis caused by Fasciola hepatica and Fasciola gigantica that can cause macroscopic and microscopic lesions in the liver and gall bladder of bali cattle. Samples of bali cattle in Pesanggaran slaughter house that infected with fasciolosis were used in this study. The pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The parasite found in the liver and gall bladder, thickening of bile duct mucous were observed on macroscopic examination. However, in microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy and hyperplasia of the bladder epithelium were occurred.
UMUR SAPI BALI BERPENGARUH PADA RESPON KEKEBALAN SELULER TERHADAP VIRUS PENYAKIT JEMBRANA PASCA VAKSINASI
BERATA, I KETUT
Majalah Ilmiah Peternakan Vol 12 No 3 (2009)
Publisher : Fakultas Peternakan Universitas Udayana
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ABSTRAK Penelitian bertujuan untuk mempelajari umur sapi Bali yang terbaik untuk divaksinasi agar respon kekebalan selulernya optimal terhadap virus penyakit Jembrana. Penelitian ini menggunakan 3 ekor sapi Bali betina yang berumur 7, 9 dan 12 bulan. Masing-masing sapi diadaptasikan 2 minggu dan diberikan obat cacing. Pemberian pakan 3 kali sehari dan air minum secara ad libitum. Vaksinasi dilakukan dengan vaksin JD.Vacc.sp.15 (produksi BBVet Denpasar) pada masing-masing sapi sebanyak 3 ml secara intramuskuler, satu bulan kemudian dilakukan booster dengan dosis dan rute yang sama. Seminggu pasca vaksinasi booster, darah perifernya diambil untuk mengisolasi limfosit dengan teknik picoll-paque gradient. Limfosit dikultur dalam media DMEM tanpa serum untuk selanjutnya dilakukan uji MTT. Uji MTT digunakan untuk menguji respon kekebalan seluler. Mitogen yang digunakan adalah protein virus Jembrana. Hasil penelitian menunjukkan adanya perbedaan respon kekebalan seluler yang bermakna (p<0,05) antara sapi umur 7 bulan dengan 9 dan 12 bulan. Tidak terdapat perbedaan respon kekebalan seluler yang bermakna (p>0,05) antara sapi umur 9 bulan dengan 12 bulan. Hasil ini menunjukkan bahwa umur sapi 12 bulan merupakan umur tepat mulai diberikan vaksinasi, karena respon kekebalan selulernya optimal. AGE OF BALI CATTLE IS TO INFLUENCE ON THE CELLULAR IMMUNE RESPONSE AGAINST JEMBRANA DISEASE VIRUS POST VACCINATION ABSTRACT The aim of this research is to study the accuratly age of Bali cattle vaccination so that could obtained the optimal cellular immune response against Jembrana virus disease. In this research was used three Bali cattles i.e. 7, 9 and 12 month of age respectively. Each of the cattles were adapted in two weeks and were given the anthelmintic treatment. The feeding was given three times daily and the drinking water ad libitum. The vaccine was inoculated by 3 ml JD.vacc.sp.15 vaccine (BBVet Denpasar production) by intramuscular route respectively. One month after first vaccination, was conducted booster by similarly dose and route respectively. One week after the booster, from the each cattle was taken its peripheral blood cells for to isolated their lymphocytes by picoll-paque gradient method. Each of this lymphocyte cells were cultured in non serum DMEM media for the MTT assay. MTT assay was used for to determine the cellular immune response. The mitogen was used whole protein of Jembrana disease virus. Result of the research showed significantly difference (p<0,05) of cellular immune response among the age 7 month with 9 and 12 month of cattles. There were not significantly difference (p>0,05) of the response between 9 with 12 month of age. This result indicated that the cattle which 9 month of age is the good timing to start the vaccination, because its cellular immune response is optimal.
Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models
Marson, Fransiska Gratia Sonita;
Sewoyo, Palagan Senopati;
Astawa, I Nyoman Mantik;
Adi, Anak Agung Ayu Mirah;
Suardana, Ida Bagus Kade;
Winaya, Ida Bagus Oka;
Berata, I Ketut
Media Kedokteran Hewan Vol. 36 No. 1 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga
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DOI: 10.20473/mkh.v36i1.2025.13-20
c-Jun is often found to be overexpressed in various cancers, so this gene might be a target for cancer therapy. Newcastle disease virus (NDV) is recognized for its oncolytic properties and potential as a cancer virotherapy agent, with various mechanisms reported to trigger cancer cell death. This study aimed to assess the c-Jun expression in rat mammary cancer models. Rat mammary cancer models were categorized into two treatment groups: the control group (C) and the virotherapy group (V). Group C was administered with 0.5 cc of sterile PBS, while group V received 7 log 2 HAU per 0.5 cc of the Indonesian NDV field isolate Tabanan-1/ARP/2017 intratumorally. The treatment was carried out for four days in a row. Two weeks after treatment, all rats were humanely euthanized, and mammary cancer tissues were excised for further examination. Mammary cancer tissues were examined histopathologically and analyzed using immunohistochemistry to determine intranuclear c-Jun expression, quantified by the H-Score. The results demonstrated that NDV significantly reduced c-Jun expression. It can be inferred that NDV Tabanan-1/ARP/2017 holds potential as a mammary cancer therapy agent by reducing c-Jun expression. This finding is considered novel, as there have been no previous reports of decreased c-Jun expression following virotherapy with NDV.
Identification of Colisepticemia in Broiler Chicken in Tabanan, Bali
Devira, Dinda;
Ketut Tono Pasek Gelgel;
I Ketut Berata
Jurnal Veteriner dan Biomedis Vol. 3 No. 1 (2025): Maret
Publisher : Sekolah Kedokteran Hewan dan Biomedis
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DOI: 10.29244/jvetbiomed.3.1.1-8.
Colisepticemia is a condition where Escherichia coli and its toxins have spread to tissues or organs that can cause organ damage or inflammation. Colisepticemia is a sign of acute colibacillosis in poultry which can result in death and in its subacute form is characterized by pericarditis, airsacculitis and perihepatitis. The incidence of colibacillosis in poultry can result in economic losses due to reduced production, increased medical costs, and even death. The aim of this study was to establish a diagnosis of the death of 15 days old broiler chicken. Based on the necropsy results, characteristic anatomical pathology was found in the form of fibrin membranes on the heart and liver, as well as lesions on other organs. Identification and isolation of bacteria in the laboratory using samples from the heart, liver and intestines showed positive results for Escherichia coli. Parasitological examination using feces did not reveal any parasitic agents involved in this case. Based on clinical symptoms, anatomical pathology examination, histopathology, culture and identification of bacteria, it can be concluded that the cause of chicken death was colisepticemia caused by Avian Pathogenic Escherichia Coli (APEC).