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All Journal Hemera Zoa Media Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Majalah Ilmiah Peternakan Jurnal Veteriner Indonesia Medicus Veterinus Ecotrophic, Journal of Environmental Science Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Media Kedokteran Hewan Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Journal of Veterinary and Animal Sciences Jurnal Medika Veterinaria

I Ketut Berata

Fakultas Kedokteran Hewan Universitas Udayana Bali

Author-ID : 242021

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Pengaruh Suplementasi Vitamin E terhadap Efek Samping Deksametason pada Paru-Paru Tikus Putih Jantan Bina Ichsantya; I Ketut Berata; Samsuri Samsuri; I Made Merdana
Buletin Veteriner Udayana Vol. 9 No. 2 Agustus 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi vitamin E sebagai antioksidan dalam mengurangi efek samping deksametason pada paru-paru. Penelitian ini menggunakan 25 ekor tikus putih jantan yang dibagi dalam lima kelompok perlakuan, yaitu: kelompok kontrol negatif yang diberi pakan dan minum saja, kelompok kontrol positif yang diberi deksametason secara subkutan dengan dosis 0,13 mg/kg bb, kelompok P1 yang diberi deksametason secara subkutan dengan dosis 0,13 mg/kg bb dan vitamin E per oral dengan dosis 100 mg/kg bb, kelompok P2 yang diberi deksametason secara subkutan dengan dosis 0,13 mg/kg bb dan vitamin E per oral dengan dosis 150 mg/kg bb dan kelompok P3 yang diberi deksametason secara subkutan dengan dosis 0,13 mg/kg bb dan vitamin E per oral dengan dosis 200 mg/kg. Setelah diberikan perlakuan selama 14 hari, kemudian dilakukan nekropsi,. Organ paru-paru diambil untuk diproses menjadi sediaan histopatologi dengan metode pewarnaan Hematoxylin-Eosin (HE). Variabel yang diperiksa meliputi edema, perdarahan dan peradangan. Hasil penelitian menunjukkan terdapat perubahan histopatologis pada jaringan paru yang berbeda nyata antara kelompok kontrol positif dan kelompok kontrol negatif, sedangkan perubahan kearah perbaikan pada jaringan paru tidak berbeda nyata pada seluruh kelompok P1, P2 dan P3. Simpulan dari penelitian ini adalah: pemberian deksametason dosis 0,13 mg/kg bb menyebabkan perubahan histopatologis pada jaringan paru tikus putih meliputi edema, perdarahan dan peradangan. Suplementasi vitamin E hingga dosis 200 mg/kg bb belum memberikan perbaikan yang signifikan pada jaringan paru tikus putih yang diberi deksametason.

Histopatologi Usus Halus Mencit Pasca Pemberian Ekstrak Etanol Daun Ashitaba (HISTOPHATOLOGICAL CHANGES ON SMALL INTESTINE OF MICE AGAINS WITH OF THE ETHANOL EXTRACT OF ASHITABA LEAVES) I Made Putra Wiadnyana; Ketut Budiasa; I Ketut Berata
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The purpose of this research was to know the effects of the ethanol leaf extract ashitaba(Angelica keiskei) against the histopathologicalchanges in the small intestine of mice (Musmusculus). This research used 15 mice (Mus musculus) with in average 25-30 gram and is divided into five group of treatment. Group A, were used as a control (placebo): groups B, C, D, and Eweregiven ashitaba leaf ethanol extract byorally in dose 125 mg/kg bw, 250 mg / kg bw, 500 mg / kgbw, and 1,000 mg/kg bw as long as 21 days respectively. On the 22nd days, all of the mice wasnecropsied and the small intestine tissues was took for the histopathological prepare processing.The results of histopathological examination of the small intestine of mice were fatty degenerationat one mouse in dose 125 mg/kg bw. On the mice by dose of 250 mg/kg bw of treatment, wereshowed fatty degeneration and inflammatory lesions. On the mice by dose 500 mg/kg bw, wereobtained that three of mice were affected fatty degeneration and inflammatory lesions. On the miceby dose 1,000 mg/kg bw were obtained that three of mice suffer inflammatory lesions. All of thetreatment of this research were not appeared necrose lesion. Conclussion of this research is thetreatment of the ethanol extract of ashitaba leaves by orally at a dose range from 125 mg/kg bw until dose 1,000 mg /kg bw as long as 21 days did not raise the significance histopathologicalchange in the small intestines of mice (Mus musculus).

Perubahan Histopatologi Hati Mencit (Mus musculus) yang Diberikan Ekstrak Daun Ashitaba (Angelica keiskei) I Made Indrayadnya Swarayana; I Wayan Sudira; I Ketut Berata
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Penelitian ini bertujuan untuk mengetahui perubahan histopatologi hati mencit (Musmusculus) yang diberikan ekstrak daun Ashitaba (Angelica keiskei). Penelitian inimenggunakan 25 ekor mencit jantan yang dibagi secara acak sederhana menjadi 5 grup.Grup A sebagai kontrol diberikan aquades, dan grup B, C, D, dan E masing-masingdiberikan 125 mg, 250 mg, 500 mg dan 1000 mg extrak Ashitaba secara oral. Pemberianekstrak etanol daun Ashitaba dilakukan setiap hari selama 21 hari. Pada hari ke 22 semuamencit dinekropsi dan hati diambil untuk diproses pembuatan preaparat dengan metodeembedding blocking dengan paraffin serta pewarnaan hematoxylin eosin (HE). Pemeriksaanperubahan histopatologi dilakukan berdasarkan adanya degenerasi melemak dan nekrosis.Hasil penelitian menunjukkan adanya nekrosis dan degenerasi yang ringan pada semuagrup perlakuan. Hasil analisis statistik dengan metode Kruskal Wallis menunjukkan hasilyang tidak berbeda diantara grup perlakuan. Penelitian ini membuktikan ekstrak daunAshitaba (Angelica keiskei) antara dosis 125 mg sampai 1.000 mg tidak menimbulkan efektoksik pada hati mencit.

Histopatologi Usus Halus Tikus Putih Jantan yang Diberikan Deksametason dan Vitamin E Kadek Karina Dewi Wijayanthi; I Ketut Berata; Samsuri Samsuri; I Wayan Sudira
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Dexamethasone it’s in period a synthetic corticosteroid drug that widely used by the public. If it used for long time and the use of large doses, causing oxidative stress in cells due to the accumulation of free radicals which may cause cell death in the body organs tissues. Vitamin E was known to have a good role as an antioxidant effect. Currently, unknown effects of dexamethasone and vitamin E administration on damage of the small intestine of rat (Rattus norvegicus). This study used an experimental design. Samples 25 male rats were divided into 5 groups, namely the negative control or no treatment (P0), positive control (P1) was given dexamethasone Harsen 0.13 mg/kg, and the treatments were given dexamethasone Harsen 0.13 mg/kg with a varieties of vitamins E (Natur-E) multilevel namely P2 (100 mg / kg), P3 (150 mg / kg), and P4 (200 mg / kg). After the treatment was given for 2 weeks, the mice at necropsied and the small intestine were taken for histopathological examination using hematoxyline-eosin staining. Results showed treatment P1 found severe necrosis (caseosa) in the small intestine, while the entire treatment P2, P3, and P4 affect the repair of damage due to effects of the dexamethasone. Treatment 4 (P4) was the best results in reducing the effects of dexamethasone.

Struktur Histopatologi Paru-Paru Tikus Putih yang di Berikan Ekstrak Sarang Semut dan Diinduksi Parasetamol dengan Dosis Toksik I Made Merdana; I Gusti Putu Tovan Mahottama; I Ketut Berata; Luh Made Sudimartini
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Myrmecodia pendan is an epiphytic plant that attaches to large trees. Myrmecodia pendans plant contains chemical compounds such as flavonoids and tannins that are known can cure various diseases. The study aimed to determine the effect of giving myrmecodia pendans extract to the histopathological lung in white rats due to the administration of toxic doses of paracetamol. This study used 24 male white rats and divided into 4 treatment groups. The histopatological changes observed were bleeding, inflammation, congestion and necrosis. The results obtained were the administration of toxic doses of paracetamol orally causes bleeding, inflammation, congestion and necrosis in the lungs of white rats. Giving extract of myrmecodia pendans can improve lung tissue due to the administration of toxic doses of paracetamol which experience bleeding, inflammation, congestion and necrosis in the white rats.

Studi Histopatologi Limpa Anjing Penderita Distemper Dikaitkan Dengan Sebaran Sel-Sel Radang Pada Otak Dan Paru (HISTOPHATOLOGICAL STUDY OF SPLEEN ON DOGS INFECTED WITH DISTEMPER ASSOCIATED TO INFLAMATION IN THE BRAIN AND LUNGS) Muhamad Furkam Fadilah; I Ketut Berata; I Made Kardena
Buletin Veteriner Udayana Vol. 7 No. 2 Agustus 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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This study aim was to determine the distribution of inflammatory cells in canine distemper in terms of the level of inflammation in the spleen, brain and lungs. The sample used 20 infected dogs wihich from the spleens, brains, and lungs of the dogs were collected. These organs were processed for histophatological observation using harris hematoxilyn-eosyn stain. The inflammation of the organs examined by using binocular microscope with 200X magnification. the results showed that inflammation was observed in the spleens: 9 samples (45%) showed the presence of lymphoidcells that experienced a mild inflammation 7 (35%) moderate inflammation, and 4 (20%) severe inflammation in brain, 3 samples (15%) did not show observe inflammation, 12 (60%) mild, 5(25%) moderate inflammation, and not observed any severe inflammation in brain, pulmonary: 6(30%) mild inflammation, 11 (55%) moderate inflammation, and 3 (15%) severe inflammation. It can be concluded that the inflammation was observed microscopically in the spleen, brain and lungin the dog that infected with canine distemper virus

Gambaran Histopatologi Hati Tikus Putih yang Diberikan Ragi Tape Ainul - Hidayah; Putu Suastika; Ketut Budiasa; Samsuri Samsuri; I Ketut Berata; Luh Made Sudimartini
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aimed to determine the histopathological picture of the liver of white rats due to the treatment of yeast at several different doses. A total of 24 white rats were randomly divided into 4 treatment groups, namely P0: control; P1: giving yeast of 100 mg/kg BW; P2: 200 mg/kg BW; and P3: 300 mg/kg BW. The treatment dose was carried out for 21 days. On the 22nd day, all samples got euthanasia and a necropsy procedure was performed. The liver organs were taken to make histological preparations by staining with Hematoxylin Eosin. The variables observed included fatty degeneration and necrosis. The data obtained were analyzed using the Kruskal-Wallis test. The results showed that the administration of yeast at a dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW was not significantly different (P>0.05) compared to the control (P0). It can be concluded that threating by yeast up to a dose of 300 mg/kg BW does not cause histopathological changes in the liver of white rats.

Kajian Retrospektif Gambaran Histopatologi Kasus Streptokokosis Pada Babi Dan Monyet Di Provinsi Bali Laila Gianita Veralyn; I Ketut Berata; I Ketut Eli Supartika
Buletin Veteriner Udayana Vol. 6 No.1 Pebruari 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Penelitian ini bertujuan untuk mengetahui perbandingan distribusi lesi dan tingkat keparahan gambaran histopatologi antara babi dan monyet yang positif menderita streptokokosis dari tahun 1994-1998 di Provinsi Bali. Penelitian ini menggunakan 15 preparat histologi (metode pewarnaan Harris- Hematoksilin-Eosin) dari 15 babi positif streptokokosis dan 7 preparat histologi dari 7 monyet positif streptokokosis. Preparat histologi tersebut berasal dari organ otak, jantung, paru-paru, ginjal dan limpa. Preparat diamati dengan menggunakan mikroskop perbesaran 200x dan 400x. Hasil pemeriksaan histopatologi menunjukkan bahwa distribusi lesi jaringan pada beberapa organ babi dan monyet tidak menunjukkan perbedaan yang signifikan serta tidak terdapat perbedaan tingkat keparahan gambaran histopatologi pada organ otak, jantung, paru-paru dan limpa antara babi dan monyet. Tingkat keparahan gambaran histopatologi yang dominan pada organ otak, paru-paru dan limpa babi maupun monyet yaitu kategori lesi sedang; dicirikan dengan terjadinya kongesti diikuti infiltrasi sel radang neutrofil. Pada organ jantung, baik pada babi maupun monyet sama-sama masuk kategori lesi ringan; dicirikan dengan terjadinya kongesti dan edema ringan. Perbedaan hanya terjadi pada organ ginjal, pada babi dicirikan dengan terjadinya hemoragi sedangkan pada ginjal monyet dicirikan dengan terjadinya kongesti yang diikuti infiltrasi sel radang neutrofil.

STUDI PATOGENESIS PENYAKIT JEMBRANA SAPI BALI BERDASARKAN KARAKTERISTIK SELTERINFEKSI PADA JARINGAN LIMFOID DAN DARAH TEPI I Ketut Berata
Buletin Veteriner Udayana Vol 2 No. 1 Pebruari 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The aim the research is to studi pathogenesis of Jembrana disease on Bali cattlebased on the characteristic of infected cells in lymphoid tissues and peripheral bloodmononuclear cells. The healthy Bali cattle was inoculated with Jembrana disease virus(JDV) (BBVet collection). After the second day of fever, the peripheral blood of experimental cattle was took jugularis vein. Lymphocyte cells from the peripheral bloodwas isolated by picoll-paque gradient method. Then the experimental cattle was necropsied.The spleen, praescapularis lymphnode and praefemoralis lymphnode were took byaseptically, thenits were processed for to histopathological preparation. For to examine thepercentage of the infected cells in lymphoid organs, the part of each lymphoid tissues weremade suspension in phosphat buffer saline, then it was made smear preparation on theobject glass. Those peripheral lymphocyte cells, lymphocyte smears and histopathologicalpreparation were stained by indirect immunoperoxidase technique. The JDV infected cellswas appeared brown color. The intensity of brown color was examined for to determinedegree of the infection.The result showed that percentage of JDV infected cells in bothspleen andlymphnodes are similarly i.e. average 9,5%. Percentage of the JDV infected cellson peripherallymphocyte cell was average 7%. Based on the intensity of brown color wasappeared that the JDV infected cells from the lymphnodes were stronger than from spleen.The conclusion is the most of the port d‘entry JDV is through the subcutaneus route.

Aktivitas Angiogenesis Gel Extract Biji Cacao pada Penyembuhan Luka Insisi Gusi Marmut Ketut Novi Kusmayani; Anak Agung Gde Jaya Warditha; I Ketut Berata
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

One of alternative herbal therapy ingredients, cocoa beans because it contains flavonoids, polyphenols, and alkaloids can reduce number of macrophages and antioxidants, to accelerate inflammatory process. This study was aimed determine effect cocoa bean extract gel on healing guinea pig gum incisions based on level angiogenesis. This study was experimental study using completely randomized design, with four treatments with 6 replications, total of 24 experimental animals. Guinea pigs were injected with ketamine at dose of 50 mg/kg BW anesthetic, then incision made in gums with length 10 mm and depth 2 mm. Then given treatment of topical cocoa bean extract gel, (T0) group with concentration 0%, (T1) 16%, (T2) 24%, (T3) 32%, each for 5 days. On 6th day euthanasia was performed and gum tissue was taken. Gum tissue was processed preparation of histology preparations using Hematoxylin-Eosin (HE) staining method. Histopathological examination preparations were carried out with a microscope based on level angiogenesis. Results of histopathological examination obtained an average level angiogenesis at To=19.00, T1=38.00, T2=43.50, T3=47.83. Statistical analysis showed that there was a significant increase in angiogenesis between controls (T0) compared to group given cocoa bean extract gel (T1, T2 and T3). But between treatment groups T1, T2 and T3 there was no significant difference. It can conclude topical application of cocoa bean extract gel has been shown increase angiogenesis in guinea pig gum incision wound healing. Administration of cocoa bean extract with a concentration of 32% resulted in highest angiogenesis rate.

Co-Authors Afrizal Choirul Umam Agnes Endang Tri H Aida Lousie Tenden Rompis Ainul - Hidayah Alviana Rizqiyah Utami Amelia, Ni Kadek Shita Anak Agung Ayu Mirah Adi Anak Agung Gde Arjana Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Anak Agung Gde Jaya Warditha Anak Agung Oka Anak Agung Sagung Kendran Apsari, Ni Luh Putu Nadia Ardi Sandriya Aristawati, I Dewa Agung Ayu Irma Astari, Ni Putu Widya Astini, Ni Putu Sri Ayu Aulia Insani, Aulia Ayu Prawitasari Citra Pratama Bina Ichsantya Budiartawan, I Komang Alit Damara, Doni Darmawan, I Gusti Ayu Chintya Darmayanti, Mahda Dwi Devira, Dinda Dewa Ayu Dwita Karmi Dhinar Wahyu Prasetyo Dini Hilary Manullang DORTY PRIHASTINA SALBAHAGA Duwiri, Christine Valeri DWI SURYANTO Dzikri Nurma'rifah Takariyanti Elisabeth Karina Elsa Hidayati Elti Febilani Elyda . Erwanti Siti Rabiah, Erwanti Siti Farhan Abdul Hasan, Farhan Abdul Febrianty, Ni Made Dhea Fitri Irawan Rahmawandani Franky Samuel Milenyano Chandra Gde Jasmara Muda Ginting, Regina Bonifasia Br Gunawan, Stefanus Andre Gunawati, Luh Sri Guru, Yohana Cendyka Kartika Dewi Gusti Agung Ayu Putu Adriyati, Gusti Agung Ayu Hendrina Konda M Meha, Hendrina Konda M Humaira, Sarah Husnul Khatimah I Dewa Made Adhiwitana I GEDE ENDRA KUSUMA I Gusti Bagus Sathya Dharma, I Gusti Bagus Sathya I Gusti Ketut Suarjana I Gusti Ngurah Kade Mahardika I Gusti Putu Tovan Mahottama I Ketut Eli Supartika I Ketut Eli Supartika I KETUT ELI SUPARTIKA I Ketut Puja I Ketut Sumadi I Ketut Wirata I Made Damriyasa I Made Indrayadnya Swarayana I Made Kardena I Made Merdana I Made Putra Wiadnyana I Made Sukada I N. T. ARIANA I NYOMAN ADI SURATMA I NYOMAN MANTIK ASTAWA I Nyoman Sulabda I Putu Suparman I W BUDIARSA SUYASA I Wayan Sudira I Wayan Wirata Ida Ayu Pasti Apsari Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Iwan Harjono Utama Janardani, Ni Made Kunti Jayawardhita, Anak Agung Gde Kadek Karang Agustina Kadek Karina Dewi Wijayanthi Kartika, Erena Hajar Ketut Budiasa Ketut Eli Supartika Ketut Novi Kusmayani Ketut Tono Pasek Gelgel Kevin Dominika Kristi Agusti Putri, Kristi Agusti Kusuma, Putu Winatha Laila Gianita Veralyn Luh Made Sudimartini Lusiana Flora Ndagu Made Oka Adinata Made Rahayu Kusumadewi Marissa Divia Dayanti Marson, Fransiska Gratia Sonita MAS DJOKO RUDYANTO Muhamad Furkam Fadilah Muhsi, Ach Moh Abd Nesia Masniari Helena Sibarani Ni Luh Eka Setiasih Ni Nyoman Werdi Susari Nofantri, Lidia Noviriolla Maria Nugraha, Putri Oktaviandari, Putu Risma Palagan Senopati Sewoyo Pangesti, Thiara A. Pramesti, Ni Komang Lady Prista Oktafebri Yulestari Pujaswarini, Ni Made Hani Purnama, Komang Andika Purnata, I Dewa Nyoman Alit Putra, I Putu Agus Antara Putri Yuliana Mangindaan Putu Agus Trisna Kusuma Antara Putu Suastika Raharjo, Yudha Yaksa Crada Yoga Arum Rahmat Grahadi RAHMI MUSTABA Sam suri Samsuri Samsuri Sari Sartini, Sari Setianingsih, Ni Luh Eka Setyawati, Luh Gede Sewoyo, Palagan Senopati Sihotang, Tanti Fitri Sri Kayati Widyastuti Suranjaya I .Gd Tanuwijaya, Phebe Amadea Vaswani Samaria Napitupulu Wahyu Semadi Putra Waskitha, Melati Pusparini Widia Insani Wulandari, Meidi Andira Yanne Yanse Rumlaklak Yesi Veronica Sitepu Yustisia, Anggia Zumara Mufida Hidayati

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