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All Journal Journal of Agromedicine and Medical Sciences Sosio Konsepsia Dentino: Jurnal Kedokteran Gigi Berkala Kedokteran Sari Pediatri Jurnal Sinergitas PkM & CSR MAGNA MEDICA: Berkala Ilmiah Kedokteran dan Kesehatan Bioinformatics and Biomedical Research Journal Prosiding Konferensi Nasional PKM-CSR Homeostasis: Jurnal Mahasiswa Pendidikan Dokter DUNIA KEPERAWATAN: JURNAL KEPERAWATAN DAN KESEHATAN
Lia Yulia Budiarti
Microbiology Department, Faculty Of Medicine Lambung Mangkurat University Banjarmasin
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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MINIMUM INHIBITORY CONCENTRATION OF WHITE GINGER AND CHLORHEXIDINE GLUCONATE ON ACRYLIC PLATES TOWARD Candida albicans Saputera, Debby; Aulia Nalar, Gusti; Budiarti, Lia Yulia
Dentino Vol 2, No 1 (2017)
Publisher : FKG Unlam

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ABSTRACTBackground: Acrylic resin-based dentures are commonly used nowadays. The hygiene of denture base can be maintained by soaking the denture base into 0.2% chlorhexidine gluconate. Unhygienic denture base can lead to denture stomatitis. Flora accumulation, such as Candida albicans, may occur. White ginger is active towards Candida albicans, with antifungal properties due to its phenol compound. Aim: This study aims to determine the Minimum Inhibitory Concentration value (MIC) of the ethanol extract of white ginger towards Candida albicans growth in heat cured removable acrylic dentures. Methods: This is an experimental study with a post test only control group design. Acrylic resin were soaked in treatment extract of 30%, 35%, 40%, 45%, 50%, 60%, 70%, 80%, 90%, 100% concentration,0.2% chlorhexidine gluconate and 70% ethanol as the control. One Way ANOVA test and Bonferroni Post Hoc test with 95% confidence level were used. Results: The result of the study shows that the MIC value of 40%, 45%, 50%, 60%, 70%, 80% 90% and 100% of treatment extract were respectively 13:07%; 18:36%; 23.67%; 28.87%; 36.84%; 42.10%; 49.98%; 52.61%. Conclusion: This study concluded that the treatment extract can reduce the amount of Candida albicans,100% concentration made the strongets antifungal effect compared to lesser concentrations and 0.2% chlorhexidine gluconate.Key words: Candida albicans, chlorhexidine gluconate, denture stomatitis, heat cured acrylic resin, MIC, white ginger.
UJI EFEKTIVITAS ANTIBAKTERI AIR PERASAN JERUK NIPIS (CITRUS AURANTIFOLIA) SEBAGAI BAHAN IRIGASI SALURAN AKAR ALAMI TERHADAP PERTUMBUHAN ENTEROCOCCUS FAECALIS IN VITRO Ramadhinta, Talitha Maghfira; Ichrom Nahzi, Muhammad Yanuar; Budiarti, Lia Yulia
Dentino Vol 1, No 2 (2016)
Publisher : FKG Unlam

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ABSTRACT Background: Lime (Citrus aurantifolia) is one of the plants which have antimicrobial activity. Acid substance in lime juice is coagulant source. Citric acid in lime juice has the quality to prevent bacterial and fungal growth. Enterococcus faecalis is known as the most resistant species in oral cavity and commonly found in post-root canal treatment cases. E.faecalis was reported in 20 of 30 persistent infected endodontic teeth after root canal treatment. The aim of this study to assess lime (Citrus aurantifolia) juice’s in vitro inhibition activity against Enterococcus faecalis. Purpose: This study used laboratory experimental method with postest only control group design using total random sampling; consisted of 6 treatments and 5 times repetition. Methods: Antibacterial activity test was performed using diffusion method. Data was analyzed using one way anova with confidence interval of 95% and the result presented that there’s a significant difference between β5%, 50%, 75%, 100% lime juice treatment groups and 3% hydrogen peroxide treatment group. Result:Based on the result of post hoc LSD, the conclusion of this study was 100% lime juice has better inhibition activity than 25%, 50%, 75% lime juice and 3% hydrogen peroxide. Conclusion: There was a difference of antibacterial effectiveness between lime juice and 3% H2O2 on Enterococcus faecalis bacterial growth observed from their inhibition zones.  ABSTRAK Latar Belakang: Jeruk Nipis (Citrus aurantifolia) merupakan salah satu tanaman yang mempunyai aktivitas antimikroba. Kandungan asam pada air perasan jeruk nipis merupakan sumber koagulan. Senyawa asam sitrat dalam air perasan jeruk nipis mampu mencegah pertumbuhan bakteri dan jamur. Enterococcus faecalis dikenal sebagai spesies yang paling resisten pada rongga mulut dan paling sering ditemukan pada kasus setelah perawatan saluran akar. E.faecalis ditemukan sebanyak 20 dari 30 kasus infeksi endodontik yang persisten pada gigi yang telah dilakukan perawatan saluran akar. Tujuan: Tujuan Penelitian ini adalah untuk mengetahui daya hambat air perasan buah jeruk nipis (Citrus aurantifolia) terhadap pertumbuhan bakteri Enterococcus faecalis secara in vitro. Metode: Penelitian ini dilakukan dengan metoda eksperimental laboratorium; postest only control group design dengan rancangan acak lengkap menggunakan 6 perlakuan dan 5 kali pengulangan. Metode uji aktivitas antibakteri menggunakan metode difusi. Analisis data menggunakan uji one way anova 95% menunjukkan terdapat perbedaan bermakna antara perlakuan air perasan jeruk nipis konsentrasi 25%, 50%, 75%, 100% dan perlakuan hidrogen peroksida 3%. Hasil: Pada hasil uji post hoc LSD diambil kesimpulan perlakuan air perasan jeruk nipis konsentrasi 100% memiliki efek daya hambat lebih baik dibandingkan  konsentrasi di bawahnya dan dibandingkan dengan perlakuan hidrogen peroksida 3%. Kesimpulan: Terdapat perbedaan efektivitas antibakteri dari air perasan jeruk nipis dan H2O2 3% terhadap pertumbuhan bakteri Enterococcus faecalis dilihat dari zona hambat.
THE COMPARISON OF ANTIFUNGAL EFFECT BETWEEN GARLIC EXTRACTS AND ALKALINE PEROXIDE TOWARDS CANDIDA Zohra, Noor Fathimah; Saputera, Debby; Budiarti, Lia Yulia
Dentino Vol 2, No 2 (2017)
Publisher : FKG Unlam

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Background: Candida albicans is a microorganism found on denture plaque. Candida albicans canpenetration and accumulated on surface of the denture and then to infection the soft tissue that causes denturestomatitis. Denture cleanser has effectivity of anti-fungal is alkaline peroxide. Naturally denture cleansercontain of anti-fungal is garlic. Purpose: This research is to analyze inhibition effectivity of garlic extractconcentration 2,5%, 5%, 10%, 12,5%, 15%, and alkaline peroxide to Candida albicans growth on acrylic heatcured. Method: This research uses true experimental with post test only with control group design. Garlicextract concentration 2.5%, 5%, 10%, 12.5%, 15%, and alkaline peroxide. The method of this research usesdiffusion method with the measure of inhibition zone. Result: The result of the research garlic extractconcentration 2.5%, 5%, 10%, 12.5%, 15%, and alkaline peroxide to Candida albicans growth have inhibitionzone are 11.29mm, 13.25mm, 16.30mm, 18.2mm, 19.46mm, and 15.33mm. The result of normality test withSaphiro-Wilk is p>0,05, Levene’s test that results is p=0,575 (p>0,05) so the all data are normality andhomogenity. Analyze data uses One Way ANOVA that result is p=0,000 (p<0,05) are differences inhibitioneffectivity and next testing of Post Hoc Bonferroni show that significant differences between each group.Conclusion: The research is the differences inhibit effectivity of the garlic extract concentration 2,5%, 5%,10%, 12,5%, 15%, and alkaline peroxide to Candida albicans growth on acrylic heat cured. Garlic extract in10%, 12.5% and 15% have a bigger effectivity of inhibition than alkaline peroxide to Candida albicans growth.
EFEKTIVITAS ANTIFUNGI EKSTRAK METANOL BATANG PISANG MAULI (Musa acuminata) DAN Chlorhexidine gluconate 0,2% TERHADAP Candida albicans Permatasari, Dita; Budiarti, Lia Yulia; Laillyza Apriasari, Maharani
Dentino Vol 1, No 1 (2016)
Publisher : FKG Unlam

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ABSTRACT Background: Mauli banana stem extract has antifungal effectiveness against Candida albicans because it has flavonoid, saponin, and tannin substances. Chlorhexidine gluconate 0,2% is widely used to treat oral candidiasis caused by Candida albicans fungal infection. Chlorhexidine gluconate 0,2% is known to have caused side effects. Purpose: The purpose of this study is to knowing the antifungal effectiveness differences between 25%, 80%, 100% mauli stem methanol extracts and chlorhexidine gluconate 0,2% against Candida albicans. Methods: This study was true experimental with post test-only with control group design consisted of 4 treatment groups. Treatment group I,II,III each was given 25%, 80%, 100% mauli banana stem methanol extract respectively and treatment group IV was given chlorhexidine gluconate 0,2% as positive control group. The repetition of each treatment was 7 times. Antifungal effectiveness was assessed by measuring inhibition zone of Candida albicans growth using diffusion method. Results: This research showed that mean inhibition zone of treatment group I (13 mm), treatment group II (17 mm), treatment group III (19 mm), and treatment group IV (21 mm). One-way Anova test indicated that there was significant difference between 25%, 80%, 100% mauli banana stem methanol extracts and chlorhexidine gluconate 0,2%. Conclusion: Based on the Post-hoc Bonferroni test, it can be concluded that 100% concentration mauli banana stem methanol extract had more prominent antifungal effectiveness than 25% and 80% against Candida albicans but still less effective than chlorhexidine gluconate 0,2%.Keywords: mauli banana stem extract, antifungal effectiveness, Candida albicans, chlorhexidine gluconate 0,2%ABSTRAK Latar Belakang: Ekstrak batang pisang mauli memiliki efektivitas antifungi terhadap Candida albicans karena mengandung senyawa flavonoid, saponin, dan tanin. Chlorhexidine gluconate 0,2% biasanya digunakan untuk mengobati kandidiasis oral yang disebabkan oleh infeksi jamur Candida albicans. Chlorhexidine gluconate 0,2% diketahui dapat menimbulkan efek samping. Tujuan: Tujuan penelitian ini adalah untuk mengetahui perbedaan efektivitas antifungi ekstrak metanol batang pisang mauli konsentrasi 25%, 80%, 100% dan chlorhexidine gluconate 0,2% terhadap Candida albicans. Metode: Penelitian ini merupakan penelitian eksperimental murni dengan post test-only with control group design yang terdiri dari 4 perlakuan. Perlakuan I,II,III masing-masing diberikan ekstrak metanol batang pisang mauli konsentrasi 25%, 80%, 100% dan perlakuan IV diberikan chlorhexidine gluconate 0,2% sebagai kontrol positif. Jumlah pengulangan setiap perlakuan adalah 7 kali. Efektivitas antifungi dinilai dari mengukur zona hambat pertumbuhan Candida albicans dengan metode difusi. Hasil: Penelitian ini menunjukkan bahwa rata-rata zona hambat yang terbentuk pada perlakuan I (13 mm), perlakuan II (17 mm), perlakuan III (19 mm), dan perlakuan IV (21 mm). Uji Oneway Anova menunjukkan bahwa terdapat perbedaan bermakna antara efektivitas antifungi ekstrak methanol batang pisang mauli konsentrasi 25%, 80%, 100% dan chlorhexidine gluconate 0,2%.Kesimpulan: Berdasarkan uji Post-hoc Bonferroni dapat disimpulkan bahwa efektivitas antifungi ekstrak metanol batang pisang mauli konsentrasi 100% lebih besar dibandingkan 25% dan 80%, tetapi masih kurang efektif dengan chlorhexidine gluconate 0,2%.
UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN TUNGGAL DIBANDINGKAN KOMBINASI SEDUHAN DAUN TEH HIJAU (Camellia sinensis) DAN MADU (Studi in Vitro terhadap Jumlah Koloni Bakteri Rongga Mulut) Tinjauan pada Mahasiswa PSKG FK Unlam Banjarmasin Angkatan 2011-2013 A, Wahyuni; Dewi, Nurdiana; Budiarti, Lia Yulia
Dentino Vol 1, No 2 (2016)
Publisher : FKG Unlam

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ABSTRACTGreen tea has various active substances; one of them is polyphenol, mostly cathechin, an active compound which can protect teeth from caries because of its anti-streptococcal activity. A high content of minerals in honey has alkali characteristic thus the disinfectant trait in oral cavity. The aim of this study was to assess whether single and combined preparations of brewed green tea and honey can decrease bacterial colony count in oral cavity. This study was quasi experimental with pretest and posttest controlled group design. Samples of 38 Students of Dentistry Study Program Universitas lambung Mangkurat were divided into 19 groups: 3 groups were given 25%, 50%, 100% green tea in single preparations, 4 groups were given 6,25%, 12,5%, 25%, 50% honey in single preparations, and 12 groups were given combined preparations of green tea and honey. Bacterial colony before and after gargling grown on isolated medium was counted using colony counter. Repeated anova test showed p value of 0,037 (p < 0,05), stating that there was a significant difference among treatment groups. In conclusion, combined preparations of 50% green tea + 12,5% honey and 100% green tea + 25% honey were more effective in decreasing bacterial colony count.   ABSTRAKTeh hijau mempunyai beberapa komponen aktif yang salah satunya adalah polifenol berupa katekin, suatu senyawa aktif dalam melindungi gigi dari karies karena memiliki aktivitas anti-streptococcal. Kandungan mineral yang tinggi pada madu mempunyai sifat basa (mengandung unsur alkali) sehingga dapat berfungsi sebagai desinfektan terhadap rongga mulut. Penelitian ini bertujuan untuk mengetahui apakah sediaan tunggal dan kombinasi seduhan daun teh hijau dan madu dapat menurunkan jumlah koloni bakteri rongga mulut. Penelitian ini bersifat eksperimental kuasi dengan rancangan pretest and posttest controlled group design. Sampel sebanyak 38 orang mahasiswa Program Studi Kedokteran Gigi Fakultas Kedokteran yang dibagi dalam 19 kelompok yaitu sebanyak 3 kelompok perlakuan sediaan tunggal teh hijau menggunakan konsentrasi 25%, 50%, dan 100%, sebanyak 4 kelompok perlakuan sediaan tunggal madu menggunakan konsentrasi 6,25%, 12,5%, 25%, dan 50%, sebanyak 12 dengan kelompok perlakuan sediaan kombinasi teh hijau dan madu. Jumlah koloni yang tumbuh pada media isolasi sebelum dan sesudah berkumur dihitung menggunakan alat colony counter. Hasil uji Repeated anova menunjukan nilai p = 0,037 (p < 0,05), terdapat perbedaan yang bermakna diantara perlakuan. Dapat disimpulkan bahwasediaan kombinasi teh hijau 50% dengan madu 12,5% dan teh hijau 100% dan madu 25% lebih efektif dalam menurunkan jumlah koloni bakteri.
AKTIVITAS DAYA HAMBAT EKSTRAK ETANOL DAUN BELIMBING WULUH DAN SODIUM HIPOKLORIT TERHADAP Enterococcus faecalis (In Vitro) Nisa, Rohmatun; Erlita, Isyana; Budiarti, Lia Yulia
Dentino Vol 2, No 2 (2017)
Publisher : FKG Unlam

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Background: Enterococcus faecalis is gram-positive bacteria that cause the failure of root canal treatment. Effective method to eliminate Enterococcus faecalis in root canal by using sodium hypochlorite (NaOCl) 5.25%. Natural materials can be inhibiting the growth of gram-positive bacteria including Enterococcus sp. is a compound of tannins, saponins and flavonoids. Purpose: This study aims to know the differences of inhibition of the activity in ethanol extract of wuluh starfruit leaves (Averrhoa bilimbi L.) and sodium hypochlorite (NaOCl) 5.25% against Enterococcus faecalis. Methods: This study was using true experimental research design, post test-only with control group design that the treatments which being tested were ethanol extract of starfruit leaf 40%, 50%, 60% and NaOCl 5.25%. Antibacterial effect tested with diffusion method and the parameters measured by the amount of inhibition zone formed in the media of the test. The data analysis using Kruskall-Wallis test and next testing of Mann Whitney. Results: The results showed that ethanol extract of wuluh starfruit leaf with concentration 40%, 50%, 60% and NaOCl 5.25% against Enterococcus faecalis as measured from inhibition zone are 13.37 mm, 16.50 mm, 18 39 mm and 21.30 mm. The data analysis using Kruskall-Wallis test, the results this research is p=0.000 (p<0.05) then there are a differences in the inhibitory activity between each group, and next testing of Mann Whitney that results is p=0.002 (p<0.05) show meaningful differences between each group. Conclusion: The conclusion is the differences in the inhibitory activity of ethanol extract of starfruit leaf extract and sodium hypoclorite 5,25% against Enterococcus faecalis.
EFEK ANTIBAKTERI SEDIAAN TUNGGAL DAN KOMBINASI AIR PERASAN JERUK NIPIS DAN MADU TERHADAP Streptococcus mutans Kajian In Vitro SediaanTunggal dan Kombinasi Air Perasan Jeruk Nipis dan Madu dengan Klorheksidin Glukonat 0,2% Fitriani, Alfia; Dewi, Nurdiana; Budiarti, Lia Yulia
Dentino Vol 1, No 2 (2016)
Publisher : FKG Unlam

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ABSTRACT  Background: Streptococcus mutans is a species of gram positive bacteria dominantly residing in oral cavity and also acts as the most common pathogen causing caries. Lime juice and honey are herbal medicine which has been proven to possess antibacterial effect. Purpose: The aim of this study was to verify that lime juice and honey in combined preparation had more favorable inhibitor potency compared to single preparation against Streptococcus mutans. Methods: This were an experimental study which using random sampling, with 26 treatment groups : 25%, 50%, 75%, 100% lime juice in single preparations and 6,25%, 12,5%, 25%, 50% honey in single preparations, combined preparations of lime juice and honey, also 0,2 chlorhexidin gluconate and aquadest as negative control group. Antibacterial activity test was performed using diffusion method.Result: Data observed showed that there were combined preparations with better inhibitor potency compared to single preparations. Conclusion:One Way ANOVA and LSD tests with confidence interval of 95% presented that antibacterial activity of lime juice and honey in combined preparation was better than its single counterpart against Streptococcus mutans.   Keywords :  antibacterial, lime juice, honey, Streptococcus mutans, inhibition zone  ABSTRAK  Latar Belakang: Streptococcus mutans merupakan salah satu spesies bakteri gram positif yang dominan dalam mulut dan merupakan bakteri penyebab karies patogen paling banyak menyerang manusia. Air perasan jeruk nipis dan madu merupakan tanaman obat yang terbukti mempunyai efek antibakteri.Tujuan: Penelitian ini bertujuan untuk membuktikan bahwa sediaan kombinasi air perasan jeruk nipis dan madu mempunyai daya hambat lebih besar daripada sediaan tunggal dalam menghambat Streptococcus mutans. Metode:Penelitian ini merupakan penelitian eksperimental dengan rancangan acak kelompok yang terdiri dari 26 perlakuan, yaitu sediaan air perasan jeruk nipis konsentrasi 25%, 50%, 75%, 100%, dan madu konsentrasi 6,25%, 12,5%, 25%, 50%, sediaan kombinasinya, serta klorheksidin glukonat 0,2% dan akuades sebagai kontrol negatif. Metode uji aktivitas antibakteri yang digunakan pada penelitian ini adalah metode difusi.Hasil: Data yang didapat menunjukkan bahwa terdapat beberapa perlakuan sediaan kombinasi lebih baik dari sediaan tunggal. Kesimpulan:Hasil uji One Way ANOVA dan LSD dengan tingkat kepercayaan 95% menunjukkan bahwa aktivitas antibakteri sediaan kombinasi air perasan jeruk nipis dan madu lebih baik dari sediaan tunggalnya dalam menghambat pertumbuhan Streptococcus mutans.  Kata-kata kunci : antibakteri, air perasan jeruk nipis, madu, Streptococcus mutans, zona hambat
PERBANDINGAN EFEKTIVITAS ANTIFUNGI ANTARA EKSTRAK METANOL KULIT BATANG KASTURI DENGAN KETOKONAZOL 2% TERHADAP Candida albicans IN VITRO Siddik, Muhammad Baihaqi; Budiarti, Lia Yulia; Edyson, Edyson
Berkala Kedokteran Vol 12, No 2 (2016)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v12i2.1877

Abstract

Abstract: Candida albicans is the most common cause of candidacies. Ketoconazole is one of the main options treatment of candidiasis, but is reported to have experienced resistance and hepatotoxic. Extract methanol bark of kasturi contains the same active substance with, Mango that is phenolic groups, terpenoids, and saponins that are antifungal. The purpose of this study was to determine differences in the concentration of the methanol extract of the bark of kasturi with ketoconazole 2% against Candida albicans in vitro. This study was true laboratory experimental  by using randomize post test-only group designs, which consisted of 9 treatments, ie EMKBK concentration of 25%, 50% to 37.5%, 62.5%, 75%, 87.5%, 100%, ketoconazole 2% and 70% methanol (control) repetition 3 times with diffusion test. Data analysis using ANOVA and post hoc LSD test (α = 0.05). The result showed mean inhibition zone the methanol extract of the bark of kasturi against Candida albicans at a concentration of 25%, 37.5%, 50%, 62.5%, 75%, 87.5% and 100% is 7 mm; 9 mm; 10 mm; 12 mm; 16 mm; 19 mm, 22 mm and ketoconazole 2% is 15 mm and there is a significant difference between the treatment EMKBK with ketoconazole 2%. Keywords: antifungal, extract methanol bark of kasturi, ketokonazole 2%, Candida albicans  Abstrak: Candida albicans merupakan penyebab tersering kandidiasis. Ketokonazol merupakan salah satu pilihan utama untuk mengobati kandidiasis, tetapi dilaporkan telah mengalami resistensi dan bersifat hepatotoksik. Ekstrak metanol kulit batang kasturi mengandung zat aktif yang sama dengan mangga yaitu golongan fenolik, terpenoid, dan saponin yang merupakan antifungi. Tujuan penelitian ini untuk mengetahui perbedaan konsentrasi ekstrak metanol kulit batang kasturi dengan ketokonazol 2% terhadap Candida albicans In Vitro. Penelitian ini adalah eksperimental laboratoris murni dengan mengunakan randomize post test- only group designs , yang terdiri dari 9 perlakuan, yaitu EMKBK konsentrasi 25 %, 37,5 % 50 %, 62,5 %, 75 %, 87,5 %, 100 %, ketokonazol 2% dan metanol 70% (kontrol) pengulangan 3 kali dengan uji difusi. Analisis data mengunakan uji ANOVA dan uji post hoc LSD (α=0,05). Hasil penelitian didapatkan rerata zona hambat ekstrak metanol kulit batang kasturi terhadap Candida albicans pada konsentrasi 25%, 37,5%, 50%, 62,5%, 75%, 87,5% dan 100% adalah 7 mm; 9 mm; 10 mm; 12 mm;16 mm; 19 mm, 22 mm dan ketokonazol 2% adalah 15 mm dan terdapat perbedaan bermakna antara perlakuan EMKBK dengan ketokonazol 2%. Kata – kata kunci: antifungi, ekstrak metanol kulit batang kasturi, ketokonazol 2%, Candida albicans
Perbandingan Efektivitas Antibakteri antara Ekstrak Metanol Kulit Batang Kasturi dengan Ampisilin terhadap Staphylococcus aureusin Vitro Akbar, M. Rizki Valian; Budiarti, Lia Yulia; Edyson, Edyson
Berkala Kedokteran Vol 12, No 1 (2016): Februari 2016
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v12i1.350

Abstract

Abstract:Kasturi as a typical plant in South Kalimantan is one fruit that has many benefits. The barks of kasturi has proved to have benefits, especially to inhibit the activity of Staphylococcus aureus. Staphylococcus aureus is a gram positive bacteria that cause pneumonia, mastitis, and urinary tract infections. The bark of kasturi can be used in extract form. The objective of this research is to know the difference between the preparations of inhibition kasturi’s bark extract and ampicillin 30μg in inhibiting the growth of Staphylococcus aureus in vitro. This study used an experimental method consisting of 9 treatments with 3 repetitions. Treatment test in the methanol extract of the bark of kasturi 25%, 37.5%, 50%, 62.5%, 75%, 87.5% and 100%. The control group used ampicillin and 70% methanol. Bacterial test was done by using a diffusion method. The parameter measured was the amount of inhibition zone (mm) which grown on media MH. Analysis of study data used One way Annova test and Post Hoc LSD test at α=0,05. The results showed that there were significant differences between the treatment kasturi’s bark extract 25%, 50%, 62.5%, 75%, 87.5% and 100% concentration different compared to ampicillin. Meanwhile, at the concentration of 37.5% (p <0.05) was not significant. The antibacterial effectiveness was obtained from the concentration of 100%. Key words: the bark of kasturi, methanol extract, Staphylococcus aureus, inhibition zone. Abstrak: Kasturi sebagai salah satu tanaman khas yang ada di Kalimantan Selatan merupakan salah satu buah yang memiliki banyak khasiat. Kulit batang kasturi terbukti memiliki manfaat terutama dapat menghambat aktivitas Staphylococcus aureus. Staphylococcus aureus merupakan bakteri gram positif yang menyebabkan penyakit pneumonia, mastitis, dan infeksi saluran kemih. Kulit batang kasturi dapat digunakan dalam bentuk ekstrak. Tujuan penelitian ini mengetahui perbedaan daya hambat antara sediaan ekstrak kulit batang kasturi dengan ampisilin 30µg dalam menghambat pertumbuhan bakteri Staphylococcus aureus secara in vitro. Penelitian ini menggunakan metode eksperimental yang terdiri dari 9 perlakuan dengan 3 kali pengulangan. Perlakuan yang di uji adalah ekstrak metanol kulit batang kasturi 25%, 37,5%, 50%, 62,5%, 75%, 87,5% dan 100%. Dan kontrol perlakuan dengan ampisilin dan metanol 70%. Uji bakteri dilakukan menggunakan metode difusi. Parameter yang diukur adalah besaran zona hambat (mm) yang tumbuh pada media MH. Analisis data penelitian mengunakan uji One way ANNOVA dan uji Post Hoc LSD pada α=0,05. Hasil penelitian menunjukan bahwa terdapat perbedaan bermakna diantara perlakuan ekstrak kulit batang kasturi 25%, 50%, 62,5%, 75%, 87,5% dan 100% konsentrasi yang beda di bandingkan dengan ampisilin. Sedangkan pada konsentrasi 37,5% (p<0,05) tidak bermakna. Efektivitas antibakteri yang terbesar di peroleh dari konsentrasi 100%.Kata-kata kunci: kulit batang kasturi, ekstrak metanol, Staphylococcus aureus, zona hambat.
Pola Resistensi Bakteri Kontaminan Luka Pasien di Bangsal Bedah Ortopedi RSUD Ulin Banjarmasin Periode Juli-September 2013: Tinjauan In Vitro Pola Resistensi Isolat Bakteri Kontaminan Asal Swab Luka Pasien di Bangsal Bedah Ortopedi RSUD Ulin Banjarmasin Terhadap Gentamisin, Kloramfenikol, Sefotaksim dan Seftriakson Rihansyah, Akbar; Putera, Husna Dharma; Budiarti, Lia Yulia
Berkala Kedokteran Vol 10, No 2 (2014): September 2014
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v10i12.964

Abstract

ABSTRACT: Surgery, trauma, burns, and other factors can affect the defense/skin barrier against bacterial contamination that can cause infection. The risk of infection must be remained of the rational use of prophylactic antibiotics. Rational use of antibiotic susceptibility test results obtained by antibotic against bacteria. The aim of this research was to figure out the resistance pattern of bacteria contaminant in patient’s wound at Orthopaedic Ward of RSUD Ulin Banjarmasin to selected antibiotics i.e. gentamicin, chloramphenicol, cefotaxime and ceftriaxone from July-September 2013. This was descriptive research. The samples were taken with consecutive sampling technique according to inclusion criteria. This research used wound swab bacteria contaminant isolates i.e. Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Streptococcus sp. Antibiotic susceptibility test was done in vitro with Kirby-Bauer diffusion method. The radical zones were measured and compared to CLSI 2011 standard. The antibiotic susceptibility test result showed that Staphylococcus aureus was sensitive to gentamicin (100%) and cefotaxime (66,67%), resistant to chloramphenicol (44,44%); Staphylococcus epidermidis was sensitive to cefotaxime (28,75%), resistant to gentamicin (85,71%) and chloramphenicol (57,14%); Pseudomonas aeruginosa was sensitive to cefotaxime (33,33%), resistant to ceftriaxone (66,67%); Streptococcus sp. was sensitive to cefotaxime (50%), resistant to gentamicin (50%), chloramphenicol (100%) and ceftriaxone (50%). Key words:   Antibiotic susceptibility, wound bacterial contaminant. ABSTRAK: Tindakan operasi, trauma, luka bakar dan beberapa faktor lain dapat mempengaruhi pertahanan/barier kulit terhadap kontaminasi bakteri yang dapat menyebabkan infeksi. Risiko terjadinya infeksi harus tetap diwaspadai dengan penggunaan antibiotik profilaksis yang rasional. Penggunaan antibiotik rasional didapatkan berdasarkan hasil uji kepekaan antibotik terhadap bakteri penyebab. Penelitian ini bertujuan untuk mengetahui pola resistensi bakteri kontaminan pada luka pasien di Bangsal Bedah Ortopedi RSUD Ulin Banjarmasin terhadap beberapa antibiotik yaitu gentamisin, kloramfenikol, sefotaksim dan seftriakson periode Juli-September 2013. Penelitian ini bersifat deskriptif. Pengambilan sampel menggunakan teknik consecutive sampling menurut kriteria inklusi. Penelitian ini menggunakan isolat bakteri kontaminan hasil swab luka pasien yaitu Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, dan Streptococcus sp. Uji kepekaan keempat jenis bakteri tersebut dilakukan secara in vitro dengan metode difusi Kirby-Bauer. Zona radikal yang terbentuk diukur dan dibandingkan dengan standar CLSI 2011. Hasil uji kepekaan antibiotika menunjukkan bahwa Staphylococcus aureus sensitif terhadap gentamisin (100%) dan sefotaksim (66,67%), resisten terhadap kloramfenikol (44,44%); Staphylococcus epidermidis sensitif terhadap sefotaksim (28,75%), resisten terhadap gentamisin (85,71%) dan kloramfenikol (57,14%); Pseudomonas aeruginosa sensitif terhadap sefotaksim (33,33%), resisten terhadap seftriakson (66,67%); Streptococcus sp. sensitif terhadap sefotaksim (50%), resisten terhadap gentamisin (50%), kloramfenikol (100%) dan seftriakson (50%). Kata-kata kunci : Kepekaan antibiotika, bakteri kontaminan luka.
Co-Authors A.A. Ketut Agung Cahyawan W Abdurahman Wahid Agung Biworo Agung Biworo Aisyah Aisyah Akbar Rihansyah, Akbar Alfi Yasmina Alfi Yasmina Alfia Fitriani, Alfia Alshazil, Renata Seikh Amalia, Maulidia Khairada Amalya, Khalida Zikra Amiratun Naillah Anward, Aliy Arivin Ari Yunanto Arietama, George Armanda, Ferdio Aulia Nalar, Gusti Ayu Apriliani Ayu Dewi Pertiwi Ayu Septiana Azhari, Nazla Puteri Chyntia Devi, Made Putri Dastin Andre Dayana, Puteri Dayani, Nor Ella Debby Saputera, Debby Derlin, Ellanda Permata Desy Elisa Kismiliansari, Desy Elisa Devin, Firdi Dewi Nurdiana Dhian Ririn Lestari Dhian Ririn Lestari, Dhian Ririn Diah Puspita Rifasanti Dita Permatasari Dwi Nur Rachmah Dwi Nur Rachmah Edi Hartoyo Edi Hartoyo Edyson Edyson Edyson Edyson, Edyson Eka Yudha Rahman Erida Wydiamala Erwin Rosadi, Erwin Fachriyad, Muhammad Fahdyannoor, Fahdyannoor Fahmi, Yafi Farida Heriyani Farida Heriyani Farida Heriyani Farida Raudah, Farida Galuh Eka Suryani Hafizhah, Ghina Hardiyanti Ruslan Hayatun Nufus Hikmah Ika Darmayanta Husna Dharma Putera, Husna Dharma Ichrom Nahzi, Muhammad Yanuar Ihsanti, Shofia Hilwa Ihya Ridlo Nizomy, Ihya Ridlo Ikhwanda Angga L., Ikhwanda Angga Isnaini Isnaini Isnaini Isnaini Joharman Joharman Khatimah, Husnul Khatimah3, Husnul Lena Rosida Lie Vanny Leono Lutfia Papita Derizky Rahmayanti M. Rizki Valian Akbar, M. Rizki Valian Maharani Laillyza Apriasari Mohammad Bakhriansyah Muhammad Bayu Fernanda Muhammad, Fadil Muthmainah, Noor Nadhila Nadhila Nadya Azzahra, Nadya Nadya Salsabila Nafiah Syella, Nafiah Nafilah Syella, Nafilah Najiya Ulfa Nasution, Naulita Sari Nisa, Rohmatun Noor Rizka Yulia Rahmani Norma Sari Normaida Novianti Nur Qamariah Nurlaili Rafina Nurwafa, Nurwafa Pauline Surya Kurniati Prenggono, Muhamad Darwin Puspa Astri Sella Putra, Andifa Anugerah Putri Putri, Putri Qiptiah, Putri Mariatul Rahmah, Resvi Amalia Ridhoni, Muhammad Zaki S., Nur Almira R. Safaana, Aurora Savitri, Dwiana Siddik, Muhammad Baihaqi Silvan Juwita Siti Kaidah Sonya Esti Kholifa Sri Hayati Nufaliana, Sri Hayati Sri Widyarsi Strata Pertiwi, Strata Sukma Noor Akbar Talitha Maghfira Ramadhinta, Talitha Maghfira Thea Shagita Ulfa, Najiya Wahyuni A, Wahyuni Widya Nursantari Wydiamala, Erida Wydiamala, Erida Zafira Aisyah Putri Zohra, Noor Fathimah