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Pemanfaatan Penginderaan Jauh Dalam Penilaian Keberhasilan Reklamasi di Lahan Pasca Tambang PT. Vale Indonesia Munajat Nursaputra; Siti Halimah Larekeng; Nasri Nasri; Andi Siady Hamzah; Andi Subhan Mustari; Abdur Rahman Arif; Aris Prio Ambodo; Yohan Lawang; Andri Ardiansyah
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol. 11 No. 1 (2021): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.11.1.39-48

Abstract

Mining activities with an open system trigger land degradation which results in a decrease in land quality. The decline in land quality is related to the level of fertility and soil chemical properties, so that in general ex-mining land contains low nutrients. These problems in several mining companies that implement environmental sustainability are resolved through reclamation activities. This reclamation activity needs to be assessed, to measure the success of the mine in overcoming land degradation problems. In this study we demonstrate an assessment of the success of mine reclamation in the largest nickel mining area in South Sulawesi, using remote sensing technology. Formulation of NIR and Red bands on Sentinel 2 imagery can produce Normalized Difference Vegetation Index index. From the vegetation index value, it is known that the observed reclamation area is close to the high vegetation index value (0.7 - 0.9). This value is actually close to the vegetation index value in the surrounding natural forest, but with a low percentage of area. The distribution of the results of the assessment of plant growth in the reclamation area was lowest vegetation by 3.14%; lower vegetation by 12.15%; low vegetation by 21.53%; moderate vegetation by 14.82%; high vegetation by 25.94% and higher vegetation by 22.42% of the total reclamation area.
Penyebaran Polen Berdasarkan Analisis SSR Membuktikan Penyerbukan SITI HALIMAH LAREKENG; ISMAIL MASKROMO; AGUS PURWITO; NURHAYATI ANSHORI MATTJIK; SUDARSONO SUDARSONO
Buletin Palma Vol 16, No 1 (2015): Juni, 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v16n1.2015.77-92

Abstract

ABSTRAK Analisis paternitas digunakan untuk mengetahui pola penyebaran serbuk sari pada kelapa (Cocos nucifera L.) tipe Dalam Kalianda. Tujuan penelitian ini adalah untuk (1) mengevaluasi pola penyebaran serbuk sari dan menentukan kisaran  jarak penyebaran serbuk sari pada kelapa tipe Dalam Kalianda, (2) menentukan persentase penyerbukan silang  (outcrossing) dan penyerbukan sendiri (selfing) yang terjadi pada kelapa tipe Dalam Kalianda, dan (3) menentukan  frekuensi pola penyerbukan silang antara kelapa tipe Dalam normal (N) dengan kelapa Dalam Kopyor (K), KxN dan KxK yang terjadi pada populasi campuran antara kelapa tipe Dalam Kopyor dan kelapa Dalam normal Kalianda.  Populasi yang digunakan terdiri atas 60 pohon kelapa tipe Dalam dewasa, 21 pohon merupakan kelapa tipe Dalam berbuah normal (homozigot KK) dan 39 merupakan pohon kelapa tipe Dalam Kopyor (heterosigot Kk). Empat belas  pohon (5 pohon KK dan 9 pohon Kk) digunakan sebagai tetua betina. Sebanyak 49 progeni dipanen dari 15 induk  terpilih dan dikecambahkan untuk sumber DNA dalam analisis paternitas. Enam lokus marka SSR polimorfik, yaitu  CnCir_B12,  CnCir_86,  CnCir_87,  CnCir_56,  CnZ_51,  CnZ_18  dan  empat  lokus  marka  SNAP  polimorfik,  yaitu  CnSUS1#14,CnSUS1#3, CnWRKY6#3 dan CnWRKY19#1 digunakan untuk menentukan genotipe seluruh progeni, seluruh kandidat tetua jantan, dan semua tetua betina yang digunakan. Hasil penelitian menunjukkan bahwa  serbuk sari kelapa tipe Dalam Kalianda menyebar dengan jarak terjauh 63 m. Jarak penyebaran serbuk sari terbanyak pada jarak 40-50 m, dengan frekuensi sebesar 13 kejadian polinasi (27%). Dari 47 progeni yang dievaluasi, hanya satu (2%) progeni yang berasal dari penyerbukan sendiri (self pollination) dan 48 (98%) berasal dari penyerbukan silang. Dari  progeni hasil penyerbukan silang, 24 (49,0%) progeni teridentifikasi sebagai hasil persilangan antara induk dan tetua  jantan kelapa tipe Dalam kopyor heterosigot Kk, 11 (22,4%) sebagai hasil persilangan antara induk kelapa tipe Dalam  Kopyor heterosigot Kk dan normal homosigot KK, 10 (20,5%) sebagai hasil persilangan antara induk kelapa tipe Dalam normal homosigot KK dan Kopyor heterosigot Kk, serta 3 (6,1%) sebagai hasil persilangan antara induk dan tetua jantan tipe Dalam normal homosigot KK.Kata kunci : Kelapa Dalam Kopyor, kelapa Kopyor Kalianda, tingkat penyerbukan sendiri, tingkat penyerbukan silang. Pollen Dispersal Based on SSR Analysis Proves Kalianda to Kopyor Coconut PollinationsABSTRACT Paternity analysis was applied to determine the pattern of pollen spread among Kalianda Tall coconut (Cocos nucifera L.) in Kalianda, Lampung. The objectives of this research are to (1) evaluate patterns of pollen dispersal and ranges of pollen  spread, (2) determine percentage of outcrossing or selfing rates, and (3) determine the frequency of cross pollination  among normal (N) to kopyor (K), KxN and KxK in the mix population of Kalianda Tall coconut at Kalianda, Lampung. The population used in this study was 60 palms, consisted of 21 Kalianda Tall Normal coconuts (homozygous KK) and 39 Kalianda Tall Kopyor coconuts (Heterozygous Kk). Fourteen palms out of those were selected as female parents. Progeny arrays (49 nuts) were harvested from 15 female parents and they were germinated. The DNA was isolated from  young leaf of all adult palms and germinated coconut seedlings and they were used in paternity analysis. Six  polymorphic SSR marker loci used were CnCir_B12, CnCir_86, CnCir_87, CnCir_56, CnZ_51, CnZ_18 and the four  polymorphic SNAP markers used were CnSUS1#14, CnSUS1#3, CnWRKY6#1 and CnWRKY19#3. The markers were used to genotype all the progenies, the potential male and the female parents. Results of the experiment indicated pollen of Kalianda Tall Kopyor coconut farthest disperse was 63 m. Distance of the mostpollen dispersal was between 40-50  m,with the frequency of 13 pollination events (27%). Among the evaluated progenies, only one (2%) comes from self  pollination event and 48 (98%) comes from cross pollination. Results of the progeny evaluation also indicated 24  progenies (49.0%) are results of outcrossing among Kalianda Tall kopyor heterozygous Kk parents, 11 progenies (22.4%)  are outcrossing among kopyor heterozygous Kk female and normal homozygous KK male parents, 10 progenies (20.5%) are outcrossing among normal homozygous KK female and kopyor heterozygous Kk male parents, and 3 progenies (6.1%) are outcrossing among normal homozygous KK female and male parents.Keywords : Tall kopyor coconut, Kalianda Kopyor coconut, self polination, cross pollination rate.
A Diversity Index Model based on Spatial Analysis to Estimate High Conservation Value in a Mining Area Siti Halimah Larekeng; Munajat Nursaputra; Nasri Nasri; Andi Siady Hamzah; Andi Subhan Mustari; Abdur Rahman Arif; Aris Prio Ambodo; Yohan Lawang; Andri Ardiansyah
Forest and Society Vol. 6 No. 1 (2022): APRIL
Publisher : Forestry Faculty, Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/fs.v6i1.12919

Abstract

Large scale land-based investments have a significant impact on natural resources and environmental conditions. It is necessary to protect areas of high conservation value (HCV) within land management investments, such as the mining sector, to minimise this impact. The existence of high conservation value sites in locations with activities related to the mining sector is intended to maintain the ecological and conservation value of a mining investment area. We demonstrate a model that can identify potential high conservation value sites in mining areas using remote sensing data and spatial analysis compiled with field observation data. The research was conducted in one of the largest nickel mining areas (71,047 ha) in South Sulawesi, Indonesia. We mapped vegetation density using the normalized difference vegetation index (NDVI), calculated from Sentinel-2 imagery. We also collected biodiversity data in predetermined inventory sampling plots, which we then used to estimate species richness using the Shannon-Wiener diversity index. Using a linear regression model to compare the normalized difference vegetation index value in each sampling plot with the biodiversity value of flora and fauna, we then estimated biodiversity distribution patterns for the entire study area. We found that potential high conservation value areas (areas likely to have high biodiversity based on our regression model) covered 40,000 ha, more than half of the total concession area.
KERAGAMAN GENETIK BERDASARKAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA DARI Punica granatum L. DAN AKTIVITAS ANTIBAKTERINYA TERHADAP BAKTERI Staphyloccocus aureus DAN Pseudomonas aeruginosa. Rifdah Anggrini Zabir; Dr. Herlina Rante, M.Si., Apt; Prof. Dr. Gemini Alam, M.Si., Apt; Dr. Siti Halimah Larekeng, SP., MP.
Majalah Farmasi dan Farmakologi Vol. 26 No. 2 (2022): MFF
Publisher : Faculty of Pharmacy, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/mff.v26i2.19678

Abstract

Delima (Punica granatum L.) merupakan salah satu tanaman yang digunakan sebagai obat. P. granatum mempunyai berbagai khasiat, salah satunya sebagai antibakteri. Adanya efek antibakteri pada P. granatum karena mengandung senyawa antibakteri seperti, fenolik, flavonoid, dan tannin. Tujuan penelitian ini adalah mengetahui aktivitas antibakteri ekstrak daun P. granatum var Album, P. granatum var Nana dan P. granatum var Saveh Black terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aeroginosa ATCC 9027 serta mengetahui tingkat kekerabatan dan keragaman geneticnya. Ekstrasi yang digunakan yaitu metode maserasi menggunakan etil asetat, etanol 96, 70, dan 30%. Hasil menunjukkan ekstrak etanol 96% P. grantum dan ekstrak etanol 70% P. granatum memiliki aktivitas penghambatan terhadap bakteri S. aureus dan bakteri P. aeruginosa, sementara ekstrak etil asetat P. granatum memiliki aktivitas penghambatan terhadap bakteri S. aureus tetapi tidak memiliki memberikan aktivitas penghambatan terhadap bakteri P. aeruginosa, sedangkan ekstrak etanol 30% P.grantum daerah Gowa dan Kediri tidak memberikan aktivitas penghambatan terhadap S. aureus dan P. aeruginosa. Analisis RAPD dan dendogram menunjukkan bahwa hubungan kekerabatan antara dua kelompok P. granatum berkisar 0,65-0,72. Hal ini menunjukkan bahwa keragaman genetik antara ketiga varietas P. granatum dengan empat daerah yang berbeda cukup rendah meskipun memiliki fenotip yang berbeda.
Genetic Diversity of Duabanga moluccana Blume from Two Provenances in West Nusa Tenggara Revealed by Microsatellite markers Siti Halimah Larekeng; Yusniar Yusniar; Muh Restu; Rismawati Rismawati; Yuni Fitri Cahyaningsih; Mirza Arsiaty Arsyad; Arif Nirsatmanto
International Journal of Agriculture System VOLUME 8 ISSUE 1, JUNE 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1142.656 KB) | DOI: 10.20956/ijas.v8i1.2213

Abstract

Duabanga moluccana Blume, locally known as Rajumas, is a tree species that suitable for building materials, pulp, and plywood. The information about genetic diversity is required for the conserving of this species. Here, we elucidated the genetic diversity of D. moluccana from two provenances in West Nusa Tenggara, Indonesia. Four microsatellite markers successfully amplified 12 randomly selected samples and produced polymorphic DNA bands. Those primers were DMAG10, DMAG09, DMACAG01 and DMTCAC11. High genetic diversity was detected in the populations with 0.54 of mean expected heterozygosity (He). The genetic variation among individuals was 100%, whereas there were no genetic variations among populations and within individuals
Moderate Level of Genetic Diversity in Anthocephalus Macrophyllus Roxb, an Endemic Tree of Sulawesi and Its Implication in Conservation Siti Halimah Larekeng; Muh. Restu; Gusmiaty Gusmiaty; Syamsuddin Millang; Budirman Bachtiar
International Journal of Agriculture System VOLUME 6 ISSUE 1, JUNE 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.953 KB) | DOI: 10.20956/ijas.v6i1.1449

Abstract

Anthocephalus macrophyllus Roxb is an endemic tree to Sulawesi having high economic value. It has rarely been investigated, either in genetic population or genetic consevation aspects. The information regarding genetic diversity is very important in order to gain more understanding for breeding and conservation strategies. Genetic diversity is used as material selection of expected genotype. The study was to determine genetic diversity of A. macrophyllus from Sulawesi provenances that would be used in further development of this species. Leaf samples were collected from 108 families of A. macrophyllus belong to Sulawesi provenances. Four out of ten microsatellite markers that had high polymorphism were used for amplifying the 108 DNA samples. Results showed number of allele and mean of allele were 3 to 4 alleles and 3,5, respectively. Moreover, PIC mean of the evaluated loci was 3,7. The analysis of genetic relationship showed that the 108 families had moderate level of genetic diversity. This research suggest to establish germplasm nursery via either seed from different provenance. In situ and ex situ approaches have to be applied together for conserving genetic resources of Anthocephalus macrophyllus.
Pemetaan Potensi Pengelolaan Sumber Daya Alam dalam Perspektif Kearifan Lokal Suku Kajang Siti Halimah Larekeng; Nasri Nasri; Andi Siady Hamzah; Munajat Nursaputra; A Mujetahid; Supratman Supratman; Muh Alif KS; Asriyanni Asriyanni
Jurnal Pengabdian Dharma Wacana Vol 3, No 2 (2022): Jurnal Pengabdian Dharma Wacana
Publisher : Yayasan Pendidikan dan Kebudayaan Dharma Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37295/jpdw.v3i2.302

Abstract

Suku Kajang merupakan salah satu suku yang masih mempertahankan kearifan lokal dan nilai-nilai budaya yang ada pada suku tersebut. Kajang sendiri terbagi menjadi dua kawasan, yaitu Kajang Dalam dan Kajang Luar. Masyarakat Kajang Luar lebih modern dan berhubungan dengan perkembangan zaman saat ini. Berbeda dengan masyarakat Kajang Dalam yang masih hidup dengan cara-cara lama dengan memelihara alam agar terjaga dengan lestari. Kegiatan pengabdian masyarakat ini dilakukan untuk memotret kearifan lokal yang dipelihara di masyarakat adat Suku Kajang baik Kajang Dalam maupun Kajang Luar untuk menjaga sumber daya alam yang ada agar tetap lestari sehingga dapat dimanfaatkan oleh generasi penerusnya.  Hasil potret kearifan lokal tersebut di sosialisasikan ke masyrakat Suku Kajang yang dimaksudkan untuk memberikan informasi kepada masyarakat terkait adanya perubahan pengelolaan sumberdaya alam yang terjadi selama ini di kawasan Kajang Dalam maupun Kajang Luar.
Informasi Genetik Bitti (Vitex cofassus) di Areal Sumber Daya Genetik (ASDG) BPTH Wilayah II Sulawesi Siti Halimah Larekeng; Zulfadilah Syam; M Musriati; Rathna Paelongan; Astuti Arif; Muhammad Restu; I Iswanto
JURNAL GALUNG TROPIKA Vol 10 No 3 (2021)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v10i3.801

Abstract

Bitti (Vitex cofassus) is one of the most important tree species in Sulawesi, which is used as material for phinisi boats. The aim study was to study the genetic diversity of Bitti in the genetic resources area in Bone Regency. This study used provenance Bulukumba, Muna, Kolaka, and North Luwu proof which was analyzed based on microsatellite markers. The resulting study showed evidence that provenance Muna has a Ho value 0f 0,65 or a relatively high genetic diversity value in comparison to provenance Bulukumba, Kolaka, and North Luwu.
POLIMORFISME PRIMER RAPD PADA TANAMAN JAMBU METE ASAL TIGA KABUPATEN DI SULAWESI TENGGARA Mirza Arsiaty Arsyad; I Irwan; Dirvamena Boer; Yuni Fitri Cahyaningsih; Siti Halimah Larekeng; I Iswanto
JURNAL GALUNG TROPIKA Vol 11 No 2 (2022)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v11i2.888

Abstract

Southeast Sulawesi is one of the largest producers of cashew commodities in Indonesia, which has an essential meaning in the world of trade. Determination of the polymorphism of a primer is the first step in conducting genetic diversity studies to obtain genetic information about a species. This study aims to determine the polymorphic RAPD primers to be used in the analysis of the genetic diversity of cashew in three districts in Southeast Sulawesi. The study used 20 RAPD primers to amplify cashew DNA samples and obtained three RAPD primers (OPA-15, OPP-08, and OPC-11 primers) which showed polymorphic bands in the DNA samples used. The three RAPD primers obtained have the potential to be used in genetic studies of cashew originating from Southeast Sulawesi.
POLYMORPHISM OF SIMPLE SEQUENCE REPEAT REGIONS OF SULAWESI EBONY (DIOSPHYROS CELEBICA BAKH.) IN EXPERIMENTAL FOREST OF HASANUDDIN UNIVERSITY PROVENANCE Siti Halimah Larekeng; Muh. Restu; Gusmiaty Gusmiaty; Rismawati Rismawati
Agrotech Journal Vol 1, No 1 (2016): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v1i1.173

Abstract

Polymerase Chain Reaction (PCR)-based molecular techniques have been used to detect the polymorphism in plants. The utilization of molecular markers plays essential role in germplasm characterization and plant breeding since the information of DNA marker technology can be exchanged between laboratories and should have standard method to be reproducible. The molecular aspect has been commonly linked to DNA isolation protocol and polymorphic molecular marker, thus can be used for molecular research recommendation purposes. The objectives of this study were to evaluate the capability of microsatellite marker of Ebenaceae Family for amplifying Ebony DNA, and to determine the appropriate PCR annealing temperatures. The DNA isolation of Ebony leaves from Experimental Forest of Hasanuddin University Provenance was carried out using Genomic DNA Mini Kit (Plant) Geneaid protocol. Nine of seventeen selected primers from the Genus Diospyros were able to amplify Ebony DNA. Amplification products produced polymorphic bands with different annealing temperatures (ranged from 53 to 56°C). These nine polymorphic primers will be recommended to use for future studies in genetic diversity as well as pollen dispersal pattern analyses.
Co-Authors A Mujetahid A.R Tassakka, Asmi Citra Malina Abdul Karim Abdur Rahman Arif Adnan Adnan Agus Purwito Agus Rachmat Ahyar Ahmad Ahyar Ardat, Muh Andi Detti Yunianti Andi Detti Yunianti Andi Erwin Eka Putra Andi Prastiyo Andi Siady Hamzah Andri Ardiansyah Andri Ardiansyah Anila, Chaeria Anita Anita Arfah, Rugaiyah Andi Arif Nirsatmanto Aris Prio Ambodo Aris Prio Ambodo Arsyad, Mirza Arsiaty Aslan, Aura Aulia Asmi Citra Malina Asrianny Asrianny, Asrianny Asriyanni Asriyanni Astari Rusdin, Rista Astuti Arif Baharuddin, Ma’rifa Basse Siang Parawansa Budiaman Budiaman Budiman, Yogi Anang Budirman Bachtiar Budirman Bahtiar Dirpan, Andi Dirvamena Boer DIRVAMENA BOER, DIRVAMENA Dr. Herlina Rante, M.Si., Apt DWI SURYANTO Ekajayanti Kining ELIHAMI, ELIHAMI Fa'iq, Muhammad Feranita Haring G Gusmiaty Gusmiati Gusmiaty . Gusmiaty G. Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty, Gusmiaty Hairil Adzulyatno Hadini Hajrawati, Hajrawati Harningsih Karim Hasnah Natsir Hisein, Waode Siti Anima I Irwan I Iswanto I. Iswanto Irfandi, Rizal Irmawati . Irmawati Irmawati ISMAIL MASKROMO Ismail Maskromo Iswanto Iswanto Joeharnani Tresnati Khairana Kadriah, Ince Ayu Kurnia Kurnia M Musriati M. Yunus Sudirman Marlina Mustafa, Marlina Marwan Rajab Matjik, Nurhayati Anshori Ma’rifa Baharuddin Michely Jauwdy Stevic Mirza Arsiaty Arsyad Mirza Arsiaty Arsyad Mirza Arsiaty Arsyad Mochammad Imron Awalludin Muh Alif KS Muh Restu Muh. Farid Muh. Restu Muh. Restu Muh. Restu Muh. Restu Muhammad Bima Akzad Muhammad Fuad Anshori Muhammad Restu Muhammad Restu Munajat Nursaputra, Munajat Mustari, Andi Subhan Nasir, Anugrahandini Nasri Nasri Nawir Nawir, Nawir Negara, Satria Putra Jaya Nur Reski Immalasari NurAfni NurAfni Nurfadilah Latif NURHAYATI ANSHORI MATTJIK Nurul Muhlisah Mompewa Parawansa, Basse Siang Paulina Taba Prof. Dr. Gemini Alam, M.Si., Apt Rahayu, Rifqah Rahmat, Syaeful Ramadani, Ananda Rathna Paelongan Rayu, Surya Muh. Furqan Rehan Rehan Restu, Muhammad Rifdah Anggrini Zabir Riksfardini Annisa ERMAWAR Rismawati Rismawati Rismawati Rismawati S. Sudarsono Saidil, Saidil Sarlan, Sarlan Siti Aslamyah Sitti Nuraeni Suaib, Suaib SUDARSONO SUDARSONO Suharman Suharman, Suharman Sukriati A. Lamanda Supratman Supratman Suriati Eka Putri Syahriani Syahriani, Syahriani Syamsuddin Millang Yohan Lawang Yohan Lawang Yuni Fitri Cahyaningsih Yuni Fitri Cahyaningsih Yuni Fitri Cahyaningsih Yusniar Yusniar Zulfadilah Syam