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All Journal Jurnal Silvikultur Tropika (JST) Jurnal Natur Indonesia Tunas Geografi BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Agroteknos JFMR (Journal of Fisheries and Marine Research) JURNAL GALUNG TROPIKA Indonesian Journal of Chemistry Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) Martabe : Jurnal Pengabdian Kepada Masyarakat Forest and Society PERENNIAL Journal of Sylva Indonesiana CORD Buletin Palma Jurnal Pengabdian Dharma Wacana MALLOMO: Journal of Community Service Jurnal Pengabdian kepada Masyarakat Journal of Agriculture (JoA) International Journal of Agriculture System Perbal: Jurnal Pertanian Berkelanjutan Agrotech Journal ABDI UNISAP: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Forestry Research
SITI HALIMAH LAREKENG, SITI HALIMAH
Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Agrotech Journal

 1 
POLYMORPHISM OF SIMPLE SEQUENCE REPEAT REGIONS OF SULAWESI EBONY (DIOSPHYROS CELEBICA BAKH.) IN EXPERIMENTAL FOREST OF HASANUDDIN UNIVERSITY PROVENANCE Siti Halimah Larekeng; Muh. Restu; Gusmiaty Gusmiaty; Rismawati Rismawati
Agrotech Journal Vol 1, No 1 (2016): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v1i1.173

Abstract

Polymerase Chain Reaction (PCR)-based molecular techniques have been used to detect the polymorphism in plants. The utilization of molecular markers plays essential role in germplasm characterization and plant breeding since the information of DNA marker technology can be exchanged between laboratories and should have standard method to be reproducible. The molecular aspect has been commonly linked to DNA isolation protocol and polymorphic molecular marker, thus can be used for molecular research recommendation purposes. The objectives of this study were to evaluate the capability of microsatellite marker of Ebenaceae Family for amplifying Ebony DNA, and to determine the appropriate PCR annealing temperatures. The DNA isolation of Ebony leaves from Experimental Forest of Hasanuddin University Provenance was carried out using Genomic DNA Mini Kit (Plant) Geneaid protocol. Nine of seventeen selected primers from the Genus Diospyros were able to amplify Ebony DNA. Amplification products produced polymorphic bands with different annealing temperatures (ranged from 53 to 56°C). These nine polymorphic primers will be recommended to use for future studies in genetic diversity as well as pollen dispersal pattern analyses.
Genetic Diversity Hopea celebica an Indonesian endemic species by ISSR Marker Mirza Arsiaty Arsyad; Siti Halimah Larekeng; I. Iswanto; Muhammad Restu; Yuni Fitri Cahyaningsih; Michely Jauwdy Stevic
Agrotech Journal Vol 7, No 1 (2022): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v7i1.1737

Abstract

Indonesia is a mega-diversity country with numerous endemic plants distributed throughout its regions. An Indonesias’ island with the unique and the highest endemic plant species due to being located in the Wallace area is Sulawesi Island. Hopea celebica, an endemic species to Sulawesi Island, is currently categorized as endangered by IUCN. Here, we selected the ISSR primers suitable for the genetic study of H. celebica from Luwu and Konawe provenances and investigated their genetic diversity. Ten ISSR primers were employed in primer screening, and fifty H. celebicaindividuals were genetically analyzed for their genetic diversity. The selected ISSR primers for genetic diversity analysis were UBC 810, UBC 813, UBC 814, UBC 820, UBC 822, UBC 823, and UBC 827. The evaluated H. celebica individuals have high genetic diversity, and this information will be beneficial for designing H. celebica breeding and conservation strategies
Co-Authors A Mujetahid A.R Tassakka, Asmi Citra Malina Abda Abda Abdul Karim Abdur Rahman Arif Adnan Adnan Adnan Adnan Agus Purwito Agus Rachmat Ahyar Ahmad Ahyar Ardat, Muh Al Amanah, Hafizhah Andi Detti Yunianti Andi Detti Yunianti Andi Erwin Eka Putra Andi Prastiyo Andi Siady Hamzah Andri Ardiansyah Andri Ardiansyah Anila, Chaeria Anita Anita Arfah, Rugaiyah Andi Arif Nirsatmanto Aris Prio Ambodo Aris Prio Ambodo Arsyad, Mirza Arsiaty Aslan, Aura Aulia Asmi Citra Malina Asrianny Asrianny, Asrianny Asriyanni Asriyanni Astari Rusdin, Rista Astuti Arif Baharuddin, Ma’rifa Basse Siang Parawansa Budiaman Budiaman Budiman, Yogi Anang Budirman Bachtiar Budirman Bahtiar Dirpan, Andi Dirvamena Boer DIRVAMENA BOER, DIRVAMENA Dr. Herlina Rante, M.Si., Apt DWI SURYANTO Ekajayanti Kining ELIHAMI, ELIHAMI Fa'iq, Muhammad Feranita Haring G Gusmiaty G., Gusmiaty Gusmiati Gusmiaty . Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty, Gusmiaty Hajrawati, Hajrawati Haring, Feranita Harningsih Karim Hasnah Natsir Hisein, Waode Siti Anima I Irwan I Iswanto I. Iswanto Irfandi, Rizal Irmawati . Irmawati Irmawati ISMAIL MASKROMO Ismail Maskromo Iswanto Iswanto Joeharnani Tresnati Khairana Kadriah, Ince Ayu Kurnia Kurnia Lamanda, Sukriati A. M Musriati M. Yunus Sudirman Marlina Mustafa, Marlina Marwan Rajab Matjik, Nurhayati Anshori Ma’rifa Baharuddin Michely Jauwdy Stevic Mirza Arsiaty Arsyad Mirza Arsiaty Arsyad Mirza Arsiaty Arsyad Mochammad Imron Awalludin Muh Alif KS Muh Restu Muh. Farid Muh. Restu Muh. Restu Muh. Restu Muhammad Bima Akzad Muhammad Fuad Anshori Muhammad Restu Munajat Nursaputra, Munajat Mustari, Andi Subhan Nasir, Anugrahandini Nasri Nasri Nawir Nawir, Nawir Negara, Satria Putra Jaya Nur Reski Immalasari NurAfni NurAfni Nurfadilah Latif NURHAYATI ANSHORI MATTJIK Nurul Muhlisah Mompewa Parawansa, Basse Siang Paulina Taba Prof. Dr. Gemini Alam, M.Si., Apt Rahayu, Rifqah Rahmat, Syaeful Ramadani, Ananda Rathna Paelongan Rayu, Surya Muh. Furqan Rehan Rehan Restu, Muhammad Rifdah Anggrini Zabir Riksfardini Annisa ERMAWAR Rinaldi Sjahril, Rinaldi Rismawati Rismawati Rismawati Rismawati S. Sudarsono Saidil, Saidil Sarlan, Sarlan Siti Aslamyah Sitti Nuraeni Suaib, Suaib SUDARSONO SUDARSONO Suharman Suharman, Suharman Supratman Supratman Suriati Eka Putri Syahriani Syahriani, Syahriani Syamsuddin Millang Yohan Lawang Yohan Lawang Yuni Fitri Cahyaningsih Yuni Fitri Cahyaningsih Yuni Fitri Cahyaningsih Yusniar Yusniar Zulfadilah Syam