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All Journal HAYATI Journal of Biosciences Natural Science: Journal of Science and Technology Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Jurnal Pendidikan Matematika dan IPA AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Jurnal Biologi Universitas Andalas Indonesian Journal of Biotechnology TREUBIA JMPM (Jurnal Material dan Proses Manufaktur) Jurnal Biologi Tropis JURNAL KESEHATAN PERINTIS Majalah Kedokteran Andalas Menara Ilmu International Journal of Agricultural Sciences Sainstek : Jurnal Sains dan Teknologi Best Journal (Biology Education, Sains and Technology) Jurnal Ners Journal of Ocean, Mechanical and Aerospace -science and engineering- (JOMAse) Makara Journal of Science
Djong Hon Tjong
Universitas Andalas
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Keanekaragaman Jenis Amfibi (Ordo: Anura) di Desa Pasia Laweh, Pesisir Selatan, Sumatra Barat Yeni Gusma Yanti; Muhammad Nazri Janra; Djong Hon Tjong
Al-Kauniyah: Jurnal Biologi Vol 15, No 2 (2022): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i2.19775

Abstract

AbstrakPenelitian mengenai keanekaragaman jenis amfibi (ordo: Anura) di Desa Pasia Laweh, Kabupaten Pesisir Selatan, Sumatra Barat ini bertujuan untuk mengetahui jenis-jenis amfibi beserta indeks keanekaragamannya pada beberapa habitat yang diteliti. Penelitian dilaksanakan dari bulan Juni sampai Desember 2019 menggunakan metode survei dengan penangkapan secara aktif (visual encounter) di empat tipe habitat yaitu hutan primer, sekunder, sungai, permukiman, dan sawah, dilanjutkan dengan pengukuran morfometrik dan pendeskripsian jenis yang didapat. Hasil penelitian ini didapati 23 jenis amfibi dari enam famili, yaitu Ranidae (7 jenis), Bufonidae (4 jenis), Dicroglossidae (5 jenis), Microhylidae (3 jenis), Megophryidae (1 jenis), dan Rhacophoridae (3 jenis). Jenis Pulchrana debussyi dan Ingerophrynus claviger yang sangat sedikit catatan distribusinya juga teramati pada penelitian ini. Jenis P. debussyi, Rhacophorus nigropalmatus, dan R. reinwardtii dikoleksi dari lapangan pada fase berudu dan berhasil diidentifikasi setelah dipelihara sampai menjadi katak muda (froglet); ciri-ciri morfologi berudu dan froglet digambarkan di dalam tulisan ini. Indeks keanekaragaman jenis dari habitat-habitat amfibi yang ada di Desa Pasia Laweh mengindikasikan bahwa kawasan ini tergolong berkeaneragaman amfibi sedang.Abstract The study on the diversity of amphibian (order Anura) in Pasia Laweh Village, Pesisir Selatan Regency, West Sumatra aimed to obtain species inventory for this amphibian order as well as to reveal the diversity index at its habitats. The study was conducted from June to December 2019, using survey method where samples collected through night visual encounter technique in four habitat types; primary and secondary forest, rivers, habitation and paddy fields. Collected specimens were further measured for their morphometric parameters and taxonomically described. The study recorded 23 amphibian species classified into six families; Ranidae (7 species), Bufonidae (4 species), Dicroglossidae (5 species), Microhylidae (3 species), Megophryidae (1 species) and Rhacophoridae (3 species). Species with distribution-lacking records, namely Pulchrana debussyi and Ingerophrynus claviger, were also observed in this study. Moreover, Pulchrana debussyi, Rhacophorus nigropalmatus and R. reinwardtii were collected as tadpoles, which then reared into identifiable froglets; hence, the description on tadpoles and froglets are provided in this paper for the respective species. Diversity index from studied habitats in Pasia Laweh Village indicated that the area had moderate amphibian diversity.
Nilai Leukosit, Eritrosit dan Trombosit pada Penderita Leukemia Limfoblastik Akut Pasien Anak Rifka Rahmat; Djong Hon Tjong; Almurdi Almurdi; Meri Wulandari
JURNAL KESEHATAN PERINTIS Vol 9 No 2 (2022): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v9i2.863

Abstract

Leukemia merupakan suatu penyakit keganasan sel darah yang ditandai proliferasi leukosit secara tidak terkendali dengan manifestasi adanya sel-sel abnormal. Di Sumatera Barat penyakit ini menunjukkan prevalensi leukemia sebesar 2,4%. Penelitian ini bertujuan untuk melihat gambaran nilai Leukosit, Eritrosit dan Trombosit pada Penderita Leukemia Limfoblastik Akut (LLA) Pasien Anak di RSUP DR. M. Djamil Padang. Populasi dalam penelitan ini adalah pasien LLA anak yang menjalani kontrol di poliklinik khusus anak dan melakukan pemeriksaan darah di laboratorium sentral RSUP Dr. M Djamil yang terdiri dari 12 anak laki-laki dan 6 orang anak perempuan. Sampel adalah pasien yang memenuhi kriteria inklusi. Parameter yang diperiksa adalah leukosit, eritrosit, dan trombosit. Prinsip pemeriksaan darah adalah flow cytometri dengan teknik impedance. Dari penelitian yang telah dilakukan bahwa hasil darah pasien LLA anak memperlihatkan rerata jumlah leukosit 72,10±155,91 pada laki-laki dan 4,50±3,01 pada perempuan, jumlah eritrosit 4,19±0,84 pada laki-laki dan 3,66±0,81 pada perempuan. Selanjutnya rerata trombosit 280±138,69 pada laki-laki dan 243±1,58 pada perempuan. Dari hasil tersebut bahwa terjadi peningkatan leukosit pada laki-laki (72,10±155,91) sedangkan pada anak perempuan masih dalam rentang normal (4,50±3,01) (standar normal leukosit: 4,5-13,5).
GENETIC VARIATIONS OF TWO Anguilla SPECIES IN THE WEST COAST OF WEST SUMATRA AND MENTAWAI USING RAPD Syaifullah syaifullah; Annisya Fitri; Djong Hon Tjong; Dewi Imelda Roesma; Indra Junaidi Zakaria
Jurnal Biologi Universitas Andalas Vol 11, No 1 (2023)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.11.1.39-45.2023

Abstract

Research on the genetic variation of Anguilla spp. in the waters of the West Coast of West Sumatra was carried out from February to June 2022. This study used the RAPD (random amplified polymorphic DNA) method. In this study, two Anguilla species were found, namely A. marmorata and A. bicolor bicolor. The highest heterozygosity value of A. marmorata in the Tarusan River was 0,2018, and A. bicolor bicolor in Mentawai was 0,2340. The values of gene flow (Nm) in A. marmorata and A. bicolor bicolor were 0.7592 and 1.6318. The values of genetic differentiation (GST) in A. marmorata and A. bicolor bicolor were 0.3971 and 0.2345, respectively. This shows that the value of genetic variation of A. marmorata and A. bicolor bicolor in several rivers on the west coast of West Sumatra is low.
Phylogenetic Analysis of Cyclocheilichthys apogon and Cyclocheilichthys armatus (Fish: Cyprinidae) from West Sumatra Dewi Imelda Roesma; Djong Hon Tjong; Syaifullah; Dyta Rabbani Aidil
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.895-906

Abstract

Cyclocheilichthys apogon (Beardless Barb fish) and Cyclocheilichthys armatus (Hihgfin Barb fish) in West Sumatra is known by the local name Turiak and Catua fish, respectively, are economically important species. Reducing populations and limited biological information about these fishes make their genetic diversity and phylogenetic relationships crucial. The genetic variation was investigated by analyzing the sequencing of a part of mitochondrial DNA Cytochrome b (Cyt b) and Cytochrome oxidase-I (COI) genes of 23 individuals collected from West Sumatra populations. Phylogenetic analysis revealed the monophyletic lineage of the Cyclocheilichthys group and supported Anematichthys as the synonym of Cyclocheilichthys. C. armatus was closely related to C. janthochir and separated from C. apogon with sequences divergence were 12.7-14.0% (Cyt b) and 9.4%-10.0% (COI). C. apogon and C. armatus have low haplotype diversity and genetic diversity, respectively. Genetic diversity within and among populations with sequences divergence was 0.0-0.8% (Cyt b) and 0.0%-0.7% (COI), respectively. The low-level diversity may signify a possibility of gene flow within and among populations. The current data suggests all populations as a single stock unit. Present genetic information is important to establishing effective population management, conservation, and breeding strategies for Turiak and Catua fish.
Deteksi crypstosporidium sp. pada pasien dengan kanker kolorektal Sri Wahyuni Handayani; Nuzulia Irawati; Djong Hon Tjong; Tofrizal Tofrizal; Elli Firdamila
Majalah Kedokteran Andalas Vol 46, No 7 (2023): Supplementary December 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i7.p%p.2023

Abstract

Cryptosporidium sp. adalah parasit obligat intraseluler yang menyerang sel epitel usus. Infeksinya mengakibatkan diare, malnutrisi, dehidrasi dan cedera usus terutama pada orang dengan gangguan imunitas. Tingginya infeksi Cryptosporidium pada pasien HIV-AIDS pada penelitian  sebelumnya menandakan sumber infeksi yang tinggi di lingkungan. Cryptosporidium dilaporkan memiliki hubungan dengan kanker kolorektal baru-baru ini. Tujuan: tujuan penelitian ini adalah untuk mengetahui prevalensi infeksi Cryptosporidium pada pasien dengan kanker kolorektal. Metode: metode yang digunakan observasional dengan desain cross-sectional study pada 43 pasien kanker kolorektal yang sesuai dengan kriteria yaitu pasien yang baru didiagnosis dan belum mendapatkan terapi onkologis. Pengumpulan sampel feses dilakukan dari bulan April hingga Agustus 2023. Pemeriksaan ookista dilakukan secara mikroskopik dengan pewarnaan tahan asam modifikasi ziehl neelsen. Hasil: hasil penelitian didapatkan 46,5% (20/43) pasien positif terinfeksi Cryptosporidium. Kesimpulan: kesimpulan hasil penelitian, infeksi Cryptosporidium tinggi pada pasien kanker kolorektal, terutama pada lokasi kanker rektum. Penelitian lebih lanjut untuk mengetahui spesies Cryptosporidium diperlukan untuk mengetahui  rute penularan dan sumber infeksi.
Elimination of ineffective inorganic salt component in medium for indole‐3‐acetic acid synthesis by Serratia plymuthica UBCF_13 and its effect on the growth of chili seedlings Liza Aulia Yusfi; Djong Hon Tjong; Irawati Chaniago; Muhamad Irsyad; Jamsari Jamsari
Indonesian Journal of Biotechnology Vol 29, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.88774

Abstract

Indole‐3‐acetic acid (IAA) is an essential phytohormone that controls a variety of plant growth mechanisms. Bacteria can produce IAA to stimulate plant growth, with its production influenced by the culture conditions. Serratia plymuthica UBCF_13 is recognized as an IAA‐producing bacterium, exhibiting maximum IAA production in a yeast medium comprising yeast extract, sucrose, K2HPO4, MgSO4, NaCl, and CaCO3. However, prior studies optimizing individual inorganic salt components indicated minimal impact on IAA synthesis within this medium. This study aimed to eliminate the unnecessary inorganic salt components and the medium was then applied to investigate the IAA biosynthesis pathway and the plant growth‐promoting assay. The elimination assay consisted of yeast sucrose medium devoid of K2HPO4, MgSO4, NaCl, or CaCO3, and yeast sucrose medium containing only MgSO4 and CaCO3. Various indole compounds were then added to the revised medium composition to investigate the IAA biosynthesis pathway of UBCF_13 using high‐performance liquid chromatography (HPLC). Furthermore, the effect of UBCF_13 culture supernatant, cultivated in the new medium, on chili plant growth was evaluated. The highest IAA production (138.8 µg/mL) was observed in the yeast sucrose with CaCO3 and MgSO4 (elimination of K2HPO4 and NaCl). The presence of indole‐3‐acetamide (IAM) compound from the medium extracts, supplemented with multiple indole compounds, revealed that UBCF_13 may use the IAM pathway. The application of UBCF_13 supernatant enhanced the shoot, root length, fresh weight, and germination time of chili seeds by 37.7%, 49.3%, 204.3%, and 38.6%, respectively. This study demonstrated that eliminating K2HPO4 and NaCl provided a new culture medium composition conducive to IAA production by UBCF_13. Moreover, the UBCF_13 extract has the potential to promote plant growth.
HUBUNGAN FILOGENETIK SPESIES LIMNONECTES (RANIDAE: AMPHIBIA) ASAL SUMATERA BARAT DAN ASAL ASIA TENGGARA BERDASARKAN GEN 16S RIBOSOMAL RNA Tjong, Djong Hon; Iskandar, Djoko T.; Gusman, David
Makara Journal of Science Vol. 14, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Phylogentic Relationship among Limnonectes (Ranidae: Amphibia) found in West Sumatra with Other Species from South East Asia based on the based on the 16S rRNA Gen. The objective of this research was to study the phylogenetic relationship among Limnonectes species found in West Sumatra and with other species from South East Asia based on the partial DNA sequences16S rRNA sequences. DNA sequences were aligned using ClustalX version 1.64b, and the phylogenetic relationship within samples were analyzed using PHYLIP version 3.5c program. The alignment showed that, from 805 sites, there are 250 parsimony informative polymorphism sites. The phylogenetic tree showed that all of the Limnonectes spesies were divided in two clusters, the L. blythii complex and L. kuhlii complex. L. kuhlii and L. sp1 clustered into L. kuhlii complex and L. shomponerum and L. macrodon were clustered to L. blythii complex. This result showed that L. kuhlii and L. blythii are species complexes that are actually constituted of several species.
Regeneration of Cattleya Amazing Thailand in ½ MS Medium with The Addition of Several Concentration of PGR and Coconut Water Asnul Fitria; Zozy Aneloi Noli; Djong Hon Tjong
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6067

Abstract

One of the Cattleya species with aesthetic and commercial value is Cattleya Amazing Thailand. An efficient protocol with a high rate of plantlet regeneration is through in vitro culture. Plantlet regeneration is affected by the addition of a Plant Growth Regulator (PGR). Auxin and cytokinin (2,4-D and BAP) combined with PGR from organic material such as coconut water can increase the growth of leave and roots in plantlet regeneration. The research aims to determine the best PGRs combination in regenerating the  Cattleya Amazing Thailand orchid in vitro. The research used the Completely Randomized Design (CRD) experimental method for five treatments. The parameter are survival rate, number of leave, and number of roots. Survival rate was analyzed descriptively and both of number of leave and roots were analyzed statistically. All treatments can form 100% plantlets on survival rate parameter. The treatment of 0.1 mg/L 2,4-D + 1 mg/L BAP gave significantly different results in the number of leaf (9.0) and was not significantly different in the number of root. 0.1 mg/L 2,4-D + 1 mg/L BAP is the best combination for in vitro regeneration of the Cattleya Amazing Thailand orchid. 
Authentication of Barbonymus spp. From Lake Singkarak Using DNA Barcoding: Authentication of Barbonymus spp. From Lake Singkarak using DNA Barcoding Salis, Viola Mutiara; Roesma, Dewi Imelda; Tjong, Djong Hon; Syaifullah; Aadrean; Dahelmi
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.15

Abstract

The local community distinguishes between Barbonymus belinka (Balingka) and Barbonymus schwanefeldii (Kapiek) in Lake Singkarak based on size due to the morphological similarities between the two species. From previous reports, B. belinka (Balingka) is a fish endemic to Lake Singkarak, West Sumatra, while B. schwanefeldii has a wider distribution, including Sumatra, Kalimantan, and Java. Consequently, molecular identification is necessary to discern between the species and to understand the DNA barcode characteristics of fish belonging to the genus Barbonymus in Lake Singkarak. One molecular technique utilized for species identification is DNA barcoding, which focuses on the COI (Cytochrome Oxidase Subunit I) gene. Liver tissue samples from Balingka and Kapiek fish from Lake Singkarak were used in the study. Based on 585 bp of COI gene sequences and 30 comparison sequences from BOLD system and GenBank NCBI, seven samples from Lake Singkarak show a genetic distance of 0–1.2% from B. schwanefeldii populations elsewhere, with 15 differing nucleotide bases. Moreover, samples from Lake Singkarak show a genetic distance of 7.7–8.2% from B. belinka in the BOLD system from Aceh, with 42 differing nucleotide bases. Furthermore, two specific bases are present in B. schwanefeldii from Lake Singkarak. Based on the results of this research, it is known that all samples from Lake Singkarak, including Balingka and Kapiek, belong to the same species, namely B. schwanefeldii.
Keterbatasan Data Molekuler Pada Burung Kakatua Raja ((Probosciger aterrimus Gmelin, 1788): Dampak pada Konservasi Rahmadina, Shovinda; Tjong, Djong Hon; Dharmayanthi, Anik Budhi
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10974

Abstract

Probosciger aterrimus atau kakatua raja merupakan spesies burung kakatua yang menghadapi ancaman konservasi akibat deforestasi, perdagangan ilegal, dan tingkat reproduksi yang rendah. Kajian ini bertujuan untuk menelaah literatur terkait genetika molekuler guna memahami struktur populasi serta implikasi konservasinya. Studi terdahulu menunjukkan adanya perbedaan genetik yang signifikan antarpopulasi, yang dapat berdampak pada strategi konservasi, terutama dalam translokasi dan program pemuliaan berbasis konservasi. Namun, keterbatasan data molekuler masih menjadi tantangan dalam pengelolaan spesies ini. Selain itu, kurangnya pemetaan genetik dapat meningkatkan risiko hilangnya adaptasi lokal atau outbreeding depression akibat pencampuran populasi yang tidak sesuai. Oleh karena itu, penelitian lebih lanjut menggunakan teknologi genomik seperti Next-Generation Sequencing (NGS) dan Single Nucleotide Polymorphisms (SNPs) diperlukan untuk memastikan strategi konservasi yang lebih efektif. Kajian ini menekankan pentingnya pendekatan berbasis genetika dalam perlindungan jangka panjang P. aterrimus serta perancangan kebijakan konservasi yang lebih akurat
Co-Authors - Jumawita - Syaifullah Aadrean Aidil, Dyta Rabbani Aldino Fauzil Fanani Almurdi Almurdi AMIR HAMIDY Amir Hamidy Anggraini, Debie Annisya Fitri Anthoni Agustien Anugrah Viona Agesi Arum Setiawan Asnul Fitria Aurora, Dhea Apriano Dahelmi Dahelmi Dahelmi David Gusman Dewi Imelda Roesma Dharmayanthi, Anik Budhi Dian Juliadmi Djoko T. Iskandar Djoko T. Iskandar, Djoko T. Dwitya Elvira, Dwitya Dyta Rabbani Aidil Elli Firdamila fachrul reza, fachrul Fauzan . Feskaharny Alamsjah Firdamila, Elli Fuji Astuti Febria G Gunawarman, G Gusman, David Hadi Addaha, Hadi Hadi Kurniawan Henny Herwina Hirowati Ali Hirowati Ali, Hirowati Ida Ayu Putu Sri Widnyani Ilhamdi, Ilhamdi Indra Junaidi Zakaria Irawati Chaniago Irvan Fadli Wanda Jamsari Jamsari Janra, Muhammad Nazri Jon Affi, Jon Juliadmi, Dian Kevin Origia Kevin Origia Kharisma Putra Liza Aulia Yusfi Mahmudul Hasan Mahmudul Hasan, Mahmudul Marlina Marlina Masayuki Sumida Masayuki Sumida, Masayuki Maulana, Imron Melinda, Annisa Menkher Manjas Meri Wulandari Muhamad Irsyad Muhammad Silmi Nelmi Fitria Netty Marusin Netty Marusin Nia Kurniawan Nia Kurniawan Nofrita Nofrita Nofrita Nofrita Nurul Aida Nurvalinda, Nurvalinda Nuswantoro, Nuzul Ficky Nuzulia Irawati Oktaviana, Dili Padilla, Putri Rahma Prawira, Furqan Dwiki Lintang Putri, Emilya Putri, Fanny Adhy Rahmadina, Shovinda Ravelino Nesty Resti Rahayu Ridho Hendrikos Rifka Rahmat Riska Mayori Rita Permatasari Rivi Hamdani Rizaldi Rizaldi Rizia Irsa Salis, Viola Mutiara Saputri, Rahmi Agu Silvia Indra Sisca Dwi Yarni Sri Wahyuni Handayani Sri Wahyuni Handayani, Sri Wahyuni Syaifullah Syaifullah Syaifullah Syaifullah Syaifullah Syandri, Hafrijal Takeshi Igawa Takeshi Igawa, Takeshi Tesri Maideliza Tofrizal Wilson Novarino Yarni, Sisca Dwi Yelvita Sari Yeni Gusma Yanti Yuliarni Syafrita Yurike Nova Edlin Zil Fadhillah Rahma Zozy Aneloi Noli