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All Journal HAYATI Journal of Biosciences Natural Science: Journal of Science and Technology Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pendidikan Matematika dan IPA AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Jurnal Biologi Universitas Andalas Indonesian Journal of Biotechnology JMPM (Jurnal Material dan Proses Manufaktur) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JURNAL KESEHATAN PERINTIS Majalah Kedokteran Andalas Menara Ilmu International Journal of Agricultural Sciences Jurnal Pembelajaran dan Biologi Nukleus Sainstek : Jurnal Sains dan Teknologi Best Journal (Biology Education, Sains and Technology) Jurnal Ners Journal of Ocean, Mechanical and Aerospace -science and engineering- (JOMAse) Makara Journal of Science Treubia Biodiversitas Journal of Biological Diversity
Djong Hon Tjong
Universitas Andalas
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Analysis Morphological Characters of Chalcorana chalconota (Schelgel, 1837) at Soraya and Ketambe Research Stations, Aceh Fanny Lestari; Essy Harnelly; Djong Hon Tjong
Jurnal Penelitian Pendidikan IPA Vol 12 No 4 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i4.14694

Abstract

Chalcorana is a genus of amphibians that consists of species complexes which are difficult to distinguish morphologically. The difficulty in identifying species within this genus has prompted taxonomic research to utilize a more in-depth morphological approach in order to determine a more accurate taxonomic status and to examine the grouping of the Chalcorana genus originating from two different locations. This study used a principal component analysis (PCA) approach by measuring morphological characters. Based on the results of this study, it was found that different locations did not affect the variation in morphological characters, and therefore, no grouping was formed based on locality
Design and Experimental Validation of qPCR Primer for Drought-Responsive CaHsfA2 Gene in Capsicum annuum for Sustainable Crop Production Suwirmen; Zozy Aneloi Noli; Anthonie Agustien; Djong Hon Tjong; Amanda Nurhafitri
Jurnal Penelitian Pendidikan IPA Vol 12 No 5 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i5.14897

Abstract

Drought stress severely reduces plant productivity by disrupting photosynthesis and cellular homeostasis. Plants respond through regulatory networks involving stress-responsive genes, including heat shock proteins (HSPs), regulated by heat shock transcription factors (HSFs). Among these, HsfA2 plays an important role in abiotic stress tolerance, including drought. Although CaHsfA2 expression has been reported in Capsicum annuum, validated primers for quantitative PCR (qPCR) analysis remain limited. This study aimed to design and validate specific primers for CaHsfA2 expression analysis. Primer candidates were designed from sequence NM_001324561.1 using Primer-BLAST and evaluated based on length, GC content, melting temperature (Tm), and self-complementarity. Total RNA was extracted from chili roots subjected to drought stress (50% field capacity) and nanopriming with Padina minor, followed by cDNA synthesis, gradient PCR and agarose gel electrophoresis.. Secondary structures were analyzed using OligoAnalyzer. Of ten primer pairs, four met the criteria, and primer set 1 (1_CaHsfA2) showed optimal characteristics (%GC 55%, Tm 60°C, product length 190 bp) and minimal secondary structure potential (ΔG > −9 kcal/mol). Gradient PCR (55.3–59.2°C) produced a single band (~193 bp), consistent with the predicted size (190 bp), with an optimal annealing temperature of 57.3°C, confirming its specificity and suitability for qPCR-based gene expression analysis under drought conditions.
Sex-Specific Bands in Dioecious Plant Jernang Rattan (Daemonorops draco) Based on RAPD Markers Amanda Nurhafitri; Mansyurdin Mansyurdin; Djong Hon Tjong; Syamsuardi Syamsuardi; Revis Asra; Muhammad Idris
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.9059

Abstract

Background: Daemonorops draco (Willd.) Blume, or dragon’s blood rattan, is a dioecious species that produces high-value resin but is increasingly threatened by habitat degradation. Effective cultivation and conservation require early identification of male and female plants, which remains challenging at the seedling stage. Although RAPD markers have been used for sex identification in dioecious plants, studies on D. draco are still limited. Therefore, this study aims to screen sex-specific bands in D. draco using the RAPD approach. Methodology: Samples of six male and six female individuals collected from Jambi were analyzed, with DNA isolated using a modified CTAB protocol and amplified using 44 RAPD primers. The products were separated by 2% agarose gel electrophoresis. Bands were considered sex-specific if they consistently appeared only in one sex and were absent in the other. Findings: Out of the 44 primers tested, 39 successfully amplified D. draco DNA, generating a total of 662 bands. Among them, primer OPA-14 (455 bp) and OPC-19 (310 bp) produced male-specific bands that were consistently present in all male individuals, while primer OPB-10 (850 bp) generated a female-specific band detected in all female individuals. The male-specific 455 bp band from OPA-14 and 310 bp from OPC-19, and the female-specific 850 bp band from OPB-10 were identified. These bands are recommended for development into SCAR markers for early sex identification in D. draco. Contributions: The findings support the provision of male and female seedlings to enhance conservation and cultivation programs
In-silico Design and Testing of Tetra-Primer Arms-PCR for SNPs-Based Identification of Sumatran Tiger (Panthera tigris sumatrae): ARMS-PCR SNP Identification of Sumatran Tiger Asrori, Ikrima; Novarino, Wilson; Tjong, Djong Hon; Roesma, Dewi Imelda
Journal of Tropical Life Science Vol. 16 No. 01 (2026)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.16.01.03

Abstract

The Sumatran tiger (Panthera tigris sumatrae) is a subspecies of tiger classified as critically endangered by the International Union for Conservation of Nature (IUCN) due to poaching and illegal trade. In the law enforcement process, it is very important to ensure that the sample is indeed from a Sumatran tiger. Seized samples from illegal trade cannot be identified based on their morphology because they have been degraded and processed into other forms. Previous studies have reported the use of molecular markers for Sumatran tiger identification, which involve lengthy procedures and analyses. Therefore, for a more effective identification process, faster and more accurate techniques are needed. This study aims to design tetra primers for the identification of Sumatran tiger subspecies, particularly from incomplete confiscated samples, using the Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR) technique based on Single Nucleotide Polymorphism (SNP) markers. The primers were designed using the Primer1 application and validated in silico with CloneManager and NCBI BLAST, as well as through direct testing on Sumatran tiger blood samples. The design results showed one pair of primers with optimal specifications, producing G allele fragments (452 bp), A allele fragments (408 bp), and internal control fragments (819 bp). Specificity testing results indicated that all samples were Sumatran tigers, with amplification bands at 452 bp and 819 bp. These results demonstrate that the developed tetra-primer ARMS-PCR assay provides a rapid and practical molecular approach for identifying Sumatran tiger samples, particularly for forensic verification of confiscated wildlife materials.
Variasi Morfologi Fejervarya cancrivora Gravenhorst, 1892 (Anura: Dicroglossidae) di Sumatra Barat Nadyatul Khaira Huda; Djong Hon Tjong; Dewi Imelda Roesma
BEST Journal (Biology Education, Sains and Technology) Vol 7, No 2 (2024): September 2024
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v7i2.9687

Abstract

Bukit Barisan sebagai barrier ekologi yang dapat membatasi migrasi dan aliran gen khususnya amfibi. Penelitian ini bertujuan untuk melacak variasi morfologi Fejervarya cancrivora di Sumatra Barat berdasarkan morfometrik. Total 120 individu F. cancrivora dari enam populasi meliputi Padang, Pariaman dan Pesisir Selatan sebagai bagian barat Bukit Barisan. Pasaman, Limapuluh Kota dan Sijunjung sebagai bagian timur Bukit Barisan. Hasil analisis data morfometrik didapatkan 13 karakter yang bervariasi pada individu-individu jantan yaitu SL, EN, TEL, MN, HAL, FAL, HLL, THIGHL, TL, FOL, TFOL, 3FL and 1FL.dan 16 karakter bervariasi pada individu-individu betina yaitu SVL, HL, MSL, NS, SL, TEL, MN, MFE, IN, IOD, FAL, THIGHL, FOL, 4TL, IMTL and 1 TL. Berdasarkan hasil analisis PCA menunjukkan keenam plot saling tumpang tindih. Dapat disimpulkan bahwa meskipun terdapat karakter yang bervariasi, tidak ada perbedaan secara signifikan yang memisahkan satu populasi dengan populasi lainnya..
Genetic diversity of Tor douronensis (Pisces: Cyprinidae) in West Sumatra, Indonesia DEWI IMELDA ROESMA; DJONG HON TJONG; WARNETY MUNIR; ANUGRAH VIONA AGESI; ADA CHORNELIA
Biodiversitas Journal of Biological Diversity Vol. 18 No. 3 (2017)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d180320

Abstract

Roesma DI, Tjong DH, Munir W, Agesi AV, Chornelia A. 2017. Genetic diversity of Tor douronensis (Pisces: Cyprinidae) in West Sumatra, Indonesia. Biodiversitas 18: 1018-1025. A random amplified polymorphic DNA-polymerase chain reaction (RAPDPCR) analysis was applied to determine the genetic diversity within and among the populations of Tor douronensis in West Sumatra river system. The results provided information for proper breeding, maintenance, and conservation of T. douronensis. The highest and the lowest values of genetic diversity were the population of Batang Matur (H = 0.1571; I = 0.2411) and Batang Malalo (H = 0.0880; I = 0.1353), respectively. The value of genetic differentiation (GST) was 0.4266 while that of heterozygosity between populations (DST) and within a population (HS) was, respectively, 0.088 and 0.1184. The value of gene flow between populations (Nm) was 0.6721. Grouping of individuals in a population based Principal Coordinates Analysis (PCO) and UPGMA was in line with the genetic distance values. Matur and Malalo were the most closely related populations (0.0174) while Batang Gumanti and Batang Sinuruik were the most distantly related populations (0.1918). The genetic variation between populations was lower than the genetic variation within the population of T. douronensis and population of Matur is recommended to be used as brood stock in the procurement of stock.
Taxonomy confirmation of Puntius cf. binotatus from Gunung Tujuh Lake, Jambi, Indonesia based on Cytochrome Oxidase-I (COI) gene DEWI IMELDA ROESMA; DJONG HON TJONG; WILA KARLINA; DYTA RABBANI AIDIL
Biodiversitas Journal of Biological Diversity Vol. 20 No. 1 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200107

Abstract

Roesma D. I, Tjong D. H, Karlina W, Aidil D. R. 2019. Taxonomy confirmation of Puntius cf. binotatus from Gunung Tujuh Lake based on Cytochrome Oxidase-I (COI) gene. Biodiversitas 20: 54-60. The population of Puntius cf. binotatus Gunung Tujuh Lake in considered as an isolated population which found in the 20-30 meters depth under the water Gunung Tujuh Lake, Sumatra Island. The species often exhibit different phenotypic characters and may genetic characters may also different due to their response to habitat/ecological conditions. Previous study on P. binotatus from several locations in West Sumatra showed the complexity of the genetic and morphological characters. A molecular study using COI gene of P. cf. binotatus from Gunung Tujuh Lake in Sumatera has been conducted to determine the taxonomic status of the fish. The distinct morphological characters of Puntius cf. binotatus is one black spot in the middle of the caudal and the absent of black spot on the base of the anterior dorsal ray. The analysis on COI gene showed that the range of sequence divergences between P. cf. binotatus Gunung Tujuh Lake and other Sumatra P. cf. binotatus is 3.1-7.6% and 4.1% to Barbodes banksi. This value represents the differences at the subspecies level of Barbodes banksi. Therefore Barbodes banksi gunungtujuh is proposed as an appropriate name to P. cf. binotatus Gunung Tujuh Lake.
Phylogenetic analysis of transparent gobies in three Sumatran lakes, inferred from mitochondrial Cytochrome Oxidase I (COI) gene DEWI IMELDA ROESMA; DJONG HON TJONG; DYTA RABBANI AIDIL
Biodiversitas Journal of Biological Diversity Vol. 21 No. 1 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210107

Abstract

Abstract. Roesma DI, Tjong DH, Aidil DR. 2020. Phylogenetic analysis of transparent gobies in three Sumatran lakes, inferred from mitochondrial Cytochrome Oxidase I (COI) gene. Biodiversitas 21: 43-48. The transparent gobies fish found in three lakes in Sumatra island is known as Rinuak fish (in Maninjau Lake and Singkarak Lake, West Sumatra, Indonesia) or Badar fish (in Siais Lake, North Sumatra, Indonesia), and are morphologically very similar to the Gobiopterus brachypterus. The phylogenetic study was carried out by analyzing 619 base pairs of the mitochondrial DNA cytochrome oxidase subunit I (COI) gene in 12 fish individuals from the three lakes. Rinuak and Badar fish in three populations have four haplotypes. The sequence divergences in and between populations are very low (0.0-0.5%). This value indicates that Rinuak and Badar fish are the same species with low genetic diversity. The phylogenetic tree illustrates that this fish belongs to the group of Gobiidae and a sister taxon from G. brachypterus.
Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA: - DEWI IMELDA ROESMA; HON TJONG DJONG; MUHAMMAD NAZRI JANRA; DYTA RABBANI AIDIL
Biodiversitas Journal of Biological Diversity Vol. 22 No. 5 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220543

Abstract

Abstract. Roesma DI, Tjong DH, Janra MN, Aidil DR. 2021. Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA. Biodiversitas 22: 2794-2802. Environmental DNA (eDNA) is one of the DNA sources without directly disturbing the organisms. The eDNA potentially an effective method with next-generation sequencing (NGS) that can read all DNA in parallel at one sequential run. Maninjau Lake in West Sumatra has experienced a decline the species richness, thus monitor biodiversity with the non-invasive method is needed. This study aimed to apply eDNA for monitoring freshwater vertebrates in Maninjau Lake. The eDNA was collected from water samples using the filter membrane to DNA isolation and sequencing with the NGS technique from Illumina. The eDNA method detected 150 vertebrate individuals and consists of 92 Actinopterygii fishes, three chondrichthyes fishes, 11 amphibians, seven reptiles, ten aves, and 27 mammals. Among all individuals were detected, only 11% known present in Maninjau Lake, while the others (89%) are native species to other countries. The fishes were detected living in Maninjau Lake by the eDNA method, i.e., Cyprinidae (Carassius auratus and Cyprinus carpio), Cichlidae (Oreochromis niloticus), and Poeciliidae (Gambusia affinis and Xiphophorus maculatus). Bias results in this study to be influenced by various factors i.e., possibility of contamination, poor DNA quality, the bias results during molecular processes, and not available the complete sequences databases to assigned into the correct taxa, especially fish species in Maninjau Lake. The eDNA method for monitoring biodiversity should consider the increase of sampling sites, replication samples, and the availability of sequences database in Genbank, NCBI
Sumatran tiger identification and phylogenetic analysis based on the CO1 gene: Molecular forensic application ASHRIFURRAHMAN ASHRIFURRAHMAN; SARUEDI SIMAMORA; RUSDIYAN RITONGA; WILSON NOVARINO; DJONG HON TJONG; RIZALDI RIZALDI; SYAIFULLAH SYAIFULLAH; DEWI IMELDA ROESMA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 4 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230410

Abstract

Abstract. Ashrifurrahman, Simamora S, Ritonga R, Novarino W, Tjong DH, Rizaldi, Syaifullah, Roesma DI. 2022. Sumatran tiger identification and phylogenetic analysis based on the CO1 gene: molecular forensic application. Biodiversitas 23: 1788-1794. Wild animal hunting, especially in the Sumatran tiger (Panthera tigris sumatrae), has been caused the population decline. Regulation and law enforcement have been implemented even though it does not affect optimal because of the trickery of poachers and illegal traders. Sometimes, the evidence of P. t. sumatrae derivative products, for example, bones, nails, skins, hair, and other body parts, cannot be properly identified to raise the cases. However, genetic markers, such as the CO1 gene, have successfully identified illegal trafficking samples. This study used 20 samples, consisting of seven samples (four preserved hairs, two claws, one bone) that were suspected of P. t. sumatrae collected from illegal wildlife trade cases in West Sumatra, Indonesia. Other thirteen samples were twelve blood and one hair of P. t. sumatrae samples were collected from the Dharmasraya Sumatran Tiger Rehabilitation Center (PR-HSD). All samples were isolated, Polymerase Chain Reaction (PCR), sequenced, and 999 base pairs (bp) of the CO1 gene sequences were analyzed. In addition, National Center for Biotechnology Information (NCBI) data sequences including two P. t. sumatrae sequences, four P. t. altaica sequences, one P. t. amoyensis sequence, one P. t. corbetti sequence, three P. pardus sequences, and one Felis catus sequence were collected for comparison and supporting data. The result confirmed that all samples in this study were P. t. sumatrae. We determined those depending on similarity value which was 99.60%-99.70% with P. tigris reference sequence (NC_010642.1) and 99.90%-100% with P. t. sumatrae (JF357969.1). Phylogenetic analysis supported species identification with average intraspecies sequence divergence was 0 to 0.4% and presented the monophyletic group. This study was the first and most recent report to use seized samples to identify P. t. sumatrae based on the CO1 gene in West Sumatra, Indonesia.
Co-Authors - Jumawita - Syaifullah Aadrean Aadrean Aadrean ADA CHORNELIA Aidil, Dyta Rabbani Aldino Fauzil Fanani Almurdi Almurdi Amanda Nurhafitri AMIR HAMIDY Amir Hamidy Amir Hamidy ANDRI SAPUTRA Anggraini, Debie Anik Budhi Dharmayanthi Annisa Farma Annisa Khaira Annisya Fitri Anthoni Agustien Anthonie Agustien Anugrah Viona Agesi ANUGRAH VIONA AGESI Arum Setiawan Ashrifurrahman Ashrifurrahman Asnul Fitria Asrori, Ikrima Aurora, Dhea Apriano Cynthia Ericca Dahelmi Dahelmi Dahelmi David Gusman Dewi Imelda Roesma Dewi Roesma Dharmayanthi, Anik Budhi Dian Juliadmi Djoko T. Iskandar Djoko T. Iskandar, Djoko T. Dwitya Elvira Dyta Aidil DYTA RABBANI AIDIL DYTA RABBANI AIDIL DYTA RABBANI AIDIL DYTA RABBANI AIDIL DYTA RABBANI AIDIL DYTA RABBANI AIDIL Dyta Rabbani Aidil Elli Firdamila Endang Purwati RN Essy Harnelly fachrul reza, fachrul Fanny Adhy Putri Fanny Lestari Fatriza, Ayu Septia Fauzan . Feskaharny Alamsjah Firdamila, Elli FURQAN DWIKI LINTANG PRAWIRA FURQAN DWIKI LINTANG PRAWIRA G Gunawarman, G Gusman, David Hadi Addaha, Hadi Hadi Kurniawan Henny Herwina Hirowati Ali Hirowati Ali, Hirowati Huda, Nadyatul Khaira Ida Ayu Putu Sri Widnyani IKRIMA ASRORI IKRIMA ASRORI Ilhamdi, Ilhamdi Indra Junaidi Zakaria INDRI LESTARI Irawati Chaniago Irfan Suliansyah Irvan Fadli Wanda Jamsari Jamsari Janra, Muhammad Nazri Jon Affi, Jon Juliadmi, Dian Kevin Origia Kevin Origia Kharisma Putra Liza Aulia Yusfi M. Idris Mahmudul Hasan Mahmudul Hasan Mahmudul Hasan, Mahmudul Mansyurdin Marlina Marlina Masayuki Sumida Masayuki Sumida Masayuki Sumida, Masayuki Maulana, Imron Melinda, Annisa Menkher Manjas Meri Wulandari Muhamad Irsyad Muhammad Janra Muhammad Idris Muhammad Silmi Nadyatul Khaira Huda Nelmi Fitria Netty Marusin Netty Marusin Nia Kurniawan Nia Kurniawan Nia Kurniawan Nofrita Nofrita Nofrita Nofrita Nurhafitri, Amanda Nurul Aida Nurvalinda Nurvalinda Nuswantoro, Nuzul Ficky Nuzulia Irawati Oktaviana, Dili Padilla, Putri Rahma PATRICK FLAGGELLATA Prawira, Furqan Dwiki Lintang Putri, Emilya Rahmadina, Shovinda Ravelino Nesty Resti Rahayu Revis Asra Ridho Hendrikos Rifka Rahmat Riska Mayori Rita Permatasari Rivi Hamdani Rizaldi Rizaldi Rizia Irsa RUSDIYAN RITONGA Salis, Viola Mutiara Saputri, Rahmi Agu SARUEDI SIMAMORA Shovinda Rahmadina Silvia Indra Sisca Dwi Yarni Sri Wahyuni Handayani Sri Wahyuni Handayani, Sri Wahyuni Suwirmen, Suwirmen Syaifullah SYAIFULLAH SYAIFULLAH Syaifullah Syaifullah Syaifullah Syaifullah SYAIFULLAH SYAIFULLAH SYAIFULLAH SYAIFULLAH SYAIFULLAH SYAIFULLAH Syaifullah Syaifullah SYAIFULLAH SYAIFULLAH Syamsuardi Syamsuardi Syandri, Hafrijal Takeshi Igawa Takeshi Igawa Takeshi Igawa, Takeshi Tesri Maideliza Tesri Maideliza Tofrizal UMMI KURNIA PUTRI VIOLA MUTIARA SALIS VIOLA MUTIARA SALIS WARNETY MUNIR WILA KARLINA Wilson Novarino Yarni, Sisca Dwi Yelvita Sari Yeni Gusma Yanti YOLI ZULFANEDI Yuliarni Syafrita Yurike Nova Edlin Zil Fadhillah Rahma Zozy Aneloi Noli Zulkarnain, Alivia