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All Journal HAYATI Journal of Biosciences Natural Science: Journal of Science and Technology Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pendidikan Matematika dan IPA AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Jurnal Biologi Universitas Andalas Indonesian Journal of Biotechnology JMPM (Jurnal Material dan Proses Manufaktur) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JURNAL KESEHATAN PERINTIS Majalah Kedokteran Andalas Menara Ilmu International Journal of Agricultural Sciences Jurnal Pembelajaran dan Biologi Nukleus Sainstek : Jurnal Sains dan Teknologi Best Journal (Biology Education, Sains and Technology) Jurnal Ners Journal of Ocean, Mechanical and Aerospace -science and engineering- (JOMAse) Makara Journal of Science
Djong Hon Tjong
Universitas Andalas
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Design and Experimental Validation of qPCR Primer for Drought-Responsive CaHsfA2 Gene in Capsicum annuum for Sustainable Crop Production Suwirmen; Zozy Aneloi Noli; Anthonie Agustien; Djong Hon Tjong; Amanda Nurhafitri
Jurnal Penelitian Pendidikan IPA Vol 12 No 5 (2026): In Progress
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i5.14897

Abstract

Drought stress severely reduces plant productivity by disrupting photosynthesis and cellular homeostasis. Plants respond through regulatory networks involving stress-responsive genes, including heat shock proteins (HSPs), regulated by heat shock transcription factors (HSFs). Among these, HsfA2 plays an important role in abiotic stress tolerance, including drought. Although CaHsfA2 expression has been reported in Capsicum annuum, validated primers for quantitative PCR (qPCR) analysis remain limited. This study aimed to design and validate specific primers for CaHsfA2 expression analysis. Primer candidates were designed from sequence NM_001324561.1 using Primer-BLAST and evaluated based on length, GC content, melting temperature (Tm), and self-complementarity. Total RNA was extracted from chili roots subjected to drought stress (50% field capacity) and nanopriming with Padina minor, followed by cDNA synthesis, gradient PCR and agarose gel electrophoresis.. Secondary structures were analyzed using OligoAnalyzer. Of ten primer pairs, four met the criteria, and primer set 1 (1_CaHsfA2) showed optimal characteristics (%GC 55%, Tm 60°C, product length 190 bp) and minimal secondary structure potential (ΔG > −9 kcal/mol). Gradient PCR (55.3–59.2°C) produced a single band (~193 bp), consistent with the predicted size (190 bp), with an optimal annealing temperature of 57.3°C, confirming its specificity and suitability for qPCR-based gene expression analysis under drought conditions.
Sex-Specific Bands in Dioecious Plant Jernang Rattan (Daemonorops draco) Based on RAPD Markers Amanda Nurhafitri; Mansyurdin Mansyurdin; Djong Hon Tjong; Syamsuardi Syamsuardi; Revis Asra; Muhammad Idris
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.9059

Abstract

Background: Daemonorops draco (Willd.) Blume, or dragon’s blood rattan, is a dioecious species that produces high-value resin but is increasingly threatened by habitat degradation. Effective cultivation and conservation require early identification of male and female plants, which remains challenging at the seedling stage. Although RAPD markers have been used for sex identification in dioecious plants, studies on D. draco are still limited. Therefore, this study aims to screen sex-specific bands in D. draco using the RAPD approach. Methodology: Samples of six male and six female individuals collected from Jambi were analyzed, with DNA isolated using a modified CTAB protocol and amplified using 44 RAPD primers. The products were separated by 2% agarose gel electrophoresis. Bands were considered sex-specific if they consistently appeared only in one sex and were absent in the other. Findings: Out of the 44 primers tested, 39 successfully amplified D. draco DNA, generating a total of 662 bands. Among them, primer OPA-14 (455 bp) and OPC-19 (310 bp) produced male-specific bands that were consistently present in all male individuals, while primer OPB-10 (850 bp) generated a female-specific band detected in all female individuals. The male-specific 455 bp band from OPA-14 and 310 bp from OPC-19, and the female-specific 850 bp band from OPB-10 were identified. These bands are recommended for development into SCAR markers for early sex identification in D. draco. Contributions: The findings support the provision of male and female seedlings to enhance conservation and cultivation programs
In-silico Design and Testing of Tetra-Primer Arms-PCR for SNPs-Based Identification of Sumatran Tiger (Panthera tigris sumatrae): ARMS-PCR SNP Identification of Sumatran Tiger Asrori, Ikrima; Novarino, Wilson; Tjong, Djong Hon; Roesma, Dewi Imelda
Journal of Tropical Life Science Vol. 16 No. 01 (2026)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.16.01.03

Abstract

The Sumatran tiger (Panthera tigris sumatrae) is a subspecies of tiger classified as critically endangered by the International Union for Conservation of Nature (IUCN) due to poaching and illegal trade. In the law enforcement process, it is very important to ensure that the sample is indeed from a Sumatran tiger. Seized samples from illegal trade cannot be identified based on their morphology because they have been degraded and processed into other forms. Previous studies have reported the use of molecular markers for Sumatran tiger identification, which involve lengthy procedures and analyses. Therefore, for a more effective identification process, faster and more accurate techniques are needed. This study aims to design tetra primers for the identification of Sumatran tiger subspecies, particularly from incomplete confiscated samples, using the Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR) technique based on Single Nucleotide Polymorphism (SNP) markers. The primers were designed using the Primer1 application and validated in silico with CloneManager and NCBI BLAST, as well as through direct testing on Sumatran tiger blood samples. The design results showed one pair of primers with optimal specifications, producing G allele fragments (452 bp), A allele fragments (408 bp), and internal control fragments (819 bp). Specificity testing results indicated that all samples were Sumatran tigers, with amplification bands at 452 bp and 819 bp. These results demonstrate that the developed tetra-primer ARMS-PCR assay provides a rapid and practical molecular approach for identifying Sumatran tiger samples, particularly for forensic verification of confiscated wildlife materials.
Co-Authors - Jumawita - Syaifullah . Mansyurdin Aadrean Aidil, Dyta Rabbani Aldino Fauzil Fanani Almurdi Almurdi Amanda Nurhafitri AMIR HAMIDY Amir Hamidy Anggraini, Debie Annisa Farma Annisa Khaira Annisya Fitri Anthoni Agustien Anthonie Agustien Anugrah Viona Agesi Arum Setiawan Asnul Fitria Asrori, Ikrima Aurora, Dhea Apriano Dahelmi Dahelmi Dahelmi David Gusman Dewi Imelda Roesma Dharmayanthi, Anik Budhi Dian Juliadmi Djoko T. Iskandar Djoko T. Iskandar, Djoko T. Dwitya Elvira Dyta Rabbani Aidil Elli Firdamila Endang Purwati RN Essy Harnelly fachrul reza, fachrul Fanny Adhy Putri Fanny Lestari Fauzan . Feskaharny Alamsjah Firdamila, Elli Fuji Astuti Febria G Gunawarman, G Gusman, David Hadi Addaha, Hadi Hadi Kurniawan Henny Herwina Hirowati Ali Hirowati Ali, Hirowati Huda, Nadyatul Khaira Ida Ayu Putu Sri Widnyani Ilhamdi, Ilhamdi Indra Junaidi Zakaria Irawati Chaniago Irvan Fadli Wanda Jamsari Jamsari Janra, Muhammad Nazri Jon Affi, Jon Juliadmi, Dian Kevin Origia Kevin Origia Kharisma Putra Liza Aulia Yusfi M. Idris Mahmudul Hasan Mahmudul Hasan, Mahmudul Marlina Marlina Masayuki Sumida Masayuki Sumida, Masayuki Maulana, Imron Melinda, Annisa Menkher Manjas Meri Wulandari Muhamad Irsyad Muhammad Idris Muhammad Silmi Nelmi Fitria Netty Marusin Netty Marusin Nia Kurniawan Nia Kurniawan Nofrita Nofrita Nofrita Nofrita Nurhafitri, Amanda Nurul Aida Nurvalinda Nurvalinda Nuswantoro, Nuzul Ficky Nuzulia Irawati Oktaviana, Dili Padilla, Putri Rahma Prawira, Furqan Dwiki Lintang Putri, Emilya Rahmadina, Shovinda Ravelino Nesty Resti Rahayu Revis Asra Ridho Hendrikos Rifka Rahmat Riska Mayori Rita Permatasari Rivi Hamdani Rizaldi Rizaldi Rizia Irsa Salis, Viola Mutiara Saputri, Rahmi Agu Silvia Indra Sisca Dwi Yarni Sri Wahyuni Handayani Sri Wahyuni Handayani, Sri Wahyuni Suwirmen, Suwirmen Syaifullah Syaifullah Syaifullah Syaifullah Syaifullah Syamsuardi Syamsuardi Syandri, Hafrijal Takeshi Igawa Takeshi Igawa, Takeshi Tesri Maideliza Tofrizal Wilson Novarino Yarni, Sisca Dwi Yelvita Sari Yeni Gusma Yanti Yuliarni Syafrita Yurike Nova Edlin Zil Fadhillah Rahma Zozy Aneloi Noli Zulkarnain, Alivia