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All Journal HAYATI Journal of Biosciences Natural Science: Journal of Science and Technology Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pendidikan Matematika dan IPA AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Jurnal Biologi Universitas Andalas Indonesian Journal of Biotechnology JMPM (Jurnal Material dan Proses Manufaktur) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JURNAL KESEHATAN PERINTIS Majalah Kedokteran Andalas Menara Ilmu International Journal of Agricultural Sciences Jurnal Pembelajaran dan Biologi Nukleus Sainstek : Jurnal Sains dan Teknologi Best Journal (Biology Education, Sains and Technology) Jurnal Ners Journal of Ocean, Mechanical and Aerospace -science and engineering- (JOMAse) Makara Journal of Science
Djong Hon Tjong
Universitas Andalas
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Efek Ekstrak Etanol Rimpang Temu Mangga (Curcuma mangga Val.) Terhadap Sel β Pankreas Mencit Putih Yang Diinduksi Aloksan Secara Histologis Ridho Hendrikos; Netty Marusin; Djong Hon Tjong
Jurnal Biologi Universitas Andalas Vol 3, No 4 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.4.%p.2014

Abstract

The effect of mango ginger (Curcuma mangga Val.) ethanolic extract has been experimentally tested to β cell of alloxan-induced mice at the Laboratory Animal Development and Structure. The experiment aimed to identify the effectifity of the ethanolic extract to defend β cell from necrosis caused by the alloxan. The activity of the extract was evaluated by using control (A0 and A1) and treatments. All mice were induced by alloxan (150 mg/kg of body weight). One week later the extract was given orally to the mice at dose 200 (A2) 400 (A3) and 800 (A4) mg/kg of body weight (A4). The extract was given everyday for 21 days. Pancreas were isolated for each week. The observation of pancreas histology showed that extract 200 mg/kg body weight could decrease necrosis at Langerhans higher than the extract of 400 and 800 mg/kg of body weight. This results indicate that giving mango ginger extract at dose 200 mg/kg body weight could decrease β cell necrosis.Key words : mango ginger (Curcuma mangga Val.), β cell, alloxan, necrosis.
The Effect of Mesenchymal Stem Cell Wharton Jelly on Alzheimer’s Rat with Y-Maze Test Method Sisca Dwi Yarni; Hirowati Ali; Djong Hon Tjong
Majalah Kedokteran Andalas Vol 44, No 2 (2021): Online July 2021
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v44.i2.p112-116.2021

Abstract

Objective: This study aims to examine the y-maze test in alzheimer's rat after giving mesenchymal stem cell wharton jelly. Methods: This study used the y-maze test method to test the cognitive test of rat with the Y arm model. The treatment group consisted of a control, AlCl3, MSC-WJ and a combination of AlCl3 and MSC-WJ. The results obtained were analyzed in the form of a percentage. Results: The results of this study showed that the y-maze test showed that MSC-WJ had a significant effect compared to the AlCl3 control group in the alzheimer’s rat, namely 51.33% and 12.31%. Conclusion: In this study it was concluded that that MSC-WJ has a significant effect on alzheimer’s rat with y maze test.Keywords: AlCl3; Alzheimer’s; Mesenchymal Stem Cell Wharton Jelly
Change of Morphological Characters of an Introduced Population of Bilih Fish (Mystacoleucus padangensis Bleeker, Pisces: Cyprinidae) in Toba Lake Nofrita nofrita; Dahelmi dahelmi; Hafrijal Syandri; Djong Hon Tjong
International Journal of Agricultural Sciences Vol 3, No 1 (2019)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/ijasc.3.1.23-29.2019

Abstract

Population of Bilih Fish (Mystacoleucus padangensis) in Singkarak Lake has decreased as the effect of intensive exploitation and changes in water use function. Many appropriate works have been conducted for the fish conservation. One of them is introducing Bilih fish population to a new habitat,  Toba Lake. After nine years of releasing the fish, there was a hypothesis that these fish had undergone some changes on their morphology compared to their original population in Singkarak Lake. The changes could occure in some morphological characters and also the increase or decrease of their growth. This study was aimed at comparing morphology characters between Bilih fish introduced to Toba Lake with the original population in Singkarak Lake. Survey method was used and 400 samples of fish were taken from each Singkarak and Toba Lakes.  Parameters measured were 29 morphological characters. Data were analyzed using Mann Whitney U Test with SPSS 22. The results showed that 27 morphological characters of introduced fish had changed. Characters that had not changed were dorsal fin (anterior end)-anal fin distance (DFAF) and preanal distance (PD). Population of Bilih fish in Toba Lake might keep changing in the process of morphological character differentiation as long as environment factors still support the life of Bilih fish. 
Viral Load Terhadap Nilai Hematokrit Pada Infeksi Virus Dengue Rita Permatasari; Almurdi Almurdi; Djong Hon Tjong
JURNAL KESEHATAN PERINTIS Vol 7 No 1 (2020): JUNI 2020 : Jurnal Kesehatan Perintis (Perintis's Health Journal)
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.241 KB) | DOI: 10.33653/jkp.v7i1.413

Abstract

Demam berdarah adalah suatu penyakit infeksi yang disebabkan oleh virus dengue dan ditularkan melalui gigitan nyamuk Aedes aegypti. Virus dengue yang masuk ke tubuh manusia, akan menyerang sel dendritik dan sel retikuloendotelial lain seperti sel hati, makrofag sehingga menyebabkan diproduksinya mediator imun selular dan humoral. Mediator imun menyebabkan peningkatan permeabilitas kapiler sehingga cairan plasma keluar ke jaringan, akan menyebabkan hemokonsentrasi dan terjadinya peningkatan nilai hematokrit. Tujuan penelitian ini adalah untuk mengetahui hubungan viral load dengan nilai hematokrit pada infeksi virus dengue. Manfaat penelitian ini adalah memberikan penjelasan tentang mekanisme terjadinya infeksi virus dengue yang berpengaruh terhadap jumlah viral load dan nilai hematokrit pada infeksi virus dengue dan dapat digunakan sebagai referensi bagi pembaca maupun peneliti lainnya. Selain itu juga dapat dijadikan sebagai referensi diagnosis penyakit infeksi virus dengue serta meningkatkan kualitas penanganan terhadap penderita infeksi virus dengue. Metode Penelitian dengan desain deskriptif korelasional dengan 49 sampel yang dikumpulkan dari Laboratorium pasien infeksi dengue, data penelitian di analisis dengan software SPSS uji Pearson dengan menggunakan metode Nested-qPCR. Hasil Analisis penelitian menggunakan Software SPSS dengan uji korelasi Pearson, didapatkan hubungan jumlah viral load dengan nilai hematokrit pada infeksi dengue sangat lemah dengan nilai koefisien korelasi r = 0,163 dan nilai signifikan 0,381 > 0,05. Kesimpulan dari penelitian ini yaitu tidak terdapat hubungan bermakna antara Viral Load Dengan Nilai Hematokrit Pada Infeksi Virus Dengue.
Phylogenetic Study of Bufonidae (Amphibia: Anura) from Sumatera and Asia Based on Cytochrome B Gene Djong Hon Tjong; Dewi Imelda Roesma; Silvia Indra
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.15

Abstract

Bufonidae is widely distributed amphibian family around the world, including Indonesia (Sumatra) and Asia. Sumatra is an island which has separated because of sea level increase and create gene flows barrier for amphibian in Sumatra and Asia. The purpose of this research is to analyse Bufonidae phylogenetic which exist in several locations in Sumatra and Asia based on gene cytochrome b analysis. Samples were collected from six localities in West Sumatra. DNA extraction and amplification has been done in Genetics and Cell Biology Laboratory, Andalas University, whereas DNA sequencing were analyzed at MacroGen USA DNA Sequencing Laboratory, South Korea. About 14 tissue samples of Bufonidae from West Sumatra has been analyzed. The relationship of Bufonidae phylogenetics in Sumatra and Asia were divided into three main clusters. Duttaphrynus melanostictus in Sumatra is closer to Duttaphrynus melanostictus from India with 0,3-0,5% sequence divergence and it is a group of paraphyletic with Duttaphrynus melanostictus from Vietnam, Taiwan, and China.
Keanekaragaman Jenis Amfibi (Ordo: Anura) di Desa Pasia Laweh, Pesisir Selatan, Sumatra Barat Yeni Gusma Yanti; Muhammad Nazri Janra; Djong Hon Tjong
Al-Kauniyah: Jurnal Biologi Vol 15, No 2 (2022): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i2.19775

Abstract

AbstrakPenelitian mengenai keanekaragaman jenis amfibi (ordo: Anura) di Desa Pasia Laweh, Kabupaten Pesisir Selatan, Sumatra Barat ini bertujuan untuk mengetahui jenis-jenis amfibi beserta indeks keanekaragamannya pada beberapa habitat yang diteliti. Penelitian dilaksanakan dari bulan Juni sampai Desember 2019 menggunakan metode survei dengan penangkapan secara aktif (visual encounter) di empat tipe habitat yaitu hutan primer, sekunder, sungai, permukiman, dan sawah, dilanjutkan dengan pengukuran morfometrik dan pendeskripsian jenis yang didapat. Hasil penelitian ini didapati 23 jenis amfibi dari enam famili, yaitu Ranidae (7 jenis), Bufonidae (4 jenis), Dicroglossidae (5 jenis), Microhylidae (3 jenis), Megophryidae (1 jenis), dan Rhacophoridae (3 jenis). Jenis Pulchrana debussyi dan Ingerophrynus claviger yang sangat sedikit catatan distribusinya juga teramati pada penelitian ini. Jenis P. debussyi, Rhacophorus nigropalmatus, dan R. reinwardtii dikoleksi dari lapangan pada fase berudu dan berhasil diidentifikasi setelah dipelihara sampai menjadi katak muda (froglet); ciri-ciri morfologi berudu dan froglet digambarkan di dalam tulisan ini. Indeks keanekaragaman jenis dari habitat-habitat amfibi yang ada di Desa Pasia Laweh mengindikasikan bahwa kawasan ini tergolong berkeaneragaman amfibi sedang.Abstract The study on the diversity of amphibian (order Anura) in Pasia Laweh Village, Pesisir Selatan Regency, West Sumatra aimed to obtain species inventory for this amphibian order as well as to reveal the diversity index at its habitats. The study was conducted from June to December 2019, using survey method where samples collected through night visual encounter technique in four habitat types; primary and secondary forest, rivers, habitation and paddy fields. Collected specimens were further measured for their morphometric parameters and taxonomically described. The study recorded 23 amphibian species classified into six families; Ranidae (7 species), Bufonidae (4 species), Dicroglossidae (5 species), Microhylidae (3 species), Megophryidae (1 species) and Rhacophoridae (3 species). Species with distribution-lacking records, namely Pulchrana debussyi and Ingerophrynus claviger, were also observed in this study. Moreover, Pulchrana debussyi, Rhacophorus nigropalmatus and R. reinwardtii were collected as tadpoles, which then reared into identifiable froglets; hence, the description on tadpoles and froglets are provided in this paper for the respective species. Diversity index from studied habitats in Pasia Laweh Village indicated that the area had moderate amphibian diversity.
Nilai Leukosit, Eritrosit dan Trombosit pada Penderita Leukemia Limfoblastik Akut Pasien Anak Rifka Rahmat; Djong Hon Tjong; Almurdi Almurdi; Meri Wulandari
JURNAL KESEHATAN PERINTIS Vol 9 No 2 (2022): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v9i2.863

Abstract

Leukemia merupakan suatu penyakit keganasan sel darah yang ditandai proliferasi leukosit secara tidak terkendali dengan manifestasi adanya sel-sel abnormal. Di Sumatera Barat penyakit ini menunjukkan prevalensi leukemia sebesar 2,4%. Penelitian ini bertujuan untuk melihat gambaran nilai Leukosit, Eritrosit dan Trombosit pada Penderita Leukemia Limfoblastik Akut (LLA) Pasien Anak di RSUP DR. M. Djamil Padang. Populasi dalam penelitan ini adalah pasien LLA anak yang menjalani kontrol di poliklinik khusus anak dan melakukan pemeriksaan darah di laboratorium sentral RSUP Dr. M Djamil yang terdiri dari 12 anak laki-laki dan 6 orang anak perempuan. Sampel adalah pasien yang memenuhi kriteria inklusi. Parameter yang diperiksa adalah leukosit, eritrosit, dan trombosit. Prinsip pemeriksaan darah adalah flow cytometri dengan teknik impedance. Dari penelitian yang telah dilakukan bahwa hasil darah pasien LLA anak memperlihatkan rerata jumlah leukosit 72,10±155,91 pada laki-laki dan 4,50±3,01 pada perempuan, jumlah eritrosit 4,19±0,84 pada laki-laki dan 3,66±0,81 pada perempuan. Selanjutnya rerata trombosit 280±138,69 pada laki-laki dan 243±1,58 pada perempuan. Dari hasil tersebut bahwa terjadi peningkatan leukosit pada laki-laki (72,10±155,91) sedangkan pada anak perempuan masih dalam rentang normal (4,50±3,01) (standar normal leukosit: 4,5-13,5).
GENETIC VARIATIONS OF TWO Anguilla SPECIES IN THE WEST COAST OF WEST SUMATRA AND MENTAWAI USING RAPD Syaifullah syaifullah; Annisya Fitri; Djong Hon Tjong; Dewi Imelda Roesma; Indra Junaidi Zakaria
Jurnal Biologi Universitas Andalas Vol 11, No 1 (2023)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.11.1.39-45.2023

Abstract

Research on the genetic variation of Anguilla spp. in the waters of the West Coast of West Sumatra was carried out from February to June 2022. This study used the RAPD (random amplified polymorphic DNA) method. In this study, two Anguilla species were found, namely A. marmorata and A. bicolor bicolor. The highest heterozygosity value of A. marmorata in the Tarusan River was 0,2018, and A. bicolor bicolor in Mentawai was 0,2340. The values of gene flow (Nm) in A. marmorata and A. bicolor bicolor were 0.7592 and 1.6318. The values of genetic differentiation (GST) in A. marmorata and A. bicolor bicolor were 0.3971 and 0.2345, respectively. This shows that the value of genetic variation of A. marmorata and A. bicolor bicolor in several rivers on the west coast of West Sumatra is low.
Phylogenetic Analysis of Cyclocheilichthys apogon and Cyclocheilichthys armatus (Fish: Cyprinidae) from West Sumatra Dewi Imelda Roesma; Djong Hon Tjong; Syaifullah; Dyta Rabbani Aidil
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.895-906

Abstract

Cyclocheilichthys apogon (Beardless Barb fish) and Cyclocheilichthys armatus (Hihgfin Barb fish) in West Sumatra is known by the local name Turiak and Catua fish, respectively, are economically important species. Reducing populations and limited biological information about these fishes make their genetic diversity and phylogenetic relationships crucial. The genetic variation was investigated by analyzing the sequencing of a part of mitochondrial DNA Cytochrome b (Cyt b) and Cytochrome oxidase-I (COI) genes of 23 individuals collected from West Sumatra populations. Phylogenetic analysis revealed the monophyletic lineage of the Cyclocheilichthys group and supported Anematichthys as the synonym of Cyclocheilichthys. C. armatus was closely related to C. janthochir and separated from C. apogon with sequences divergence were 12.7-14.0% (Cyt b) and 9.4%-10.0% (COI). C. apogon and C. armatus have low haplotype diversity and genetic diversity, respectively. Genetic diversity within and among populations with sequences divergence was 0.0-0.8% (Cyt b) and 0.0%-0.7% (COI), respectively. The low-level diversity may signify a possibility of gene flow within and among populations. The current data suggests all populations as a single stock unit. Present genetic information is important to establishing effective population management, conservation, and breeding strategies for Turiak and Catua fish.
Deteksi crypstosporidium sp. pada pasien dengan kanker kolorektal Sri Wahyuni Handayani; Nuzulia Irawati; Djong Hon Tjong; Tofrizal Tofrizal; Elli Firdamila
Majalah Kedokteran Andalas Vol 46, No 7 (2023): Supplementary December 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i7.p%p.2023

Abstract

Cryptosporidium sp. adalah parasit obligat intraseluler yang menyerang sel epitel usus. Infeksinya mengakibatkan diare, malnutrisi, dehidrasi dan cedera usus terutama pada orang dengan gangguan imunitas. Tingginya infeksi Cryptosporidium pada pasien HIV-AIDS pada penelitian  sebelumnya menandakan sumber infeksi yang tinggi di lingkungan. Cryptosporidium dilaporkan memiliki hubungan dengan kanker kolorektal baru-baru ini. Tujuan: tujuan penelitian ini adalah untuk mengetahui prevalensi infeksi Cryptosporidium pada pasien dengan kanker kolorektal. Metode: metode yang digunakan observasional dengan desain cross-sectional study pada 43 pasien kanker kolorektal yang sesuai dengan kriteria yaitu pasien yang baru didiagnosis dan belum mendapatkan terapi onkologis. Pengumpulan sampel feses dilakukan dari bulan April hingga Agustus 2023. Pemeriksaan ookista dilakukan secara mikroskopik dengan pewarnaan tahan asam modifikasi ziehl neelsen. Hasil: hasil penelitian didapatkan 46,5% (20/43) pasien positif terinfeksi Cryptosporidium. Kesimpulan: kesimpulan hasil penelitian, infeksi Cryptosporidium tinggi pada pasien kanker kolorektal, terutama pada lokasi kanker rektum. Penelitian lebih lanjut untuk mengetahui spesies Cryptosporidium diperlukan untuk mengetahui  rute penularan dan sumber infeksi.
Co-Authors - Jumawita - Syaifullah . Mansyurdin Aadrean Aidil, Dyta Rabbani Aldino Fauzil Fanani Almurdi Almurdi Amanda Nurhafitri Amir Hamidy AMIR HAMIDY Anggraini, Debie Annisa Farma Annisa Khaira Annisya Fitri Anthoni Agustien Anthonie Agustien Anugrah Viona Agesi Arum Setiawan Asnul Fitria Asrori, Ikrima Aurora, Dhea Apriano Dahelmi Dahelmi Dahelmi David Gusman Dewi Imelda Roesma Dharmayanthi, Anik Budhi Dian Juliadmi Djoko T. Iskandar Djoko T. Iskandar, Djoko T. Dwitya Elvira Dyta Rabbani Aidil Elli Firdamila Endang Purwati RN Essy Harnelly fachrul reza, fachrul Fanny Adhy Putri Fanny Lestari Fauzan . Feskaharny Alamsjah Firdamila, Elli Fuji Astuti Febria G Gunawarman, G Gusman, David Hadi Addaha, Hadi Hadi Kurniawan Henny Herwina Hirowati Ali Hirowati Ali, Hirowati Huda, Nadyatul Khaira Ida Ayu Putu Sri Widnyani Ilhamdi, Ilhamdi Indra Junaidi Zakaria Irawati Chaniago Irvan Fadli Wanda Jamsari Jamsari Janra, Muhammad Nazri Jon Affi, Jon Juliadmi, Dian Kevin Origia Kevin Origia Kharisma Putra Liza Aulia Yusfi M. Idris Mahmudul Hasan Mahmudul Hasan, Mahmudul Marlina Marlina Masayuki Sumida Masayuki Sumida, Masayuki Maulana, Imron Melinda, Annisa Menkher Manjas Meri Wulandari Muhamad Irsyad Muhammad Idris Muhammad Silmi Nelmi Fitria Netty Marusin Netty Marusin Nia Kurniawan Nia Kurniawan Nofrita Nofrita Nofrita Nofrita Nurhafitri, Amanda Nurul Aida Nurvalinda Nurvalinda Nuswantoro, Nuzul Ficky Nuzulia Irawati Oktaviana, Dili Padilla, Putri Rahma Prawira, Furqan Dwiki Lintang Putri, Emilya Rahmadina, Shovinda Ravelino Nesty Resti Rahayu Revis Asra Ridho Hendrikos Rifka Rahmat Riska Mayori Rita Permatasari Rivi Hamdani Rizaldi Rizaldi Rizia Irsa Salis, Viola Mutiara Saputri, Rahmi Agu Silvia Indra Sisca Dwi Yarni Sri Wahyuni Handayani Sri Wahyuni Handayani, Sri Wahyuni Suwirmen, Suwirmen Syaifullah Syaifullah Syaifullah Syaifullah Syaifullah Syamsuardi Syamsuardi Syandri, Hafrijal Takeshi Igawa Takeshi Igawa, Takeshi Tesri Maideliza Tofrizal Wilson Novarino Yarni, Sisca Dwi Yelvita Sari Yeni Gusma Yanti Yuliarni Syafrita Yurike Nova Edlin Zil Fadhillah Rahma Zozy Aneloi Noli Zulkarnain, Alivia