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Journal : JOSAR (Journal of Students Academic Research)

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Sensitivity Test of Clove Leaf Essential Oil (Syzygium aromaticum) Against Heterotrophic Bacteria Isolated from Food Products Filayani, Muhammad Iqbal; Jana, Farida Nur; Fitriyah
JOSAR (Journal of Students Academic Research) Vol 10 No 2 (2025): September
Publisher : Universitas Islam Balitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35457/josar.v10i2.4940

Abstract

This study aimed to evaluate the antibacterial activity of clove leaf essential oil (Syzygium aromaticum) against heterotrophic bacteria isolated from food products. Clove leaves were collected from Malang Regency, Indonesia, and the essential oil was extracted via steam-water distillation. Bacterial isolates were obtained from food samples using serial dilution and pour plate techniques. Antibacterial activity was assessed using the agar diffusion method at three oil concentrations (50%, 75%, and 100%), with Amoxicillin trihydrate as the positive control and sterile distilled water as the negative control. The morphological characteristics of the isolates were documented, and inhibition zones were measured. Results indicated that inhibition strength increased with oil concentration up to 75%, with the largest numerical inhibition observed against isolate K2 at 100% concentration (31.3 mm), approaching the positive control’s efficacy. Statistical analyses confirmed that differences among treatments were significant (p < 0.001), except between 75% and 100% concentrations, suggesting a plateau in activity beyond the minimum inhibitory concentration. The findings highlight clove leaf essential oil’s potential as a natural antibacterial agent for food preservation, offering an environmentally sustainable alternative to synthetic antimicrobials. However, in vitro conditions may not fully represent performance in real food matrices, warranting further research on its application, stability, sensory effects, and synergistic combinations with other natural antimicrobials.
Co-Authors A'rof, Abas Bahar Agus Hermawan Agustin Putri Mardiawan Ah Syamli Ahmad Mahfudh Ahmad Mohammad Tidjani Aida Nur Azqiya Alda Ratna Sari Alhafiz, Muhammad Ali Mahsun Alvian, Dwiki Alvin Hadi Andriani Angraini Apri Kurniasih Ardiyanto, Dicky Aspri Asy'arie, Bima Fandi Baehaqi Bima Fandi Asy'arie Damanhuri Darmanto Darmanto Delma Khotimah Diah Komala Sari Diana Ni'matul Khusna Dimas Ilham Nur Rois Dimas Prasetya Dina Camelia ekawati Elly Purwanti Endang Masithoh Erna Ts Ernasari Ernasari Fatmawati, Kiki Febriani Indah Fajrotin Filayani, Muhammad Iqbal Firda Azzahra, Fadhila Fithrah Kamaliyah Friska, Yuliana Hardiyansyah Hasmi I Ketut Mahardika Ibnatur Husnul, Nisak Ruwah Imroatus Sholehah Indo Iya Mappaita Iri Hamzah Irmawati Isfaizah Ismiyanto JAMILAH Jana, Farida Nur Jelanti, Desi Jesika Selin Kamaliyah, Fithrah Komplit Raharjo Kriswanto Laila Nursafitri Lailatur Rahmah Leo Yosdimyati Romli Linda Suryani M. Yogi Riyantama Isjoni Mahdar Martaleni - Masruroh Maysaro Mei Sri Pratiwi Miftahur Ridho Mohammad Fauzi Muhammad Rosyid Muhammad Syihab As’ad Muhammad Tahir Muslimah, Izzatul Nancy Oktyajati Nisa, Hayatun Nourma Dewi Nur Azizah, Riska Nur Indah Sari Nur Marfu’ah, Istiyanti Oktaviano, Revandy Cekha Prastowo, Sri Handono Budi Priyanti, Ananda Dwi Qomariyah, Nurul Ulfa Rahmat Hidayat Raisa Aribatul Hamidah Raka Syukur Rakhmanita, Evrina Ramadhani, Erfan Ramiro, Jonathan Retno Herawati, Nunik Rifaldi Rina Dwi Rahayu Risthi, Alyssa Rita Zunarti Rizki Febri Eka Pradani Romlah Roni, Faishol Rosyid, Muhammad Rudi Haryadi, Rudi Rusmawati, Putry Septiani Selly Susanti Serli Silviyani, Yuli Anita sitikhomsiyati Sri Agustini Sri Agustini Sri Dewi Rahmawati Sri Widyantoro, Hanan Srie Juli Rachmawatie Stevani Susilia Sudarsono, Naswan Suharno Syska Purnama Sari, Syska Purnama Tasrif Shandy Taufik Aris Saputra Teuku Salfiyadi Titin Nur Jamila Trimayang Anisa Velia Septia Wulandari Vidya Urbaningrum Urbaningrum Wahyuningrum, Sri Rizqi Yelsi Putriana Lama Yuvita Oktarisa, Yuvita Z Slam