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All Journal Jurnal Kedokteran Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Kesehatan Prima The Indonesian Journal of Infectious Diseases Jurnal Pengabdian Magister Pendidikan IPA Indonesian Journal for Health Sciences (IJHS) Jurnal Ilmu Kefarmasian Indonesia Jurnal Gema Ngabdi Jurnal Abdi Insani Jurnal Kedokteran Universitas Palangka Raya Cermin Dunia Kedokteran JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jurnal Pepadu Jurnal Wicara Desa Jurnal Kesehatan Qamarul Huda
Eustachius Hagni Wardoyo
Faculty Of Medicine, Universitas Mataram
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Antibacterial Activity Test of Sumbawa White Honey against Escherichia coli ATCC 25922 Hulfifa, Lale Nandita; Rosyunita, Rosyunita; Wardoyo, Eustachius Hagni; Hasbi, Nurmi; Rahim, Adelia Riezka; Arnawati, Ida Ayu; Alamsyah, Abiel Dwi Cahya Firdaus; Vaidika, I Komang Satya; Hazaa, Saskia Safarina
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7407

Abstract

Due to their high mortality and morbidity rates, E. coli infections are a serious issue. Antimicrobial Resistance (AMR) is a result of this indiscriminate antibiotic use. One approach to address this issue is to utilize natural antimicrobials such as Sumbawa white honey. Sumbawa white honey contains antibacterial components like glucose and fructose (80%), water (17%), polyphenols, and hydrogen peroxide. This study aimed to analyze the antibacterial activity of white honey from Sumbawa against E. coli ATCC 25922. The research method employed was a laboratory experiment using a post test only control group design. Antibacterial testing was conducted using disc diffusion at concentrations of 25%, 75%, and 100%, with chloramphenicol as positive control and DMSO as negative control, and honey processing involved dilution. Antibacterial tests were repeated five times  and antibacterial activity was observed based on the diameter of inhibition zones around the disc. The outcomes showed that there was a restraint zone at 100 percent grouping of honey, to be specific 0.90 mm, while at 25% and 75% fixations there were no hindrance zones. According to the findings of this study, white honey from Sumbawa has a low antibacterial activity (0.90 mm), but this activity is ineffective against E. coli ATCC 25922. Further identification of the chemical components in Sumbawa white honey that are primarily responsible its antibacterial effect.
Profile and Antibiotic Assay of Cefotaxime-Resistant Lactic Acid Bacteria from The Caecum of Broiler Chickens Rosyunita, Rosyunita; Wardoyo, Eustachius Hagni; Rahim, Adelia Riezka; Nurmi , Hasbi
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.7850

Abstract

A health problem associated with increasing antibiotic resistance, the silent pandemic kills 700,000 people a year, mostly in Asia and Africa. In the case of probiotic or lactic acid bacteria, antibiotic resistance can enhance nutrient absorption in the host and prevent the colonization of pathogenic bacteria. To address this, the WHO has suggested using the One Health Tricycle strategy, which entails monitoring people, the environment, and animals. One facet of this investigation is animals, specifically chickens. This study aims to identify the antibiotic resistance and profile of cefotaxime-resistant lactic acid bacteria (LAB) from broiler chicken cecum. Isolating LAB from the chicken's cecum is one of the techniques employed. After calculating the prevalence of resistant bacteria, the bacteria were characterized as macroscopic, microscopic, and biochemically. Testing the antibiotics against LAB was the next stage. According to the study's findings, the bacterial colonies were tiny to medium-sized, white, spherical, and convex, with complete margins. Gram-positive bacilli were identified by Gram staining. The findings of the biochemical tests were negative for oxidative and catalase, positive for glucose, and negative for other biochemical tests. According to antibiotic sensitivity testing, LAB was 90.9% resistant to Vancomycin, 63.63% to Chloramphenicol, and 100% to Erythromycin, Aztreonam, and Ceftriaxone. According to these findings, LAB in the chicken caecum has become resistant to several antibiotics. To give a better picture of the balance between pathogenic and helpful bacteria in the chicken cecum, more LAB testing against pathogenic bacteria is required.
Identification of Antibiotic-Resistant Gram Positive Bacteria from Broiler Caecum in The Slaughterhouse of Mataram City Rizqullah, Rifqi; Wardoyo, Eustachius Hagni; Rahim, Adelia Riezka; Rosyunita, Rosyunita; Hasbi, Nurmi; Indratama, I Nyoman Yudayana
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8466

Abstract

The subtherapeutic use of antibiotics as Antimicrobial Growth Promoters (AGPs) in broilers has accelerated Antimicrobial Drug Resistance (AMR) in gut microbiota, posing a global threat. This study aimed to analyze the population, morphology, catalase test results, and antibiotic sensitivity of erythromycin and vancomycin to cefotaxime-resistant Gram-positive bacteria in the caecum of broilers from Mataram City slaughterhouses. Using exploratory descriptive method, five caecum samples were analyzed by Total Plate Count (TPC) on Man Rogosa Sharpe Agar (MRSA) media with and without cefotaxime, and incubated on Mannitol Salt Agar (MSA). Results revealed uniform bacterial morphology on MRSA (small, round, convex, entire edge, white, Gram-positive colonies) but varied morphologies on MSA. Catalase tests were negative on MRSA but mixed on MSA. Resistance to erythromycin and vancomycin was 80% on MRSA, while on MSA, erythromycin resistance reached 62.5% with variable vancomycin inhibition zones. The prevalence of cefotaxime-resistant bacteria was 5.24%. This study highlights diverse morphological, catalase, and antibiotic sensitivity profiles in cefotaxime-resistant bacteria, particularly on MSA. These findings underscore the need for stricter antibiotic use regulations and further research to mitigate AMR spread in poultry production.
Tropical Fruit Peel Extracts and Their Potential in Antibacterial Product Formulation: A Literature Review Hasbi, Nurmi; Rosyunita, Rosyunita; Hanifa, Nisa Isnaeni; Wardoyo, Eustachius Hagni; Rahim, Adelya Riezka
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10360

Abstract

Bacterial infections caused by pathogenic microorganisms remain a serious global health issue, exacerbated by the increasing incidence of antimicrobial resistance. Although synthetic antibacterial agents are effective, concerns have arisen regarding their long-term toxicity, bioaccumulation, and environmental impact. Consequently, the exploration of natural, safe, and eco-friendly antibacterial sources has gained significant attention. Tropical fruit peels, which are abundant agro-industrial by-products, contain diverse bioactive compounds such as flavonoids, tannins, saponins, and alkaloids that exhibit strong antibacterial potential. This literature review systematically evaluates the potential of tropical fruit peel extracts as active ingredients in antibacterial product formulations. Using a systematic review method, articles were collected from databases such as PubMed and Google Scholar, focusing on studies published between 2015–2025 that assessed the antibacterial activity of ethanol extracts from tropical fruit peels against bacteria including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Klebsiella pneumoniae. The findings indicate that peels of pineapple (Ananas comosus), banana (Musa spp.), mango (Mangifera indica), papaya (Carica papaya), pomelo (Citrus maxima), mangosteen (Garcinia mangostana), and durian (Durio zibethinus) show significant antibacterial activity. These extracts have been successfully formulated into antiseptic soaps, hand sanitizers, nanoemulgels, and herbal mouthwashes, offering effective and sustainable alternatives to synthetic antibacterial agents.
SIMULASI IMPLEMENTASI KESIAPSIAGAAN MODEL EMERGING-REEMERGING INFECTIOUS DISEASES (EREIDS) DI PELABUHAN LEMBAR Wardoyo, Eustachius Hagni; Widiastuti, Ida Ayu Eka; Yudhanto, Didit; Susani, Yoga Pamungkas; Affarah, Wahyu Sulistya; Dirja, Bayu Tirta; Sari, Putu Suwita; Nuriastuti, Novera; GD, Ni Made Saithanya; Putri, Dyah Ayu; P, Novanda Dila
Jurnal Pepadu Vol 5 No 4 (2024): Jurnal PEPADU
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/pepadu.v5i4.5882

Abstract

Antisipasi dalam menghadapi ancaman EREIDs perlu dilakukan sebagai upaya pengurangan dampak buruk bagi penduduk. Sebagai tempat berkumpulnya orang banyak dalam jangka waktu lama, kapal penumpang merupakan salah satu tempat berisiko menjadi hotspot penularan EREIDs yang efektif. Simulasi kesiapsiagaan EREIDs bertujuan untuk penyajian model EREIDs dipelabuhan dan diskusi perbaikan model EREIDs. Pelaksanaan simulasi dilakukan secara hybrid mengundang Pelindo, KSOP, BKK dan operator kapal dengan materi: kesan data kesiapsiagaan EREIDs di Lembar, diskusi tupoksi masing-masing stakeholder dan menggali saran perbaikan kedepannya. Penyajian model EREIDs meliputi: 1) pengendalian teknis (1.1. fasilitas sarpras, 1.2. modifikasi sarpras dan 1.3 penyediaan instrument keselamatan), 2) pengendalian administratif (2.1. penyediaan SOP keselamatan, 2.2 Pelaksana SOP Keselamatan dan 2.3 Pengawasan pelaksanaan SOP keselamatan) dan 3) penggunaan alat pelindung diri (3.1. penyedia APD dan 3.2. Edukasi penggunaan APD). Secara kualitatif didapatkan bahwa peran utama stakeholder masing-masing: 1.1. Pelindo; 1.2. BKK; 1.3. Pelindo; 2.1. BKK; 2.2. Pelindo, Operator Kapal, KSOP; 2.3. BKK, KSOP; 3.1. Pelindo, BKK; 3.2. BKK. Adapun tantangan kesiapsiagaan EREIDs di Pelabuhan dan Kapal adalah koordinasi antar stakeholder, permintaan catatan kejadian, dan rekapitulasi insiden yang masih harus diselaraskan kembali. Dapat disimpulkan bahwa peningkatan kesiapsiagaan EREIDs dipelabuhan dan kapal merupakan penyiapan lintas sector dan lintas bidang intra dan inter stakeholder. Semua stakeholder memiliki peran krusial dalam mencegah masuknya wabah dari luar pulau dan mengirim wabah keluar pulau.
PELATIHAN BAHASA INGGRIS BAGI REMAJA SMP/SMA DESA LOYOK OLEH KKN-PMD UNIVERSITAS MATARAM 2023 Solihah, Maulida; Hidayat, Rizky; Jannah, Miftahul; Febriana, Rista; Sofiatuddin, Baiq; Gunawan, M. Haerul; Suazhari, Muhammad Adam; Andini, Ayu Fitri; Aimar, Lalu Muhammad Fascal; Haerunnisa, Baiq Vidia; Wardoyo, Eustachius Hagni
Jurnal Wicara Vol 1 No 6 (2023): Jurnal Wicara Desa
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/wicara.v1i6.3463

Abstract

Loyok Village is one of the Tourism Villages in Sikur District, East Lombok Regency. Many tourists come both from within and outside the country to see and enjoy the tourist objects in Loyok Village such as the woven bamboo art shop and the beautiful views of the rice fields. The English language training activities conducted by the University of Mataram KKN-PMD 2023 aim to improve English language skills for junior high/high school youth in Loyok Village. It is hoped that this capacity building will benefit the people of Loyok Village to make it easier for them to communicate with foreign tourists who come to Loyok Village. The training was attended by 23 representative participants from each hamlet in Loyok Village. This training includes several Speaking materials such as Greeting, Asking and Giving Opinion, Agree and Disagree, and Asking and Giving Direction. This training activity is carried out for 3 weeks, namely every Wednesday, Thursday and Friday. This English training activity brings directly tutors who are experienced in speaking English.
The Effect of Roasting Methods of Ingredients The Songgak Tribe Sasak Herbal on Active Compounds Using Gas Chromatography Mas Spectroscopy (Gc-Ms) Analysis) Agrijanti, Agrijanti; Kusuma Dewi, Lale Budi; Khusuma, Ari; Wardoyo, Eustachius Hagni
Jurnal Kesehatan Prima Vol. 18 No. 2 (2024): AUGUST
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkp.v18i2.1540

Abstract

Songgak is a concoction of the Sasak tribe of Lombok in West Nusa Tenggara. It consists of coffee mixed with groundwood, cloves, black pepper, coriander, and nutmeg. The original recipe involves roasting the spices before mixing them with coffee, namely using a frying pan over a stove. The roasting process is carried out without controlling the temperature and roasting time, so the roasted ingredients need to be standardized(Dewi et  al., 2021). GCMS is a tool used to analyze compounds in samples using chemical separation methods, mainly chromatography. The compounds in the mixture are separated in a chromatographic column. The separation mechanism between several compounds occurs due to differences in the solubility values of each in the moving solvent and differences in the absorption of each of the competing compounds into the stationary phase (Gross, 2017). In this research, variations will be carried out on spices, namely T1 original treatment of roasting spices over fire for 8 minutes, T2 drying spices in sunlight, T3 oven for 5 minutes, T5 oven for 5 minutes, T4 oven for 15 minutes and T5 oven for 15 minutes. In the T4 treatment, namely oven for 10 minutes, the results of the analysis of the substance content with the most types, namely 20 types, were obtained. The active anti-microbial compound found in all treatments was Hexadecanoic acid, 2-hydroxy-1-(h)(C19 H38 O4) BM:330, which helps inhibit the growth of cancer cells. (National Library Medicine, n.d.). Octadecanoic acid (CAS) Stear (C18 H34 O2) BM:282 Anti-inflammatory, Antiandrogenic Cancer prevention, Hypocholesterolemia Dermatigenic, 5-Alpha reductase inhibitor, Anemiagenic, Insecticide (Natarajan et al., 2019) and Tetradecanoic acid (CAS) Myris (C14 H28 O2) BM:228 as anti-inflammatory and anti-nociceptive. 
The Association between Daily Contact, Working Period and Biosecurity to Colonization of Extended-Spectrum Beta Lactamase-Producing Escherichia coli of Poultry Workers Wardoyo, Eustachius Hagni; John, Sonya Ananda Elya; Ali, Muhamad
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.8076

Abstract

Extended-spectrum beta lactamase producing Escherichia coli (ESBL-Ec) colonization in poultry worker is one prominent of zoonotic problem, in which of it, daily contact, working period and biosecurity play role. This study aimed to identify association between daily contact, working period and biosecurity to ESBL-Ec’ colonization. Rectal swab was applied to poultry workers to identify phenotypic ESBL-Ec. Duration of contact, working period and biosecurity parameter data was obtained through structured questionnaire. A number of 51 poultry workers with a mean age of 42.7 years, and 54.9% are male. Nineteen poultry workers (37.3%) was tested positive for ESBL-Ec. Daily contact of ≥ 2 hours is not significant (p = 0.291; 95% CI) while working period of ≥ 3 years is significant (p = 0.024; 95% CI) to ESBL-Ec’ colonization. Twenty-one biosecurity parameters were measured, with 3 parameters significant association: sick chicken isolation (p = 0.022), existence of quarantine shed (p = 0.018) and past biosecurity training (p = 0.047). In conclusion, working period ≥ 3 years, sick chicken isolation, existence of quarantine shed and past biosecurity training is associated with ESBL-Ec’ colonization to farmer.
Antibacterial Activity of Sumbawa Forest Honey (Apis dorsata) against Klebsiella pneumoniae ATCC BA1705 Untari, Lania Pradiva; Wardoyo, Eustachius Hagni; Dirja, Bayu Tirta
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10159

Abstract

Klebsiella pneumoniae is an opportunistic bacterium often associated with antibiotic resistance, necessitating the investigation of natural antibacterial alternatives. Honey is recognized for its bioactive ingredients that possess antibacterial properties. This research sought to assess the antibacterial efficacy of Sumbawa forest honey (Apis dorsata) against K. pneumoniae ATCC BA1705 by inhibition zone test. A laboratory experiment was performed using the well diffusion technique. K. pneumoniae suspensions (McFarland 0.5) were injected onto Mueller Hinton Agar. Wells (Ø 6 mm) were filled with 100 µL of honey at concentrations of 25%, 50%, 75%, and 100%. Tetracycline (1 µg/µL) acted as the positive control, while distilled water functioned as the negative control. Plates were incubated at 35±2°C for a duration of 18 to 24 hours. Inhibition zones were quantified using a caliper, and each experiment was conducted in sextuplicate. Data were expressed as mean ± standard deviation. No inhibitory zones were found at 25% and 50% concentrations (0 mm). Antibacterial activity was seen at 75% concentration, yielding a mean diameter of 10.33±SD mm, and raised to 100% concentration, resulting in a mean diameter of 11.33±SD mm. Tetracycline generated a 26 mm inhibitory zone, while distilled water exhibited none (0 mm). The conclusion is Sumbawa forest honey exhibits concentration-dependent antibacterial activity against K. pneumoniae, with moderate inhibition at concentrations ≥75%. Although less effective than tetracycline, these findings support the potential role of honey as a complementary antibacterial agent.
Co-Authors Adnanto Wiweko Agrijanti, Agrijanti Ahmad Taufik S Aimar, Lalu Muhammad Fascal Ajeng Retno Akbar Gazali, Akbar Alamsyah, Abiel Dwi Cahya Firdaus Andini, Ayu Fitri Ardiana Ekawanti Arfi Syamsun Arnawati, Ida Ayu Bayu Tirta Dirja Bayu Tirta Dirja Candra Eka Puspitasari Catarina Budyono, Catarina Citami, Niko Claresta Salsabila Decky Aditya Zulkarnaen Dewi Suryani Dewi Suryani Dewi, Lale Budi Kusuma Didit Yudhanto Dini Suryani Dirja, Bayu Tirta Dwiana Ocvyanty Edi Prasetyo Wibowo Emira Alifia Enty Tjoa Enty, Enty Eva Triani Febriana, Rista Febriza Risti Widyastuthi GD, Ni Made Saithanya Gunawan, M. Haerul H Moehario, Lucky Haerunnisa, Baiq Vidia Hanifa, Nisa Isnaeni Hasbi, Nurmi - Haza, Saskia Safarina Hazaa, Saskia Safarina Hulfifa, Lale Nandhita Hulfifa, Lale Nandita I Gede Jayantika I Gede Yasa Asmara I Gst Alit Rai Sudiadnya Ida Ayu Eka Widiastuti Ida Bagus Alit Ika Primayanti Indratama, I Nyoman Yudayana John, Sonya Ananda Elya Khusuma, Ari Linda Silvana Sari Lucky H Moehario Miftahul Jannah Monalisa Nasrul Muhamad Ali Muhammad Ghalvan Sahidu Muhammad Luthfi Mulya, Shania Hafitsa Muthia Cenderadewi, Muthia Ni Made Amelia Ratnata Dewi Nuriastuti, Novera Nurmi , Hasbi Ocvyanty, Dwiana P, Novanda Dila Prima Belia Fathana Putri, Dyah Ayu Qurrata'yuni Pratiwi Rahim, Adelia Riezka Rahim, Adelya Riezka Raisya Hasina Ramses Indriawan Rizky Hidayat, Rizky Rizqullah, Rifqi Rolly Armand Rosyunita Rosyunita, Rosyunita Saputra, Nurman Sari, Putu Suwita Shania Hafitsa Mulya Sofiatuddin, Baiq Solihah, Maulida Srikus Saptaningtyas, Rini Suazhari, Muhammad Adam Teguh Sarry Hartono, Teguh Sarry Titi Pambudi Karuniawaty Triana Dyah Cahyawati Untari, Lania Pradiva Vaidika, I Komang Satya Validika, I Komang Satya Wahyu Sulistya Affarah Wahyu Sulistya Affarah, Wahyu Sulistya Yoga Pamungkas Susani Yohanes Juliantoni Yunita Sabrina Yunita Sabrina, Yunita