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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Dentika Dental Journal MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Majalah Obat Tradisional Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry JIKG (Jurnal Ilmu Kedokteran Gigi) Journal of Indonesian Dental Association B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah DedikasiMU: Journal of Community Service Jurnal Abdi Masyarakat Indonesia JURNAL PENGABDIAN PENDIDIKAN MASYARAKAT (JPPM) Odonto dental journal

Juni Handajani

Universitas Gadjah Mada

Author-ID : 273943

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Efek Pengunyahan Permen Karet Gula dan Xylitol terhadap Pertumbuhan Bakteri Streptococcus Mutans pada Plak Gigi Volanda Kusumaningsari; Juni Handajani
Majalah Kedokteran Gigi Indonesia Vol 18, No 1 (2011): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Latar Belakang. Manusia memiliki flora normal yang hidup di dalam tubuhnya. Salah satu flora normal di rongga mulut adalah bakteri Streptococcus mutans yang memiliki kaitan erat dengan insidensi karies. Sekarang banyak beredar produk permen karet di masyarakat yang mengandung gula dan xylitol. Tujuan. Penelitian ini bertujuan untuk mengetahui efek mengunyah permen karet gula dan xylitol terhadap pertumbuhan bakteri S. mutans pada plak gigi. Metode penelitian. Subjek penelitian berjumlah 15 orang dibagi menjadi 3 kelompok masing-masing 5 orang, yaitu mengunyah permen karet gula, xylitol dan buah ape I sebagai kontrol. Pengambilan plak dilakukan pada hari pertama sebelum diberi perlakuan (pre-tesQ dan hari keempat setelah diberi perlakuan (post-tesQ. Subjek diminta untuk melakukan scalling sebelum pengambilan data pre-test agar skor plak awal semua subjek nol. Subjek mengunyah 1 butir permen karet setelah makan atau 3 kali sehari selama 10 menit. Subjek tidak diperbolehkan makan dan minum selama 1 jam sebelum pengambilan data post-test. Plak ditanam dalam media agar TYCSB (tryptone-yeast-cysteine-sucrose-bacitracin) lalu diinkubasi selama 72 jam pada kondisi anaerob dengan suhu 37°C. Penghitungan jumlah bakteri S. mutans dilakukan secara manual menggunakan counter. Analisis data dengan uji statistik Mann-Whitney. Hasil penelitian menunjukkan adanya peningkatan jumlah bakteri S. mutans pada plak gigi pada pengunyahan permen karet kandungan gula, sedangkan pengunyahan permen karet kandungan xylitol dapat menurunkan bermakna jumlah bakteri S. mutans. Kesimpulan. Pengunyahan perm en karet xylitol dapat menurunkan jumlah S. mutans pada plak gigi tetapi permen karet gula meningkatkan jumlah S. mutans.

The effect of in vitro royal jelly provision on adhesion of Pseudomonas aeruginosa Sifra Kristina Hartono; Tetiana Haniastuti; Heni Susilowati; Juni Handajani; Alma Linggar Jonarta
Majalah Kedokteran Gigi Indonesia Vol 5, No 1 (2019): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic bacterium, which could aggressively infect immunocompromised patients and thus, cause high mortality rate. In addition, P. aeruginosa in oropharynx could be aspirated and cause ventilator associated pneumonia. Royal jelly is one of bee’s products that has been used for therapeutic needs including antibacteria. Adherence factor of P. aeruginosa were flagelum, pili and lectin. The aim of the study was to determine the effect of royal jelly to P. aeruginosa adhesion. Suspension of P. aeruginosa (ATCC® 27853) was incubated at 37 °C for 18 h. Treatment groups were exposed to royal jelly with several concentrations, 2%, 4%, 6%; while distilled water was being used as negative control. Bacterial adhesion test was determined using spectrophotometer λ = 600 nm to measure optical density values of adhered bacterial suspension in tubes. The result of one-way ANOVA showed significant differences (p<0.05) of optical density values among groups indicating that royal jelly affected the bacterial adhesion. LSD results showed significant difference of optical density values between 2%, 4%, and 6% royal jelly compared to distilled water. Six percent of royal jelly had the least optical density value compared to the other groups. In conclusion, royal jelly has the ability to decrease adhesion of P. aeruginosa. Six percent of royal jelly has better ability to decrease adhesion of P. aeruginosa than other concentrations.

Effect of salat (prayer) activity on salivary status and cortisol level Nadia Rully Auliawati; Layung Sekar Prabarayi; Rais Aliffandy Damroni; Juni Handajani
Majalah Kedokteran Gigi Indonesia Vol 5, No 2 (2019): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.36959

The majority of Indonesian people are Muslim who perform the obligatory prayers five times each day known as salat. The series of activities in salat begins with wudu with a gargle movement that increases salivary secretion through mechanical stimulation. The movement of ruku and sujood when salat causes oxygen-rich blood to flow to the brain is well suited. When a person feels calmer and more psychically relaxed after performing salat and wudu, the parasympathetic nerves increase so normal cortisol levels can secrete more saliva which is aqueous with normal acidity (pH). Oral health will affect the overall health of the body. This study aimed to determine the effect of salat and wudu on the salivary flow rate, pH, and cortisol level as an indicator of oral health. The design of this study was the static group pretest and posttest design. The study was conducted by comparing the value of salivary flow rate, pH, and cortisol levels in 20 subjects consisting of 10 subjects as the treatment group and 10 subjects as the control. Saliva was collected without stimulation before and after salat activity for five minutes, followed by measurement of salivary flow rate and pH level. Salivary cortisol level was measured using Cortisol Assay Elisa kit with λ 540 nm. The results showed an increase in salivary pH, flow rate and cortisol level after the salat activity but there was no significant difference by Kruskal-Wallis test but there was a strong correlation using Kendall’s tau_b test. In conclusion, salat activity may have correlation with an increase in salivary pH, flow rate and cortisol level.

Neutrophil count in the gingival wound healing process after apitoxin gel application (gingival wound healing model on Wistar rats) Urfa Tabtila; Swastiana Eka Yunita; Muhammad Nabil Pratama; Juni Handajani
Majalah Kedokteran Gigi Indonesia Vol 6, No 2 (2020): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.37084

Periodontal disease has a high prevalence in Indonesia. Gingival disease is a periodontal disease if it is severe, requires surgical therapy called a gingivectomy. Surgical periodontal procedures lead to a gingival wound that is usually treatedusing a periodontal pack. Periodontal pack has no curative property in general but assists in a wound healing process by protecting tissues. Neutrophils are inflammatory cells that act as a host’s first defense against infectious pathogens and they are important in wound healing. Wound healing can be accelerated by apitoxin gel from honeybee Apis mellifera species. The aim of this study was to determine the effectiveness of apitoxin gel on neutrophil count on gingival woundhealing process. Medical bee venom powder (Umea, Sweden) was mixed with double-distilled water to prepare a concentration of 0.01% bee venom. The solution was mixed with 10% propylene glycol, 0.01% methylparaben, and3% CMC-sodium. Thirty six gingiva of 3 month-old male Wistar rats (weighing 200-250 gram) were injured using punch biopsy (3 mm in diameter). They were treated in 4 groups: apitoxin gel, apitoxin gel with a periodontal pack, NSAIDsFlamar® gel with periodontal pack, and a periodontal pack. Three Wistar rats from each group were euthanized and their jaws were taken after 48 hours treatment to make histology slides with Hematoxylin Eosin (HE) staining. Neutrophil counts were observed. The data were analyzed using Anova test with 95% confidence level. The results showed there were significan defferebces among 4 groups (p<0.05). The highest neutrophil count on the 2nd day was found in the apitoxin gel induced the 2 day of gingival wound healing process.

EFEK BERKUMUR EKSTRAK PEGAGAN (Centella asiatica (L.) Urban) KONSENTRASI 40% DAN 50% TERHADAP AKTIVITAS SPESIFIK GLUTATHIONE S-TRANSFERASE PADA SALIVA PENDERITA GINGIVITIS SEDANG Dhika Paramita Prameswari; Juni Handajani
Majalah Obat Tradisional Vol 15, No 3 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Glutathione S-transferase (GST) is enzyme in saliva that catalyzes the nucleophilic conjugation reaction of glutathione (GSH) with many diverse electrophilic substrates and has major mechanism in detoxification process of xenobiotics. Pegagan (Centella asiatica (L.) Urban) is contain flavonoid as a xenobiotic component that have an ability to inhibit the specific activity of GST. This study was aiming to understand the effect of gargling with centella’s extract concentration 40% and 50% to inhibit the specific activity of GST in saliva of gingivitis patient. Under ethical clearance from local Ethics Committee from Medical Faculty, Gadjah Mada University, 30 patients of moderate ingivitis divided into two groups: 20 person as treated group were instructed to gargle centella’s extracts concentration 50% and 60%, and 10 person as positive control group was given Hexetidine 0.1%. Every subject was gargling as much as 5 ml every morning (after woke up) and every night (before sleeping) for 5 days constitutively. Saliva was collected before and after treatment. GST’s specific activity was measured using spectrophotometer at 340 nm wavelength and gingivitis status was using Gingival Index (GI). This study showed that using centella’s extract concentration 50% could down regulate the GST’s specific activity saliva and GI score. In conclusion, centella’s extract concentration 50% was indicating better compare with concentration 40% to accelerate of gingivitis healing.

EFEK ANTIINFLAMASI EKSTRAK DAUN SEMBUKAN (Paederia scandens) PADA TIKUS WISTAR Evy Tri Utami; Rebecca Azary Kuncoro; Islamy Rahma Hutami; Finsa Tisna Sari; Juni Handajani
Majalah Obat Tradisional Vol 16, No 2 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Inflammation is a protective action that role on defending mechanism to defeat agent causing cell injury. Glucoside of skunkvine (Paederia scandens) has anti-inflammation effect. The aim of this study was to know the anti anti-inflammatory effect of skunkvine (Paederia scandens) extract on artificial edema in Wistar rat. Thirty-five Wistar rats were divided into 7 groups, each group consisted 5 rats. In the control and treatment groups, aquadest (negative control), fenilbutazon (positive control), skunkvine extracts in dose 10mg/kgBW, 20mg/kgBW 30mg/kgBW, 40mg/kgBW, and 50mg/kgBW were administered per orally. In order to induce the edema, one hour after administration, in each group, right hind paw of the rats was injected subcutaneously with by 1% carrageenin. The volume measurement performed with one hour interval for 5 hours assessing with pletysmometer. The data were analyzed by ANOVA. The result of this study showed there was significant difference on group with skunkvine extract 20mg/kg BW to negative control and positive control. In conclusion, that the skunkvine extract 20 mg/kgBW has potency as anti-inflammation substance.

Perbandingan sitotoksisitas tiga jenis algyrogel terhadap sel fibroblas (Citotoxicity comparation of three types of algyrogel on fibroblast cells) Stefany Elan Saktiyawardani; Hardono Jaya Lauson; Anugerah Pekerti Astamurtiningrum; Mahadna Aulia Rahmah; Pramana Pananja Putra; Juni Handajani
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 3 (2014): September 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: The prevalence of osteoporosis and bone defects in the world and Indonesia increase each year. Algyrogel is a alginate-based hydrogel material recently very popular used as a bone substitute through injectable bone substitute (IBs) method. One of advantages algyrogel is biocompatibility to the body tissues. some natural-based polymer (alginate, chitosan, dan hyaluronat) have different biocompatibility. Purpose: The study was aimed to determine the cytotoxicity of three types algyrogel against fibroblast cells. Methods: This study used three types algyrogel, there were algyrogel containing alginate (Alg), a mixture of alginate with chitosan (Alg/Ch), and a mixture of alginate with hyaluronic acid (Alg/Ha). Each type algyrogel was exposed in the Vero cell line of fibroblast with volume 2 μL; 4 μL; 6 μL; 8 μL; 10 μL; 12 μL; 14 μL; 16 μL; 18 μL; and 20 μL respectively. The cytotoxicity performed by MTT method to determine the percentage of cell death. The optical density was measured by an ELIsA plate reader then data was analyzed using ANOVA and probit. Results: The percentage of cell death in all groups of algyrogel have below 30%. LC50 value at Alg was 651.017 mg/mL (21.56%), Alg/Ch was 280.478 mg/mL (20.91%), and Alg/Ha was 1054.094 mg/ mL (17.2%). Conclusion: Alg/Ha has the lowest cytotoxicity on fibroblast cells and may have potential as an osteoconductor-synthetic bone product.Latar belakang: Prevalensi osteoporosis dan cacat tulang di Indonesia maupun dunia semakin meningkat tiap tahunnya. Algyrogel sebagai bahan hidrogel yang berbasis alginat akhir-akhir ini sangat popular digunakan sebagai bahan pengganti tulang melalui metode Injectable Bone substitute. Kelebihan algyrogel antara lain biokompatibel terhadap jaringan tubuh. Beberapa hidrogel berbasis polimer alami (alginat, chitosan, dan hyaluronat) memiliki perbedaan biokompatibilitas. Tujuan: studi ini bertujuan meneliti sitotoksisitas tiga jenis algyrogel terhadap sel fibroblas. Metode: Penelitian ini menggunakan 3 jenis algyrogel yaitu algyrogel dengan kandungan alginat (Alg), campuran alginat dengan chitosan (Alg/Ch), dan campuran alginat dengan asam hyaluronat (Alg/Ha). setiap jenis algyrogel dipaparkan pada sel fibroblas jenis Vero cell line dengan volume 2 μL; 4 μL; 6 μL; 8 μL; 10 μL; 12 μL; 14 μL; 16 μL; 18 μL; dan 20 μL. Pengujian sitotoksisitas dilakukan dengan metode MTT untuk mengetahui persentase kematian sel. Penghitungan optical density menggunakan ELIsA plate reader lalu data diuji Anova dan probit. Hasil: Persentase kematian sel pada semua jenis algyrogel memiliki angka dibawah 30%. Nilai LC50 pada Alg sebesar 651,017 μg/μL (21,56%), Alg/Ch sebesar 280,478 μg/μL (20.91%), dan "> Alg/Ha sebesar 1054,094 μg/μL (17,2%). Simpulan: Alg/Ha memiliki sitotoksisitas paling rendah pada sel fibroblas sehingga memiliki potensi sebagai produk osteokonduktor sintesis tulang.

Respon inflamasi pulpa gigi tikus Sprague Dawley setelah aplikasi bahan etsa ethylene diamine tetraacetic acid 19% dan asam fosfat 37% Nadie Fatimatuzzahro; Tetiana Haniastuti; Juni Handajani
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 4 (2013): December 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Etching agents such as ethylene diamine tetraacetic acid (EDTA) and phosphoric acid which are widely used in adhesive restoration system, are aimed to increase retention of restorative materials; however, these agents may induce inflammation of dental pulp. The major function of the inflammatory response is to remove invading pathogens or damaged tissue/ cells and therefore, initiate repair. Neutrophils and macrophages are motile phagocytes that constitute the body's first line of defense. Purpose: The purpose of the present research was to study the effect of 19% EDTA and 37% phosphoric acid for etching application agents on the inflammatory response of the dental pulp. Methods: Forty-five male Sprague Dawley rats were divided into 3 groups. Cavity preparation was made on the occlusal surface of maxillary first molar using a round diamond bur. Nineteen percent of EDTA, 37% phosphoric acid, and distilled water were applied on the surface of the cavity of the teeth in group I, II and III respectively. The rats were sacrified at 1, 3, 5, 7, and 14 days after the application (n=3 for each day). The specimens were then processed histologically and stained with hematoxylin eosin. Results: ANOVA showed a significant difference (p<0.05) among treatment groups, indicating that etching agents application induced neutrophils, macrophages and lymphocytes infiltration in the dental pulp. Tuckey HSD test showed that application of 37% phosphoric acid increased higher number of neutrophils, macrophages and lymphocytes significantly than 19% EDTA (p<0.05). Conclusion: The study suggested that 37% phosphoric acid induced higher number of the inflammatory cells than 19% EDTA.Latar belakang: Penggunaan bahan etsa seperti ethylene diamine tetraacetic acid (EDTA) dan asam fosfat pada sistem restorasi adhesif bertujuan untuk meningkatkan retensi bagi bahan restorasi, namun penggunaan bahan-bahan tersebut dapat menginduksi inflamasi pada pulpa. Respon inflamasi berfungsi untuk menghilangkan patogen, sel-sel atau jaringan yang rusak dan menginisiasi perbaikan. Netrofil dan makrofag adalah sel fagosit yang merupakan garis pertama pertahanan tubuh. Tujuan: Penelitian ini bertujuan untuk meneliti efek EDTA 19% dan asam fosfat 37% sebagai bahan etsa terhadap respon inflamasi pada pulpa gigi. Metode: Empat puluh lima ekor tikus Sprague Dawley jantan dibagi menjadi 3 kelompok. Permukaan oklusal gigi molar satu rahang atas dipreparasi menggunakan diamond round bur. Pada kelompok I kavitas diaplikasikan EDTA 19%, kelompok II diaplikasikan asam fosfat 37% dan kelompok III diaplikasikan akuades. Hewan coba dikorbankan pada hari ke-1, 3, 5, 7 dan 14 setelah aplikasi bahan etsa (n=3). Spesimen diproses secara histologis dan dicat dengan hematoksilin eosin. Hasil: Hasil ANOVA menunjukkan perbedaan yang bermakna (p<0,05) antar kelompok perlakuan, mengindikasikan bahwa aplikasi bahan etsa menyebabkan infiltrasi sel inflamasi pada pulpa, baik netrofil, makrofag dan limfosit. Hasil uji Tuckey HSD menunjukkan bahwa asam fosfat 37% menstimulasi infiltrasi sel netrofil, makrofag dan limfosit signifikan (p<0,05) lebih banyak dibanding EDTA 19%. Simpulan: Penelitian ini menunjukkan bahwa asam fosfat 37% menyebabkan infiltrasi sel inflamasi yang lebih banyak dibanding EDTA 19%.

The effect of CPP-ACP containing fluoride on Streptococcus mutans adhesion and enamel roughness Yulita Kristanti; Widya Asmara; Siti Sunarintyas; Juni Handajani
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 4 (2013): December 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Direct contact between the bleaching agent and the enamel surface results in demineralization, alteration in surface roughness and bacterial adhesion. Many studies try to minimize this side effect through different way. Purpose: The aim of this study was to determined the effect of Calcium Phospho Peptide-Amorphous Calcium Phosphate (CPP-ACP) containing fluoride application before and after bleaching procedure on the adhesion of S. Mutans and enamel roughness. Methods: The samples were 6 teeth which were divided into 4 groups, and each tooth was cut into four pieces. Group A and C were treated with CPP-ACP after bleaching, while group B and D were treated with CPP-ACP before and after bleaching. CPP-ACP used in group C and D was the one that contain fluoride. After treatment, all samples were sterilized, immersed in steril human saliva for one hour, then immersed into S. mutans suspension of 108 CFU. Samples were incubated overnight. On the next day the samples were put into steril BHI and vortexed for one minute to detach the bacteria. Fifteen ml BHI containing bacteria was poured into TYS agar then incubated 37°Cfor 48 hours. Bacterial colony was counted with colony counter. The SEM examination was done on all samples. Results: Application of desensitizing agent reduced the S.mutans adhesion significantly among groups (p<0.05) except between group A and C. SEM evaluation revealed significant differences among groups. Conclusion: The application of CPP-ACP containing fluoride before and after bleaching was effective to reduce the accumulation of S.mutans colony and enamel roughness.Latar belakang: Kontak langsung antara bahan bleaching dan permukaan enamel menyebabkan demineralisasi, perubahan kekasaran permukaan dan berpengaruh terhadap banyaknya bakteri Streptococcus mutans (S. mutans) yang melekat. Banyak peneliti mencoba meminimalkan efek samping ini dengan cara yang beragam. Tujuan: Penelitian ini bertujuan untuk meneliti efek aplikasi CPP-ACP mengandung fluor sebelum dan setelah bleaching terhadap adhesi S.mutans dan kekasaran enamel. Metode: Sampel penelitian adalah 6 buah gigi yang dibagi dalam 4 kelompok, kemudian masing-masing gigi dibelah menjadi 4 bagian. Kelompok A dan C diaplikasi dengan CPP-ACP setelah bleaching, sedang Kelompok B dan D diaplikasi CPP-ACP sebelum dan setelah bleaching. CPP-ACP yang digunakan pada kelompok C dan D adalah yang mengandung fluor. Setelah perlakuan, semua sampel disterilkan dan direndam dalam saliva steril, lalu direndam dalam suspensi S. mutans 108 CFU dan diinkubasi 24 jam. Hari berikutnya sampel dimasukkan dalam BHI steril, divortex 1 menit untuk melepaskan bakteri. Lima belas ml BHI yang berisi S. mutans tersebut diambil untuk dikultur dalam agar TYS dan diinkubasi 37°C selama 48 jam. Bakteri yang tumbuh dihitung dengan colony counter. Pemeriksaan SEM dilakukan untuk meneliti permukaan enamel. Hasil: Aplikasi CPP-ACP(F) menurunkan jumlah bakteri yang melekat pada enamel secara signifikan (p<0,05) pada semua kelompok, kecuali antara kelompok A dan C. Simpulan: Aplikasi CPP-ACP mengandung fluor sebelum dan sesudah bleaching efektif mengurangi akumulasi S. mutans dan kekasaran pada permukaan enamel.

Sifat fisik hidroksiapatit sintesis kalsit sebagai bahan pengisi pada sealer saluran akar resin epoxy (Physical properties of calcite synthesized hydroxyapatite as the filler of epoxy-resin-based root canal sealer) Ema Mulyawati; Marsetyawan HNES; Siti Sunarintyas; Juni Handajani
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 4 (2013): December 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: The filler addition to resin based sealers will enhance the physical properties of the polymer. Because of its biological properties, the synthetic hydroxyapatite (HA) has been proposed as filler for dental material such as composite resin. The calcite synthesized HA is the HA produced of calcite minerals that came from many Indonesian mining. Purpose: The aim of study was to determine the effect of different concentration of calcite synthesized HA as the filler of the epoxy-resin-based root canal sealer on the physical properties such as its contact angle, the film thickness and the microhardness. Methods: The crystal of the calcite synthesized hydroxyapatite with the size between 77.721-88.710 nm and the ratio of Ca/P 1.6886 were synthesized at Ceramic Laboratory, Mechanical Engineering, using wet method of hydrothermal microwave. The powders of the epoxy- resin were prepared by added the synthesized hydroxyapatite crystal in 5 different weight ratios (e.g.: HA-10%, HA-20%, HA-30%, HA-40% and HA-50%). Each of these was mixed with the paste of 3:1 ratio using spatula on a glass plate until homogen and then measuring the contact angle and the film thickness. Microhardness test was conducted after the mixture of experimental sealer was stored for 24 hrs at 37 oC to reach perfect polymerization. Results: All of contact angles were <90o and were not significantly different to each other (p= 0.510). All groups had a film thickness in accordance with ISO 6876 (<50 um) and with no statistical difference (p= 0.858). In the HA of 10%, 20%, 30% seen that the microhardness were increased, while in the HA-50% was decreased and in the HA-40% has the same microhardness to the control groups (HA-0%). Conclusion: Calcite synthesized HA as the filler did not affect contact angle and film thickness of the sealer. Microhardness of the epoxy-resin based sealer could be increased using maximum 30% of the calcite synthesized HA as the filler.Latar belakang: Penambahan bahan pengisi pada sealer berbahan dasar resin akan meningkatkan sifat fisik polimer. Karena sifat biologis bagus, hidroksiapatit (HA) sintetis digunakan sebagai bahan pengisi material kedokteran gigi seperti resin komposit. Hidroksiapatit sintesis kalsit merupakan HA yang hasilkan dari mineral kalsit berasal dari berbagai daerah pertambangan di Indonesia. Tujuan: Penelitian ini bertujuan untuk meneliti pengaruh berbagai konsentrasi HA sintesis kalsit sebagai bahan pengisi sealer berbahan dasar resin epoksi terhadap sifat fisiknya yaitu sudut kontak, ketebalan film dan kekerasan mikronya. Metode: Kristal HA sintesis kalsit yang berukuran 7,721-88,710 nm dengan rasio Ca/P 1,6886 diperoleh dari sintesis di Laboratorium Keramik, Teknik Mesin, Universitas Gadjah Mada menggunakan wet method dengan microwave hidrotermal. Serbuk resin epoksi dipersiapkan dengan menambahkan kristal HA sintesis kalsit dalam lima konsentrasi yang berbeda yaitu HA-10%, HA-20%, HA-30%, HA-40% dan HA-50% (dalam berat). Masing-masing serbuk diaduk dengan pasta resin epoksi dengan perbandingan 3:1 menggunakan spatula di atas glassplate hingga homogen, selanjutnya dilakukan pengukuran sudut kontak dan ketebalan film. Pengukuran kekerasan mikro dilakukan setelah sealer disimpan dalam inkubator 37 oC selama 24 jam sehingga mencapai polimerisasi sempurna. Hasil: Semua kelompok menunjukkan bahwa sudut kontak <90o dan menunjukkan tidak ada perbedaan yang signifikan (p=0,510). Semua kelompok menunjukkan bahwa ketebalan filmnya sesuai dengan ISO 6876 (<50 um) dan menunjukkan tidak ada perbedaan yang signifikan (p=0,858). Pada kelompok HA-10%, 20% dan 30% kekerasan mikronya meningkat, sedangkan pada HA-50% menurun dan HA-40% kekerasannya sama dengan kelompok kontrol (HA-0%). Simpulan: Penambahan hingga 50% HA sintesis kalsit sebagai bahan pengisi sealer resin epoksi tidak mempengaruhi sudut kontak dan ketebalan film. Kekerasan sealer dapat ditingkatkan dengan menambahkan HA sintesis kalsit maksimum hingga 30%.

Co-Authors Afifah Rufaida Alma Linggar Jonarta Annisa Rahmi Fauzia Antinah Latif, Antinah Anugerah Pekerti Astamurtiningrum Ardiny Andriani Aurita Siwi R Aurita Siwi R, Aurita Siwi Bambang Priyono Chynintia Dewi Aqmarina Dea Cahyani, Yessica Dhika Paramita Prameswari Dhika Paramita Prameswari, Dhika Paramita Dhinintya Hyta N Dhinintya Hyta N, Dhinintya Hyta Dhinintya Hyta Narissi Dyah Irnawati Ema Mulyawati Endah Kusumastuti Enggardipta, Raras Ajeng Evy Tri Utami Evy Tri Utami Evy Tri Utami Failasofia Failasofia Fery Setiawan Fery Setiawan Finsa Tisna Sari Finsa Tisna Sari, Finsa Tisna Fitri Setia Rahayu Haniastuti, Tetiana Hardono Jaya Lauson Heni Susilowati Heni Susilowati Heni Susilowati Icha Nofikasari Indah Fatikarini Islamy Rahma Hutami, Islamy Rahma Isti Endah Kurniwati Isti Endah Kurniwati, Isti Endah Juwita Raditya Ningsih Kristiyani Dwi M Kristiyani Dwi M, Kristiyani Dwi Lanagusti, Alfin Layung Sekar Prabarayi Lisdrianto Hanindriyo Lisdrianto Hanindriyo Lisna Kurnia Rezky Lukis, Prima Agusti Mahadna Aulia Rahmah Marsetyawan HNES Mochammad Imron Awalludin Moh. Khafid Mohammad Khafid Mufidana Azis Mufidana Azis, Mufidana Muhammad Nabil Pratama Nadia Rully Auliawati Nadie Fatimatuzzahro Norma Dias L Norma Dias L, Norma Dias Nuryono Nuryono Orienty, Fauzia Nilam Prakosa, Basma Rosandi Pramana Pananja Putra Pratama, I Wayan Gita Pribadi Santosa Rais Aliffandy Damroni Rebecca Azary Kuncoro Rebecca Azary Kuncoro, Rebecca Azary Regina TC. Tandelilin Regina TC. Tandelilin, Regina TC. Regina Titi Christinawati Tandelilin Rini Maya Puspita Ristini Asih Rizki Amelia Rizki Amelia Rizki Amelia Rizki Fadlilah Santoso, Aloysia Supartinah Sifra Kristina Hartono Singh, Malreen Kaur Harban Siti Fatimah Siti Sunarintyas Sri Widiati, Sri Stefany Elan Saktiyawardani Swastiana Eka Yunita Tri Ayu Hidayani Tri Ayu Hidayani Tri Endra Untara, Tri Endra Urfa Tabtila Virta Devi Kartika Putri Volanda Kusumaningsari Widya Asmara Widya Asmara Yoga, I Gede Krisna Merta Yosaphat Bayu Rosanto Yulita Kristanti

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