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EFFECT OF EXTRACT GEL GREEN TEA (Camelia Sinensis) ON NEUTROPHIL OF POST EXTRACORONAL BLEACHING RAT’S TEETH Kristanti, Yulita; Handajani, Juni; Untara, Tri Endra; Santosa, Pribadi
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.212-220

Abstract

Background: Free radicals produced by hydrogen peroxide after teeth bleaching which diffuse through the enamel and dentinal tubules can trigger inflammation of the pulp.  To overcome this negative side effect, exogenous antioxidants have been proposed.  This research was conducted to determine the effect of natural antioxidants, namely 5% and 10% green tea, which was applied on rat molars teeth that were bleached using 40% hydrogen peroxide. Methods: Fifteen molar teeth of male Wistar rats were bleached using 40% hydrogen peroxide on their occlusal surface. Furthermore, the control group (Group I) rinsed using warm distilled water, while in Group II and Group III, the teeth were rinsed using 5% and 10% green tea extract gel. Wistar rats were sacrificed on the fifth day after treatment, followed by hematoxylin-eosin staining. Histological examinations were observed under a light microscope using 400x magnification and the number of the neutrophil were counted. Result: One-way ANOVA test showed that there was a significant effect of green tea extract gel concentration on the number of neutrophils (p 0.05).  Post hoc test results showed that there were significant differences in all pairs of groups compared (p 0.05). Conclusion:  Group III which was treated using10% green tea extract gel has the lowest number of neutrophil compared to Group II which was treated using 5%  green tea extract hel and the control group.
Potensi ekstrak biji chia (Salvia hispanica L.) dalam menghambat pembentukan biofilm Streptococcus mutans ATCC 25175 in vitro Lanagusti, Alfin; Handajani, Juni; Haniastuti, Tetiana
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 10, No 1 (2024)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.100143

Abstract

Streptococcus mutans merupakan bakteri gram positif yang berperan sebagai faktor etiologi utama karies dan koloni primer dalam pembentukan biofilm rongga mulut. Biji chia mengandung flavonoid dan asam fenolik yang mampu menghambat pertumbuhan bakteri. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak etanol biji chia terhadap pembentukan biofilm S. mutans. Penelitian eksperimental laboratoris ini menggunakan metode uji dengan microtiter plate. Streptococcus mutans ATCC 25175 dikultur dalam BHI yang mengandung 1% sukrosa dan diberi perlakuan ekstrak biji chia 1,3%, 2,6%, 5,2%, klorheksidin glukonat 0,2% sebagai kontrol positif dan akuades sebagai kontrol negatif. Biakan bakteri diinkubasi selama 24 jam dalam 96-well microplate kemudian diwarnai dengan kristal violet 0,1% selama 15 menit. Densitas optik dibaca dengan microplate reader (λ = 540 nm). Data dianalisis dengan uji Kruskal-Wallis dan uji post-hoc U-Mann Whitney. Uji Kruskal-Wallis menunjukkan perbedaan signifikan (p < 0,05) rerata persentase daya hambat pembentukan biofilm S. mutans pada setiap kelompok perlakuan. Uji U-Mann Whitney menunjukan ekstrak etanol biji chia konsentrasi 1,3%, 2,6%, dan 5,2% memiliki kemampuan setara dalam pembentukan biofilm S. mutans meskipun masih lebih rendah dibandingkan klorheksidin 0,2%. Kesimpulan dari penelitian ini adalah ekstrak etanol biji chia mampu menghambat pembentukan biofilm S. mutans ATCC 25175.
Pengabdian Masyarakat tentang Peningkatan Kesehatan Gigi dan Mulut di Pondok Pesantren Ta'lim Sabilu Taubah Kecamatan Srengat Kabupaten Blitar Lukis, Prima Agusti; Setiawan, Fery; Prakosa, Basma Rosandi; Khafid, Mohammad; Handajani, Juni; Hanindriyo, Lisdrianto; Irnawati, Dyah; Susilowati, Heni; Sunarintyas, Siti
Jurnal Abdi Masyarakat Indonesia Vol 5 No 2 (2025): JAMSI - Maret 2025
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jamsi.1825

Abstract

Persepsi kebersihan gigi dan oral memiliki dampak besar pada harapan hidup di seluruh dunia. Namun, di negara-negara dengan pendapatan sedang, beban meningkat secara tidak proporsional. Prob-lema kesehatan gigi dan mulut di Indonesia merupakan problem yang mempengaruhi populasi sebanyak 57.6%. Studi ini adalah studi semi-eksperimental yang dilakukan pada 16 Desember 2024 di Ta’lim Sa-bilu Taubah Gas Iqdam, Blitar, Jawa Timur, Indonesia. Metode sosialisasi dilakukan secara offline (tatap muka) menggunakan campuran media presentasi (power point) dan booklet. Peserta sosialisasi dibagi menjadi dua bagian: pra-sosialisasi (pre-test) dan pasca-sosialisasi (post-test). Peserta sosialisasi diberi-kan pertanyaan untuk menilai pengetahuan dan persepsi peserta mengenai kesehatan gigi dan mulut pada saat sebelum dan sesudah sosialisasi. Pengetahuan peserta diklasifikasikan sebagai cukup, sedang, dan rendah dalam memahami kebersihan gigi dan mulut sebelum dan setelah sosialisasi. Skor rata-rata untuk peserta sebelum sosialisasi adalah 26,45, dan setelah sosialisasi menjadi 83,23. Hasil tersebut ber-beda bermakna dengan nilai-p 0.000 menggunakan uji statistik wilcoxon. Data ini menunjukkan bahwa terdapat peran positif dari dokter gigi dalam meningkatkan kesehatan gigi dan mulut pasien sehingga dokter gigi dapat berperan aktif untuk mendorong pasien lebih memperhatikan kesadaran kesehatan gigi dan mulut terutama bagi pondok pesantren Ta’lim Sabilu Taubah di Kabupaten Blitar.
PROMOSI KESEHATAN DENTAL: UPAYA MENINGKATKAN KEBERSIHAN MULUT MELALUI BUAH-BUAHAN LOKAL PADA MASYARAKAT DESA SRENGAT, BLITAR Moh. Khafid; Fery Setiawan; Juni Handajani; Lisdrianto Hanindriyo; Dyah Irnawati
DedikasiMU : Journal of Community Service Vol. 7 No. 2 (2025): DedikasiMU Juni
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/dedikasimu.v7i2.9889

Abstract

Kesadaran akan kesehatan gigi dan mulut mempunyai dampak yang signifikan terhadap peningkatan angka harapan hidup di seluruh dunia. Bebannya semakin tidak seimbang di negara-negara berpendapatan rendah dan menengah. Menurut Riskesdas 2018, permasalahan kesehatan gigi dan mulut di Indonesia sebesar 57,6%. Prevalensi karies di Provinsi Jawa Timur meningkat sebesar 54,22% pada tahun 2018. Peningkatan ini tidak terlepas dari konsumsi makanan instan dan minuman berenergi yang dapat menyebabkan suasana asam pada rongga mulut sehingga menyebabkan prevalensi risiko karies. Promosi kesehatan gigi ini dilakukan di Jemaah Ta’lim Sabilu Taubah Gus Iqdam, Blitar, Jawa Timur, Indonesia, pada tanggal 16 Desember 2024 untuk meningkatkan kebersihan mulut mereka melalui penggunaan buah-buahan lokal yang terdapat di Srengat, Blitar. Cara sosialisasi ini ditujukan kepada masyarakat yang menggunakan modus offline (langsung). Buah-buahan lokal yang banyak ditemui di Srengat Blitar adalah nanas. Buah nanas diklaim sebagai buah yang dapat meningkatkan self-cleansing. Disclosing agent akan membuat masyarakat mengetahui kategori kebersihan mulut mereka karena disclosing agent ini berfungsi sebagai penanda pada gigi mereka. Setelah mengetahui tentang kebersihan mulut, maka mereka dapat meningkatkan kebersihan mulut dengan mengonsumsi nanas sebagai bahan pembersih diri. Sosialisasi kesehatan gigi ini akan meningkatkan kebersihan mulut sehingga dapat menurunkan angka kejadian karies.
EDUKASI PEMANFAATAN BUAH NAGA MERAH SEBAGAI BAHAN DISCLOSING AGENT PADA MASYARAKAT SRENGAT, BLITAR Dea Cahyani, Yessica; Khafid, Mohammad; Handajani, Juni; Hanindriyo, Lisdrianto; Irnawati, Dyah; Susilowati, Heni; Sunarintyas, Siti
Jurnal Pengabdian Pendidikan Masyarakat (JPPM) Vol 6 No 2 (2025): Jurnal Pengabdian Pendidikan Masyarakat (JPPM), Vol 6 No 2 (Oktober 2025)
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH MUARA BUNGO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52060/jppm.v6i2.2845

Abstract

Using a disclosing agent to control plaque is one way to improve dental health and oral tissue. However, this material is not widely known to the public. Red dragon fruit, a mainstay commodity in Blitar Regency, contains betalain which can be used as an alternative disclosure agent. Therefore, the people of Blitar Regency need to be empowered to maintain dental and oral health regarding the potential of dragon fruit as an alternative natural ingredient. The aim is to provide education regarding the potential benefits of dragon fruit as a disclosure agent. The strategy used in this activity is dual media intervention by dentists, namely by using interactive lectures with PowerPoint media and booklet media to community groups in, Blitar Regency. To measure the level of knowledge of the target community, questionnaires were given before and after the activity, as well as checking the OHI-S index. The research results showed that the level of the OHI-S index was in the medium category, while the level of community knowledge showed an increase of 56.78% after being given counseling. This shows that providing dental and oral health education using PowerPoint media and booklet media has proven to be effective in increasing public knowledge. Therefore, it can be concluded that providing education on the use of red dragon fruit as a disclosing agent to the people of Blitar Regency has proven effective in increasing knowledge, so it is hoped that it can improve the community's dental and oral hygiene.
The Level of Saliva Glutathione in Moderate Gingivitis Patients Increases After Gargling with 5% Cosmos (Cosmos caudatus) Extract Singh, Malreen Kaur Harban; Tandelilin, Regina Titi Christinawati; Handajani, Juni
Journal of Indonesian Dental Association Vol 4 No 2 (2021): October
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Introduction: Gingivitis is an inflammation of the gums caused by bacterial plaque accumulation producing reactive oxygen species (ROS). Reactive oxygen species is a harmful by-products from aerobic metabolisms of mitochondria, that when accumulated can cause large variety of diseases. Antioxidants can counter ROS activities. Oxidative stress may occur due to an imbalance between ROS and antioxidants. Glutathione (GSH) is an antioxidant that protects cells against oxidative damage. Cosmos caudatus is rich in antioxidants due to its flavonoid and phenolic contents. Objective: The aim of this study was to detect the concentration of saliva GSH in moderate gingivitis patients after gargling with a solution containing 5% Cosmos (Cosmos caudatus) extract. Methods: Twenty subjects with moderate gingivitis were divided into two groups—the treatment and control group—by drawing lots in a randomized controlled trial. The treatment group gargled with 5% Cosmos extract whereas the control group gargled with Chlorhexidine 0.1%. Each subject was required to gargle for 60 seconds every morning and night, for five days consecutively. The GSH level was measured before and after gargling on the sixth day after treatment using a spectrophotometer with wavelength of 412 nm. Data was analyzed using an independent T-Test (p <0.05). Results: The study showed there was significant difference between the saliva GSH level of each group before and after gargling; moreover, after gargling with the solutions, no significant difference of saliva GSH was found when compared between the treatment group and the control group. Conclusion: The level of saliva GSH increases after gargling using 5% Cosmos extract and has the same effect with Chlorhexidine 0.1%.
EDUKASI PEMANFAATAN BUAH NAGA MERAH SEBAGAI BAHAN DISCLOSING AGENT PADA MASYARAKAT SRENGAT, BLITAR Dea Cahyani, Yessica; Khafid, Mohammad; Handajani, Juni; Hanindriyo, Lisdrianto; Irnawati, Dyah; Susilowati, Heni; Sunarintyas, Siti
Jurnal Pengabdian Pendidikan Masyarakat (JPPM) Vol 6 No 2 (2025): Jurnal Pengabdian Pendidikan Masyarakat (JPPM), Vol 6 No 2 (Oktober 2025)
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH MUARA BUNGO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52060/jppm.v6i2.2845

Abstract

Using a disclosing agent to control plaque is one way to improve dental health and oral tissue. However, this material is not widely known to the public. Red dragon fruit, a mainstay commodity in Blitar Regency, contains betalain which can be used as an alternative disclosure agent. Therefore, the people of Blitar Regency need to be empowered to maintain dental and oral health regarding the potential of dragon fruit as an alternative natural ingredient. The aim is to provide education regarding the potential benefits of dragon fruit as a disclosure agent. The strategy used in this activity is dual media intervention by dentists, namely by using interactive lectures with PowerPoint media and booklet media to community groups in, Blitar Regency. To measure the level of knowledge of the target community, questionnaires were given before and after the activity, as well as checking the OHI-S index. The research results showed that the level of the OHI-S index was in the medium category, while the level of community knowledge showed an increase of 56.78% after being given counseling. This shows that providing dental and oral health education using PowerPoint media and booklet media has proven to be effective in increasing public knowledge. Therefore, it can be concluded that providing education on the use of red dragon fruit as a disclosing agent to the people of Blitar Regency has proven effective in increasing knowledge, so it is hoped that it can improve the community's dental and oral hygiene.
Co-Authors Afifah Rufaida Alma Linggar Jonarta Annisa Rahmi Fauzia Antinah Latif, Antinah Anugerah Pekerti Astamurtiningrum Ardiny Andriani Aurita Siwi R Aurita Siwi R, Aurita Siwi Bambang Priyono Chynintia Dewi Aqmarina Dea Cahyani, Yessica Dhika Paramita Prameswari Dhika Paramita Prameswari, Dhika Paramita Dhinintya Hyta N Dhinintya Hyta N, Dhinintya Hyta Dhinintya Hyta Narissi Dyah Irnawati Ema Mulyawati Endah Kusumastuti Enggardipta, Raras Ajeng Evy Tri Utami Evy Tri Utami Evy Tri Utami Failasofia Failasofia Fauzia Nilam Orienty Fery Setiawan Fery Setiawan Finsa Tisna Sari Finsa Tisna Sari, Finsa Tisna Fitri Setia Rahayu Haniastuti, Tetiana Hardono Jaya Lauson Heni Susilowati Heni Susilowati Heni Susilowati Icha Nofikasari Indah Fatikarini Islamy Rahma Hutami, Islamy Rahma Isti Endah Kurniwati Isti Endah Kurniwati, Isti Endah Juwita Raditya Ningsih Kristiyani Dwi M Kristiyani Dwi M, Kristiyani Dwi Lanagusti, Alfin Layung Sekar Prabarayi Lisdrianto Hanindriyo Lisdrianto Hanindriyo Lisna Kurnia Rezky Lukis, Prima Agusti Mahadna Aulia Rahmah Marsetyawan HNES Mochammad Imron Awalludin Moh. Khafid Mohammad Khafid Mufidana Azis Mufidana Azis, Mufidana Muhammad Nabil Pratama Nadia Rully Auliawati Nadie Fatimatuzzahro Norma Dias L Norma Dias L, Norma Dias Nuryono Nuryono Prakosa, Basma Rosandi Pramana Pananja Putra Pratama, I Wayan Gita Pribadi Santosa Rais Aliffandy Damroni Rebecca Azary Kuncoro Rebecca Azary Kuncoro, Rebecca Azary Regina TC. Tandelilin Regina TC. Tandelilin, Regina TC. Regina Titi Christinawati Tandelilin Rini Maya Puspita Ristini Asih Rizki Amelia Rizki Amelia Rizki Amelia Rizki Fadlilah Santoso, Aloysia Supartinah Sifra Kristina Hartono Singh, Malreen Kaur Harban Siti Fatimah Siti Sunarintyas Sri Widiati, Sri Stefany Elan Saktiyawardani Swastiana Eka Yunita Tri Ayu Hidayani Tri Ayu Hidayani Tri Endra Untara, Tri Endra Urfa Tabtila Virta Devi Kartika Putri Volanda Kusumaningsari Widya Asmara Widya Asmara Yoga, I Gede Krisna Merta Yosaphat Bayu Rosanto Yulita Kristanti