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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Dentika Dental Journal MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Majalah Obat Tradisional Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry JIKG (Jurnal Ilmu Kedokteran Gigi) Journal of Indonesian Dental Association B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah DedikasiMU: Journal of Community Service Jurnal Abdi Masyarakat Indonesia JURNAL PENGABDIAN PENDIDIKAN MASYARAKAT (JPPM) Odonto dental journal

Juni Handajani

Universitas Gadjah Mada

Author-ID : 273943

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Pulpal inflammation after vital tooth bleaching with 38% hydrogen peroxide Ardiny Andriani; Juni Handajani; Tetiana Haniastuti
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 2 (2012): June 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: In-office vital tooth bleaching is a treatment to remove tooth stains. Tooth sensitivity is one of side effect commonly complained by patients receiving this treatment. Purpose: The aim of this study was to examine histological inflammatory cells infiltration of dental pulp after application of 38% H2O2 as a vital tooth bleaching agent. Methods: Under informed consent, a total of 15 premolars from 8 healthy subjects scheduled for orthodontic extraction were used in this study. Thirty eight percent H2O2 was applied on the buccal surface of the treated group. The treated teeth were extracted after 1 hour, 5, 8, and 15 days. All specimens were embedded in paraffin wax, sectioned serially and stained with Hematoxyllin Eosin. Histological specimens were then observed under a light microscope. Results: All treated groups showed a slight disorganization of odontoblasts layer and slight inflammation in the pulp tissue adjacent to the 38% H2O2 application site. The number of polymorphonuclear leukocytes (PMN) had increased significantly 1 hour after application of 38% H2O2 (p<0.05), while macrophages had significantly increased 5 days after the application (p<0.05). The most intense PMN and macrophages infiltration was found 5 days after the application and gradually decreased 8 days after application of38% H2O2. Conclusion: Application of 38% H2O2 as a vital tooth bleaching agent induces acute inflammation in human dental pulp; however, the inflammation will decrease 8 days after the application.Latar belakang: Perawatan pemutihan gigi vital metode in-office merupakan tindakan untuk menghilangkan pewarnaan pada gigi. Salah satu efek samping yang sering dikeluhkan oleh pasien yang menjalani perawatan ini adalah sensitivitas gigi. Tujuan: Penelitian ini bertujuan untuk mengamati infiltrasi sel inflamasi pada pulpa gigi setelah aplikasi H2O2 38% sebagai bahan pemutih gigi. Metode: Sampel penelitian ini berupa 15 gigi premolar yang berasal dari 8 subjek sehat yang akan melakukan pencabutan gigi untuk perawatan ortodontik. Seluruh subjek telah menandatangani informed consent. Hidrogen peroksida 38% diaplikasikan pada permukaan bukal gigi kelompok perlakuan. Gigi kemudian dicabut 1 jam, 5, 8, dan 15 hari setelah aplikasi H2O2 38%. Seluruh spesimen kemudian ditanam dalam parafin, dipotong secara serial dan diwarnai dengan Hematoxillin Eosin. Pengamatan preparat histologis dilakukan dengan menggunakan mikroskop cahaya. Hasil: Hasil penelitian ini menunjukkan gangguan pada lapisan odontoblas dan peradangan pada jaringan pulpa di bawah daerah aplikasi H2O2. Jumlah PMN meningkat secara signifikan (p<0,05) 1 jam setelah aplikasi H2O2 38% sedangkan jumlah makrofag meningkat secara signifikan 5 hari setelah aplikasi hidrogen peroksida 38%. Infiltrasi PMN dan makrofag paling banyak ditemukan 5 hari setelah aplikasi dan menurun secara bertahap 5 dan 8 hari setelah aplikasi H2O2 38%. Kesimpulan: Aplikasi H2O2 38% sebagai bahan pemutih gigi vital dapat menginduksi inflamasi akut pada pulpa gigi manusia, namun, inflamasi akan mereda 8 hari setelah aplikasi.

The effects of Curcuma zedoaria oil on high blood sugar level and gingivitis Juni Handajani; Dhinintya Hyta Narissi
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 2 (2015): June 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Hyperglycemia is a condition when blood sugar level is higher than normal. Hyperglycemia is also one of diabetes mellitus (DM) symptoms. Hyperglycemia has a correlation with the occurrence of periodontal disease. Curcuma zedoaria oil is known to decrease concentration of serum glucose. Purpose: This study was aimed to determine the effects of Curcuma zedoaria oil on high blood sugar level and gingivitis in rats. Method: This study used twenty-five male Wistar rats, divided into two groups, namely the treatment group and the control group. In the treatment group, fifteen rats were divided into three subgroups (each of which was induced with 10 μl/ml, 30 μl/ml and 50 μl/ml of Curcuma zedoaria oil). The control group was consisted often rats, divided into two subgroups, as the positive control group (induced with 10 mg/kg of Glibenclamide) and the negative control group (induced with propylene glycol). Streptozotocin (STZ) (Naclai tesque, Kyoto Japan) with a dose of 40 mg/kg was used to create hyperglycemia condition in those rats. Gingivitis was then made by using silk ligature in those hyperglycemia rats. Silk ligature was twisted at the margin of gingiva anterior mandibular incisors for seven days. After the rats had gingivitis, Curcuma zedoaria oil, glibenclamide and propylene glycol were orally administered for seven days. Their gingivitis condition was observed, and their blood sugar level was measured before and after the induction of STZ and during the treatment. The data obtained were analyzed by using Manova. Result: There were significant differences of blood sugar levels between the treatment group before and after the administration of Curcuma zedoaria oil and the positive control group (p<0.05). Healthy gingiva was then found in the treatment group and the positive control group. Conclusion: Curcuma zedoaria oil can decrease blood sugar level and gingivitis.

Streptococcus Alpha growth in gingivitis patient’s dental plaque after rinsing with green tea extract (Camellia Sinensis) Isti Endah Kurniwati; Juni Handajani; Regina TC. Tandelilin
Padjadjaran Journal of Dentistry Vol 19, No 1 (2007): March 2007
Publisher : Faculty of Dentistry Universitas Padjadjaran

Green tea (Camellia sinensis) has been widely known as a healthy drink since long time ago. One of the substances in green tea which can give health benefit is catechin, an antibacterial substance. The purpose of this research is to know the efficacy of rinsing with green tea extract to Streptococcus alpha growth on gingivitis patient’s dental plaque. The research subjects include 30 mild gingivitis patients, and these subjects are divided into two groups: treatment group (20 patients) and control group (10 patients). Ten patients of treatment group rinse their mouth with 0.25% green tea extract and 10 other patients with 0.5% green tea extract. Meanwhile, control group use Bactidol (0.1% Hexetidine). Rinsing the mouth is performed every morning and night for five days. Sampling is conducted on the first and sixth day. Before rinsing data is analyzed using ANOVA and the result shows a significant difference. ANAVA testing then is done using proportion value. ANAVA Testing result shows that there is no significant difference among the patients in the treatment group. This result indicates that the effect of rinsing with 0.25% and 0.5% green tea extract is equivalent to the control (0.1% Hexetidine) to inhibit S. alpha growth on mild gingivitis patient’s dental plaque. From these two concentrations, it has not been known which one is the most effective concentration to inhibit S. alpha growth on mild gingivitis patient.

RE-EPITELISASI LUKA SOKET PASCA PENCABUTAN GIGI SETELAH PEMBERIAN GEL GETAH PISANG RAJA (Musa sapientum L) Kajian histologis pada marmut (Cavia cobaya) Juwita Raditya Ningsih; Tetiana Haniastuti; Juni Handajani
JIKG (Jurnal Ilmu Kedokteran Gigi) Vol 2. No 1. 2019
Publisher : Muhammadiyah University Press

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ABSTRACTGingival epithelium has an important role in the protection of the gingival tissue from mechanical, physical, and chemical trauma also microbial invasion. Re-epithelialization is an important phase of post extraction socket healing. Previous study showed that banana (Musa sapientum L) latex containing tanin, saponin, flavanoid, vitamin C, and minerals i.e: kalium, magnesium, calcium induced re-epithelialization in skin wounds. The objective of the present research was to study the effectivity of banana latex gel in the re-epithelialization of post extraction socket in guinea pigs (Cavia cobaya).Fifty four guinea pigs were divided into 3 groups, there were negative control group, positive control group and banana latex gel group. Each group consisted of 18 guinea pigs. Mandibular left central incisive were extracted and CMC-Na 1% (negative control), iod-glycerin (positive control) and banana latex gel were applied into the wound socket. Guinea pigs were then sacrified at 1,3,5,7,14,24 day post extraction and processed for histological examination. The specimens were stained with hematoxilin eosin. Epithelial thickness was measured with optilab (µm). The result of Anova showed significant differences in epithelial thickness among groups (p0,05). Least Significant of Difference test showed significant differences (p0,05) at 3,5,7,14,24 day post extraction between banana latex gel group compared to negative control group and also at 7 and 24 day post extraction between banana latex gel group compared to positive control group. In conclusion, banana latex gel may induce re-epithelialization of post extraction socket. The effect of banana latex gel is similar to iod-glycerin.Key words: re-epithelialization, banana latex gel, tooth socket

Efek merokok konvensional dan elektrik terhadap kadar hormon kortisol saliva Virta Devi Kartika Putri; Heni Susilowati; Juni Handajani
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 6, No 1 (2020)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.76282

Rokok konvensional menghasilkan asap atau aerosol padat dan rokok elektrik menghasilkan aerosol cair. Nikotin dari kedua jenis rokok tersebut diduga dapat mempengaruhi kadar hormon kortisol dalam saliva melalui respon autonomic nervous system (ANS) yang disertai aktivasi hypothalamic-pituitary-adrenal axis. Tujuan penelitian ini adalah untuk mengetahui pengaruh antara merokok konvensional dibandingkan dengan elektrik terhadap kadar hormon kortisol dalam saliva. Subjek sebanyak 18 laki-laki perokok terdiri dari 9 perokok konvensional dan 9 perokok elektrik, usia 2030 tahun. Pengambilan sampel saliva pada pukul 12.00 WIB. Uji kadar kortisol dalam saliva menggunakan ELISA kit (RnD Systems) dengan Panjang gelombang 450 nm. Perbandingan rerata kadar kortisol saliva perokok konvensional dan perokok elektrik dianalisis dengan menggunakan uji t tidak berpasangan. Hasil penelitian mengindikasikan bahwa kadar hormon kortisol dalam saliva perokok konvensional lebih tinggi daripada perokok elektrik, meskipun perbedaan tersebut tidak bermakna (p>0,05). Disimpulkan bahwa tidak terdapat perbedaan antara kadar kortisol saliva perokok konvensional dengan perokok elektrik.

EFEK EKSTRAK SAMBILOTO (Andrographis Paniculata) TERHADAP JUMLAH SEL INFLAMASI PADA MODEL PERIODONTITIS Fauzia Nilam Orienty; Juni Handajani; Tetiana Haniastuti
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 2, Nomor 1, Juni 2015
Publisher : Universitas Baiturrahmah

Periodontitis merupakan infeksi kronis pada jaringan periodontal yang disebabkan oleh interaksi antara bakteri periodonpatogen dengan sistem pertahanan tubuh. Antiinflamasi non steroid (AINS) adalah salah satu golongan obat yang digunakan pada kasus periodontitis. Konsumsi AINS dalam jangka waktu panjang memberikan efek samping seperti gangguan pada gastrointestinal. Sambiloto (Andrographis paniculata) merupakan tanaman obat yang mempunyai bahan aktif berupa andrographolide yang memiliki efek antiinflamasi dengan cara menghambat migrasi sel inflamasi. Penelitian ini bertujuan untuk mengkaji efek ekstrak sambiloto terhadap jumlah sel inflamasi pada kasus periodontitis yang diinduksi pada tikus. Penelitian ini menggunakan 64 tikus Wistar jantan. Periodontitis pada tikus diinduksi dengan cara pemasangan silk ligature pada daerah subgingiva gigi anterior rahang bawah selama 14 hari. Setelah 14 hari, ligasi dilepaskan dan tikus dari masing-masing kelompok diberi perlakuan ekstrak sambiloto 600 mg/kg BB (kelompok I), ekstrak sambiloto 900 mg/kg BB (kelompok II), aspirin (kelompok III) dan saline (kelompok IV) secara per oral. Tikus didekapitasi pada hari ke-1, -3, -5 dan -7 setelah perlakuan. Spesimen didekalsifikasi menggunakan EDTA 0,1M, ditanam pada parafin dan dilakukan pemotongan. Pengecatan hematoksilin eosin dilakukan untuk menghitung jumlah sel inflamasi. Hasil uji Anava dua jalur menunjukkan terdapat perbedaan bermakna (p<0,05) antar kelompok, mengindikasikan bahwa ekstrak sambiloto berpengaruh terhadap jumlah infiltrasi sel inflamasi. Hasil uji LSD memperlihatkan bahwa ekstrak sambiloto 900 mg/kg BB lebih efektif dalam menurunkan jumlah sel netrofil dan makrofag dibandingkan dengan ekstrak sambiloto dosis 600 mg/kg BB, aspirin dan saline. Kesimpulan dari penelitian ini bahwa ekstrak sambiloto 900 mg/kg BB dapat menurunkan jumlah sel inflamasi pada tikus yang diinduksi periodontitis.

37% Phosphoric Acid Induced Stronger Matrix Metalloproteinase-8 Expression of the Dental Pulp than 19% Ethylene Diamine Tetraacetic Acid Nadie Fatimatuzzahro; Tetiana Haniastuti; Juni Handajani
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

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Etching agents such as ethylene diamine tetraacetic acid (EDTA) and phosphoric acid which are widely used in adhesive restoration system aimed to increase for retention of restorative materials, may act a chemical irritant that induce inflammation of dental pulp. Inflammation is a body response against irritant and infectious agents. Matrix metalloproteinase-8, the major collagenolytic enzyme, degrades collagen type 1. This enzyme is expressed in low level in normal condition, however, the expression will increase during inflammation. The purpose of the present research was to study the effect of 19% EDTA and 37% phosphoric acid application as an etching agents on the MMP-8 expression of dental pulp. Forty-five male Sprague Dawley rats were divided into 3 groups. Cavity preparation was made on the occlusal surface of maxillary first molar using a round diamond bur. 19% EDTA, 37% phosphoric acid, and distilled water were applied on the surface of the cavity of the teeth in group I, II, and III subsequently. The cavity then filed by glass ionomer cements. The rats were sacrified at 1, 3, 5, 7, and 14 days after the treatment (n=3 for each day). The specimens were then processed histologically. Immunohistochemical (IHC) analysis was performed using rabbit anti rat MMP-8 polyclonal antibody to examine MMP-8 expression and HE (Hematoxylen Eosin) staining to observe the number of macrophages. The results showed 37% phosphoric acid application induced stronger expression of MMP-8 and higher number of macrophages than 19% EDTA. The strongest expression of MMP-8 seems on 5 days after the treatment where the highest number of macrophages were also found.

Effect of zinc oxide nanoparticle concentration coated on acrylic resin upon surface roughness and abrasion resistance Yoga, I Gede Krisna Merta; Harsini, Harsini; Sunarintyas, Siti; Handajani, Juni; Nuryono, Nuryono; Irnawati, Dyah
Majalah Kedokteran Gigi Indonesia Vol 10, No 1 (2024): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.92000

Acrylic resin is the material most often used as a base for dentures; however, acrylic resin has shortcomings in its biological properties as it does not have antimicrobial properties. ZnO is added to acrylic resin because of its antimicrobial properties. The aim of this research is to determine the effect of the concentration of ZnO nanoparticles as a heat polymerization acrylic resin coating on the coating’s resistance to abrasion. A total of 24 acrylic resin samples were made, each measuring 13 x 13 x 2 mm. The acrylic resin was coated with 3-(trimethoxysilyl)propyl methacrylate), then with ZnO nanoparticles with varying concentrations of 2.5%, 5%, and 7.5% in ethanol. ZnO nanoparticle coating was applied using the dip coating method. Subsequently, the samples were subjected to an initial roughness test, abrasion test, and final roughness test. The data obtained were analyzed using one-way ANOVA and LSD(0.05). The results showed that the mean change in roughness (μm) and standard deviation for groups I to IV were 0.11 ± 0.13, 0.08 ± 0.33, 0.1 ± 0.12, and 0.19 ± 0.15, respectively. The results of the one-way ANOVA test showed that there was no significant difference in the roughness after the abrasion test (p > 0.05). Thus, zinc oxide nanoparticles can reduce surface roughness and are resistant to surface abrasion.

Effectiveness of dental and oral health counseling using traditional folklore video media on knowledge in 8-9-year-old children Pratama, I Wayan Gita; Widiati, Sri; Priyono, Bambang; Santoso, Aloysia Supartinah; Handajani, Juni; Hanindriyo, Lisdrianto
Majalah Kedokteran Gigi Indonesia Vol 10, No 2 (2024): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.96179

Oral health problems among elementary school children are still very common. One of the contributing factors is behavior in maintaining oral hygiene. Behavior is established from knowledge which will then stimulate changes in attitudes and practice. Efforts to enhance knowledge in children can be achieved through counseling. This study aimed to determine the effectiveness of oral health counseling using traditional folklore video media on knowledge in children aged 8-9 years. This was quasi-experimental research with a two group pre-test and post-test design. Sampling was conducted using multistage cluster random sampling with a total sample of 112 children aged 8-9 years in elementary schools. The sample was divided into 2 groups: 56 children using traditional folklore video as the intervention group and 56 children using conventional video as the control group. Knowledge variables were measured using a questionnaire; most of the data were not normally distributed so data analysis was carried out using the Mann-Whitney U Test. The results of the difference analysis using the Mann-Whitney U Test at pre-post 2 showed a significant increase in knowledge in both groups. The median knowledge score of the traditional folklore video group showed a greater increase compared to the conventional video group by 13 (6.6 - 20) (p < 0.001). This study concluded that oral health counseling using traditional folklore video is more effective in increasing oral health knowledge in children aged 8-9 years compared to conventional video media.

EFFECT OF EXTRACT GEL GREEN TEA (Camelia Sinensis) ON NEUTROPHIL OF POST EXTRACORONAL BLEACHING RAT’S TEETH Kristanti, Yulita; Handajani, Juni; Untara, Tri Endra; Santosa, Pribadi
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.212-220

Background: Free radicals produced by hydrogen peroxide after teeth bleaching which diffuse through the enamel and dentinal tubules can trigger inflammation of the pulp. To overcome this negative side effect, exogenous antioxidants have been proposed. This research was conducted to determine the effect of natural antioxidants, namely 5% and 10% green tea, which was applied on rat molars teeth that were bleached using 40% hydrogen peroxide. Methods: Fifteen molar teeth of male Wistar rats were bleached using 40% hydrogen peroxide on their occlusal surface. Furthermore, the control group (Group I) rinsed using warm distilled water, while in Group II and Group III, the teeth were rinsed using 5% and 10% green tea extract gel. Wistar rats were sacrificed on the fifth day after treatment, followed by hematoxylin-eosin staining. Histological examinations were observed under a light microscope using 400x magnification and the number of the neutrophil were counted. Result: One-way ANOVA test showed that there was a significant effect of green tea extract gel concentration on the number of neutrophils (p 0.05). Post hoc test results showed that there were significant differences in all pairs of groups compared (p 0.05). Conclusion: Group III which was treated using10% green tea extract gel has the lowest number of neutrophil compared to Group II which was treated using 5% green tea extract hel and the control group.

Co-Authors Afifah Rufaida Alma Linggar Jonarta Annisa Rahmi Fauzia Antinah Latif, Antinah Anugerah Pekerti Astamurtiningrum Ardiny Andriani Aurita Siwi R Aurita Siwi R, Aurita Siwi Bambang Priyono Chynintia Dewi Aqmarina Dea Cahyani, Yessica Dhika Paramita Prameswari Dhika Paramita Prameswari, Dhika Paramita Dhinintya Hyta N Dhinintya Hyta N, Dhinintya Hyta Dhinintya Hyta Narissi Dyah Irnawati Ema Mulyawati Endah Kusumastuti Enggardipta, Raras Ajeng Evy Tri Utami Evy Tri Utami Evy Tri Utami Failasofia Failasofia Fauzia Nilam Orienty Fery Setiawan Fery Setiawan Finsa Tisna Sari Finsa Tisna Sari, Finsa Tisna Fitri Setia Rahayu Haniastuti, Tetiana Hardono Jaya Lauson Heni Susilowati Heni Susilowati Heni Susilowati Icha Nofikasari Indah Fatikarini Islamy Rahma Hutami, Islamy Rahma Isti Endah Kurniwati Isti Endah Kurniwati, Isti Endah Juwita Raditya Ningsih Kristiyani Dwi M Kristiyani Dwi M, Kristiyani Dwi Lanagusti, Alfin Layung Sekar Prabarayi Lisdrianto Hanindriyo Lisdrianto Hanindriyo Lisna Kurnia Rezky Lukis, Prima Agusti Mahadna Aulia Rahmah Marsetyawan HNES Mochammad Imron Awalludin Moh. Khafid Mohammad Khafid Mufidana Azis Mufidana Azis, Mufidana Muhammad Nabil Pratama Nadia Rully Auliawati Nadie Fatimatuzzahro Norma Dias L Norma Dias L, Norma Dias Nuryono Nuryono Prakosa, Basma Rosandi Pramana Pananja Putra Pratama, I Wayan Gita Pribadi Santosa Rais Aliffandy Damroni Rebecca Azary Kuncoro Rebecca Azary Kuncoro, Rebecca Azary Regina TC. Tandelilin Regina TC. Tandelilin, Regina TC. Regina Titi Christinawati Tandelilin Rini Maya Puspita Ristini Asih Rizki Amelia Rizki Amelia Rizki Amelia Rizki Fadlilah Santoso, Aloysia Supartinah Sifra Kristina Hartono Singh, Malreen Kaur Harban Siti Fatimah Siti Sunarintyas Sri Widiati, Sri Stefany Elan Saktiyawardani Swastiana Eka Yunita Tri Ayu Hidayani Tri Ayu Hidayani Tri Endra Untara, Tri Endra Urfa Tabtila Virta Devi Kartika Putri Volanda Kusumaningsari Widya Asmara Widya Asmara Yoga, I Gede Krisna Merta Yosaphat Bayu Rosanto Yulita Kristanti

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