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All Journal INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni AGRITROP Agrikultura Jurnal Ilmu Dasar e-Journal Pustaka Kesehatan Berkala Ilmiah Pertanian UNEJ e-Proceeding Jurnal Penelitian Pertanian Terapan Panrita Abdi - Jurnal Pengabdian pada Masyarakat Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Food Science and TechnologyJournal Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Karinov Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Transformasi BIOVALENTIA: Biological Research Journal Jurnal Ilmiah Buana Bastra: Bahasa, Susastra, dan Pengajarannya JIMAKUKERTA Semanggi: Jurnal Pengabdian kepada Masyarakat PAPUMA: Journal of Comunnity Services
TRI HANDOYO
Fakultas Pertanian Universitas Jember
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : UNEJ e-Proceeding

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Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Restanto, Didik Pudji; ., Slameto; Kriswanto, Budi; Addy, Hardian Susilo; Handoyo, Tri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.  It was greatly reduces the quality and production in the world such as India.  The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA  TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).  The CBSD RNA concentration around 55,2ng/ul.  The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention  (72oC for 1 min).  The results showed a single band of about 380 bp which is the  distance between the two primers were tested.  The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR
Co-Authors Ahdatu Uli Khikamil Maulidiya Ahmad Ilham Tanzil Andari, Syamsiyah Yuli Dwi Arifin, Muhammad Reza Arum, Ayu Puspita Azmi Saleh Azmia, Azmia Mar'atul Latipah Bambang Sugiharto Bambang Sugiharto Banun Kusumawardani Budi Kriswanto, Budi Candra Prayoga, Mohammad Damayanti, Dwi Putri Oktavia Dwi Putri Oktavia Damayanti Dwika Nano Hariyanto Efsari, Aqilla Nur Fanata, Wahyu Indra Duwi Fariza Oktaviani Hakim, Mohammad Sulton Hakim, Saila Najma Haq, Ahmad Thoriq Izzul HARDIAN SUSILO ADDY Hopipah, Opi Irma Novita Sari Irza Guari Syah Fitri Juhardi, Ujang Kacung Hariyono Kartikasari, Subekah Nawa Khamidah, Khusna Kiki Ulfaniah Kiki Ulfaniah Laily Ilman Widuri Laily Ilman Widuri, Laily Ilman Latif, Arkanudin Lenny Widjayanthi Mahros Darsin Marcus dos Santos Soares MISWAR - Mochammad Amrun Hidayat Mohammad Ali Mudhor Mohammad Ubaidillah Muhammad Hazmi Muhammad Imron Rosadi Muhammad Nur Thoriq Alfy Muhammad Syauqi Munandar, Denna Eriani Nasti, Elyanisa Maharani Nisrina, Sofia Bunga Parawita Dewanti Parawita Dewanti Pratiwi, Helti Anggiana Pudji Restanto, Didik Putu Gita Maya Widyaswari Mahayasih Rahadiyan, Aristyo Ratnasari, Tri Restanto, Didik Restanto, Didik Pudji Rizka Amalia Rony, Zahara Tussoleha Rudi Joelijanto Saputra, Tio Eka Saputro Gumelar, Bachtiar Sholeh Avivi Sigit Soeparjono Slameto Slameto Slameto Slameto SRI AYU MADE Sri Hartatik Suryo Prakoso, Suryo Tri Agus Siswoyo Tri Ratnasari Tri Wahyu Saputra Ubaidillah, Mohammad Umar Santoso Ummi Sholikhah Veronenci Yuliarbi Farlisa Wachju Subchan Wulanjari, Distiana Yani Corvianindya Rahayu Yugistia Bima, Rafi Yuli Witono Zahratus Sakdiyah Zahratus Sakdiyah Zaiyin Rizky Ageng Maulidia