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All Journal Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Aceh Journal of Animal Science Jurnal Medik Veteriner JURNAL PENDIDIKAN TAMBUSAI NERSMID: Jurnal Keperwatan dan Kebidanan Journal of Parasite Science Conservative Dentistry Journal Jurnal Kedokteran Hewan
Heni Puspitasari
Institute Of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Toxoplasma Oocyst Contamination in Water Sources in Goat-Sheep Farming In Sidoarjo District Mufa, Ramy Inas Mahirah; Eka Pramyrtha Hestianah; Lucia Tri Suwanti; Hani Plumeriastuti; Widya Paramita Lokapirnasari; Tony Hartono; Rina Vitriasari; Retno Yuli; Galaxy Guardian; Heni Puspitasari; Mufasirin
Journal of Parasite Science Vol. 9 No. 1 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i1.67669

Abstract

Toxoplasmosis is one of the zoonotic diseases caused by the protozoa Toxoplasma gondii. The disease infects warm-blooded animals including humans. The purpose of this study was to determine the contamination of Toxoplasma oocysts in water sources in goat-sheep farms in Sidoarjo Regency. A total of 63 water samples were used for the study. Water samples were taken from 5 sub-districts that have many goat-sheep farms, each sub-district has 2 villages, with each village having 6-7 samples. The examination used the floating method and was examined under a microscope with a magnification of 400x. The results of the examination found 21 samples of Toxoplasma gondii oocysts or 33.33% (21/63). The morphology of T.gondii oocysts is round, ovoid or ellipsoid with sizes varying between 8.02 - 12.72 micrometers. Preventive measures are needed by treating water before giving it to livestock.
Pengembangan Media Pembelajaran Berbasis Infografis Digital untuk Materi Struktur dan Kaidah Teks Prosedur Kelas XI SMA Kharisma, Yulan Ayu; Citraningrum, Dina Merdeka; Sulistyawati, Endah; Puspitasari, Heni; Putera, Shufi Syahbana; Krisdianti, Cucuk; Agustiningsih, Linda
Jurnal Pendidikan Tambusai Vol. 9 No. 2 (2025): Agustus
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v9i2.27463

Abstract

Penelitian ini bertujuan untuk mengembangkan media pembelajaran berbasis infografis digital pada materi struktur dan kaidah teks prosedur untuk siswa kelas XI SMA. Pengembangan media dilakukan melalui tahapan analisis kebutuhan, desain, pengembangan, implementasi, dan evaluasi. Hasil pengembangan menunjukkan bahwa infografis digital mampu menyajikan materi secara visual, ringkas, dan menarik sehingga memudahkan siswa dalam memahami konsep struktur serta kaidah kebahasaan teks prosedur. Uji coba terbatas menunjukkan peningkatan keterlibatan dan hasil belajar siswa setelah menggunakan media ini. Dengan demikian, media pembelajaran berbasis infografis digital dapat menjadi alternatif efektif dalam mendukung proses pembelajaran Bahasa Indonesia, khususnya pada materi teks prosedur, serta relevan untuk diterapkan di lingkungan pendidikan yang semakin digital.
Detection of Anti-Müllerian Hormone (AMH) mRNA in Serum and Ovarian Tissue of Local Indonesian Cattle Using EvaGreen-based RT–qPCR Dikman, Dicky Mohammad; Puspitasari, Heni; Waluyo, Seagames; Kurniawan, Muhammad ‘Ahdi; Supriyadi, Supriyadi; Srianto, Pudji; Luqman, Epy Muhammad; Suprayogi, Tri Wahyu; Madyawati, Sri Pantja; Safitri, Erma; Lestari, Tita Damayanti
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.382-397

Abstract

Anti-Müllerian hormone (AMH) has been identified as a potential biomarker for assessing ovarian reserve and reproductive capacity in cattle, with high heritability and relatively stable expression. However, studies detecting AMH mRNA in bovine serum remain scarce due to the matrix’s inherently low RNA yield, susceptibility to degradation, and potential presence of PCR inhibitors. This study provides an exploratory validation of AMH mRNA detection in serum — a challenging matrix compared with ovarian tissue — using EvaGreen-based reverse transcriptase–quantitative PCR (RT–qPCR). Specific primers for the AMH and β-actin genes were designed in silico and validated through melting curve analysis and linearity testing. The results showed high amplification efficiency (AMH: 100.2%, R² = 0.994; β-actin: 109.1%, R² = 0.996), with specific amplification of both targets. AMH detection in serum samples was successful in some samples, while the β-actin gene was consistently amplified as a reference gene. Despite the low RNA quality from serum and the presence of organic contaminants, the method demonstrated its feasibility for detecting AMH transcripts in a minimally invasive manner. Physiologically, AMH levels positively correlate with antral follicle count, superovulation success, and embryo quality, and are sensitive to heat stress and other environmental factors. These findings provide a foundational basis for developing molecular diagnostic approaches based on AMH gene expression in cattle reproductive management programs and support the future development of efficient, accurate, and context-specific biomolecular-assisted selection technologies for tropical livestock systems.
Prevalence of Hymenolepis nana in wild rats in Surabaya City, East Java Dwi Putri Rahmawati; Suwanti, Lucia Tri; Mufasirin; Endang Suprihati; Poedji Hastutiek; Kusnoto; Heni Puspitasari; Alasrorik, Muhammad Hizbulloh
Journal of Parasite Science Vol. 9 No. 2 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i2.65448

Abstract

Hymenolepiasis is one of the neglected zoonotic diseases in humans, caused by the cestodes Hymenolepis nana and Hymenolepis diminuta. Its definitive hosts are rodents (mostly rats). This study aims to identify the morphology both microscopically and macroscopically. This research is a laboratory exploratory research and the sampling method uses purposive sampling technique. The samples used in this study amounted to 100 samples of wild rats that had been trapped and then necropsied, worms obtained from the digestive tract were stained with dark red stain and observed with a microscope. The results showed that microscopic morphological examination showed that the anterior part of the H. nana worm had hooks and crowns. The overall prevalence of H. nana worms was found to be 18% (18/100). The prevalence of H. nana in wild rats in Surabaya City in Rattus tanezumi was 16% (12/77). Rattus novergicus in Surabaya area with positive samples was 26% (6/23). Morphology in H. nana worms has a distinctive difference. The anterior part of H. nana has hooks that surround the rostellum, the anterior part has a ‘crown of hooks’.
Detection of Cryptosporidium spp. in Wild Rats (Rattus spp.) in Surabaya, East Java Pratama, Ponasari Galuh; Suprihati, Endang; Suwanti, Lucia Tri; Mufasirin; Hastutiek, Poedji; Setiawan, Boedi; Puspitasari, Heni; Indasari, Elly Nur
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.71-78

Abstract

Cryptosporidiosis is a waterborne zoonosis caused by Cryptosporidium, which is a parasite that causes infection of the small intestine and leads to acute diarrhea in both humans and animals. Wild rats (Rattus spp.) are known to carry many zoonotic pathogens including Cryptosporidium. Given their close proximity to humans in urban environments, the likelihood of disease transmission is high. This study aims to detect the Cryptosporidium spp. infection in wild rats in Surabaya City, East Java Province. Intestinal fecal samples were collected using necropsy. The flotation test and Ziehl-Neelsen stain were used to observe the presence of Cryptosporidium under the microscope. The results showed that 69 samples were positive for Cryptosporidium spp. in the oocyst stage, which appeared dark pink with a clear cavity and the size of the oocysts ranged from 2-6 μm. The high incidence of cryptosporidiosis in wild rats is a potential health threat to both animals and humans.
PHYLOGENETIC ANALYSIS AND VIRULENCE-CODING GENES CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM LAYING HEN BRAIN WITH TETELO SYMPTOM Sarah, Safira Iqlima; Nugroho, Christian Marco Hadi; Kurnia, Ryan Septa; Wardhana, Dhandy Koesoemo; Puspitasari, Heni
Jurnal Kedokteran Hewan Vol 13, No 3 (2019): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i3.14493

Abstract

Newcastle disease (ND) is an acute poultry disease caused by Paramyxovirus group. It has characteristic neurological symptoms, called torticollis. The molecular assay to find out the presence of viral genes in the brain can be an option in detecting ND virus infections since it penetrates brain barrier system. The purpose of this study was to identify ND viruses in the brain of chickens with torticollis symptoms, to analyze its phylogenetic and to characterize its virulence genetic code. Samples used were 12 dead chickens with historically had torticollis symptoms, obtained from poultry farms at several areas in West Java and Banten. Chicken brains were prepared for reverse transcriptasepolymerase chain reaction (RT-PCR) test. All positive samples then sequenced to obtain its nucleotide sequences from some of Fusion (F) genes analyzed its phylogenetic by comparing with Indonesian ND isolate virus from GenBank using Mega X software. The results of RT-PCR test showed that only one sample (Virus MSL.03) contained genes of ND virus. Based on homology tests and phylogenetic analysis, the virus belonged to subgenotype VIIh with an identical level of 95.34-95.86% when compared to several isolates from Indonesia. The MSL.03 ND virus has 112RRRKRF117 pattern in F0 indicatest its virulent category.
Co-Authors Agung Sosiawan Agustiningsih, Linda Ahmad Yudianto Ahmad Yudianto Aksono HP., Eduardus Bimo Alasrorik, Muhammad Hizbulloh andi jayawardhana Andi Jayawardhana Arif Rahman Nurdianto Boedi Setiawan Budi Koestoro Caswita Caswita Christian Marco Hadi Nugroho Citraningrum, Dina Merdeka Dewi Purwatiningsih Dian Agustin Wahjuningrum Dikman, Dicky Mohammad DWI PUTRI RAHMAWATI Eka Pramyrtha Hestianah Endang Suprihati Erma Safitri Fauziah Fitri Hernanto Fery Setiawan Galaxy Guardian Hani Plumeriastuti Hanna Harnida, Hanna Indasari, Elly Nur jayawardhana, andi Jenny Sunariani Kharisma, Yulan Ayu Krisdianti, Cucuk Kurnia, Ryan Septa Kurniawan, Muhammad ‘Ahdi Kusnoto Laura Wihanto Lucia Suwanti, Lucia Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Meles, Dewa Ketut Mufa, Ramy Inas Mahirah Mufasirin Nugroho, Christian Marco Hadi Nurdianto, Arif Rahman Poedji Hastutiek Pratama, Ponasari Galuh Pudji Srianto Puput, Sesa Putera, Shufi Syahbana Rahadju Ernawati Retno Yuli Rina Vitriasari Ryan Septa Kurnia Safira Iqlima Sarah Sarah, Safira Iqlima Seagames Waluyo, Seagames Septian Hakim Susantoputro Setiawan Koesdarto Setyabudi Setyabudi Sri Pantja Madyawati, Sri Pantja Sulistyawati, Endah Supriyadi Supriyadi Swadheena Patro Tedyanto, Cecilia Putri Tita Damayanti Lestari Tony Hartono Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wardhana, Dhandy Koesoemo Waworuntu, Gladdy L. Widya Paramita Lokapirnasari Yuliana Praptiwi, Yuliana Yunus, Muchammad