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All Journal Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Indonesian Journal of Chemistry Jurnal Perikanan Universitas Gadjah Mada Journal of Tropical Crop Science Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Pusat Inovasi Masyarakat Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Bambu Laut: Jurnal Pengabdian Masyarakat
Sugeng Heri Suseno
2Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Kelautan, Institut Pertanian Bogor Jalan Agatis Lingkar Kampus Akademik IPB Telepon 0251-8622915 Faks. 0251-8622916, Bogor 16680
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Social Sciences

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Journal : Journal of Fisheries

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Antioxidant Activity and Potential Bioactive Peptides from Skin Protein Hydrolysate of Yellowfin Tuna (Thunnus albacares) Rachman, Sitti Hardiyanti; Santoso, Joko; Suseno, Sugeng Heri
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.41625

Abstract

Highlight Research Immersion of tuna skin using NaHCO3 at low temperatures effectively reduces the fat content. The DH value, MW, and amino acid composition are in line with the antioxidant activity of tuna skin hydrolysate. The ABTS method showed the highest antioxidant activity in tuna skin hydrolysate. FPH of tuna skin shows functional group characteristics and amino acid composition that are similar to collagen hydrolysate products. FPH of tuna skin has the potential as a source of antioxidants, ACE inhibitors, and antifibrinolytics. Abstract The tuna fillet industry produces abundant skin by-products with high protein, which has the potential as a raw material for fish protein hydrolysate (FPH) for a source of bioactive peptides. Exploration of bioactive peptides from fish skin is generally from hydrolyzed gelatin and collagen. The study aimed to produce FPH directly from tuna skin as an antioxidant and identify potential bioactive peptides. The research began by defatting using multiple concentrations of NaHCO3 and immersion times. The defatted fish skin was produced as FPH by enzymatic hydrolysis method using different papain enzyme concentrations and hydrolysis times. The selected treatments were assessed for antioxidant activity and bioactive peptides. The results showed that the defatting process using a 0.50% NaHCO3 for 30 minutes generated the highest reducing fat content in value was 80.53%. Using papain enzyme gave a significant effect on the DH of FPH, with value was 29.72-67.64%. Therefore, FPH obtained from different concentrations for 4 hours was chosen to characterize the antioxidant activity and bioactive peptide. Hydrolysis using 5% enzyme papain showed the highest antioxidant activity of DPPH, ABTS, and reducing power with values of 0.965 mg/mL, 0.495 mg/mL, and 0.415 absorbances, respectively. FPH possesses a molecular weight of 10.15-48.50 kDa. Functional groups detected were amides A, B, I, II, and III. Glycine, proline, and arginine became amino acids dominant of FPH. Based on the diversity of biopeptide compounds, several biological function candidates were detected, namely antioxidants, ACE inhibitors, and antifibrinolytics which have the potential to be used as nutraceutical products.
Co-Authors . Dahri . Uju Ade Wachjar Adenia Cahyatie Aprillia Adha, As Syaffa Amalia Adi Supriyatno Agoes Mardiono Jacoeb Agus Wijatmoko Agus Yulianto Ahmad Khoirudin Rizkon Aji Hermawan Albarokah, Nurdiansyah Ali Suman Anhar Rozi Arafah, Purnama Ardina, Carissa Mutiara Arivai Santosae, Zacky As Syaffa Amalia Adha Bambang Riyanto Bayu Prasetyo Subekti berutu, fanny indah sari Budy Wiryawan Bustami Ibrahim cholil - anwar Clara M Kusharto Clara Maria Kusharto Clara Meliyanti Kusharto Danang Aria Nugroho Darma Sri Wahyuni Desi Listiana Dian Prima Christiani Hulu Didik Suhardjito Dini Wulan Dari Djoko Poernomo Dudu Abdulatip Eko Sri Wiyono Ema Hastarini Emy Mupid Faleh Setia Budi Fanni Al Fanany Farina Ekarini Fariz Pari, Rizfi Hanani Putri Yocinta Hanifah, Nur Inda Annisa'ul Haryati, Kristina Heryani, Heryani I Wayan Kukuh Feryana Ikeu Tanziha Irfan Gunawan Matondang Iriani Setyaningsih Irzal Effendi isna kurniati afifudin Isti'anah, Ismi Ita Srihastuti Joko Ahadi Priyanto Joko Santoso Joko Santoso Julian Franklin Soselisa Kamini Kamini Kamini Kamini Kamini Kamini, Kamini Kriswantriyono, Agit Kurniawati Kurniawati Kusnendi, Faizal Shofwan Kustiariyah Tarman Kustiariyah, Kustiariyah La Ode Huli Lia Sutiani Listiana, Desi Luh Putu Ratna Sundari MADE ASTAWAN Marhamah, Aulia Shofia Rahmatu MARIA BINTANG Maulana Ishak Mia Srimiati Miftahudin . Mubarokah, Ulfah Muhamad Musbah Muhammad Agung Sedayu N. Nurjanah Nancy Dewi Yuliana Nurjanah - - Nurjanah . Nurjanah Nurjanah Nurjannah, Farah Pipih Suptijah Pipin Supinah Pitria Andriyani Putri Julia Mizulni Putri, Silmiyah RA Hangesti Emi Widyasari Rachman, Sitti Hardiyanti Rakhmawati, Ida Ayu Iska Ramadhan, Muhamad Fauzi Ramadhan, Muhammad Anugerah Ramadhan, Rizki Tri Kurnia Resmi Rumenta Siregar Retno Asih Roni Nugraha Safira Rahma Fauzia Saiful Akhyar Lubis Salimah, Hafshah Aqidatun Sapta Raharja Setiawan, Jeffry Shafira Nurfadhila Sinta Triyanti Siti Rodliyatun Sitti Hardiyanti Rachman Sri Ayu Insani Sri Purwaningsih Stephanie Bija sudianto Sugeng Hari Wisodo Suhaima, Nurafi Razna Sumardjo Supijatno Syamsul Bahri Agus, Syamsul Bahri Syifa Aulia TATI NURHAYATI Tenny - Faradiba Titot Bagus Arifianto Tjondro, Tjondro Tri Wiji Nuraini Vivi Permata Sari Wahyu Ramadhan Widiarsyah, Farel Widodo Widodo Wini - Trilaksani Wulandari, Yulia Puspadewi Yane Regiyana, Yane Yonvitner -