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Reducing Pb accumulation in roots of sweet potato under low lead-contaminated soil by Azotobacter inoculation Reginawanti Hindersah; Vera Oktavia Subarja; Pujawati Suryatmana; Rija Sudirja; Agung Karuniawan; Yusup Hidayat
Journal of Degraded and Mining Lands Management Vol 10, No 2 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2023.102.4271

Abstract

Agricultural soil is possibly threatened by lead (Pb) contamination due to the intensive use of fertilizers. The rhizobacteria were recommended for the bioremediation of soils contaminated by low concentrations of Pb. The experiment was conducted to observe the Azotobacter's ability to proliferate in Pb-contaminated broth and to decrease the Pb availability in soil, Pb uptake by sweet potato roots, and sweet potato growth. The resistance test was performed by growing five Azotobacter isolates in N-free broth with various Pb levels. A pot experiment was conducted in a factorial randomized block design to test three levels of Pb in soil and two Azotobacter isolates. The results showed that Azotobacter Azv4 and A. choroococcum were resistant to 100 mg L-1 Pb in N-free broth. In the pot experiment, Azotobacter Azv4 Inoculation caused less Pb in soil and roots of sweet potatoes grown in Pb-contaminated soil than A. choroococcum. Either Azotobacter or Pb soil did not influence vine length. However, Azv4 was more prominent in increasing branch number, root volume and length; higher Pb in soil reduced branch number but did not affect root parameters. Azotobacter Azv4 increased more shoot and root dry weight compared to A. choroococcum, but both isolates did not change the shoot-to-root ratio (S/R). The Pb contamination only reduced root dry weight and reduced the S/R. This research considered utilizing rhizobacteria Azotobacter for reducing Pb levels in soil and roots; and increasing sweet potato biomass.
PENGARUH PENAMBAHAN TOP SOIL INCEPTISOL DAN KOMPOS PADA TAILING AMALGAMASI TERHADAP PANJANG SULUR, DIAMETER SULUR DAN JUMLAH CABANG TANAMAN UBI JALAR (Ipomoea batatas L.) Indra Herliana; Pujawati Suryatmana; Reginawanti Hindersah; Rhazista Noviardi
Jurnal Tanah dan Sumberdaya Lahan Vol. 8 No. 1 (2021)
Publisher : Departemen Tanah, Fakultas Pertanian, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.965 KB) | DOI: 10.21776/ub.jtsl.2021.008.1.19

Abstract

Tailings are waste generated from the processing of gold ore. The tailings in Kertajaya Village, Sukabumi are generally dumped into a pond or garden which is then used by the community to grow crops. The characteristics of the tailings, which contain low organic matter, microorganism activity, CEC and essential nutrients, make it necessary to improve their condition. This study aimed to determine the effect of adding top soil and compost to the tailings on the growth of sweet potatoes in the vegetative phase. This experiment used a factorial randomized block design with two factors, namely a top soil-tailing ratio of 3 levels (70: 30% w/w; 50: 50% w/w; and 30: 70% w/w) and a compost dose of 4. level (without compost (control); 10 t ha-1; 20 t ha-1; and 30 t ha-1) with 3 replications. The results showed that the combination of the addition of top soil ratios and the dose of compost to the tailings had no effect on the addition of tendrils length, tendril diameter and number of sweet potato branches during the vegetative phase. The addition of 70% top soil independently on the tailings had an effect on the highest increase in tendril length, tendril diameter and number of branches of sweet potato. Meanwhile, the application of compost dosage of 30 t ha-1 had the highest effect on the number of branches   of sweet potato.
Azotobacter and Pseudomonas Population in Rhizosphere and Yield of Choy Sum following Chemical Fertilizer and Biofertilizer application Reginawanti Hindersah; Nini Mila Rahmi
Agrologia Vol 9, No 2 (2020): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajibt.v9i2.1161

Abstract

The limestone hilly landscapes, well  known as karst hills, in Muna Island resulted in natural grow of cashew (Anacardium occidentale L.). The karst area alos  support the cultivation of local food crops such as maize. Both local products need to be maintained in order to increase the community's revenue and give the identity to Muna Island as cashew nuts and maize producer. Cashew and maize are the leading commodities of Muna Island. This article describes the cultivation conditions and economic value of cashew nuts and maize in Muna Island. However, to achieve optimal production and selling value, technology adoption either in the field or  post-harvest, needs to be improved especially for cashew.
Effect of Molybdenum on Population of Bacillus Consortium and Production of Indole 3-Acetic Acid Aliya Zahrah Adawiah; Sandra Amalia Riyadi; Reginawanti Hindersah; Pujawati Suryatmana
Agrologia Vol 12, No 1 (2023): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajibt.v12i1.1771

Abstract

Molybdenum (Mo) is one of nutrients that contributes on process of fixing N2 and it’s contained in growth medium of N2-fixing bacteria. Bacillus is bacterium that widely used as biofertilizer because it can fix N2 in the atmosphere and produce phytohormones in form of Indole 3-Acetic Acid (IAA) which are benefical for plant growth. The purpose of this research was to determine the effect of Mo addition on Bacillus consortium in a growth medium of 2%molasses + 10grams peptone + 3grams beef extract against vegetative cell population, spore population and IAA hormone production after incubated for 3, 6 and 9 days. This research was conducted at Soil Biology Laboratory, Faculty of Agriculture, Padjadjaran University. The method to calculate total population of vegetative cells is the serial dilution plate method with 80ºC heating for 30 minutes for total spore population, while the production of IAA hormone was measured using UV-Vis spectrophotometer with Salkowski reagent at a wavelength of 530 nm. All data subjected to Student-t test with p ≤ 0.05. The results of the study by using the Student-t test showed that Mo increased the vegetative cell population on day 3 but did not affect the vegetative cell population on day 6 and 9. The spore population of the Bacillus consortium was not determined by Mo. The production of IAA hormone in cultures with Mo was not different from that in cultures without Mo.
The effect of beneficial microorganism as biofertilizer application in hydroponic-grown tomato Mieke Rochimi Setiawati; Nandha Afrilandha; Reginawanti Hindersah; Pujawati Suryatmana; Betty Natalie Fitriatin; Nadia Nuraniya Kamaluddin
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 20, No 1 (2023): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v20i1.63877

Abstract

The use of N2-fixing bacteria and growth hormone-producing rhizobacteria delivers nitrogen, enhances nutrients absorption by plants, and reduces the usage of inorganic fertilizers. Implementing biofertilizer in the hydroponic system as a means to reduce application of synthetic nutrient is recently in interest due to economic, food safety, and sustainability factors. This study determines the effects of biofertilizer dose on tomato yields in the hydroponics system. A randomized block design was utilized that consisted of seven treatments, namely 100% inorganic fertilizer and 0% biofertilizer (control), and various doses of inorganic nutrient combined with 25%, 50%, 75%, and 100% biofertilizer. The result illustrated that the application of biofertilizer augmented the population of endophytic bacteria, Azotobacter sp., Azospirillum sp., phosphate solubilizing bacteria, and nitrogen content. The distinct combination of biofertilizer did not alter the phosphorus and potassium content compared to control samples however resulted in superior tomato grade. The 50% inorganic fertilizer and 100% biofertilizer combination amplified the weight of the fruit by 36% compared to the control. This finding indicates that the application of biofertilizers in the hydroponic system for tomato plants is not only beneficial in minimizing the dosage of inorganic fertilizers but also enhancing the fruit quality.
Selection and Bioassay of Azotobacter sp. Isolates to Improve Growth of Chili (Capsicum annum L.) on Entisols in Ambon REGINAWANTI HINDERSAH; PRIYANKA PRIYANKA; WILHELMINA RUMAHLEWANG; A MARTHIN KALAY
Microbiology Indonesia Vol. 10 No. 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (677.784 KB) | DOI: 10.5454/mi.10.4.2

Abstract

Leafy vegetables contributes to the inflation rate in Ambon City due to low productivity in rainy season. Some vegetables are imported from other islands while importantvegetables such as local petsai (Brassica chinensis L.) and chili (Capsicum annum L.) are cultivated in low nitrogen soil, Entisols. Lack of nitrogen could be overcome by using inorganic fertilizeras well as biofertilzer. The soil can be inoculated with rhizobacteria, such as Azotobacter, to increase  the nitrogen uptake and improve the quality of vegetables. This research was conducted to isolate and select Azotobacter from rhizosphere of vegetables and to examine the effect of Azotobacter inoculation on chili-seedling growth and nitrogen uptake by using bioassay method. Azotobacter sp. was isolated in nitrogen-free Ashby’s Media. The bioassay was held in the green house with randomized block design experiment, which examined the combination of isolates and population of Azotobacter sp. on chili. Two best isolates which was selected based on pH, nitrogen content and cell viability were s2a10 (from petsai's rhizosphere) and c2a9 (from chili’s rhizosphere). Bioassay showed that Azotobacter inoculation followed by reduced NPK fertilizer doses had no effect on transplant dry weight and nitrogen uptake. All Azotobacter 8 -1inoculation except  10 CFU mL s2a10 maintain soil nitrogen although Azotobacter population in soil was slightly reduced. This showed that Azotobacter sp. potentially reduce the use of inorganic biofertilizer.
Humic Acid for Reducing Carbofuran Content of Rice Grown in Carbofuran-Contaminated Soil Reginawanti Hindersah; Betty Natalie Fitriatin; Triyani Dewi; Dedi Nursyamsi
Jurnal Agroekoteknologi Vol 15, No 1 (2023)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v15i1.20049

Abstract

Carbofuran insecticide widely used in rice production. The carbofuran residue in agricultural threatens wide life and human health since its active ingredient carbamate is toxic. Humic acid (HA) amendment is an effective way to minimize the residue in soil since HA can adsorb the organic pollutant and induce growth of fungus that degrade the carbofuran. The objective of this research was to observe the influence of HA on the carbofuran residues in soil, straw, and rice; as well as yield of paddy (Oryza sativa L.) grown in carbofuran contaminated potted soil. The level of carbofuran residue in soil before experiment was 10 mg kg-1. The experiment was setup in Randomized Block Design to test various HAs extracted from organic matter. The results indicated that humic acid didn’t affect plant growth and yield but decreased the carbofuran residue in straw and unhusked rice grain. Rhizosphere of rice treated with HA showed higher fungal count than control. This experiment suggests that HA were effectively used to control the carbofuran residue in soil and rice grown in low carbofuran-contamination agricultural soil
PERAN AGEN HAYATI AZOTOBACTER-TRICHODERMA TERHADAP PERTUMBUHAN DAN HASIL CABAI MERAH (Capsicum annuum L.) PADA PERCOBAAN POT Reginawanti Hindersah; Priyanka Asmiran; June Putinella; Wilhelmina Rumahlewang; Marthin Kalay
Agric Vol. 29 No. 2 (2017)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2017.v29.i2.p137-146

Abstract

Mikroba tanah dapat digunakan sebagai input pertanian. Rizobakteri Azotobacter pemfiksasi nitrogen menjaga ketersediaan unsur hara nitrogen dan pertumbuhan tanaman sedangkan Trichoderma mampu menurunkan kejadian penyakit tular tanah melalui aktivitas antagonistik. Percobaan pot ini bertujuan untuk mendapatkan dosis dan waktu aplikasi inokulan Azotobacter- Trichoderma yang dapat meningkatkan ketersediaan nitrogen tanah, pertumbuhan dan hasil tanaman cabai (Capsicum annuum L.). Bibit cabai ditanam di tanah dengan kesuburan rendah yang dicampur dengan pupuk kotoran sapi. Rancangan percobaan adalah rancangan petak terbagai yang menguji empat taraf dosis inokulan dan tiga waktu aplikasi. Hasil percobaan memperlihatkan bahwa tidak ada pengaruh agen biologi terhadap tinggi tanaman pada tiga dan enam minggu setelah tanam. Inokulasi 7,5 g pot-1 inokulan padat Azoto-Tricho pada saat tanam secara signifikan meningkatkan kadar NO3 - tanah jika diikuti dengan penyiraman inokulan cair Azotobacter ke tanah pada 10 hari setelah tanam. Inokulan padat Azoto-Tricho meningkatkan hasil tanaman. Hasil tertinggi, 290 g per tanaman diperoleh dari tanaman yang diberi 7,5 g Azoto-Tricho pada saat tanam diikuti aplikasi inokulan cair Azotobacter. Percobaan pot ini menjelaskan bahwa inokulan padat Azotobacter-Trichoderma berpotensi untuk digunakan sebagai agen hayati pada produksi cabai.
NITROGEN FIXING BACTERIA AZOTOBACTER AS BIOFERTILIZER AND BIOCONTROL IN LONG BEAN Reginawanti Hindersah; Marthin Kalay; Abraham Talahaturuson; Yansen Lakburlawal
Agric Vol. 30 No. 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2018.v30.i1.p25-32

Abstract

Azotobacter is Plant Growth Promoting Rhizobacteria through the mechanism of nitrogen fixation and phytohormon production but this rhizobacteria has a role to control plant diseases. The objective of experiment was to evaluate the activity of Azotobacter as biofertilizers as well as biocontrol on long bean cultivation in damping off endemic land in Ambon city, Maluku Province. The field experiment was arranged in completely randomized block design. Inoculation of long bean by Azotobacter has been done by seed inoculation, soil inoculation before planting, and plant inoculation. Plants treated with Azotobacter received fertilizer NPK of ¾ or ½ dosage recommendation while control plants were received 100% NPK. Research showed that no differences between yield of long bean inoculated with Azotobacter sp +reduced doses of NPK with that of control plants. Any application method of Azotobacter inoculation lowered damping off diseases incidence significantly until 10 days after planting, but no effect of inoculation on late blight at 21 days after planting. This study confirmed that Azotobacter has dual activity to reduce the dose of NPK fertilizer and control damping off.
Pengaruh Metode Aplikasi Azotobacter terhadap Hasil Kedelai (Glycine max (L.) Merill) di Lahan Kering Reginawanti Hindersah; Rara Rahmantika Risanti; Ibnu Haikal; Yuliati Mahfud; Nenny Nurlaeny; Meddy Rachmadi
Agric Vol. 31 No. 2 (2019)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2019.v31.i2.p136-145

Abstract

Pupuk hayati dengan bahan aktif rizobakteri Azotobacter mampu meningkatkan kesuburan tanah dan meningkatkan pertumbuhan tanaman melalui fiksasi nitrogen dan produksi fitohormon. Tujuan penelitian ini adalah membandingkan respons tanaman serkedelai (Glycine max (L.) Merill) di lahan kering setelah aplikasi beberapa metode inokulasi Azotobacter. Percobaan lapangan dilakukan dengan Rancangan Acak Kelompok yang terdiri atas lima perlakuan dan lima ulangan. Perlakuan yang diuji adalah inokulasi benih, inokulasi melalui tanah sebelum tanam, inokulasi melalui tanah setelah tanam dan inokulasi melalui tajuk tanaman. Pertamanan dengan inokulasi Azotobacter diberi pupuk urea 50 % dari dosis rekomendasi sedangkan tanaman kontrol mendapatkan urea dosis rekomendasi. Hasil percobaan menunjukkan bahwa seluruh metode aplikasi tidak mempengaruhi produksi kedelai, jumlah nodula, populasi Azotobacter di rizosfer dan N total tanah; tetapi aplikasi Azotobacter sp melalui daun meningkatan serapan N dan bobot 100 biji kedelai.
Co-Authors A.M. Kalay Abraham Talahaturuson, Abraham Adawiah, Aliya Zahrah Adawiyah, Aliya Z Ade Setiawan Ade Setiawan Adita Rizky Syntianis Aditya, Fasa Aditya, Fasa Aditya, Fasa Agnia Nabila Agung Karuniawan Agung Muhammad Yusuf Agus Jacob Ai Siti Santriyani Aisyah, Ayu Siti Akustu, Masako Alia Halimatusy Aliya Zahrah Adawiah Alyani Shabrina amiruddin teapon Ane Nurbaity Ani Yuniarti Anne Nurbaity ANNE NURBAITY Anni Yuniarti Anny Yuniarti Antonio Yusuf Ariani, Nabila Syifa Arief Harsono Asep Purnama Hidayat Ashwath, Nanjappa Asmiran, Priyanka Aten Komarya Bagus Adityo Bang, Sunbaek Bari, Ichsan Nurul Benito R. Kurnani Benny Joy Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Chotimah, Andina D Herdiyantoro D. A Sulaksana Dadang Epi Sukarsa Damayani, Maya Dedeh H. Arief Dedeh Hudaya Arief Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedy Nursyamsi Dewi, Yeni Wispa Dewikusuma Ikhsani Dewikusuma Ikhsani Adhiningtyas Diana Nafitri Cahayaningrum Diky Indra Wibawa Dirga Sapta Sara Diyah Sri Utami DIYAN HERDIYANTORO Diyan Herdiyantoro Diyan Herdiyantoro Dwi Suci Rahayu ELENA F. L. LILIPALY Endo, Kenji Erni Suminar Erwin Erwin Etty Pratiwi Fauzul Amri Febby Nur Indriani Febby Nur Indriani Fera Siti Meilani Ferra Langoi Fiky Yulianto Wicaksono Fitriatin, Betty Fitriatin, Betty N Fitriatin, Betty Natalie Gina Nurhabibah H Yulina Handayani, Sri Haris Maulana Harry Rum Haryadi Harsono, dan Arief Henry Kesaulya Herman Rehatta Hidiyah Ayu Ratna Ma’rufah Ibnu Haikal Iis Nur Asyiah Imam Mudakir Imran Makatita Indra Herliana Irene A. Ngabalin Ishak, Lily Jajang Sauman Hamdani James Matheus June A Putinella June Putinella Juniart Leklioy Kaffah, Ruhnayati Latifah, Tipah Lukman Gunarto M Riadh Uluputty Maharani, Nadhira Saniya Mahdi Argawan Putra Mahfud Arifin Mardiyani Sidayat Marina Jamlean Martha Fani Cahyandito Matsuura, Asana Maulana, Nurzen Mauludy, Noor Muhammad Maya Damayani Mayang Agustina Meddy Rachmadi Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mudiyati Rahmatunnisa Mulyadi Mulyadi Nadia Nuraniya Kamaluddin Nakayama, Masakazu Nandha Afrilandha NANJAPPA ASHWATH Neni Rostini NENNY NURLAENY Nenny Nurlaeny Nia Kurniati Nia Kurniati Nia Kurniati Ninda Meiditia Putri Nini Mila Rahmi Nizar Ulfah Nono Carsono Nugraha, Gita Bina Nugraha, Gita Bina Nugraha, Gita Bina Nurfitriana, Nofalia Nurmayulis Nuryani, dan Oktaviani, Meira Osok, Rafael M Oviyanti Mulyani Priyanka Asmiran Priyanka Asmiran PRIYANKA PRIYANKA Probo Condrosari Pudjawati Suryatmana Pujawati Suryatmana Puji Syara Anggia Putri Sri Judiani Purba Rachelita, Nadia Rachmat Harryanto Rara Rahmantika Risanti Rhasajati, Dhia Rhazista Noviardi Rhazista Noviardi Riadh Uluputty Rija Sudirja Rina Devnita Risanti, Rara Risanti, Rara Rahmatika Risanti, Rara Rahmatika Rita Harni Rita Harni Robby Risamasu Robi Risamasu Rudi Priyadi Rustam Rustam Salsabilla, Camilla Sandra Amalia Riyadi Sandrawati, Apong Saon Banerjee Sarita Sarkar Setiawati, Mieke Setiawati, Mieke Richimi Setiawati, Mieke Rochimi Shabrina Rahma Fauzia Soetijoso Soemitro Solihin, Amir Sondi Kuswaryan Suman Samanta Sumbada, Rifyal Achmad Rivaldi Sunardi Sunardi Sunarya, Yaya - Tarkus Suganda Toto Sunarto Tri Hanggono Achmad Tri Mulya Hartati Triani Dewi Triani Dewi Triyani Dewi Triyani Dewi Triyani Dewi Tualar Simarmata Vera Oktavia Subarja Vira Kusuma Dewi Wahyuda Rachman, Wahyuda Wawan Kurniawan Wawan Rachman Wilhelmina Rumahlewang Wilhelmina Rumahlewang Wilhemina Rumahlewang Yansen Lakburlawal Yuliati Mahfud Yusi Firmansyah Yusuf Rahman Yusup Hidayat Zellya Handyman