Articles
Azotobacter and Pseudomonas Population in Rhizosphere and Yield of Choy Sum following Chemical Fertilizer and Biofertilizer application
Reginawanti Hindersah;
Nini Mila Rahmi
Agrologia Vol 9, No 2 (2020): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University
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DOI: 10.30598/ajibt.v9i2.1161
The limestone hilly landscapes, well known as karst hills, in Muna Island resulted in natural grow of cashew (Anacardium occidentale L.). The karst area alos support the cultivation of local food crops such as maize. Both local products need to be maintained in order to increase the community's revenue and give the identity to Muna Island as cashew nuts and maize producer. Cashew and maize are the leading commodities of Muna Island. This article describes the cultivation conditions and economic value of cashew nuts and maize in Muna Island. However, to achieve optimal production and selling value, technology adoption either in the field or post-harvest, needs to be improved especially for cashew.
Azotobacter and Pseudomonas Population in Rhizosphere and Yield of Choy Sum following Chemical Fertilizer and Biofertilizer application
Reginawanti Hindersah;
Adita Rizky Syntianis;
Ade Setiawan;
Rina Devnita;
Nursuhud Nursuhud
Agrologia Vol 12, No 1 (2023): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University
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DOI: 10.30598/ajibt.v12i1.1774
Inoculation of beneficial microbes is a way to improve the population of rhizobacteria that has a role in nutrient cycle and hence reduce the level of chemical fertilizer. A pot experiment was performed to evaluate the effect of and consortia biofertilizer combined with various dose of NPK compound fertilizer on the population of nitrogen-fixing Azotobacter sp. and phosphate solubilizing Pseudomonas sp. in rhizosphere and yield of choy sum (Brassica juncea L.). The experimental was setup in completely randomized block design consisted of ten combinations of various biofertilizer and inorganic fertilizer dose; and three replications. The results showed that Biofertilizer inoculation with or without chemical fertilizer did not improve the population of Azotobacter and Pseudomonas in the rhizosphere but increase the shoot weight of choy sum. Higher shoot weight was showed by crops with ¾ dose of NPK combined with recommended or reduced dose of fertilizer; and recommended dose of NPK and biofertilizer. This experiment verified that the used of consortia biofertilizer composed of Nitrogen-fixing bacteria and phosphate-solubilizing microbes reduced 50-75% dose of NPK.
Effect of Molybdenum on Population of Bacillus Consortium and Production of Indole 3-Acetic Acid
Aliya Zahrah Adawiah;
Sandra Amalia Riyadi;
Reginawanti Hindersah;
Pujawati Suryatmana
Agrologia Vol 12, No 1 (2023): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University
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DOI: 10.30598/ajibt.v12i1.1771
Molybdenum (Mo) is one of nutrients that contributes on process of fixing N2 and it’s contained in growth medium of N2-fixing bacteria. Bacillus is bacterium that widely used as biofertilizer because it can fix N2 in the atmosphere and produce phytohormones in form of Indole 3-Acetic Acid (IAA) which are benefical for plant growth. The purpose of this research was to determine the effect of Mo addition on Bacillus consortium in a growth medium of 2%molasses + 10grams peptone + 3grams beef extract against vegetative cell population, spore population and IAA hormone production after incubated for 3, 6 and 9 days. This research was conducted at Soil Biology Laboratory, Faculty of Agriculture, Padjadjaran University. The method to calculate total population of vegetative cells is the serial dilution plate method with 80ºC heating for 30 minutes for total spore population, while the production of IAA hormone was measured using UV-Vis spectrophotometer with Salkowski reagent at a wavelength of 530 nm. All data subjected to Student-t test with p ≤ 0.05. The results of the study by using the Student-t test showed that Mo increased the vegetative cell population on day 3 but did not affect the vegetative cell population on day 6 and 9. The spore population of the Bacillus consortium was not determined by Mo. The production of IAA hormone in cultures with Mo was not different from that in cultures without Mo.
Selection and Bioassay of Azotobacter sp. Isolates to Improve Growth of Chili (Capsicum annum L.) on Entisols in Ambon
REGINAWANTI HINDERSAH;
PRIYANKA PRIYANKA;
WILHELMINA RUMAHLEWANG;
A MARTHIN KALAY
Microbiology Indonesia Vol. 10 No. 4 (2016): December 2016
Publisher : Indonesian Society for microbiology
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DOI: 10.5454/mi.10.4.2
Leafy vegetables contributes to the inflation rate in Ambon City due to low productivity in rainy season. Some vegetables are imported from other islands while importantvegetables such as local petsai (Brassica chinensis L.) and chili (Capsicum annum L.) are cultivated in low nitrogen soil, Entisols. Lack of nitrogen could be overcome by using inorganic fertilizeras well as biofertilzer. The soil can be inoculated with rhizobacteria, such as Azotobacter, to increase  the nitrogen uptake and improve the quality of vegetables. This research was conducted to isolate and select Azotobacter from rhizosphere of vegetables and to examine the effect of Azotobacter inoculation on chili-seedling growth and nitrogen uptake by using bioassay method. Azotobacter sp. was isolated in nitrogen-free Ashby’s Media. The bioassay was held in the green house with randomized block design experiment, which examined the combination of isolates and population of Azotobacter sp. on chili. Two best isolates which was selected based on pH, nitrogen content and cell viability were s2a10 (from petsai's rhizosphere) and c2a9 (from chili’s rhizosphere). Bioassay showed that Azotobacter inoculation followed by reduced NPK fertilizer doses had no effect on transplant dry weight and nitrogen uptake. All Azotobacter 8 -1inoculation except  10 CFU mL s2a10 maintain soil nitrogen although Azotobacter population in soil was slightly reduced. This showed that Azotobacter sp. potentially reduce the use of inorganic biofertilizer.
The potential of legume cover crops and soil microbes for gold mine tailings revegetation
Triyani Dewi;
Sukarjo Sukarjo;
C O Handayani;
Reginawanti Hindersah;
Edwen D Waas;
A M Kalay;
Hidayatuz Zu’amah
Journal of Degraded and Mining Lands Management Vol 10, No 4 (2023)
Publisher : Brawijaya University
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DOI: 10.15243/jdmlm.2023.104.4593
Mercury (Hg) is commonly utilized in artisanal gold mining on Buru Island; the Hg-contaminated tailing possibly contaminates the agricultural land nearby. In general, tailings contain very low organic carbon and plant nutrients but are high in mercury and have extreme soil acidity. The objective of this study was to observe the growth of various legume cover crops (LCC) and the change of Hg in tailing inoculated with Azotobacter-Trichoderma. The field trial was conducted on Buru Island of Maluku by using a split-plot design with three replications. The main plot was LCC species, composed of Centrosema pubescens (CP), Mucuna sp. (MC), and Crotalaria sp. (CR); the subplots were microbial inoculants composed of two formulations of Azotobacter-Trochoderma inoculants. The results showed that the Mucuna sp. and consortia Azotobacter-Trichoderma (2:1) had the highest survival rate in the tailings and Hg uptake by 8.83 mg kg-1 per plant. Consortia Azotobacter-Trichoderma inoculant with the composition of 2:1 (v:v) was able to increase soil pH, total bacterial population, LCC biomass, and Hg uptake by LCC plants. The highest Hg removal effectivity was observed in the plot treated with Crotalaria sp. and in a plot with Azotobacter-Trichoderma (2:1), which was 34.0% and 33.6%, respectively.
Humic Acid for Reducing Carbofuran Content of Rice Grown in Carbofuran-Contaminated Soil
Reginawanti Hindersah;
Betty Natalie Fitriatin;
Triyani Dewi;
Dedi Nursyamsi
Jurnal Agroekoteknologi Vol 15, No 1 (2023)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta
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DOI: 10.33512/jur.agroekotetek.v15i1.20049
Carbofuran insecticide widely used in rice production. The carbofuran residue in agricultural threatens wide life and human health since its active ingredient carbamate is toxic. Humic acid (HA) amendment is an effective way to minimize the residue in soil since HA can adsorb the organic pollutant and induce growth of fungus that degrade the carbofuran. The objective of this research was to observe the influence of HA on the carbofuran residues in soil, straw, and rice; as well as yield of paddy (Oryza sativa L.) grown in carbofuran contaminated potted soil. The level of carbofuran residue in soil before experiment was 10 mg kg-1. The experiment was setup in Randomized Block Design to test various HAs extracted from organic matter. The results indicated that humic acid didn’t affect plant growth and yield but decreased the carbofuran residue in straw and unhusked rice grain. Rhizosphere of rice treated with HA showed higher fungal count than control. This experiment suggests that HA were effectively used to control the carbofuran residue in soil and rice grown in low carbofuran-contamination agricultural soil
Abundance of Beneficial Soil Microbes in the Rhiosphere of Coffee Plants Infected by Pratylenchus coffeae
Reginawanti Hindersah;
Iis Nur Asyiah
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 2 (2023): August 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro
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DOI: 10.15294/biosaintifika.v15i2.43085
Enzymatic transformation in the cycle of nutrients of Nitrogen (N) and phosphorus (P) in soil can only be carried out by microbes. The latest approach in coffee cultivation is to utilize soil microbes to enhance plant growth, also to reduce the attack of the nematode Pratylenchus coffeae on coffee roots. This exploratory study aimed to observe the attack of P. coffeae and the presence of N-fixing bacteria (NFB) and P- solubilizing microbes (PSM) in the coffee rhizosphere which in turn have the potential to be used as biological fertilizers and bioprotectants of coffee plants. The study was conducted in PT Perkebunan Kalibendo's Arabica and Robusta coffee fields, East Java, on immature plants (IP) and mature plants (MP). This exploration explained that the IP and MP arabica and robusta coffee plantations were attacked by P. coffeae. NFB and PSM were successfully isolated from the rhizosphere of both coffee plantations types. The population of NFB Azotobacter in IP was lower than in MP, but the PSM population in IP rhizosphere was not different from that in MP. Based on morphological and biochemical characterization, three isolates of Azotobacter bacteria, six species of P- solubilizing bacteria (PSB) and six species of phosphate-solubilizing fungi (PSF) were obtained. This exploration confirmed that the microbes involved in the N and P cycle colonized the coffee rhizosphere which was attacked by P. coffeae. Further research is recommended to observe the effectiveness of microbes as biological fertilizers and bioprotectants for coffee plants.
PERAN AGEN HAYATI AZOTOBACTER-TRICHODERMA TERHADAP PERTUMBUHAN DAN HASIL CABAI MERAH (Capsicum annuum L.) PADA PERCOBAAN POT
Reginawanti Hindersah;
Priyanka Asmiran;
June Putinella;
Wilhelmina Rumahlewang;
Marthin Kalay
Agric Vol. 29 No. 2 (2017)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana
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DOI: 10.24246/agric.2017.v29.i2.p137-146
Mikroba tanah dapat digunakan sebagai input pertanian. Rizobakteri Azotobacter pemfiksasi nitrogen menjaga ketersediaan unsur hara nitrogen dan pertumbuhan tanaman sedangkan Trichoderma mampu menurunkan kejadian penyakit tular tanah melalui aktivitas antagonistik. Percobaan pot ini bertujuan untuk mendapatkan dosis dan waktu aplikasi inokulan Azotobacter- Trichoderma yang dapat meningkatkan ketersediaan nitrogen tanah, pertumbuhan dan hasil tanaman cabai (Capsicum annuum L.). Bibit cabai ditanam di tanah dengan kesuburan rendah yang dicampur dengan pupuk kotoran sapi. Rancangan percobaan adalah rancangan petak terbagai yang menguji empat taraf dosis inokulan dan tiga waktu aplikasi. Hasil percobaan memperlihatkan bahwa tidak ada pengaruh agen biologi terhadap tinggi tanaman pada tiga dan enam minggu setelah tanam. Inokulasi 7,5 g pot-1 inokulan padat Azoto-Tricho pada saat tanam secara signifikan meningkatkan kadar NO3 - tanah jika diikuti dengan penyiraman inokulan cair Azotobacter ke tanah pada 10 hari setelah tanam. Inokulan padat Azoto-Tricho meningkatkan hasil tanaman. Hasil tertinggi, 290 g per tanaman diperoleh dari tanaman yang diberi 7,5 g Azoto-Tricho pada saat tanam diikuti aplikasi inokulan cair Azotobacter. Percobaan pot ini menjelaskan bahwa inokulan padat Azotobacter-Trichoderma berpotensi untuk digunakan sebagai agen hayati pada produksi cabai.
NITROGEN FIXING BACTERIA AZOTOBACTER AS BIOFERTILIZER AND BIOCONTROL IN LONG BEAN
Reginawanti Hindersah;
Marthin Kalay;
Abraham Talahaturuson;
Yansen Lakburlawal
Agric Vol. 30 No. 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana
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DOI: 10.24246/agric.2018.v30.i1.p25-32
Azotobacter is Plant Growth Promoting Rhizobacteria through the mechanism of nitrogen fixation and phytohormon production but this rhizobacteria has a role to control plant diseases. The objective of experiment was to evaluate the activity of Azotobacter as biofertilizers as well as biocontrol on long bean cultivation in damping off endemic land in Ambon city, Maluku Province. The field experiment was arranged in completely randomized block design. Inoculation of long bean by Azotobacter has been done by seed inoculation, soil inoculation before planting, and plant inoculation. Plants treated with Azotobacter received fertilizer NPK of ¾ or ½ dosage recommendation while control plants were received 100% NPK. Research showed that no differences between yield of long bean inoculated with Azotobacter sp +reduced doses of NPK with that of control plants. Any application method of Azotobacter inoculation lowered damping off diseases incidence significantly until 10 days after planting, but no effect of inoculation on late blight at 21 days after planting. This study confirmed that Azotobacter has dual activity to reduce the dose of NPK fertilizer and control damping off.
Pengaruh Metode Aplikasi Azotobacter terhadap Hasil Kedelai (Glycine max (L.) Merill) di Lahan Kering
Reginawanti Hindersah;
Rara Rahmantika Risanti;
Ibnu Haikal;
Yuliati Mahfud;
Nenny Nurlaeny;
Meddy Rachmadi
Agric Vol. 31 No. 2 (2019)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana
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DOI: 10.24246/agric.2019.v31.i2.p136-145
Pupuk hayati dengan bahan aktif rizobakteri Azotobacter mampu meningkatkan kesuburan tanah dan meningkatkan pertumbuhan tanaman melalui fiksasi nitrogen dan produksi fitohormon. Tujuan penelitian ini adalah membandingkan respons tanaman serkedelai (Glycine max (L.) Merill) di lahan kering setelah aplikasi beberapa metode inokulasi Azotobacter. Percobaan lapangan dilakukan dengan Rancangan Acak Kelompok yang terdiri atas lima perlakuan dan lima ulangan. Perlakuan yang diuji adalah inokulasi benih, inokulasi melalui tanah sebelum tanam, inokulasi melalui tanah setelah tanam dan inokulasi melalui tajuk tanaman. Pertamanan dengan inokulasi Azotobacter diberi pupuk urea 50 % dari dosis rekomendasi sedangkan tanaman kontrol mendapatkan urea dosis rekomendasi. Hasil percobaan menunjukkan bahwa seluruh metode aplikasi tidak mempengaruhi produksi kedelai, jumlah nodula, populasi Azotobacter di rizosfer dan N total tanah; tetapi aplikasi Azotobacter sp melalui daun meningkatan serapan N dan bobot 100 biji kedelai.