Claim Missing Document
Check
Articles

Identification of nifD and nifH Genes of Methanotrophic Bacteria from Rice Field Ari Fina Bintarti; Iman Rusmana; Aris Tri Wahyudi
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (879.425 KB) | DOI: 10.14203/ann.bogor.2014.v18.n2.13-25

Abstract

Metanotrophic bacteria have ability to oxidize methane and fix atmospheric nitrogen, hence the bacteria has an important role as a nitrogen source provider on wetland area like rice fields. Nitrogen fixation process is catalyzed by the nitrogenase enzyme complex, encoded by nifD and nifH genes. However, characteristic of these genes from indigenous-methanotrophic bacteria still poorly understood. Hence, nifD and nifH genes of methanotrophic bacteria isolated from rice fields in Indonesia (BGM3, BGM9, SS1, SS3, SS10, ST18, SP3 and INP4) were identified and characterized. Detection of nifH and nifD genes was conducted by polymerase chain reaction (PCR) amplification. nifH and nifD gene sequences were analyzed using BLAST-X and phylogenetic trees were constructed using Neighbour Joining method. Based on nifH sequences analysis, SS1 closely related to Beijerinckia mobilis and SS3, SS10, ST 18 closely related to Beijerinckia indica subsp. indica ATCC 9039, while, BGM3, INP4, and BGM9 related to nifH of uncultured nitrogen-fixing bacterium. In other hand, sequence analysis of nifD gene showed that SS1, SS3, SS10, ST 18 closely related to B. indica subsp. indica ATCC 9039 and BGM3, BGM9, INP4 closely related to Xanthobacter autotrophicus Py2. Identification by 16 SrRNA indicated that SS1, SS3, SS10, and ST18 had closeness to Beijerinckia sp. P310-1, while INP4 closely related to Xanthobacter sp. M5C24.
Metode Pengukuran dan Model Pendugaan Biomassa Nypa Fruticans di Sungai Tallo, Makassar-Indonesia Rahman Rahman; Hefni Effendi; Iman Rusmana; mukti ali
Grouper: Fisheries Scientific Journal Vol 11, No 1 (2020): Grouper : Jurnal Ilmiah Fakultas Perikanan Universitas Islam Lamongan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v11i1.65

Abstract

Penelitian tentang estimasi dan model pendugaan biomassa pada tegakan pohon mangrove telah banyak dilakukan baik dalam tingkat ekosistem maupun spesies, namun belum pernah dilakukan pada spesies Nypa fruticans. Penelitian ini bertujuan untuk membuat sebuah model penduga nlilai biomassa Nypa fruticans di Sungai Tallo yang terdapat di Makassar-Indonesia yang merupakan salah satu habitat mangrove. Pendugaan biomassa dilakukan dengan menebang mangrove sebanyak 15 pohon pada range diameter terbesar hingga terkecil. Biomassa dihitung berdasarkan berat kering yang diperoleh dari pengeringan sub contoh berat basah pada suhu 130 ⁰C selama 48 jam. Analisis model penduga biomassa menggunakan regresi linier dan model allometrik yang akan menyatakan hubungan antara diameter pangkal atau pelepah dengan nilai biomassa mangrove. Hasil analisis menunjukkan bahwa model penduga biomassa Nypa fruticansbersifat allometrik yang berkorelasi dengan diameter pangkal dan diameter pelepah yaitu B = 0,098(DB)1,4934  dan B = 0,222(DS)2,7048 dan lebih baik dibandingkan model analisis regresi. Total biomassa Nypa fruticans menurut diameter pangkal dan pelepah yakni 174574,4kgatau 40132, 69 kg/ha dan 173959,4kg atau 39991, 32 kg/ha.
Analysis of Soil Bacterial Diversity from Tropical Rainforest and Oil Palm Plantation In Jambi, Indonesia by 16S rRNA-DGGE Profiles Risky Hadi wibowo; Nisa Rachmania Mubarik; Iman Rusmana; Maggy Thenawidjaya Suhartono; Sipriyadi Sipriyadi; Masrukhin Masrukhin
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.68820

Abstract

Oil palm plantations are the most invasive land use changes in Southeast Asia. It must have affected unique natural biodiversity. This study aimed to investigate the diversity of soil bacteria based on 16S rRNA gene profiles from tropical forest and oil palm plantation in Jambi Province, Indonesia. Soil sample was taken from tropical forest and oil palm plantation from Jambi province, Indonesia. The forest site is in Bukit Duabelas National Park, and the nearby oil palm plantation is in Sarolangun District, Jambi Province, Indonesia. The diversity of bacterial communities from topsoil was studied using Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene and common biodiversity indices. PCR amplification of 16S rRNA gene was successfully conducted primers-using 33F/ 518R primers. Phylogenetic approach was used for revealing the community shift of bacterial phyla and genera in both areas. Phylogenetic analysis showed there were 4 phyla of bacteria i.e., Firmicutes, Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria, respectively. Actinobacteria was the most dominant group in both areas. The composition of soil bacterial community in the oil palm plantation, based on total number of bands 16S rRNA generated from DGGE was richer than that in the Bukit Dua Belas National Park. It was probably caused by plantation year circle more than 10 years and routine activities during the plantation management, such as applications of agricultural lime, herbicide and fertilizer. 
The Abundance of Nitrogen Fixing, Nitrifying, Denitrifying and Ammonifying Bacteria in the Soil of Tropical Rainforests and Oil Palm Plantations in Jambi Zulfarina,; Rusmana, Iman; Mubarik, Nisa Rachmania; Santosa, Dwi Andreas
Makara Journal of Science Vol. 21, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Alterations in the use of land for oil palm plantations can change the domination and activity of soil bacteria. More specifically, alteration in soil microbial communities can directly affect soil ecosystem functioning, particularly with respect to carbon and nitrogen cycles. Nitrogen can be a limiting nutrient, and the availability of nitrogen in the soil environment becomes a major factor in controlling the production of biomass. This research project aimed at studying the abundance of nitrogen-fixing, nitrogen-oxidizing, nitrogen-reducing, and ammonifying bacteria based on their functional genes in the tropical rain forests of Taman Nasional Bukit Duabelas (TNBD) and the oil palm plantations in Sarolangun Jambi. Samples were collected in November 2015. Soil sampling was performed randomly at three points representing each area of ​​the tropical rainforests of TNBD and the seven- to eight-year-old oil palm plantations. Soil samples were collected using a soil sample core from 0–15 cm below the surface with depth strata of 0–5 cm, 5–10 cm, and 10–15 cm. Composite assessment was conducted on samples from each point corresponding to each respective depth strata. Soil samples were stored at -20ºC prior to testing. Microbial abundance was measured using the most probable number (MPN) method. The abundance of microbes that play a role in nitrogen metabolism between strata of 5–10 cm and 10–15 cm does not appear to be different. The highest abundance of microbes in oil palm plantation land in Jambi was found in samples with nitrifying bacteria, later followed by denitrifying, nitrogen-fixing, and ammonifying bacteria. Ultimately, it was found that microbial abundance in oil palm plantations was higher than the corresponding rates in samples from tropical rainforests.
Estimasi Stok dan Serapan Karbon pada Mangrove di Sungai Tallo, Makassar Rahman Rahman; Hefni Effendi; Iman Rusmana
Jurnal Ilmu Kehutanan Vol 11, No 1 (2017)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.575 KB) | DOI: 10.22146/jik.24867

Abstract

Ekosistem mangrove memiliki kemampuan menyerap CO2 lebih tinggi dibandingkan dengan vegetasi tumbuhan lainnya. Namun upaya pengelolaannya sebagai kawasan penyimpan stok karbon masih belum maksimal. Kota Makassar memiliki Sungai Tallo yang sepanjang bantarannya ditumbuhi oleh vegetasi mangrove dan sangat potensial untuk dikelola sebagai ruang terbuka hijau. Hasil pengamatan menunjukkan bahwa Sungai Tallo terletak tepat di tengah kota Makassar dan sepanjang bantaran sungai didominasi oleh spesies Nypa fruticans dengan jumlah 18.514 pohon dan kerapatan 4.256 pohon/ha, menyimpan karbon sebesar 21,82 ton C/ha, menyerap 80,02 ton CO2/ha. Spesies dominan kedua adalah Rhizophora mucronata dengan jumlah 8.492 pohon dan kerapatan 2.352 pohon/ha, menyimpan karbon sebesar 19,94 ton C/ha, menyerap 73,13 ton CO2/ha. Spesies dominan ketiga yaitu Avicennia alba dengan jumlah 2.421 pohon dan kerapatan 3.228 pohon/ha, menyimpan karbon sebesar 53,96 ton C/ha, menyerap 197,87 ton CO2/ha. Nilai kerapatan dan kemampuan serapan mangrove tersebut sangat sesuai untuk dikelola pada ruang terbuka hijau penyuplai udara segar dan penyerap CO2.Stock Estimation and Carbon Absorption of Mangrove in Tallo River, MakassarAbstractThe mangrove ecosystem has a higher ability of CO2 absorption than other vegetations. However, the effort to establish the mangrove to be a carbon stock area has not been achieved. Makassar has Tallo River, covered with mangrove vegetation along its riverbank, which is potent to be managed as a green open space. The observations indicated that Tallo River was located in the center part of Makassar city and was dominated by Nypa fruticans along the riverbanks in 18,514 trees and a density of 4,256 trees/ha, stored carbon of 21.82 tons C/ha, and absorbs 80.02 tons CO2/ha. Rhizophora mucronata was the second dominant species in 8.492 trees and density of 2,352 trees/ha, stored carbon of 19.94 tons C/ha, and absorbs 73.13 tons CO2/ha. The third dominant species was Avicennia alba in 2,421 trees and density of 3,228 trees/ha, stored carbon of 263.85 tons C/ha, and absorbs 197.89 tons CO2/ha. The density and ability to absorb values of the mangrove is highly suitable to be managed for a green open space to supply fresh air and CO2.
Guide for Authors Iman Rusmana
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.053 KB) | DOI: 10.5454/mi.9.2.%p

Abstract

Subscription Form of Microbiology Indonesia Iman Rusmana
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.35 KB) | DOI: 10.5454/mi.9.2.%p

Abstract

Back Cover and Sponsor Information Iman Rusmana
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.97 KB) | DOI: 10.5454/mi.9.2.%p

Abstract

ITA registration form and Back Cover Iman Rusmana
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (961.495 KB) | DOI: 10.5454/mi.9.3.%p

Abstract

ITA REGISTRATION FORM AND BACK COVER Iman Rusmana
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1664.116 KB) | DOI: 10.5454/mi.13.1.%p

Abstract

Co-Authors Abdjad Asih Nawangsih ACENG RUYANI Achmad Dinoto Aderiana Masthura AKHADIYA, ALINA Alfan Cahyadi Alfisah, Rizal Khoirun Aliati Iswantari ALINA AKHDIYA Alina Akhdiya Alina Akhdiya Amanda, Nisa Widya Andi Ernawati Angela Mariana Lusiastuti Angela Mariana Lusiastuti Ani Suryani Ani Suryani Ani Widiyati ANIK KUSMIATUN Anja Meryandini Anja Meryandini Antonius Suwanto Apon Zaenal Mustopa Ari Fina Bintarti Ari Fina Bintarti, Ari Fina Aris Tri Wahyudi Armanisa, Khafifah Baskoro S Wibowo Cahyadi, Alfan Cecep Kusmana CECILIA ANNA SEUMAHU CECILIA ANNA SEUMAHU Ciptadi Achmad YUSUP Daniel Djokosetiyanto Darmono Taniwiryono Desniar - - Dewi Puspaningsih DIAH ISKANDRIATI Dian Hendrayanti Dinamella Wahjuningrum Dondin Sajuthi DWI ANDREAS SANTOSA Dwi Ningsih Susilowati Eddy Supriyono Emei Widiyastuti Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fahmi, Riziq Ilham Nur Faturrahman Faturrahman Ferbiyanto, Andri Fredinan Yulianda Frijona Fabiola Lokollo GALUH WENING PERMATASARI Gunawan Djajakirana Hamim Hamim Happy Widiastuti Hefni Effendi Hessy Novita Hessy Novita I Komang Gede Wiryawan I MADE ARTIKA I Made Samudra Ifa Manzila Ika Roostika Tambunan, Ika Roostika Ika Setianingsih, Ika Indriyani, Yohanna Anisa Irwan Cahyadi It Jamilah KATHARINA JESSICA Khairul Syahputra Kukuh Nirmala Kuntari, Wahyu Budi Lena Novita Lingga, Rahmad Lizawati . Maggy Thenawidjaya Suhartono Maggy Thenawidjaya Suhartono Mahdani, Wilda Mahyarudin, Mahyarudin Manguntungi, Baso Masrukhin Masrukhin Masthura, Aderiana Miftahudin . Mukti Ali MUNTI YUHANA MUTTAQIN, MAFRIKHUL Nedwell, David B. Niken T.M Pratiwi, Niken T.M NISA RACHMANIA MUBARIK Nisa Widya Amanda Novita, Lena Nurhakiki Nurjanah PRIHASTO SETYANTO Prihasto Setyanto Priyo Handoyo Wicaksono Rahman Rahman Rahman Rahman Rahminovita Herlis RATNA SETYANINGSIH RATNA SETYANINGSIH RATNANINGTYAS, SUSI RIDWAN AFFANDI Rika Indri Astuti RIKA RAFFIUDIN Rina Hesti Utami Risky Hadi Wibowo Risky Hadi wibowo Roedhy Poerwanto Rosadela, Fitri Sipriyadi Sipriyadi Sipriyadi Siti Nur Jannah Sobir Sobir Solly Aryza Sri Budiarti Poerwanto sri murtini . Suhartono Suhartono SUSAN SOKA Syaiful Anwar T. Widiyanto Taruna D. Satwika TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Thenawidjaya, Maggy Tri Eko WAHYONO Tri Handayani Kurniati Tri Handayani Kurniati Tri Muji Ermayanti Tri P. Priyatno Tri Panji Tri Puji Priyatno Tri Widiyanto Tridesianti, Siska Tuasalamony, Mutia Musdalifah Utut Widyastuti Wahyuni, Reza Wibowo, Risky Hadi WIDANARNI WIDANARNI Widiyanto, Tri Wilda Mahdani Wildan Nurusallam Wiraswati, Sri Martina Yadi Suryadi Yani Aryati Yohana A Dahliani Yohanna Anisa Indriyani Yuli Siti Fatma Yuli Siti Fatma YULIN LESTARI Yuni Puji Hastuti Yusli Wardiatno Zaenal Arifin, Sukarya Zulfarina Zulfarina Zulfarina,