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Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 , Lizawati; Roedhy Poerwanto; , Sobir; Iman Rusmana; Tri Muji Ermayanti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.098 KB) | DOI: 10.24831/jai.v35i2.1321

Abstract

Growth of mangosteen essentially depends on its root system.  Therefore, it needs technology to obtain stringer mangosteen root system.  The use of Agrobacterium rhizogenes bacterium is an alternative.  The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.  The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.  The experiment was arranged in completely randomized design with two factorial treatments.  The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).  The results showed that A. rhizogenes  of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.  Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method.   Key words :  Agrobacterium rhizogenes, Garcinia mangostana, inoculation
ISOLATION AND CHARACTERIZATION OF METHANOTROPHIC BACTERIA FROM RICE FIELDS Iman Rusmana; ALINA AKHADIYA
BIOTROPIA Vol. 16 No. 2 (2009): BIOTROPIA Vol. 16 No. 2 December 2009
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2009.16.2.53

Abstract

Methane is a greenhouse gas and has potency to deplete ozone layer.  Rice fields are a significantly sources of atmospheric methane.  Chemical fertilizers application in rice fields can increase methane emission. Methanotrophic bacteria are unique in their ability to utilize methane as a sole carbon and energy source.  This research was successfully isolating and characterizing methanotrophic bacteria from rice fields in Bogor and Sukabumi, West Java, Indonesia.  Determination of methane oxidation activity using Gas Chromatography showed that all isolates performed methane oxidation activity.  The highest methane oxidation activity was performed by BGM 9 isolate. And DNA amplification of BGM 9 genome was performed single band of mmoX in the size of 500 bp and three bands of pmoA  in the size of 1000, 750 and 500 bp respectively
Application of Biological Nitrogen Fixation Cyanobacteria To Paddy Plant Cultivated Under Deep-Water Culture System Dian Hendrayanti; Iman Rusmana; Dwi Andreas Santosa; Hamim Hamim
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.8510

Abstract

 The Biological Nitrogen Fixing (BNF) cyanobacteria can reduce atmospheric nitrogen into ammonium. This ability makes BNF cyanobacteria a potential eco-friendly N-source for soil-planted pad-dy. Apart from a few success stories of BNF cyanobacteria applica-tion in the rice field, its role as an ammonium producer is still an open question. There is also a possibility that indeed cyanobacteria biomass which provides nitrogen through the biological decomposing process. This study aimed to analyze the influence of three strains BNF cyanobacteria on paddy grown in the Deep-Water Culture (DWC) hydroponic system. Yoshida Nutrient Solution was used as a growth medium with the application of 0, 45.7, and 91.4 gL-1 of ammonium nitrate (NH4NO3) with five replications. The result showed that inter-action between cyanobacteria and nitrogen significantly influenced the total nitrogen of paddy plant, but not the paddy plant height, the num-ber of tillers, and biomass. Cyanobacterial filaments were found most-ly attached to the paddy root rather than floated in the DWC system. Paddy treated with cyanobacteria had more lateral root than control. The result suggested that cyanobacteria support paddy growth indi-rectly through nitrogen deposition in plant tissue and root development. 
Co-Authors Abdjad Asih Nawangsih Achmad Dinoto Aderiana Masthura Alfan Cahyadi Alfisah, Rizal Khoirun Aliati Iswantari ALINA AKHADIYA Alina Akhdiya Alina Akhdiya ALINA AKHDIYA Amanda, Nisa Widya Andi Ernawati Angela Mariana Lusiastuti Angela Mariana Lusiastuti Ani Suryani Ani Suryani Ani Widiyati ANIK KUSMIATUN Anja Meryandini Anja Meryandini Antonius Suwanto Apon Zaenal Mustopa Ari Fina Bintarti Ari Fina Bintarti, Ari Fina Aris Tri Wahyudi Armanisa, Khafifah Baskoro S Wibowo Cahyadi, Alfan Cecep Kusmana CECILIA ANNA SEUMAHU CECILIA ANNA SEUMAHU Ciptadi Achmad YUSUP Daniel Djokosetiyanto Darmono Taniwiryono Desniar - - Dewi Puspaningsih DIAH ISKANDRIATI Dian Hendrayanti Dinamella Wahjuningrum Dondin Sajuthi DWI ANDREAS SANTOSA Dwi Ningsih Susilowati Eddy Supriyono Emei Widiyastuti Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fahmi, Riziq Ilham Nur Faturrahman Faturrahman Ferbiyanto, Andri Fredinan Yulianda Frijona Fabiola Lokollo GALUH WENING PERMATASARI Gunawan Djajakirana Hamim Hamim Happy Widiastuti Hefni Effendi Hessy Novita Hessy Novita I Komang Gede Wiryawan I MADE ARTIKA I Made Samudra Ifa Manzila Ika Roostika Tambunan, Ika Roostika Indriyani, Yohanna Anisa Irwan Cahyadi It Jamilah KATHARINA JESSICA Khairul Syahputra Kukuh Nirmala Kuntari, Wahyu Budi Lena Novita Lizawati . Maggy Thenawidjaya Suhartono Maggy Thenawidjaya Suhartono Mahdani, Wilda Mahyarudin, Mahyarudin Manguntungi, Baso Masrukhin Masrukhin Masthura, Aderiana Miftahudin . Mukti Ali MUNTI YUHANA MUTTAQIN, MAFRIKHUL Nedwell, David B. Niken T.M Pratiwi, Niken T.M NISA RACHMANIA MUBARIK Nisa Widya Amanda Novita, Lena Nurhakiki Nurjanah PRIHASTO SETYANTO Prihasto Setyanto Priyo Handoyo Wicaksono Rahman Rahman Rahman Rahman Rahminovita Herlis RATNA SETYANINGSIH RATNANINGTYAS, SUSI RIDWAN AFFANDI Rika Indri Astuti RIKA RAFFIUDIN Rina Hesti Utami Risky Hadi Wibowo Risky Hadi wibowo Roedhy Poerwanto Rosadela, Fitri Sipriyadi Sipriyadi Sipriyadi Siti Nur Jannah Sobir Sobir Sri Budiarti Poerwanto sri murtini . Suhartono Suhartono SUSAN SOKA Syaiful Anwar T. Widiyanto Taruna D. Satwika TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Thenawidjaya, Maggy Tri Eko WAHYONO Tri Handayani Kurniati Tri Handayani Kurniati Tri Muji Ermayanti Tri P. Priyatno Tri Panji Tri Puji Priyatno Tri Widiyanto Tridesianti, Siska Tuasalamony, Mutia Musdalifah Utut Widyastuti Wibowo, Risky Hadi WIDANARNI WIDANARNI Widiyanto, Tri Wildan Nurusallam Wiraswati, Sri Martina Yadi Suryadi Yani Aryati Yohana A Dahliani Yohanna Anisa Indriyani Yuli Siti Fatma Yuli Siti Fatma YULIN LESTARI Yuni Puji Hastuti Yusli Wardiatno Zaenal Arifin, Sukarya Zulfarina Zulfarina Zulfarina,