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Isolation and Identification of Profenofos Pesticide-Tolerant Bacteria from Agriculture Land in Sleman Regency Pertiwi, Melania Citra; Rakhmawati, Anna; Octavia, Bernadetta; Aminatun, Tien; Suhartini
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

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Abstract

The intensive application of organophosphate pesticides containing profenofos as the active compound may lead to soil contamination and the emergence of pesticide-tolerant bacterial strains with potential for bioremediation. Comprehensive identification and characterization of these bacteria are crucial to support sustainable agricultural and environmental management practices. The aim of the study is to analyze the characteristics and identify indigenous bacteria tolerant to profenofos pesticide in Sleman agricultural land, DIY. This study is qualitative research using an exploratory-descriptive. Isolation was carried out by dilution to a level of 10-7 followed by a spread plate on Mineral Salt Medium (MSM) which was given with 100 ppm profenofos pesticide. Bacterial isolates were characterized and then identified using profile matching with reference genera. Based on the results of the study, it is found that 10 bacterial isolates tolerant to organophosphate pesticide with the active ingredient profenofos that have a similarity index of ≥70%. Based on the reference bacteria, 5 different genera are obtained, namely Paracoccus (D3, D8), Micrococcus (D5, D6, D9), Enterococcus (D2, D10), Arthrobacter (D1, D4), and Neisseria (D7). The bacterial isolates obtained have different characteristics. The characteristics of these bacteria are gram positive and negative bacteria with round, irregular, and rhizoid shapes. Their margins are lobate, curled, entire, undulate, and ciliate. Their elevations are umbonate, raised, and flat. The color of the bacteria is white to light orange, with various physiological test results.
Reduction of the Heavy Metal Mercury (Hg) from Paningkaban Gold Mining Tailings, Bayumas, with Ageratum Conyzoides Althaaf, Nabila Tsabita; Aminatun, Tien; Rakhmawati, Anna; Atun, Sri
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Gold is a fairly large contributor to Indonesia's foreign exchange. The area that has the potential to have minerals is Banyumas Regency. This area has been indicated to have gold mineral reserves of around 16,000 hectares. Until now, the gold processing process still uses mercury, which produces negative impacts such as post-mining waste containing heavy metal mercury (Hg), which can cause environmental damage. Environmental damage that arises, such as pollution of the soil and water environment, due to the gold bioleaching process that is disposed of without any treatment. One alternative that can be used to overcome this problem is by using hyperaccumulator plants. One plant that falls into the hyperaccumulator category is the bandotan plant (Ageratum conyzoides), because it is often found in gold mining areas contaminated with mercury. Mercury contamination will be absorbed from the environment into the body of Ageratum conyzoides through membranes and converted into less toxic substances. The aim of this research is to determine the ability of the Ageratum conyzoides plant as a phytoremediation agent in reducing mercury metal (Hg) in tailings from Paningkaban Village, Banyumas. There are two treatments, namely the planting media used with 2 kg of tailings soil and 2 kg of planting media mixed with tailings and compost. The mixture ratio between tailings and compost is 1:1. Each treatment, consisting of three replications, was observed using plant growth parameters (plant height and number of leaves), as well as control parameters (edaphic and microclimatic). The results of the research show that the Ageratum conyzoides plant is a phytoremediation agent that can reduce levels of the heavy metal mercury (Hg) in the planting medium of mining tailings soil from Paningkaban Village, because it gives test results of BCF values > 1 and TF < 1, which indicates that the Ageratum conyzoides plant has potential as a phytostabilizing agent.
Co-Authors - Suyanta - Yuliati - Yuliati A.A. Ketut Agung Cahyawan W Abdullah Nashih Amin Agung Lesmana Agung Ramadhan Agus Widyianto Aisya, Naafi Ajeng Septiana Putri Aji Suhandy Aji Suhandy Althaaf, Nabila Tsabita Amanina khusna Maulida Amin Khusnadiyah ana arifatul ummahz Anggraeni, Titis Nindiasari Anggun Andreyani Anisa Setya Hidayah Anna Rakhmawati Arifudin Idrus Arrozi, Wahyu Asri Widowati Aulia, Fera Azizah Dila Novianti Azizah Dila Novianti Baiki, Khomsa Amar Baso Samsu Rijal Bernadetta Octavia Budiwati Budiwati Budiwati Budiwati Daryono, Rihab Wit Daud Dakabesi David Meilana Nur Arifin Diah Tirta Puri Dina Ayu Kiswandini Djuwanto Djuwanto Dwi Astuti Dwi Novita, Ria Dwiana Muflihah Yulianti Dwiki Prasetiya Prayudi Edhi Martono Edhi Martono Ema Imtihana Endah Ratna Sari Farida Hanum Feliksitas Angel Masing Fitra Purnama Agung Fitri Nurfatonah Fitri Rahmawati Fitri Rahmawati Galuh Danang Sumari gendra devi putra achir Habibatul Unayah Heru Kuswanto Hutama, Ponty S.P. I Gusti Putu Suryadarma, I Gusti Putu Ibnu Pratomo Ibrahim, Fardan Yusuf Indah Kristiani Siringo Ringo Insih Wilujeng Ishadiyanto Salim Junita Kurniawati Kim Jung Bog Kun Sri Budiasih Kurnia Cahyani Kuswarsantyo Kuswarsantyo Lili Sugiyarto Lukman Ari Bahrtiar Marfuatun, Marfuatun muhammad irfan Muhammad Rasyid Mutia Ulfah Naafi Aisya Nrangwesthi Widyaningrum Nugroho Susetya Nugroho Susetya Putra Nur Rizki Putri Ramadhanti Nurul Endah Rahmawati1 Paramita Cahyaningrum Kuswandi Paryanto Pertiwi, Melania Citra Putri, Rizka Apriani Ramadhanti, Nur Rizki Putri Ratnawati Ratnawati Regina Tutik Padmaningrum Reginaldis Isabella Sado Reza Fahlevi Rizka Apriani Putri Rizky Diah Aryani Rizky Diah Aryani Roin Fathul Arzaqi S. Djalal Tanjung S. Djalal Tanjung, S. Djalal Sabar Nurohman Sanudin Sanudin satrio haryo pamungkas Setyo Sulistyono Simbolon, Doly Siti Ainun Jariah Siti Umniyatie Siwi Indarti Slamet Suyanto SRI ATUN Sri Harti Widyastuti Sudarsono Sudarsono Sugma Suhartini Suhartini Suhartini Suhartini Suhartini Suhartini Sujangka, Arsyad Sulistiyawati Sulistiyawati Sulistiyawati Sulistiyawati Sunarta Suratman Woro Suratman Woro Suratsih Suratsih Triatmanto Triatmanto Tutiek Rahayu Tutiek Rahayu Tutik Utika Sari Utami, Yanika Cahya Vichit Rangpan Victoria Henuhili Victoria Henuhili Wening Kusumawardani Wibowo, Yuni Yanuarief, Cecilia Yoga Guntur Sampurno yoyon arifta Yuliati Yulianti Yuliati Yuliati Yuni Prastiwi Mutiarani Yuni Wibowo Yuni Wibowo Zaenuddin Hudi Prasojo