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Isolation and Identification of Profenofos Pesticide-Tolerant Bacteria from Agriculture Land in Sleman Regency Pertiwi, Melania Citra; Rakhmawati, Anna; Octavia, Bernadetta; Aminatun, Tien; Suhartini
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

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Abstract

The intensive application of organophosphate pesticides containing profenofos as the active compound may lead to soil contamination and the emergence of pesticide-tolerant bacterial strains with potential for bioremediation. Comprehensive identification and characterization of these bacteria are crucial to support sustainable agricultural and environmental management practices. The aim of the study is to analyze the characteristics and identify indigenous bacteria tolerant to profenofos pesticide in Sleman agricultural land, DIY. This study is qualitative research using an exploratory-descriptive. Isolation was carried out by dilution to a level of 10-7 followed by a spread plate on Mineral Salt Medium (MSM) which was given with 100 ppm profenofos pesticide. Bacterial isolates were characterized and then identified using profile matching with reference genera. Based on the results of the study, it is found that 10 bacterial isolates tolerant to organophosphate pesticide with the active ingredient profenofos that have a similarity index of ≥70%. Based on the reference bacteria, 5 different genera are obtained, namely Paracoccus (D3, D8), Micrococcus (D5, D6, D9), Enterococcus (D2, D10), Arthrobacter (D1, D4), and Neisseria (D7). The bacterial isolates obtained have different characteristics. The characteristics of these bacteria are gram positive and negative bacteria with round, irregular, and rhizoid shapes. Their margins are lobate, curled, entire, undulate, and ciliate. Their elevations are umbonate, raised, and flat. The color of the bacteria is white to light orange, with various physiological test results.
Reduction of the Heavy Metal Mercury (Hg) from Paningkaban Gold Mining Tailings, Bayumas, with Ageratum Conyzoides Althaaf, Nabila Tsabita; Aminatun, Tien; Rakhmawati, Anna; Atun, Sri
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

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Abstract

Gold is a fairly large contributor to Indonesia's foreign exchange. The area that has the potential to have minerals is Banyumas Regency. This area has been indicated to have gold mineral reserves of around 16,000 hectares. Until now, the gold processing process still uses mercury, which produces negative impacts such as post-mining waste containing heavy metal mercury (Hg), which can cause environmental damage. Environmental damage that arises, such as pollution of the soil and water environment, due to the gold bioleaching process that is disposed of without any treatment. One alternative that can be used to overcome this problem is by using hyperaccumulator plants. One plant that falls into the hyperaccumulator category is the bandotan plant (Ageratum conyzoides), because it is often found in gold mining areas contaminated with mercury. Mercury contamination will be absorbed from the environment into the body of Ageratum conyzoides through membranes and converted into less toxic substances. The aim of this research is to determine the ability of the Ageratum conyzoides plant as a phytoremediation agent in reducing mercury metal (Hg) in tailings from Paningkaban Village, Banyumas. There are two treatments, namely the planting media used with 2 kg of tailings soil and 2 kg of planting media mixed with tailings and compost. The mixture ratio between tailings and compost is 1:1. Each treatment, consisting of three replications, was observed using plant growth parameters (plant height and number of leaves), as well as control parameters (edaphic and microclimatic). The results of the research show that the Ageratum conyzoides plant is a phytoremediation agent that can reduce levels of the heavy metal mercury (Hg) in the planting medium of mining tailings soil from Paningkaban Village, because it gives test results of BCF values > 1 and TF < 1, which indicates that the Ageratum conyzoides plant has potential as a phytostabilizing agent.
EFFECTIVENESS OF MAJAPAHIT LEAF EXTRACT (Crescentia cujete L.) AS A NATURAL PESTICIDE FOR BANANA LEAF ROLLER CATERPILLAR (Erionota thrax L). Pratomo, Ibnu; Aminatun, Tien; Kuswandi, Paramita Cahyaningrum
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.106

Abstract

This study aims to determine the effectiveness of Majapahit leaf extract (Crescentia cujete L.) on the feeding activity and mortality of the banana leaf roller caterpillar (Erionota thrax L.), and also the optimal concentration that affects the feeding activity and mortality of the banana leaf roller caterpillar (Erionota thrax L.). This research is a completely randomized design (CRD) experiment with 4 treatments and 3 replicates. In thos study, 60 third instar larvae of Erionota thrax L. were taken using the transect method from several villages in Purworejo district, Central Java. The pesticide from Majapahit leaves was made through a simple extraction method with 4 concentrations: 0%, 25%, 30% and 35%. The results showed that Majapahit leaf extract had an effect on feeding activity and mortality of the banana leaf rolling caterpillar. The optimal concentration of Majapahit leaf extract that affects feeding activity and pest mortality was 35%.
A Systematic Review of Pedagogical Models to Enhance Students’ Critical Thinking in Physics Learning Rasyid, Muhammad; Unayah, Habibatul; Kuswanto, Heru; Aminatun, Tien; Sulistiyawati, Sulistiyawati; Bog, Kim Jung
Jurnal Pendidikan MIPA Vol 25, No 3 (2024): Jurnal Pendidikan MIPA
Publisher : FKIP Universitas Lampung

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Critical thinking skills are important skills needed in the 21st century  Critical thinking skills can be trained in students through student-centered learning  This research aims to identify learning models that can improve physics critical thinking skills through a literature review  Data collection is based on Scopus and SINTA database sources  This type of document comes from magazine articles tagged "critical thinking", "learning models", "physics learning" for the year (2019-2024)  Methods adapted from PRISMA were used for the literature search  Published documents are based on predetermined content analysis criteria, such as year of publication, author, article source, learning model, physical material topic, research topic, education level, and evaluation  Inquiry learning and higher order thinking laboratories (HOT Labs) are the dominant learning models used to improve critical thinking skills  The main competency indicators used vary  Indicator of critical thinking abilities  Self-regulated learning is rarely used in research tools compared to other indicators of critical thinking abilities  The instruments used are multiple choice questions, essays, worksheets and observation sheets  University-level research participants often use critical thinking skills in their research  Fluid mechanics is a popular topic in research  This research will help improve students' critical thinking skills through various recommended learning models  Research shows that student-centered learning models can improve critical thinking skills.        Keywords: critical thinking skills, fluid mechanics, guided inquiry, learning models, physics learning.DOI: http://dx.doi.org/10.23960/jpmipa/v25i3.pp1444-1467
Development of Smart Apps Creator Learning Media Using Problem-Solving Learning Models on Global Warming Materials to Improve Critical Thinking and Problem-Solving Ability Fahlevi, Reza; Aminatun, Tien
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.4311

Abstract

This study aims to 1) determine the feasibility of smart apps creator-based learning media used in learning activities, and 2) determine the effectiveness of Smart Apps Creator-based learning media to improve Critical Thinking and Problem Solving skills in Global Warming Materials for Class X Students of SMAN 4 Berau. This research is a Research and Development (R&D) study which refers to the ADDIE model. This research was conducted on 30 students in limited trials and field trials using class X1 and X3 students each of 32 students. The results of the study are that the learning media developed meet the eligibility based on expert judgment and effective learning media in improving critical thinking skills and problem solving abilities. The results of the Manova test show a sig value of 0.000 <0.05, namely Ha is accepted so that there are differences in critical thinking skills and problem-solving abilities between students who use Smart Apps Creator learning media and PowerPoint learning media. The N-gain test of learning media on critical thinking skills gets a score of 68.82 which means it is quite effective and for problem solving skills it gets a score of 91.64 which means it is effective.
Analisis Kemampuan Pemecahan Masalah Biologi Materi Perubahan Lingkungan Di Sman 4 Yogyakarta Sugma; Aminatun, Tien; Kiswandini, Dina Ayu
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.4447

Abstract

To solve problems accurately and focused, students must have problem solving skills. The purpose of this study was to evaluate class X students of SMA N 4 Yogyakarta in the even semester of the academic year. This study uses a descriptive quantitative research method. As a sample used simple random sampling. According to Polya, there are four indicators of problem-solving ability: Understanding the problem, preparing, implementing plans, and re-checking are steps that must be taken. On the topic of environmental change, the test instrument revealed that students' problem-solving abilities were in the high range. But if we look at each indicator separately, we can see that all four of them belong to a group. This is because students do not double-check their written answers and continue to doubt these answers. The non-test instrument study, on the other hand, the non-test instrument study found that students' problem solving abilities were very good. Based on these findings, it can be concluded that the students' overall problem solving abilities are of a high standard. Because research findings cannot be used with all materials, problem solving skills must be tested with different materials.
Development of OEFPADU Syntax Biology LKPD to Improve Critical Thinking of Islamic Values and Scientific Attitud Sanudin, Sanudin; Aminatun , Tien
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4733

Abstract

This study aims to produce Biology LKPD, determine the feasibility and effectiveness of the LKPD developed to increase critical thinking of Islamic value and Scientific attitude. The method used in this research is Research and Development (R & D) with the ADDIE model (Analysis, Design, Development, and Implementation, Evaluation) developed by Dick & Carry. Data analysis techniques used gain scores (Statistic analysis). Evaluation of biological LKPD products according to product feasibility experts is in the good category. According to the biology teacher, it was in the outstanding category and the students' responses were in the outstanding category. Learning using the OEFPADU LKPD syntax shows that critical thinking of Islamic values increases (the average gain score is 0.72), independent test simple t test sign. 0.000 and paired sample t-test 0.001 and observations of 7.60%. The scientific attitude of students increased by 14.30%, from the good to outstanding category with the Wiloxcon test analysis, the scientific attitude before and after using the OEFPADU syntax LKPD there was a significant difference with the Asymp results. Sig. 0.000 (<0.05). This shows that the use of OEFPADU LKPD syntax is effective in improving critical thinking skills of Islamic values ​​and scientific attitudes.
PBL dalam Blended Learning untuk Meningkatkan Kemampuan Berpikir Kritis dan Pemecahan Masalah Kusumawardani, Wening; Aminatun, Tien
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7052

Abstract

This study explores how PBL integrated into blended learning affects the enhancement of critical thinking and problem-solving skills among senior high school students. It employs a quasi-experimental design involving a non-equivalent pretest-posttest control group. Participants consist of students from class X-5 assigned to the experimental group and students from class X-8 assigned to the control group. Data were collected using a validated description test to measure critical thinking and problem-solving skills. Analysis involved MANOVA, N-Gain calculation, and the Pearson correlation test. Findings indicate that implementing PBL in blended learning effectively improves critical thinking and problem-solving abilities. The experimental group's average post-test score for critical thinking was 85.83, compared to 75.42 in the control group, and for problem-solving, it was 85.56, compared to 71.67 in the control group.
Pengembangan Media Pembelajaran Interaktif pada Materi Sistem Endokrin untuk Meningkatkan Motivasi Belajar Siswa Kelas XI SMA Sari, Tutik Utika; Aminatun, Tien
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.8465

Abstract

This research aims to produce interactive learning media that is feasible, practical and effective to use on endocrine system material to increase learning motivation for class XI high school students. This research uses the method Research and Development (R&D) with the Analysis, Design, Development, Implementation, and Evaluation (ADDIE) development model. The research was conducted at SMA Negeri 1 Minggir on class XI MIPA students consisting of 2 classes, namely the control class and the experimental class with a total of 36 students each. The data collection instruments used were material expert and media expert validation sheets, biology teacher assessment sheets, student response sheets and non-test instruments in the form of questionnaires. Analysis of learning motivation data was calculated using the independent sample t˗test with a significance level of 0.05. The average learning motivation obtained in the experimental class was 89.98 with an N-gain value of 0.75 in the high category, while the average learning motivation in the control class was 59.72 with an N-gain value of 0.55, in the medium category. Based on the results of the Independent Sample t-test, the results obtained were 0.000 < 0.05 and it was stated that media use interactive learning proven effective in increasing student learning motivation.
PEMECAHAN MASALAH LIMBAH LAUNDRY DI SLEMAN DENGAN PROGRAM IPTEK BAGI MASYARAKAT (IbM) Aminatun, Tien; Padmaningrum, Regina Tutik; Yuliati, -
INOTEKS: Jurnal Inovasi Ilmu Pengetahuan,Teknologi, dan Seni Vol 20, No 1 (2016)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.557 KB) | DOI: 10.21831/ino.v20i1.11273

Abstract

Karangmalang and Kuningan are the area located near the campus ofYogyakarta State University and Gadjah Mada University. This causes the twoareas have crowded population. Many students from outside of Sleman Yogyakarta stay in many boarding houses that need laundry service since they do not have enough time and are very busy of studying activities. There are approximately 30 laundry service businesses in these areas. The condition has positive impact on local people income generating. However, it also cause adverse effects on the environment because of the laundry waste water directly discharged into the environment without prior treatment. The priority problem to solve is the disposal of waste water and dissemination of eco-friendly detergen. We had "Sekar Laundry" and "Ramadhani Laundry"as our partners in this programme. To achieve the objectives of the project we carried out the following methods: (1) socialization, counseling, and training for the introduction of ecofriendly detergents and simple sewage treatment; and (2) the application of appropriate technology of waste water treatment based on fitoremediation for both partners. The results obtained were in accordance with the purposes. We could overcome the environmental pollution caused by waste water of laundry activities. Based on laboratory tests, laundry waste water from the outlet of the Waste Water Treatment Plant (the WWTP) had significantly quality improvement that it made safer to be discharged into the environment, with 27,125% of BOD decline efficiency, 120,350% of COD decline efficiency, 17,300% of TSS decline efficiency, 11,597% of detergent decline efficiency, and 88% of phosphate decline efficiency 
Co-Authors - Suyanta - Yuliati -, Paryanto A.A. Ketut Agung Cahyawan W Abdullah Nashih Amin Agung Lesmana Agung Ramadhan Agus Widyianto Aisya, Naafi Ajeng Septiana Putri Aji Suhandy Aji Suhandy Ali Mahmudi Althaaf, Nabila Tsabita Amanina khusna Maulida Amin Khusnadiyah ana arifatul ummahz Andreyani, Anggun Anggraeni, Titis Nindiasari Anisa Setya Hidayah Anna Rakhmawati Arifudin Idrus Arrozi, Wahyu Arsyad Sujangka Asri Widowati Aulia, Fera Azizah Dila Novianti Azizah Dila Novianti Baiki, Khomsa Amar Baso Samsu Rijal Bayu Setiaji Bernadetta Octavia Bog, Kim Jung Budiwati Budiwati Budiwati Budiwati Budiwati Budiwati Budiwati Dadan Rosana Damayanti, Anastasya Daryono, Rihab Wit Daud Dakabesi David Meilana Nur Arifin Diah Tirta Puri Djuwanto Djuwanto Dwi Astuti Dwi Ayu Nurfaizah Dwi Novita, Ria Dwiana Muflihah Yulianti Dwiki Prasetiya Prayudi Edhi Martono Edhi Martono Ema Imtihana Endah Ratna Sari Fardan Yusuf Ibrahim Farida Hanum Fatin, Rahma Nabila Feliksitas Angel Masing Fitra Purnama Agung Fitri Nurfatonah Fitri Rahmawati Fitri Rahmawati Fitriani Fitriani Galuh Danang Sumari gendra devi putra achir Genius Abid Karunia Hasna Kamila Hanum Heru Kuswanto I Gusti Putu Suryadarma, I Gusti Putu Ibrahim, Fardan Yusuf Indah Kristiani Siringo Ringo Insih Wilujeng Ishadiyanto Salim Junita Kurniawati Kiswandini, Dina Ayu Kun Sri Budiasih Kurnia Cahyani Kusumawardani, Wening Kuswarsantyo Laifa Rahmawati Lili Sugiyarto Lukman Ari Bahrtiar Marfuatun . Mualimin Mualimin muhammad irfan Muhammad Rasyid, Muhammad Mutia Ulfah Naafi Aisya Nilahazra Khoirunnisa Nrangwesthi Widyaningrum Nugroho Susetya Nugroho Susetya Putra Nur Rizki Putri Ramadhanti Nurul Endah Rahmawati1 Paramita Cahyaningrum Kuswandi Paryanto Pertiwi, Melania Citra Ponty S.P. Hutama Pratiwi, Vicha Rista Pratomo, Ibnu Pujianto Raharjo, Rahmanto Rahmania Pamungkas Ramadhanti, Nur Rizki Putri Rangpan, Vichit Rania Nur Farida Ratnawati Ratnawati Regina Tutik Padmaningrum Reginaldis Isabella Sado Reza Fahlevi REZA FAHLEVI Rizka Apriani Putri Rizky Diah Aryani Rizky Diah Aryani Roin Fathul Arzaqi S. Djalal Tanjung S. Djalal Tanjung, S. Djalal Sabar Nurohman Sanudin Sanudin Sari, Tutik Utika satrio haryo pamungkas Setyo Sulistyono Simbolon, Doly Siti Ainun Jariah Siti Umniyatie Siwi Indarti Slamet Suyanto SRI ATUN Sri Harti Widyastuti Sudarsono Sudarsono Sugma Suhartini Suhartini Suhartini Suhartini Suhartini Sujangka, Arsyad Sulistiyawati Sulistiyawati Sunarta Sunarta - Suratman Woro Suratman Woro Suratsih Suratsih Syfa Trinandita Tarigan, Wenny Pinta Litna Triatmanto Triatmanto Tutiek Rahayu Tutiek Rahayu Unayah, Habibatul Utami, Yanika Cahya Victoria Henuhili Victoria Henuhili Wibowo, Yuni Yanuarief, Cecilia Yoga Guntur Sampurno yoyon arifta Yuliati Yulianti Yuliati Yuliati Yuni Prastiwi Mutiarani Yuni Wibowo Yuni Wibowo Yusman Wiyatmo Zaenuddin Hudi Prasojo