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All Journal Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Pengembangan Kota Jurnal Medik Veteriner Martabe : Jurnal Pengabdian Kepada Masyarakat Majalah Farmasetika Jurnal Gema Ngabdi Advances in Tropical Biodiversity and Environmental Sciences Nusantara Science and Technology Proceedings Jurnal Pengabdian Kepada Masyarakat Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Journal of Veterinary and Animal Sciences Jurnal Medika Veterinaria
I Made Merdana
Laboratorium Farmakologi Dan Farmasi Veteriner, Universitas Udayana
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Kadar Total Protein Serum Sapi Bali Pasca Transportasi di Rumah Pemotongan Hewan Pesanggaran Denpasar Gusti Agung Rama Wiratama Putra; Iwan Harjono Utama; I MAde Merdana
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p16

Abstract

This study aims to determine the effect of transportation on changes in total protein levels in Bali cattle after a trip to the Pesanggaran Slaughterhouse (RPH) Denpasar. Transportation can lead to stress and dehydration. To diagnose the occurrence of stress can be done by biochemical tests. The biochemical test carried out in this study was to measure the levels of total protein in the blood. The object used is Bali cattle that have not been rested after transportation as many as 20 heads at the Denpasar Pesanggaran Slaughterhouse. Blood was taken through the auricular vein and put into a vacutainer tube containing a gel separator to separate serum from blood. Blood serum samples were taken to the Bali Provincial Health Laboratory for immediate examination of total protein levels using the Photometer 5010V5+ machine. The total protein content of the examined samples had the lowest level of 5.8 g/dL and the highest 8.5 g/dL with an average of 7.5 g/dL. From the results of the study, the total protein content of Bali cattle after transportation was considered normal, this indicated that there was no effect of transportation on the total protein content.
Histopatologi Hati Tikus Wistar Periodontitis Apikalis Kronis Pasca Pemberian Medikamen Intrakanal Theresia Ene; I Made Merdana; Ida Bagus Oka Winaya; Ni Kadek Eka Widiadnyani; Luh Made Sudimartini
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p15

Abstract

Dental disease is a problem that is common in Indonesia, and it affects around 96.58% of the population. The use of chemical drugs that can cause side effects in patients makes people start other alternative treatments by using natural ingredients namely meniran (Phyllanthus niruri L). This study examines the liver histopathology of Wistar rats with chronic apical periodontitis after administration of intracanal drugs. This study looked at the effects of a medicament paste on rats for a period of seven, fourteen, and twenty-one days. Experimental animals were adapted to chronic apical periodontitis by inducing Enterococcus faecalis into right maxillary molars for 21 days.On day 22, all drug treatment groups were opened with GIC and given the prescribed drug treatment, and then closed again with GIC. The observed variables were the presence of inflammatory cell infiltration, hyperemia, and necrosis. Data were analyzed using a non-parametric statistical Kruskal-Wallis test, and if there was a significant difference, it was continued with the Mann-Whitney test. The results of the study showed that there was no significant difference between the three treatment groups (P1, P2, P3) and there was a significant difference between P0 and the other two groups. However, the results of statistical analysis of necrosis observed by histopathology in the liver showed that there was no significant difference between P0, P1, P2 and P3 treatments (P>0.05).From the research results, it can be concluded that administration of meniran leaves ethanolic extract can improve liver histopathology in rats with chronic apical periodontitis.
Pengaruh Ekstrak Kayu Secang Terhadap Gambaran Histopatologi Ginjal Mencit Jantan Pasca Paparan Asap Rokok Konvensional Yoga Pratama Mambela Sarungallo; I Ketut Berata; I Made Merdana
Buletin Veteriner Udayana Vol. 15 No. 3 June 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i03.p13

Abstract

Indonesia is one of the developing countries that has a high number of smokers and cigarette production. Cigarettes are not only harmful to the smoker, but also harmful to those around them who inhale the smoke. Organ damage caused by free radicals from cigarette smoke can be repaired with antioxidants. One of the plants in Indonesia that contains antioxidants is sappan wood (Caesalpinia sappan linn). This study aims to determine the effect of sappan wood extract on the histopathological picture of male mice (Mus musculus) exposed to conventional cigarette smoke for 30 days. This study used kidney samples of male mice, adults aged 35-45 days and body weight between 30-35g totaling 24 individuals. Mice were divided into 4 groups, each group consisted of 6 mice. The experimental animals were isolated on the 30th day, then the kidney histopathological examination of mice was performed. The results of histopathological observations of male mice's kidneys showed varying degrees of severity. The results of Kruskal Wallis' analysis of histopathological examination data were scored, showing a significant difference (p<0.05) for both necrotizing and inflammatory lesions. From the results of the study, it can be concluded that administration of secang wood extract at a dose of 30 mg/30 g BW of mice/day and 60 mg/30 g BW of mice/day can improve kidney lesions of male mice due to exposure to cigarette smoke, based on the reduction of necrotizing and inflammatory lesions. Further research is needed with increasing doses and longer time to determine the effective dose of secang wood extract in reducing kidney damage due to exposure to cigarette smoke in mice.
Pengaruh Ekstrak Kayu Secang Terhadap Gambaran Hispatologi Jantung Mencit Jantan Pasca Paparan Asap Rokok Konvensional Franky Samuel Milenyano Chandra; I Ketut Berata; I Made Merdana
Buletin Veteriner Udayana Vol. 15 No. 4 August 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i04.p20

Abstract

Exposure to cigarette smoke can increase the risk of coronary heart disease due to the free radicals generated. One of the plants in Indonesia that contains antioxidants is sappan wood. The purpose of this study was to determine the effect of giving secang wood extract (Caesalpinia sappan Linn) on the histopathological picture of male mice (Mus musculus) after exposure to conventional cigarette smoke. This study used 24 samples of adult male mice aged 35-45 days with body weight between 30-35 grams obtained from a mouse farm in Gianyar, Bali. The sample was divided into 4 treatments with 6 replications, namely negative control without sappan extract treatment and exposure to cigarette smoke, positive control with 2 cigarette smoke exposure a day, 2 cigarette smoke exposure a day and 30 mg/head dose of sappan wood extract. and the treatment was exposed to cigarette smoke as much as 2 cigarettes a day and administration of sappan wood extract at a dose of 60 mg/head, for 30 days. On the 31st day, the experimental animals were sacrificed to take heart muscle and processed into histopathological preparations. The examination was carried out based on the presence of congestion and inflammation lesions. The severity of the lesions was scored as mild, moderate and severe. Observational data were analyzed using the Kruskal-Wallis non-parametric statistical test and continued with the Mann-Whitney test if there was a significant difference. The results showed that there was a significant difference (p<0.05) between mice exposed to cigarette smoke and mice given sappan wood extract after exposure to cigarette smoke. It can be concluded that the administration of sappan wood extract at a dose of 30mg/mouse and 60mg/mouse can improve the heart lesions of male mice after exposure to conventional cigarette smoke. Further research is needed on the use of sappan wood extract with different doses in order to obtain more comprehensive results in treating heart damage after exposure to cigarette smoke.
Gambaran Histopatologi Paru-Paru Ayam Kampung Jantan yang Diberikan Minyak Rajas I Putu Dwi Komala Putra; I Made Merdana; Luh Gde Sri Surya Heryani
Buletin Veteriner Udayana Vol. 15 No. 3 June 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i03.p01

Abstract

Minyak Rajas is a natural medicine formulated from various herbal plants containing activeingredients with pharmacological activity. This study aims to determine the effect of Minyak rajas onthe lungs of male free-range chickens. The study used a completely randomized design with fourtreatments, namely control and three doses of Minyak rajas orally. A total of 24 male free-rangechickens weighing 500-600grams were randomly divided into four treatments with six replications.As a control in the P0 group, they were fed a placebo with distilled water instead of Minyak rajas.Minyak Rajas treatment in groups P1, P2 and P3 respectively with doses of 92,3mg/kg, 184,6mg/kgand 369,2mg/kg mixed with feed for six weeks. Data analysis using the Mann-Whitney test showedthat the histopathological features of the lungs found in the form of fatty degeneration, inflammatorycell infiltration, bleeding and necrosis were not significantly different (P>0.05) in all treatments. It canbe concluded that the administration of Minyak Rajas has no effect on changes in thehistopathological picture of the lungs of male free-range chickens. Further research can be done toincrease the dose of giving Minyak Rajas orally to determine the dose limit of giving rajas oil andusing more accurate parameters in identifying damage to an organ.
Artikel Riview: Penanganan dan Pencegahan Kejadian Histomoniasis pada Unggas Domingas Pereira; I Made Merdana; Ida Bagus Komang Ardana
Buletin Veteriner Udayana Vol. 15 No. 3 June 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i03.p08

Abstract

Histomoniasis is a protozoan parasitic disease caused by Histomonas meleagridis. Clinical signs in histomoniasis develop 6-12 days post-infection, and are most common at 11 days post-infection, and include lethargy, emaciation, unkempt hair, yellow diarrhea, sulphur-like stools in the late stages of the disease when liver function is impaired. severe, and bile pigment is excreted through the kidneys. Lesions are observed mainly in the liver and cecum. Typical liver lesions are described as necrosis that is yellow to gray, green, or red. The size of the lesions varies widely, but is usually 1–2 cm in diameter, and may coalesce to form larger areas of necrosis. The cecum is most often enlarged bilaterally, with thickening of the cecum walls. The diagnosis can be made on the basis of clinical signs and characteristic lesions of the liver and/or cecum. A well-developed target lesion in the liver +/- cecum lesion is pathognomonic for this disease. Since no approved chemotherapy product is available for the treatment of histomoniasis, control measures are focused on prevention. There are no approved chemotherapy products available for the treatment of histomoniasis, so control measures are focused on prevention. To prevent the occurrence of histomoniasis, the best prevention that can be done is to apply good sanitation in livestock cages.
Changes in blood calcium concentrations in Bali cattle during the periparturient period I Made Merdana; I Kadek Ariyuda Prasetya; I Nyoman Sulabda; Anak Agung Gde Arjana; Samsuri Samsuri
Journal of Veterinary and Animal Sciences Vol 3 No 2 (2020)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2020.v03.i02.p03

Abstract

Calcium is a macromineral that plays an important role in the health, production, and reproduction of animals. Calcium requirements in cattle depend on breed, age and parity, path physiological status, body condition score, gestation status, and lactation. During the periparturient period, the cows struggle to maintain calcium homeostasis, and if it is failed, the animal will experience hypocalcemia. As is known hypocalcemia as a predisposing factor for a number of reproductive disorders and infectious diseases. This study aims to investigate changes in blood calcium levels in Bali cattle during the periparturient period. The object of research is nine months pregnant Bali cattle and in good health, kept in cage in a horticultural farming environment. Three ml blood samples were taken through the jugular vein three times, namely in the last three weeks of gestation, on day parturition, and three weeks after calving. Blood samples were processed using the wet digestion method, and calcium concentrations were measured using the flame atomic absorption spectrometric method. The results showed an average blood calcium concentrations of 9.57±1.26 mg/dl, 4.11±0.63 mg/dl, and 8.32±1.23 mg/dl, respectively. Statistical analysis showed blood calcium levels during delivery experienced a very significant decrease. We find new evidence regarding the physiological data of Bali cattle, that at the time of parturition they experienced subclinical hypocalcemia. We concluded that there was a fluctuating change in blood calcium concentrations, where the normal value in the last three weeks of gestation experienced a significant decrease at the parturition and increased after three weeks of calving but has not yet reached the initial condition.
Efficacy of Polyherbal Oil Against Gastrointestinal Nematode in Kintamani Dogs I Made Merdana; Luh Made Sudimartini; Samsuri Samsuri; Putu Devi Jayanti
Journal of Veterinary and Animal Sciences Vol 4 No 2 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i02.p02

Abstract

Gastrointestinal helminth parasites in animals can be zoonotic-parasitic. This study aims to determine the efficacy of polyherbal oil formulations against intestinal worm parasites of Kintamani dogs. This study used 24 Kintamani Dogs aged 3-6 months which were kept by releasing them into the wild. The anthelmintic potency of polyherbal oil formulations was determined by measuring Eggs Per Gram of feces (EPG), using the Modified Stoll Dilution method before and seven days after treatment. P0 as control was given a placebo, while groups P1, P2, and P3 were given Polyherbal oil at doses of 300, 600, and 900 mg/kg body weight for three consecutive days. The results showed that there was a decrease in the EPG value sequentially P0, P1, P2, and P3 which was -4%, 23.1%, 66.7%, and 81.3%. Based on the identification of worm eggs, it was shown that the efficacy of polyherbal oil against Toxocara canis decreased EPG at P1, P2, and P3 by 26.09%, 73.33%, and 86.08%. Against Ancylostomata spp respectively at P1, P2 and P3 caused a decrease in EPG of 17.39%, 52.63%, and 72.0%. Against Strongyl spp worms respectively at P1, P2 and P3 caused a decrease in EPG by 16.67%, 58.82%, and 73.68%. Statistical analysis showed that polyherbal oil had a significant effect (P<0.05) on the decrease in EPG. We can conclude that the polyherbal oil formulation has vermicidal efficacy against intestinal worm parasites in Kintamani dog puppies.
Herbal Extract as An Antibacterial Against Gram Positive Bacteria Causing Dermatitis Complex I Nyoman Suartha; I GustiKetut Suarjana; Luh Made Sudimartini; I Made Merdana; I Made Dira Swantara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p07

Abstract

Study in order to evaluate the antibacterial effect of herbal extract from leaves of three different species of plant namely neem(Azadirachtaindica A. Juss), gotu-kola (Centellaasiatica), andsoursop (Annona muricata L) against bacteria causing dermatitis complex in dogs have been undertaken. The antibacterial activities of the herbal extract were evaluated using the agar diffusion test with extract at three different concentrations 5%, 10%, and 25%, respectively.Staphylococcus aureus was isolated from the dermatitis complex cases in dogs. The results showed various antibacterial activities of all the herbal extract at all concentration as indicated by the diameter of inhibition haloes produced around the orifice. The average inhibition haloes for each herbal extract concentration was 2.19± 0.53 mm; 4.32± 0.84 mm; 5.86± 0.79 mm, respectively. It can be concluded that herbal extract from the three plants is a potential antimicrobial against agents causing dermatitis complex.
6. In Vitro Insecticidal Activities of Ethanol Extract of Neem Leaves Against Heterodoxus Spiniger Lice In Dogs I Made Merdana; Monica Lewinsky; Ida Ayu Pasti Apsari
Jurnal Medika Veterinaria Vol 14, No 1 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i1.16203

Abstract

Heterodoxus spiniger is a chewing lice that have been reported to infest dogs and cats. This ectoparasite is haematophagous and as an intermediate host in transmission of cysticercoids and egg of nematode worms. This study aims to determine the in vitro insecticidal activity of ethanol extract of neem leaf (Azadirachta indica) against H. spiniger. A total of 250 H. spiniger were divided into five groups with five replications, namely: P0 as a negative control group given placebo, P1 as a positive control group given deltamethrin 0.5EC 0.05%, and groups P2, P3 and P4 were given ethanol extract of neem leaves with concentrations of 2.5%, 5% and 10%. In each test, 10 lice samples were put into a petridish with a cotton swab that had been sprayed with the solution of each treatment, then the lice were treated again with fine spray evenly. The results showed that the in vitro ethanol extract of neem leaves had a very significant effect (P0.01) on the mortality of H. spiniger. The best results are shown at 5% concentration which is capable of killing 100% of lice in 30 minutes. It can be concluded that the ethanol extract of neem leaf has insecticidal activity against H. spiniger.
Co-Authors Adri Yana Rambu Anawenju Afrizal Choirul Umam Agustina, I Putu Sudana Ali, Ahmad Muhammad Alviana Rizqiyah Utami Anak Agung Ayu Mirah Adi Anak Agung Gde Arjana Anak Agung Gde Arjana Anak Agung Gde Oka Dharmayudha Anak Agung Sagung Kendran Anastasia Bhala Ari Mogi, I Komang Arinata, I Ketut Teguh Ayu Prawitasari Citra Pratama Bina Ichsantya Darmawan, I Wayan Eka Dini Hilary Manullang Diyasa, I Gede Susrama Mas Domingas Pereira DWI SURYANTO Elsa Hidayati Elti Febilani Febrina Cicilia Br Ginting, Febrina Cicilia Firdaus, Ihsanul Franky Samuel Milenyano Chandra Fuady Muslih Gde Jasmara Muda Gea, Octo Berkat Gede Iwan Setiabudi Gede Yuda Darmadi Putra Genta Dhamara Adam Putranto Gusti Agung Rama Wiratama Putra Hariyadi, Hariyadi Hartati, Ruth Dwi Hendrawan, I Wayan Dika Wahyu I Dewa Made Nurja Sadhi I Gusti Agung Gede Putra Pemayun I Gusti Ayu Sri Darmayani I Gusti Ketut Suarjana I Gusti Ngurah Sudisma I Gusti Putu Tovan Mahottama I GustiKetut Suarjana I Kadek Ariyuda Prasetya I Kadek Parnayasa I Ketut Berata I Ketut Berata I Ketut Suada I Ketut Suada I Komang Ari Mogi I Komang Ari Mogi I Made Damriyasa I Made Dira Swantara I Made Dodi Gunawan I Made Dwinata I Made Kardena I Made Oka Riawan I Made Robi I NYOMAN ADI SURATMA I Nyoman Sarsana I Nyoman Suartha I Nyoman Sulabda I Nyoman Sulabda I Putu Agus Hendra Wibawa I Putu Agus Santika Putra I Putu Dwi Komala Putra I Putu Mangku Mariasa I Wayan Sudira I Wayan Wirata Ida Ayu Pasti Apsari Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Oka Winaya Ika Hartini Hutasoit, Ika Hartini Iwan Harjono Utama Jihadulhaq, Jihadulhaq Kadek Febriana Marta Putra Kadek Teguh Wirasastra Ketut Budiasa Kusuma, Putu Winatha Luh Ani Luh Gde Sri Surya Heryani Luh Made Sudimartini Luh Seri Ani Made Gede Adi Surya Saputra Mahardika, I Gusti Bagus Mahmud, Fathmah Monica Lewinsky Ngudiyono Ngudiyono Ni Kadek Eka Widiadnyani Ni Luh Eka Setiasih Ni Luh Watiniasih Ni Nyoman Kencanawati Ni Wayan Arya Utami, Ni Wayan Arya Ningsih, Adriati NYOMAN DITA PAHANG PUTRA, NYOMAN Nyoman Sumiati Pradipta Hendra Setiawan, Pradipta Hendra Putera, I Gusti Ngurah Dwipayana Putra, I Dewa Agung Made Wihanjana Putra, I Made Arya Udyana Putra, Putu Adi Guna Purwaka Putri, Ayu Chitra Adhitya Putu Devi Jayanti Putu Henrywaesa Sudipa Putu Suastika Rama Adi Putra, Rama Adi Sampurno, Ilham Ade Widya Samsuri , Samsuri Samsuri Samsuri Samsuri Samsuri Samsuri Samsuri Samsuri Saputra, I Dewa Ketut Ari Setyawati, Luh Gede Sewoyo, Palagan Senopati Sis wanto Siswanto, Riefqy Tepu Sri Darmayani, IGA ST Kholifah Nor Azizati Suci Nur Qurani Teja, Putu Tessa Hariys Septianda Theresia Ene Ujan, Katarina Kewa Yoga Eka Prasetyo Yoga Pratama Mambela Sarungallo