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Motility and Biofilm of Ralstonia syzygii subsp. celebesensis, the Causative Agent of Banana Blood Disease Saraswati, Vivian Thea; Subandiyah, Siti; McCann, Honour C.; Joko, Tri
Jurnal Perlindungan Tanaman Indonesia Vol 29, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.103868

Abstract

Blood disease in bananas is caused by Ralstonia syzygii subsp. celebesensis. This disease is a significant phytopathological concern, leading to deterioration of the plant's xylem tissue. Symptoms observed in infected plants typically begin from the shoot and banana inflorescence and progressively spread to other parts of the plant. The spread of these symptoms may result from the motility of R. syzygii subsp. celebesensis within the vascular system. This study aims to investigate the bacterial motility and biofilm formation ability, both contributing to the blockage of water and nutrient flow in the plant. The research methods included bacterial cultivation, molecular detection, motility assays (swimming and swarming tests), as well as biofilm and pellicle formation assay. The results indicated that R. syzygii subsp. celebesensis exhibited sliding motility but did not demonstrate swarming ability. Additionally, the bacterium was capable of forming both biofilm structures and pellicle layers. These findings provide insights into the role of motility types and colony structure formation in the virulence of R. syzygii subsp. celebesensis, potentially influencing the infection process in banana plants. Furthermore, this study's results are expected to contribute to developing improved disease management strategies.
Serum Cholinesterase Levels And Their Correlation With Body Composition Measured By Bioelectrical Impedance Analysis (BIA) Nasrokhatun Diniah, Bibit; Sulistiyani, Sulistiyani; Joko, Tri; Budiyono, Budiyono
Mulawarman International Conference on Tropical Public Health Vol. 1 No. 1 (2025): The 3rd MICTOPH
Publisher : Faculty of Public Health Mulawarman University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background : Pesticide exposure has harmful effects on farmers, especially spraying farmers. All of pesticide classes such as organophosphates, carbamates, pyrethroids and organochlorines can have a disruptive effect on metabolism and energy storage. One of the bioindicators that can be used to identify farmers exposed to pesticides is AChE. Abnormal AChE levels stimulate nicotinic and muscarinic receptors in various organs, causing a wide range of metabolic disorders including adipose tissue dysfunction, inflammation and changes in body composition. Objective : The aim of this study was to examine the correlation between long-term pesticide exposure and potential body composition alteration in vegetable farmers. Research Methods/ Implementation Methods : This was a cross-sectional study with 46 farmers who spraying pesticide in vegetables area. Subjects were determined by purposive sampling and must fulfil the inclusion and exclusion criteria. Indicator of pesticide exposure measured by cholinesterase serum and history of exposure, while parameters of body composition by bioelectrical impedance analysis (BIA) using body fat monitor. There were body weight, body mass index, resting metabolism rate, total, visceral and subcutaneous fat, skeletal muscle also body age. Results : Subjects with abnormal AChE were only 8.9%. AChE level had no correlation with all components of the exposure history, but had a significant correlation with body composition parameters (p<0.05), that was body weight, BMI, percent fat, percent visceral fat, RMR with strength of correlation is weak (R 0.31-0.34). Conclusion/Lesson Learned : Based on this study AChE may represent a good indicator of metabolic alterations in body composition and may occur due to lipid metabolism disruption.
Faktor-Faktor yang Menyebabkan Penyakit Infeksi Terhadap Stunting (Studi Kasus di Wilayah Kerja Puskesmas Tanjung Kabupaten Brebes) Fitria, Ida; Joko, Tri; Raharjo , Mursid
Jurnal Impresi Indonesia Vol. 4 No. 9 (2025): Indonesian Impression Journal (JII)
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/jii.v4i9.7003

Abstract

Stunting merupakan masalah kesehatan global yang berakar pada multifaktor, termasuk gizi, paparan infeksi, dan lingkungan WASH (water, sanitation, hygiene). Review ini bertujuan menganalisis hubungan kualitas air minum dan riwayat penyakit infeksi dengan kejadian stunting pada balita. Metode yang digunakan adalah systematic review dengan penelusuran Google Scholar dan PubMed (artikel berbahasa Indonesia/Inggris), menyaring studi observasional yang melaporkan paparan kualitas air (sumber, indikator mikrobiologi, praktik pengolahan/penyimpanan) dan/atau riwayat infeksi (diare, ISPA, cacingan) terhadap stunting (HAZ < –2 SD). Dari 16 artikel yang lolos seleksi, mayoritas menunjukkan bahwa sumber air tidak layak, kontaminasi mikrobiologis (mis. keberadaan E. coli), serta praktik pengolahan dan penyimpanan yang kurang aman berasosiasi dengan peluang stunting yang lebih tinggi; demikian pula frekuensi/kejadian infeksi berulang—terutama diare—secara konsisten berkaitan dengan pertumbuhan linear yang terhambat. Sebagian studi melaporkan temuan yang melemah setelah penyesuaian ganda (status sosioekonomi, asupan gizi), namun arah asosiasi tetap seragam. Keterbatasan utama meliputi dominasi desain potong lintang, heterogenitas pengukuran paparan dan luaran, potensi recall bias, serta penyesuaian faktor perancu yang belum memadai. Disimpulkan bahwa peningkatan kualitas air minum—melalui household water treatment, penyimpanan aman, dan perlindungan sumber—yang terintegrasi dengan pencegahan/penanganan infeksi (imunisasi, cuci tangan pakai sabun, sanitasi layak, deworming) dan intervensi gizi berpotensi menurunkan beban stunting; riset longitudinal dengan metrik kualitas air yang terstandar masih dibutuhkan untuk memperkuat kausalitas.
Association between serum cholinesterase levels and body composition in vegetable farmers assessed by bioelectrical impedance analysis Diniah, Bibit Nasrokhatun; Sulistiyani, Sulistiyani; Joko, Tri; Budiyono, Budiyono
AcTion: Aceh Nutrition Journal Vol 10, No 3 (2025): September
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v10i3.2363

Abstract

Exposure to all pesticide classes can have a disruptive effect on metabolism and energy storage. The bioindicator that can be used to identify farmers exposed to pesticides is acetylcholinesterase (AChE). AChE can be a good indicator for changes in body composition due to its associations with various metabolic and physiological parameters. Abnormal AChE levels stimulate nicotinic and muscarinic receptors in various organs, causing a wide range of metabolic disorders, including changes in the body composition. This study aimed to examine the correlation between long-term pesticide exposure and potential alterations in body composition. This research was conducted in Sagarahiang village, one of the highest vegetable producers in Kuningan, West Java onJuly-August 2024. And it was a cross-sectional study of 46 farmers who sprayed pesticides in vegetable areas and was determined by purposive sampling. Data collected through interview using questionnaires and some tools, like indicators of pesticide exposure were measured by cholinesterase serum and history of exposure, whereas parameters of body composition were measured by bioelectrical impedance analysis (BIA) using a body fat monitor with eight electrodes. Data analysis using the Pearson correlation test. The percentage of subjects with abnormal AChE activity was four persons (8,9%). AChE levels had no correlation with all components of the exposure history, but had a significant correlation with body composition parameters (p<0,05), body weight (p=0,027), Body Mass Index (BMI) (p=0,020), total fat (p=0,038), visceral fat (p=0,030), and resting metabolism (p=0,037) with a weak correlation (R 0,31-0,34). Based on this study, AChE is not strong enough to assess changes in body composition clinically.
Several Factors Responsible For Sick Building Syndrome in Urban Settings: Literature Review Rozi, Choirul; Tri Joko; Nurjazuli
Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Vol. 7 No. 7: JULY 2024 - Media Publikasi Promosi Kesehatan Indonesia (MPPKI)
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/mppki.v7i7.5376

Abstract

Introduction: Urban dwellers are more susceptible to the adverse effects of air pollution and climate change. Sick building syndrome (SBS) relates to health issues experienced by workers as a result of indoor activities, air pollution, and climate change. The incidence of SBS is strongly correlated with environmental factors both within and outside the workplace. Objective: To identify current factors associated with the incidence of sick building syndrome in urban areas Method: Review of the literature compiled in both English and Indonesian from the databases of PubMed, Scopus, and Google Scholar. The literature, which is available in full text publications, was collected during the period of the last five years, from 2019 to 2024. Results: Temperature, relative humidity, microbes, air pollution, psychological factors, light, and ventilation in a room or workplace were some of the variables that affect the incidence of sick building syndrome in urban settings. Conclusion: SBS was related to a number of factors, including temperature, relative humidity, ventilation, lighting, pathogens, and psychosocial factors. These elements may have an individual or combined effect on worker productivity and the development of SBS.
Environmental Health Risk Control in the Management of Infectious Medical Waste in Hospitals Using the FMEA Method Nasir, Muhammad; Joko, Tri; Budiyono, Budiyono
Indonesian Journal of Global Health Research Vol 7 No 4 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i4.6359

Abstract

Medical waste refers to the waste generated from medical activities within healthcare facilities, including hospitals. Other studies indicate that 15-25% of medical waste is toxic, infectious, or radioactive, while 75-85% is non-hazardous. Despite the smaller volume of medical waste compared to other waste types, its improper management can pose significant health and environmental risks. This research aims to investigate the control of environmental health risks in the handling of infectious medical waste at hospitals using the FMEA method. This study employed an observational research design using a cross-sectional approach. A mixed-methods approach was applied, combining both quantitative and qualitative analyses to assess environmental health risk management related to medical waste processing based on priority or critical risks at RSUP Persahabatan. The target population included individuals involved in both the handling and treatment stages of medical waste using an incinerator at the facility. The sample selection for the FMEA analysis employed purposive sampling with a quota sampling technique.The study utilized an environmental risk analysis framework based on the Failure Mode and Effect Analysis (FMEA) method. The critical Risk Priority Number (RPN) analysis revealed the following three primary environmental health risks: (1) The risk of contracting environmental health-based infectious diseases through the transmission of pathogenic microorganisms or hazardous materials, associated with 12 failure modes in the medical waste handling process. (2) The risk of ambient air (work environment) contamination, associated with 5 failure modes in the medical waste handling process. (3) The risk of contamination of facilities and rooms where medical waste is generated, associated with 4 failure modes related to the operation of the incinerator during the medical waste treatment process. Environmental health risks with a high potential to arise from failure modes during the medical waste management stage at RSUP Persahabatan, as indicated by critical Risk Priority Number (RPN) scores.
FAKTOR FAKTOR YANG MEMPENGARUHI PENERAPAN SISTEM MANAJEMEN LINGKUNGAN ISO 14001 Septanti, Elza Muthia; Joko, Tri; Setiani, Onny
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 8 No. 2 (2024): AGUSTUS 2024
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v8i2.30934

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Lingkungan menjadi prioritas internasional sejak Konferensi Lingkungan Stockholm 1972 dan KTT Bumi 1992. Setelah KTT Pembangunan Berkelanjutan Dunia (WSSD) 2002, berbagai mekanisme pengelolaan lingkungan telah didirikan. Isu lingkungan kini menjadi fokus perusahaan, terutama dalam industri yang berdampak besar pada ekologi. Polusi industri ini, bersama dengan polusi perkotaan dan penggunaan bahan kimia, mengancam kesehatan dan kesejahteraan manusia. Di Indonesia, kebijakan keberlanjutan mengharuskan perusahaan mengendalikan polusi dan memelihara fungsi ekologis, didukung oleh Undang-Undang No. 32 Tahun 2009 yang menuntut informasi valid tentang perlindungan lingkungan. Perusahaan diharuskan memenuhi standar kualitas lingkungan, yang mendorong adopsi langkah hijau seperti standar ISO-14001. Standar ini membantu perusahaan publik beroperasi ramah lingkungan dan mengurangi dampak perubahan iklim, serta meningkatkan nilai perusahaan dan daya saing. ISO-14001 diterapkan oleh 307.059 perusahaan secara global pada 2018, dengan 1.944 di Indonesia. Ini menunjukkan peran penting ISO-14001 dalam strategi industri hijau, mengurangi risiko pencemaran, dan mendukung pertumbuhan bisnis yang berkelanjutan dan bertanggung jawab. Implementasi efektif ISO-14001 memastikan kepatuhan terhadap standar tertinggi dalam menjaga keamanan dan kesehatan masyarakat, serta pelestarian lingkungan. Tujuan dari penelitian ini adalah untuk mengetahui faktor faktor yang mempengaruhi implementasi ISO 14001 di berbagai negara. Metode yang digunakan adalah metode literature review berdasarkan jurnal terkait dari tahun 2014 hingga 2024. Berdasarkan hasil tinjauan, ISO 14001 telah membantu dalam mengurangi emisi dan meningkatkan produktivitas serta efisiensi perusahaan.
Maize induced resistance against downy mildew mediated by benzoic acid increased photosynthesis rate and chlorophyl content Habibullah, Muhammad; Joko, Tri; Wibowo, Arif; Widiastuti, Ani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225255-261

Abstract

Maize downy mildew, caused by Peronosclerospora spp., is a major disease affecting maize cultivation in tropical regions, particularly in Indonesia. One potential control strategy is the induction of plant resistance. Previous studies have demonstrated that benzoic acid (BA), a non-fungicidal chemical compound, can induce maize resistance against downy mildew. Induced resistance mechanisms do not directly inhibit pathogen development but enhance the plant’s defensive response to infection. This study utilized various maize lines, ranging from susceptible to resistant, to evaluate the impact of BA-induced resistance on photosynthesis rate and chlorophyll content as the key agronomic parameters associated with the plant growth. The results showed that BA treatment did not significantly increase the photosynthetic rate and chlorophyll content in moderately resistant and resistant maize lines. However, in susceptible and moderately susceptible lines, BA treatment enhanced both parameters, indicating its role in strengthening plant resistance. These findings suggest that resistant maize lines do not respond to BA induction, whereas susceptible and moderately susceptible lines benefit significantly from its application. This provides new insight that BA can potentially induce resistance in susceptible maize plants against downy mildew.
IMPLEMENTASI MANAJEMEN ORGANISASI SISWA INTRA SEKOLAH SEBAGAI STRATEGI DALAM PENGEMBANGAN KEPEMIMPINAN SISWA SMP NEGERI 2 SUKADANA Joko, Tri
JURNAL LENTERA [PENDIDIKAN PUSAT PENELITIAN LPPM UM METRO] Vol 3, No 1 (2018): Jurnal Lentera Pendidikan Pusat Penelitian LPPM UM Metro
Publisher : LPPM UM Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/jlpp.v3i1.824

Abstract

Kandungan Logam Berat pada Residu Insinerator Limbah Medis dan Pengendalian Risiko Kesehatan Lingkungannya Nasir, Mohammad; Joko, Tri; Budiyono, Budiyono
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v10i4.58625

Abstract

Pengolahan limbah medis rumah sakit melalui insinerator masih menjadi tantangan di negara berkembang, termasuk Indonesia, karena dapat memengaruhi kesehatan dan lingkungan. Penelitian ini bertujuan untuk menganalisis kandungan logam berat dalam residu insinerator limbah medis, serta mengidentifikasi risiko kesehatan yang terkait. Metode yang digunakan adalah tinjauan sistematik terhadap 21 artikel yang relevan, yang mencakup analisis kandungan logam berat dalam bottom ash dan fly ash dari insinerator. Penemuan menunjukkan bahwa residu ini mengandung elemen toksik potensial (PTEs) seperti arsenik, kadmium, timbal, dan merkuri, dengan konsentrasi yang seringkali melebihi batas baku mutu yang ditetapkan. Pembuangan residu secara landfill berpotensi menyebabkan pencemaran lingkungan dan risiko kesehatan bagi masyarakat sekitar. Implikasi dari penelitian ini menekankan perlunya pengendalian yang lebih baik terhadap limbah insinerator, termasuk pengembangan teknik pengolahan yang ramah lingkungan dan peningkatan kesadaran bagi petugas. Penelitian ini juga merekomendasikan penguatan regulasi dan metode pemantauan untuk mengurangi dampak negatif dari limbah medis, serta perlunya studi lebih lanjut mengenai teknik ekstraksi logam berat yang efisien dan penerapan prinsip ekonomi sirkular dalam pengelolaan limbah medis.
Co-Authors - Suhartono Abdullah, Jilan Tsani Achmadi Priyatmojo Adi, Bayu Kusuma Aditya, Vanessa Rizky Agus Bintara Birawida Ahmad Zaerozi Ani Widiastuti, Ani Annisa Aulia Nurrohmah Anugrah Febrino Balwa Anwar, Wakhid Arif Wibowo Arlyna Budi Pustika Asma Afifah Astorina, Nikie Azmi Umi Anisyah Bariyadi Rifai Bella Arieza Andriyana Bibit Nasrokhatun Diniah Budi Waloyo Budiyono Budiyono Burhanuddin Thohir Candra Rulyana Charles foeh Foeh Choirul Luluk Fatimah Cornelius Saryadi Turnip Delima Kurnia Sari Devita Nur Aprilia Dwimartina, Fina Dyah Agustin Catur Putri Eka Luvita Sari Eko Handoyo Elisa Maharani Elzha Af’idatul Himmah Farida Sugiester S. Fianti andua Fitria, Ida Hanan Lanang Dangiran Hariyanti, Fika Harper, Stephen Haryudi Okta Sofiyanto Hidayanty, Healthy Ijaz ul haq Ilma, Hurin Nabila Aghnia Intan Aulia Putri Irawati, Roosdiana Jalaluddin Abdul Malek, Jalaluddin Abdul Jayawarsa, A.A. Ketut Karroghi, Silmi Aziza Lisa Okta Permatasari Maharani, Puti Maulida Khairunnisa McCann, Honour C. Mohammad Nasir, Mohammad Muhammad Addin Rizaldi Muhammad Habibullah Muhammad Nasir Mursid Rahardjo Mursid Raharjo Mustafiroh Kasanah Nabiha, Puteri Inandin Nabilah Nurhidayanti Nabilah Zahra Nasrokhatun Diniah, Bibit Ngazis, Muhammad Niki Astorina Yunita Dewanti Nikie Astorina Yunita Dewanti Nila Himayati Nilna Fasyya Salsabila Noor Zahrotul Muniroh Nur Endah Wahyuningsih Nur Fajria Safarina, Ismi Nurdiyanto, Miftakhul Nurjazuli Nurjazuli Nurjazuli Nurjazuli Nurjazuli Onny Setiani Pratiwi, Annisa Hasta Purnomo Purnomo Putri, Novi Hervianti Qorina Sabila Fa&#039;iza Raharjo , Mursid Rahman, Muhammad Auliya Rahmi Nur Alifiani Restu Andri Setiyanto Riska Triafryani Putri Rozi, Choirul Ruba, Yohanes Emanuel S. Russeng, Syamsiar Sahiledengle, Biniyam Salahuddin, Nurul Syahriani Santya Nareswari Saraswati, Vivian Thea Savitri Rachmawati Septanti, Elza Muthia Shofa Rakhmatika Shrestha, Akina Sinta Nugraheni Dewi Siti Nurkhayati Siti Subandiyah Slamet Wardoyo Suhartono Sukri Palutturi SULISTIYANI SULISTIYANI Sundari, Dini Suriah Suriah Suryanti Susamto Somowiyarjo Tati Suharti Triwidodo Arwiyanto Turnip, Cornelius Saryadi Ulfah Rahmawati Vinidia Pertiwi Wahiduddin Wahiduddin Wakhid Anwar Wardana, Lalu Muhammad Fikri Wibowo, Eko Prasetyo Wisanggeni, Gen Adi Woldesenbet, Biruk Yuni Pradilla Fitri, Yuni Pradilla Yusniar Hanani Danudianti Yusniar Hanani Darundiati