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Journal : Ovozoa: Journal of Animal Reproduction

Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) iqbal dwi warsito; Suherni Susilowati; Erma Safitri; Ragil Angga Prastiya; Maya Nurwartanti Yunita; Amung Logam Saputro
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.16-24

Abstract

This study aimed to evaluate the protective effects of α-tocopherol and zinc sulfate on oxidative stress parameters in ovarian granulosa cells of female Wistar rats (Rattus norvegicus) exposed to lead acetate (Pb(C₂H₃O₂)₂). A total of 25 rats were randomly divided into five groups: Control (C), lead exposure only (T0), α-tocopherol + lead (T1), zinc sulfate + lead (T2), and α-tocopherol + zinc sulfate + lead (T3). Treatments were administered orally for 21 days. Superoxide dismutase (SOD) levels were measured spectrophotometrically, and p53 expression was analyzed using immunohistochemistry. The results showed that SOD levels significantly decreased in group T0 compared to the control (p <0.05), while treatment with either α-tocopherol (T1), zinc sulfate (T2), or their combination (T3) significantly improved SOD levels compared to T0. Moreover, p53 expression was markedly elevated in T0, indicating enhanced oxidative stress and potential apoptosis, whereas all antioxidant-treated groups showed reduced p53 expression, with T3 demonstrating values comparable to the control group. In conclusion, the combination of α-tocopherol and zinc sulfate provided a synergistic antioxidant effect, effectively enhancing SOD levels and suppressing p53 expression in granulosa cells of lead-exposed rats. This suggested a promising therapeutic potential of these compounds in mitigating lead-induced ovarian toxicity.
Co-Authors Agustono, Bodhi Al arif, Mohammad Anam Al-Zuhroh, Tisya Yumn Amung Logam Saputro Amung Logam Saputro Arimbi Ashari, Lukman Baroroh, Diana Nova Cahya, Nedriana Cahya, Rizka Wulan Chozin, Aminullah Alhady Chusniati, Sri Dayanti, Efin Windi Erma Safitri ERMA SAFITRI Faisal Fikri Fania Aulia Rahma Farodina Azka Zhafira Fatimatus Zahro, Fatimatus Fiqih, Adam Fahmi Hamid, Iwan Sharial Hani Plumeriastuti Harelas, Genoveva Callista Harijani, Nenny Hayati, Laila Nur Herdiansyah, Akbar Dimas Hidajati, Nove IMAM MUSTOFA Indaka Rachmach Choirunnisa iqbal dwi warsito Ira Sari Yudaniayanti Ivanalee, Angela Swasti Iwan Sahrial Hamid Kuncoro Puguh Santoso Kuncorojakti, Suryo Kusuma, Agung Jati Kusumastuti, Imbi Lake, Werenfridus Kono Megawati, Neneng Ismi Moch. Ilham Riza Fahlefi Muhammad Thohawi Elziyad Purnama Murjianingsih, Fitriani Mustofa Helmi Effendi Nina Amalia, Nina Ningrum, Indah Puspita Nove Hidajati Novitasari, Seline Putri Nusdianto Triakoso Praja, Ratih Novita Prameswari, Febriana Hawa Prameswari, Ratna Ajeng Prastiya, Ragil Angga Pratika, Selva Primarizky, Hardany Putri, Diana Novita Putrianirma, Rizki Rahmadani, Aurel Aulia Rahmahani, Jola Rahmawati, Anlisia Salahuddin, Muhammad Soleh Salsabila Putri, Elma Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Shelly Oktania Nurvita Sarudji, Suryanie Sekarsari, Dara Ayu Pramesti Shabrina Fauzia Prayoga Soeharsono Soeharsono Solikhah, Tridiganita Intan Subagyo, Safira Fauziyyah Suherni Susilowati Suwarno Suwarno Trilas Sardjito Tyasningsih, Wiwiek Wardana, Prasvita Rosa Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Wijaya, Achmad Arby Wijaya, Nanik Sianita Wilujeng, Estiary Yeni Dhamayanti Yudhana, Aditya Yustinasari, Lita Rakhma Zhafira, Farodina Azka Zulianti, Brillia