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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Rekayasa Proses JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia OSEATEK DIPOIPTEKS: Jurnal Ilmiah Mahasiswa Undip Jurnal Matematika & Sains Prosiding Indonesian Journal of Pharmaceutical Science and Technology Proceeding Seminar LPPM UMP Tahun 2014 Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Marine Research in Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Rekayasa Proses Indonesian Journal of Energy Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding University Research Colloquium Prosiding Konferensi Nasional Penelitian Matematika dan Pembelajarannya
Ocky Karna Radjasa
Badan Riset Dan Inovasi Nasional
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Seleksi Isolat Bakteri Amilolitik dari Rhizosfer Canna edulis, Kerr. untuk Produksi Poli Hidroksi Alkanoat dari Limbah Cair Tapioka Nurhayati Nurhayati; Ocky Karna Radjasa; Irfan Dwidya Prijambada
Jurnal Rekayasa Proses Vol 11, No 2 (2017)
Publisher : Departemen Teknik Kimia Fakultas Teknik Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jrekpros.33194

Abstract

Petrochemical-based plastic waste accumulated in landfills have been posing serious threat to the environment as this kind of plastics are non-biodegradable. Replacing petrochemical-based plastics with biodegradable plastics constitutes a challenging solution both in terms of mechanical design of the process and most importantly the availability of powerful local microorganism for the process. Therefore, the current study was searching for appropriate local microorganisms for poly hydroxyl alkanoate (PHA) production from starch waste, which was considered as one of cheap carbon sources. Waste water of cassava industry is a good resource of such starch waste water. The microbes were isolated from Canna edulis, Kerr. rhizosphere from Cangkringan.  The expected isolates were the bacteria enable the coupling of carbon catabolic pathways with PHA anabolic pathways. It was found that ten isolates were able to use waste water of cassava flour industry as carbon source. The PHA quantitative analysis by spectrophotometer showed that the isolate of Bacillus sp. C8 produced the highest PHA of 2,095 g/L. Further FTIR analysis showed specific bands near 1363,67 cm-1, 1641,42 cm-1, 2929,87 cm-1, 3408,22 cm-1 wavelengths which revealed the presence of CH3, ester carbonyl group (C=O), C-H and terminal OH group of PHA. ABSTRAKAkumulasi sampah plastik berbasis petrokimia di tempat pembuangan sampah mengganggu lingkungan karena plastik sifatnya tidak mudah didegradasi secara biologi dan sangat tahan di lingkungan. Penggantian plastik yang berasal dari bahan petrokimia dengan bahan plastik yang mudah terdegradasi secara biologi merupakan tantangan tersendiri, baik dari sisi perancangan proses maupun ketersediaan mikrobia lokal yang sesuai untuk proses tersebut. Oleh karena itu, penelitian ini dilakukan sebagai upaya untuk mendapatkan bakteri lokal penghasil PHA yang mampu mengkonsumsi substrat berupa pati. Substrat pati dipilih karena ketersediaan limbat industri tapioka sebagai bahan baku potensial dan murah untuk produksi PHA. Bakteri amilolitik untuk produksi PHA telah berhasil diisolasi dari rhizosfer Canna edulis, Kerr. di Cangkringan, Sleman, Yogyakarta, Indonesia. Bakteri yang diisolasi merupakan bakteri dengan kemampuan memproduksi PHA dan memiliki kemampuan melakukan rangkaian reaksi pada limbah cair industri tapioka dan rangkaian reaksi pembentukan PHA. Telah berhasil didapatkan 10 bakteri yang memiliki aktivitas amilolitik dan dapat menghasilkan PHA  menggunakan limbah cair industri tapioka. Analisis kuantitatif PHA menggunakan spektrofotometer menunjukkan bahwa isolat Bacillus sp. C8 menghasilkan PHA paling tinggi sebesar 2,095 mg/mL. Analisis hasil metabolism isolat C8 menggunakan FTIR memperlihatkan puncak spesifik 1363,67 cm-1, 1641,42 cm-1, 2929,87 cm-1, 3408,22 cm-1 adalah verifikasi adanya CH3, C=O, C-H dan OH dari PHA.
Identification of Sponge-Associated Bacteria with Antibacterial Property against Staphylococcus aureus based on Molecular Approach Ocky Karna Radjasa
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.282

Abstract

Staphylococcus aureus is among human pathogens which are known to be resistant to almost clinically significant antibiotics that causes a crisis, in the treatment and management of infectious disease, and also presents a clear danger to the future of public health. Recently, invertebrate-associated microorganisms have become the target of the search for marine microbial natural products. Sponge-associated microorganisms are one of the most interesting natural product sources because they produce bioactive natural products including polyketides and nonribosomal peptides. Molecular approach based on 16S rDNA has been very successful in the identification and the search of secondary metabolite-producing microorganisms, particularly by using specific degenerated primers, for the detection of Polyketide Synthases (PKS) and Nonribosomal Peptide Synthetases (NRPS) gene fragments that are essential for the biosynthesis of bioactive products. Four bacterial isolates from sponge Dysidea sp. were found to inhibit the growth of S. aureus. However, only the best two isolates were selected for further identification and screening for the presence of either PKS or NRPS. The results revealed that both isolates BSP5.11 and BSP11.7 have 99% homology with Alpha proteobacterium. PCR analysis of PKS and NRPS gene fragments showed that they both amplified the NRPS but not the PKS gene fragments.
Optimasi Substrat Dan Pemilihan Co-Substrat Media Kultur Yang Sesuai Terhadap Pertumbuhan Dan Aktivitas Protease Ektraseluler Bakteri Bacillus Firmus Dari Perairan Nusa Lembongan Bali, Indonesia Muhammad Zainuddin; Delianis Pringgenies; Ocky Karna Radjasa; Haeruddin Haeruddin; Aninditia Sabdaningsih; Vivi Endar Herawati
Journal of Marine Research Vol 11, No 2 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i2.33278

Abstract

Udang merupakan sumber protein hewani yang banyak diminati masyarakat karena citarasa enak dan bergizi. Udang merupakan komoditas perikanan ekspor. Upaya pemenuhan kebutuhan ekspor udang saat ini telah dilakukan budidaya intensif. Budidaya intensif telah terjadi pemberian pakan yang berlebih sehingga terbentuk limbah organik di dasar tambak. Limbah pakan mengakibatkan terbentuknya senyawa nitrit dan amoniak yang toksik bagi udang. Komponen utama limbah ini adalah protein. Protein harus di dekomposisi menjadi asam amino oleh bakteri proteolitik. Salah satu bakteri yang memiliki aktivitas proteolitik adalah bakteri Bacillus Firmus dari simbion sponge Chalinula Pseudomolitba. Tujuan penelitian adalah melakukan optimasi substrat dan pemilihan co-substrat media kultur yang sesuai terhadap pertumbuhan dan aktivitas protease ektraseluler Bacillus Firmus. Penelitian menggunakan metode eksperimen laboratoris. Penelitian terdiri dari uji optimasi substrat, pemilihan Co-substrat C dan N. Hasil penelitian menunjukkan bahwa bakteri Bacillus firmus memiliki laju pertumbuah dan aktivitas protease terbaik pada media kultur dengan subtrat susu skim 1% dengan nilai 0,110 c/jamdan 12,566 c IU/ml. Sumber C terbaik adalah glukosa dengan nilai 0,124 c/jamdan 16,094 cIU/ml. Sumber N terbaik adalah amonium nitrat dengan nilai sebesar 0,137 c/jamdan 19,046 c IU/ml. Shrimp is a source of protein and have good taste. Shrimp is an export commodity. Shrimp cultivation is developing to be intensive. Intensive cultivation uses large amounts of feed. not all of the feed is eaten. leftover feed becomes waste. Feed waste produces nitrite and ammonia. Ammonia is toxic. Feed waste must be cleaned. Decomposition process by proteolytic bacteria. Bacillus Firmus is a proteolytic bacterium from the symbiont sponge Chalinula Pseudomolitba. The research aims to optimize the substrate and select the co-substrate. The study used a laboratory experimental method. The research consisted of a substrate optimization test, selection of Co-substrate C and N. The results showed that Bacillus Firmus had the best growth rate and protease activity on 1% skimmed milk substrate with values of 0.110 c/hour and 12,566 c IU/ml. The best source of C is glucose with a value of 0.124 c/hour and 16,094 c IU/ml. The best source of N as ammonium nitrate with values of 0.137 c/hour and 19,046 c IU/ml.
Diversity and Antifungal Activity of Actinomycetes Symbiont Hard Coral Mucus of Genera Goniopora and Porites Riyanti,; Nurkhasanah, Wulan; Radjasa, Ocky Karna
Makara Journal of Science Vol. 20, No. 4
Publisher : UI Scholars Hub

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Abstract

Screening new bioactive compounds from marine actinomycete organisms associated with corals (Goniopora and Porites) can be an alternative method to discover the natural antifungal compounds. This study aims to determine the density and diversity of actinomycete symbionts based on repetitive sequence-based-polymerase chain reactions (rep-PCR) and to discern the ability of antifungal activity of isolates symbiotic with hard coral mucus by using a pour plate method. A total of 143 isolates were obtained from the hard coral mucus of genera Goniopora and Porites. High genetic diversity was observed among the isolates. Ten isolates with different morphological characteristics were selected to extract its secondary metabolites and then followed by an antifungal test. The isolate with the code of SCAS324 was the one with the antifungal activity, marked by the formation of a very strong inhibition zone of 54.7±0.4 mm toward Aspergillus flavus and 49.2±2.7 mm toward Candida albicans. Antifungal screening showed that the antifungal activity of the isolate SCAS324 was three times as effective as the commercial antifungal.
Prediksi Aspek Evolusi Xantofil pada Exiguobacterium Sp. yang Berasosiasi dengan Halimeda Macroloba, Hasil Isolasi dari Perairan Pulau Menjangan Kecil, Karimunjawa Wiwik Astuti; Ocky Karna Radjasa; Ferry Fredy Karwur; Ferdy Semuel Rondonuwu
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.617 KB)

Abstract

Xantofil merupakan oksikarotenoid yang memiliki kemampuan sebagai fotoprotektor, sehingga merupakan senyawa antioksidan yang potensial. Sebarannya yang luas dari tumbuhan tingkat tinggi hingga organisme tingkat rendah, termasuk pada bakteri, menyebabkan xantofil mudah didapat, terlebih lagi dengan sifat kepolarannya, membuat pigmen oksikarotenoid ini relatif mudahuntuk diisolasi dari senyawa karoten lain. 3 jenis xantofil, yaitu dinosantin, P457, dan diadinokrom yang teridentifikasi dari Exiguobacterium sp. MK_HM, bakteri yang berasosiasi dengan karang Halimeda macroloba, hasil isolasi dari perairan Pulau Menjangan Kecil, Karimunjawa, memiliki kesamaan dengan xantofil yang dihasilkan oleh beberapa Dinoflagellata. Hal ini dimungkinkan karena efek asosiasi sekunder yang dilakukan inangnya, Halimeda macroloba dengan Dinoflagellata tertentu.
REFORMASI KEBIJAKAN RISET DALAM MENDUKUNG KINERJA PERGURUAN TINGGI Ocky Karna Radjasa
Prosiding University Research Colloquium Proceeding of The 8th University Research Colloquium 2018: Bidang Sosial Ekonomi dan Psikologi
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

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Korelasi Konsentrasi Mikroplastik dengan Material Padatan Tersuspensi (MPT) di Perairan Delta Sungai Bodri, Kendal, Jawa Tengah Sri Yulina Wulandari; Bambang Yulianto; Ocky Karna Radjasa; Dwi Haryo Ismunarti; Sri Sedjati
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.16121

Abstract

The Bodri river flow in Kendal Regency is a transportation route for sedimentary materials, waste disposal or garbage resulting from human activities on land to sea waters. One type of waste that is often found is plastic packaging. Until now, the use of plastic products is still high in society. In the environment, plastic waste (including that used for packaging) is difficult to decompose or degrade, but only fragmented into smaller sizes called microplastics. Microplastics (particle size < 5 mm) are widely distributed throughout aquatic ecosystems. Microplastics are one of the pollutants that can affect the food cycle for organisms in coastal and marine areas. This study was aimed to determine the relationship between the concentration of microplastics and suspended solids (MPT) at high tide and low tide. A total of 16 water samples were taken from eight (8) purposively determined stations. Sampling was carried out in July 2019 (dry season) during high and low tides. The research method used was descriptive quantitative. The concentration of microplastics was determined by the method recommended by NOAA, while the determination of the concentration of MPT was carried out by the gravimetric method. The results showed that the average concentration of microplastics at high tide was 2.207 mg/L compared to 2.615 mg/L at low tide. The average MPT concentration at high tide is 193 mg/L lower than at low tide, which was 419 mg/L. It could be said that the high mean concentration of microplastic and MPT at low tide indicated that more microplastic and MPT sourced from the mainland. By the statistical test using the Pearson correlation method, the relationship between microplastics and MPT was in the weak category. MPT affected the presence of microplastics only by 3.19% (with a coefficient of determination of 0.0319 and a p-value of 0.508). The presence of microplastics was not only caused by the presence of MPT, but was mostly thought to be influenced by other factors. Aliran sungai Bodri di Kabupaten Kendal menjadi jalur transportasi bahan sedimen, buangan limbah atau sampah hasil dari aktivitas manusia di daratan menuju ke perairan laut. Salah satu jenis buangan sampah yang banyak dijumpai adalah plastik kemasan. Sampai sekarang, penggunaan produk plastik masih tinggi di masyarakat. Di lingkungan, sampah plastik (termasuk yang digunakan sebagai kemasan) sulit terurai, tetapi hanya terfragmentasi menjadi ukuran yang lebih kecil yang disebut mikroplastik. Mikroplastik (ukuran partikel < 5 mm) tersebar luas di seluruh ekosistem perairan. Mikroplastik menjadi salah polutan yang dapat mempengaruhi siklus makanan bagi organisme di wilayah pesisir dan laut. Penelitian ini bertujuan untuk mengetahui hubungan antara konsentrasi mikroplastik dengan material padatan tersuspensi (MPT) pada saat pasang dan surut. Sebanyak 16 sampel air diambil dari 8 stasiun yang ditentukan secara purposif. Pengambilan sampel dilakukan pada bulan Juli 2019 (musim kemarau) saat pasang dan surut. Metode penelitian yang dilakukan adalah deskriptif kuantitatif. Konsentrasi mikroplastik ditentukan dengan metode yang direkomendasikan oleh NOAA, sedangkan penentuan konsentrasi MPT dilakukan dengan metode gravimetri. Hasil penelitian menunjukkan, bahwa rerata konsentrasi mikroplastik saat pasang lebih rendah yaitu sebesar 2,207 mg/L dibandingkan saat surut yaitu sebesar 2,615 mg/L. Rerata konsentrasi MPT saat pasang lebih rendah yaitu sebesar 193 mg/L dibandingkan saat surut yaitu sebesar 419 mg/L. Dapat dikatakan bahwa tingginya nilai rerata konsentrasi mikroplastik dan MPT pada saat surut mengindikasikan kalau mikroplastik dan MPT lebih banyak berasal dari daratan. Dari uji statistik dengan metode korelasi Pearson, hubungan antara mikroplastik dengan MPT termasuk kategori lemah. MPT mempengaruhi keberadaan mikroplastik hanya sebesar 3,19% (dengan koefisien determinasi sebesar 0,0319 dan p-value 0,508). Keberadaan mikroplastik tidak hanya disebabkan oleh adanya MPT, tetapi sebagian besar diduga dipengaruhi oleh faktor-faktor lain.
REFORMASI KEBIJAKAN RISET DALAM MENDUKUNG KINERJA PERGURUAN TINGGI Ocky Karna Radjasa
Prosiding University Research Colloquium Proceeding of The 8th University Research Colloquium 2018: Bidang Sosial Ekonomi dan Psikologi
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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The Community Structure of Phytoplankton in Seagrass Ecosystem and its Relationship with Environmental Characterstics Gede Iwan Setiabudi; Dietriech G. Bengen; Hefni Effendi; Ocky Karna Radjasa
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6549

Abstract

The aimed of this study was to determine  the plankton communities and its relationship with the chemical and physical condition in seagrass ecosystem at Pegametan Bay. The composition and abundance of plankton were observed in the sea water underneath the surface and were identified based on the guideline of Illustration of the Marine Plankton of Japan. The water quality was measured in situ using WQC HI 9829. The water sample was measured using closed reflux spectrometry for COD, TOC analyzer for DOC and APHA 2102 (4500) method for Nt and Pt. There are 27 species of plankton identified, which can be classified into three groups. Diatom group consists of 18 species with a 74.56% abundance. The non-litoral group consists of 6 species with a 23.35% abundance. Moreover, dinoflagellate group consist of 3 species with a 2.09% abundance. An abundance of plankton greater than 104 cell.L-1 was found in diatome group (Nitzschia sp., Thalassiosira sp., Chaetoceros sp., Flagillaria sp., Thalassiothrix sp., and Melosira sp.) and non-litoral group (Oscillatoria sp. and Spirogyra sp.). The abundance of those species indicated the algae bloom phenomenon. Dinophysis sp. was also identified, which was harmful algal blooms.How to CiteSetiabudi, G. I., Bengen, D. G., Effendi, H., Radjasa, O. K. (2016). The Community Structure of Phytoplankton in Seagrass Ecosystem and its Relationship with Environmental Characterstics. Biosaintifika: Journal of Biology Biology Education, 8(3), 257-269.
Kebijakan Riset dan Pengabdian Masyarakat Radjasa, Ocky Karna
Prosiding Konferensi Nasional Penelitian Matematika dan Pembelajarannya 2016: Prosiding Konferensi Nasional Penelitian Matematika dan Pembelajarannya
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Hilirisasi hasil-hasil penelitian melalui kegiatan-kegiatan pengabdian kepada masyarakat•Skim baru program pengabdian kepada masyarakat berbasis institusi: Iptek bagi Desa Mitra (IBDM)•Skim Baru: Iptek bagi Produk Unggulan Daerah(IbPUD)•Rencana Induk Pengabdian Masyarakat (RIPM)•Pemetaan Kinerja PT berbasis Pengabdian Masyarakat•Kajian kredit poin pengabdian dalam Angka Kredit
Co-Authors A. Sabdono ADI SANTOSO Agung Dhamar Syakti Agus Hardiyanto Agus Sabdono Agus Sabdono Agus Trianto Ali Ridlo Ambariyanto , Aninditia Sabdaningsih Antonius Budi Susanto Awang Surya Wiguna Awang Surya Wiguna, Awang Surya Bahry, Muhammad Syaifudien Bambang Yulianto Bayu Munandar Bintang Marhaeni Boedi Hendrarto Busaeri, Nundang Delianis Pringgenies Delianis Pringgenies Desy Wulan Triningsih Dietrich G Bengen Dietriech G. Bengen Dietriech Geoffrey Bengen Duhita Sinidhikaraning Kencana Dwi Haryo Ismunarti Elena Zocchi Endang Sri Lestari Endang Supriyantini Endang Supriyantini Fauziah Shahul Hamid Fera Nur Idawati Sahara Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu Ferdy Semuel Rondonuwu Ferry Fredy Karwur Gede Iwan Setiabudi Gede Iwan Setiabudi Haeruddin Haeruddin Handung Nuryadi HANS- PETER GROSSART Hariyadi, Putut Hefni Effendi Hendi Perdian Yunianto Hendi Perdian Yunianto Herawati Sudoyo Hidetoshi Urakawa Hidetoshi Urakawal Ika Wulan Santi Ika Wulan Santi Iqna Kamila Abfa Irfan Dwidya Prijambada Isai Yusidharta Ita Riniatsih Ita Widowati JAKA WIDADA JOEDORO SOEDARSONO Johannes Hutabarat Kouichi Ohwada Kouichi Ohwadai Kumiko Kita-Tsukamoto Kumiko Kita-Tsukamotoz Leenawaty Limantara Lia Kusmita Lia Kusmita Mada Triandala Sibero Mada Triandala Sibero McCauley, Erin Meinhard Simon Meris Rahmawati Meutia Samira Ismet Miftahuddin M Khoeri Miftahuddin Majid Khoeri Misbakul Munir Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Mujizat Kawaroe Mukti K. Diana Putri Mutiara, Frista Nestin E.W. Ningrum Ni Komang Tri Utami Norma Afiati Nuning Vita Hidayati Nurhayati Nurhayati Nurkhasanah, Wulan Nurmanita Rismaningsih Nurul Hiron, Nurul Nurul Ria Arlita Paulus Damar Bayu Murti Pinandoyo Pinandoyo Prastyo Abi Widyananto Putut Har Riyadi R. Riyanti Rachmat Afriyanto Richardus F Kaswadji Rismaningsih, Nurmanita Riyanti Riyanti . Riyanti Riyanti Riyanti Riyanti Riyanti, Rory Anthony Hutagalung Rr Citra Permata Rr Citra Permata Rr. Citra Permata Rr. Citra Permata Rudhi Pribadi Rudiger Stöhr S arjito S. Sulistiyani Saefuddin Aziz Sahala Hutabarat Sakti Imam Muchlisin Sakti Imam Muchlissin Sakti Imam Muchlissin Sarjito - Sekar Widyaningsih Septhy Kusuma Radjasa Seto Windarto Slamet B Prayitno slamet budi prayitno Slamet Budi Prayitno Sri Achadi Nugraheni Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Suharsono . Suhartono, Suhartono Supriharyono ,, Supriharyono Suyono ,, Suyono Suyono Suyono Tedi Septiadi Tonny Bachtiar TONNY BACHTIAR Tonny Bachtiar Tony Bachtiar Tony Bachtiar TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Uswatun Khasanah Vita Meylani Vivi Endar Herawati VIVI ENDAR HERAWATI Windarto, Seto Wiratno Wiratno Wismayanti, Gita Wittriansyah, Khoeruddin Wiwik Astuti Wiwik Astuti Wulan Nurkhasanah YS Darmanto Yustin Widyastuti