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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Rekayasa Proses JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia OSEATEK DIPOIPTEKS: Jurnal Ilmiah Mahasiswa Undip Jurnal Matematika & Sains Prosiding Indonesian Journal of Pharmaceutical Science and Technology Proceeding Seminar LPPM UMP Tahun 2014 Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Marine Research in Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Rekayasa Proses Indonesian Journal of Energy Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding University Research Colloquium Prosiding Konferensi Nasional Penelitian Matematika dan Pembelajarannya

Ocky Karna Radjasa

Badan Riset Dan Inovasi Nasional

Author-ID : 295934

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Other

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ANTI-BACTERIAL PROPERTY OF A CORAL-ASSOCIATED BACTERIUM Bacillus sp. AGAINST CORAL PATHOGENIC BBD (BLACK BAND DISEASE) Agus Sabdono; Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 9, No 3 (2006): Volume 9, Number 3, Year 2006
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Marine organisms such as corals are frequently colonized by bacteria that may be pathogenicto them. One of the means by which they are able to combat microbial attack is by chemical defense. Anumber of metabolites obtained from algae and invertebrates may be produced by associatedmicroorganisms. The purpose of study was to isolate and characterize of coral-associated bacteriahaving antibacterial potency against BBD coral disease. A coral-associated bacterium, KM2 isolate,was successfully screened for antibacteria production against indigenous BBD pathogenic bacteriabased on PCR amplification of the non-ribosomal peptide synthetase gene and was identified as closelyrelated to Bacilus sp based on its 16S rDNA.. KM2 strain was found to inhibit the growth of coralpathogenic BBD bacteria tested Myroides odoratimimus strain BBD1, Bacillus algicola Strain BBD2and Marine Alcaligenes bacterium Strain BBD3. This bacterium was found to inhibit the growth of allthose BBD coral pathogenic bacteria.

Identification of Carotenoids in Halimeda macroloba Reef Associated Bacteria Wiwik Astuti; Ocky Karna Radjasa; Ferry Fredy Karwur; Ferdy S. Rondonuwu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Several carotenoid pigments which are produced by sea microorganisms are shown to function as antimicrobe compounds and potential antioxidants. Coral reefs with high levels of diverse biota facilitate Halimeda as a primary component and producer to have associated bacteria that produce relatively unknown metabolic compounds. This research attempts to isolate bacteria that are associated with Halimeda macroloba, identify it, as well as analyze the pigment content produced. A yellow-orange bacteria colony is successfully isolated and given the temporary name MK_HM. This bacteria is in the shape of circular (oval) rods and are gram-variable bacteria. Based on the sequencing analytical results, Blast homology, as well as a phylogenetic analysis, it shows that the bacteria have a relationship with Exiguobacterium aestuarii TF-16 at 94%, so that it can be strongly predicted that the MK_HM strain is a new species. This pigment analysis, which is conducted on the Exiguobacterium sp. MK_HM bacteria extract, reveals that this strain produces carotenoid pigments of diadinochrome, semi-α-carotenone, dinoxanthin, and P457. Keywords: Exiguobacterium, carotenoid, diadinochrome, semi-α-carotenone, dinoxanthin, and P457.

Isolasi dan Identifikasi Bakteri Pendegradasi Koprostanol dari Lingkungan Sungai, Muara, dan Perairan Pantai Banjir Kanal Timur Semarang pada Monsun Timur Misbakul Munir; Norma Afiati; Ocky Karna Radjasa; Agus Sabdono; Tonny Bachtiar
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 2 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Aktifitas manusia yang terus meningkat di wilayah pesisir, telah menyebabkan terjadinya peningkatan tekanan lingkungan yang berasal dari berbagai macam sumber pencemaran, antara lain limbah industri, pertanian, transportasi, dan limbah domestik. Salah satu sumber pencemar yang cukup dominan di lingkungan perairan pantai adalah pencemaran akibat limbah domestik. Selama ini yang dipakai untuk mengetahui pencemaran lingkungan oleh limbah domestik adalah menggunakan indikator biologi yaitu bakteri coliform. Namunpenggunaan bakteri coliform sebagai indikator pencemaran limbah domestik mempunyai permasalahan antara lain tidak terdeteksinya bakteri coliform tersebut pada perairan pantai, sementara diduga kuat bahwa perairan tersebut tercemar oleh limbah domestik termasuk feces. Oleh karena itu indikator alternatif sangat diperlukan. Salah satu indikator alternatif pencemaran limbah domestik adalah koprostanol, yang mempunyai sifat cukup konservatif, dapat dikuntitatifkan dan dapat dihubungkan dengan sumber pencemar yang spesifik. Namun perlu diingat bahwa di alam, koprostanol mengalami proses degradasi oleh bakteri, sehingga perlu dilakukan penelitian mengenai isolasi dan identifikasi bakteri pendegradasi koprostanol pada lingkungan sungai, muara, dan perairan pantai Banjir Kanal Timur Semarang pada monsun timur. Penelitian dilakukan pada bulan Juli s/d Agustus 2003 pada lingkungan sungai, muara, dan perairan pantai Banjir Kanal TimurSemarang. Isolasi bakteri dari sampel air dan sedimen dilakukan di Laboratorium Mikrobiogenetika, FMIPA Jurusan Biologi UNDIP Semarang dan identifikasi bakteri pendegradasi koprostanol terseleksi dilakukan diLaboratorium Hama dan Penyakit Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara. Hasil penelitian diperoleh bahwa bakteri pendegradasi koprostanol yang berasal dari lokasi Banjir Kanal TimurSemarang pada berbagai variasi lingkungan dan habitat diperoleh 69 isolat (83,13%) dari 83 isolat yang diuji. Sedangkan hasil identifikasi terhadap 6 isolat terbaik diperoleh 3 (tiga) genus bakteri mampumendegradasi koprostanol, yaitu Achromobacter, Bacillus, dan Branhamella. Dari 3 genus bakteri pendegradasi koprostanol yang didapatkan, ada 2 (dua) genus yang selama ini belum dilaporkan, yaitu genus Achromobacter, dan Branhamella.Kata kunci : Koprostanol, Limbah Domestik, Indikator Pencemar, Isolasi, Identifikasi, BakteriIncreasing human activities in coastal areas have caused an increase of environmental pressures that come from various sources such as industrial disposal, agriculture, transportation, and domestic wastes. One of dominant sources in coastal waters is contamination by domestic wastes. So far to determine environmental contamination by domestic waste is by using biological indicator, coliform bacteria. However the use ofcoliform bacteria have problems for example, they cannot be detected in coastal waters contaminated by domestic waste including faeces. Therefore, an indicator alternative is urgently needed. Alternative indicatorof domestic waste contamination is coprostanol, which is conservative, easy to quantity and related to specific pollutant source. It is important to note coprostanol is naturally degraded by indigenous bacteria.Therefore it is necessary to conduct a study on isolation and identification of coprostanol-degrading bacteria in river, estuarine, and coastal environments of Banjir Kanal Timur Semarang during dry season. The research had been carried out from July to August 2003 at environmental of river, estuarine, and coastal of Banjir Kanal Timur Semarang. Isolation of bacteria from water and sediment samples were conducted atMicrobiogenetics Laboratory, Faculty of Mathematics and Natural Sciences Diponegoro University, meanwhile identificaton of coprostanol-degrading bacteria selected was conducted by at Pest and Diseases Laboratory,Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara. The result showed that coprostanol-degrading bacteria obtained from Banjir Kanal Timur Semarang at various environments and habitat were 69 bacterial isolates (83,13%) from 83 isolat tested. Identification revealed that (three) genus were found to degrade coprostanol, namely Achromobacter, Bacillus, and Branhamella. From 3 genus of coprostanol-degrading bacteria identified, 2 of them : Achromobacter, and Branhamella have not been reported so far.Key words : Coprostanol, Domestic Waste, Pollution Indicator, Isolation, Identification, Bacteria

Exploration, Isolation, and Identification of Carotenoid from Bacterial Symbiont of Sponge Callyspongia vaginalis Iqna Kamila Abfa; Ocky Karna Radjasa; A B Susanto; Handung Nuryadi; Ferry F. Karwur
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 2 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

During the past two decades research on marine bacteria has highlighted the tremendous potential of symbiotic-microorganisms as a source of bioactive secondary. One of the potential of the bacterial symbionts is producing a natural pigment, and these organisms can be used as a sustainable source of natural pigments. Carotenoid is one of the most important pigments that has important roles in physiological and molecular processes of microorganisms, as well as for human health. The objective of this study is to analyze carotenoid pigments from marine bacterial symbionts from sponge and to identify bacterial symbionts that produce carotenoid pigments. Pigment analysis was performed by a UV-VIS spectrophotometer and High Performance Liquid Chromatography (HPLC). Molecular bacterial identification was performed based on 16S rDNA sequence. The isolation of bacterial symbionts from C. vaginalison Zobell 2216E medium resulted in one bacterium, CB-SP5, positively synthesized carotenoids. By reverse phase HPLC analysis, the carotenoid pigments in the bacterial symbionts were identified as diadinoxanthin, fucoxanthin, neoxanthin, dinoxanthin, anddiadinochrome. CB-SP5 shared the highest level of 16S rDNA gene sequence similarity with Psychrobacter celer (99%). Keywords : carotenoid, sponge, bacterial symbiont, 16S rDNA.

Screening of Antibacterial MDR derived from Sponge Associated Fungus of Riung Water, Nusa Tenggara Timur Khoeruddin Wittriansyah; Agus Trianto; Sekar Widyaningsih; Ocky Karna Radjasa; Rudhi Pribadi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Marine sponge-associated fungi are the sources of bioactive compounds with various pharmacologicals potency. This study aimed to isolate the sponge-associated fungi as the producer of the MDR anti-bacterial compounds. The associated fungi were isolated from the sponges collected from Riung water, Nusa Tenggara Timur. Five of the best isolates were cultured on MEA to obtain the methanolic extract for further studies. The antagonistic test was conducted using overlay method towards the MDR Staphylococcus aureus and Escherichia coli. A total of 33 fungi were isolated from 19 sponge specimens. The antagonistic test showed that 19 isolates were active against both S. aureus and E. coli, and 13 of them were merely active against one of the bacteria. However, only five isolates have strong activity against one or both of the bacteria. The KN-15-3 had the strongest activity against S. aureus (18.75±0.777mm) and E. coli (15.10±0.141mm) at the concentration of 400 μg.disc-1 so it can be developed further as a source of drug candicate. Keywords: Fungi symbiont, Sponges, MDR Antibacterial, Staphylococcus aureus, Escherichia coli.

Causitive Agent Vibriosis dari Ikan Kerapu Bebek (Cromileptis altivelis) Bermulut Merah : 1. Patogenitas pada Ikan Kerapu Macan (Epinephelus fuscoguttatus) Sarjito Sarjito; Ocky Karna Radjasa; Sahala Hutabarat; Slamet B Prayitno
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 3 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The research aims were to find out the causative agent vibriosis of Cromileptis altivelis having clinical symptom red mouth and its pathogenecity to Epinephelus fuscoguttatus. Seven isolats Vibrio were isolated from wound and kidney of C. Altivelis. The result of Koch postulate indicated that five vibrios as a causative agent of vibriosis, consisted of three vibrios (isolat JT 07, JT 10, JT 20) and two vibrios (isolat JT 4, JT 29) caused mortality of 100% and 40% on E. fuscogutatus respectively. Three isolat vibrios ( JT 7, JT 10, JT 20 ) with higher pathogenicity were continued to futher investigation. Mean time to death of V. fuscus (JT 07), V. alginolyticus and V. anguillarum, to E. fuscogutatus on concentration of 108 CFU/mL were 83,33% (11,25 hours); 79,16%(15,63 hours); dan 50% (20,5 hours) respectively; whereas on concentration of 109 CFU/mL were 95,83% (10,8 hours); 87,5%(15,28 hours); dan 62,5% (19,6 hours) respectively. Lethal Concentration Median (LC50) of V. Fuscus, V. alginolyticus, V. anguillarum were 3,2X107 CFU/mL; 4,8 X 108 CFU/mL; dan 2,24X108 CFU/mL. All isolates on concentration of 106 dan 107 CFU/mL did not cause 50% tested fish mortality Key words : Causative agent, Vibriosis, E. Fuscogutatus, V. parahaemolyticus, Pathogenicity Penelitian ini bertujuan untuk mengkaji causative agent vibriosis pada ikan Kerapu Bebek (Cromileptis altivelis) dengan gejala klinis mulut merah serta patogenisitasnya terhadap ikan Kerapu Macan (Epinephelus fuscoguttatus). Tujuh isolat Vibrio berhasil diisolasi dari bagian luka dan ginjal Kerapu Bebek Mulut Merah. Hasil uji postulat koch memperlihatkan lima isolat dimana isolat JT 07, JT 10, JT 20 dapat mengakibatkan kematian 100%, sedangkan isolat JT 04 dan JT 29 menyebabkan kematian 40%. Pada tiga isolat (Vibrio JT 07, JT 10 dan JT 20) yang memperlihatkan patogenitas yang lebih tinggi dilakukan uji lanjutan. Hasil karakterisasi melalui uji morfologi dan biokimia diperoleh bahwa isolat JT 07 memiliki kemiripan 96,15 % dengan Vibrio fuscus; JT 10 memiliki kemiripan 100% dengan Vibrio anguillarum dan JT 20 memiliki kemiripan 100% dengan Vibrio alginolyticus. Patogenisitas ketiga isolat vibrio tersebut secara berurutan adalah V. fuscus, V. alginolyticus, V. anguillarum, dimana diperoleh bahwa persentase kematian dan rerata waktu kematian (Mean Time to Death, MTD) pada penyuntikan intraperitoneal V. fuscus (JT 07), V. alginolyticus (JT 20) dan V. anguillarum (JT 10) dengan konsentrasi 108 CFU/mL adalah berturut-turut 83,33% (11,25 jam); 79,16% (15,63 jam); dan 50% (20,5 jam); sedangkan untuk konsentrasi 109 CFU/mL secara berurutan adalah 95,83% (10,8 jam); 87,5% (15,28 jam); dan 62,5% (19,6 jam). Lethal Concentration Median (LC50) V. fuscus, V. alginolyticus, V. anguillarum secara berurutan adalah sebesar 3,2X107 CFU/mL; 4,8 X 108 CFU/mL; dan 2,24X108 CFU/mL. Sedang pada konsentrasi 106 dan 107 CFU/mL semua isolat tidak menimbulkan kematian pada ikan uji. Hasil ini menunjukkan bahwa tiga causative agent tersebut bersifat patogen pada ikan kerapu. Kata kunci: Causative agent, Vibriosis, E. fuscogutatus V. parahaemolyticus, Patogenisitas

Kajian Temporal Indeks Pencemaran Akibat Pengaruh Limbah Domestik dan Pola Penyebarannya di Perairan Pantai Kartini, Jepara Tonny Bachtiar; Uswatun Khasanah; Ocky Karna Radjasa; Edi Suhartono; Agus Hardiyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 4 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian ini dilakukan pada kondisi musim kemarau (Agustus 2003, monsun Timur), dan pada kondisi musim hujan (Maret 2004, kondisi monsun Barat), pada tiga kondisi lingkungan, yaitu: sungai dan muara Sungai Demaan, dan perairan pantai Kartini Jepara. Pengukuran beberapa parameter kualitas air dan pengambilan sampel air dilakukan pada 12 stasion. Dengan menggunakan metoda Indeks Pencemaran (pollution indexs) diketahui bahwa indeks pencemaran pada kondisi musim kemarau mempunyai nilai yang lebih tinggi dari pada kondisi musim hujan, baik di lingkungan sungai, muara, naupun perairan pantai. Kondisi lingkungan sungai pada kondisi musim kemarau mempunyai kriteria pencemaran cemar berat, dengan IP 11,48, pada kondisi musim hujan menjadi cemar ringan, dengan IP 4,49. Pada kondisi lingkungan muara saat musim kemarau, termasuk cemar sedang dengan IP 6,71, sedangkan pada kondisi musim hujan menjadi cemar ringan, dengan IP menjadi 3,11. Status mutu air di perairan pantai ke arah laut hingga stasiun kontrol termasuk dalam cemar ringan, baik pada kondisi musim kemarau maupun kondisi musim hujan. Secara umum pola penyebaran Indeks Pencemaran pada monsun Timur dan monsun Barat, mempunyai pola yang relatif tinggi di sekitar muara dan menurun dengan meningkatnya jarak dari muara, dan tersebar mengikuti dinamikaperairan ke Selatan, kemudian ke Baratdaya mengikuti bentuk pantai Teluk Kartini.Kata kunci: indeks pencemaran, monsun, muara, perairan pantai, sungaiThis research was conducted on the condition of dry season (August 2003, East monsoon), and the condition of wet season (March 2004, West monsoon), on three environmental conditions: river and river mouth ofDemaan River, and Kartini coastal waters, Jepara. Measurement of several water quality parameters and collecting of water samples was carried out at 12 stations. By using Pollution Index method, it could be recognized that pollution indexes (PI) in the river, river mouth, and coastal waters during dry season were higher than during wet season. In the river environment during dry season was heavy polluted with PI 11.48,and during wet season became light polluted with PI 4.49. River mouth environment during dry season was fairly polluted with PI 6.71, and during wet season became light polluted with PI 3.11. The status waterquality of coastal water to the sea direction until control station was in light polluted, both in dry and wet seasons. In general, the pattern distribution of PI in East and West monsoon were relatively higher around theriver mouth and decreased with increasing of distance from the river mouth, and distributed by dynamic of waters to the South, and then to the Southwest went along the coast of Kartini Bay.Key words: pollution index, monsoon, river mouth, coastal waters, river.

Analysis of Carotenoids and the Identification of Mangrove Sediment Bacteria of Segara Anakan, Cilacap Riyanti Riyanti; Meris Rahmawati; Nuning Vita Hidayati; Agung Dhamar Syakti; Ocky Karna Radjasa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 4 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The excessive consumption of artificial dyes can lead to negative effects on human body. Thus, the invention of natural dyes, such as carotenoids, is needed in order to reduce the negative impacts. Carotenoids are yellow, orange, and reddish yellow pigments produced by plants, animals, algae, and microorganisms. This study was aimed to analyze the carotenoid pigments of mangrove sediment bacteria in Segara Anakan, Cilacap, and to identify species of bacteria that can produce carotenoids. Carotenoids were analysed by using Thin Layer Chromatography (TLC) and UV-Vis spectrophotometer. Meanwhile molecular identification of bacteria was carried by 16S rDNA PCR and DNA sequence was analysed through a program called Basic Local Alignment Search Tool (BLAST). The kinship of bacteria was shown in Phylogenetic tree by using Bioedit and MEGA 5 software. Qualitative analysis by using TLC produced several pigments like: β-carotene, β-isorenieraten, lycopene, flavonoids, chlorophyll a, chlorophyll b and a feofitin with Rf value of 0.36-0.95. Quantitative analysis showed that bacteria KH (greenish yellow), KT (dark yellow) and KM (light yellow) produced carotenoids at 95.30 mg.g-1, 110.34 mg.g-1 and 25.349 mg.g-1. KH, KT and KM were suspected to be bacteria known as Streptomyces chartreusis, Bacillus megaterium and Streptomyces chromofuscus with similarity of 99%. Mangrove sediment bacteria had the potential of producing carotenoids as an alternative of eco-friendly natural dyes.

Karakterisasi Molekuler Bakteri yang Berasosiasi dengan Penyakit BBD (Black Band Disease) pada Karang Acropora sp di Perairan Karimunjawa Agus Sabdono; Ocky Karna Radjasa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 11, No 3 (2006): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Black Band Disease (BBD) merupakan penyakit yang bersifat virulen terutama menyerang jenis karang batu.Meskipun komunitas bakteri BBD didominasi oleh jenis cyanobakterium, namun penelitian tentang jumlahkomposisi bakteri yang menyusun komunitas belum pernah dilakukan. Komunitas bakteri yang berasosiasi dengan penyakit BBD (black band disease) pada karang cabang Acropora sp. dalam penelitian ini diujidengan menggunakan teknik kultur dependent. Teknik molekuler gen 16S rDNA (amplifikasi 16S DNA ribosom) digunakan untuk karakterisasi komunitas secara komprehensif. Berdasarkan analisis sekuen gen 16SrDNA, data menunjukkan bahwa isolate BBD1 memiliki kekerabatan terdekat dengan Myroides odoratimimus (99.0%), isolat BBD2 adalah Bacillus algicola (99.6%) dan isolat BBD3 adalah Marine Alcaligenaceae bacteriumico (96.0%). Hasil identifikasi bakteri yang berasosiasi dengan penyakit BBD pada karang cabang Acropora sp di Karimunjawa merupakan komunitas baru yang berbeda dengan hasil penelitian terdahulu. Hasil inimemungkinkan untuk dilakukan penelitian lanjutan tentang isolasi dan kultur bakteri tersebut untuk bisa menerangkan etiologi penyakit.Kata kunci: Black Band Disease (BBD), Acropora sp., 16S rDNA.Black band disease (BBD) is a virulent disease primarily affecting scleractinian corals. Eventhough the BBD bacterial mat is dominated by a cyanobacterium, the quantitative composition of the BBD bacterial matcommunity has not described previously. The bacterial community associated with black band disease (BBD) of the branching corals Acropora sp. in this study was examined using culture-dependent techniques. Acomplementary molecular techniques of 16S rDNA genes [amplified 16S ribosomal DNA) was used to give a comprehensive characterization of the community. On the basis of the results of sequen analysis, our datashow that BBD1 isolate was closely related with Myroides odoratimimus (99.0%), BBD2 isolate was Bacillus algicola (99.6%) and BBD3 isolate was Marine Alcaligenaceae bacterium (96.0%). Of the three bacteria identified,these were not previously found in other studies. This result will allow the dominant BBD bacteria to be targeted for isolation and culturing experiments designed at interpreting the disease etiology.Key words: Black Band Disease, Acropora sp., 16S rDNA.

Kandungan Koprostanol dan Bakteri Coliform Pada Lingkungan Perairan Sungai, Muara, dan Pantai di Banjir Kanal Timur, Semarang Pada Monsun Timur Tri Yuni Atmojo; Tony Bachtiar; Ocky Karna Radjasa; Agus Sabdono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 1 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Limbah domestik merupakan salah satu sumber utama pencemaran di perairan pantai pada negara yang sedang berkembang yang masih kurang mendapatkan perhatian serius bila dibandingkan dengan pencemaran oleh industri. Namun dengan terus meningkatnya aktivitas manusia di wilayah pesisir dan kesadaran akan pentingnya lingkungan bersih bagi kesehatan, estetika dan alasan ekologis lainnya, deteksi tentang kontaminasi limbah menjadi penting untuk diketahui secara lebih baik. Selama ini indikator kontaminasi limbah domestik ditentukan berdasarkan jumlah mikroorganisme intestinal khususnya kelompok bakteri coliform. Koprostanol diusulkan sebagai alternatif indikator limbah domestik, sehingga diperlukan kajian eksistensi koprostanol untuk persyaratan kelayakannya sebagai indikator, serta bakteri coliform sebagai pembanding. Penelitian dilakukan pada bulan Juli-Agustus 2003 pada lingkungan sungai, muara dan pantai di sungai Banjir Kanal Timur di Semarang. Analisa coliform dari sampel air dan sedimen dilakukan di laboratorium Mikrobiogenetika, FMIPA UNDIP Semarang dan analisa konsentrasi koprostanol dilakukan di laboratorium Kimia dan FisikaPusat (LAKFIP) UGM Yogyakarta. Hasil menunjukkan bahwa koprostanol dapat terdeteksi pada sedimen dan tidak terdeteksi pada kolom air. Eksistensi koprostanol didapatkan nilai tertinggi pada lingkungan perairansungai (14,9 μg/g) dibandingkan muara (1,04 μg/g), dan pantai (5,25 μg/g). Bakteri total coliform terdeteksi pada kolom air maupun sedimen pada lingkungan perairan sungai (2,80 x 104 sel/100 ml), muara (0,4 x104 sel/100 ml), dan pantai (0 - 0,4 x 104) sel/100 ml, sementara fecal coliform terdeteksi di lingkungan perairan sungai (2 x 104 - 2,8 x 104 ) sel/100 ml, dan muara (0,4 x 104 sel/100 ml), namun tidak terdeteksipada lingkungan perairan pantai.Kata kunci : Koprostanol, limbah domestik, indikator pencemaran, coliformDomestic waste is one of the major sources of the pollution in coastal waters of most developing, countries, which has got less attention than industrial pollution. However, along with the increase of human activitiesin coastal areas coupled with the importance of clean environment for the health, esthetics, and ecological reasons, the detection of waste contamination has become important to be recognized. So far, the indicator of domestic waste contamination has been intestinal microorganism, especially coliform bacteria. Coprostanol is a proposed alternative indicator in the detected domestic waste those, it is definitely important to study the existence of coprostanol and coliform bacteria in order to fine its application. The research carried out from July to August 2003 at the environmental waters of river, estuarine, and coastal of Ciliwung, Jakarta; Banjir Kanal Timur, Semarang, analysis of coliform form water and sediment samples was conducted at micro biogenetic laboratory Faculty of Mathematic and Natural sciences, Diponegoro University, meanwhile analysis of coprostanil concentration was performed at central laboratory of chemistry and physic UGM. The results showed that coprostanol was detected in sediment but not in the water phase at all locations. The existence of coprostanol was detected either at environmental waters of river (14,9 μg/g) or estuarine (1,04 μg/g), and coastal (5,25 μg/g). Total coliform bacteria were detected both in water column and sediment of river (2,80 x 104 ) sel/100 ml, at estuarine (0,4 x 104) sel/100 ml and coastal (0 - 0,4 x104) sel/100 ml, meanwhile fecal coliform bacteria were detected in environmental water of river (2 x 104 - 2,8 x 104 ) sel/ 100 ml, and at estuarine (0 - 4 x 104) sel/100 ml, but not detected at coastal area. The use of coprostanol to considered for the indicator alternative because the existence can influence by various condition at water of river and used of coliform bacteria as domestic waste contamination indicator require to be considered because owning various weakness.Key words : Coprostanol, coliform, domestic waste, pollution indicator

Co-Authors A. Sabdono ADI SANTOSO Agung Dhamar Syakti Agus Hardiyanto Agus Sabdono Agus Sabdono Agus Trianto Ali Ridlo Ambariyanto , Aninditia Sabdaningsih Antonius Budi Susanto Awang Surya Wiguna Awang Surya Wiguna, Awang Surya Bahry, Muhammad Syaifudien Bambang Yulianto Bayu Munandar Bintang Marhaeni Boedi Hendrarto Busaeri, Nundang Delianis Pringgenies Delianis Pringgenies Desy Wulan Triningsih Dietrich G Bengen Dietriech G. Bengen Dietriech Geoffrey Bengen Duhita Sinidhikaraning Kencana Dwi Haryo Ismunarti Elena Zocchi Endang Sri Lestari Endang Supriyantini Endang Supriyantini Fauziah Shahul Hamid Fera Nur Idawati Sahara Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu Ferry Fredy Karwur Gede Iwan Setiabudi Gede Iwan Setiabudi Haeruddin Haeruddin Handung Nuryadi HANS- PETER GROSSART Hariyadi, Putut Hefni Effendi Hendi Perdian Yunianto Hendi Perdian Yunianto Herawati Sudoyo Hidetoshi Urakawa Hidetoshi Urakawal Ika Wulan Santi Ika Wulan Santi Iqna Kamila Abfa Irfan Dwidya Prijambada Isai Yusidharta Ita Riniatsih Ita Widowati JAKA WIDADA JOEDORO SOEDARSONO Johannes Hutabarat Kouichi Ohwada Kouichi Ohwadai Kumiko Kita-Tsukamoto Kumiko Kita-Tsukamotoz Leenawaty Limantara Lia Kusmita Lia Kusmita Mada Triandala Sibero Mada Triandala Sibero McCauley, Erin Meinhard Simon Meris Rahmawati Meutia Samira Ismet Miftahuddin M Khoeri Miftahuddin Majid Khoeri Misbakul Munir Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Mujizat Kawaroe Mukti K. Diana Putri Mutiara, Frista Nestin E.W. Ningrum Ni Komang Tri Utami Norma Afiati Nuning Vita Hidayati Nurhayati Nurhayati Nurkhasanah, Wulan Nurmanita Rismaningsih Nurul Hiron, Nurul Nurul Ria Arlita Paulus Damar Bayu Murti Pinandoyo Pinandoyo Prastyo Abi Widyananto Putut Har Riyadi R. Riyanti Rachmat Afriyanto Richardus F Kaswadji Rismaningsih, Nurmanita Riyanti Riyanti . Riyanti Riyanti Riyanti Riyanti Riyanti, Rory Anthony Hutagalung Rr Citra Permata Rr Citra Permata Rr. Citra Permata Rr. Citra Permata Rudhi Pribadi Rudiger Stöhr S arjito S. Sulistiyani Saefuddin Aziz Sahala Hutabarat Sakti Imam Muchlisin Sakti Imam Muchlissin Sakti Imam Muchlissin Sarjito - Sekar Widyaningsih Septhy Kusuma Radjasa Seto Windarto Slamet B Prayitno slamet budi prayitno Slamet Budi Prayitno Sri Achadi Nugraheni Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Suharsono . Suhartono, Suhartono Supriharyono ,, Supriharyono Suyono ,, Suyono Suyono Suyono Tedi Septiadi Tonny Bachtiar TONNY BACHTIAR Tonny Bachtiar Tony Bachtiar Tony Bachtiar TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Uswatun Khasanah Vita Meylani Vivi Endar Herawati VIVI ENDAR HERAWATI Windarto, Seto Wiratno Wiratno Wismayanti, Gita Wittriansyah, Khoeruddin Wiwik Astuti Wiwik Astuti Wulan Nurkhasanah YS Darmanto Yustin Widyastuti

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