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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Rekayasa Proses JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia OSEATEK DIPOIPTEKS: Jurnal Ilmiah Mahasiswa Undip Jurnal Matematika & Sains Prosiding Indonesian Journal of Pharmaceutical Science and Technology Proceeding Seminar LPPM UMP Tahun 2014 Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Marine Research in Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Rekayasa Proses Indonesian Journal of Energy Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding University Research Colloquium Prosiding Konferensi Nasional Penelitian Matematika dan Pembelajarannya

Ocky Karna Radjasa

Badan Riset Dan Inovasi Nasional

Author-ID : 295934

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Other

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Pigments Characterization and Molecular Identification of Bacterial Symbionts of Brown Algae Padinasp. Collected from Karimunjawa Island Damar Bayu Murti; A B Susanto; Ocky Karna Radjasa; Ferdy Semuel Rondonuwu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 2 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The search for carotenoids in nature has been extensively studied because of their applications in foods. One treasure of the biopigment source is symbiotic-microorganisms with marine biota. The advantages of symbiont bacteria are easy to culture and sensitize pigments. The use of symbiont bacteria helps to conserve fish, coral reefs, seagrass, and seaweed. Therefore, the bacteria keeps their existence in their ecosystems. In this study, bacterial symbionts were successfully isolated from brown algae Padina sp. The bacterial symbionts had yellow pigment associated with carotenoids. The pigments were characterized using High Performance Liquid Chromatography (HPLC) with a Photo Diode Array (PDA) detector. The carotenoid pigments in the bacterial symbionts were identified as dinoxanthin, lutein and neoxanthin. Molecular identification by using a 16S rRNA gene sequence method, reveals that the bacterial symbionts were closely related to Bacillus marisflavi with a homology of 99%. Keywords :carotenoid pigments, brown algae, Padina, bacterial symbionts, 16S rRNA

Biodegradation of Polyethylene Microplastic using Culturable Coral-Associated Bacteria Isolated from Corals of Karimunjawa National Park Prastyo Abi Widyananto; Sakti Imam Muchlissin; Agus Sabdono; Bambang Yulianto; Fauziah Shahul Hamid; Ocky Karna Radjasa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 4 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.4.237-246

Polyethylene is a plastic material that was globally produced and is well known as a non-degradable pollutant product. Plastic pollution, primarily microplastics, have been distributed to coral reef ecosystems, where these areas are ecosystems with high productivity. Karimunjawa National Park in Indonesia is one of the protected areas for coral reef ecosystem habitat in Central Java, threatened by microplastic contamination. Recent studies have shown that coral-associated bacteria have an adequate ability to degrade marine pollutant materials. No one has reported that the use of indigenous coral-associated bacteria has the potential for microplastic biodegradation, especially low-density polyethylene microplastic materials. Hence, the objective of this study was to find the potential of microplastic biodegradation agents derived from coral-associated bacteria in Karimunjawa National Park area. Various coral life-forms were isolated in July 2020 from conservation areas and areas with anthropogenic influences. Bacterial isolates were screened using tributyrin and polycaprolactone as substrates to reveal potential microplastic degradation enzymes. The total isolation results obtained 92 bacterial isolates, and then from the result of enzyme screening, there were 7 active bacteria and only 1 bacteria that potential to degrade polyethylene. LBC 1 showed that strain could degrade by 2.25±0.0684 % low-density polyethylene microplastic pellet by incubating bacterial growth until the stationary phase. Identification of LBC 1 strain was carried out by extracting DNA and bacterial 16S rRNA sequences. Bacterial gene identification refers to Bacillus paramycoides with a similarity level in the National Center Biotechnology Information database of 99.44%. These results prove that hard coral association bacteria can degrade low-density polyethylene microplastics.

Peningkatan Kualitas Air Pantai Menjadi Air Bersih dengan Penerapan Teknologi Plasma Non-Thermal dan Multi-Step Filter Rr Citra Permata; Ocky Karna Radjasa
DIPOIPTEKS: Jurnal Ilmiah Mahasiswa Undip DIPOIPTEKS Vol. 1, No. 1 (2013)
Publisher : DIPOIPTEKS: Jurnal Ilmiah Mahasiswa Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.68 KB)

Pigmen karotenoid yang diproduksi oleh bakteri simbion lamun Thalassia hemprichii berhasil diisolasi berasal dari perairan Teluk Awur, Jepara. Pigmen karotenoid merupakan pigmen berwarna merah, orange dan kuning yang disinyalir memiliki fungsi sebagai antioksidan. Isolat bakteri pigmen karotenoid berjumlah 5 isolat yaitu TH1, TH4, TH6, TH18 dan TH20 namun hanya 1 isolat yang mempunyai warna terkuat yaitu isolat dengan kode TH1. Isolate TH1 kemudian diuji DPPH untuk mengetahui kadar antioksidannya. Dari hasil uji DPPH didapatkan kadar antioksidandengan persen penghambatan 31,11%.Kata kunci: Pigmen karotenoid, Bakteri Simbion Thalassia hemprichii, Uji DPPH, Antioksidan

Pemanfaatan Jamur Simbion Sponge dalam Bioisomerasi Asam Lemak pada Dedak untuk Menghasilkan Asam Lemak Cis Agus Trianto; Sri Sedjati; Ocky Karna Radjasa; Rachmat Afriyanto; Sakti Imam Muchlisin; Septhy Kusuma Radjasa; Muhammad Syaifudien Bahry
Jurnal Kelautan Tropis Vol 21, No 2 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Utilization of Sponges Symbiotic Fungus in the Bioisomeration of Fatty Acids in Brans to Produce Cis Fatty Acid Trans fatty acids are known to have a negative impact on human health such as triggering the coronary heart and blood vessel related diseases. However, many food and animal feed contain trans fatty acids. Bran, which is widely used as animal feed has high fatty acid content and the majority are trans fatty acids. This study aims to determine the potential of Trichoderma harzanum, a sponge symbiont fungus, in the process of fatty acid bioconversion in bran. Bran was fermented in saline and non-salin condition for 15 days in the room temperature. Both fermented and not bran was extracted with methanol, and the extracts were concentrated with rotary evaporator. Fatty acid extracts were analyzed on the GC with hydrolysis and methylation as pre-treatments. The test results showed the fatty acid composition changed, and the was formation of new fatty acids. The bioisomeration process occurs in the Trans-9-Elaidic acid Methyl esther into Cis-9-Oleic Methyl ester. Cis-9-oleic acid is known as oleic acid which is widely found in olive and sunflowers oil. The fungus has potential as biocatalys for production of cis fatty acid. Asam lemak trans dikenal mempunyai dampak negatif terhadap kesehatan manusia seperti memicu terjadinya penyakit jantung koroner dan penyakit terkait pembuluh darah. Namun asam lemak trans banyak terkandung dalam berbagai jenis bahan makanan maupun pakan ternak diantaranya adalah dedak. Dedak banyak digunakan sebagai pakan ternak mempunyai kandungan asam lemak yang tinggi dan mayoritas adalah asam lemak trans. Penelitian ini bertujuan untuk mengetahui potensi jamur simbion spons Trichoderma harzanum dalam proses biokonversi asam lemak pada dedak. Fermentasi dedak dilakukan dalam kondisi salin dan unsalin selama 15 hari pada suhu ruang. Dedak yang difermentasi maupun tidak kemudian diekstrak dengan metanol kemudian dipekatkan dengan rotari eveporator. Ekstrak asam lemak degan GC dengan pre-treatment meliputi hidrolisis dan metilasi. Hasil uji menunjukan adanya perubahan komposisi dan terbentuknya asam lemak baru. Proses bioisomerasi terjadi pada Trans-9-Elaidic acid Methyl estermenjadi Cis-9-Oleic Methyl ester. Cis-9-oleic acid yang dikenal dengan asam oleat yang banyak terdapat dalam minyak zaitun dan bunga matahari. Jamur T. Harzianum mempunyai potensi sebagai biokatalis untuk memproduksi asam lemak cis.

Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli Mada Triandala Sibero; Aninditia Sabdaningsih; Ocky Karna Radjasa; Agus Sabdono; Agus Trianto; Subagiyo Subagiyo
Jurnal Kelautan Tropis Vol 22, No 1 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

The Trichoderma asperellum MT02 has been reported to has antibacterial activity against the Extended Spectrum β-Lactamase (ESBL) E. coli based on the screening results through an agar plug method. This study aimed to evaluate the antibacterial activity of T. asperellum MT02 and characterize the composition of the bioactive compounds group possessed in its crude extract. The isolate was cultured in the Malt Extract Broth (MEB) media (static, 27 oC, 15 days). The intracellular metabolites from mycelium were extracted using methanol while extracellular metabolites from broth media were extracted using ethyl acetate. The antibacterial activity of crude extracts was tested using the paper disc diffusion method while bioactive compounds were characterized using the phytochemical method. The results showed that the antibacterial activity of the broth media extract performed a greater activity than the crude extract from the mycelium. The crude extract from mycelia only contained flavonoid and phenol hydroquinone compounds while the crude extract from broth media contains alkaloids, flavonoids, phenols hydroquinone and saponins. Kapang Trichoderma asperellum MT02 telah dilaporkan memiliki potensi sebagai penghasil senyawa antibakteri melawan Extended Spectrum β-Lactamase (ESBL) E. coli berdasarkan hasil penapisan melalui metode agar plug. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kasar kapang T. asperellum MT02 serta komposisi golongan senyawa bioaktif yang dimiliki. Kapang dikultur pada media Malt Extract Broth (MEB) (statis, 27 oC, 15 hari) di mana metabolit intraseluler dari miselium diekstrak menggunakan metanol sedangkan metabolit ekstraseluler dari media kaldu diekstrak menggunakan etil asetat. Aktivitas antibakteri ekstrak kasar diuji menggunakan metode difusi kertas cakram sedangkan senyawa bioaktif dikarakterisasi menggunakan metode fitokimia. Hasil penelitian menunjukkan bahwa aktivitas antibakteri asal ekstrak media kaldu lebih baik dibandingkan ekstrak kasar asal miselium kapang. Ekstrak kasar kapang asal miselia hanya mengandung senyawa golongan flavonoid dan fenol hidrokuinon sedangkan ekstrak kasar asal media kaldu mengandung alkaloid, flavonoid, fenol hidroquinon dan saponin.

Kelimpahan Mikroplastik Pada Sedimen Ekosistem Terumbu di Taman Nasional Laut Karimunjawa Sakti Imam Muchlissin; Prastyo Abi Widyananto; Agus Sabdono; Ocky Karna Radjasa
Jurnal Kelautan Tropis Vol 24, No 1 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i1.9865

Eleven billion microplastic particles are entangled in coral reef ecosystems in the Asia - Pacific Region. The presence of microplastics in coral reef ecosystems in Indonesia, especially in the Karimunjawa Marine National Park, was found as many as 22.7 and 12.8 particles / kilogram samples in two locations. The presence of microplastics in coral reef ecosystems allows threats to the health of coral reefs. Therefore, the importance of this study is to complement the data on the distribution of microplastics in the Karimunjawa Marine National Park as an initial step for conservation and mitigation of the impact of plastic/ microplastic pollution. Sampling using purposive sampling method. Sediment collection using SCUBA set and sediment grab at a depth of 3 - 5 meters. ± 1000 g of sediment was taken and stored in double zip lock plastic. The samples were then analyzed by microplastics in the Tropical Marine Biotechnology Laboratory, FPIK UNDIP. The results showed that the greatest abundance of microplastics was in the Karang Tengah area with the number of microplastics 96 particles / kilogram, and the farthest in the Ujung Gelam and Lego was 11 particles / kilogram. Research shows that the abundance of microplastics in areas with human activities such as tourism, ports, and boat routes has a high abundance compared to conservation areas or areas with little human activity. It is hoped that the available data from this type of research will be able to produce decisions on conservation measures in Karimunjawa Marine National Park. Sebelas miliar partikel mikroplastik terjerat pada ekosistem terumbu karang di Kawasan Asia – Pasifik. Keberadaan mikroplastik pada ekosistem terumbu karang di Indonesia, khususnya di Taman Nasional Laut Karimunjawa, ditemukan sebanyak 22,7 dan 12,8 partikel/kilogram sampel di dua lokasi. Keberadaan mikroplastik di ekosistem terumbu karang memungkinkan adanya ancaman terhadap kesehatan terumbu karang. Oleh karena itu pentingnya penelitian ini untuk melengkapi data sebaran mikroplastik di Kawasan Taman Nasional Laut Karimunjawa sebagai langkah awal konservasi dan mitigasi dari dampak polusi plastik/mikroplastik. Pengambilan sampel menggunakan metode purposive sampling. Pengambilan sedimen menggunakan SCUBA set dan sediment grab pada kedalaman 3 – 5 meter. Sedimen diambil ± 1000 gr dan disimpan dalam plastik double zip lock. Sampel kemudian dianalisis mikroplastik di laboratorium Tropical Marine Biotechnology, FPIK UNDIP. Hasil menunjukkan bahwa kelimpahan mikroplastik terbesar pada daerah karang tengah dengan jumlah mikroplastik 96 partikel/kilogram, dan terkecil pada perairan ujung gelam dan lego yaitu sebanyak 11 partikel/kilogram. Penelitian menunjukkan bahwa kelimpahan mikroplastik didaerah dengan aktivitas manusia seperti pariwisata, pelabuhan, dan jalur kapal memiliki kelimpahan tinggi dibanding dengan daerah konservasi atau daerah dengan aktivitas manusia yang kecil. diharapkan dengan data yang tersedia dari penelitian sejenis ini, mampu menghasilkan keputusan terhadap langkah konservasi di Taman Nasional Laut Karimunjawa.

Skrining Aktivitas Antibakteri Dan Identifikasi Sponge Dari Teluk Kupang Agus Trianto; Ni Komang Tri Utami; Ocky Karna Radjasa; Isai Yusidharta; Wiratno Wiratno
Jurnal Kelautan Tropis Vol 19, No 2 (2016): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Recently, many research proved that bacteria Escherichia coli and Staphylococus aureus have resistant to antibiotics, called Multi Drug Resistance (MDR). The bacteria cause various diseases both in humans and animals. Exploration and development of new antibiotics is needed, one which is derived from a sea sponge. This research aims to determine the potential of antibacterial sponge extracts from the waters of Kupang, East Nusa Tenggara against E. coli and S. aureus MDR, as well as being able to identify the sponge. Maceration method was employed on extraction of the sponges, then the extract were tested against E. coli and S. aureus. The extracts content of sponge varied from 0.4% to 5,19%. Anti bacterial test showed that the sponge K14-52 has the highest inhibition zone of 10,43 ± 0,26 mm and 9,38 ± 0,57 mm and against the E. coli and S. Aureus, respectively, at a concentration of 500 ug/disk. Based on macroscopic and microscopic anayses, the K14-52 sponge is identified as Rhabdastrella globostellata. a Demospongia sponge. The sponge has special characteristic i.e. globular shape with large oscula on the top of the sponge and ostia along the surface. The sponge also characterized with makrosclera monoaxon spikule (hastate oxea, centrotylote oxea, oxea fusiform gyrus) and mikrosclera tertaxon oxyaster spikule. Saat ini banyak ditemukan bakteri Escherichia coli dan Staphylococus aureus yang resisten terhadap antibiotik atau disebut Multi Drugs Resistance (MDR). Bakteri tersebut dapat menimbulkan berbagai penyakit baik pada manusia maupun hewan. Eksplorasi dan pengembangan sumber antibiotik baru sangat diperlukan, salah satunya yang berasal dari sponge laut. Penelitian ini bertujuan untuk mengetahui potensi antibakteri ekstrak sponge dari Perairan Kupang, Nusa Tenggara Timur terhadap bakteri E. coli dan S. aureus MDR, serta mampu mengetahui jenis sponge. Ekstraksi dilakukan dengan metoda maserasi kemudian ekstrak diuji terhadap bakteri patogen. Uji antibakteri menunjukkan bahwa sponge K14-52 memiliki potensi antibakteri paling tinggi dengan zona hambat sebesar 10,43±0,26 mm terhadap bakteri E. coli dan 9,38±0,57 mm terhadap bakteri S. aureus pada konsentrasi 500 µg/disk. Sponge K14-52 diidentifikasi sebagai Rhabdastrella globostellata berdasarkan analisis secara makroskopis dan mikroskopis. Ciri khas sponge tersebut adalah mempunyai bentuk bulat dengan ostia yang tersebar pada permukaan dan satu Oskula besar pada bagian atas sponge. Secara mikroskopis sponge tersebut memiliki makrosklera monoaxon spikula (hastate oxea, centrotylote oxea, fusiform oxea) dan mikrosklera tertaxon spikula Oxyaster.

Ecological Role of A Softcoral-Associated Bacterium Arthrobacter sp. on Marine Biofilm-Forming Bacteria OCKY KARNA RADJASA; AGUS SABDONO
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

A marine bacterium species associated with softcoral Sinularia sp. collected from vicinity of Peucang island, Ujung Kulon, West Java, was successfully screened for estimating its ecological role through inhibiting marine biofilm-forming bacteria isolated from the surrounding colonies of Sinularia sp. and was identified as closely related to Arthrobacter nicotianae based on its 16S rDNA structure. The bacterium was found to inhibit the growth of four biofilm-forming isolates (Vibrio harveyi, V. fortis, Staphylococcus sciuri, and Tenacibaculum marilutum) indicating the significance of secondary metabolite production which may provide important defensive functions against fouling microorganisms. The isolate was capable of amplifying gene fragments of non-ribosomal peptide synthetases. A 416 bp long DNA fragment was obtained and the deduced amino acid sequence showed conserved signature regions for the peptide synthetases and revealed a high similarity to that of Actinoplanes teichomyceticus (62.5% identity).

Pengaruh Musim dan Pasang Surut Terhadap Konsentrasi Mikroplastik di Perairan Delta Sungai Wulan, Kabupaten Demak Sri Yulina Wulandari; Ocky Karna Radjasa; Bambang Yulianto; Bayu Munandar
Buletin Oseanografi Marina Vol 11, No 2 (2022): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v11i2.46329

Sungai merupakan jalur penting masuknya mikroplastik ke perairan laut. Mikroplastik baik primer atau sekunder merupakan polutan partikel plastik yang berukuran kurang dari 5 mm (ukuran partikel mirip dengan partikel suspensi atau plankton). Termasuk sampah plastik, mikroplastik berpotensi menyebabkan terganggunya rantai makanan pada biota laut. Penelitian ini bertujuan untuk mengetahui konsentrasi mikroplastik di kolom air pada musim hujan dan kemarau saat kondisi pasang dan surut di perairan Delta Wulan, Kecamatan Wedung Kabupaten Demak. Metode yang digunakan adalah deskriptif kuantitatif. Total sebanyak 32 sampel air laut diambil dengan menggunakan plankton net dari 8 stasiun pengamatan yang ditentukan secara purposive. Pengambilan sampel dilakukan dengan mempertimbangkan kondisi musim dan pasang surut. Analisa kandungan mikroplastik dalam sampel air laut dilakukan dengan menggunakan metode yang direkomendasikan oleh NOAA. Prosedur analisa meliputi penyaringan sampel air, penambahan reagen (Fe (II) 0,05M, H2O2 30%, NaCl), pemanasan, pemisahan berdasarkan densitas dan analisa gravimeri, Hasil penelitian menunjukkan bahwa nilai konsentrasi mikroplastik lebih tinggi pada musim kemarau dibanding musim hujan, dengan nilai rerata berturut-turut sebesar 2,608 mg/L dan 2,247 mg/L. Dari analisa regresi ANOVA menunjukkan ada perbedaan konsentrasi berdasarkan musim (p-value sebesar 0,007), namun tidak ada perbedaan berdasarkan kondisi pasang dan surut (p-value sebesar 0,840). Rivers are an important pathway for the entry of microplastics into marine waters. Microplastics, both primary and secondary, are pollutants of plastic particles that are less than 5 mm in size (particle size is similar to sediment suspense or plankton). Including plastic waste, microplastics have the potential to disrupt the food chain in marine biota. This study aims to determine the concentration of microplastics in the water column during the rainy and dry seasons during high and low tide conditions in the waters of the Wulan Delta, Wedung District, Demak Regency. The method used is descriptive quantitative. A total of 32 seawater samples were taken using a plankton net from 8 observation stations which were determined purposively. Sampling was carried out by considering seasonal and tidal conditions. Analysis of microplastic content in seawater samples was carried out using the procedure recommended by NOAA. The analytical procedure includes filtering of water samples, addition of reagent (Fe (II) 0.05M, 30% H2O2, NaCl), heating, density separation and gravimetric analysis. The results showed that the concentration of microplastics was higher in the dry season than in the rainy season, with an average value of 2,608 mg/L and 2,247 mg/L, respectively. From the ANOVA regression analysis showed that there was a difference in concentration based on seasons (p-value of 0.007), but there was no difference based on tidal and tidal conditions (p-value of 0.840).

EKSISTENSI KOPROSTANOL DAN BAKTERI COLIFORM PADA LINGKUNGAN PERAIRAN SUNGAI, MUARA, DAN PANTAI DI JEPARA PADA MONSUN TIMUR Tri Yuni Atmojo; Tony Bachtiar; Ocky Karna Radjasa; Agus Sabdono
Jurnal Ilmu Lingkungan Vol 9, No 1 (2011): April 2011
Publisher : School of Postgraduate Studies, Diponegoro Univer

Limbah domestik merupakan salah satu sumber utama pencemaran di perairan pantai pada negara yang sedang berkembang, namun kurang mendapatkan perhatian serius dibandingkan dengan pencemaran oleh industri. Dengan terus meningkatnya aktivitas manusia di wilayah pesisir dan kesadaran akan pentingnya lingkungan bersih bagi kesehatan, estetika dan alasan ekologis lainnya, deteksi tentang kontaminasi limbah menjadi penting untuk diketahui secara lebih baik. Selama ini indikator kontaminasi limbah domestik ditentukan berdasarkan jumlah mikroorganisme intestinal khususnya kelompok bakteri coliform. Koprostanol diusulkan sebagai alternatif indikator limbah domestik, sehingga diperlukan kajian eksistensi koprostanol untuk persyaratan kelayakannya sebagai indikator, serta bakteri coliform sebagai pembanding.Penelitian dilakukan lingkungan sungai, muara dan pantai di sungai Ciliwung Jakarta. Hasil menunjukkan bahwa koprostanol dapat terdeteksi pada sedimen dan tidak terdeteksi pada kolom air. Eksistensi koprostanol didapatkan nilai tertinggi pada lingkungan perairan sungai (5,81μg/g) dibandingkan muara (5,63μg/g), dan pantai (2,93μg/g). Bakteri total coliform terdeteksi pada kolom air maupun sedimen pada lingkungan perairan sungai (2,8 x 104 - 4,3 x 104) sel/100 ml, muara (0 - 4 x 103) sel/100 ml, dan tidak terdeteksi pada lingkungan perairan pantai, sementara fecal coliform terdeteksi di lingkungan perairan sungai (2 x 104 - 4,3 x 104 ) sel/100 ml, dan muara (0 - 4 x 104 sel/100 ml), namun tidak terdeteksi pada lingkungan perairan pantai.

Co-Authors A. Sabdono ADI SANTOSO Agung Dhamar Syakti Agus Hardiyanto Agus Sabdono Agus Sabdono Agus Trianto Ali Ridlo Ambariyanto , Aninditia Sabdaningsih Antonius Budi Susanto Awang Surya Wiguna Awang Surya Wiguna, Awang Surya Bahry, Muhammad Syaifudien Bambang Yulianto Bayu Munandar Bintang Marhaeni Boedi Hendrarto Busaeri, Nundang Delianis Pringgenies Delianis Pringgenies Desy Wulan Triningsih Dietrich G Bengen Dietriech G. Bengen Dietriech Geoffrey Bengen Duhita Sinidhikaraning Kencana Dwi Haryo Ismunarti Elena Zocchi Endang Sri Lestari Endang Supriyantini Endang Supriyantini Fauziah Shahul Hamid Fera Nur Idawati Sahara Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu Ferdy Semuel Rondonuwu Ferry Fredy Karwur Gede Iwan Setiabudi Gede Iwan Setiabudi Haeruddin Haeruddin Handung Nuryadi HANS- PETER GROSSART Hariyadi, Putut Hefni Effendi Hendi Perdian Yunianto Hendi Perdian Yunianto Herawati Sudoyo Hidetoshi Urakawa Hidetoshi Urakawal Ika Wulan Santi Ika Wulan Santi Iqna Kamila Abfa Irfan Dwidya Prijambada Isai Yusidharta Ita Riniatsih Ita Widowati JAKA WIDADA JOEDORO SOEDARSONO Johannes Hutabarat Kouichi Ohwada Kouichi Ohwadai Kumiko Kita-Tsukamoto Kumiko Kita-Tsukamotoz Leenawaty Limantara Lia Kusmita Lia Kusmita Mada Triandala Sibero Mada Triandala Sibero McCauley, Erin Meinhard Simon Meris Rahmawati Meutia Samira Ismet Miftahuddin M Khoeri Miftahuddin Majid Khoeri Misbakul Munir Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Mujizat Kawaroe Mukti K. Diana Putri Mutiara, Frista Nestin E.W. Ningrum Ni Komang Tri Utami Norma Afiati Nuning Vita Hidayati Nurhayati Nurhayati Nurkhasanah, Wulan Nurmanita Rismaningsih Nurul Hiron, Nurul Nurul Ria Arlita Paulus Damar Bayu Murti Pinandoyo Pinandoyo Prastyo Abi Widyananto Putut Har Riyadi R. Riyanti Rachmat Afriyanto Richardus F Kaswadji Rismaningsih, Nurmanita Riyanti Riyanti . Riyanti Riyanti Riyanti Riyanti Riyanti, Rory Anthony Hutagalung Rr Citra Permata Rr Citra Permata Rr. Citra Permata Rr. Citra Permata Rudhi Pribadi Rudiger Stöhr S arjito S. Sulistiyani Saefuddin Aziz Sahala Hutabarat Sakti Imam Muchlisin Sakti Imam Muchlissin Sakti Imam Muchlissin Sarjito - Sekar Widyaningsih Septhy Kusuma Radjasa Seto Windarto Slamet B Prayitno slamet budi prayitno Slamet Budi Prayitno Sri Achadi Nugraheni Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Suharsono . Suhartono, Suhartono Supriharyono ,, Supriharyono Suyono ,, Suyono Suyono Suyono Tedi Septiadi Tonny Bachtiar Tonny Bachtiar TONNY BACHTIAR Tony Bachtiar Tony Bachtiar TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Uswatun Khasanah Vita Meylani Vivi Endar Herawati VIVI ENDAR HERAWATI Windarto, Seto Wiratno Wiratno Wismayanti, Gita Wittriansyah, Khoeruddin Wiwik Astuti Wiwik Astuti Wulan Nurkhasanah YS Darmanto Yustin Widyastuti

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