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All Journal HAYATI Journal of Biosciences Agrotrop : Journal on Agriculture Science METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS BIOMA : Jurnal Biologi Makassar Journal of Health Sciences and Medicine Indonesian Biodiversity Journal Indonesian Biodiversity Journal
Retno Kawuri
Program Studi Biologi Fakultas Matematika Ilmu Pengetahuan Alam, Universitas Udayana
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Journal : Jurnal Biologi Udayana

 1 
Keberadaan bakteri patogen pada sampel pangan jajanan anak sekolah dasar di Pulau Sapeken, Sumenep, Jawa Timur Ivani Dayanara; Retno Kawuri; Dwi Ariani Yulihastuti
Jurnal Biologi Udayana Vol 23 No 2 (2019): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (762.33 KB) | DOI: 10.24843/JBIOUNUD.2019.v23.i02.p04

Abstract

School children snacks are food that is found and routinely consumed by children in the school environment.Good and healthy food must be free from hazardous and toxic materials, such as microbial contamination,chemicals and other materials. The consumed food must meet the microbial contamination requirementsbased on SNI (Standar Nasional Indonesia) safety testing. Pathogenic bacteria still a serious problem invarious countries including Indonesia, since they are capable of causing foodborne diseases. Sapeken Islandis located in Sapeken Village, Sapeken District, Sumenep Regency, East Java Province. The purpose of thestudy was to determine the presence of pathogenic bacteria in samples of snacks for school children onSapeken Island. The method used was the Most Probable Number (MPN) for Escherichia coli, Test Numberof Bacillus sp., Test Number of Staphyllococcus sp., Identification of Salmonella, and Total Plate Count(TPC). The test results obtained in the MPN of Escherichia coli included SD M (9.9 ± 3.8) MPN / g, SD 5(8.68 ± 2.73) MPN / g, SD IT (5.08 ± 0.66) MPN / g, and SD 4 (4.26 ± 2.44) MPN / g exceed the SNI limit.JURNAL BIOLOGI UDAYANA 23(2): 60-71 P ISSN: 1410-5292 E ISSN: 2599-285661The results of JPT E. coli in snack empek-empek at SD I and SD 2, and JPT E. coli ojek in all elementaryschools is <3 MPN / g. The results of the empek-empek and ojek samples in all elementary schools for alltests with the parameter number Bacillus sp. is <1 x 103, the number of Staphylococcus sp. is <1 x 102,Salmonella identification is negative, TPC is <1 x 105 CFU / g. The conclusion of the study is snack forschool children (empek-empek) and ojek on Sapeken Island does not contain contamination of pathogenicbacteria Escherichia coli, Bacillus sp., Staphylococcus sp., Salmonella identification, and TPC, except forthe JPT E. coli test on empek empek in SD M, SD 5, SD IT and SD 4 that exceed SNI limits.
Potensi Streptomyces sp. dalam menghambat pertumbuhan jamur Colletotrichum acutatum pada cabai merah besar (Capsicum annum L.) secara in vitro Rochmalia Juniarti Putri; Retno Kawuri; Anak Agung Ketut Darmadi; Inna Narayani
Jurnal Biologi Udayana Vol 25 No 2 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i02.p12

Abstract

Red chilli plant (Capsicum annum L.) is one of the most popular vegetable crops in Indonesian society. One of them the pathogens attacks is Colletotrichum acutatum, a fungus causing anthracnose on red chilli. This study aims to determine the existence of Streptomyces sp. bacteria in the rhizosphere of the red chilli plant; the ability of Streptomyces sp. in inhibiting C. acutatum; Minimum Inhibitory Concentration (MIC) of Streptomyces isolates extracts in inhibiting C. acutatum; The Streptomyces isolation was carried out by dilution method using selective meida, namely Yeast Malt Agar. The Dual Culture method was used as an inhibition test between Streptomyces sp. and C. acutatum in vitro. A well diffusion method was used to test the effectiveness of the Streptomyces sp. and MIC filtrate concentration in inhibiting C. acutatum. The data obtained in this study were analyzed with Analysis of Varian (ANOVA) then continued with Duncan Multiple Range Test with 5% significance. Five Streptomyces isolates were found, namely Streptomyces sp.1, Streptomyces sp.2, Streptomyces sp.3, Streptomyces sp.4, and Streptomyces sp.5 in the rhizosphere of healthy C. annum L. plants in Daup Village, Kintamani District, Bangli Regency. Streptomyces sp. isolates. can significantly inhibit the growth of the fungus C. acuatum with inhibitory power ranging from 50.30% to 83.76%, Streptomyces sp.5 isolate was able to provide the highest percentage of inhibition in C. acutatum of 83.76 ± 2.91% with MIC 7% (v/v) with a diameter of 6.40 mm.
RED MOLD RICE (ANGKAK) SEBAGAI MAKANAN TERFERMENTASI DARI CHINA: SUATU KAJIAN PUSTAKA Retno Kawuri
Jurnal Biologi Udayana Vol 17 No 1 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Fermented food was produced in both traditional and modern techniques, to increase fungtional value and taste.  One example is Red Mold Rice (RMR) or Angkak, which is famous fermented rice from China. RMR was made from rice, red fungi Monascus purpureus and Monacolin as metabolit seconder, which believe can reduce Cholesterol dan trigelseride. Oral treatment with Cholestyn (1,2 g/d) can reduce LDL (bad cholesterol) up to 30,9% and triglesirida up to 34,1%, and can also increase HDL (good cholesterol) by 19,9%. Beside Monacolin, RMR also contains Cytrinin (0,2-122 mg/kg) which has a negative impact on functional and structural of human kidney and liver.
Antibacterial potency from the waste of durian rind (Durio zibethinus Murr.) against Propionibacterium acnes that causing acnes Made Mira Pratiwi; Retno Kawuri; I Putu Gede Ardhana
Jurnal Biologi Udayana Vol 23 No 1 (2019): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.749 KB) | DOI: 10.24843/JBIOUNUD.2019.v23.i01.p02

Abstract

One of the things that could cause pathogenesis of acne is the activity of normal flora bacteria on the skin, one of them is the bacteria Propionibacterium acnes. The use of extracts which is derived from severalparts of plant such as banana peel, mangosteen peel, dragon fruit peel, potato peel, crinum lily leaf, Marsh fleabane leaf, soursop leaf, soma leaf, green betle vine leaf, and cacao seed that are known to have antibacterial activity could help in acne healing attempt. This research was conducted at Microbiology Laboratory Faculty of Mathematics and Natural Sciences Udayana University from December 2017 until March 2018.The research was conducted with the intention toknow the appropriate solvent to obtain the compound type of durian rind extract dissolved with three types of solvent (methanol, ethanol, ethyl acetate), to look for the smallest resistor value (MIC) of the extract with a solvent that provides the best inhibitory power, and to know the compound type of the extract with a solvent that provides the best inhibitory power. The method is diffusion wells and phytochemical tests. The data that is obtained in the study were analysed by analysis of variance (ANOVA). In inhibition test against P. acnes, it is known that the durian rind ethyl acetate extract effectively showed the inhibitory effect on the bacteria growth, with MIC value of 1.1%. The compound that is contained in the durian rind ethyl acetate extract is terpenoid, steroid, flavonoid, phenolic, and tannin.
ISOLASI STREPTOMYCES SPP. PADA KAWASAN HUTAN PROVINSI BALI SERTA UJI DAYA HAMBATNYA TERHADAP LIMA STRAIN DIARRHEAGENIC ESCHERICHIA COLI I WAYAN EKA DHARMAWAN; MADE SUSUN PARWANAYONI; RETNO KAWURI
Jurnal Biologi Udayana Vol 13 No 1 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

An exploration study of natural resources soil bacteria antibiotic-producer, Streptomyces spp. was done in two steps. The first step was isolation of Streptomyces and the second involved testing their inhibition activities against five strains diarrheagenic Escherichia coli. Soil samples were collected from ten forest areas in Bali. As many as 55 isolates were collected with various macroscopic dan microscopic characters. Most isolates (eight Streptomyces isolates) were collected from forest area in Penulisan, Kintamani (RTK. 20). The diversities of isolates are influenced by environment condition. All Streptomyces isolated were tested against five strains diarrheagenic Escherichia coli to check antibiotic activity for inhibit growth of E. coli. Streptomycine was used as a control. The result showed that the largest inhibition zones of Streptomyces against E. coli strains EHEC, ETEC, EIEC, EPEC and DAEC were produced by Streptomyces PK5 (48,67 ± 0,58 mm), Streptomyces GAA4 (29,00 ± 2,00 mm), Streptomyces GBK3 (42,67 ± 2,08 mm), Streptomyces SkBB5 (29,00 ± 2,65 mm) and Streptomyces GM3 (33,67 ± 3,21 mm) respectively.
IDENTIFICATION OF VIBRIO BACTERIA CAUSED VIBRIOSIS ON FRESHWATER PRAWN LARVAE (Macrobrachium rosenbergii (de Man)) Ni Wayan Desi Bintari; Retno Kawuri; A. A. Gde Raka Dalem
Jurnal Biologi Udayana Vol 20 No 2 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Freshwater prawn (Macrobrachium rosenbergii (de Man)) farming in Bali needs to be supported by the provisionof healthy prawn larvae. Vibriosis infection can be a limiting factor on larval production system which cause highmass mortality in larvae. Therefore a bacteriological study is very important to identify of Vibrio species whichcan cause vibriosis in freshwater prawn larvae. Screening of Vibrio bacteria carried on larval rearing water atUPT Pembenihan Dinas Kelautan dan Perikanan Provinsi Bali which located in Pesinggahan Village, Klungkung.During study, 5 Vibrio isolates can be characterized (VSP01, VSP02, VSP04, VSP05 and VSP06) which wereisolated as dominant Vibrio bacteria in larval rearing water. Koch postulate test results showed that VSP06 cancause vibriosis in freshwater prawn larvae. The result of identification by BBL Cystal™ Identification Systems andidentification book Bergeys’s Manual of Determinative Bacteriology Ninth Edition (Holt et al., 1994), BL6 wasidentified as Vibrio anguillarum.
TOTAL BAKTERI PADA JAMU TRADISIONAL DI PASAR KEDONGANAN KELURAHAN JIMBARAN KABUPATEN BADUNG PROVINSI BALI Putu Ayu Sukmawati; Meitini W. Proborini; Retno Kawuri
Jurnal Biologi Udayana Vol 16 No 2 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui jenis-jenis cendawan, total koloni bakteri, dan mengetahui keberadaan bakteri pencemar Escherichia coli yang terdapat pada jamu tradisional di Pasar Kedonganan. Sampel diambil dari 4 pedagang jamu, setiap pedagang diambil 4 sampel jamu yaitu jamu beras kencur, sirih, kunyit dan sambiloto. Perhitungan Total koloni cendawan dan total koloni bakteri pada jamu dilakukan dengan menggunakan metode pengenceran dan total koloni bakteri secara statistik dianalisa dengan menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa 6 jenis cendawan yaitu: Aspergillus niger, A. flavus, Penicillium citrinum, P. digitatum, P. brevicompactum, dan Acremonium sp ditemukan pada jamu. Total jumlah koloni cendawan tertinggi ditemukan pada jamu beras kencur (107x105 CFU/ml) sedangkan terendah ditemukan pada jamu kunyit (20,5 x 105 CFU/ml). Batas standar kandungan jamur pada makanan yang direkomendasikan oleh Departemen Kesehatan RI adalah sebesar < 104 CFU/ml. Total bakteri tertinggi ditemukan pada jamu beras kencur (267,6x108 CFU/ml), sedangkan terendah ditemukan pada jamu kunyit (39x108CFU/ml). Kandungan bakteri juga melampaui standar dari Departemen Kesehatan RI Keseluruhan jamu telah diuji telah melampaui ambang batas Departemen Kesehatan RI yaitu sebesar < 106 CFU/ml. E.coli di temukan jamu sambiloto, jamu kunyit, jamu beras kencur.  Oleh karena itu kehati-hatian perlu dilakukan jika meminum jamu.
AKTIVITAS ANTIBAKTERI FILTRAT Streptomyces sp. KCM2 TERHADAP MULTIDRUG RESISTANT Acinetobacter baumannii SECARA IN VITRO NI KADEK LOSIANI; RETNO KAWURI; A. A. KETUT DARMADI
Jurnal Biologi Udayana Vol 21 No 1 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.281 KB) | DOI: 10.24843/JBIOUNUD.2017.vol21.i01.p05

Abstract

Acinetobacter baumannii is one of the pathogen which leads to multidrug resistant (MDR-A baumannii). This pathogen is often causing outbreaks of infections to the patients which are receiving treatment in the intensive care unit in hospital. This study aims to find out the antibacterial activity and the Minimum Inhibitory Concentration (MIC) of Streptomyces sp. KCM2 filtrate in inhibiting the growth of MDR-A baumannii. Streptomyces sp.KCM2 isolates was isolated in rhizosphere of white turmeric (Curcuma mango Val.) (Losiani et al., 2016). MDRA. baumannii isolates were obtained from stock culture in Clinical Microbiology Laboratory Sanglah Hospital in Denpasar. The antibacterial and the MIC test of Streptomyces sp. KCM2 filtrate was using the wells diffusion method. The research design was used Completely Randomized Design (CRD) and the inhibitory zone diameterdata of MIC test were analyzed by Analysis of Varian (ANOVA), and then continued by Duncan Multiple Range Test in significance level 5%. The results of this study showed that the Streptomyces sp. KCM2 filtrate was able inhi biting with diameter zone of 23,44 mm and MIC of filtrate was 4% (v/v) with inhibition zone diameter of 8.77 mm.
PENGARUH pH MEDIA PERTUMBUHAN TERHADAP KETAHANAN DARI Rhizobium sp. PADA TANAH YANG BERSIFAT ASAM Ni Made Widyasari; Retno Kawuri; I Ketut Muksin
Jurnal Biologi Udayana Vol 17 No 2 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.154 KB)

Abstract

Telah dilakukan penelitian yang bertujuan mengetahui ketahanan Rhizobium sp. yang ditumbuhkan pada pH media pertumbuhan 7,0 dan 5,8 serta mengetahui kemampuan hidup Rhizobium sp. pada media tanah asam dengan pH 5,0 dan respon inokulum Rhizobium sp. pada tanaman kedelai secara in vivo. ATR (Acid Tolerance Responce) didapatkan dengan cara menumbuhkan Rhizobium sp. dengan pH media pertumbuhan 7,0 dan ditambahkan dengan media YMB (Yeast Extract Mannitol Broth) dengan pH 5,8 dan pH 7,0, diinkubasi pada suhu 280C selama 24 jam pada shaker. Penghitungan total mikroba dilakukan setiap 2 jam sekali dengan menggunakan platting method. Uji ketahanan Rhizobium sp. pada tanah asam dengan pH 5,0 secara in vitro dengan cara menghitung total bakteri dengan menggunakan platting method setiap hari selama 28 hari. Uji in vivo dilakukan dirumah kaca dengan menggunakan metoda MPN (Most Probable Number). Hasil penelitian menunjukkan Rhizobium sp. yang dikondisikan pertumbuhannya dengan pH 5,8 lebih resisten dan dapat membentuk ATR dibandingkan dengan Rhizobium sp. yang ditumbuhkan pada pH media 7,0. Pada pH 5,8 setelah 10 jam total bakteri 285 CFU/g sedangkan pada pH 7,0 total bakteri 148 CFU/g. Rhizobium sp. mampu hidup pada tanah asam dengan pH 5,0 dan membentuk ATR pada hari ke 6 dengan total bakteri 137 x 104 CFU/g, tetapi respon inokulum Rhizobium sp. pada tanah dengan pH 5,0 tidak terjadi pembentukan nodul pada tanaman kedelai dikarenakan tanaman kedelai mengalami defisiensi unsur hara.
Co-Authors Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andreas Putro Ragil Santoso ANINDA OKTAVIA RAHARINI Aryadi Millenia Saputra Bayu Putri Handayani Chandrika, Carmen Gisela Darmadi, A.A. Ketut Diah Kharismawati Djereng Dwi Ariani Yulihastuti Gusti Ngurah Alit Susanta Wirya Gusti Ngurah Wisnanda Putra H. Yuswanti I Gede Agus Pradana Putra I Gusti Agung Ayu Ratnawati I Ketut Ginantra I KETUT MUKSIN I Komang Adi Widyastama I Made Dira Swantara I Made Sara Wijana I Putu Gede Ardhana I WAYAN EKA DHARMAWAN I Wayan Yogi Widyastana I.G.A. Gunadi I.G.A.S. Wahyuni Ida Ayu Putri Darmawati Ida Bagus Gede Darmayasa Ilham . Inna Narayani IRIANI SETYAWATI Ivani Dayanara Job Nico Subagio K.A. Yuliadhi K.B. Susrusa Khamdan Khalimi Khotima Dwi Cahya Luh Made Ary Somia Vagestini LUH Puru ESWARYANTI KUSUMA YUNI Made Mega Yuliasari Made Mira Pratiwi MADE PHARMAWATI Made Ria Defiani MADE SUSUN PARWANAYONI Meitini Proborini Wahyuni Muhammad Inas Riandi N.N.A. Mayadewi NI KADEK LOSIANI Ni Ketut Febri Antini Ni Ketut Yuliana Sari Ni LUH ARPIWI Ni Luh Cipta Ayumi Nellawati Ni Luh Suriani Ni Luh Watiniasih Ni Made Suartini Ni Made Widyasari Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Niti Rahayu Ni Wayan Desi Bintari Ni Wayan Purni wirathi Ni Wayan Sudatri NURI MANDAN SARI Putri Permata Putu Anjarina Putu Ayu Parwati Putu Ayu Sukmawati Putu Cindy Arista Revi Juniar Nutrihidayah Rindang Dwiyani Risha Masfufah Risky Vidika Apriyanthi Rochmalia Juniarti Putri Rochmalia Juniarti Putri SANG KETUT SUDIRGA SARMILA TASNIM Suta Arta TRISNA AGUNG PHABIOLA WIWIK SUSANAH RITA Y. Fitriani Yan Ramona