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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Jurnal Silvikultur Tropika (JST) Seminar Nasional Aplikasi Teknologi Informasi (SNATI) Agrotrop : Journal on Agriculture Science Jurnal Agrista JIK Jurnal Ilmu Komputer Jurnal Keteknikan Pertanian Cropsaver : Journal of Plant Protection Jurnal Algoritma LISANIA Jurnal Ilmu dan Pendidikan Bahasa Arab Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains OPSI AGRIVITA, Journal of Agricultural Science Buletin Kebun Raya agriTECH JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Kebijakan dan Pelayanan Publik Law and Justice Jurnal Matematika Sains dan Teknologi Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Teknik Informatika STMIK Antar Bangsa Jurnal Ergonomi dan K3 Jurnal Agrotek Tropika Jurnal Pengabdian Masyarakat AbdiMas JURNAL PERTANIAN PRESISI (JOURNAL OF PRECISION AGRICULTURE) Journal of Tropical Crop Science Jurnal Ilmiah Pendidikan Scholastic JUMLAHKU: Jurnal Matematika Ilmiah STKIP Muhammadiyah Kuningan WIDYA LAKSANA Ensiklopedia of Journal Molekul: Jurnal Ilmiah Kimia Menara Perkebunan Jurnal Bidan Cerdas Jurnal Sains dan Teknologi Reaksi Jurnal Kebidanan Mutiara Mahakam Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Jurnal Sains Agro Jurnal ABDIKU Indian Journal of Forensic Medicine & Toxicology Indonesian Science Education Research (ISER) Jurnal Konstitusi Jurnal Teknologi Kimia Unimal Jurnal Algoritma Jurnal Penelitian Kehutanan Wallacea
Nurul Khumaida
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti Kampus IPB Darmaga, Bogor 16680, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Nursing Physics Public Health Social Sciences Other

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Induksi dan Proliferasi Kalus Embriogenik pada Beberapa Genotipe Kedelai Nurul Khumaida; Tri Handayani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.999 KB) | DOI: 10.24831/jai.v38i1.1681

Abstract

The successful of gene transformation on crop plants depends on the ability of explant to develop somatic embryos. The effect of medium composition on induction and proliferation of embryonic callus was analyzed on several soybean genotypes, including low irradiance (LI) tolerant genotype (Ceneng, Pangrango C6-30-10 and C6-76-10) and two LI sensitive genotypes (Godeg and Slamet). Immature cotyledons (14 DAA) were cultured in induction medium including MSIA (MS, vitamin B5, 30% sucrose, 0.2% gelrite, 10 mg L-1 2,4-D and 10 mg L-1 NAA) and MSIB (MS, vitamin B5, 30% sucrose, 0.2% gelrite, and 40 mg L-1 2,4-D). Embryonic calli was subcultered one month after initiation onto proliferation medium including MSIIA (MS, vit. B5, 30% sucrose, 0.2% gelrite, 5 mg L-1 2,4-D and 5 mg L-1 NAA) and MSIIB (MS, vitamin B5, 30% sucrose, 0.2% gelrite, and 20 mg L-1 2,4-D). The result showed that percentage of callused explant was 76-94% and the highest initiation obtained on Pangrango genotypes. The average of calli diameter at 1 month after initiation was 0.5-1.2 cm. Calli which was obtained on MSIA medium showed yellow, tranparent, and friable, whereas calli was obtained on MSIB medium showed yellow-brown, transparent, and friable. Increasing calli diameter and structure were obtained on proliferation medium. The combination of 2,4-D and NAA on MSIA and MSIIA both are induction and proliferation medium respectively were better than MSIB and MSIIB which were contain only 2,4-D. Ceneng genotype showed best performance of somatic embryogenesis than others, and Slamet genotype showed lowest response both on callus induction and proliferation.   Keywords: immature cotyledons, embryonic callus, NAA, 2,4
Characterization of Full Length Sequence of JJ3 Isolated from Shade Tolerant Soybean Nurul Khumaida; , Kisman; Didy Sopandie
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 2 (2008): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.794 KB) | DOI: 10.24831/jai.v36i2.13696

Abstract

The aim of this study was to characterize full length sequence of JJ3 isolated from shade tolerant soybean. The characterization of full length sequence involved analyses of structure of full length sequence, open reading frame (ORF) or coding sequence (CDS), and homology. The analyses of JJ3 full length sequence structure were done using GeneScanW program, coding sequence (CDS) or open reading frame (ORF) using ORF Finder program, and the homology of JJ3 full length sequence were done using BLAST and clustalW programs. Results showed that the full length sequence of JJ3 consisted of single exon and polyA. Single exon was a coding sequence (CDS) 633 bp produced open reading frame (ORF) with 210 amino acid deduction comprised of 136 conserved amino acids and 77 varied amino acids. The conserved domain of JJ3 produced functional protein, PsaD photosystem I (PSI) subunit, related to photosynthetic transport electron in PSI reaction center. The full length of JJ3 homolog to psaD, a gene encoding protein subunit photosystem I (PSI) related to mechanism of plant adaptation to shade stress, in wood tobacco (Nicotiana sylvestris), rice (Oryza sativa), tomato (Lycopersicon esculentum), barley (Hordeum vulgare), spinach (Spinacia oleracea), Arabidopsis (Arabidopsis thaliana), and potato (Solanum tuberosum).Key words: full length sequence, gene, homology, shade tolerant, soybean
Co-Authors , Kisman . Wusnah A. Sofwan Achmad . ADI SETIADI Agus Purwito Agustina, Ferra Anggita AHMAD JUNAEDI Ahmad Rifqi Fauzi Ali Djamhuri Almia Permata Putri Amril Aman Andi Dahliaty Aniversari Apriana Aniversari Apriana Aniversari Apriana Ardana, I. N. Kutha Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Aris Purwanto Arrin Rosmala Atmitri Sisharmini Atmitri Sisharmini Atmitri Sisharmini Bambang Sapta Purwoko Bhayu Hartanti Bintang, dan Maria Cakranegara, Pandu Adi Cucu Sukmana, Cucu Danar Dono Dede J. Sudrajat Dede J. Sudrajat Dede J. Sudrajat Dede Jajat Sudrajat Dede Kurniadi DEWI SARTIAMI Dewi Sukma Dewi Sukma Didy Sopandie Diny Dinarti Dwi Pratiwi Edi Santosa Efendi, Darda Emmy Darmawati Endah Yulia Enny Sudarmonowati Evi Fitriany Faqih Udin Firdausya, Andina Fabrini Freddy Tua Musa Panggabean Gustaaf A Wattimena, Gustaaf A Gustaaf A. Wattimena Gustaaf Adolf Wattimena Gustaaf Adolf Wattimena HADIPOENTYANTI, ENDANG Hartati, RR. Sri Heldiyanti, Rina Hendri Hermawan Adinugraha HUSNI, ALI I Ismayani I MADE ARTIKA Ida Duma Riris Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Roostika Ika Roostika Ika Roostika Indrastuti Apri Rumanti Irdika Mansur Irfan MARTIANSYAH Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Iswari S Dewi Iswari Saraswati Dewi Jalaluddin Jalaluddin Kartika Ning Tyas Kartika Ning Tyas Kasutjianingati . Kisman Kisman Kurniawan R. Trijatmiko Kurniawan Rudi Trijatmiko Kurniawan Rudi Trijatmiko Lefin Kafindra Lestari, Risza Putri Lisnawaty Simatupang Lola Adres Yanti Lukman Hakim M Murtadha M. Amir M. Asyabul Zikki Maemonah, Maemonah MARIA BINTANG Marini Damanik Mariska, Ika Maryati Evivani Doloksaribu MEYNARTI SARI DEWI IBRAHIM MEYNARTI SARI DEWI IBRAHIM Muaz Adbdul Karim Muaz Adbdul Karim Muhamad Syukur Muhammad Irfan Habibi MUHAMMAD SYUKUR Mulyatno, Mulyatno Munif Ghulamahdi N. Sri i Hartati Nafilawati wa ode Nata Suharta Natalini Nova Kristina Natalini Nova Kristina Nataniel Tandirogang Nugroho, Candra Catur Nura ,, Nura Nurbaiti Nurbaiti Nurul Fatimah Nurul Fauziah O K Sofyan OTIH ROSTIANA OTIH ROSTIANA Pienyani Rosawanti Rahmi Henda Yani Rasyad, M. Ashadi Rina Hapsari Wening, Rina Hapsari Rinda Cahyana Riyanti Catrina H S Riza Arief Putranto Rizkiannur Putri, Amalia Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rozanna Dewi Ryan Budi Setiawan Sadewi Maharani Sadewi Maharani, Sadewi Saepudin, Adam Safitri Safitri Saronom S ilaban Sarwono Hardjowigeno Shafwan Fahmi Silawati, Tutisiana Silawati Tutisiana Sintho Wahyuning Ardie Siswati, Leni Siti Aisyah Siti Kurniawati Slamet Budijanto Slamet Susanto Sri Enny Triwidiastuti Sri Helianty Sry Rahmadani Subekti, Isnani Sudarsono Sudarsono Sudarsono Suhesti, Sri Sukma, Dewi Sulastri Sulastri Sulhatun Sulhatun SUPRIADI SUPRIADI Suryati Suryati Suryo Wiyono Sutalaksana, Iftikar Z Sutrisno Mardjan Sutrisno, Sutrisno Suyanti Kasimin Syamsul Bahri Syamsul Bahri Tarigan, Asmara Iriani Tengku Mia Rahmiati Teti Arabia Thifany, Ariny Jihan Tri Handayani Tri Joko Santoso Tri Lestari Mardiningsih Tri Lestari Mardiningsih, Tri Lestari Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas Trismi Ristyowati Ulfah J. Siregar Ulfah Juniarti Siregar Ummu Kalsum Wahyuning Ardie, dan Sintho Waizul Fahri Purba Waras Nurcholis Warid Warid Wattimena, and Gustaaf Adolf WATTIMENA, G. A. Wening Enggarini Wening Enggarini Widiatmaka Widodo , Widowati, Sartika Widya Hartati, Widya Wijayanti, Mustika Eka Willy Bayuardi Suwarno Yudiansyah Yudiansyah Yundari, Yundari Yunita, Rossa Zuli Nuraeni