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All Journal AQUATIC SCIENCE & MANAGEMENT JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Matematika & Sains AGRI-SOSIOEKONOMI EKOTON
Markus T. Lasut
Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi, Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Mathematics Social Sciences

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Kepekaan Cacing Laut Ophryotrocha diadema (Polychaeta: Dorvilleidae) terhadap Cemaran Metil Merkuri (MeHg) Markus Talintukan Lasut; Henneke Pangkey
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2594

Abstract

Susceptibility of the marine polychaete Ophryotrocha diadema (Polychaeta: Dorvilleidae) towards the neurotoxic methyl mercury (MeHg) contamination was studied in an experimental chamber, which was aimed to assess and compare the susceptibility level of the organism based on its generations (F0, F1, F2, and F3). Seven variables of growth and reproduction aspects were applied as indicators in this study; they were: 1) individual growth, 2) first time the egg laid, 3) number of eggs per individu, 4) number of eggs per egg mass, 5) number of eggs to larva per egg mass, 6) number of mortality per egg mass, and 7) reproductive potential. Observation was conducted on the treatment (MeHg in concentration of 0,00025 ppb) and the control (no MeHg) to each of the generations (F0, F1, F2, and F3). Data obtained were analysed for average and standard deviation. Comparison of susceptibility within the generations was calculated using the variable of reproductive potential. The results showed that there were differences between the treatments and the control for all of the variables. Comparison on the susceptibility of the polychaete within the generations to MeHg contamination was F0<F1=F3<F2. It was concluded that the F2 generation had the highest susceptibility among the others.
Predatorism and Cannibalism of Fish Betutu (Oxyeleotris marmorata Blkr.) In Lake Tondano, Minahasa regency, North Sulawesi Rama Presley Kambey; Rose O.S.E. Mantiri; Markus T. Lasut
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.23217

Abstract

Aims of this study is to determine the ability of marble goby to prey other fish and other marble goby in Tondano Lake. This research was conducted in August 2017 to December 2017. Measure of aquarium used as container is 120 cm x 40 cm x 40 cm which was divided into 2 parts, equipped with a camera in addition to observe the reaction of predation on marble goby. To know the predation behaviour of marble goby, using sample size is <15 cm and> 15 cm, and the prey fish is used was silver barb (Puntius javanicus) size is 10 cm - 20 cm. To determine the cannibal behaviour is used marble goby size is <15 cm and> 20 cm, and prey another marble goby size is 10-20 cm. The results showed the fastest predatorism reaction of marble goby attack silver barb occurred is 6 minutes after treatment, and the fastest cannibalism reaction of marble goby occurred after 45 minutes.Keywords: Betutu, Lake Tondano, Cannibalism, Predation, Predatorism. ABSTRAKPenelitian ini bertujuan untuk mengetahui kemampuan ikan betutu dalam memangsa ikan lain dan sesama ikan betutu di Danau Tondano. Waktu penelitian dilakukan pada bulan Agustus 2017 sampai Desember 2017. Wadah yang digunakan adalah akuarium berukuran 120 cm x 40 cm x 40 cm yang dibagi menjadi 2 bagian dengan menggunakan sekat, dilengkapi dengan kamera untuk mengamati reaksi pemangsaan ikan betutu. Untuk mengetahui sifat predator ikan betutu digunakan sampel berukuran <15 cm dan >15 cm, dan mangsa yang digunakan adalah ikan tawes (Puntius javanicus) yang berukuran 10 cm - 20 cm. Untuk mengetahui sifat kanibal ikan betutu digunakan ikan betutu berukuran <15 cm dan >20 cm, dan mangsa ikan betutu berukuran 10-20 cm. Hasil penelitian menunjukkan reaksi tercepat predatorisme ikan betutu terhadap ikan tawes terjadi setelah 6 menit setelah perlakuan, dan reaksi tercepat kanibalisme ikan betutu terjadi setelah 45 menit.Kata kunci: Betutu, Danau Tondano, Kanibalisme, Pemangsaan, Predatorisme
In Vitro Culture of Seaweed Kappaphycus alvarezii under Different Formulation of Growth Stimulating Substances and Culture Media Mega D. Dalero; Grevo S. Gerung; Edwin L.A. Ngangi; Lawrence J.L. Lumingas; Markus T. Lasut
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.23375

Abstract

This study aims at obtaining a sustainably superior seed stock following the characteristics of the parent plant, determining the best formulation of the growth stimulating substance. In general, cytokinin and auxin combination was used, but this study also added with the combination of cytokinin and giberelin and cytokinin and abscisic acid (AA).Parameters measured were bud length, number of buds, and survival rate. Bacterial Vibrio sp test was also done as a cause of the explant mortality. Results showed that the longest bud was recorded in treatment C (S+A 1:2.5) cultured in a jar, 1.343 mm long, 38% of survival, while the highest number of buds was found in treatment B (S+A 1 : 2) 8.86. The shortest bud was recorded in treatment J (S + AA 1:2.5) cultured in a jar, 0.093 mm long, 2.64 buds, 10% of survival, while the explant cultured in the bottle had a length of 0.051 mm long, 1.50 buds, 4% of survival. As conclusion, the best growth stimulating substance was found in the treatment C for the bud length and the survival rate, while the best number of bud was recorded in the treatment B. The best culture tank was topless bottle (aerated). In vitro culture could also use S + G formulation. The explant mortality was caused by Vibrio charchariae. The use of S + AA formulation had lower growth than that of control treatment.Keywords :in vitro, growth stimulating substance, culture media, Kappaphycus alvarezii, Vibrio charchariae ABSTRAKPenelitian ini bertujuan untuk memperoleh benih unggul secara berkelanjutan yang mengikuti karakteristik dari tanaman induk, menentukan formulasi terbaik dari substansi pertumbuhan merangsang. Secara umum, kombinasi sitokinin dan auksin digunakan, tetapi penelitian ini juga menambahkankombinasi sitokinin, giberelin, sitokinin dan asam absisat (AA). Parameter yang diukur adalah panjang tunas, jumlah tunas, dan tingkat kelangsungan hidup. Bakteri Vibrio Uji sp juga dilakukan sebagai penyebab kematian eksplan . Hasil penelitian menunjukkan bahwa tunas terpanjang terdapat pada perlakuan C (S + A 1: 2,5)  kultur dalam toples, 1,343 mm, 38% hidup, sementara jumlah tertinggi tunas ditemukan pada perlakuan B (S + A 1: 2) 8.86 . Jumlah tunas paling sedikit terdapat pada perlakuan J (S + AA 1: 2,5) yang dikultur dalam toples, 0,093 mm, 2,64 tunas, 10% hidup, sedangkan eksplan yang dikultur dalam botol memiliki panjang 0.051 mm, 1. 50 tunas , 4% bertahan hidup. Sebagai kesimpulan, pertumbuhan terbaik merangsang zat ditemukan dalam perlakuan C untuk panjang tunas dan tingkat kelangsungan hidup, sementara jumlah tunas terbanyak ditemukan pada perlakuan B. Penggunaan wadah budaya terbaik adalah topless yang diaerasi. Kultur in vitro juga dapat menggunakan formulasi S + G. Kematian eksplan disebabkan oleh Vibrio charchariae . Penggunaan formulasi S + AA memiliki pertumbuhan yang lebih rendah dari pada pengobatan kontrol .Kata kunci : in vitro, zat perangsang tumbuh, media kultur, Kappaphycus alvarezii, Vibrio charchariae
Geographic Information System Applications for BeachTourism Area Determination in Bitung City J. Ch. Kumaat; Markus T. Lasut; Adnan S. Wantasen
Jurnal Ilmiah PLATAX Vol. 5 No. 1 (2017): ISSUE JANUARY - JUNE 2017
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.5.1.2017.14968

Abstract

This research was conducted with the aim to determine the suitability of beach tourism area in Bitung city by using Geographic Information System application. This information is necessary to support the provision of geospatial information the beach, so the tourist management in research into more optimum location. Suitability Analysis Zone (spatial) analysis of the suitability of the area is done by using Geographic Information System (GIS), a computer-based geospatial information system involving Arc GIS software 10.1. Based on analysis of the suitability of the area spatially, shows that, for beach tourism area in the area of research, can be divided into 4 (four) classes, namely: (1) land suitability classes by category S1 (Very appropriate) with an area of 376.76 ha (5.87%); (2) land suitability classes with category S2 (Subject) with an area of 262.97 ha (4:10%) (3) land suitability classes with category S3 (In accordance marginal) with an area of 640.48 ha (9.99%) and (4) land suitability classes categories N (Not available) with an area of 5133.79 ha (80.04%) Keywords: Suitability, Area, Zone, Beach   Abstrak Penelitian ini dilaksanakan dengan tujuan untuk menentukan kesesuaian kawasan wisata pantai di Kota Bitung dengan menggunakan aplikasi Sistem Informasi Geografis. Informasi ini sangat diperlukan untuk mendukung penyediaan informasi geospasial pantai, sehingga pengelolaan wisata di lokasi penelitian menjadi lebih optimum.  Analisis Kesesuaian Kawasan (Spasial) Analisis kesesuaian kawasan dilakukan dengan menggunakan Sistem Informasi Geografis (SIG), yaitu sistem informasi geospasial berbasis komputer dengan melibatkan perangkat lunak Arc GIS 10.1. Berdasarkan hasil analisis kesesuaian kawasan secara spasial, diperoleh bahwa untuk kawasan wisata pantai pada daerah penelitian, dapat dibagi menjadi 4 (empat) kelas, yaitu: (1) kelas kesesuaian lahan dengan kategori S1 (Sangat Sesuai) dengan areal seluas 376.76 ha (5.87%); (2) kelas kesesuaian lahan dengan kategori S2 (Sesuai) dengan areal seluas 262.97 ha (4.10 %) (3) kelas kesesuaian lahan dengan kategori S3 (Sesuai Marginal) dengan areal seluas 640.48 ha (9.99%) dan (4) kelas kesesuaian lahan dengan kategori N (Tidak Sesuai) dengan areal seluas 5133.79 ha (80.04%)
A study on management of mangrove and the knowledge of local community in Bahoi of West Likupang Subdistrict of North Minahasa District Vonne Lumenta; Stephanus V. Mandagi; Markus T. Lasut
Jurnal Ilmiah PLATAX Vol. 5 No. 1 (2017): ISSUE JANUARY - JUNE 2017
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.5.1.2017.14970

Abstract

A study on community based mangrove management was conducted in Bahoi of North Minahasa District of North Sulawesi Province of Indonesia. This aims of the study were to examine the management of mangrove including community involvement in the whole processes as well as the institutional settings; to examine knowledge and atitute of the community of Bahoi toward the management processes; to find out its impacts to the community and marine ecosystems.   Methods used in this study were interviews and surveys. For the former, all key persons involving in the management including representative of government were interviewed. With the latter, 30 community members or around 10% of total population were randomly selected and requested to fill in questionnaries containing multiple choices questions to meet the objectives of the study. This study revealed that the management of mangrove has been projects driven activities since year 2000. Yet communities were partly involved in the management including during the establishment of organization and village Ordinance, the survey shows that only 30% of respondents actively involved. That is why 63% of respondent argue that the management processes is lacking and 23% recon that it should be improved. Moreover, 100% of respondents claim that they strongly support conservation of mangrove and other coastal resources; 90% of the respondent answer that cultural background (Sangiran ethnicity) drives their attitude about preserving the coastal resources. In terms of implication of the management mangrove and other coastal resources in Bahoi, they argue that it has resulted in improvement of income and a healthy mangrove ecosystem. Keywords: Mangrove, Management, Bahoi     Abstract Penelitian ini tentang pengelolaan mangrove berbasis masyarakat telah dilakukan di Desa Bahoi di Kabupaten Minahasa Utara Propinsi Sulawesi Utara Indonesia. Tujuan penelitian ini untuk mengetahui proses dan dinamika pengelolaan mangrove berbasis masyarakat khususnya tentang keterlibatan masyarakat dan faktor-faktor yang mempengaruhi pengelolaan terutama pengetahuan dan sikap masyarakat, serta dampak pengelolaan terhadap masyarakat dan ekosistem pesisir lainnya. Teknik pengumpulan data yang digunakan adalah wawancara (interview) dan survei. Wawanara telah dilakukan terhadap semua tokoh kunci yang terlibat dan mempengaruhi pengelolaan, sedangkan untuk survei dengan menggunakan kuisioner, sejumlah 30 responden atau sekitar 10% dari jumlah penduduk telah dipilih secara random bersedia memberikan jawaban atas pertanyaan-pertanyaan pilihan berganda (multiple choises). Penelitian ini mengdapatkan bahwa pengelolaan mangrove di Desa Bahoi didorong oleh proyek pemerintah sejak tahun 2000. Namun masyarakat belum seluruhnya dalam proses pengelolaan mangrove termasuk dalam pembuatan lembaga dan Peraturan Desa tentang pengelolaan sumberdaya pesisir. Makanya 63% responden mengakui kalau pengelolaan yang ada kurang baik dan 23 % menyarankan perlu perbaikan. Selanjutnya 100% menyatakan mendukung sepenuhnya usaha konservasi mangrove dan sumberdaya pesisir lain. 90 % dari mereka percaya bahwa factor budaya Sangir yang mendorong sikap mereka untuk menjaga lingkungan pesisir. Mengenai dampak pengelolaan ekosistem mangrove, masyarakat dan pemerintah menjawab bahwa telah membantu meningkatkan pendapatan atau ekonomi masyarakat dan ekosistem mangrove semakin sehat. Kata kunci: Mangrove, Managemen, Bahoi
Predation Intensity in Mangrove Ecosystem in Marine Protected Area, North Sulawesi Tabita S.H. Suyoto; Farnis B. Boneka; Nego E. Bataragoa; Sebastian C. A. Ferse; Lawrence J. L. Lumingas; Markus T. Lasut; Deiske A. Sumila; Edwin L. A. Ngangi
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.24415

Abstract

This study aims to get an overview of the intensity of predation on mangrove ecosystem in five marine protected areas (MPA), namely Tumbak, Basaan, Blongko, Bahoi and Tambun. The research method was carried out by installing Squidpops bait within one hour and calculating the number of lost bait during the exposure of baits in high tide. Fish species that migrate in the mangrove area are obtained through visual census; Mega Bentos is recorded.  The result of this study indicates the intensity of predation in the mangrove ecosystem in the five North Sulawesi DPLs are varied in each location, which has the possibility of being influenced by local condition, predatory fish population, the level of disturbance at observation, method and level of preference for the bait provided.Keywords: Predation, Predator, Mangrove, Fish Community, Squidpops ABSTRAKPenelitian ini bertujuan untuk mendapatkan gambaran intensitas predasi pada ekosistem mangrove di lima daerah perlindungan laut (DPL), yaitu Tumbak, Basaan, Blongko, Bahoi dan Tambun. Metode penelitian dilakukan dengan pemasangan umpan Squidpops dalam waktu 1 jam dan menghitung jumlah umpan yang hilang selama umpan terpapar pada saat air pasang. Jenis ikan yang bermigrasi di daerah mangrove diperoleh melalui sensus visual; mega bentos dicatat. Hasil dari penelitian ini menunjukkan intensitas predasi di ekosistem mangrove pada 5 DPL Sulawesi Utara bervariasi pada tiap lokasi yang memiliki kemungkinan dipengaruhi oleh kondisi lokal, populasi ikan predator, tingkat gangguan saat pengamatan, metode dan tingkat kesukaan pada umpan yang disediakan.Kata Kunci: Predasi, Predator-Mangsa, Mangrove, Komunitas Ikan, Squidpops
ENDAPAN MERKURI (Hg) DI MUARA SUNGAI KIMA DAN TALAWAAN DARI KEGIATAN PERTAMBANGAN EMAS RAKYAT DI KABUPATEN MINAHASA UTARA Markus T. Lasut
EKOTON Vol. 3 No. 2 (2014): Juli - Desember 2014
Publisher : PPLH-SDA, Lembaga Penelitian Unsrat Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mercury (Hg) discharge through the artisanal gold mining activities is one of the environmental concerns due to its emission that may follows natural methylation of mercury to result environmental contamination with an impact in human health. To assess Hg deposit from such activities, sampling was carried out in a watershed area of Talawaan, North Minahasa Regency, where that type of mining are widely occurred. The sampling was focused at the river mouth of Kima and Talawaan inside the watershed. Moisture concent (MC) and total mercury (THg) on dry weight basis were measured in the sampled sediments. The results show that the MC of the samples in both rivers varied based on their sampling points; the average was 0.30 (30%) in Kima and 0.25 (25%) in Talawaan. The concentration of THg found in sediment of Kima river mouth was uniform from one point to the others. Conversely, it was forming a gradient in the Talawaan where the concentrations decreased at the points close to the beach. In Kima river mouth, the lowest and the highest THg concentrations were found at Point B and A, respectively; while its average concentration was 0.871 mg/kg. In Talawaan river mouth, the lowest and the highest concentration of THg were found at Point B and A, respectively. The average concentration of THg in this area was 0.383 mgkg. Comparing the results within the areas, the concentration of THg found at Talawaan river mouth was higher than Kima.
Mitigation of marine debris from land: Formulation of the best options for managing marine debris in the coastal city of Manado, Indonesia Warouw, Veibe; Mamuaja, Jane M.; Pane, Lindon R.; Maramis, Regina U.; Lasut, Markus T.
AQUATIC SCIENCE & MANAGEMENT Vol. 11 No. 2 (2023): OCTOBER
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.v11i2.54382

Abstract

Mitigation of marine debris in Manado City, the city which is situation in front of the Manado bay, is very important in order to prevent its effect on the coastal communities and as well as for the aesthetics point of view. That activities can directly protect the ecosystem of Bunaken Marine Park (BMP), and can become an important part of the marine waste management activities especially in supporting the commitment of Indonesian government to reducing plastic waste in the sea by 70% by 2025. For this reason, a marine waste management is needed and it should be prepared based on the academic study that are comprehensive and holistic as well as effective and efficient, and also suitable for environmental condition in Manado City. The study was aimed to formulate best options for the existing marine waste management in Manado City. By using qualitative-descriptive and content-analysis methods, four aspects of managemet were studied, and they are 1) public awareness, 2) institutional arrangements, 3) regulations, policies and management plans, and 4) waste management guidelines. The data obtained were quantitatively and qualitatively analyzed. The public awarenes was qualitatively analyzed by using two indicators, community concern and community preference. The existing marine waste management was presented based on studied aspects and the best option was proposed as recommendation for marine waste management in Manado City.
Current condition of shallow-water benthic foraminifera in Manado bay, Indonesia Mamuaja, Jane M.; Mandagi, Stephanus V.; Lasut, Markus T.
AQUATIC SCIENCE & MANAGEMENT Vol. 11 No. 1 (2023): APRIL
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.v11i1.56113

Abstract

Coral reef community in Manado Bay is under pressure due to human activities in areas in and around Manado. In order to be able to wisely manage the bay area and the coral reefs in it, information about present conditions is needed. The use of marine organisms as bioindicators is one way to find information about the condition, and organisms such as foraminifera have the potential to be used as some species share the same requirement for water quality as the corals. Sampling for the foraminifera was carried out at 10 locations, and the depth of water ranged from 2.5 m to 7.0 m. The samples obtained were washed through a 63 μm sieve and dried. The separation of foraminifera tests from other sediments was done under a stereomicroscope and they were then identified to genus level. A total of 40 genera was identified among 3194 specimens. To get the FoRAM Index value, the foraminifera was categorized into three functional groups. 8 genera were categorized as symbiont-bearing group, 8 as opportunistic group, and 29 as heterotrophic group. FoRAM Index was found to be varied from 2.06 to 9.19 which indicated that Manado Bay water condition is also varied among the sampling area. In general based on the data obtained, it is assumed that water in Manado Bay is conducive for coral reef growth.
Zoochemical Analysis and Antibacterial Potential of Starfish, Linckia laevigata Extract Mengko, Christian; Lintang, Rosita A.J; Losung, Fitje; Angkouw, Esther Dellayani; Lasut, Markus T.
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.48927

Abstract

Starfish is one of the marine biota that produces bioactive compounds and has biological activity. The aims of this study were to determine the content of bioactive compounds and to test the antibacterial activity of the extract of the starfish Linckia laevigata against Staphylococcus aureus and Escherichia coli bacteria. Determination of the content of bioactive compounds was carried out by qualitative zoochemical analysis while the antibacterial test used the disc diffusion method. The results showed that the extract of the starfish L. laevigata has bioactive compounds from the alkaloid, triterpenoid, tannin, flavonoid, phenolic, and saponin groups. Inhibitory activity against S. aureus and E.coli based on the inhibition zone formed showed that L. laevigata starfish extract produced low antibacterial activity Keywords : Starfish (Linckia laevigata), Zoochemistry, Antibacterial, Disc diffusion Abstrak Bintang laut merupakan salah satu biota laut yang memproduksi senyawa bioaktif dan memiliki aktivitas biologis.Tujuan penelitian ini adalah mengetahui kandungan senyawa bioaktif dan melakukan pengujian aktivitas antibakteri dari ekstrak bintang laut Linckia laevigata terhadap bakteri Staphylococcus aureus dan Escherichia coli. Penentuan kandungan senyawa bioaktif dilakukan dengan analisis zookimia kualitatif sedangkan pengujian antibakteri menggunakan metode difusi cakram. Hasil penelitian memperlihatkan bahwa ekstrak bintang laut L. laevigata memiliki senyawa bioaktif dari golongan alkaloid, triterpenoid, tanin, flavonoid, fenolik, dan saponin. Aktivitas penghambatan terhadap S. aureus dan E.coli berdasarkan zona hambat yang terbentuk menunjukkan bahwa ekstrak bintang laut L. laevigata menghasilkan aktivitas antibakteri yang rendah Kata kunci :Bintang Laut Linckia laevigata, Zookimia, Antibakteri, Difusi cakram.
Co-Authors Adianse Tarigan Adnan S. Wantasen Adnan Wantasen Alen N. Narasiang Alfret Luasunaung Angkouw, Esther D. Angkouw, Esther Dellayani Bara, Robert. A. Bessie, Donny Mercys Boneka, Farnis B. Boneka Darmono, Oktaviano P. Deiske A. Sumila Deiske Adeliene Sumilat, Deiske Adeliene Deysi Tampongangoy Deysy M. Puansalaing Eddy Mantjoro Edwin D Ngangi Edwin L. A. Ngangi Edwin L.A. Ngangi Emil Reppie Erly Y. Kaligis, Erly Y. Farnis B. Boneka Feni S. Mnsen, Feni S. Fitje Losung Grevo S. Gerung Gustaf Mamangkey Henneke Pangkey Henneke Pangkey Henry E. Lasut, Henry E. Indri Manembu Inneke F. M Rumengan J. Ch. Kumaat Janny D. Kusen Johnny Budiman Joshian N.W. Schaduw Kalalembang, Delarosa Kalebos, Roosa C. Kamagi, Jongky W.A. Karwur, Denny B.A Kawung, Nikita Kumampung, Deislie R. H. Lano, Inayati H.G.M Lawrence J. L. Lumingas Lawrence J.L Lumingas Lawrence J.L. Lumingas Lindon R Pane Lintang, Rosita A.J Lintang, Rosita A.J. Lintang, Rosita AJ Mahale, Moch Machtino A Maitindom, Frits A Makapedua, Daisy M. Malinggas, Christin R.M Mamonto, Rofenly Mamuaja, Jane M. Mandagi, Stephanus V. Mantiri, Desy M. H Manumpil, Abraham W Manumpil, Silvana Maramis, Regina U. Maramis, Regina Urai Mega D. Dalero Mengko, Christian Najib Hi Talib Natalie D Rumampuk Natalie Rumampuk, Natalie Nego E. Bataragoa Nickson J. Kawung, Nickson J. Nikson J. Kawung Pane, Lindon R. Paulus, James Rama Presley Kambey Ramli A. Ismail, Ramli A. Reiny A. Tumbol Roike Iwan Montolalu Rompas, Margresye D. Rompas, Rizald Rondonuwu, Synthia I Ronoko, Stephen R Rose O. S. E. Mantiri, Rose O. S. E. Rose O.S.E. Mantiri Rumampuk, Natalie D.C S. Berhimpon Sadue, Andrew M. Sandra Tilaar Sarif Hidayat, Sarif Sebastian C. A. Ferse Siegfried Berhimpon Sri Yuningsih Noor Stephanus V Mandagi Stephanus V. Mandagi Suawa, Youdy R Sulthana Samad Suria Darwisito, Suria Suzanne L Undap Tabita S.H. Suyoto Trine Sumampouw Undap, Suzanne J Veibe Warouw Vonne Lumenta Wilmy E Pelle Yundari, Yundari Zebblon, Passion Ch.