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All Journal Jurnal Ilmu Pertanian Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Kesehatan Andalas Jurnal Teknik Sipil BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Kimia Terapan Indonesia ANNALES BOGORIENSES OLDI (Oseanologi dan Limnologi di Indonesia) Marine Research in Indonesia Jurnal Riset Biologi dan Aplikasinya Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Annales Bogorienses
PUSPITA LISDIYANTI
Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Editor's Preface AB Vol 10 No 1 (2005) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 10, No 1 (2005): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.188 KB) | DOI: 10.14203/ann.bogor.2005.v10.n1.%p

Abstract

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Guide for Authors AB Vol 17 No 2 (2013) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.31 KB) | DOI: 10.14203/ann.bogor.2013.v17.n2.%p

Abstract

Editorial Boards AB Vol 14 No 2 (2010) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2543.026 KB) | DOI: 10.14203/ann.bogor.2010.v14.n2.%p

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Editorial Boards AB Vol 9 No 1 (2004) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 9, No 1 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.153 KB) | DOI: 10.14203/ann.bogor.2004.v9.n1.%p

Abstract

Editorial Boards AB Vol 13 No 1 (2009) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9.224 KB) | DOI: 10.14203/ann.bogor.2009.v13.n1.%p

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Front Cover AB Vol 17 No 1 (2013) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (778.028 KB) | DOI: 10.14203/ann.bogor.2013.v17.n1.%p

Abstract

Purifikasi Inhibitor Atpase/Rna Helikase Virus Japanese Encephalitis Dari Streptomyces chartreusis Elfita, Lina; Ratnakomala, Shanti; Suryadi, Herman; Lisdiyanti, Puspita; Utama, Andi
Majalah Ilmu Kefarmasian Vol. 6, No. 2
Publisher : UI Scholars Hub

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Abstract

Japanese encephalitis virus (JEV) is a neuropathogenic virus commonly caused cen-tral nervous diseases such as meningitis and severe encephalitis. Although vaccine has been developed, no specific and effective drug is available so far. We previously carried out a screening of inhibitor of JEV RNA helicase, an enzyme that essential for virus replication, from Actinomycetes and found that Streptomyces chartreusis produce the inhibitor of JEV RNA helicase. In this study, an extracellular protein which has inhibition activity on ATPase activity of JEV RNA helicase was purified from supernatant of Streptomyces chartreusis culture by ammonium sulfate pre-cipitation and size exclusion chromatography. SDS-PAGE analysis showed a single band with aproximate molecular mass of 11,4 kDa, suggesting that the inhibitor was successfully purified into a single protein.
Enzymatic Hydrolysis of Mannan from Konjac (Amorphophallus sp.) Using Mannanase from Streptomyces lipmanii to Produce Manno-oligosaccharides Sasongko, Ashadi; Yopi,; Rahmani, Nanik; Lisdiyanti, Puspita; Saepudin, Endang
Makara Journal of Science Vol. 19, No. 3
Publisher : UI Scholars Hub

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Abstract

Mannan is an abundant polysaccharide that can be found in konjac (Amorphophallus sp.). Mannan can be enzymatically hydrolyzed using mannanase to produce manno-oligosaccharides which can be used as a prebiotic. The aims of this research are to determine the production time of mannanase from Streptomyces lipmanii, perform enzyme characterization, optimize the hydrolysis time, and characterize the hydrolysis product. A qualitative assay using the indicator Congo red showed that S. lipmanii generated a clear zone, indicating that S. lipmanii produced mannanase in konjac medium and possessed mannanolytic activity. Enzyme activity was determined through reducing sugar measurement using the dinitrosalycylic acid method, and optimum enzyme production was achieved at the second day of culture. Characterization of the enzyme showed that hydrolysis was optimum at pH 7 and at a temperature of 50 oC. The reducing sugar content was increased by an increasing the hydrolysis time, and reached an optimum time at 2 h. The degree of polymerization value of three was achieved after 2 h hydrolysis of mannan from konjac, indicating the formation of oligosaccharides. Analysis by thin layer chromatography using butanol, acetic acid, and water in a ratio of 2:1:1 as eluent showed the presence of compounds with a retention time between those of mannose and mannotetrose. Confirmation was also performed by HPLC, based on the retention time.
Beta-Glucosidase 1 (bgl1) Gene Analysis in Mutant and Wild-type of Penicillium sp. ID10-T065 Syafriana, Vilya; Nuswantara, Sukma; Mangunwardoyo, Wibowo; Lisdiyanti, Puspita
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 1 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n1.p1-8

Abstract

In the previous study, Penicillium sp. ID10-T065 has been mutated using ultraviolet (UV), ethyl methyl sulfonate (EMS) and the combination of UV-EMS to increase Beta-glucosidase (bgl) activity. There were three mutants selected, UV13 (UV mutant), EM31 (EMS mutant), and UM23 (UV-EMS mutant). This study examined the mutations in the bgl gene encoding (bgl1) as well as sequence differences between mutants and wild-type of Penicillium sp. ID10-T065. The gene analysis was performed by PCR amplification and sequencing of the bgl1 gene. The results of bgl1 gene sequences (600 bp) from mutants were aligned with the wild-type, it was discovered that there were base alterations from position 2025 to 2050. Mutant UV13 showed the highest base alterations (7 bases) which occurred at position 2027 (T?C); 2036 (T?G); 2040 (T?G); 2047 (G?C); and 2048-2050 (TTG?GGA). Mutant EM31 showed alterations in five bases at positions 2034 (G?A), 2036 (T?G), 2037 (G?C), 2044 (G?C), and 2047 (G?T). Mutant UM23 showed two base alterations at position 2025 (T?A) and 2037 (G?C). UV irradiation and EMS mutation resulted in transition and transversion DNA, whereas the combination of UV-EMS mutation resulted in transversion mutations. Base alterations in UV13 and EM31 mutants, causing missense and silent mutation, while in UM23 mutant only silent mutations occur. The bgl1 gene analysis showed that mutation using UV light was more effective than using EMS or a combination of UV-EMS in Penicillium sp. ID10-T065.
The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM) Paramita, Ni Luh Putu Vidya; Atikana, Akhirta; Sukmarini, Linda; Untari, Febriana; Prayoga, Muhammad Bagas; Maulidia, Faiqoh Nur; Ratnakomala, Shanti; Lisdiyanti, Puspita; Nuryastuti, Titik; Hertiani, Triana
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 3 (2024): December 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.882

Abstract

Nontuberculous mycobacteria (NTM) are environmental microorganisms, also known as opportunistic pathogens, found in patients with pulmonary tuberculosis. The emergence of antibiotic resistance is increased by prolonged antibiotic treatment for NTM infections. Therefore, alternative sources of new antibiotics are essential for the treatment of NTM infections. A marine actinobacterium, Streptomyces sp. BTA 1-131, isolated from a marine sponge, Melophlus sarasinorum, has been reported as a potential source of antibacterials and anticancer agents. The present study aimed to investigate the potential of Streptomyces sp. BTA 1-131 against two NTMs: Mycobacterium smegmatis and Mycobacterium fortuitum. Streptomyces sp. BTA 1-131 was fermented in three cultivation media (SYP, ISP2, and YS), and the secondary metabolites were extracted using methanol. The bioactivity screening showed inhibition of all methanolic extracts against the growth of M. smegmatis and M. fortuitum. The methanolic extract, which could inhibit both mycobacteria, was a crude extract derived from SYP liquid medium fermentation. The isolated compounds in this study were preliminarily identified using thin-layer chromatography (TLC). The TLC results showed different potential compounds in the crude extracts of Streptomyces sp. BTA 1-131 also highlighted the impact of the fermentation medium on the production of metabolites from Streptomyces. This study also added knowledge about the importance of the Indonesian marine actinobacterium Streptomyces sp. BTA 1-131 as a promising producer of anti-NTM compounds.
Co-Authors - Yopi Abd. Rasyid Syamsuri ADE ANDRIANI Adi Santoso Agustinus Robert Uria Ahmad Thontowi Akhmad Rivai Andi Utama ANDI UTAMA ANDO KATSUHIKO Apriliana, Pamella Ariani Hatmanti Ario Betha Juanssilfero Ashadi Sasongko Atikana, Akhirta Caniago, Asnany Caniago, Asnany Desriani Desriani Desriani Desriani Dewi Seswita Zilda dinihari indah kusumawati Dwi Wulandari Elvi Yetti Endang Saepudin, Endang Endang Sukara Erdiansyah Rezamela, Erdiansyah Evi Triana Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi GINA KARTINA Hastuti, Ratih Dwi Herman Suryadi JAKA WIDADA KATSUHIKO ANDO Krause, Janina Kusharyoto, Wien Kusharyoto, Wien Kusumawardhani, Dinihari Indah Kusumawati, Dinihari Indah Kusumawati, Dinihari Indah Lina Elfita Lina Elfita LINDA SUKMARINI M. Iqbal Prawira-Atmaja, M. Iqbal Mangunwidjaja, Wibowo MARIA BINTANG Maulidia, Faiqoh Nur Meliah, Siti Meliah, Siti Mia Kusmiati Miranti Nurindah Sari, Miranti Nurindah MISA OTOGURO Mochammad Imron Awalludin Mulyorini Rahayuningsih Nadhifah, Hana NANIK RAHMANI Nanik Rahmani Neneng Hasanah Neng Herawati, Neng Ni Luh Putu Vidya Paramita NITA ROSALINDA PRAYITNO Nurul Fitri Sari Nuryana, Isa Prasetyoputri, Anggia Pratama, Rendy Eka Prayoga, Muhammad Bagas Putra, Filemon Jalu Nusantara Putra, Masteria Yunovilsa Putri, Ade Lia Putri, Ade Lia Restu Wulansari, Restu Riyona Desvy Pratiwi, Riyona Desvy ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN Seprianto, Seprianto SHANTI RATNAKOMALA Shanti Ratnakomala Shanti Ratnakomala Subagus Wahyuono Suharsono Suharsono Sukma Nuswantara Susanti, Agustina Eko Syafriana, Vilya Tisnadjaja, Djadjat Tisnadjaja, Djadjat Titik Nuryastuti TOMOHIKO TAMURA Toto Iswanto TRI RATNA SULISTIYANI TRI RATNA SULISTIYANI Triana Hertiani Trisanti Anindyawati, Trisanti Tutik Murniasih Ukhradia Maharaniq Safira Untari, Febriana WIBOWO MANGUNWARDOYO Yana Rubiyana, Yana Yantyati Widyastuti Yopi Y YOPI YOPI Yopi Yopi Yopi, YULIN LESTARI Yusro Nuri Fawzya