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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Jurnal Teknologi Pangan dan Gizi Jurnal Penelitian Pascapanen Pertanian Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Indonesian Journal of Biotechnology Animal Production : Indonesian Journal of Animal Production Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Livestock and Animal Research Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Denny Widaya Lukman
Divisi Kesehatan Masyarakat Veteriner, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Journal : Forum Pasca Sarjana

 1 
Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy he Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Rochman Naim; Ewald Usleber
Forum Pasca Sarjana Vol. 32 No. 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy herd and human health.  The aim of this study is to detect MAP in the growing up milk formula.  Fifty samples from five established distributors were taken in Bogor.  Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer.  Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57.  Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia.   Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR
Co-Authors Abdul Zahid Abdulwahed Ahmed Hassan Aditya Primawidyawan Afiff , Usamah Agus Wijaya Agustin Indrawati Ahmad Nurhakim Aminudin, Muhammad Rizki Andrijanto Hauferson Angi Anindya Kurniawati Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Asah Hilaliah Bambang Pontjo Priosoeryanto Bambang Sumiarto Chaerul Basri Chandra Utami Wirawati Debby Fadhilah Pazra Devi Yanti Sari Edi Sukmawinata Eko Agus Srihanto Ekowati Handharyani Engki Zelpina Erfiandini Eka Puspita Etih Sudarnika Eulis Tanti Marlina Ewald Usleber Fachriyan Hasmi Pasaribu Hadri Latif Hasudungan A Sidabalok Hasudungan A Sidabalok, Hasudungan A Heri Yulianto Herwin Pisestyani I Gusti Made Krisna Erawan I Wayan Suardana I Wayan Teguh Wawan I wayan Teguh Wibawan IETJE WIENTARSIH Indra Permana, Indra Inriyanti Assa Irena Ivania Kallau, Novalino Harold Geoffrey Kamil Riski Sidik koekoeh santoso Kumala Andri Asari Loisa Loisa Loisa, Loisa Manuel da Costa Maya Shofa Milawarni Milawarni Minarwati Sudarwanto Mirnawati B Sudarwanto Mirnawati Bachrum Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Naoaki Misawa Nararya Adinata Nugraha, Arifin Budiman Pierre Dorny Platika Widiyani Putra Wijaya, Erlangga Putut Isworo Arimurti Rahmat Setya Adji Rakhmi Ros Sari Ramadhani, Nada Nursaffana Razali Razali Rinaldi Ghurafa Rismayani Saridewi Rismayani Saridewi, Rismayani Risqika Akla Velayati Rochman Naim Ronald Tarigan Roostita L Balia Rotinsulu, Dordia Anindita Saukhan, Falih Prenata Sekarsana, Disty Ayu Siti Gusti Ningrum sri murtini . Sudarwanto, Mirnawati Bachrum Sukmawinata, Eddy Supriyono Supriyono Surachmi Setiyaningsih Surachmi Setyaningsih Surachmi Setyaninigsih Tangkonda, Elisabet Taufiqqurrahman, Mas Trioso Purnawarman Umi Cahyaningsih Widagdo Sri Nugroho Woro Wulandari Kalanjati Wyanda Arnafia Yusuf Ridwan