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ANTIBACTERIAL ACTIVITY OF PAPUYU FISH SCALE CHITOSAN (Anabas testudineus) AGAINST Staphylococcus aureus Aila At-Thoyyar; Muhammad Yanuar Ichrom Nahzi; Dewi Puspitasari; Didit Aspriyanto; Juliyatin Putri Utami
Dentino: Jurnal Kedokteran Gigi Vol 9, No 2 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i2.20399

Abstract

Backgrounds: Pulp disease due to caries can be treated through endodontic treatment, which aims to eliminate bacteria in the root canal so microorganisms cannot multiply. Staphylococcus aureus is a resistant microorganism causing root canal treatment failure. Chlorhexidine as a gold standard cannot remove biofilm and other organic debris. Utilization of nature, chitosan of papuyu fish scales (Anabas testudineus) has antibacterial properties that can be an alternative root canal irrigation material. Purpose: Analyze the antibacterial activity of chitosan of papuyu fish scales against Staphylococcus aureus based on the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Methods: This research is a true experimental design with posttest only with control group design. The research groups were chitosan of Papuyu fish scales with concentrations of 25%, 50%, 75%, 100%, chlorhexidine gluconate 2% (positive control), and aquadest (negative control) with 6 repetitions. Results: The MIC value in the One-Way ANOVA test showed a significant difference (p<0.05). Data analysis followed by Post Hoc Games-Howell test showed all treatment groups had significant differences between each other (p<0.05). The MBC value in the Kruskal Wallis test shows that there is a significant difference (p<0.05). Data analysis followed by MannWhitney test showed significant differences between each other (p<0.05) except for the group of papuyu fish scale chitosan 25% concentration against papuyu fish scale chitosan 50% concentration, papuyu fish scale chitosan 100% concentration against chlorhexidine gluconate 2% (p>0.05). Conclusion: There is a difference in the antibacterial activity of chitosan of Papuyu fish scales concentrations of 25%, 50%, 75%, 100%, chlorhexidine gluconate 2% (positive control) and aquadest (negative control) against Staphylococcus aureus bacteria.
DIFFERENCES IN DMF-T INDEX OF PDAM WATER AND RIVER WATER USERS IN THE BLACKSMITH INDUSTRY AREA Raihatun Nida; Ika Kusuma Wardani; Sherli Diana; Juliyatin Putri Utami; Norlaila Sarifah
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22198

Abstract

Background: Caries is a disease of the oral cavity that affects the hard tissues of the teeth, enamel, dentin, and cementum. Caries is a multifactorial disease or is caused by many factors. One factor that influences the incidence of caries is the water used for daily purposes. Water containing high concentrations of metals can affect the incidence of caries, one of which is iron. Ferrum or iron contained in water can cause caries by increasing H + ions and then continuing to decrease pH, resulting in a demineralization or dissolution process of hydroxyapatite. Caries occurrence can be measured using the DMF-T index. Purpose: This study aims to determine the difference in the DMF-T index of students using PDAM water and river water in the iron industry area of Sungai Pinang Village. Methods: The method used was analytic observational with a cross-sectional design. Respondents in this study were 36 for each group, with a total of 72 respondents. Results: The results of the unpaired T-Test test showed a significance value of 0.00 <0.05, so HO was rejected. Conclusion: There is a significant difference in the DMF-T index of river water PDAM water users in the blacksmith industrial area of Sungai Pinang Village.
TOXICITY TEST OF Eusideroxylon zwageri BARK EXTRACT BASED ON LIVER HISTOPATHOLOGY HYDROPIC DEGENERATION AND NECROSIS Dhiya Salma Azminida; I Wayan Arya Krishnawan Firdaus; Juliyatin Putri Utami; Beta Widya Oktiani; Erida Wydiamala
Dentino: Jurnal Kedokteran Gigi Vol 9, No 2 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i2.20400

Abstract

Background: Ironwood bark extract contains antioxidant properties such as flavonoids, phenolics, and proanthocyanidins, which can stabilize ROS in the body and help accelerate wound healing. The liver plays a role in nutrient metabolism, detoxification, and ROS production. The lack of antioxidants to neutralize excess ROS makes the liver vulnerable to damage. Purpose: This study aimed to determine that there was no toxic effect of giving ironwood bark extract (Eusideroxylon zwageri) doses of 1.250 mg/kg, 2.750 mg/kg, and 4.750 mg/kg on the liver of Wistar rats based on histopathological features of hydropic degeneration and necrosis. Methods: Pure laboratory experimental quantitative (true experimental) with a post-test-only research design with control group design and One Way Anova statistical test. Treatment was given to 4 groups, namely group (K) was given aquadest control, group (T1) was given ironwood bark extract at a dose of 1.250 mg/kgBW, group (T2) was given ironwood bark extract at a dose of 2.750 mg/kgBW, and group (T3) given ironwood bark extract at a dose of 4.750 mg/kgBW. Results: The results showed no significant difference between the four treatment groups based on the average percentage of hydropic degeneration and necrosis. Conclusion: There was no toxic effect of giving ironwood bark extract doses of 1.250 mg/kgBW, 2.750 mg/kgBW, and 4.750 mg/kgBW on the liver of Wistar rats based on histopathological appearance of hydropic degeneration and necrosis for 14 days.
Color Stability of Nanohybrid Resin Composites Immersed in Kalangkala (Litsea angulata) Leaf Extract and Non-Alcoholic Mouthwash Wardhana, Agung Satria; Ruzhanah, Huda; Dewi, Renie Kumala; Erlita, Isyana; Hamdani, Riky; Utami, Juliyatin Putri
Denta Journal Kedokteran Gigi Vol 20 No 2 (2026): August
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v20i2.5

Abstract

Background: Dental caries is commonly treated using tooth-colored restorative materials such as resin composites. However, composite resin restoration may discolor due to long term exposure of mouthwash. Newest herbal research introduces an extract of Kalangkala (Litsea angulata) leaves as a potential mouthwash derived from Kalimantan’s local plant. Kalangkala leaf extract has demonstrated antibacterial activity against oral bacteri. Kalangkala leaf also has a potential in reducing side effects from synthetic ingredients in conventional mouthwash. Objective: To analyze color stability of nanohybrid resin composite after immersion in 3% Kalangkala extract and non-alcoholic mouthwash. Materials and Methods: This experimental study used a post-test and control group design using 30 samples of resin composite using ISO 4049(2000) standardization. Group 1 was immersed in kalangkala extract, group 2 immersed in non-alcohol mouthwash, and group 3 in distilled water for 31 hours. The picture was taken with a high-resolution digital camera and lightning device for brightness standardization. Color changes were measured using MATLAB software with CIELAB color system. Data were analyzed statistically using ANOVA. Results: Although the Kalangkala extract group showed the highest mean color change, no statistically significant difference were found among the three immersion solutions (p=0.971). Conclusion: The Immersion in kalangkala leaf extract did not significantly affect the color change of nanohybrid resin composite.
Co-Authors Adhiya, Geyanina Melda Adhytya Suryo Kelana Agung Satria Wardhana Aila At-Thoyyar Ainna Dewi Iriani Amalia, Aysca Fakhira Amy Nindia Carabelly Andi Muthia Azizah Tenri Bali Anisa Novia Farrasti Annisa Noviany Aprilyani, Nur Arifin, Rahmad Aspriyanto, Didit Baitullah, Muhammad Akbar Bambang Setiawan Bayu Indra Sukmana Beta Widya Oktiani Budiarman, Andi Azizah Maulidia Dewi Indah Noviana Pratiwi Dewi Indah Noviana Pratiwi, Dewi Indah Noviana Dewi Nurdiana Dewi Purboningsih Dewi Puspitasari Dewi, Renie Kumala Dhiya Salma Azminida Diana Lyrawati Diana Wibowo Erida Wydiamala Erika Norfitriyah Eriwati, Yosi Kusuma Erni Marlina Febriansyah, Muhammad Firda Damayanti Firdaus, I Wayan Arya Krishnawan Fransiska Uli Arta Panjaitan Gita Puspa Ningrum Gusti Erysa Nur Tsaniya Hadi Waskito Haluanry Doane Santoso Hamdani, Riky Hatta, Isnur I Wayan Arya Krishnawan Firdaus Ichrom Nahzi, Muhammad Yanuar Idhafi, Nasrul Ika Kustiyah Oktaviyanti Ika Kusuma Wardani Jatmiko, Habibi Naufal Kurnianingsih, Nia Lena Rosida Lena Rosida Maghfirah, Yolanda Sajjida Maharani Laillyza Apriasari Maharani Lailyza Apriasari Mahmud Muhlisin Maria Tanumihardja, Maria Maria Ulfah Maulida, Novi Dwi Melisa Budipramana, Melisa Milka Widya Sari Mira Hadistiana Monita Valentine Silalahi Muhammad Adeya Herdira Putra Muhammad Akbar Baitullah Muhammad Hasanu Reksi Muhammad Reza Faisal, Muhammad Reza Muhammad Rizal Bima Saputra Nahzi, Yanuar Ichrom Namira Fathya Salsabila Nanda Putri Arini Nia Kurnianingsih Nisa Fachrizha Munier Nor Rahman Sugiarto Norfitriyah, Erika Novi Dwi Maulida Novia Damayanti Nurdiana Nurdiana Nurrahman, Tri Panghiyangani, Roselina Pramitha, Selviana Rizky Priyawan Rachmadi Rachmayani, Nevrila Dewi Rahmad Arifin Raihatun Nida Ramadhaniyah, Nur Rizki Aprian Roselina Panghiyangani Roselina Panghiyangani Ruzhanah, Huda Safa Muzdalifah Sandra Bhakti Mafriana Sari, Galuh Dwinta Sari, Milka Widya Sarifah, Norlaila Sherli Diana Silalahi, Monita Valentine Siti Wasilah Tri Putri, Deby Kania Wardhana, Agung Satria Yunike Christanti Yusrinie Wasiaturrahmah