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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology ZOO INDONESIA ARSHI Veterinary Letters Dinamika Informatika: Jurnal Ilmiah Teknologi Informasi PARADIGMA Borneo Journal of Science and Mathematics Education Borneo Journal of Primary Educaton Al-Muraqabah: Journal of Management and Sharia Business Jurnal Kedokteran Hewan Jurnal Kajian Ekonomi dan Manajemen Indonesia Policy Brief Pertanian, Kelautan, dan Biosains Tropika Al-Insyiroh: Jurnal Studi Keislaman Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Tarbiyah Jamiat Kheir Research Economics Management and Business Jurnal Bioteknologi & Biosains Indonesia
MUHAMMAD AGIL
Bagian Reproduksi Dan Kebidanan Departemen Klinik Reproduksi Dan Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor
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Journal : Jurnal Veteriner

 1 
Profil Gonad Kodok Lembu Betina yang Diberi Human Chorionic Gonadotropin dan Ekstrak Hipofisis Kodok Lokal I Ketut Mudite Adnyane; Sevy Tiara Ilham; Muhammad Agil
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bulllfrog (Rana catesbeiana) are naturally reach sexual maturity at the age of 3 years aftermetamorphosis. This research aims to study the gonad growth of female bullfrog. given human chorionicgonadotropin (hCG) and hipofise extract of local frog in order to accelerate the maturity. There were 40female bullfrog used in this study and divided into four treatment groups and one control. The treatmentswere injections of 150 IU hCG, 200 IU hCG, 8 hipofise, 16 extract of hipofise and controls. The injectionswere conducted once a month for four months. Every month after the first injection, two of bullfrogs fromeach group were measured their body and gonad weight, in addition, the stage of ovary was also observedeither in macroscopically and microscopically. The results showed that hCG treatment group gave betterresults compared with the treatment of hipofise extract of local frog. The injection of 200 IU hCG gave thebest result of gonad maturity index (IKG) 4.9%, compared with other treatment groups. Giving a dose of200 IU hCG and 16 hipofise exctract of local frog can be used to accelerate the maturity of female bullfrog
Histologi dan Histomorfometri Testis dan Epididimis Muncak (Muntiacus muntjak muntjak) pada Periode Ranggah Keras (HISTOLOGY AND HISTOMORPHOMETRY OF THE TESTIS AND EPIDIDYMIS OF MUNTJAC (MUNTIACUS MUNTJAK MUNTJAK) DURING HARD ANTLER PERIOD) Sri Wahyuni; Srihadi Agungpriyono; Muhammad Agil; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to describe the histology and histomorphometry of testis and epididymisof muntjac (Muntiacus muntjak muntjak) during hard antler period. The tissues of the testis and epididymisof an adult male muntjac were processed for histological examination and stained with haematoxylineosine(HE). The parenchyma of muntjac’s testis during hard antler period showed tubuli seminiferi waslined with germinal epithelium: spermatogonia, spermatocyte, spermatid that differentiated intospermatozoa. Sertoli cells were found among the germinal cells. In addition, Leydig cells were foundaround the blood vessel of interstitial tissue along with macrophages. Diameter of the seminiferous tubuleand epithelial thickness were 176,60±7,06 ?m and 50,27±3,62 ?m respectively. The epididymal duct wassubdivided into three segments: caput, corpus and cauda. They were lined predominantly withpseudostratified columnar epithelium which was varied in its thickness. The largest diameter of epididymalduct was found in cauda region (324,26±25,79 ?m), while caput epididymidis had the thickest of epithelialcell (62,21±4,21 ?m) and tended to ce thinner in corpus (49,53±3,01 ?m) and cauda epididymidis(16,30±2,27?m). The density of spermatozoa was observed the most in the lumen of cauda region comparedto caput and corpus epididymidis. In conclusion, the structure of histology and histomorphometry of theseminiferous tubule of testis and epididymal duct of muntjac were similar with small ruminants andother Cervidae during hard antler period.
Deteksi Umur Pubertas Muncak (Muntiacus muntjak muntjak) Betina Berdasarkan Analisis Metabolit Estrogen dan Progesteron pada Feses (THE AGE OF PUBERTY DETECTION IN FEMALE BARKING DEER (MUNTIACUS MUNTJAC MUNTJAC) BASED ON FAECAL ESTROGEN AND PROGESTERONE A Asri Pudjirahaju; Iman Supriatna; Srihadi Agungpriyono; Muhammad Agil
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Knowledge and information about the age of puberty in muntjac (Muntiacus muntjac muntjac) isindispensable to the interests of females breeding in conservation efforts. The aims of this study were todetermine the age of puberty and age at first mated females muntjac kept in captivity through the analysisof estrogen and progesterone metabolites in feces. This study used 155 fecal samples that were collectedfrom three female muntjacs. Sample collection was began when muntjac aged three months, four monthsand six months. Total of 10-20 g fecal samples were collected every 2-4 days. Analysis of steroid hormonemetabolites was performed by using enzyme immunoassay (EIA) method with specific antibodies.Determination of the age of puberty was based on the appearance of the first time estrus and ovulation,which was indicated by the appearance of the highest estrogens secretion, on hormone metabolites profile.Hormone metabolites data then were tabulated in the average and standard deviations were presentedwith graphs and analyzed descriptively. The results showed that the age of puberty detection based onanalysis of the estrogens and progesterone metabolite in the feces can be applied in muntjac. Muntjacfemales kept in captivity flats reached puberty at age 5±1 month or 4-6 months range. It is recommendedthe first mated in the muntjac is at least after the female experienced two period of oestrous or has reachedat age of six months.
Validasi Kit Enzyme-Linked Immunosorbent Assay Komersial untuk Analisis Hormon Estradiol dan Progesteron Darah Kambing Kacang (VALIDATION OF COMMERCIAL ENZYME-LINKED IMMUNOSORBENT ASSAYKIT FORANALYSIS OF ESTRADIOLANDPROGESTERONE HORMONE IN BLOOD OF KACANG Dedi Rahmat Setiadi; Iman Supriatna; Muhammad Agil
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to evaluate the capability of two human commercial enzyme-linkedimmunosorbent assay (ELISA) kits(DRG International Inc.,Germany and GBC Taiwan) for measuringestradiol (E2) and progesterone (P4) in plasma of kacang goat. Three healthy and non pregnant femalekacang goats aged 2-3 years with regular estrous cycles were used in this study. Blood samples werecollected from the jugular vein using a 21G venoject every two days and it was intensified every day duringthe period before heat. Collected plasma were stored at -20ºC until the analysis. Capability validationwas conducted by measuring accuracy (parallelism test), sensitivity and precision.Parallelism test usingDRG commercial kit showed that sample curvewas parallel with standard curve of E2 and P4. In contrastit was not parallel with standard curve of GBC commercial kit. Sensitivity was measured from the lowesthormones concentration of E2 and P4 at 90% binding that were 25 pg/ml and 0.14ng/mL in DRG kit, whilein GBC kit were 5 pg/mL of E2 and 0.2 ng/mL of P4, respectively. Coefisien of variation of intra- andinterassay for both ELISA commercial kits were less than10%. It can be concluded that DRG commercialELISA kit E2 and P4 can be used to analyse female kacang goat blood plasma, while GBC commercialELISA kit E2 and P4 are not recommended.
Co-Authors A Amrozi Abdul Samik Adji, Vita Adonia Afiff , Usamah Ahmada, Milla Alwi, Alwi Shobri Amala, Karisma Wahyu Amarudin, Amin Awal Amrozi Amrozi, A Andre Pasetha Anriansyah Renggaman Antje Engelhardt Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Asmawan, Yudhi Ferdi Andri Asri Pudjirahaju Bakti Tamba Bambang Purwantara Berlin Pandapotan Pardede Borman, Mohammad Riski Cece Sumantri Dandan Haryono Dea Indriani Astuti DEDI CANDRA Dedi Rahmat Setiadi Desy Desy, Desy Dilla frastantie Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Entang Iskandar Fathur Rahman Fitriani, Lia Gholib Gholib Gholib Gholib, Gholib Gono Semiadi GONO SEMIADI Gusman, Khalis Talitha Hamidudin, Ahmad Yahya Hamny Sofyan Harianto Budi Rahardjo Heistermann, Michael Hermansyah, Endang HUSNUL KHOTIMAH I Ketut Karya Wisana Idawati Nasution Iis Afriantini Iman Supriatna Intam Kurnia Islachiyana, Rifda Jansen Manansang Jansen Manansang Juli Melia Juli Melia Juli Melia Ketut Adnyane Mudite koekoeh santoso La Ode Muhammad Aswad Salam Lidya Elizabeth M. Manik Lisa Michelle Danish M Agus Setiadi Mahmudi Mahmudi Marniati Kadir Michael Heistermann Minarti, Melisa Muchidin Noordin Muhammad Fathoni, Muhammad Muhammad Jalaluddin Muhammad Subhan Muhammad Syawal Muhammad Syawal Muslim Akmal Mustainah Mappatoba Nanik Hidayatik Ni Wayan Kurniani Karja Nisa, Hairun Nisa Pardede, Berlin Pandapotan Putra, Saiful Aminudin Al Kusuma R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Ridzky Pratama Sartika Lina Mulani Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Sevy Tiara Ilham Sholikhah, Noviana Nur Sinta Maryam, Sinta Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Surya Agus Prihatno Sutrisnak Sutrisnak Taufiq P. Nugraha Teguh Sumarsono Tuty L Yusuf Tuty L Yusuf Tuty L Yusuf Tuty L. Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardy Yusuf Vincentia Trisna Yoelinda Wahono Esthi Prasetyaningtyas Wahyuni, Sri Yohana Tri Hastuti Yohana Tri Hastuti Yosua Kristian Adi Zunaidi, Arif