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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Teknologi Industri Pertanian Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Akuatika Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrointek Jurnal Hortikultura Current Biochemistry BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Indonesian Journal of Biotechnology Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Sumberdaya Hayati Journal of Tropical Biodiversity and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Purifikasi Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Menara Perkebunan Jurnal Mangifera Edu Journal of Microbial Systematics and Biotechnology Konservasi Hayati Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) MADURANCH: JURNAL ILMU PETERNAKAN Makara Journal of Science International Journal of Oil Palm

NISA RACHMANIA MUBARIK

Bogor Agricultural University

Author-ID : 322460

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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KONSISTENSI PRODUKSI NATA DALAM MEDIA FERMENTASI YANG MENGANDUNG HIDROLISAT UBI KAYU Lutfansyah Muchtar, Nisa Rachmania Mubarik, dan Antonius Suwanto
Jurnal Teknologi Industri Pertanian Vol. 27 No. 2 (2017): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Nata de coco is known as a gelatinous substance, white to creamy-yellow, firm, glossy and not sticky, produced by some bacteria, which forms on the surface of coconut water medium during fermentation. Acetobacter xylinum (current name: Gluconacetobacter xylinus) is the common bacteria which synthesized nata de coco. Besides coconut water, there are some alternatives for making nata, such as cassava. Gluconacetobacter sp. was employed in this study to produce nata de coco from cassava hydrolysate medium (U) and compared it qualitatively and quantitatively to coconut water medium (K). In U medium, glucose was the main product derived from hydrolysis of cassava. Fermentation was conducted three times with each former fermentations became sources of inocula for the next fermentations, respectively. All data were analysed with IBM SPSS Statistics 23. Fermentation product of nata from U medium were relatively stable in thickness, wet weight, dry weight, yield, water content, and log from total bacteria (quantitative) and in colour, texture, transparency, and surface of nata (qualitative). Glucose was rapidly consumed by bacteria then other sugar (fructose and sucrose). Glucose consumption and nata yield was affected by the number of bacteria. However, nata yield was not significantly influenced by glucose consumption. Nata yield was possibly influenced by other factors such as nutrient content in U medium. Growth dynamics of Gluconacetobacter sp. was measured in log cfu mL-1 on Hassid-Barker Agar (HBA) medium and relative number of 16S rRNA genes. Bacterial growth during fermentation in U medium were relatively more stable than fermentation on K medium although the yield in K medium gradually increased and were higher than U medium. Keywords: cassava hydrolysate, Gluconacetobacter sp., nata, stable fermentation

PRODUKSI LIPASE DARI ISOLAT KAPANG HASIL MUTASI UNTUK TRANSESTERIFIKASI Galih Cendana Nabilasani; Trismilah Siswodarsono; Dadang Suhendar; Nisa Rachmania Mubarik
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Lipase Production by Mutant Fungal Isolates for TransesterificationÂ ABSTRACTLipase is used amongst others in biodiesel production, namely in the transesterification reaction. Kernel B (KB) was a fungus isolated from the waste of palm kernel and seed. The fungus produced lipase that catalysed the transesterification reaction with a lower activity compared to that of AK Amano commercial lipase. The purpose of this study was to obtain mutant fungi with higher transesterification activities than the wild type (KB). The mutation process was carried out using ultraviolet (UV) light, ethyl methane sulfonate (EMS), and N-methyl-Nâ€™-nitro-N-nitrosoguanidine (NMNG) on KB fungus. The mutations using UV light produced 11 isolates, of which isolate m4.1KB1 produced a higher transesterification activity (0.172 UÂ·mg-1) compared to the wild type. Mutant m5.7KB, which was generated from mutant m4.1KB1 treated using EMS, had its transesterification activity decreased to only 0.051 UÂ·mg-1. Mutant m6.0,3KB2, which was resulted through NMNG treatment, experienced an increase in transesterification activity which was 91.2% higher than that of KB.Keywords: ethyl methane sulfonate, lipase, mutant fungi, N-methyl-Nâ€™-nitro-N-nitrosoguanidine, ultravioletÂ ABSTRAKLipase dimanfaatkan salah satunya dalam produksi biodiesel, yaitu dalam reaksi transesterifikasi. Kernel B (KB) merupakan kapang yang diisolasi dari limbah inti dan biji kelapa sawit, yang menghasilkan lipase sebagai katalis dalam reaksi transesterifikasi. Namun aktivitas transesterifikasi yang dihasilkan oleh lipase dari KB lebih rendah dibandingkan dengan lipase komersial AK Amano. Tujuan penelitian ini adalah mendapatkan mutan kapang dengan aktivitas transesterifikasi yang lebih tinggi dibandingkan tipe liarnya (KB). Proses mutasi dilakukan dengan menggunakan sinar ultraviolet (UV), ethyl methane sulfonate (EMS), dan N-methyl-Nâ€™-nitro-N-nitrosoguanidine (NMNG) terhadap kapang KB. Mutasi KB dengan menggunakan sinar UV menghasilkan 11 isolat, dimana isolat dengan kode m4.1KB1 menghasilkan aktivitas transesterifikasi yang lebih tinggi dibandingkan tipe liar, yaitu 0,172 UÂ·mg-1. Mutan m5.7KB, yang dihasilkan dari mutan m4.1KB1 dengan perlakuan EMS, mengalami penurunan aktivitas transesterifikasi hingga hanya sebesar 0,051 UÂ·mg-1. Mutan m6.0,3KB2 hasil perlakuan NMNG mengalami peningkatan aktivitas transesterifikasi sebesar 91,2% lebih tinggi dari KB.Kata Kunci: ethyl methane sulfonate, kapang mutan, lipase, N-methyl-Nâ€™-nitro-N-nitrosoguanidine, ultraviolet

AKTIVITAS ANTIOKSIDAN EKSTRAK KAPANG ENDOFIT Cb.Gm.B3 ASAL RANTING KAYU MANIS (Cinnamomum burmanni) Fauzy Rachman; Nisa Rachmania Mubarik; Partomuan Simanjuntak
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Antioxidant Activity of Endophytic Fungi Cb.Gm.B3 Extract from CinnamonÂ (Cinnamomum burmanni) TwigsABSTRACTThere are many degenerative diseases that are caused by a free radical effect. Cinnamon (Cinnamomum burmanni) contains cinnamaldehyde compounds that have activity as a powerful antioxidant and fight free radicals. Endophytic fungi can be found in cinnamon plants living symbiotically. Endophytic fungi produce a variety of bioactive metabolites including antioxidants. This research was conducted to isolate endophytic fungi from C. burmanni plant which is active as antioxidant. Endophytic fungi isolation was carried out using surface sterilization method and cultivated in PDA media. Antioxidant activity test was performed using free radical 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. Selected isolates were then identified molecularly to determine their species. A total of nine fungi were isolated from cinnamon twigs. The result showed that the highest antioxidant activity was obtained from Cb.Gm.B3 with IC50 of 13.219 Â± 0.755 Âµg/mL. The selected isolate Cb.Gm.B3 taxonomically has a high similarity with Neofusicoccum parvum isolate PEL23 (Accession no: KY053054.1).Keywords: antioxidant, Cinnamomum burmanni, 2.2-diphenyl-1-picrylhydrazyl, endophytic fungi, Neofusicoccum parvumÂ ABSTRAKKayu manis (Cinnamomum burmanni) mengandung senyawa sinamaldehid yang memiliki aktivitas sebagai antioksidan kuat dan dapat menangkal radikal bebas. Dalam tanaman kayu manis terdapat kapang endofit yang hidup bersimbiosis. Kapang endofit dapat menghasilkan berbagai senyawa metabolit bioaktif termasuk antioksidan. Penelitian ini dilakukan untuk mengisolasi kapang endofit dari tanaman C. burmanni yang aktif sebagai antioksidan. Isolasi kapang endofit dilakukan menggunakan metode sterilisasi permukaan dan ditanam pada media PDA. Pengujian aktivitas antioksidan dilakukan menggunakan metode peredaman radikal bebas dengan reagen 2.2-difenil-1-pikrilhidrazil (DPPH). Isolat terpilih diidentifikasi secara molekuler untuk menentukan spesiesnya. Sebanyak 9 isolat kapang berhasil diisolasi dari jaringan ranting tanaman kayu manis. Aktivitas antioksidan tertinggi (IC50) didapatkan dari isolat Cb.Gm.B3 sebesar 13,219 Â± 0,755 Âµg/mL. Isolat terpilih Cb.Gm.B3 secara taksonomi memiliki tingkat kemiripan yang tinggi dengan Neofusicoccum parvum isolat PEL23 (No. aksesi: KY053054.1).Kata Kunci: antioksidan, Cinnamomum burmanni, 2.2-difenil-1-pikrilhidrazil, kapang endofit, Neofusicoccum parvum

ENERGI LISTRIK DARI SEDIMEN LAUT TELUK JAKARTA MELALUI TEKNOLOGI MICROBIAL FUEL CELL Bambang Riyanto; Nisa Rachmania Mubarik; Fitriani Idham
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14 No 1 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Sediment microbial fuel cell (SMFC) is one form of the microbial fuel cell (MFC) that can convert organic complex material in the sediment to generate electrons. This research was conducted to determine the characteristicsof marine sediments from the Bay of Jakarta, to know the amount of electric current can be generated through SMFC, and to identify bacteria at the anode SMFC. This research was conducted in several stages which include the characterization of marine sediment samples, making SMFC circuit, electric current measurement, characterization SMFC substrate, and the isolation, characterization, and identi cation of bacteria. The study shows that marine sediments of Jakarta Bay have characteristics which include silty clay loam texture, organic carbon 2.19%, total nitrogen 0.19%, and phosphorus 128 ppm. The electric current generated by the SMFC using  xed value resistors 820 Ω ± 5% reach peak production of electric currents on day 21, that is 139.51 mA/m2 with SMFC substrate having organic carbon 1.88%, total nitrogen 0.15%, and phosphorus 88 ppm. Isolation of bacteria at the anode was found three types of isolates, that suspected are Aeromonas hydrophila, Acinetobacter sp., and Bacillus marinus.Keywords: electrical energy, Jakarta bay, marine sediment, sediment microbial fuel cell

PENAPISAN BAKTERIOSIN DARI BAKTERI ASAM LAKTAT ASAL BEKASAM Desniar .; Iman Rusmana; Antonius Suwanto; Nisa Rachmania Mubarik
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14 No 2 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Bacteriocin is proteinaceous compound that has bactericidal action against other microorganisms. Bacteriocin-producing lactic acid bacteria (LAB) is generally considered safe for human consumption and can be applied in food preservation. One source of indigenous LAB is from Indonesian fermented fish products, bekasam. This study aimed to obtain LAB isolates from bekasam that have high potential as producer of bacteriocin. The steps were screening of bacteriocin compound and protein precipitation using ammonium sulfate with a concentration of 0-10% to 70-80%. Screening of bacteriocin compounds of 25 isolates LAB from bekasam showed that there were 11 isolates (44%) that have the potential as producer of bacteriocin, in which the cell-free supernatant to pH 5 and or pH 6 produce inhibitory zones on the indicator bacteria Escherichia coli, Salmonella typhimurium ATCC 14028, Bacillus cereus, Staphylococcus aureus and Listeria monocytogenes. Then, the precipitation of proteins from the cell-free supernantant was done for the selected four isolates that have the potential as producer of bacteriocin. The supernatant and the precipitate from yield of protein precipitation in the selected four isolates showed that inhibition zone against the indicator bacteria E. coli, S. typhimurium ATCC 14 028, and L. monocytogenes with inhibition zone around 3.0 to 10.0 mm. Inhibition zones in the supernatant and the precipitate were indication that active compound is organic acid and bacteriocin, respectively. The highest inhibition zone of the supernatant and the precipitate of the BP(3) and SK(5) isolates against L. monocytogenes and S. typhimurium, respectively. The highest inhibition zone of the supernatant of the BP(20) and BI(3) isolates against S. typhimurium and S. typhimurium and E. coli, respectively. While the highest inhibition zone of precipitate of the BP(20) and BI(3) isolates were same, that is against E. coli. Each with ammonium sulfate concentrations were different.Key words: Bacteriocin, lactic acid bacteria, bekasam

Soil Chitinolytic Bacteria from Jambi Province to Produce Antifungal of Plant Pathogens Risky Hadi Wibowo; Sipriyadi Sipriyadi; Nisa Rachmania Mubarik; Iman Rusmana
Mangifera Edu Vol 5 No 1 (2020): Jurnal Mangifera Edu
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/mangiferaedu.v5i1.95

Chitinolytic bacteria are bacteria that have chitinolytic activity, which is able to hydrolysis the composition of chitin which composes many fungal cell walls. Chitinolytic bacteria are currently more widely used because of their ability as a biological control agent to the pathogenic fungi especially in horticultural and plantation crops. This research was conducted with the aim of obtaining isolates of chitinolytic bacteria that were able to inhibit the growth of plant pathogenic fungi in Vitro on chitin agar media. Fusarium oxysporum, Sclerotium rolfsii, and Rhizoctonia solanii are used in the inhibition test of chitinolytic bacteria. Bacteria were isolated and screened from the soil of Bukit Dua Belas National Park and Oil Palm Plantations in Jambi using 0.3% chitin agar media. The results showed that two of 10 bacterial isolates were able to produce inhibition zones to the growth of hyphae of pathogenic fungi on potato dextrose agar (PDA) media. TB04-13 isolate was able to produce the largest inhibition in F. oxysporum and R. solanii about 42% and 42.05% respectively, while TB04-15 isolate produced the biggest inhibition in S. Rolfsii ranged to 25.50%. Based on the chitinolytic index (CI) values, isolates TB04-13 and TB04-15 produced CI values of 1.60 and 0.63, respectively. The morphological characteristics and Gram staining of both TB04-13 and TB04-15 chitinolytic isolates are included in rod-shaped and Gram-positive bacteria. Both of these isolates can be used as antifungal-producing candidates for plant pathogenic fungi in Indonesia.

Keragaan Morfo-Fisiologi Phaseolus spp. yang Ditanam Pada Ketinggian Tempat yang Berbeda (Morpho-Physiological Performance Phaseolus spp. on Different Altitudes) Delfi Trisnawati; nFN Triadiati; Nisa Rachmania Mubarik
Jurnal Hortikultura Vol 28, No 1 (2018): Juni 2018
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v28n1.2018.p59-66

Permintaan masyarakat di Indonesia terhadap tanaman legumes meningkat setiap tahunnya, namun produksi legumes di Indonesia belum dapat mencukupi kebutuhan konsumen. Pertumbuhan tanaman legume dipengaruhi oleh faktor lingkungan seperti ketinggian tempat, suhu, intensitas cahaya matahari, dan kelembapan. Tujuan penelitian ini untuk menganalisis dan mengevaluasi keragaan morfo-fisiologi Phaseolus spp. yang ditanam pada dua ketinggian tempat yang berbeda. Empat jenis legumes yang digunakan adalah Jackson Wonder Bean (JWB - Phaseolus lunatus), Christmas Bean (CHB - P. lunatus), Cranberry Bean (CRB - P. vulgaris), dan White Kidney Bean (WKB - P. vulgaris). Empat macam kacang tersebut ditanam pada ketinggian tempat 1.100 m dpl. (lokasi I) dan pada ketinggian 250 m dpl. (lokasi II). Peubah fisiologi dipengaruhi oleh lokasi penelitian, sedangkan peubah morfologi dipengaruhi oleh interaksi antara ketinggian tempat dan jenis kacang. Perbedaan kondisi lingkungan memengaruhi habitus JWB (P. vulgaris). CHB (P. lunatus) menghasilkan jumlah polong tertinggi pada ketinggian tempat 1.100 m dpl., yaitu 25,7 polong diikuti dengan BB 100 biji, yaitu 158,3 g. Implikasi penelitian ini adalah pembudidayaan tanaman legumes atau kacang minor pada dua lokasi yang berbeda berdasarkan ketinggian tempat untuk mendapatkan kondisi lingkungan yang sesuai agar pertumbuhan dan produksinya optimal.KeywordsPhaseolus lunatus; Phaseolus vulgaris; Bintil akar; Konduktansi stomata; Laju fotosintesisAbstractThe demand of legumes in Indonesia is increasing every year. The growth performance of legumes is influenced by environmental factors such as altitude land, temperature, light intensity, and air humidity. The aims of this study were to analyze and evaluate the morpho-physiological performance of legumes (Phaseolus spp.) grown in two different altitudes. Four genotypes of bean used in this study were Jackson Wonder Bean (JWB - P. lunatus), Christmas Bean (CHB - P. lunatus), Cranberry Bean (CRB - P. vulgaris), and White Kidney Bean (WKB - P. vulgaris). The four species of beans were grown at 1,100 m asl. (location I) and 250 m asl. (location II). The physiological parameters were influenced by location, meanwhile the morphological parameter was affected by the interaction between the altitude and type of beans. The differences on environmental condition in both field affected the habits of WKB (P. vulgaris). The CHB (P. lunatus) bean produced the highest number of pods at altitude of 1,100 m asl followed by wet weight of 100 seeds is 25.7 and 158.3. The implication of this research is the cultivation of beans minor at two different locations based on the altitude to get the appropriate environmental conditions to be optimal growth and production.

SENYAWA ANTIMIKROBA YANG DIHASILKAN OLEH BAKTERI ASAM LAKTAT ASAL BEKASAM Desniar -; Iman Rusmana; Antonius Suwanto; Nisa Rachmania Mubarik
Jurnal Akuatika Vol 3, No 2 (2012): Jurnal Akuatika
Publisher : Fakultas Perikanan dan Ilmu Kelautan

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Bakteri asam laktat (BAL) adalah mikroba dominan yang ditemukan dalam fermentasi ikan. Tujuan penelitian ini adalah menentukan perkiraan kuantitatif awal dari substansi antimikroba yang dihasilkan oleh isolat BAL asal bekasam dan mengetahui aktivitas antimikrobnya terhadap lima bakteri patogen. Perkiraan kuantitatif asam laktat dan H2O2, menggunakan metode titrasi. Uji aktivitas antimikroba menggunakan metode difusi sumur agar. Karakterisasi (morfologi, fisologi dan pertumbuhan) dan identifikasi menggunakan API 50 CHL (Bio-Mereux, France). Produksi asam laktat dan H2O2 meningkat dengan waktu inkubasi untuk semua isolat kecuali pada isolat BP(3). Produksi asam laktat tertinggi adalah 21,765 g/L yang dihasilkan oleh isolat SK(5) (48 jam inkubasi). Konsentrasi H2O2 yang dihasilkan oleh semua isolat jauh lebih rendah dibandingkan dengan asam laktat. Konsentrasi H2O2 tertinggi ialah 0,079 g/L pada isolat BI(3) dan BP(20) dalam 72 jam inkubasi. Supernatan bebas sel yang dinetralkan tidak menghambat pertumbuhan bakteri uji, sedangkan yang tidak dinetralkan dapat mengambat bakteri uji yang digunakan dengan zona hambat 9 -15 mm. Zona penghambatan terbesar dihasilkan oleh isolat SK(5) (24 jam inkubasi) terhadap S. aureus. Isolat BI(3), BP(3) dan BP(20) adalah Pediococcus pentosaceus 1 dengan kemiripan sebesar 99,9%. Isolat SK(5) adalah Lactobacillus plantarum 1 dengan kemiripan sebesar 99,9%. Penelitian ini menunjukkan bahwa isolat BAL asal bekasam dapat dijadikan sebagai kandidat biopreservatif pangan terutama untuk pengolahan hasil perikanan. Kata kunci: antimikrobial, asam laktat, bakteri asam laktat, bekasam, dan hidrogen peroksida.

Characterization of Gibberellin Producing Rhizobacteria Isolated from Soil Forest in Banten Hadi Susilo; Nisa Rachmania Mubarik; Triadiati Triadiati
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Gibberellin is plant growth regulator that stimulates cell elongation, seed germination, flowering, and fruit ripening. This study was conducted to isolate, identify, and optimize growth media for gibberellins producing- rhizobacteria isolated from rhizosphere soil of “keruing“ (Dipterocarpus sp.) tree in forest research Carita, Pandeglang, Banten. Eight bacterial isolates were obtained and all produced gibberellin. The BC2 isolate produced the highest of gibberellin (0.897 mg mL-1) and then selected for identification based on physiology, molecular character, and effects of growth media with variation of temperature, pH, and light. The result of physiological test indicated that BC2 isolate does not produce indole, positive on urease and oxidative carbohydrate. The phylogenetic analysis showed that BC2 isolate is belonged to Stenotrophomonas maltophilia with 98% similarity level. The optimation of growth media indicated that the growth of BC2 isolate was optimum at 30°C, pH 7, and dark condition.

Detection and Cloning of a Gene Involved in Zwitermicin A Synthesis from Plant Growth Promoting Rhizobacteria of Bacillus sp CR64 Aris Tri Wahyudi; Rika Indri Astuti; Nisa Rachmania Mubarik; Sarah Asih Faulina
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

Utilization of soil bacteria as biocontrol agent is becoming popular due to its valuable and effective mechanisms to suppress plant pathogenic microbes. We have previously isolated Bacillus sp, designated as Bacillus sp CR64, which exhibited effective plant growth promoting and antifungal activities. In this study, CR64 was examined in inhibiting the growth of Rhizoctonia solani, the causing agent of root rot disease. Partial sequence analysis of 16S rRNA gene revealed that this isolate similar with Bacillus cereus (94%). Furthermore, a gene designated zmaR was detected by means of specific amplification of DNA fragment approximately 950 bp. This fragment was then cloned onto pCRII-TOPO (3.9 kb) and sequenced using DNA sequencer ABI PRISM 310. Sequence analysis revealed that it had highest homology with the ZmaR protein (89% identity; 90% similarity) of B. thuringiensis serovar kurstaki (AAF82729.2). Alignment analysis with other ZmaR sequences from other antibiotic-producing Bacilli exhibited an almost fully conserved region within ZmaR sequences.Key words : PGPR, Bacillus sp CR64, Zwitermicin A, Cloning, Antifungal.

Co-Authors , Triadiati . QATRUNNADA Aas Ratnasari Ahmad Suryadi Ainia Hanifitri Alfan Cahyadi Alfaridza, Annisa Nourma ALINA AKHDIYA Andreas Adhi Satya Andreas Adhi Satya ANGELIA REZTY FITRIANI SITUMORANG Anggreandari, Rizky Ani Suryani Ani Suryani Anja Meryandini Antonius Suwanto Aris Tjahjoleksono Aris Tri Wahyudi Atang Sutandi Ayni, Qurrotu Bambang - Riyanto Besty Maranatha BRAMANTYO JATI PRASOJO Budiasih Wahyuntari Budiasih Wahyuntari Budiasih Wahyuntari Cahyadi, Alfan Dadang Suhendar Dadang Suhendar Delfi Trisnawati DERI YURATMOKO Desniar - - Dewi Seswita Zilda Dewi Seswita Zilda Dian Syahfitri Dinamella Wahjuningrum Dwi Ambarawati DWI ANDREAS SANTOSA Ekowati Chasanah Encah Ewi Mulyeti Esti Puspitasari ESTI PUSPITASARI Evi Damayanti Evi Damayanti, Evi Fauzy Rachman Fauzy Rachman Fauzy Rachman Ferymon Mahulette Ferymon Mahulette Ferymon Mahulette, Ferymon Fitratul Aini Fitratul Aini, Fitratul Fitriani Idham Galih Cendana Nabilasani Galih Cendana Nabilasani Hadi Susilo Hamim Hamim Hana Nurullita Prestisia Hasrul Satria Hasrul Satria, Hasrul HENDRA PARDEDE Hirmas Fuady Putra HIRMAS FUADY PUTRA, HIRMAS FUADY Idin Abidin Ika Roostika Tambunan, Ika Roostika Ika Setianingsih, Ika Iman Rusmana Iman, Rusmana Ismi Isti'anah Isna Rahma Dini Iswati, Ruma It Jamilah Ivan Permana Putra Jepri Agung Priyanto Jepri Agung Priyanto, Jepri Agung Karina Eku Dwinanda Gunawan Kusuma Handayani LAKSMI AMBARSARI Lia Siti Nur'amaliyah Lia Siti Nur'amaliyah Lisdar Idwan Sudirman Listyowati, Sri lmiah, Sitti Nur Luky Adrianto Maggy Thenawidjaya Suhartono Maherani, Vincentia Fenice Angger Maranatha, Besty Maria Dita Febriani Lumban Gaol Marini Adani Mashudi Mashudi Maslahah, Iah Novi Masrukhin Masrukhin Muhamad Azwar Syah Mulyorini Rahayuningsih Mutiha Panjaitan Nabilasani, Galih Cendana Nabilasani, Galih Cendana Ninda Ningtyas Nuraliah Rusman Nurfahmi, Riziq Ilham Nurul Hidayati Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Prayoga SURYADARMA Puspita Lisdiyanti Rika Indri Astuti RIMA ERNIA Risky Hadi Wibowo Risky Hadi wibowo Rizky Anggreandari Rury Eryna Putri Sarah Asih Faulina Sarah Asih Faulina, Sarah Asih Sipriyadi Sipriyadi Sipriyadi Siswa Setyahadi Siswodarsono, Trismilah Siswodarsono, Trismilah Siti Azzira Rahma Sitti Nur Ilmiah Sonya Tobing Sri Budiarti Poerwanto Sri Listyowati Suhendar, Dadang Suhendar, Dadang SYAMSUL BAHRI SYAMSUL BAHRI TEDJA IMAS Thenawidjaya, Maggy Titi Candra Sunarti TRI ASMIRA DAMAYANTI Tri Handayani Kurniati Tri Handayani Kurniati Trismilah Trismilah Siswodarsono Untung Sudadi Wahyu Widosari Wibowo, Risky Hadi Widanarni Widanarni WIDANARNI WIDANARNI Widosari, Wahyu Yoan Ramasita Yusro Nuri Fawzya Zaenal Arifin, Sukarya Zulfarina Zulfarina Zulfarina,

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