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Journal : Biotropika

Compatibility of Neem (Azadirachta indica) and Beauveria bassiana for Control of Spodoptera exigua and the Theoretical Impact to the Agroecosystem Kurnianto, Agung Sih; Prastiwi, Santi; Dewi, Nilasari; Tanzil, Ahmad Ilham; Muhlison, Wildan; Wagiyana, Wagiyana
Biotropika: Journal of Tropical Biology Vol. 10 No. 2 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.02.01

Abstract

Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae) is the biggest threat to onion farming, especially in the vegetative phase. In severe attacks, this pest can cause yield losses of up to 100%. Compatibility is one way to increase the efficiency and effectiveness of pest control by using plant-based pesticides or biological agents. Neem Leaf Extract (Azadirachta indica, acronym=NLE) has an azadirachtin compound that can inhibit insect growth, reduce appetite, reproduction, and hatch eggs. The fungus Beauveria bassiana (acronym=Bb) can secrete chitinase, lipase, proteinase enzymes that are able to decompose insect cuticles. NLE was obtained through the extraction method of plant-based pesticides and stored at 4oC until the experiment time. The Bb used was a commercial B. bassiana inoculum in the flour form with a density of 4.5 x 1010 spores/gram (trade name = Natural BVR). To determine the advantages of compatibility, this study is held by a single toxicity test of Bb and NLE, and the combined toxicity test of both. This study used a completely randomized design (CRD) consisting of 6 treatments with 4 replications, each replication used 12 larvae (a total of 48 larvae). Combination toxicity has a higher toxicity than the single use of NLE and Bb. At the LC 95 level, the combination treatment of Bb and NLE was 1.06-1.15 times more toxic than the single treatment of Bb, and 6.87-7.79 times more toxic than the single treatment of NLE. NLE and Bb are considered to have high compatibility (strong synergistic with GI value <0.5). Theoretically, the compatibility of NLE plant-based pesticides and Bb is very promising to replace chemical pesticides that have long-term adverse effects on agroecosystems.
Co-Authors . Usmadi A. F. Hidayatullah A. R. S. Ghozali Agung Sih Kurnianto Agung Sih Kurnianto Agung Sih Kurnianto Ahmad Ilham Tanzil Ahmad Ilham Tanzil Ahmad Ilham Tanzil Alfarisy, Fariz Kustiawan Anantoro, Tri Anggarini, Cindy Dwi Anggriawan, Rendy Ankardiansyah Pandu Pradana Antono, Moh Nuri Arlianto, Irfan Sarifuddin Arum, Ayu Puspita Arum, Ayu Puspita Bety Etikasari Bugar Syarif Ahmada Candra Setiawati, Tri Cindy Dwi Anggarini Dewi, Nilasari Dwi Erwin Kusbianto Dwi Maharani, Ariq Dyah Ayu Savitri Evita Soliha Hani Hadi, Yusnan Hari Purnomo Hari Purnomo Hari Purnomo HARI PURNOMO Haryadi, Nanang Hasbi Mubarak Hermanu Triwidodo Himmatul Khasanah Husni dan Zulfanazli Pudjianto Alfian Rusdy Ika Purnamasari Indah Ibanah Irwanto Sucipto Irwanto Sucipto, Irwanto Ismil Arum Fitri Asih Jadmiko, Wildan Khotijah Khotijah Laily Ilman Widuri, Laily Ilman Listya Purnamasari Luhur Septiadi Maria Azizah Maslucha, Nurul Muhammad Bagus Dirrar Al Ashar Muhammad Usman, Muhammad Nabilah Aulia Rohmah Nanang Tri Haryadi Nanang Tri Haryadi Nanda Khoirun Nisa Ahmad Nilasari Dewi Nur Laila Magvira nurul latifah Oria Alit Farisi Pamungkas, Yoga Anugrah Patricia SM, Susan Barbara Prastiwi, Santi Prayoga, Mohammad Candra Puji Rahayu Pusparani, Syafina Putri, Aprilia Pangestu Qorinatul Ulya Rachmandhika, Yusuf Rachmi Masnilah Resa Dwi Arianti Restanto, Didik Pudji Rina Kumalasari Ristiyana, Suci Rofiqi, Ahmad Rosyady, Muhammad Gufron Ryka Afhe Amela Safitri, Eva setiyono Setiyono Setiyono Sigit Soeparjono Syahputra, Wahyu Nurkholis Hadi Syakuro, Muhammad Yusuf Abdan Titin Agustina Tri Saputra, Tri Tri Wahyu Saputra Tri Wahyu Saputra, Yoga Tyas, Iswahyuning Ummi Sholikhah Wagiyana Wagiyana, Wagiyana Yagus Wijayanto Yoga Anugrah Pamungkas Yulianto, Roni Yusuf Rachmandhika Zahro, Fauziatuz