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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner JURNAL KIMIA SAINS DAN APLIKASI Medical Journal of Indonesia Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Biodidaktika : Jurnal Biologi dan Pembelajarannya Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Medik Veteriner ARSHI Veterinary Letters Berkala Penelitian Hayati Jurnal Kedokteran Hewan Jurnal Medika Veterinaria
Agustin Indrawati
Bagian Mikrobiologi Medik, Departemen Ilmu Penyakit Hewan Dan Kesmavet, Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Journal : Buletin Peternakan

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Application of Multiplex PCR Assay for Campylobacter fetus Sub sp. Detection and Differentiation in Bovine Preputial Wash Seruni Agistiana; Surachmi Setiyaningsih; Agustin Indrawati
Buletin Peternakan Vol 42, No 1 (2018): BULETIN PETERNAKAN VOL. 42 (1) FEBRUARY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i1.26114

Abstract

The aim of this research was to evaluate the detection and differentiation of C. fetus subspecies through multiplex PCR (mPCR) method from the bovine preputial wash. The evaluation was done by comparing 2 extraction methods, which was by commercial kit extraction and boiling method, and also by measuring the effect of Transport Enrichment Media (TEM) addition as bacterial enrichment. Spiked bovine preputial washes with 2 extraction methods (commercial kit and boiling), with and without TEM addition were used as a sample. The results showed that the highest sensitivity/specificity was by boiling extraction without TEM addition, which reached 83.3%/100% for all or grouped sample. The results also supported by Poisson regression analysis which showed the comparison between sample with and without TEM addition which had 1.3 Incidence Rate Ratio (IRR). The obtained IRR score indicated that sample without TEM addition had 30% better result compared to the sample with TEM addition. Furthermore, a comparison between commercial kit and boiling extraction showed no significant difference (1.029 IRR). The research concluded that boiling extraction without TEM addition is the most accurate, fast and economical extraction method regarding its high sensitivity and specificity value, with less than 24 hours testing time and without any commercial kit requirements. However, a validation and verification with field sample were still needed before applied in the laboratory.
Co-Authors . Darniati Aditya Primawidyawan Afifah Nurhasanah Afiff , Usamah agrita, tri wera Agus Sjahrurachman Ambar Retnowati Ambar Retnowati Angela Mariana Lusiastuti ANGELA MARIANA LUSIASTUTI ANGELA MARIANA LUSIASTUTI Apris Beniawan Ardiana Ardiana Arum, Diah Sekar Bayu Febram Prasetyo Betty Ernawati Damiana Rita Ekastuti Darniati D, Darniati Denny Widaya Lukman Destri Prameswari, Alvira Diah Sekar Arum Dwi Endrawati Edi Purwanto Elmanaviean Eni Kusumaningtyas Erdina Pangestika Erin Kurnianingtyas Fachriyan H Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadlilah, Ulfi Nurul Fera Ibrabim Guntari Titik Mulyani Hadri Latif Hamdika Yenri Handayani Halik Handina Rakhmawati HELGA SEEGER Hendarko, Sriani Hermana, Nabila Swarna Puspa Herwin Pisestiyani I Wayan T. Wibawan I wayan Teguh Wibawan Idwan Sudirman Ita Krissanti Jefri Naldi Jeni Maharani Julia Rosmaya Riasari Kuntum Khoirani Kurnia Tiara Aulia Lee Xia Meen Leila Nur Aziah Leila Nur Azizah Lila Gardenia Maharani, Jeni Mangaraja Pidoli Tampubolon Mardiastuti Mardiastuti MICHAEL ZSCHÖCK Mira Mawardi Mirnawati Bachrun Sudarwanto Mirnawati Sudarwanto Muh Ramadhan Nabila Swarna Puspa Hermana Nabila Swarna Puspa Hermana Nafiqoh, Nunak Naldi, Jefri Nauval Firdana, Chorrysa Navasuriya Radha Krisnan Nazmi Zahir B. Mohd Zaini Ni Luh Putu Ika Mayasari Ni Luh Putu Ika Mayasari Nining Arini Novita, Hessy Nurhasanah, Afifah Nurul Fadillah Nurul fadillah, Nurul Oktaviani, Dian Okti Nadia Poetri Penataseputro, Tanjung Pratitis S Wibowo Pratitis S Wibowo Rahmat Hidayat Rahmat Hidayat Ramadhaniah, Vetty Reinilda Alwina Retno D. Soejoedono Retno Damajanti Retno Damayanti Soejoedono Rifky Danial Rifky Rizkiantino Risqika Aqla Velayati Romsyah Maryam Rukmi, M.G. Isworo Ryan Septa Kurnia Safika S, Safika Sapto Andriyono Sartika Juwita Seruni Agistiana Setiadi Setiadi Siti Fatimah Siti Gusti Ningrum, Siti Gusti Siti Nur Jannah Sucitya Purnama Suhartila Suhartila Suherman . Surachmi Setiyaningsih Susan M Noor Susan M Noor Susanti, Wiwik Susanto Nugroho Syahidah, Dewi Teguh Budipitojo Titiek Sunartatie Titis Wulandari Tri Wiyoko Trioso Purnawarman Triwardhani Cahyaningsih Ulfi Nurul Fadlilah Uni Purwaningsih Uni Purwaningsih Upik Kesumawati Hadi Usama Affif Usamah Afif Uus Saepuloh Velayati, Risqika Aqla Wattiheluw, Muhammad Subhan Yan Evan Yenri, Hamdika Yuda Heru Fibrianto