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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology International Journal of Science and Engineering (IJSE) Journal of Aquaculture Management and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Jurnal Kelautan Tropis Omni-Akuatika Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Indonesian Fisheries Research Journal Journal of Aquaculture and Fish Health Jurnal Airaha Ilmu Perikanan Tropis Nusantara Jurnal Kedokteran Hewan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Jurnal Perikanan
Slamet Budi Prayitno
Aquaculture Study Program, Department Of Aquaculture, Faculty Of Fisheries And Marine Science, Diponegoro University, Semarang, Indonesia
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STUDI KASUS KEBERADAAN PENYAKIT IMNV (INFECTIOUS MYONECROSIS VIRUS) PADA UDANG VANAME (Litopenaeus vannamei) DI PERTAMBAKAN PEKALONGAN, JAWA TENGAH Sarah, Humidah; Prayitno, Slamet Budi; Haditomo, Alfabetian Harjuno Condro
Journal of Aquaculture Management and Technology Volume 6, Nomor 3, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Udang vaname (Litopenaeus vannamei) menjadi salah satu spesies andalan bagi pertambakan di indonesia. Salah satu kendala pada pembudidaya udang vaname saat ini adanya penyakit IMNV (Infectious Myonecrosis Virus). Pekalongan merupakan salah satu produksi udang vaname yang cukup penting di Jawa Tengah. Pemantauan penyakit penting seperti IMNV sangat perlu dilakukan untuk mengetahui potensi resiko tertular dan tersebarnya penyakit IMNV di Pekalongan. Peneltian ini bertujuan untuk mengkaji status kesehatan udang khususnya dari infeksi IMNV pada tambak intensif di Kota Pekalongan. Metode penelitian yang dilakukan adalah menggunakan metode studi kasus, dengan melakukan pengambilan sampel udang melalui purposive random sampling dan wawancara kepada pemilik tambak. Jumlah udang 144 ekor dari 8 tambak terpilih. Hasil real time PCR dan analisa histopatologi terhadap organ daging bagian ekor menunjukkan bahwa 25% tambak contoh terinfeksi IMNV yaitu di desa Krapyak dan desa Kandang Panjang, sedangkan hasil histopatologi menunjukkan bahwa jaringan daging udang mengalami nekrosis. Berdarsarkan hasil diatas, tambak intensif di Kota Pekalongan memiliki potensi terinfeksi IMNV pada tahap sedang. Vaname shrimp (Litopenaeus vannamei) became a prime species cultivated in brackish water  ponds in Indonesia. The problem that are often found in field, is IMNV (Infectious Myonecrosis Virus) disease. Pekalongan district is one of important shrimp produces in Central Java. Disease monitoring program is an importanted step that should be carried out especially for IMNV infection. This was to evaluate the potential disease infection and spread into the culture area. The aim of this research was to study the health status of intensive shrimp culture in Pekalongan with regard IMNV infection. 144 shrimp were randomly selected brackish water intensive ponds. The sample were then analysed by real time PCR to correct the present of IMNV. Histopathologycal study was also cound. The research showed that 25% of intensive shrimp ponds samples were infected by IMNV. Furthemore, their caudal flesh demmostrated necrosis. This can be concluded that shrimp ponds in Pekalongan mildly risk of IMNV.
PENGARUH KONSENTRASI KONSORSIUM BAKTERI K7, K8 DAN K9 TERHADAP STATUS KESEHATAN RUMPUT LAUT (Eucheuma cottonii) Raharja, Edward; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 5, Nomor 1, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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E. cottonii berperan sebagai penyumbang utama produksi perikanan. Salah satu faktor penghambat produksi adalah penyakit ice-ice. Penyakit ini disebabkan oleh infeksi bakteri patogen terhadap thallus. Penelitian ini bertujuan untuk mengetahui gejala klinis rumput laut (E. cottonii) yang terinfeksi konsorsium bakteri dan Mengetahui pengaruh tingkat konsentrasi bakteri penyebab timbulnya penyakit ice-ice pada rumput laut (E. cottonii). Metode pada penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuannya dengan konsorsium bakteri K7,K8 dan K9 yang sama dan konsentrasi yang berbeda yaitu A (106 CFU/ml), B (107 CFU/ml), C (108 CFU/ml), dan D (tanpa inokulasi/kontrol).  E. cottonii bobot 1-1,5 gram dan panjang  5 cm dipelihara dalam botol kaca yang diisi air laut steril 200 ml selama 9 hari. Kondisi suhu 28°C, salinitas 30 ‰, dan pH 7. Pemeliharaan menggunakan shaker kecepatan 100 rpm. Hasil penelitian menunjukkan konsentrasi konsorsium K7, K8 dan K9 yang berbeda berpengaruh nyata (P < 0,05) terhadap nilai pertumbuhan mutlak E. cottonii. Gejala klinis yang ditimbulkan adalah munculnya spot putih yang kemudian akan bertambah panjang dan mengakibatkan thallus patah. Bakteri konsorsium yang menyebabkan gejala klinis tersebut adalah Corynebacterium sp., Baccilus sp., dan Alteromonas sp.. Berdasarkan hasil penelitian disimpulkan bahwa konsorsium bakteri K7, K8 dan K9 dengan konsentrasi 106 CFU/ml sudah mampu menimbulkan gejala klinis terhadap rumput laut uji. E. cottonii is one of a major contributor to the fisheries production. One of the inhibiting production factor is due to ice-ice disease. It is caused by several bacterial pathogens against seaweed thallus. The purposes of this research were to discover the clinical signs of seaweed (E. cottonii) that infected by bacterial consortium and the effect of the concentration levels of disease-causing bacteria ice-ice on seaweed (E. cottonii). The method in this study using a completely randomized design (CRD) with 4 treatments and 3 replications. Treatments were exposure of bacterial consortium K7, K8 and K9 at different concentrations namely A (106 CFU / ml), B (107 CFU / ml), C (108 CFU / ml), and D (without inoculation / control) to E. cottonii at 1-1,5 gram and 5 cm long was preserved in a glass jar filled with 200 ml of sterile sea water for 9 days. Bioecological condition was kept at 28 ° C, salinity 30 ‰, and pH 7 by using shaker at 100 rpm. The results showed that concentrations of a consortium of K7, K8 and K9 significantly different (P <0.05) to the absolute growth of E. cottonii. Clinical signs were ebserved by the appearance of white spots at the edge of thallus and spread up to along the thallus and end up with the broken thallus. Consortium of bacteria that cause the clinical signs were Corynebacterium sp., Baccilus sp., And Alteromonas sp.. Based on the results above it can be concluded that the bacterial consortium K7, K8 and K9 with a concentration of 106 CFU / ml are already capable of causing the clinical sign of ice-ice to seaweed experimental.
KARAKTERISASI BAKTERI DAN GAMBARAN HISTOPATOLOGI PADA IKAN GURAMI (Osphronemus gouramy) YANG TERSERANG PENYAKIT “MATA BELO” Latifah, Anisa Dwiaryani; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Mata belo adalah salah satu penyakit yang menyerang budidaya ikan gurami. Penelitian ini bertujuan mengetahui gejala klinis dan karakterisasi bakteri serta gambaran histopatologi ginjal, hati dan mata pada ikan gurami yang terserang penyakit “mata belo”. Metode yang digunakan pada penelitian ini adalah metode eksploratif, sedangkan metode pengambilan sampel menggunakan metode purposive sampling. Ikan gurami yang digunakan untuk isolasi dan histopatologi berukuran 16,8±3,4 cm sebanyak 7 ekor. Organ yang diisolasi yaitu ginjal, hati, mata, geripis dan luka. Isolasi bakteri menggunakan media Tryptic Soy Agar (TSA) dan Glutamate Starch Phenile (GSP). Isolat dipilih dengan melakukan seleksi berdasarkan morfologi koloni untuk uji reinfeksi. Ikan gurami yang digunakan sebagai ikan uji untuk uji reinfeksi berukuran 7 – 9  cm sebanyak 10 ekor per akuarium. Penyuntikan dilakukan dengan kepadatan bakteri 108 CFU/mL dan dosis 0,1 mL. Identifikasi bakteri dengan uji biokimia dan morfologi bakteri. Organ yang diamati saat uji histopatologi yaitu ginjal, hati dan mata. Hasil penelitian menunjukkan gejala klinis seperti exopthalmia, ekor geripis, luka pada tubuh dan hemorrhagic pada insang. Hasil isolasi didapatkan 28 isolat bakteri. Seleksi berdasar morfologi koloni bakteri didapatkan 7 isolat bakteri (NG01, NG06, NG07, NG11, NG13, NG15 dan NG14) untuk uji reinfeksi. Hasil uji reinfeksi menunjukkan bahwa 7 isolat bakteri mengakibatkan tingkat kematian ikan uji yang beragam yaitu 96,67%; 80,00%; 63,33%; 60,00%; 53,33%; 50,00%; 46,67%. Bakteri yang menyebabkan penyakit “mata belo” adalah bakteri Aeromonas hydrophila, sedangkan bakteri yang berasosiasi dengan penyakit “mata belo” adalah Staphylococcus aureus, Plesiomonas sigeloides, Escherichia vulneris dan Bacillus mycoides. Hasil pengamatan histopatologi ditemukan kelainan yaitu kongesti, nekrosis dan degenerasi pada ginjal, kongesti, melanomakrofag dan degenerasi pada hati serta nekrosis pada mata. Mata belo is one of the fish diseases that infect the culture of gouramy. The aim of this study to determine the clinical sign of the disease and to know histopathology characterization of kidney, liver and eye. The method in this research used sampling method with purposive sampling method. The gouramy samples to used isolation and histopathology have length 16.8±3.4 cm. Isolation of bacteria using media Tryptic Soy Agar (TSA) and Glutamate Starch Phenile (GSP). Isolated organs are kidneys, liver, eye, and wound. Isolates selected based on colony morphology for reinfection test. Gouramy samples for reinfection test have length 7 – 9 cm. The density of injection 108 CFU bacteria/mL with dose 0.1 mL. Identification of bacteria based from biochemical and morphological criteria. Histopathology organs were kidneys, liver and eye. The results of the clinical sign was a exopthalmia and hemorrhagic in the gills. Results obtained 28 isolates of bacterial isolation. The isolat was selected 7 isolates (NG01, NG06, NG07, NG11, NG13, NG15 and NG14) to reinfection test. The test results showed that mortality in 7 isolates were 96.67%; 80.00%; 63.33%; 60.00%; 53.33%; 50.00%; 46.67%. The bacteria was caused on “mata belo” is Aeromonas hydrophila, while the bacteria was associated on “mata belo” are Aeromonas hydrophila, Staphylococcus aureus, Plesiomonas sigeloides, Escherichia vulneris and Bacillus mycoides. Histopathology results were congestion, necrosis and degeneration of the kidneys, while in the liver was congested, melanomacrofag, degeneration and necrosis at the eye.
PEMANFAATAN EKSTRAK DAUN BINAHONG (Anredera cordifolia) UNTUK PENANGGULANGAN PENYAKIT BAKTERI (Vibrio harveyi) PADA UDANG WINDU Pratama, Pungki Nanda; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Penyakit bakterial merupakan kendala yang menyebabkan penurunan produksi seperti halnya vibriosis. Vibriosis merupakan penyebab kematian utama pada larva, post larva, juvenil, remaja dan udang dewasa hampir mendekati 100%. Salah satu jenis vibrio yang sering menyebabkan kematian pada udang windu adalah Vibrio harveyi. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penggunaan ekstrak daun binahong (A. cordifolia) dan mengetahui dosis terbaik terhadap kelulushidupan dan histopatologi udang windu yang diinfeksi V. harveyi. Penginfeksian V. harveyi sebanyak 0,1 mL dengan dosis 105 CFU/ml pada bagian intramuskular.Pada penelitian ini digunakan konsentrasi ekstrak daun binahong dengan dosis perlakuan  0 ppm, 600 ppm, 800 ppm dan 1000 ppm, kemudian dilakukan perendaman (dipping) selama 8 menit. Dari treatment perendaman ekstrak binahong memberikan pengaruh nyata terhadap kelulushidupan dan histopatologi hepatopankreas udang windu. Treatment A (600 ppm) merupakan dosis terbaik untuk mengobati udang windu yang terinfeksi V. harveyi. Bacterial disease is the one problems causes a decreasing production like as vibriosis. Vibriosis is the main problem cause mortality in larvae, post-larvae, juvenile, and adult stages of shrimp close to 100%. Vibrio harveyi is one of species from genus vibrio which often causes mortality in black tiger shrimp. The aim of this resaerch to determine the effect of utiliztion of binahong leaf extracts (A. cordifolia) and determine the best dose to survival and histopathological of tiger shrimp it was infected by V. harveyi. V. harveyi injection as much as 0.1 mL with a dose of 105 CFU / ml in the intramuscular tissue. The research used concentration of Binahong leaf extract in four treatment were 0 ppm, 600 ppm, 800 ppm, and 1000 ppm, then carried to immersion treatment during 8 minutes. Based from immersion of binahong extract has significant effect on survival and hepatopancreas histopathology of Tiger shrimp. Treatment A (600ppm) is the best dose to cure V. harveyi infection in tiger shrimp.
Ketahanan Nonspesifik Ikan Mas (Cyprinus Carpio) Yang Direndam Ekstrak Daun Jeruju (Acanthus ilicifolius) Terhadap Infeksi Bakteri Aeromonas hydrophila Dianti, Lusi; Prayitno, Slamet Budi; Ariyati, Restiana Wisnu
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
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Abstract

This research was aimed to study the clinical symptoms of fish infected by Aeromonas hydrophila and examine the effect of leaf extract Acanthus ilicifolius usage through the method of soaking to prevent Motile Aeromonas Septicemia disease. 96 fish was soaked with leaf of extract A. ilicifolius for 30 minutes. The challenge was done by injecting of bacterial suspensions Aeromonas hydrophila with 108 CFU/ml density intra-muscularly to the fish. The results showed clinical symptoms of moribund fish such as moving slowly, swimming on the surface, swim upsided, expothalmia, ulcer and accumulation of fluid in the abdominal cavity. Further results showed that extract of A. ilicifolius leaf did not significantly protected (P>0.05) the survival of tested fish. Observations of blood profile after infection showed the average from the highest to lowest total erythrocytes were C (500 ppm) of 2.69 x 106 cells/mm3, B (300 ppm) 2.31 x 106 cells/mm3, A (0 ppm) 2.29 x 106 cells/mm3 and D (700 ppm) 2.18 x 106 cells/mm3 recpectively. The average of the total leukocytes from the highest to lowest D (700 ppm) of 3.63 x 104 cells/mm3, C (500 ppm) 3.60 x 104 cells/mm3, B (300 ppm) 3.53 x 104 cells/mm3 and A (0 ppm) 3.47 x 104 cells/mm3 recpectively. The average value of the levels of highest to lowest hematokrit C (500 ppm) of 22.53%, D (700 ppm) 21,33% B (300 ppm) 20,67% and A (0 ppm) 20.33% recpectively. Leaf extract A. ilicifolius with dosage up to 700 ppm had not been able to improve imunne respone against infections of A. hydrophila
PENGARUH PERENDAMAN EKSTRAK DAUN BINAHONG (Anredera cordifolia) TERHADAP MORTALITAS DAN HISTOLOGI HATI IKAN MAS (Cyprinus carpio) YANG DIINFEKSI BAKTERI Aeromonas caviae Salikin, Rusydina Qamarul; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Ikan mas (Cyprinus carpio) adalah salah satu komoditas perikanan air tawar yang mempunyai nilai ekonomis yang tinggi. Adanya serangan penyakit pada budidaya ikan mas seperti bakteri A. caviae, dapat menyebabkan kerugian ekonomi dalam budidaya. Banyak upaya yang dilakukan untuk mencegah dan mengobati serangan bakteri A. caviae, salah satunya menggunakan fitofarmaka ekstrak daun binahong. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan ekstrak daun binahong (A. cordifolia) serta mengetahui dosis yang terbaik terhadap kematian dan histologi ikan mas yang diinfeksi bakteri A. caviae. Ikan  mas (C. carpio) yang digunakan sebanyak 96 ekor  kemudian disuntik bakteri A. caviae dengan kepadatan 107 CFU/mL secara intramuskular. Pelarut yang digunakan dalam ekstrak binahong menggunakan aquades. Perendaman dengan ekstrak daun binahong dilakukan setelah 2 hari pasca penyuntikan. Nilai rata-rata mortalitas tertinggi hingga terendah berturut-turut yaitu 83,86% (perlakuan A), 68,86% (perlakuan B), 57% (perlakuan C) dan 55,78% (perlakuan D). Perendaman dengan ekstrak daun binahong menunjukkan hasil yang berpengaruh nyata (P<0,05) hal ini terbukti dari semakin tingggi dosis ekstrak yang digunakan, semakin rendah kematian pada ikan mas. Hasil pengamatan gambaran histopatologi diperoleh adanya kerusakan berupa nekrosi, degenerasi dan melanomakrofag pada organ hati. Oleh karena itu perendaman ekstrak daun binahong mampu memberikan efek penyembuhan terhadap ikan mas yang diinfeksi bakteri A. caviae. Carp (Cyprinus carpio) is one of the freshwater aquaculture commodities which have high economic value. Bacterial Disease caused by Aeromonas caviae often infected Carp culture. Many efforts made to prevent infection by A. caviae bacteria, one of which used phytopharmaca binahong leaf extract. This aims of this research to determine the effect of the use of binahong ( A. cordifolia ) leaf extract and determine the effective dose on mortality and histology of carp was  infected by bacteria A. caviae. Carp ( C. carpio ) were used as much as 96 fish and that was injected by bacteria A. caviae with density 107 CFU / mL intramuscularly. Solvents used in binahong extract using distilled water. Soaking with leaf extract binahong done after 2 days post- injection. The results showed the average value of the highest to the lowest mortality in a row that the 83.86 % (treatment A), 68.86 % (treatment B), 57 % (treatment C) and 55.78 % (treatment D). Soaking with binahong leaf extract showed a significant effect ( P<0.05 ) on mortality. The results of histological observation of the liver and kidneys showed damage in the form of necrosis, degeneration and melanomacrofag in the liver. Soaking binahong leaf extract at a dose that has been able to provide a cure for carp infected bacteria A. caviae.
PENGARUH PEMBERIAN EKSTRAK DAUN JERUJU (Acanthus ilicifolius) TERHADAP PROFIL DARAH IKAN KERAPU MACAN (Epinephelus fuscoguttatus) Agung, Lukman Anugrah; Prayitno, Slamet Budi; -, Sarjito
Journal of Aquaculture Management and Technology Vol 2, No 1 (2013) : Journal of Aquaculture Management and Technology
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Abstract

ABSTRAK Ikan kerapu termasuk salah satu komoditas perikanan laut bernilai ekonomis penting. Ikan kerapu macan memiliki beberapa keunggulan diantaranya nilai gizi yang tinggi dan pertumbuhan yang lebih cepat daripada ikan kerapu jenis lain. Salah satu permasalahan dalam budidaya ikan kerapu macan adalah serangan penyakit. Salah satu cara untuk mencegah terjadinya penyakit ialah meningkatkan imunitas ikan. Daun jeruju (Acanthus ilicifolius) merupakan bahan herbal yang diduga mampu meningkatkan imunitas ikan kerapu macan. Peningkatan imunitas ikan dapat diketahui dengan memeriksa profil darahnya. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak daun jeruju (Acanthus ilicifolius) terhadap profil darah ikan kerapu macan meliputi jumlah eritrosit, jumlah leukosit, diferensial leukosit dan aktivitas fagositosis. Rancangan percobaan menggunakan Rancangan  Acak Lengkap  (RAL)  dengan  4  perlakuan  dan  3  ulangan. Dosis ekstrak daun jeruju yang digunakan adalah: perlakuan A (0 gr/kg); perlakuan B (5 gr/kg); perlakuan C (10 gr/kg); dan perlakuan D  (15  gr/kg). Pemberian ekstrak daun jeruju melalui pakan dilakukan selama 14 hari. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun jeruju berpengaruh nyata terhadap jumlah eritrosit (P<0,05) dan berpengaruh sangat nyata terhadap jumlah leukosit (P<0,01) namun tidak berpengaruh nyata terhadap diferensial leukosit dan aktivitas fagositosis ikan kerapu macan (P>0,05). ABSTRACT Grouper is one of economically commodity fish. Tiger grouper has several advantages including high nutritional value and rapid growth than other types of grouper. One of the problems in the tiger grouper culture  is a disease. One way to prevent the disease is to improve the immunity of fish, Jeruju leaf extracts is one of the herbal material which can increased fish imunity. Increased of fish immunity can be detected by examining of blood profile. The purpose of this experiment is determine the effect of jeruju leaf extract  (Acanthus ilicifolius) on the blood profile of tiger grouper i.e. : number of erythrocyte, leukocyte, differential leukocyte, and phagocyty activity. The design of the experiment is a completely randomized design (CRD) with 4 treatments and 3 replications. Jeruju (acanthus ilicifolius) leaf extracts were mixed with the artificial feed at concentrations of 0 g/kg ,5 g/kg, 10g/kg and 15 g/kg and  had been fed to grouper for 14 days period. The results showed that administration of jeruju leaf extract significantly effect on the number of erythrocytes (P<0.05) and highly significant effect on  the number of leukocytes (P <0.01) but no significant effect (P>0,05) on differential leukocyte, and phagocyty activity of tiger grouper.
IDENTIFIKASI AGENSIA PENYEBAB VIBRIOSIS PADA PENGGEMUKAN KEPITING BAKAU (Scylla serrata) DI PEMALANG Feriandika, Ferdian Bagus; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Vol 3. No 2 (2014): Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Vibriosis adalah problem utama pada pembesaran krustasea khususnya pada pembesaran kepiting bakau. Penelitian ini bertujuan mengetahui gejala klinis kepiting bakau yang terserang vibriosis dan mengetahui agensia penyebab vibriosis pada kepiting bakau. Metode pada penelitian ini adalah metode eksploratif dan metode pengambilan sampel menggunakan metode purposive sampling. Isolasi bakteri menggunakan media Thiosulphate Citrate Bile Salt Agar (TCBSA). Organ yang diisolasi yaitu hepatopankreas, insang, hemolymph dan luka pada karapas. Isolat dilakukan seleksi berdasarkan morfologi koloni untuk dilakukan uji postulat koch.  Penyuntikan dilakukan pada ruas kaki ke lima dengan kepadatan bakteri 108 CFU/mL dan dosis 0,1 mL. Identifikasi bakteri dilakukan dengan kriteria uji biokimia dan morfologi bakteri. Hasil penelitian menunjukan bahwa gejala klinis kepiting bakau yang terserang vibriosis adalah terdapat bercak merah pada karapas, luka pada capit dan abdomen serta kondisi kepiting yang lemah. Hasil isolasi didapatkan 19 isolat bakteri. Seleksi bedasarkan morfologi koloni bakteri didapatkan 5 isolat bakteri (SJP2, SJP3, SJP7, SJP10 dan SJP15) untuk dilakukan uji postulat koch. Hasil uji postulat koch menunjukan 5 isolat bakteri bersifat patogen terhadap kepiting bakau. Bakteri dari 5 isolat mengakibatkan kematian 100% dalam waktu kurang dari 12 jam. Agensia penyebab vibriosis pada kepiting bakau di Pemalang adalah Vibrio harveyi (SJP2), Vibrio cholerae (SJP3), Vibrio parahaemolyticus (SJP7), Vibrio alginolyticus (SJP10) dan Vibrio fischeri (SJP15). Vibriosis is a main problems in crustacean farming esspecialy  in an on growing mud crab (Scylla serrata). The aims of this research were to determine the clinical sign of mud crab that was infected by vibriosis and to  know bacterial agent of vibriosis in mud crabs. The method in this research was exploratory using purposive sampling method. The bacterial culture media used in the isolation was Thiosulphate Citrate Bile Salt Agar (TCBSA) medium. The bacterial isolates were spread on to TCBS medium for 24 hours. Isolat were guined from hepatopancreas, gills, hemolymph and injured carapace of the moribund mud crabs. The selected isolates were recultured in zobell liquid medium. The on colony morphology for postulates koch's test. The bacterial was injected on fift segments of swimming feet with bacterial density of 108 CFU/mL and 0.1 mL. Identification of bacteria carried by biochemical and morphological criteria of the bacterial test. The results showed that the clinical sign of mud crab was infected vibriosis, there are  red spots on the carapace, wounds in the abdomen, claws and crab weak conditions. From the moribund was obtained 19 isolates. Based on bacterial colony morphology it were obtained 5 colonies namely SJP2, SJP3, SJP7, SJP10 and SJP15 for  postulates koch's. The postulates koch's results showed that 5 isolates were pathogenic for mud crabs. The Bacteria from 5 isolates caused 100% mortality in less than 12 hours, based  this results bacterial  agents caused vibriosis in mud crabs cultur in Pemalang was  Vibrio harveyi (SJP2), Vibrio cholerae (SJP3), Vibrio parahaemolyticus (SJP7), Vibrio alginolyticus (SJP10) and Vibrio fischeri (SJP15).
SENSITIVITAS BAKTERI YANG BERASOSIASI DENGAN PENYAKIT IKAN LELE DUMBO (Clarias gariepinus) TERHADAP BERBAGAI MACAM OBAT IKAN YANG BEREDAR DI KABUPATEN PATI Suwarno, Yelliana Fatmawati; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Intensifikasi budidaya ikan lele dumbo (Clarias gariepinus) dapat memicu timbulnya penyakit bakterial.  Penelitian ini bertujuan untuk mengetahui agensia penyebab penyakit bakterial serta sensitivitasnya terhadap berbagai macam obat ikan yang beredar di pasaran.  Penelitian ini dilakukan dengan metode eksploratif dengan pengambilan sampel secara purposive random sampling.  Isolasi bakteri menggunakan media TSA, GSP, dan TCBS dengan metode streak pada luka, hati dan ginjal ikan.  Identifikasi bakteri dilakukan dengan kriteria uji biokimia dan morfologi bakteri.  Uji sensitivitas obat dilakukan secara in vitro.  Obat beredar yang digunakan yaitu; obat B™ dan C™ dengan dosis 2,5 µl, 5 µl dan 7,5 µl; obat A™ dengan dosis 6 µl, 12 µl dan 18 µl; serta obat D™ dengan dosis 2 µl, 4 µl, dan 6µl.  Hasil penelitian menunjukkan bahwa gejala klinis lele dumbo yang terserang bakteri terlihat dengan ciri – ciri  warna badan kuning, sirip geripis, terdapat luka (borok), dan warna tubuh pucat.  Hasil isolasi diperoleh 37 isolat bakteri. Seleksi berdasarkan morfologi koloni bakteri didapatkan 10 isolat bakteri untuk dilakukan uji selanjutnya.  Agensia penyebab penyakit pada ikan  lele dumbo dari Pati adalah Vibrio logei (LNH 5.2), V. fischeri (LNL 2.8), V.  furnishi (LNL 7.3), V. vulnificus (LNL 4.5), Aeromonas caviae (LNL 5.13), A. hydrophilla (LJ 3 dan LT 3), A. salmonisida (LTG 10 10’5) dan Edwardsiella ictaluri (LJ 5 dan LNH 8.17).  Hasil dari uji sensitivitas menunjukkan bakteri tersebut resisten terhadap A™, B™, C™ dan D™.  Hal ini berarti bahwa kesepuluh agensia penyebab penyakit bakteri pada ikan lele tidak sensitif terhadap obat beredar. Bacterial disease in catfish (Clarias gariepinus) due to intensification of aquaculture.  The aims of this study were to determine the agent which causing bacterial diseases and their sensitivity against common fish medicine.  The method used on this study was explorative with purposive random sampling.  Bacterial isolation using TSA, GSP and TCBS with streak method on wound, liver and kidney of fish.  Bacterial identification was performed using biochemical test qualification and the bacterial morphology.  Sensitivity test for common fish medicine was conducted through in vitro.  Common fish medicines which used were B™and C™ using  2,5 µl, 5 µl and 7,5 µl of dose; A™  using 6 µl, 12 µl and 18 µl of dose; and dusing 2 µl, 4 µl and 6µl of dose.  The material used 30 samples with lenght, then samples have baeter (15 – 24 cm) from Pati, Central Java.  The results showed that bacteria isolation obtained 37 isolates.  By morphological characters of bacterial colony was obtained 10 bacterial isolates which were selected for the further test.  Bacterial agents which  causing disease on catfish originated from Pati were Vibrio logei (LNH 5.2), V. fischeri (LNL 2.8), V. furnishi (LNL 7.3), V.  vulnificus (LNL 4.5), Aeromonas caviae (LNL 5.13), A. hydrophilla (3 LJ and LT 3), A. salmonisida (LTG 10 10'5) and Edwardsiella ictaluri (LJ 5 and LNH 8.17).  Mean while the sensitivity tests showed that all bacterias were not sensitive againts A™, B™,C™ and D™.
PATOGENISITAS DAN SENSITIVITAS AGENSIA PENYEBAB PENYAKIT BAKTERIAL PADA IKAN GURAMI (Osphronemus gouramy) TERHADAP BERBAGAI MACAM OBAT BEREDAR Yanti, Nuri Nia; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 4, Nomor 3, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Kebutuhan pangan nasional mengharapkan ketersediaan ikan gurami (Osphronemus gouramy) pada tahun 2015 sebanyak 26,005 ton. Hal ini mendorong para pembudidaya untuk mengoptimalkan hasil produksi ikan gurami. Namun seiring dengan berjalannya kegiatan budidaya, muncul banyak kendala yang dapat menurunkan hasil produksi, salah satunya ialah serangan penyakit bakteri. Untuk menanggulangi penyakit bakteri tersebut dilakukan pengobatan dengan menggunakan obat-obatan beredar. Tujuan penelitian ini adalah untuk mengetahui agensia penyebab penyakit bakteri yang menginfeksi ikan  gurami, mengetahui sensitivitasnya terhadap tiga macam obat beredar dan gejala klinis ikan gurami pasca penyuntikan bakteri melalui uji patogenisitas. Isolasi dilakukan pada 10 ekor ikan sampel yang berasal dari Banjarnegara pada hati, ginjal, mata dan luka pada media TSA (Tryptone Soy Agar). Dosis obat yang digunakan pada uji sensitivitas sesuai dengan anjuran dalam kemasan, sedangkan kepadatan bakteri yang digunakan pada uji patogenisitas yaitu 108 CFU/ml sebanyak 0,1 ml. Hasil isolasi diperoleh 21 isolat (NF 1, NF 2, NF 3, NF 4, NF 5, NF 6, NF 7, NF 8, NF 9, NF 11, NF 12, NF 13, NF 14, NF 15, NF 16, NF 17, NF 18, NF 20, NF  21, BSJ 6, BSJ 14), kemudian berdasarkan karakter morfologi dilakukan uji sensitivitas 11 isolat terhadap obat uji. Hasil uji sensitivitas isolat NF 1, NF 2, NF 6, NF 7, NF 8, NF 9, NF 11, NF 12, BSJ 6 dan BSJ 14 sensitive terhadap obat C, kecuali NF 16 bersifat resistence. Sedangkan terhadap obat A dan B isolat NF1, NF2, NF 6, NF 7, NF 8, NF 9, NF 12, NF 16 dan BSJ 6 bersifat resistence, kecuali NF 11 bersifat intermediate. Uji biokimia 6 isolat didapatkan bakteri Aeromonas hydrophilla (NF 6 dan NF 8), Enterobacter agglomerans (NF 11 dan BSJ 6), Staphylococcus aureus (NF 16) dan Aeromonas jandaei (BSJ 14), yang kemudian dilakukan uji patogenisitas. Hasil uji patogenisitas ditunjukkan dengan kemunculan gejala klinis berupa borok pada tubuh (A. hydrophilla), tubuh menghitam/gelap (E. agglomerans), exopthalmia/mata menonjol (S. aureus), sedangkan A.jandei tidak ditemukan gejala klinis spesifik. National food’s requirement expect, the availability of gouramy (Osphronemus gouramy), were increased 26.005 ton in 2015. Those requirement encourage fish farmer to optimize gouramy production. In the same time there are many problems that can decrease gouramy production, one of them is a diseases caused by pathogenic bacteria. Treatment, for that usually use artificial medicine that sold in the market. The purpose of this research was to know the causative agents of bacterial disease that infected gouramy and to know bacterial sensitivity to three commercial medicines and to know clinical sign of gouramy that bacteria injection by patogenisity. There were ten moribund fish’s taken from Banjarnegara with target organ were liver, kidney, eye and wound. Bacteria from those organs were isolated on TSA (Tryptone Soy Agar) Media. Medicine dosage for sensitivity as recommended in the package, and bacterial’s density for patogenisity was 108 CFU/mL : 0,1 mL. Isolation of 4 organs revealed. Eleven isolate were selected for sensitivity test out of using twenty one isolates, (NF 1, NF 2, NF 3, NF 4, NF 5, NF 6, NF 7, NF 8, NF 9, NF 11, NF 12, NF 13, NF 14, NF 15, NF 16, NF 17, NF 18, NF 20, NF 21, BSJ 6 dan BSJ 14).  Three commercial medicine A, B, and C, were exposed to those eleven isolates. Sensitivity test revealed that all 11 isolates (NF 1, NF 2, NF 6, NF 7, NF 8, NF 9, NF 11, NF 12, BSJ and BSJ 14) sensitive to medicine C. Only isolate NF 16 was resistence. To medicine A and B, while others resistence. moreover isolates NF 11 was intermediate. Marphology and biochemical test of 6 isolates revealed that there were : Aeromonas hydrophilla (NF 6 and NF 8), Enterobacter agglomerans (NF 11 dan BSJ 6), Staphylococcus aureus (NF 16) and Aeromonas jandaei (BSJ 14). Patogenisity test confirmed 6 bacteria were pathogenisc with clinical signs as follows : ulcer on body (A. hydrophylla), exopthalmia (S. aureus), blackenedat on body (E. agglomerans) and no specific clinical sign (A. jandaei).
Co-Authors - Aminah - Desrina, - - Istikhanah - Susanti, - - Triyaningsih Adhi Kurniawan Agatya Sara Ardiantami, Agatya Sara Agil Setya Utomo, Agil Setya Agung Sudaryono Agus Sabdono Agustina Agustina Agustina Alfabetian Condro Haditomo Alfabetian Harjuna Condro Haditomo Alfabetian Harjuno Condro Haditomo Amanda Mega Putri, Amanda Mega Ambaryanto Ambaryanto Amelia Rimadhani ANGELA MARIANA LUSIASTUTI Anggun Putriani Situmorang, Anggun Putriani Aninditia Sabdaningsih Anisa Dwiaryani Latifah Annisa Amalia Awanis Aprilia Dwi Indriani Ardulanisa, Ridha Arif, Gunarso Arifianto Heraedi Asep Akmal Aonullah Asriyana, . Aulia Ayu Ermantianingrum Aulia Resty Wijayanti Ayi Santika Ayu Wulandari Azis Nur Bambang Bagus Oktori Sutrisno cahyadi, jimmy Chyntia Arindita Dani Indrarini Desrina Desrina Desrina, - - Devitha Tri Utami Dewi Nurhayati Dian Ratna Sari Didik Wahju Hendro Tjahjo Edward Raharja, Edward Endah Setyowati Eni Ashfa Ashofa Fajar Purnama, Muhammad Ferdian Bagus Feriandika Fifiana Zulaekah fikih rosalina pratiwi Frida Purwanti Gina Saptiani Gina Saptiani Haditomo, A.H. Condro Hardyta Noviar Rahma Hariyadi, Putut Hasna, Salma Khoironnida Hendrawan, Andika Luky Setiyo Ida Ayu Putu Sri Widnyani Ikhsan Pratama Indah Febry Hastari Intan Eska Amalia Syahida Johannes Hutabarat Kamil, Mia Rahmah Khaerani, Laela Rizki Kurniawan Kurniawan Kusuma, Winanda Adi Laksono Trisnantoro Lestari Lakhsmi Widowati Linuwih Aluh Prastiti Lukman Anugrah Agung Lusi Dianti Marwenni Siregar, Marwenni Max Rudolf Muskananfola Mohammad Saleh Lubis Monica Nanda Muhammad Fajar Purnama Nailil Muna Nida Qolbi Salma Rochani Nita Amelia Nur Annisa Nuri Nia Yanti, Nuri Nia Nurmanita Rismaningsih Nurul Hidayati Ocky Karna Radjasa Peppy Dewi Fitriyanti Pinandoyo Pinandoyo Pramudita Apriliyanti Pricilia, Shella Pujiono Wahyu Purnomo Pungki Nanda Pratama Purnama, Muhammad Putut Har Riyadi R. Dewi Dharina Nurjannah Restiana Wisnu Ariyati Rismaningsih, Nurmanita Ristiawan Agung Nugroho Rohita Sari Rosa Amalia Rusda, Yusrotul Rusydina Qamarul Salikin Sahala Hutabarat Samsul Rizal Sapto P Putro Sarah, Humidah Sarjito - Sekar Ayu Chairunnisa Seto Windarto Shiddieqi, Hasbi Ash Siti Nurjanah Siti Ziyadaturrohmah Solly Aryza Sri Nuryati Sri Rejeki Subandiyono Subandiyono Subhan Subhan Sulisyaningrum Sulisyaningrum Sumbung, Sepri Suryanti Suryanto, Damang Sutrisno Anggoro Syakuri, Hamdan Teguh Eko Suryo Agil Hermawan Tristiana Yuniarti Triwulandari, Atita Utari, Viola Indah Vivi Endar Herawati Widiana, Giacinta Risti Windarto, Seto Yelliana Fatmawati Suwarno Yohanes Kristiawan Artanto YS Darmanto Yunia Dharmastuti Widyaningsih Yusuf Kamlasi Zairin Zain