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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology International Journal of Science and Engineering (IJSE) Journal of Aquaculture Management and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Jurnal Kelautan Tropis Omni-Akuatika Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Indonesian Fisheries Research Journal Journal of Aquaculture and Fish Health Jurnal Airaha Ilmu Perikanan Tropis Nusantara Jurnal Kedokteran Hewan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Jurnal Perikanan
Slamet Budi Prayitno
Aquaculture Study Program, Department Of Aquaculture, Faculty Of Fisheries And Marine Science, Diponegoro University, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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PENGARUH PERENDAMAN EKSTRAK DAUN SIRIH (Piper betle) DENGAN KONSENTRASI YANG BERBEDA TERHADAP GEJALA KLINIS, KELULUSHIDUPAN, HISTOLOGI DAN PERTUMBUHAN UDANG VANAME (Litopenaeus vannamei) YANG DIINFEKSI Vibrio harveyi Annisa, Nur; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Volume 4, Nomor 3, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Bakteri Vibrio harveyi merupakan salah satu agensia penyebab vibriosis yang dapat menyebabkan kematian pada udang, sehingga menimbulkan kerugian secara ekonomi. Tujuan dari penelitian ini adalah mengetahui pengaruh konsentrasi perendaman ekstrak daun sirih (Piper betle) berbeda terhadap gejala klinis, kelulushidupan, histologi dan pertumbuhan udang Vaname (L. vannamei) yang diinfeksi V. harveyidan mengetahui konsentrasi terbaik dari perendaman ekstrak daun sirih (Piper betle).Udang uji yang digunakan berjumlah 120 ekor dengan ukuran ±7 cm. Penginfeksian V. harveyi sebanyak 0,1 mL dengan konsentrasi 106 CFU/ml pada bagian intramuskular.Pada penelitian ini digunakan konsentrasi ekstrak daun sirih dengan konsentrasi perlakuan 0 ppm, 500 ppm, 800 ppm dan 1100 ppm. Gejala klinis yang muncul adanya warna memerah pada bagian ekor dan telson serta seluruh tubuh, perubahan warna kehitaman pada hepatopankreas serta udang terlihat pasif.  Perendaman (dipping) dilakukan selama 10 menit. Hasil penelitian diperoleh bahwa perendaman ekstrak daun sirih memberikan pengaruh nyata terhadap kelulushidupan dan histopatologi hepatopankreas udang vaname akan tetapi tidak berpengaruh nyata terhadap pertumbuhan udang vaname. Hasil ini juga diperoleh bahwa penggunaan konsentrasi1100 ppmmerupakan konsentrasiekstrak daun sirih terbaik untuk mengobati udang vaname yang terinfeksi V. harveyi. Vibrio harveyi isone of vibriosis agents that couldmass mortality in farmed shrimp, therefore result in significant losses in shrimp farming. The purpose of this researchis to find out the effect of various immersion concentration of betel leaves extract (Piper betle) toward clinical symptoms, survival rate, histology and growth of vannamei (L. vannamei) thatinfectedby V. harveyi and to determine the best concentration of immersion betel leaves extract (Piper betle).Infection was carried out by intramuscular infection of 0,1 ml of 106 CFU/ml V. harveyi. When clinical sign was shown redness on the tail and telson up the whole body flushed, discoloration and shrimp hepatopancreas blackened shrimp ponds passive then soaked with betel extract for 10 minutesThe result of the betel leavesextract immersion that give a significant effect on survival and histopathological hepatopancreas vaname but, it did not significantly affect the growth of shrimp vannamei. The result of this research which are the using concentration 1100 ppm is the best concentration to treat infected white shrimp V. harveyi.
PENGARUH PERENDAMAN EKSTRAK BUAH BELIMBING WULUH (Averrhoa bilimbi L.) UNTUK MENGOBATI INFEKSI Aeromonas hydrophila PADA IKAN MAS (Cyprinus carpio) Khaerani, Laela Rizki; Prayitno, Slamet Budi; Haditomo, A.H. Condro
Journal of Aquaculture Management and Technology Volume 7, Nomor 1, Tahun 2018
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Belimbing wuluh merupakan salah satu tanaman buah asli Indonesia dan daratan Malaya. Buah belimbing wuluh berbentuk elips hingga seperti torpedo dengan panjang 4-10 cm. Buahnya mengandung senyawa flavonoid dan triterpenoid. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman ekstrak buah belimbing wuluh (A. bilimbi L.) terhadap kelulushidupan ikan mas (C. carpio) yang diinfeksi bakteri A. hydrophila. Ikan uji yang digunakan adalah ikan mas (C. carpio) dengan bobot individu rata-rata 12,76±1,62 g/ekor. Ikan dipelihara selama 14 hari dengan padat tebar 1 ekor/L. Penelitian ini dilakukan dengan metode eksperimental menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 kali pengulangan. Perlakuan A, B, C, dan D dengan konsentrasi masing-masing 0 ml/L, 3000 ml/L, 6000 ml/L, 9000 ml/L. Data yang diamati meliputi gejala klinis, kelulushidupan, dan kualitas air. Hasil penelitian menunjukan bahwa hari ke-1 setelah penyuntikan bakteri A. hydrophila, ikan mas menunjukkan gejala klinis berenang pasif, borok, haemorhage, bernafas tidak teratur, warna tubuh memudar, luka dibekas suntikan, sirip gripis. Hasil tersebut memperlihatkan bahwa perlakuan B, C dan D rata-rata memperlihatkan proses penyembuhan mulai dari hari ke-5 pasca perendaman. Kenaikan persentase kelulushidupan tertinggi pada perendaman ikan mas yang diinfeksi A. hydrophila menggunakan ekstrak buah belimbing wuluh ditunjukkan oleh perlakuan C sebesar 70%. Perlakuan B dan D juga memperlihatkan kenaikan persentase kelulushidupan yaitu sebesar 53,33% dan 66,67%. Hasil pengukuran kualitas air selama penelitian berada dalam kisaran yang sesuai untuk kehidupan ikan mas (C. carpio). Kesimpulan yang diperoleh yaitu ekstrak buah belimbing wuluh dapat menyembuhkan infeksi bakteri A. hydrophila pada ikan mas. Star fruit is one of originally from Indonesia and Malaya lands. The shape of this fruit is ellipse such as torpedo with length up to 4-10 cm. The fruit contains flavonoid and triterpenoid. This research aimed to observe the effect of submersion of Averrhoa bilimbi on the survival of common carp (C. carpio) intra muscularly by bacteria A. hydrophila. The test fish used in this research were common carp (Cyprinus  carpio) with average individual weight 12,76±1,62 g/head. The fish were looked after reased 14 days with stocking density 1 individual/L. This research was carried out using Completely Randomized Design (CRD) with 4 treatments and 3 times repetition. Treatment A, B, C, and D were star fruit  extractsat concentration of 0 ml/L, 3000 ml/L, 6000 ml/L, 9000 ml/L respectively. The variable observed in this research consisted of clinical symptom, survival, and water quality. The result of the research shows that day 1 after the injection of bacteria A. hydrophila, common carp showed clinical symptoms passive swim, ulcer, haemorhage, Breathing irregularly , the color of the body faded , a wound in the former an injection, and slightly damaged fin. The result shows that treatment B, C and D commonly shows healing process starting from day 5 after submersion. The highest in survival rate of common carp post infected by A. hydrophila than submensed in extract of star fruit shown by treatment C, B, and D at 70%, 53,33%, and 66,67% respectively. The water quality during the research was appropriate for common carp life (C. carpio). The conclusion was that extract of Averrhoa bilimbi can heal infection of bacteria A. hydrophila in common carp. 
AGENSIA PENYEBAB VIBRIOSIS IKAN LELE DUMBO (Clarias gariepinus) PADA KOLAM BEKAS TAMBAK UDANG Indrarini, Dani; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Tingginya permintaan pasar terhadap ikan lele menyebabkan pembudidaya bekerja keras untuk meningkatkan hasil produksi melalui upaya budidaya intensif dalam pemanfaatan lahan bekas tambak. Seiring dengan adanya pemanfaatan lahan bekas tambak yang memiliki kandungan salinitas rendah, maka dimungkinkan untuk terdeteksinya bakteri genus Vibrio. Vibriosis merupakan penyakit bakterial yang sangat merugikan usaha budidaya ikan karena dalam waktu yang sangat singkat dapat menimbulkan tingkat kematian yang tinggi. Metode yang digunakan adalah metode eksploratif. Ikan sampel diambil dari Desa Bulumanis Kabupaten Pati dan Desa Desa Wonosari Kabupaten Demak sebanyak 10 ekor yang diduga terserang penyakit bakteri. Isolasi bakteri menggunakan media TCBS. Organ yang diisolasi yaitu luka – luka pada permukaan tubuh, hati, dan ginjal ikan lele. Hasil isolasi diperoleh 23 isolat lalu diseleksi berdasarkan morfologi koloni hingga diperoleh 5 isolat (LPL14, LDL8, LPG10, LPL4, dan LDH1) untuk uji postulat koch. Hasil penelitian menunjukkan bahwa gejala klinis ikan lele yang terserang Vibriosis adalah luka kemerahan/borok (ulcer) pada permukaan tubuh, hemoragi (luka kemerahan), perut berisi cairan kuning dan sirip gripis yang disertai luka kemerahan. Identifikasi bakteri dilanjutkan dengan uji biokimia. Agensia penyebab Vibriosis ikan lele pada kolam bersalinitas rendah adalah bakteri genus Vibrio  (LPL 14, LDL 8, dan LPG 10), Vibrio vulnificus (LDH 1), dan Vibrio harveyi (LPL4). Pengamatan histopatologi diperoleh bahwa terjadi kerusakan pada organ hati berupa kelainan nekrosis, degenerasi vakuola, melanomakrofag, dan kongesti. High market demand of catfish causes an increase of the farmer effort to increase the production by extensification using unproductive brackish pond area. In a row of using a brackish pond area which has a low salinity, consequently Vibriosis are detected in the pond culture. Vibriosis is a bacterial diseases that can causes loss in aquaculture at a short time can lead a high mortality rate. Research method used explorative method. 10 samples of fish were taken from Bulumanis village, Pati regency and Wonosari village, Demak regencies which were potentially infected by Vibrio. Isolation of bacteria were done in TCBS medium. Bacterial isolates were collected from fish lesion on the body surface, liver, and kidney of catfish. Isolation were able to gained 23 isolates and then 5 isolates (LPL14, LDL8, LPG10, LPL4, and LDH1) were selected based on colony morphology to do postulates koch’s test. The results of this research showed that the clinical signs of catfish infected by Vibrio were redness lesions/ulcer on the body surface, hemorrhagic, fluid inside stomach, and fin eroded with redness wound. Bacterial identification through biochemical test revealed that the causative agent of catfish disease at brackish pond area were bacteria of the genus Vibrio (LPL 14, LDL 8, and LPG 10), Vibrio vulnificus (LDH 1), and Vibrio harveyi (LPL4). Observation of histopathology found necrosis, vacuolar degeneration, melanomacrofage, and congestion in the liver.
PERENDAMAN BERBAGAI DOSIS EKSTRAK DAUN BAKAU (Rhizophora apiculata) UNTUK PENGOBATAN KEPITING BAKAU (Scylla serrata) YANG DIINFEKSI BAKTERI Vibrio harveyi Putri, Amanda Mega; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Budidaya kepiting bakau (Scylla serrata) sering terkendala karena serangan penyakit bakterial Vibrio harveyi. Penggunaan antibiotik dalam jangka panjang dapat menimbulkan resistensi dan mencemari lingkungan. Penggunaan bahan alami untuk pengobatan infeksi bakteri sedang digalakkan, salah satunya adalah ekstrak daun bakau (Rhizophora apiculata). Penelitian ini bertujuan untuk mengetahui pengaruh perendaman ekstrak daun bakau terhadap kelulushidupan, laju pertumbuhan spesifik, dan histopatologi hepatopankreas kepiting serta mengetahui dosis terbaik untuk mengobati kepiting yang diinfeksi bakteri V. harveyi. Kepiting bakau yang digunakan berjumlah 48 ekor dengan berat tubuh antara 40.11±2.53 gram, yang kemudian disuntik bakteri V. harveyi dengan kepadatan 106 CFU/ml secara intramuskular. Perendaman ekstrak daun bakau dilakukan 60 – 90 menit pasca penyuntikan setelah muncul gejala klinis seperti merenggangnya kaki renang dan kaki jalan, karapas menghitam, terdapat bintik putih, dan munculnya bercak merah. Perendaman ekstrak daun bakau berpengaruh sangat nyata (P<0.05) dan P(<0.01) terhadap kelulushidupan kepiting yang diinfeksi bakteri V. harveyi. Nilai rerata kelulushidupan tertinggi hingga terendah berturut-turut yaitu 100% (perlakuan D), 66.67% (perlakuan C), 58.33% (perlakuan B), dan 41.67% (perlakuan A). Sebaliknya, perendaman ekstrak daun bakau tidak memberikan pengaruh nyata (P>0.05) dan P(>0.01) terhadap pertumbuhan kepiting bakau. Hasil pengamatan histopatologi diperoleh adanya kerusakan berupa nekrosis dan vakuolisasi pada organ hepatopankreas. Pada penelitian ini, dosis 900 ppm merupakan dosis terbaik dalam mengobati kepiting bakau yang diinfeksi V. harveyi yang mampu memberikan efek penyembuhan terhadap kepiting bakau. Mud crab (Scylla serrata) culture often found obstacle due to bacterial infection Vibrio harveyi. Long-term use of antibiotics cause bacterial resistante and contaminated the environment. Natural ingredients has been for treatment of diseased crab, one of which was mangrove leaf extract (Rhizophora apiculata). This research was aimed to observe the effect of immersion mangrove leaf extract to the survival rate, specific growth rate, and histopathological picture of hepatopancreas mud crab and to know the best dose to treat mud crab that was infected by bacteria V. harveyi. Mud crab used in this research was 48 animals  with body weight between 40.11±2.53 grams, then injected by bacteria V. harveyi with a density of 106 CFU/ml intramuscularly. Dipping in mangrove leaf extract was conducted for 60 – 90 minutes after injection with the appearance of clinical signs such as wide apart swimming and walking legs, blackened carapace, white spots, and red spots. Dipping of mangrove leaf extract significantly effect (P<0.05) and P(<0.01) on the survival of mud crab that was infected by bacteria V. harveyi. The average value of highest to the lowest survival 100% (treatment D), 66.67% (treatment C), 58.33% (treatment B), and 41.67% (treatment A) respectively. The other way, the submersion with mangrove leaf extract showed not giving significant different (P>0.05) and P(>0.01) on growth of mud crab. Histopathological observations obtained were necrosis and vakuolisation in hepatopancreas organ. In this research, dose of 900 ppm give the best treatment to mud crab (S. serrata) were infected V. harveyi. Therefore, immersion mangrove (R. apiculata) leaf extract was able to provides a healing effect on mud crab infected bacteria V. harveyi.
PENGGUNAAN EKSTRAK JAHE MERAH (Zingiber officinale var. Rubrum) SEBAGAI ALTERNATIF PENGOBATAN IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI BAKTERI Aeromonas hydrophila Indriani, Aprilia Dwi; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Ikan nila (Oreochromis niloticus) merupakan jenis ikan air tawar yang mudah dipelihara. Intensifikasi budidaya dapat menyebabkan serangan penyakit bakteri seperti Aeromonas hydrophila. Hal ini dapat menyebabkan kerugian ekonomi dalam kegiatan budidaya. Upaya yang dilakukan untuk mengobati serangan bakteri A. hydrophila salah satunya menggunakan bahan alami, ekstrak jahe merah (Zingiber officinale var. Rubrum). Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan ekstrak jahe merah untuk mengobati ikan nila yang diinfeksi  A. hydrophila. Ikan nila yang digunakan sebanyak 120 ekor dengan ukuran 7 – 9 cm kemudian disuntik bakteri A. hydrophila dengan kepadatan 108 CFU/ml secara intramuskular. Pelarut yang digunakan dalam ekstraksi jahe merah adalah ethanol 96%. Pengobatan ini menggunakan metode short bathing selama 8 menit dan dilakukan sebanyak 2 kali selama penelitian, yaitu hari pertama dan hari ke 8 setelah penyuntikan. Penggunaan ektrak jahe merah berpengaruh nyata (P<0,05) untuk mengobati ikan nila yang diinfeksi A. hydrophila dan ekstrak ini mampu menyembuhkan luka (ulcer) pada ikan nila yang diinfeksi A. hydrophila dalam 14 hari. Nilai rata-rata kelulushisupan tertinggi hingga terendah berturut-turut yaitu 57,78% (perlakuan C), 46,71% (perlakuan B), 40,78% (perlakuan D) dan 8,85% (perlakuan A). Tilapia (O. niloticus)  is one of the freshwater species which is easy to be cultured. Intensification of Tilapia causes bacterial diseases, such as A. hydrophila. This case resulted in economic disadvantage in aquaculture. One of the effort used to eliminate A. hydrophila infection was the usage of natural ingredient, red ginger (Zingiber officinale var. Rubrum) extract. The aims of this research was to determine the effect of red ginger extract for healing Tilapia infected by A. hydrophila. The Tilapia used were 120 fish, around 7 – 9 cm in size and were injected by bacteria A. hydrophila with density 108CFU / mL intramuscularly. Red ginger extract was obtained by dipping in ethanol 96%. This treatment used short bathing method for 8 minutes and it was done as much as 2 times, at the 1st and 8th days post-injection. The utilization of red ginger extract showed a significant effect (P<0.05) for healing Tilapia infected by A. hydrophila and this extract could heal ulcer on Tilapia infected by A. hydrophila in 14 days. The results showed that the average value of the highest to the lowest survival in a row were 57.78% (treatment C), 46.71% (treatment B), 40.78% (treatment D) and 8.85% (treatment A) respectively.
PATOGENISITAS ISOLAT K14 YANG DIISOLASI DARI LELE DUMBO (Clarias gariepinus) YANG BERASAL DARI DEMAK Wulandari, Ayu; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Vol 3. No 2 (2014): Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Budidaya lele (Clarias gariepinus) berkembang pesat dan memiliki nilai ekonomis yang tinggi. Ikan lele dapat dibudidayakan di lahan dan sumber air tebatas serta padat penebaran tinggi.  Pertumbuhan ikan lele cepat dan pemasaran mudah. Penelitian ini bertujuan untuk mengetahui tingkat patogenisitas dan leukosit total ikan lele dumbo (C. gariepinus) yang telah diinfeksi Isolat bakteri K14 dengan kepadatan yang berbeda serta identifikasi Isolat bakteri K14 pada lele dumbo secara biomolekuler berbasis PCR 16S rDNA. Penelitian ini menggunakan metode eksperimental. Ikan uji yang digunakan adalah ikan lele dumbo sebanyak 210 ekor dengan ukuran 8.02 ± 0.6 cm Kepadatan Isolat bakteri K14 yang digunakan dalam penelitian adalah perlakuan (A) 100 CFU/mL, perlakuan (B) 104 CFU/ml, perlakuan (C) 105 CFU/ml, perlakuan (D) 106 CFU/ml, perlakuan (E) 107 CFU/ml, perlakuan (F) 108 CFU/ml, dan perlakuan (G) 109 CFU/ml. Pengamatan gejala klinis dilakukan selama 96 jam dan pengamatan leukosit total dilakukan setiap 24 jam sekali selama 6 hari. Hasil penelusuran sequen 16S rDNA mengunakan internet melalui sistem BLAST. Hasil pengamatan gejala klinis lele dumbo yang diinfeksi Isolat bakteri K14 adalah ikan berenang lemah di dasar air, berenang vertikal, nafsu makan berkurang, kulit mengelupas, haemorragic pada tubuh, kemudian timbulnya radang kemudian menjadi luka yang terbuka (ulcer). Kematian ikan tertinggi terlihat pada perlakuan F (108 CFU/mL) dan G (109 CFU/mL) yaitu 30 ekor, kemudian perlakuan E (107 CFU/mL) sebesar 24 ekor, perlakuan D (106 CFU/mL) sebesar 23 ekor, perlakuan C (105 CFU/mL) sebesar 22 ekor, dan kematian ikan paling sedikit dialami pada perlakuan B (104 CFU/mL) sebesar 2 ekor. Nilai LD50 dalam uji patogenisitas menunjukkan bahwa konsentrasi bakteri yang dapat mematikan 50% populasi ikan lele dumbo dalam waktu 96 jam adalah 4.977x105 CFU/ml. Jumlah leukosit total tertinggi setelah 24 jam penyuntikan Isolat bakteri K14 pada perlakuan G adalah 3.41x104 sel/mm3 dan terendah pada perlakuan A sebanyak 1.57x104 sel/mm3. Hasil penelusuran berdasarkan 16S rDNA menggunakan sistem BLAST diperoleh bahwa homogenitas sebesar 96% terhadap Aeromonas sp. Catfish farming have high value and high demand because a catfish can growt in limited water resources with high density. Catfish growth fast and easy to sell them. The purpose of this research was to determine the level of pathogenicity of K14 isolate to catfish (C. gariepinus that have inframoscularly nipelia misl K14 isolateat different densities and then biomolecularly identified based on PCR 16S Rdna. This research used experimental method. The fish sample was used catfish with 8.02 ± 0.6 cm leght. The density of bacteria used in this research were (A) PBS, (B) 104 CFU/mL, (C) 105 CFU/mL, (D) 106 CFU/mL, (E) 107 CFU/mL, (F) 108 CFU/mL, (G) 109 CFU/mL. The observation of clinical signs carried out for 96 hours and total leukocytes were observed every 24 hours for 6 days. 16S rDNA  sequences of K14 isolate was casuistry the BLAST system. The clinical sign of catfish that infected by K14 isolates obtained that fish showed swimming weakly, found till vertically, poor appetite, skin peeling, haemoragic then followed by the inflammation and ulcer. The highest fish mortality was seen in the treatments F (108 CFU/mL) and G(109 CFU/mL) by 30 animals, E (107 CFU/mL) treatments 24 animal, D treatment  106 CFU/mL was valued 23 animals, C treatment 105 CFU/mL was 22 animals and B treatment 104 CFU/mL was 2 animals respectively. LD50 value of K14 demonstrated that the concentration of 105 CFU/mL has able to kill 50% of treated catfish within 96 hours. The highest total leukocytes after 24 hours injection of K14 isolate was found in the G treatment valued 3.41 x 104 cells/mm3 and the lowest found in A treatment at 1.57 x 104 cells/mm3. Reidentification of K 14 isolate using moleculer approach 16S rDNA then presented the sequence againt existing gen bank by BLAST system indicated that genetically the K14 isolate closes 96% to Aeromonas sp.
PENGARUH KONSENTRASI KONSORSIUM BAKTERI K1, K2 DAN K3 TERHADAP STATUS KESEHATAN RUMPUT LAUT (Eucheuma cottonii) Siregar, Marwenni; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 5, Nomor 1, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Eucheuma cottonii berperan sebagai penyumbang utama produksi perikanan. Faktor penghambat produksi adalah penyakit ice-ice. Ice-ice disebabkan oleh lingkungan yang tidak sesuai, rumput laut akan stres sehingga melepaskan senyawa haloamin menimbulkan lendir dan memicu tumbuhnya bakteri. Penelitian ini bertujuan untuk mengetahui pengaruh konsorsium bakteri K1, K2 dan K3 dengan konsentrasi yang berbeda terhadap kesehatan E cottonii. Metode pada penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuannya dengan konsorsium bakteri K1,K2 dan K3 yang sama dan konsentrasi yang berbeda yaitu A (tanpa inokulasi/kontrol), B (5x106 CFU/mL), C (2.3x107 CFU/mL, dan D (2.2x108 CFU/mL). E. cottonii bobot 1 gram dan panjang 5 cm dipelihara dalam botol kaca yang diisi air laut steril 200 ml selama 9 hari. Kondisi suhu 28°C, salinitas 30 ‰, dan pH 8. Pemeliharaan menggunakan shaker kecepatan 100 rpm dan pemaparan cahaya 12 jam terang 12 jam gelap. Hasil penelitian menunjukkan konsentrasi konsorsium K1,K2 dan K3 yang berbeda berpengaruh nyata (P < 0,01) terhadap nilai pertumbuhan mutlak E. cottonii. Gejala klinis yang ditimbulkan adalah terdapat spot putih pada thallus, warna thallus memudar, cabang melepuh dan putus. Bakteri konsorsium yang menyebabkan gejala klinis tersebut adalah Corynebacterium, Vibrio hollisae dan Pseudomonas sp. Berdasarkan hasil penelitian disimpulkan bahwa konsorsium bakteri K1, K2 dan K3 dengan konsentrasi 2.2x108 CFU/mL dapat menyebabkan penurunan bobot dan gejala klinis tertinggi yang ditimbulkan. Eucheuma cottonii role as a major contributor to the production of fisheries. Factors inhibiting the production is ice-ice disease. Ice-ice caused by the environment are not incompatible, seaweed would stress that cause mucus haloamin releasing compounds and trigger the growth of bacteria. This study aims to determine the effect of bacterial consortium K1, K2 and K3 with different concentrations on the health of E. cottonii. The method in this study using a completely randomized design (CRD) with 4 treatments and 3 replications. Treatment with bacterial consortium K1, K2 and K3 are the same and different concentrations namely A (without inoculation / control), B (5x106 CFU / ml), C (2.3x107 CFU / ml, and D (2.2x108 CFU / ml ). E. cottonii weighs 1 gram and 5 cm long preserved in a glass jar filled with 200 ml of sterile sea water for 9 days. Conditions 28 ° C, salinity 30 ‰, and pH 8. Maintenance using a shaker speed of 100 rpm and the exposure light 12 hours of light to 12 hours dark. The results showed concentrations of a consortium of K1, K2 and K3 are different significant (P <0.01) to the value of absolute growth E. cottonii. The clinical symptoms are caused there are white spots on thallus, thallus color fading, branch blister and break up. Bacteria that cause clinical symptoms consortium is Corynebacterium, Vibrio hollisae and Pseudomonas sp. Based on the results of the study concluded that the bacterial consortium K1, K2 and K3 with a concentration of 2.2x108 CFU / ml can cause weight loss and highest clinical symptoms caused.
Identifikasi Dan Uji Postulat Koch Agensia Penyebab Penyakit Bakteri Pada Ikan Lele (Clarias gariepinus) yang Berasal dari Demak Wijayanti, Aulia Resty; -, Sarjito; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Vol 2, No 2 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

The aim of the research was to find out the clinical sign of the catfish sample that has been infected by the bacteria and to find out the causative agent bacteria diseases of Clarias gariepinus from Demak. The fish sample collection used purposive random sampling method. The observation of clinical sign was implemented toward ten catfish samples. The isolation of bacteria from moribund fish surface and kidney of catfish was conducted by streaking method on NA and GSP media. Based on the characteristic of morphology (colour, form of colony), it was obtained five isolate from eleven isolates of bacteria for the next test. The test of postulat koch toward five isolates of ten test fish and characterized them to causative agent bacterial through morphological and biochemical characterization. The result of the research showed that the clinical sign were detected irregular swimming, balance disorders, fish stand still at the bottom of the aquarium, the fish limp, injury/red blisters at the injection site, a pale body color, porous on tails, fins and antennas. The test results of postulates koch obtained that the five isolates (D7, D10, D12, D14, D16) were capable of causing pain on test fish. Based on the postulate test also showed only two isolates (D7 and D12) were caused mortality of 30% of the test fish. The results of the morphological and biochemical characterization found that the five agents causing disease in fish from Demak were abbreviated namely of Pseudomonas plecoglossicida, Flavobacterium sp., Edwardsiella ictaluri, Aeromonas caviae and Aeromonas caviae, respectively.
STUDI KASUS INFESTASI Cichlidogyrus PADA IKAN NILA (Oreochromis niloticus) DARI SATKER BPBIAT DAN LUAR SATKER BPBIAT JANTI, KLATEN, JAWA TENGAH Kamil, Mia Rahmah; Prayitno, Slamet Budi; Desrina, - -
Journal of Aquaculture Management and Technology Volume 6, Nomor 4, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Cichlidogyrus merupakan salah satu parasit dari golongan monogenea yang meninfestasi ikan nila. Cichlidogyrus menyerang pada insang yang menempel pada filamen insang dan memakan sel-sel epitel insang, mukus dan darah pada insang. Hal ini dapat menimbulkan kematian bagi ikan-ikan nila yang masih muda atau dalam keadaan lemah akibat stres dan infeksi akut. Penelitian ini bertujuan untuk mengetahui gejala klinis ikan yang terinfestasi Cichlidogyrus, morfologi dari Cichlidogyrus, jumlah, prevalensi, intensitas Cichlidogyrus serta uji statistika perbandingan antara jumlah Cichlidogyrus, prevalensi dan intensitas antara sampel Satker BPBIAT Janti dan Masyarakat Desa Janti. Metode yang digunakan yakni monitoring menggunakan data primer dan data sekunder. Materi yang digunakan yaitu ikan nila sebanyak 252 ekor di ambil secara acak dari kolam Satker BPBIAT dan kolam Masyarakat Desa Janti. Pengamatan ektoparasit dilakukan dengan pembuatan preparat segar dan selanjutnya diamati dibawah mikroskop. Hasil dari penelitian ini yakni gejala klinis yang tampak pada ikan nila yakni berupa produksi lendir berlebih pada insang dan warna insang merah pucat. Cichlidogyrus mempunyai tubuh pipih memanjang, memiliki sepasang mata, faring yang berbentuk bulat, cuping kepala atau cephalic lobe, organ reproduksi, haptor yang terdiri dari 2 pasang jangkar dan 7 pasang kait. Jumlah Cichlidogyrus pada kolam Satker BPBIAT Janti 1169 individu dan 641 individu pada Kolam Masyarakat Desa Janti. Prevalensi Cichlidogyrus pada Satker BPBIAT Janti 65,87% dan kolam Masyarakat Desa Janti 69,04%. Intensitas rata-rata Cichlidogyrus sebesar 12 ind/ekor pada kolam Satker BPBIAT Janti  dan 6 ind/ekor pada kolam Masyarakat Desa Janti. Uji t independen perbandingan jumlah Cichlidogyrus, prevalensi dan intensitas tidak berbeda nyata antara sampel Satker BPBIAT Janti dan Masyarakat Desa Janti. Dapat disimpulkan bahwa dua lokasi tidak berbeda signifikan terhadap jumlah Cichlidogyrus, prevalensi dan intensitas. Cichlidogyrus is one of the monogenean parasites that infestated tilapia. Cichlidogyrus anchored the gill filaments and fed on the epithelial cells, mucus and blood of the gills. This could lead to death for young tilapia or weaken due to stress and acute infection. The aim of this research were to observe the clinical symptoms of Cichlidogyrus infected fish, Cichlidogyrus morphology, infestation, prevalence, and intensity between Satker BPBIAT Janti and Janti Village Community were compared. Monitoring of the both pond sites and interviewed were carried out to abtain primary and secondary data. The material used were 252 tilapia fish taken random by from Satker BPBIAT ponds and Janti Village Community Ponds. Ectoparasite observations were performed by fresh smears  and subsequently observed under a microscope. The results showed that  clinical symptoms appeared in the tilapia were excess of mucus production in the gills and pale gill color. Cichlidogyrus has elongated flat body, a pair of eyes, a round pharynx, a head lobe or a cephalic lobe, a reproductive organ, a haptor consisting of 2 pairs of anchors and 7 pairs of hooks. Number of Cichlidogyrus in Satker BPBIAT Janti were 1169 individuals while in Janti Village Community Ponds were 641 individuals. Prevalence of Cichlidogyrus on Satker BPBIAT Janti was 65,87% and Janti Village Community Pool was 69,04%. Intensity of Cichlidogyrus amounted to 12 ind / tail and 6 ind / tail on Satker BPBIAT Janti and Janti Village Community Ponds respectively. The independent t-test of Cichlidogyrus comparison, in prevalence and intensity have no significant differences. It can be concluded that those two location has no significant difference on Cichlidogyrus infestation, prevalence and intensity.
Gambaran Parameter Hematologis Pada Ikan Nila (Oreochromis niloticus) Yang Diberi Vaksin DNA Streptococcus iniae Dengan Dosis Yang Berbeda Utami, Devitha Tri; Prayitno, Slamet Budi; Hastuti, Sri; Santika, Ayi
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Tilapia (O. niloticus) is one of freshwater species that has rapid growth and easily been cultivated. One of the constraints in cultivating Tilapia is Streptococciasis disease caused by Streptococcus iniae. The purpose of this research was to determine the haematological response of Tilapia that was given by DNA vaccine of S. iniae and challenged with S. iniae, and to determine the most effective dose of DNA vaccine based on haematological performances in the prevention of bacteria S. iniae. The fish samples used were Tilapia with length of 7,79 cm ± 0.48 as many as 150 animals or 10 animals per treatment. Five (5) treatments and three (3) replications were administered in this research, there were A (0 ŋg/µL), B (10ŋg/µL), C (20ŋg/µL), D (30ŋg/µL), dan E (40ŋg/µL) of DNA vaccines the injected intramuscularly. Fish were maintained for 30 days, then challenged with S. iniae for 14 days. Measurement of haematological performances include haematocrit, total leukocyte, differential leukocytes, and phagocytosis index carried out every 7 days. The observation of clinical signs of fish after challenge by S. iniae obtained that fish in A and B treatment showed clinical signs more quickly than fish in C, D, and E treatment. The results showed that DNA vaccines S. iniae in E treatment significantly different on haematocrit levels (25,67%), total leukocytes (9,46x104 cells/mm3), lymphocytes (80,33%), monocytes (5,67%), neutrophils (14%), phagocytosis index (22,33%), and survival rate (70%). It can be conclude that dosage of DNA vaccine 40ng/μl is the most effective dosage which is immunogenic and protective in raising Tilapia immunity against bacterial S. iniae based on haematological aspects.
Co-Authors - Aminah - Desrina, - - Istikhanah - Susanti, - - Triyaningsih Adhi Kurniawan Agatya Sara Ardiantami, Agatya Sara Agil Setya Utomo, Agil Setya Agung Sudaryono Agus Sabdono Agustina Agustina Agustina Alfabetian Condro Haditomo Alfabetian Harjuna Condro Haditomo Alfabetian Harjuno Condro Haditomo Amanda Mega Putri, Amanda Mega Ambaryanto Ambaryanto Amelia Rimadhani ANGELA MARIANA LUSIASTUTI Anggun Putriani Situmorang, Anggun Putriani Aninditia Sabdaningsih Anisa Dwiaryani Latifah Annisa Amalia Awanis Aprilia Dwi Indriani Ardulanisa, Ridha Arif, Gunarso Arifianto Heraedi Asep Akmal Aonullah Asriyana, . Aulia Ayu Ermantianingrum Aulia Resty Wijayanti Ayi Santika Ayu Wulandari Azis Nur Bambang Bagus Oktori Sutrisno cahyadi, jimmy Chyntia Arindita Dani Indrarini Desrina Desrina Desrina, - - Devitha Tri Utami Dewi Nurhayati Dian Ratna Sari Didik Wahju Hendro Tjahjo Edward Raharja, Edward Endah Setyowati Eni Ashfa Ashofa Fajar Purnama, Muhammad Ferdian Bagus Feriandika Fifiana Zulaekah fikih rosalina pratiwi Frida Purwanti Gina Saptiani Gina Saptiani Haditomo, A.H. Condro Hardyta Noviar Rahma Hariyadi, Putut Hasna, Salma Khoironnida Hendrawan, Andika Luky Setiyo Ida Ayu Putu Sri Widnyani Ikhsan Pratama Indah Febry Hastari Intan Eska Amalia Syahida Johannes Hutabarat Kamil, Mia Rahmah Khaerani, Laela Rizki Kurniawan Kurniawan Kusuma, Winanda Adi Laksono Trisnantoro Lestari Lakhsmi Widowati Linuwih Aluh Prastiti Lukman Anugrah Agung Lusi Dianti Marwenni Siregar, Marwenni Max Rudolf Muskananfola Mohammad Saleh Lubis Monica Nanda Muhammad Fajar Purnama Nailil Muna Nida Qolbi Salma Rochani Nita Amelia Nur Annisa Nuri Nia Yanti, Nuri Nia Nurmanita Rismaningsih Nurul Hidayati Ocky Karna Radjasa Peppy Dewi Fitriyanti Pinandoyo Pinandoyo Pramudita Apriliyanti Pricilia, Shella Pujiono Wahyu Purnomo Pungki Nanda Pratama Purnama, Muhammad Putut Har Riyadi R. Dewi Dharina Nurjannah Restiana Wisnu Ariyati Rismaningsih, Nurmanita Ristiawan Agung Nugroho Rohita Sari Rosa Amalia Rusda, Yusrotul Rusydina Qamarul Salikin Sahala Hutabarat Samsul Rizal Sapto P Putro Sarah, Humidah Sarjito - Sekar Ayu Chairunnisa Seto Windarto Shiddieqi, Hasbi Ash Siti Nurjanah Siti Ziyadaturrohmah Solly Aryza Sri Nuryati Sri Rejeki Subandiyono Subandiyono Subhan Subhan Sulisyaningrum Sulisyaningrum Sumbung, Sepri Suryanti Suryanto, Damang Sutrisno Anggoro Syakuri, Hamdan Teguh Eko Suryo Agil Hermawan Tristiana Yuniarti Triwulandari, Atita Utari, Viola Indah Vivi Endar Herawati Widiana, Giacinta Risti Windarto, Seto Yelliana Fatmawati Suwarno Yohanes Kristiawan Artanto YS Darmanto Yunia Dharmastuti Widyaningsih Yusuf Kamlasi Zairin Zain