Article Per Year (5 Year)
p-Index From 2021 - 2026
3.237
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology International Journal of Science and Engineering (IJSE) Journal of Aquaculture Management and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Jurnal Kelautan Tropis Omni-Akuatika Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Indonesian Fisheries Research Journal Journal of Aquaculture and Fish Health Jurnal Airaha Ilmu Perikanan Tropis Nusantara Jurnal Kedokteran Hewan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Jurnal Perikanan
Slamet Budi Prayitno
Aquaculture Study Program, Department Of Aquaculture, Faculty Of Fisheries And Marine Science, Diponegoro University, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

Published : 87 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 87 Documents
Search

 2   3   4   5   6 
KAJIAN KESESUAIAN LAHAN TAMBAK UDANG VANAME (litopenaeus vannamei) DI KECAMATAN CIJULANG DAN PARIGI, PANGANDARAN, JAWA BARAT DENGAN PENERAPAN APLIKASI SISTEM INFORMASI GEOGRAFIS Kusuma, Winanda Adi; Prayitno, Slamet Budi; Ariyati, Restiana Wisnu
Journal of Aquaculture Management and Technology Volume 6, Nomor 4, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.64 KB)

Abstract

Udang vaname merupakan salah satu produk perikan terbesar yang menjadi komoditas ekspor. Produksi udang vaname pada tahun 2010-2014 mengalami kenaikan rata-rata sebesar 20,49 %. Daerah sekitar muara sungai Kecamatan Cijulang dan Parigi adalah salah satu daerah yang banyak mengembangkan budidaya tambak udang vaname. Namun dalam pengembangannya sering mengalami kegagalan panen. Dengan demikian perlu dilakukan uji untuk mengevaluasi dan menganalisis kesesuaian lahan tambak. Salah satu alternatif yang dapat digunakan adalah Sistem Informasi Geografis (SIG). Tujuan dari penelitian ini adalah menganalisis kesesuaian lahan tambak di Kecamatan Cijulang (Desa Kondangjajar, Desa Sanghyang Kalang, Desa Margacinta) dan Kecamatan Parigi (Desa Karangjaladri), Kabupaten Pangandaran, Provinsi Jawa Barat dan mengetahui pemenuhan kebutuhan air selama masa produksi udang vaname. Metode penelitian yang digunakan adalah metode survei. Metode survei ini dilakukan untuk mendapatkan data primer yang berupa data kualitas perairan, baik parameter fisik maupun kimia. Selain itu, dilakukan kegiatan untuk mengumpulkan data-data sekunder. Data yang diperoleh tersebut kemudian diolah pada citra satelit sehingga dihasilkan suatu model dasar peta tematik, peta dasar tematik yang dihasilkan kemudian digunakan untuk mengevaluasi kelayakan tambak serta mengetahui perubahan yang terjadi, sehingga dapat menduga nilai potensi produksi tambak. Penelitian ini dilakukan pada bulan Maret – April 2017. Hasil penelitian ini menunjukkan bahwa secara umum kondisi perairan tambak udang di Kecamatan Cijulang dan Parigi Secara keseluruhan dapat mendukung usaha budidaya udang vaname. Dari tujuh titik yang diamati tergolong kedalam kategori sesuai (S2) dan kategori sangat sesuai (S3). Ketersediaan pompa untuk pemenuhan kebutuhan air selama produksi di setiap titik penelitian telah terpenuhi ≥ 100%. Vannamei shrimp is one of the biggest fishery export commodity. Its production in 2010-2014 increased around 20,49 %. The area around Cijulang and Parigi subdistrict estuary is one of the area which are developing vannamei aquaculture. However, on its development, harvest failure frequently happened. Therefore, some test should be conducted to evaluate and analyze the pond suitability. One of the alternatives which can be used is Geographical Information System (GIS). The aim of this study was to analyze the pond suitatibility in Cijulang Subdistrict (Kondangjajar, Sanghyang Kalang, Margacinta Village) and Parigi Subdistrict (Karangjaladri Village), Pangandaran District, West Java Province and determine the fulfillment of water needs during vannamei production. The research used survey method. The survey was conducted to obtain primary data in the form of water quality data, both physical and chemistry parameter. Secondary data was also obtained to support the findings. The data obtained then processed on satellite imagery, so the basic model of the thematic map was produced. The result of the basic thematic map then used to evaluate the pond suitatibility as well as determine the changes that occur, also able to analyse the pond’s production potential value. This study was conducted on March-April 2017. This result showed that in general, the pond condition in Cijulang and Parigi subdistrict relatively able to support the vannamei production. From the seven spots observed was classified into suitable category (S2) and very suitable category (S3). The pump availability for the fulfillment of water needs during production on each research spots has been fulfilled.
KARAKTERISASI AGENSIA PENYEBAB VIBRIOSIS DAN GAMBARAN HISTOLOGI IKAN KERAPU MACAN (Epinephelus fuscoguttatus) DARI KARAMBA JARING APUNG TELUK HURUN LAMPUNG Hastari, Indah Febry; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.372 KB)

Abstract

Penyakit bakterial yang menyerang ikan kerapu macan (Epinephelus fuscoguttatus) merupakan salah satu jenis penyakit yang bersifat infeksius. Penelitian ini bertujuan untuk mengkaji Vibrio patogen penyebab vibriosis ikan kerapu macan di Karamba Jaring Apung Teluk Hurun Lampung, serta gambaran histologi ikan kerapu yang terinfeksi vibriosis. Sebanyak 6 isolat Vibrio diisolasi dari bagian limpa, ginjal dan luka kerapu macan yang menunjukkan gejala vibriosis, pada medium Thiosulfat Citrat Bile Salt Agar (TCBSA). Materi yang digunakan yaitu 10 ikan kerapu macan sakit berukuran 12-24 cm yang berasal dari Karamba Jaring Apung Teluk Hurun Lampung. Isolasi bakteri menggunakan media TSA dan TCBS dengan metode streak pada limpa, ginjal dan luka ikan. Bakteri dipanen dan disuntikkan terhadap 8 ekor ikan kerapu bebek (C. altivelis) ukuran 7-9 cm. Karakterisasi isolat dilakukan secara morfologi dan biokomia. Uji histologi dilakukan pada ikan yang terinfeksi bakteri vibrio. Hasil penelitiaan menunjukkan bahwa gejala klinis ikan kerapu macan yang terserang histologi adalah haemorhagik pada pangkal sirip ekor, warna hati pucat, kondisi ikan kerapu lemah. Hasil isolasi didapatkan 17 isolat bakteri. Seleksi berdasarkan morfologi koloni bakteri didapatkan 6 bakteri (IN-03, IN-06, IN-11, IN-12, IN-24 dan IN-22) untuk dilakukan uji postulat koch. Hasil uji postulat koch menunjukkan 6 isolat bakteri bersifat patogen dengan prosentase kematiaan tertinggi terdapat pada isolat IN-12 yaitu 75% dalam waktu kurang dari 78 jam. Agensia penyebab vibriosis pada ikan kerapu macan dari Lampung V. alginolyticus, V. fluvialis, V. metschnikovii, V. Vulnificus dan V. logei. Selain itu gambaran histologi yang terjadi yaitu nekrosis pada hati, hiperflasia serta fusi lamella sekunder pada insang, dan infiltrasi leukosit pada limpa.  Bacterial disease that attacks the tiger grouper (Epinephelus fuscoguttatus) is one of the infections disease. This study aims to assess the pathogenic Vibrio causes vibriosis tiger grouper in floating net Hurun Bay of Lampung and histology identified grouper vibriosis. A total of 6 isolates of Vibrio isolated from the spleen, kidney and tiger grouper wounds that show symptoms of vibriosis, on Thiosulfate Citrate Medium Bile Salt Agar (TCBSA). The materials used were 10 tiger grouper measuring 12-24 cm from Karamba Floating Net Hurun Bay Lampung. Isolation of bacteria using TSA and TCBS media with methods streak in the spleen kidneys and injured fish. Bacteria were harvested and injected the 8 tailed duck grouper (C. altivelis) size 7-9 cm. Characterization of isolates conducted morphological and biochemical. Histology test performed on fish that are infected bacteria vibrio. The result showed that symptoms clinical tiger grouper infected histology is haemorhagic at the base of the tail fin, the colour of liver pale, the condition of grouper weak. The result obtained 17 isolation isolates bacteria. Selection on the basis of morphological bacteria colonies acquired 6 bacteria (IN-03, IN-06, IN-11, IN-12, IN-24 dan IN-22) to the test postulate koch. The result test postulate koch show 6 isolates bacteria are pathogenic by high prosentase deathly of isolate IN-12 is 75% in less than 78 hours. Causative agent of vibriosis on tiger grouper from Lampung by V. alginolyticus, V. fluvialis, V. metschnikovii, V. Vulnificus and V. logei. Besides an image of histology happened necrosis, which is on the liver hiperflasia which secondary fusi lamella from lamella gills and ilfiltration gills and infiltration leukosit on the gills.
ANALISA POTENSI PRODUKSI TAMBAK IKAN BANDENG (Chanos chanos) DI KECAMATAN WEDUNG DENGAN PENERAPAN APLIKASI TEKNOLOGI PENGINDERAAN JAUH Widiana, Giacinta Risti; Prayitno, Slamet Budi; Widowati, Lestari Lakhsmi
Journal of Aquaculture Management and Technology Volume 6, Nomor 4, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.111 KB)

Abstract

Ikan bandeng (Chanos chanos) merupakan salah satu ikan air payau yang banyak diminati di Indonesia untuk dikembangkan. Ikan bandeng di Kecamatan Wedung banyak dibudidayakan dalam tambak dengan sistem ekstensif, yaitu sistem budidaya tanpa pemberian pakan tambahan sehingga seluruh hidupnya bergantung pada ketersediaan pakan alami berupa klekap pada tambak tersebut. Kurangnya informasi mengenai karakteristik perairan dan ketersediaan klekap di sekitar lahan budidaya tambak bandeng di Kecamatan Wedung menyebabkan pemanfaatan lahan yang tidak optimal. Tujuan dari penelitian ini adalah untuk menganalisa kesesuaian lahan budidaya tambak bandeng serta menganalisa potensi produksi tambak bandeng di Kecamatan Wedung berdasarkan ketersediaan klekap sebagai sumber pakan alami ikan bandeng. Metode penelitian yang digunakan adalah studi kasus yang meliputi 2 tahapan, yaitu pengumpulan data dan analisa data. Pengumpulan data dilakukan dengan cara pengukuran langsung ke lapangan sebanyak 7 titik penelitian dan wawancara terhadap petani tambak. Data yang telah didapat kemudian diolah dengan menggunakan ArcGis 10.0. Pengohanan data dilakukan dalam beberapa tahapan yaitu pembuatan peta dasar, pembuatan peta kontur sebaran spasial dari setiap parameter, overlay, layout dan scoring. Data yang dihasilkan berupa model spasial. Analisa kesesuaian perairan dilakukan dengan pembuatan matriks kesesuaian kemudian pembobotan dan penghitungan skor berdasarkan tingkat pengaruh dari setiap parameter terhadap daerah yang berpotensi untuk budidaya bandeng. Selanjutnya dilakukan analisa potensi produksi berdasarkan ketersediaan klekap. Hasil yang diperoleh dari penelitian ini adalah luasan tambak Kecamatan Wedung sebesar ± 1.663 ha termasuk dalam kategori cukup sesuai (S2) dan tambak dengan luas ± 1.911 ha masuk dalam kategori sesuai bersyarat (S3). Dilihat dari faktor ketersediaan klekap pada titik I memiliki potensi bandeng 663 kg, titik IV sebanyak 990 kg dan titik VII sebanyak 955 kg. Pertambakan di kecamatan Wedung memiliki potensi untuk dikembangkan karena memiliki total ketersediaan klekap sebesar 120% dari total kebutuhan bandeng.    Milkfish(Chanos chanos) is the one of fishes of brackish water much demanded in Indonesia to be developed. Milkfish in Wedung subdistrict lot of cultivated in ponds with extensively scale, is the system cultivication without additional feed so the entire life  relied on the availability natural feed of klekap on that ponds. Lacking of information regarding the characteristic of the water and availability of klekap around the ponds of milkfish in Wedung subdistrict as led to the utilization of locations became unoptimum. The study was aimed to analysis the suitability of cultivating lands ponds of milkfish and analysis of milkfish ponds potention production in Wedung subdistrict based on availability of klekap as natural feed. The method used is data collection by survey in the field and then analyzed. The data had been collected was done by direct measurement in the field and interviews with fish farmers. The data have been obtained were processed by using ArcGIS 10.0. The steps started with  with base map creation stages, Making contour map of the spatial distribution of each parameter, Overlay, Layout and Scoring which earned spatial model. The analysis was made by making the waters suitability matrix, then weighting and calculating the score based on the degree of each parameter influenced towards the potential areas for cultivation of milkfish. Further analysis based on availability klekap production potential. The results obtained fromt his study is about ± 1.663 ha ponds area in Wedung subdistrict belong to S2 category and ponds with an area ± 1.911 ha belongs to S3 category. Seen from the availability of klekap in station 1 has potential production of bandeng about 663 kg, station IV about 990 kg and station VII about 955 kg. Ponds area in Wedung subdistrict hasthe potential to be developed having total klekap availability about 120% of totalneeds ofmilkfish.
PENGGUNAAN EKSTRAK DAUN BAKAU (Rhizopora apiculata) UNTUK PENGOBATAN KEPITING BAKAU (Scylla serrata) YANG DIINFEKSI BAKTERI Vibrio harveyi TERHADAP KELULUSHIDUPAN Susanti, -; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 5, Nomor 2, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.829 KB)

Abstract

Penyakit bakterial yang sering menyerang kepiting bakau adalah vibriosis. Vibriosis merupakan jenis penyakit penyebab kematian masal pada kepiting. Penyakit bakterial ini menyerang di semua stadia kepiting, baik juvenile hingga kepiting dewasa.  Tujuan dari penelitian ini adalah mengetahui kemampuan ekstrak kasar daun bakau R. apiculata untuk menghambat pertumbuhan V. harveyi secara in vitro dan mengetahui dosis terbaik dari ekstrak daun bakau (R. apiculata) untuk pengobatan kepiting bakau (S. serrata) yang diinfeksi V. harveyi melalui metode perendaman.  Penelitian ini menggunakan metode eksperimen dengan 4 perlakuan dan 3 ulangan, menggunakan 48 ekor kepiting bakau dengan berat rata-rata 60.75±5.17 gram, kemudian di infeksi menggunakan bakteri V. harveyi sebanyak 0,1 ml dengan kepadatan 106 CFU/ml, pada pangkal kaki renang. Setelah timbul gejala klinis, dilakukan perendaman menggunakan ekstrak daun bakau (R. apiculata) selama 1 hari (long bath) dengan konsentarasi 0 ppm, 20.000 ppm, 40.000 ppm dan 60.000 ppm. Hasil penelitian diperoleh bahwa perendaman dengan ekstrak daun bakau (R. apiculata), berpengaruh nyata terhadap kelulushidupan kepiting bakau (S. serrata). Masing-masing nilai kelulushidupan pada perlakuan A, B, C, dan D adalah 58.33%, 91.67%, 83.33% dan 100%. kelulushidupan tertinggi didapatkan pada perlakuan D yaitu 100%, namun konsentrasi terbaik untuk pengobatan kepiting bakau yang terinfeksi V. harveyi adalah perlakuan B dengan dosis 20.000. The bacterial desease that often strikes mud crabs is vibriosis. Vibriosis caused mass mortality of crabs. This bacteriall infected crab in all stages, started from juvenile until adult stages. The purpose of this research was to study the ability of mangrove leaves extracts (R. apiculata) to inhibit the growth  of V. harveyi in vitro and to find the best dosage to treat mud crabs (S. serata) injected by V. harveyi with dipping method. 48 mud crabs with the average weight of 60.75±5.17 gram were used in this research. The experimental crabs were infected by bacterium (V. harveyi) 0,1 ml at density 106 CFU/ml at the base of swimming leg. After showed clinical symptoms, crabs were inmmersed in mangrove leaves extract (R. apiculata) for one day (long bath) at concentration of 0 ppm, 20.000 ppm, 40.000 ppm and 60.000 ppm respectively. The result showed that dipping of mangrove leaves extracts (R. apiculata) significantly affected the survival rate of  mud crab (S. serata). Survival rate of treatment A, B, C and D were 58,33%, 91,67%, 83,33% and 100% respectively. The highest survival rate was performed  by treatment D that was 100%, however the best concentration for the treatment of mud crabs which infected by V. harveyi was treatment B with dose of 20.000 ppm.
PATOGENISITAS Aeromonas hydrophila YANG DIISOLASI DARI LELE DUMBO (Clarias gariepinus) YANG BERASAL DARI BOYOLALI Triyaningsih, -; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Vol 3. No 2 (2014): Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.127 KB)

Abstract

Ikan lele dumbo (Clarias gariepinus) adalah ikan yang cukup populer di kalangan masyarakat Indonesia, khususnya di pulau Jawa. Salah satu kendala dalam budidaya lele dumbo adalah serangan penyakit MAS (Motile Aeromonas Septicemiae) yang disebabkan oleh bakteri A. hydrophila. Penelitian ini bertujuan untuk mengetahui patogenisitas bakteri A. hydrophila dan pengaruhnya terhadap total leukosit lele dumbo. Penelitian ini dilakukan di Laboratorium Terpadu dan Laboratorium Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro pada bulan Mei - September 2013. Ikan uji yang digunakan adalah 180 ekor lele dumbo sehat ukuran 7 - 9 cm. Penelitian ini menggunakan metode eksperimen. Konsentrasi bakteri yang digunakan adalah A, B, C, D, E, dan F berturut- turut 100 CFU/ml, 105 CFU/ml ¸106 CFU/ml 107 CFU/ml, 108 CFU/ml dan 109 CFU/ml Pengamatan gejala klinis dilakukan tiap 6 jam sekali selama 96 jam dan pengamatan total leukosit dilakukan tiap 24 jam sekali selama 6 hari. Hasil penelitian menunjukan gejala klinis lele dumbo yang diinfeksi A. hydrophila adalah penurunan respon terhadap pakan, berenang abnormal, luka dibagian tubuh. Berdasarkan perhitungan uji LD50 didapatkan dosis yang dapat mematikan 50% ikan uji dalam waktu 96 jam adalah bakteri A.hydrophila dengan konsentrasi 1,25x106 cfu/ml, untuk perhitungan rata-rata total leukosit tertinggi pada (F) 3,69x104 sel/mm3 yaitu sebesar dan terendah pada perlakuan (A) yaitu sebesar 1,86x104 sel/mm3. The Catfish (Clarias gariepinus) is popular fish among the people of Indonesia, especially in Java. One problems  in catfish culture is a disease from MAS (Motile Aeromonas Septicemiae) is caused  by Aeromonas hydrophila. The aims of this study was to determine the pathogenicity of bacteria A. hydrophila and effect on total leukocytes from catfish. This research was carried at the Integrated Laboratory and Aquaculture Laboratory, Marine And Fisheries Faculty, Diponegoro University of in May-September 2013. The fish sample was 180 catfish with sizes 7-9 cm. experimental method was applicated. The concentration of  A. hydrophila in treatment A, B, C, D, E, and F were 100 CFU/ml, 105 CFU/ml, 106 CFU/ml, 107 CFU/ml, 108 CFU/ml, and 109 CFU/ml rescpectively. Clinical sign pathogenicity tests were observed done every 6 hours for 96 hours whereas total leukocyte was observed every 24 hours for 6 days. The results showed that clinical sign of catfish infected by A. hydrophila was a decreased in response to feeding, abnormal swimming, injured parts of the body, skin peeling, damaged on the body and abdominal dropsy. Based A. hydrophila on this results of pathogenicity tests with that 96 hours 1,25x106 CFU/ml. the results also showed that highest average total leukocyte was treatment (F) 3.69 x104 cel/mm3 and the lowest was treatment (A) 1.86 x104 cel/mm3.
Pengaruh Ekstrak Daun Sirsak (Annona muricata L) Terhadap Profil Darah dan Kelulushidupan Ikan Lele Sangkuriang (Clarias gariepinus Var. Sangkuriang) yang Diinfeksi Bakteri Aeromonas Hydrophila Kurniawan, Kurniawan; Prayitno, Slamet Budi; Sarjito, Sarjito; Lusiastuti, Angela Mariana
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.253 KB)

Abstract

The main problem in catfish farms is mass mortality coused by Motile Aeromonas Septicemia (MAS) by Aeromonas hydrophila. Natural ingredients that are currently being developed is soursop leaf. It was because soursop leaf contains acetogenin compounds that wishes compared to other plant parts. An acetogenin compound has the benefit of as an antibacterial. The purpose of this research was to determine the influence and best dose of sourshoup leaf extract in feed to the blood profile and survival rate of catfish infected by A. hydrophila. The treatment in this study were the addition of soursop leaf extract on feed with different dosage and 3 replicates. The treatments were A (0 g/kg diet), B (5 g/kg diet), C (10 g/kg diet), D (15 g/kg diet). The result showed that soursop leaf extract in feed of treatment B on the best dose (5 g/kg diet) was total erythrocytes(1,51 x 106 sel/mm3), total leukocytes (8,00 x 103 sel/mm3), limphocytes (92%), monocyte (8%), neutrophils, phagocytic index (75%), and survival rate (93%). It can be concluded that dose of 5 g/kg diet was the best dosage of soursop leaf extract in the feed that was able to stimulate the immune response of catfish, it was characterized by an increased erythrocytes, total leukocytes, differetial leukocyte (limphocytes and monocyte) of catfish infected by A. hydrophila.
Pengaruh Pencelupan Ekstrak Daun Sirih Temurose (Piper betle linn) terhadap Mortalitas dan Histophatologi Ginjal Ikan Mas (Cyprinus carpio) yang Diinfeksi Bakteri Aeromonas hydrophila Istikhanah, -; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.95 KB)

Abstract

Aeromonas hydrophila merupakan salah satu penyebab penyakit bercak merah atau Haemerrhagic  Septicaemia pada ikan mas (Cyprinus carpio) yang saat ini menjadi permasalahan serius dalam budidaya. Salah satu bahan alami yang dapat digunakan  untuk  pengobatan  penyakit  aeromonas adalah  ekstrak daun sirih temurose (Piper betle linn). Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan ekstrak daun sirih temurose terhadap mortalitas dan histopatologi ikan mas yang diinfeksi bakteri A. hydrophila. Penelitian ini dilakukan dalam dua tahap uji, yaitu uji in vitro dan uji in vivo. Pada uji in vivo metode yang digunakan adalah eksperiment laboratoris dengan menggunakan 4 perlakuan yaitu 0 ppm, 800 ppm, 900 ppm, 1000 ppm. Ikan terlebih dahulu diinfeksi dengan cara menyuntikkan 0,1 ml suspense Aeromonas hydrophila sebanyak ±107 cfu/ml secara intramuskular. Setelah menunjukkan gejala klinis, ikan mas direndam dalam ekstrak sirih temurose selama 5 menit yang dilakukan pengulangan 2 kali. Parameter yang diamati adalah gejala klinis, mortalitas dan histologi ginjal dan  ikan mas. Hasil penelitian menunjukkan bahwa gejala klinis ikan mas yang terinfeksi A. hydrophila diantaranya respon pakan menurun, berenang disekitar aerasi, terdapat luka dan daging ikan rusak (nekrosis). Hasil uji in vivo memberikan pengaruh yang berbeda nyata (p<0.05) terhadap mortalitas ikan mas. Konsentrasi yang paling baik adalah 800 ppm (27.29±15.34%), kemudian diikuti perlakuan dengan konsentrasi 900 ppm (37.26±3.41%), konsentrasi 1000 ppm (46.07±22.08%) and 0 ppm (83.86±10.64%). Hasil pengamatan histologi ginjal pada ikan mas menunjukan bahwa pada perlakuan 0 ppm terjadi nekrosis, degenerasi, dan pada perlakuan 800 ppm, 900 ppm dan 1000 ppm terjadi degenerasi. Aeromonas hydrophila is a causative agent of  Haemerrhagic Septicemia on carp which now become a serious problem in fish culture. One of natural materials that can be used to treat disease caused by A. hydrophila is of betle leaf extract (Piper betle linn). The aims of this research were to investigate the effect of betel leaf extract on mortality of carp infected by A. hydrophila and to determine the best dosage to reduce mortality and histopathogy of infected carp. This research was performed by two set of experiments which were in vitro and in vivo study. Dilution  method was used in vitro study by 4 different concentration of betel leav extract which were 0 ppm, 800 ppm, 900 ppm and 1000 ppm. Firstly fish was injected through intramuscularly with 0,1 ml A. hydrophila bacterial suspentoin of 10 7cfu/ml. When the clinical signs were appeared, fish was immersed in various betel leaf extract concentration for 5 minutes and replicated twice. The variables observed were clinical sign, mortalitas, histology of study liver and kidney carp. The results showed Clinical sign of infected carps were swimming abnormally close to aeration,wounds, and haemerrhagi in skin and ulcer make necrosis. In vivo study, betel leaf extract with different concentrations and immersed 5 menitus that given was showed significantly different for the mortality of the carp. The best concentration was 800 ppm (27.29±15.34%), followed by 900 ppm (37.26±3.41%), 1000 ppm (46.07±22.08%) and 0 ppm (83.86±10.64%) respectively. The results of kidney histology observation showed 0 ppm degeneration and necrosis, 800 ppm, 900 ppm and 1000 ppm also showed degeneration.
AGENSIA PENYEBAB PENYAKIT BAKTERI PADA KEPITING BAKAU (Scylla serrata) DARI KENDAL Muna, Nailil; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Vol 3. No 2 (2014): Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.332 KB)

Abstract

Permintaan pasar yang meningkat membuat produksi kepiting bakau (Scylla serrata)  tinggi. Tetapi pada tahun 2011 produksi kepiting bakau di Jawa tengah mengalami penurunan. Salah satu faktor yang mempengaruhinya adalah penyakit. Penyakit bakterial yang menyerang kepiting bakau dapat menyebabkan mortalitas 90%. Penelitian ini bertujuan untuk mengetahui agensia penyebab penyakit bakteri pada kepiting bakau. Materi yang digunakan yaitu 6 ekor kepiting bakau sakit (17,53±0,82 cm) yang berasal dari Kendal. Isolasi bakteri menggunakan media NA, GSP, dan TCBS dengan metode spread plate yang diambil dari karapas (luka), insang, hepatopankreas dan hemolimph sebanyak 1 ml dari 10-1, 10-3 dan 10-5. Isolat murni didapatkan dengan ±3kali ulangan, kemudian disimpan pada NA miring. Hasil isolasi diperoleh 24 isolat bakteri yang kemudian dipilih 7 isolat bakteri secara morfologi untuk postulat koch (SJK1, SJK5, SJK6, SJK11, SJK21, SJK22 dan SJK23). Uji postulat koch menghasilkan tingkatan mortalitas yang beragam yaitu 100%, 66,67-100% dan 33,33-66,67%. Berdasarkan hasil karakterisasi secara morfologi dan biokimia ketujuh isolat agensia penyebab penyakit pada kepiting bakau dari Kendal adalah Vibrio harveyi (SJK1), Moraxella sp. (SJK5), V. ordalii (SJK6 dan SJK11), Staphylococcus delphini (SJK21), Micrococcus luteus (SJK22) dan Pseudomonas putida (SJK23). Increasing market demand of mud crabs (Scylla serrata) makes its production high. But, in 2011, mud crab production in Central Java was declined. One of the inflluence factor was disease outbreak. Bacterial disease that infected mud crab can cause mortality of 90%. This study aims to determine the bacterial agents that cause disease in mud crab. Samples of 6 infected mud crabs with 17.53±0.82 cm length  from Kendal were used in this study. Bacteria was isolated on media NA, GSP and TCBS, under spread plate method taken from the carapace (the wound), gills, hepatopancreas and hemolymph as much as 1 ml of dilution. Pure isolates obtained with 3 replications, then stored in the NA slant. The isolation produced 24 isolates of bacteria. Seven isolates were selected morphologically for postulate koch (SJK1, SJK5, SJK6, SJK11, SJK21, SJK22 and SJK23). Koch postulate resulted in vary mortality levels i.e. 100%, 66.67-100% and 33.33-66.67%. Based on the results of morphological and biochemical characterization, 7 isolated causative agents bacterial disease of mud crabs from Kendal were Vibrio harveyi (SJK1), Moraxella sp. (SJK5), V. ordalii (SJK6 dan SJK11), Staphylococcus delphini (SJK21), Micrococcus luteus (SJK22) dan Pseudomonas putida (SJK23).
PENGARUH KONSENTRASI KONSORSIUM BAKTERI K4, K5 DAN K6 TERHADAP TINGKAT KESEHATAN RUMPUT LAUT (Eucheuma cottonii) Situmorang, Anggun Putriani; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 5, Nomor 1, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.918 KB)

Abstract

Eucheuma cottonii adalah jenis rumput laut yang memiliki nilai ekonomis penting karena memiliki kandungan karaginan yang tinggi (Kappa carageenin dan Lota carageenin). Penyakit ice ice merupakan masalah yang sering dihadapi oleh pembudidaya  E. cottonii. Penelitian ini bertujuan untuk mengetahui pengaruh tingkat konsentrasi konsorsium bakteri penyebab timbulnya penyakit ice ice pada rumput laut (E. cottonii) dan mengetahui gejala klinis rumput laut (E. cottonii) yang terinfeksi konsorsium bakteri. Penelitian dilaksanakan pada bulan  2015. Metode penelitian yang digunakan dalam penelitian ini adalah eksperimental. Rancangan penelitian menggunakan rancangan acak lengkap (RAL), dengan 4 perlakuan dan 3 ulangan. Perlakuan yang diuji adalah konsentrasi konsorsium bakteri yang berbeda dengan perlakuan A (106), B (107), C (108) dan D (100). Rata rata panjang E. cottonii pada penelitian 5 cm dengan bobot rata rata awal 1,5 gr. Pengujian dilakukan selama 22 hari yaitu aklimatisasi 10 hari dan uji patogenitas 12 hari. Hasil penelitian menunjukkan bahwa gejala klinis pada E. cottonii pasca inokulasi bakteri adalah terdapat spot putih pada thallus, pemudaran warna thallus dan bagian permukaan thallus mulai mengelupas, munculnya lendir pada thallus, cabang cabang thallus patah dan akhirnya seluruh bagian thallus patah. Pertumbuhan bobot mutlak rumput laut E. cottonii dalam penelitian selama 12 hari, diketahui bahwa E. cottonii mengalami pertumbuhan negatif. Pertumbuhan negatif paling rendah terjadi pada perlakuan C (-0.90±0.02). Kualitas air selama penelitian tergolong layak untuk kehidupan E. cottonii yaitu suhu pada kisaran 27-29 ˚C, pH pada kisaran 7-8, salinitas pada kisaran 28-30 ppt, nitrat pada kisaran 2,06-0,01 mg/l dan fosfat pada kisaran 1,07-0,2 mg/l. Berdasarkan identifikasi secara biokimia adalah isolat K4 dan K5 teridentifikasi bakteri Baccilus spp dan isolat K6 teridentifikasi bakteri Corynebacterium sp. Eucheuma cottonii is a type of seaweed that has an important economic value because it has a high content of carrageenan (carageenin Kappa and Iota carageenin). Ice- ice disease is a common problem faced by farmers E. cottonii . This study aims to determine the effect of the concentration levels of disease -causing bacteria consortium ice ice on seaweed (E. cottonii) and know the clinical symptoms of seaweed (E. cottonii) were infected with bacterial consortium. The method used in this study is the experimental method. The study design used completely randomized design (CRD), with 4 treatments and 3 replications. Treatments were different concentrations of bacterial consortium with treatment A (106), B (107), C (108) and D (100). Average length of E. cottonii on research 5 cm with an average initial weight of 1.5 gram . Tests conducted over 26 days, 14 days of acclimatization and pathogenicity test 12 days. The results showed that the clinical symptoms of E. cottonii post- inoculation of bacteria is contained white spots on thallus, color fading and surface  thallus began to peel, mucus in the thallus and the media maintenance, broken branches and eventually the entire  thallus broken parts. The growth of the absolute weight of E. cottoniiseaweed in the study for 12 days, it is known that E. cottoniidecreased body weight. The highest decrease in body weight occurred in treatment C (-0.90 ± 0:02). Water quality for relatively decent maintenance for the life of E. cottonii that the temperature in the range of 27-29 °C, pH in the range 7-8 , in the range of 28-30 ppt salinity, nitrate at the end of the maintenance ranging from 2,06-0,01 mg / l and phosphate of the maintenance ranging from 1,07-0,2 mg/l. Based on the biochemical identification of the isolates K4 and K5 are identified Baccilus spp bacteria and bacterial isolates identified K6 Corynebacterium sp .
Identifikasi Bakteri Agensia Penyebab Motile Aeromonas Pada Ikan Lele Dumbo (Clarias gariepinus) yang Berasal dari Kecamatan Rowosari, Kabupaten Kendal Apriliyanti, Pramudita; -, Sarjito; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Vol 2, No 2 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.936 KB)

Abstract

Bacterial disease that often affects catfish was Aeromoniasis caused by Aeromonas sp. This disease was considered as an exotic disease since it reslted in mas mortality over 60% within 7 days. The purpose of this study was to determine the clinical signs of fish sample that affected by bacteria. To identifiy the causative agent of bacterial disesase morphological and biochemically, and then to observe the pathogenicity of the selected motile Aeromonas by postulat koch test. The research method used was a combination between exploration and case study. Purposive random sampling was used to collect moribund catfish. Observation of bacterial clinical signs was carried out to 10 moribund catfish that purposively rondomized. Isolation of bacteria from infected skin and kidney were conducted on aeromonas special medium GSP. Purification was done by recultured the selected colony 3 - 5 times on GSP medium. Three (3) selected isolate for Postulate Koch was cultured in Zobelt medium, and then intramusculary injected to 10 tested catfish of 10 - 15 cm, with concentration 108 clinical response and mortality rates were observed every 6 hours for 96 hours. Finally three tested bacteria were identified morphological and biochemically. The results showed clinical signs that clinical signs of moribund catfish related to Aeromonas infection. This could be seen from the presence of haemorrhagic and pethicia of the body skin such as at abdomen, dorsal and tail fins, exopthlamic and gill necrosis. From the 10 catfishes it could be isolates 16 bacterial colonies. Three selceted isolates namely LPK.GSP3.pk, LPK.GSP4.p and LPK.GSP6.k showed their pathogenicity on the postulat koch test. LPK.GSP6.k demonstrated their pathogen caused 35% mortality and 39% moribund, whilst LPK.GSP3.pk and LPK.GSP4.p equally caused 20% mortality and 47% moribund. Morphoogical and biochemical karacterization of the three isolates above indicated that three isolates closely related to Aeromonas caviae, Aeromonas hydrophila and Aeromonas salmonicida. This was might be the reason why these three bacterial isolates quite virulence.
Co-Authors - Aminah - Desrina, - - Istikhanah - Susanti, - - Triyaningsih Adhi Kurniawan Agatya Sara Ardiantami, Agatya Sara Agil Setya Utomo, Agil Setya Agung Sudaryono Agus Sabdono Agustina Agustina Agustina Alfabetian Condro Haditomo Alfabetian Harjuna Condro Haditomo Alfabetian Harjuno Condro Haditomo Amanda Mega Putri, Amanda Mega Ambaryanto Ambaryanto Amelia Rimadhani ANGELA MARIANA LUSIASTUTI Anggun Putriani Situmorang, Anggun Putriani Aninditia Sabdaningsih Anisa Dwiaryani Latifah Annisa Amalia Awanis Aprilia Dwi Indriani Ardulanisa, Ridha Arif, Gunarso Arifianto Heraedi Asep Akmal Aonullah Asriyana, . Aulia Ayu Ermantianingrum Aulia Resty Wijayanti Ayi Santika Ayu Wulandari Azis Nur Bambang Bagus Oktori Sutrisno cahyadi, jimmy Chyntia Arindita Dani Indrarini Desrina Desrina Desrina, - - Devitha Tri Utami Dewi Nurhayati Dian Ratna Sari Didik Wahju Hendro Tjahjo Edward Raharja, Edward Endah Setyowati Eni Ashfa Ashofa Fajar Purnama, Muhammad Ferdian Bagus Feriandika Fifiana Zulaekah fikih rosalina pratiwi Frida Purwanti Gina Saptiani Gina Saptiani Haditomo, A.H. Condro Hardyta Noviar Rahma Hariyadi, Putut Hasna, Salma Khoironnida Hendrawan, Andika Luky Setiyo Ida Ayu Putu Sri Widnyani Ikhsan Pratama Indah Febry Hastari Intan Eska Amalia Syahida Johannes Hutabarat Kamil, Mia Rahmah Khaerani, Laela Rizki Kurniawan Kurniawan Kusuma, Winanda Adi Laksono Trisnantoro Lestari Lakhsmi Widowati Linuwih Aluh Prastiti Lukman Anugrah Agung Lusi Dianti Marwenni Siregar, Marwenni Max Rudolf Muskananfola Mohammad Saleh Lubis Monica Nanda Muhammad Fajar Purnama Nailil Muna Nida Qolbi Salma Rochani Nita Amelia Nur Annisa Nuri Nia Yanti, Nuri Nia Nurmanita Rismaningsih Nurul Hidayati Ocky Karna Radjasa Peppy Dewi Fitriyanti Pinandoyo Pinandoyo Pramudita Apriliyanti Pricilia, Shella Pujiono Wahyu Purnomo Pungki Nanda Pratama Purnama, Muhammad Putut Har Riyadi R. Dewi Dharina Nurjannah Restiana Wisnu Ariyati Rismaningsih, Nurmanita Ristiawan Agung Nugroho Rohita Sari Rosa Amalia Rusda, Yusrotul Rusydina Qamarul Salikin Sahala Hutabarat Samsul Rizal Sapto P Putro Sarah, Humidah Sarjito - Sekar Ayu Chairunnisa Seto Windarto Shiddieqi, Hasbi Ash Siti Nurjanah Siti Ziyadaturrohmah Solly Aryza Sri Nuryati Sri Rejeki Subandiyono Subandiyono Subhan Subhan Sulisyaningrum Sulisyaningrum Sumbung, Sepri Suryanti Suryanto, Damang Sutrisno Anggoro Syakuri, Hamdan Teguh Eko Suryo Agil Hermawan Tristiana Yuniarti Triwulandari, Atita Utari, Viola Indah Vivi Endar Herawati Widiana, Giacinta Risti Windarto, Seto Yelliana Fatmawati Suwarno Yohanes Kristiawan Artanto YS Darmanto Yunia Dharmastuti Widyaningsih Yusuf Kamlasi Zairin Zain