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All Journal HAYATI Journal of Biosciences SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Althea Medical Journal Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Pharmaceutical Science and Technology MEDISAINS ODONTO Dental Journal Universa Medicina Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Narra J The Avicenna Medical Journal Indonesian Journal of Cancer Journal of Health and Nutrition Research Makara Journal of Health Research Contemporary Journal on Business and Accounting Odonto dental journal Narra X International Journal of Cell and Biomedical Science Indonesian Journal of Biomedicine and Clinical Sciences Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Medical and Pharmaceutical Science
Agung Putra
Faculty of Medicine Universitas Islam Sultan Agung, Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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The Effect of secretome-hypoxia mesenchymal-stem-cells and vitamin-d3 in type-2 diabetes-mellitus induced periodontitis rats Muchaeroni, Isa Anshori; Rochmah, Yayun Siti; Putra, Agung
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.125-131

Abstract

Background: A systemic metabolic disorder by insulin resistance, type-2 diabetes mellitus (T2DM), is characterised by an increased level of reactive oxygen species (ROS) and decreased superoxide dismutase (SOD), which is associated with the activation of proinflammatory cytokine pathways such as tumor necrosis (TNF)-α. This condition usually stimulates inflammation of the periodontal tissue (periodontitis). Vitamin-D3 can inhibit the release of inflammatory mediator and reduce the risk of chronic periodontitis. Purpose this study to determine the effect of the secretome-hypoxia mesenchymal-stem-cells (SH-MSCs)-Vitamin-D3 combination on the expression of SOD, IL-10, and TNF-α genes in T2DM periodontitis-induced rats. Method: 30 rats were randomly divided into five groups; normal sham condition, positive control, SH-MSCs group, vitamin-D3 group, and combination of SH-MSCs and vitamin D3 group. SH-MSCs were injected at doses of 150 uL in the gingival. In addition, vitamin D3 5000 IU 2.25 mcg were administrated orally. After 35 days, all rats were sacrificed, and qRT-PCR from gingival tissue was performed to identify the expression of SOD, IL-10 and TNF-α genes Results: The results a significant increase in SOD and IL-10 gene expression (p
Non-contact electro capacitive cancer therapy (ECCT) modulate the mRNA expression of p53, Apaf-1, survivin, NF-κB, TSP-1 and bFGF in DMBA-induced breast cancer rat Hidayah, Nurul; Putra, Agung; Alamsyah, Firman; Pratiwi, Rarastoeti
Indonesian Journal of Biomedicine and Clinical Sciences Vol 58 No 1 (2026)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v58i1.24954

Abstract

Breast cancer is the most common cancer that causes death in women in the world. Cancer development is facilitated by the inhibition of apoptosis and induction of angiogenesis. Current cancer therapy still encounters problems in the form of recurrence, resistance, and side effects of drugs. Non-contact static electric field therapy, electro capacity cancer therapy (ECCT) with medium frequency, is a therapy developed to inhibit the proliferation of tumor cells. This study aimed to determine the mRNA expression of p53, Apaf-1, survivin related to apoptosis and NF-κB, bFGF and TSP-1 related to angiogenesis in rat breast tumor tissue after ECCT frequency of 150 kHz. Breast tissue samples and rat breast tumor nodules stored in RNA later at -20°C were used. The tissue was obtained from the non-induction non-therapy (NINT) group, induction non-therapy (INT), non-induction therapy (NIT), and induction therapy (IT). mRNA expression of p53, Apaf-1, NF-κB, bFGF and TSP-1 were analyzed using qRT-PCR and calculated with the Livak formula. Data were analyzed using one-way Anova and post-hoc LSD. The results showed that, mRNA expression of p53, Apaf-1 and TSP-1 in the IT group increased significantly, and mRNA expression of survivin and bFGF decreased significantly compared to the INT group. However, the expression of NF-κB mRNA in the IT group remained the same as in the INT group. In conclusion, ECCT with a frequency of 150 kHz upregulates p53, Apaf-1 and TSP-1 mRNA expression and downregulates survivin and bFGF mRNA expression but have no effect on NF-κB mRNA expression in rat breast tumor tissue.
Non-contact electro capacitive cancer therapy (ECCT) modulate the mRNA expression of p53, Apaf-1, survivin, NF-κB, TSP-1 and bFGF in DMBA-induced breast cancer rat Hidayah, Nurul; Putra, Agung; Alamsyah, Firman; Pratiwi, Rarastoeti
Indonesian Journal of Biomedicine and Clinical Sciences Vol 58 No 1 (2026)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v58i1.24954

Abstract

Breast cancer is the most common cancer that causes death in women in the world. Cancer development is facilitated by the inhibition of apoptosis and induction of angiogenesis. Current cancer therapy still encounters problems in the form of recurrence, resistance, and side effects of drugs. Non-contact static electric field therapy, electro capacity cancer therapy (ECCT) with medium frequency, is a therapy developed to inhibit the proliferation of tumor cells. This study aimed to determine the mRNA expression of p53, Apaf-1, survivin related to apoptosis and NF-κB, bFGF and TSP-1 related to angiogenesis in rat breast tumor tissue after ECCT frequency of 150 kHz. Breast tissue samples and rat breast tumor nodules stored in RNA later at -20°C were used. The tissue was obtained from the non-induction non-therapy (NINT) group, induction non-therapy (INT), non-induction therapy (NIT), and induction therapy (IT). mRNA expression of p53, Apaf-1, NF-κB, bFGF and TSP-1 were analyzed using qRT-PCR and calculated with the Livak formula. Data were analyzed using one-way Anova and post-hoc LSD. The results showed that, mRNA expression of p53, Apaf-1 and TSP-1 in the IT group increased significantly, and mRNA expression of survivin and bFGF decreased significantly compared to the INT group. However, the expression of NF-κB mRNA in the IT group remained the same as in the INT group. In conclusion, ECCT with a frequency of 150 kHz upregulates p53, Apaf-1 and TSP-1 mRNA expression and downregulates survivin and bFGF mRNA expression but have no effect on NF-κB mRNA expression in rat breast tumor tissue.
In vivo study on the effect of noni leaf extract cream (Morinda Citrifolia L) on PDGF and TNF-α levels in cut wounds Sari, Shintia Devi Arum; Mulyani, Sri Priyantini; Putra, Agung; Setiawan , Eko
MEDISAINS: Jurnal Ilmiah Ilmu-Ilmu Kesehatan Vol. 23 No. 3 (2025)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v23i3.25252

Abstract

ABSTRACT Background: Skin wounds damage the structure and epithelial tissue, resulting in scar tissue formation. Prolonged wound healing or excessive body response will inhibit the normal process and produce unaesthetic scar tissue. Many plant extracts and their active components can accelerate the wound healing process, Noni leaf extract has antioxidant activity and accelerates wound healing. Objective: This study aims to determine the effect of noni leaf extract cream (Morinda Citrifolia L) on TNF-α and PDGF levels in Wistar rats with incised wounds. Method: A laboratory experimental study with a post-test only control group design. Consisting of five treatment groups, namely the healthy rat group (KS), Negative group (KN) rats with incised wounds without treatment, positive group (KP) rats with incised wounds that were smeared with povidone-iodine cream, treatment group 1 (P1) rats that were smeared with 20% noni leaf extract cream, and treatment group 2 (P2) rats that were smeared with 40% noni leaf extract cream, treatment for 3 days then examined the levels of TNF-α and PDGF skin tissue using the ELISA method. Results: The average TNF-α levels showed a significant difference between groups p of 0.000 (p <0.05) with the One way anova test, the highest average in group (KN) 220.68 pg / mL, there was a decrease in all treatment groups with the lowest TNF-α levels in group (KP) 76.59 pg / mL. The average results of TNF-α levels showed a significant difference of p 0.000 (<0.05) with the One-way ANOVA test, the group without intervention (KP) had the lowest PDGF levels while the group (P2) experienced the highest increase in PDGF levels. Conclusion: Administration of noni leaf extract cream with doses of 20% and 40% had an effect on reducing TNF-α levels and increasing PDGF levels in Wistar rats with incised wounds.
Hypoxia-Conditioned MSC Exosomes Upregulate mTORC1 and Suppress MMP-2 in a UV-B–Induced Collagen Loss Rat Model Fadhillah, Anggrila Sekar; Putra, Agung; Sumarawati, Titiek; Setiawan, Eko
Molecular and Cellular Biomedical Sciences Vol 10, No 1 (2026)
Publisher : Molecular and Cellular Biomedical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v10i1.749

Abstract

Background: Ultraviolet-B (UV-B) radiation accelerates photoaging by disrupting extracellular matrix (ECM) homeostasis through dysregulation of mechanistic target of rapamycin complex 1 (mTORC1) and upregulation of matrix metalloproteinase-2 (MMP-2). This study evaluated the effects of exosomes of hypoxia-conditioned mesenchymal stem cells (EH-MSCs) on mTORC1 and MMP-2 expression in a UV-B–induced collagen loss rat model.Materials and Methods: Thirty male Wistar rats were randomized into five groups: healthy control, UV-B + saline, UV-B + hyaluronic acid, UV-B + 200 µL EH-MSCs, and UV-B + 300 µL EH-MSCs. Collagen loss was induced by UV-B irradiation for two weeks (10 sessions, 8 min/session). A single treatment was administered on day 22, and tissue was collected on day 29. Exosomes were isolated from hypoxia-conditioned MSCs and characterized by morphology and surface markers. Gene expression of mTORC1 and MMP-2 was assessed by qRT-PCR and analyzed using one-way ANOVA.Results: UV-B exposure induced collagen loss histologically. EH-MSCs significantly increased mTORC1 expression, highest in the 300 µL group (p < 0.001), and reduced MMP-2 expression, lowest in the 300 µL group.Conclusion: EH-MSCs exert dual regulatory effects by upregulating mTORC1 and suppressing MMP-2 in UV-B–induced collagen loss, suggesting therapeutic potential to mitigate photoaging via anabolic signaling (via mTORC1) and reduced ECM degradation (via MMP-2).Keywords: collagen loss, exosomes, MMP-2, mTORC1, UV-B
Hypoxic MSCs Reduce UV-B Collagen Loss by Modulating CD68 and TNF-α Expression Ati, Sri Umi; Putra, Agung; Sumarawati, Titiek; Setiawan, Eko; Ibrahim, Sugeng; Taskworo, Dodik
Indonesian Journal of Pharmaceutical Science and Technology Vol 13, No 1 (2026)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v13i1.63650

Abstract

Ultraviolet-B (UV-B) radiation-induced skin photoaging is marked by collagen degradation and chronic inflammation, with limited effective treatments currently available. Hypoxic-preconditioned mesenchymal stem cells (H-MSCs) offer a novel therapeutic approach due to their immunomodulatory capabilities. This study evaluated the effects of H-MSCs at two doses (2.5×105 and 5×105 cells) on UVB-induced collagen loss, focusing on CD68 expression and TNF-α levels in a rat model. Male Wistar rats were divided into five groups: healthy controls, UV-B-exposed negative controls (saline), positive controls (hyaluronic acid), and two H-MSC-treated groups. After UV-B exposure (160 mJ/cm², five times per week for two weeks), validated H-MSCs were administered subcutaneously. Collagen content was assessed histologically, while CD68 gene expression and TNF-α levels were measured by qRT-PCR and ELISA, respectively. UVB exposure led to significant reductions in collagen and increased levels of inflammatory markers. H-MSC treatment showed dose-dependent anti-inflammatory effects, with the higher dose (5×105 cells) optimally reducing CD68 expression and TNF-α levels, nearly matching healthy controls. These results suggest that H-MSCs, particularly at higher doses, may be a promising therapy for UVB-induced skin damage and collagen loss.
Co-Authors Agus Widyatmoko, Agus Alif, Iffan Amalina, Nur D. Amalina, Nur Dina Amin, Mustafa M. Andavania, Sheila Jessica Andreas, Yana Antari, Arini Dewi Ati, Sri Umi Azizah Retno Kustiyah Cahyani, Dini Cahyani, Elvana Cahyono, Erwin Budi Candra Satria Irawan, Risky Catharina Suharti Chodidjah Chodidjah Chodijah , Chodijah Darlan, Dewi M. Daulay, Rini S. Delfitri Munir Dewi, Alisia Martha Dirja, Bayu T. Dirja, Bayu Tirta Djannah, Durrotul Edi Dharmana Eko Setiawan Eko Setiawan Erna Mutiara Evita Mayasari Fadhillah, Anggrila Sekar Fahreza, Rakha Fatmawati, Dian Fikriya Novita Sari Firman Alamsyah Fristiani, Yeni Ghaisani, Shabrina Syifa Ginting, Andi R. Hadi Sarosa Haitamy, Mohammad Nurrizki Handoyo, Frigi Eko Hariani, Nova Putri Hasanal, Ihdina Hanifa Hasannuri, Tarrayuana Rhamadia Heri Nugroho HS, Zakariya Husain, Sofian A. Hutabarat, Nenny Lynda Caroline Hutagalung, Ananta Ibrahim, Sugeng Ignatius Riwanto, Ignatius Intan, Yulice Soraya Nur Irawan Mangunatmadja Irawan, Risky Chandra Irawan, Risky Chandra Satria Isfandiari, Adelia Bayu Iskandar Japardi Jessika, Cleveria Khan, Ahmed Faheem Kuntardjo, Novalia Lusiana Lusiana Mardiana, Ana Maryanti Maryanti Mawarini, Melisa Septi Minidian Fasitasari Muchaeroni, Isa Anshori Muhar, Adi Muradi Noka, Isara Abda Nugraha, Dendi Krisna Nur Anna Chalimah Sadyah NURUL HIDAYAH Nurul Hidayah Pasongka, Zenitalia Pramukarso, Dodik Tugasworo Pramukarso Prasetio, Ardi Prasetyowati Subchan, Prasetyowati Prawitasari, Salindri Purwaningsih, Hesti Rahardja, Carolina Kiwik Rarastoeti Pratiwi Rozi, Muhammad F. Rusda, Muhammad Sa’dyah, Nur Anna C Sari, Shintia Devi Arum Selamat Budijitno Setiawan , Eko Setyo Trisnadi Setyo, Trisnadi Shafia, Arina Sisca Silvana, Sisca Sitompul, Faya Nuralda Sri Priyantini Sri Priyantini Mulyani Sri Sofyani, Sri Subchan, Prastyowati Sulistami, Siska Marlina Sulistyo, Sona Sunarto, Hadi Sutrisman, Intan Permatasari Suwandi Ng Syamsunarno, Mas Rizky Syamsunarno, Mas Rizky A.A Taskworo, Dodik Taufiq R Nasihun, Taufiq R Titiek Sumarawati Tjipta, Arya Trisnasi, Setyo Utami, Wulan Dyah Yasmine Azzahara, Salma Yayun Siti Rochmah Yustianingsih, Vivi