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The Potency of Trigona’s Propolis Extract as Reactive Oxygen Species Inhibitor in Diabetic Mice Ahmad Ridwan; Ayu Nirmala Sari; Ramadhani Eka Putra
Journal of Mathematical and Fundamental Sciences Vol. 47 No. 3 (2015)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2015.47.3.4

Abstract

Hyperglycemia has been proven to increase oxidative stress due to the production of reactive oxygen species (ROS) that exceed the capabilities of the natural antioxidant defenses, causing a deficiency in insulin receptors and insulin resistance. In this study, the effect of propolis on ROS was observed. Fifty five (55) male mice (Mus musculus SW.) were divided into 5 groups, i.e. KN (normal control), KDM (diabetes control), and P1, P2, P3. Propolis solution 50, 100 and 175 mg/kg bw was given to groups P1, P2 and P3 respectively, while distilled water was given to groups KN and KDM by oral gavage for 21 days. Density of ROS was measured every 7 days, while measurement of plasma insulin was carried out every 3 days. The results show that the density of ROS in the groups treated with propolis was lower than in the KDM group. However, the plasma insulin levels in the propolis groups were higher than in the KDM group. It was concluded that propolis can decrease ROS density and causes an increase in plasma insulin levels.
Pertumbuhan dan perkembangan larva Musca domestica Linnaeus (Diptera: Muscidae) dalam beberapa jenis kotoran ternak Ramadhani Eka Putra; Abdul Rosyad; Ida Kinasih
Jurnal Entomologi Indonesia Vol 10 No 1 (2013): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.592 KB) | DOI: 10.5994/jei.10.1.31

Abstract

House fly (Musca domestica) is an important urban insect that can transmit various infectious diseases. This insect usually utilized organic wastes as nutrition source for their larvae. One of the main sources of organic wastes is livestock manure produced by animal farming located near human dwelling area. Thus, appropriate house fly population control program at animal farm is needed,based on information on the house fly’s life history. The research is focused on the development of house fly larvae reared with different livestock manures, such as cow, chicken, and horse. As comparison, rice bran were used as control. Results showed that larvae reared with horse manure has the shortest development time (5 days), with lowest larval survival rate (30%), pupal weight (6.8 ± 0.141 g), and weight of female imago (4.9 ± 0.14 g). This finding indicates the lowest nutrition value of horse manure for house flies larvae. Further research is needed to find the effect of manure to variables that directly influence population growth, such as fecundity of female flies and egg survivorship. These additional information on life history will help the design of appropriate house fly population management program for animal farm.
Aplikasi campuran serbuk kayu pinus dan fipronil sebagai umpan rayap tanah Macrotermes gilvus (Hagen) (Isoptera: Termitidae) di Bandung Amran Amran; Intan Ahmad; Ramadhani Eka Putra; Eko Kuswanto
Jurnal Entomologi Indonesia Vol 12 No 2 (2015): Juli
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.777 KB) | DOI: 10.5994/jei.12.2.73

Abstract

In this study, termite bait comprising pinewood of sawdust and a slow action insecticide, fipronil, was made and applied to control subterranean termite Macrotermes gilvus (Hagen) (Isoptera: Termitidae) in Bandung. Colony size was measured using capture-mark-release-recapture prior to the bait application, along with the measurement of wood consumption at each station.  Colony foraging populations at all stations were estimated to be 77,951 termites, with mean wood consumption rates ranging from 0.02 to 6.16 g/station/day. Observation at 18 stations installed with bait consisted of 40 ppm fipronil mixed with pinewood sawdust showed that number of foraging activity termites was effectively reduced in 40 days. It is concluded that fipronil-treated pinewood sawdust bait is effective in controlling the population of foraging M. gilvus workers in 40 days.
Toksisitas beberapa jenis fungisida komersial pada serangga penyerbuk, Trigona (Tetragonula) laeviceps Smith Ida Kinasih; Rusdy Syachrul Nugraha; Ramadhani Eka Putra; Agus Dana Permana; Mia Rosmiati
Jurnal Entomologi Indonesia Vol 14 No 1 (2017): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.26 KB) | DOI: 10.5994/jei.14.1.29

Abstract

Fungicide is one of the common pesticide applies in agriculture. Even though fungicide is specifically designed to prevent growth of fungi, some studies reported neurotoxic effects of fungicide to some tested animals. Therefore there are concerns about the possible determintal effects of fungicide to beneficial non target organisms, like pollinating insects. This study was conducted to determine the toxicity of commercial fungicide to pollinator, Trigona (Tetrgonula) laeviceps Smith, by determining the LD50 and hazard quotient (HQ). The fungicides tested were triazole (tridimefon and difenoconazole), propamorcab (propamocarb HCl), and dithiocarbamate (mancozeb and propineb). Forager bees were collected and anasthesized by exposing the bees to low temperature (-10 oC) for 1 min. About 1 µl of tiadimefon (6,25; 12,5; 25; 37,25; 50 µg/bee), difenoconazole (1,25; 2,5; 5; 6,25; 12,5; 25 µg/bee), propamocarb HCl (72.,25; 144,5; 216,6; 361,25; 433,5 µg/bee), mancozeb (1,6; 3,2; 4,8; 6,4; 8 µg/bee), and propineb (0.9; 1.8; 2.7; 6; 8.4; 16.8; 25.2 µg/bee) were applied using topical application on dorsal area of bees. Ten bees were used for each concentration and replicated 3 times. Mortality of bees was recorded for 48 hours. Results showed that LD50 and HQ for each tested fungicide were as follow: triadimefon LD50 13.43 ±1.78 µg/bee, HQ = 18.62; difenoconazole LD50 9.25 ± 1.21 µg/bee, HQ = 27.03; propamocarb HCl LD50 270.45 ± 19.48 µg/bee, HQ = 14.34; mancozeb LD50 3.75±0.47 µg/bee, HQ = 512; and propineb LD50 9.71 ±2.20 µg/bee, HQ = 144.18. Among all 5 tested fungicide, two fungicide, mancozeb and propineb, has intermediate level of toxicity to foragers of T. laeviceps and could produce determintal effect to colony.
Pola kunjungan serangga liar dan efek penambahan koloni Trigona (Tetragonula) laeviceps Smith pada penyerbukan kabocha (Cucurbita maxima) Ramadhani Eka Putra; Julio Subagio; Ida Kinasih; Agus Dana Permana; Mia Rosmiati
Jurnal Entomologi Indonesia Vol 14 No 2 (2017): Juli
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.983 KB) | DOI: 10.5994/jei.14.2.69

Abstract

Insects hold important role in pollination of many crops. However, studies on pollination in Indonesia could be considered rare. This study focussed on obtaining the basic information of wild pollinators and the effect Trigona (Tetragonula) laeviceps Smith innundation release to the production and quality of pumpkin (C. maxima) in traditional farming system. Observations were carried out from 07:00 to 15:00 during flowering period for 3 weeks. Sampling area was divided into three plots : open pollination + T. laeviceps (OPT), open pollination (OP), and hand pollination (HP). Pollination was assessed by three main parameters, which were visitation pattern, plant reproductive success, and fruit quality. Visitation pattern was assessed by measuring frequency and duration of pollinator visits, the plant reproductive success by fruit set and number of seed produced; while fruit quality by diameter, weight, and number of fruit produced/plant. We recorded different visitation pattern in which wild pollinator preferred to visit flowers in the morning while T. laeviceps preferred to visit from noon to afternoon. This pattern could be relate to difference in thermal tolerance among pollinators. There were significant differences among fruit set produced in OPT (80%), OP (65%), and HP (38.3%) (F2,35 = 19,907, P = 0,000). Number of seeds and fruit quality produced in OPT and OP much higher than HP while number of fruits produced in OPT significantly higher that OP and HP. Low pollination success in HP showed pollination process in pumpkin highly depend on pollination time. Thus, pollination by bees in pumpkin hard to be replace by human and variation of visit provided by T. laeviceps produce positive effect to pumpkin.
Efek pemberian pakan berlebih berupa limbah sayuran pakcoy terhadap daya cerna, tingkat penurunan limbah, dan kandungan protein pada lalat tentara hitam (Hermetia illucens (Linnaeus)) Agus Dana Permana; Ramadhani Eka Putra; Raeka Okata Soebakti; Ida Kinasih
Jurnal Entomologi Indonesia Vol 18 No 3 (2021): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.18.3.170

Abstract

One of the recent organic waste management programs actively developed is organic waste bioconversion by using larvae of black soldier fly (Hermetia illucens (Linnaeus)). However, one major challenge in organic waste management in Indonesia is the limited space to manage the wastes at waste collection centers. This condition increases the number of wastes to be converted (consumed) by larvae of soldier fly. In this study, the condition was imitated in the laboratory where larvae were individually fed with a large number of wastes. Larvae were divided into 4 groups and were fed with 200, 300, 400, and 500 mg/larva/day of rotten vegetable waste (Pakcoy) and were replicated 3 times. Variables observed during the study were the growth rate, consumption capability, level of waste reduction (waste reduction index, WRI), consumption efficiency (efficiency of conversion of digested-feed, ECD), the weight of harvested biomass, and protein content of larvae, pupae, and adults. The results showed that there was no significant correlation between the weight of harvested biomass and the amount of substrate provided to larvae (one-way ANOVA, P > 0.05). The increasing amount of substrate reduced the efficiency of waste reduction and consumption (one-way ANOVA, P < 0.05). This study also showed that the possible upper limit of consumption capability of larvae was 400 mg/larva/day. On the other hand, the increasing amount of substrate increased the protein content of biomass of each developmental stage. 
The Protective Effect of Propolis Nanoemulsion (NEP) Against UVB Irradiation Induced Photoaging in Human Dermal Fibroblast (HDF) Anggraini Barlian; Rini Agustin; Ramadhani Eka Putra
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 2 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.2.1

Abstract

The skin damage induced by UV irradiation can cause photoaging as a consequence of reactive oxygen species (ROS) generation. Propolis is one of the most abundant natural product sources of polyphenols and derived from various plant resins collected by honeybees. This study aimed to determine the due administration of the propolis nanoemulsion (NEP) as an anti-photoaging by observing the concentration of intracellular ROS and lipid peroxides in human dermal fibroblast (HDF) by exposure to UVB. The cytotoxic of NEP was tested by the MTT assay, intracellular ROS was measured using the DCFDA assay, lipid peroxide products were analyzed by the TBARS assay and cell nuclei was observed by DAPI staining. PSA analysis revealed that the sizes of NEP were about 131.9 to 131 nm. The optimum concentration of NEP was 0,1μg / ml. ROS production in HDF treatment of NEP significantly decreases (p <0.05). Lipid peroxide products showed the same pattern as ROS concentration. The lipid peroxide concentration in the treatment NEP significantly decreases (p<0.05). In conclusion, the propolis in NEP acts as an antioxidant and has the potential to reduce the production of ROS and lipid peroxides caused by exposure to UVB.
Wheatgrass microgreen with high antioxidants content in an urban indoor farming system Myrea Chalil; Karlia Meitha; Ramadhani Eka Putra; Fathia Aulia Rahmah; Ridho R Sinatra; Anindha Ajeng Putri Winanta
3BIO: Journal of Biological Science, Technology and Management Vol. 4 No. 1 (2022)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2022.4.1.4

Abstract

Urban lifestyle is identical to stressful life and sedentary habit, leading to the increase of chronic conditions such as diabetes and cardiovascular-related diseases. Antioxidants are renowned for maintaining cellular function by quenching radicals produced in stressful conditions or infection. Fresh fruits and vegetables are the primary sources of antioxidants, but the long postharvest and transport system may reduce the benefits for the urban population. Hence, we designed a cultivation method to produce wheatgrass microgreens with high antioxidants in an urban indoor farming system. Generally, plants require light at the wavelength of 663 and 642 nm (red) and 430 nm and 453 nm (blue) to allow photosynthesis and production of secondary metabolites, such as antioxidants. We applied the LED lights with an RGB ratio of 91R/9B, 83R/17B, 47R/53B, 35R/65B, and white florescent as the control. Our results showed that 91R/9B reduced fresh mass and chlorophyll content, which might be due to the suppression of photosynthesis capacity. Interestingly, we found a significant (p<0.05) increase in carotenoids and flavonoid contents due to light combinations of 35R/65B and 83R/17B, respectively. However, the total antioxidants capacity was similar among all treatments. Carotenoids and flavonoids are among the antioxidants with a significant role in decreasing the risks of chronic diseases and their potential as antiviral agents. This cultivation system of wheat microgreen could be a promising solution to routinely supply carotenoids and flavonoids to the urban population. Further, it is also considered more environmentally friendly as it could be performed in a limited amount of land (vertically) and potentially use less energy for distribution.
Diversity and Abundance of Insects Pollinator of Chayote (Sechium edule (Jacq.) Swartz Muhammad Dzaky Alfawwaz; Agus Dana Permana; Ramadhani Eka Putra
Jurnal Biodjati Vol 7, No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.13881

Abstract

Chayote plants (Sechium edule) with monoecious characters require a pollination process. The pollination process requires pollinating agents to increase its productivity, one of which is insects. This research aimed to determine the diversity and abundance of insects pollinator on chayote plants. Observation of diversity and abundance used a scan sampling method. Pollinator insects observations were carried out in 3 time periods, morning, afternoon, and evening on male and female flowers. We measured environmental parameters such as temperature, humidity, wind speed, and light intensity. Eight species of wild insects pollinated chayote flowers, Apis cerana, Apis dorsata, Lasioglossum leucozonium, Polistes sagittarius, Phimenes flavopictus, Campsomeriella annulata, Lucilia sericata, and Musca domestica. The insect pollinators community had moderate diversity (1.23), a relatively dynamic community (0.59), and moderate dominance (0.62), with A. cerana, which had been the dominant insect pollinator with a relative abundance of 61.63%. Musca domestica and L. sericata were (0,58%) the least dominant insect pollinator with a relative abundance of 0.58%. This research concludes that the insects pollinator of chayote has a moderate level of diversity, relatively dynamic community, and average dominance.
Dynamics of Competitiveness and Efficiency of Rice Farming in Java Island, Indonesia Amrina Rosyada; Ramadhani Eka Putra; Wawan Gunawan
3BIO: Journal of Biological Science, Technology and Management Vol. 4 No. 2 (2022)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2022.4.2.5

Abstract

Rice is the major crop in Indonesia and the staple food for more than 90% of Indonesians. Given the vital role of rice, efforts to develop rice production are a priority, especially in solving farming efficiency problems. However, inefficiency is one of the major causes of low performance in Indonesian rice production. If farming has high competitiveness and efficiency, Indonesia is encouraged to be able to become an exporter of rice. As a result, national rice farming must continue to improve its competitiveness and efficiency. This study aims to determine the economic efficiency and competitiveness of rice farming in several provinces in Java, i.e., West Java, Central Java, and East Java. The data used in this study is PATANAS survey data obtained from the Center for Socio-Economic Studies and Agricultural Policy, Indonesian Ministry of Agriculture. This study used a quantitative analysis approach and analytical descriptive analysis. The level of competitiveness was analyzed using Policy Analysis Matrix (PAM), while the efficiency level was analyzed using the Stochastic Frontier Method (SFM). The results showed that rice farming in Java Island has a positive profit value on private and social prices. In addition, rice farming in all research locations has competitiveness as measured by indicators of comparative and competitive advantage as characterized by the coefficient values ​​of DRC (Domestic Resource Cost Ratio) and PCR (Private Cost Ratio), which are less than one in the study period 2007-2020. The result of a technical efficiency study shows the average technical efficiency of three provinces in Java is around 0.82, and the factor input that significantly increased the technical efficiency was land and intermediate input.
Co-Authors Abdul Rosyad Acep Purqon Achadiyani Adriyanita Adin Agnita Ratnasari Agus Dana Permana Agus Susanto Ahmad Ridwan Alfariana Margareta Ali Agus Amran Amran Amrina Rosyada Amrulloh, Rosyid Ana Rochana Andi Gita Maulidyah Indraswari Suhri Anggraeni, Raden Dewi Anggraini Barlian Anindha Ajeng Putri Winanta ANJARNISA RUSTAM, FAJRINA Aos Araz Meilin Armowidi, Tri Ateng Supriyatna Auliya Nurulfah Ayu Nirmala Sari Ayuningrum, Sri Utami Christanto, Billy D Beta Ramadan Damanik, Naomi Florenata DAMAYANTI BUCHORI Daniel Happy Putra Denny Rusmana DEWI SARTIAMI Din Dzakamala Fafi Rohmatillah1 Djati, Imam Damar Dwiartama, Angga Dwika Bramasta Eko Kuswanto Fahri Rijal Giffari Fathia Aulia Rahmah Fauziah, Nisa Finsa Firlana Gusmara Fitria Nur Aisyah Gres Maretta, Gres Gunadi Trinuroni Hani Hidayah Hanifah, Dini Siti Hasna Safira Amnevi Heri Rahman Heri Rahman, Heri Hery Purnobasuki Hufaizah, Pajar Husna, Inayati Zakiyatun IBNUL QAYIM Ida Kinasih Ika Fitriya Imam Fathurrahman Intan Ahmad Intan Josefin Purba Jennifer Adelia Latif Julio Subagio Kamilah, Mutiara Mila Karlia Meitha Latama, Zahra Nabila Leksikowati, Sovia Santi Lia Faridah Lutfiah Alfariza Margareta, Alfariana Meliyani, Auliya Tri Muhammad Dzaky Alfawwaz Muhammad Yusuf Nurhadi Myrea Chalil Nadia Damika Putri Nasution, Ahmad Raihan Natalia Eka Prasetia Nina Ratna Djuita Nindia Safa&#039;at Novitasari Novitasari Novitasari Nuradzkia, Nida Nurdiansyah, Muhammad Aldi Octaviani, Nurul Walidah Octy Viali Zahara Oktaviani, Indah Pujo, Pujo Purqon, Acep Putri, Nadia Damika R.C. Hidayat Soesilohadi Rachel Anggia Rachmat Wiradimadja Raeka Okata Soebakti Rahmani, Aena Sania Ramadhani Eka Putra Ramadini Aini Anitasari RC Hidayat Soesilohadi Ricky Selamet Rahayu Ridho R Sinatra Rika Alfianny Rika Alfianny RIKA RAFFIUDIN Rini Agustin Rohmat, Farhan Ilham Wira Rosmiati, Mia Rusdy Syachrul Nugraha Santi Elvira SARI, ADHA Sari, Anggraeni Arum Sari, Dian Anggria Sari, Dian Anggria Satrio Aribowo B. Wicaksono Savira Ekawardhani Setiyarni, Eka SETYARNI, EKA Sih Kahono Sih Kahono Sofiatin Sparisoma Viridi Tien Lastini Tjandra Anggraeni TUTUT INDAH SULISTIYOWATI Ucu Julita, Ucu Untia Kartika Sari Ramadhani Watanabe, Kozo Wawan Gunawan Wawan Gunawan WINDRA PRIAWANDIPUTRA, WINDRA Yenyen Fatmalasari Yeyet Setiawati ZAKIYATUN USNA, INAYATI