Article Per Year (5 Year)
p-Index From 2021 - 2026
3.441
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Majalah Kedokteran Bandung Jurnal Keperawatan Jiwa (JKJ): Persatuan Perawat Nasional Indonesia Majalah Obstetri dan Ginekologi Majalah Kesehatan FKUB Warta Ardhia : Jurnal Perhubungan Udara Medical Laboratory Technology Journal Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Care : Jurnal Ilmiah Ilmu Kesehatan Journal of Issues in Midwifery Heart Science Journal Devotion: Journal of Research and Community Service Narra J Indonesian Journal of Perinatology Asian Journal of Health Research Jurnal Info Kesehatan
Bambang Rahardjo
Faculty Of Medicine Universitas Brawijaya Dr. Saiful Anwar General Hospital Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Transportation Veterinary

Published : 30 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 30 Documents
Search

 1   2   3 
The Effect of Turmeric Decoctum to the Angiogenic Molecules Expression on Chicken Embryo Sultanah Zahariah; Sri Winarsih; Siti Candra Windu Baktiyani; Bambang Rahardjo; Umi Kalsum
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.10

Abstract

Turmeric (Curcuma longa) is widely used as herbal medicine, not an exception by pregnant women. Turmeric consumption by expectant mothers requires standard dose, because of its antiangiogenic effect could be harmful on placentation process and embryonic development. This experiment was undertaken to determine the effect of different concentrations of turmeric decoctum to the expression of Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2) and Angiopoietin 1 (Ang-1) on the 48-hours-old chicken embryo. In this study, turmeric was extracted using decoction method to mimic the common method as adopted by people. The turmeric decoctum were freeze dried into a powder form and was used in preparing the stock solution for 200 ppm (P1), 300 ppm (P2), and 400 ppm (P3) as experimental treatments. The control group (P0) received 2% DMSO without turmeric decoctum. These were administered on the yolk sack of 16 hours incubation of fertile chicken egg by number of 200 µL. After 48 hours incubation, the expression of VEGFR-2 and Ang-1 on the chicken embryo were counted by ImageJ software. The results revealed that there is no significant effect of turmeric decoctum to the expression of VEGFR-2 and Ang-1. This suggested that turmeric decoctum was safe up to 400 ppm on chicken embryo.
Toxicity Test on Wedusan (Ageratum spp.) Extract Against Sitophilus spp. (Coleoptera: Curculionidae) Erinus Mosip; Bambang Rahardjo; Ludji Astuti
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tropical condition in Southeast Asia has contributed around 30% of post harvest damage as it provide optimum condition for the growth of Sitophilus spp. pest. Therefore, this study was aimed to investigate the effect of wedusan extract on controlling Sitophilus spp. Growth. Wedusan extract was given in different level of concentrations. The research used the Randomized Complete Design. Wedusan (Ageratum spp.) extract affected the imago mortality of Sitophilus spp. There were two kinds of Wedusan extract used in this study, dried extract (DE) and fresh extract (FE).  Sitophilus imago were put into bottles filled with organic rice and were treated with Wedusan extract. The mortality level was obsered at 24 Hours After Application (HAA), 48 HAA, and 72 HAA. The highest toxicity was found by the treatment of dried extract of wedusan 5 tablets, by LC50 55% on 24 HAA and 82.25% on 72 HAA. Extract of dry matter has higher toxicity effect than fresh extract. The application of wedusan extract is eviromental friendly and specific-range against pest. It is promising for future development as natural product in agriculture area.
Effect of Phaleria macrocarpa Extract on NF-KB, MMP-2, and MMP-9 Expression in Endometriosis Mice Model Annissa Febriani; Sutrisno Sutrisno; Yahya Irwanto; Irfani Baihaqi; I Wayan Arsana Wiyasa; Bambang Rahardjo
Asian Journal of Health Research Vol. 1 No. 3 (2022): Volume 1 No 3 (December) 2022
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v1i3.35

Abstract

Introduction: Endometriosis is the most common disease that affects the reproductive health and function of women of reproductive age. Treatment for endometriosis includes surgery and medication. Phaleria macrocarpa is a plant native to Indonesia that contains bioactive fractions known to have antiproliferative and apoptotic effects. Therefore, this study aimed to evaluate the effect of Phaleria macrocarpa extract on matrix metalloproteinases (MMP-2 and MMP-9) and NF-KB expression in the endometriosis mice model (Mus musculus). Methods: This study used a Randomized Post-Test Only with Control Group Design. Involves six groups, namely a negative control group (healthy mice without flavonoids from Phaleria macrocarpa fruit extract), a positive control group (an endometriosis model without being given flavonoids from Phaleria macrocarpa fruit extract), and a treatment group, namely a group given four different doses of flavonoids from Phaleria macrocarpa fruit extract: 3.75 milligrams per day, 7.5 milligrams per day, 11.25 milligrams per day and 15 milligrams per day. expressions of NF-KB, MMP-2, and MMP-9 were seen using Immunohistochemistry staining and observed under a microscope with 40x magnification. The data collection used an immunoratio technique with ImageJ analysis software. Furthermore, data analysis using the one-way ANOVA method. Results: In this study, the treatment group with four different doses of Phaleria macrocarpa extract could reduce the expression of MMP-2, MMP-9, and NF-KB. Groups with higher doses of Phaleria macrocarpa extract showed lessen of MMP-2, MMP-9, and NF-KB expression. Conclusion: In the endometriosis mice model, Phaleria macrocarpa fruit can reduce NF-KB, MMP-2, and MMP-9 expression.
Exploring The Relationship Between Lactate Dehydrogenase Levels, Leukocyte Counts, Low Birth Weight, and The Severity of Preeclampsia in Pregnant Women: A Cross-Sectional Study at Saiful Anwar Hospital Malang (July 2021 to June 2022) Bambang Rahardjo; Talita Nandia Primarintan
Asian Journal of Health Research Vol. 2 No. 2 (2023): Volume 2 No 2 (August) 2023
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v2i2.112

Abstract

Introduction: This study investigates the connection between lactate dehydrogenase (LDH) levels, leukocyte counts, low birth weight, and the severity of preeclampsia among pregnant women. Conducted at Saiful Anwar Hospital in Malang from July 2021 to June 2022. Material and Methods: This research utilizes a descriptive and analytical observational approach with a cross-sectional design. Pregnant women meeting specific criteria were evaluated for LDH levels, leukocyte counts, preeclampsia severity, and newborn birth weights. Statistical analyses, including the Kruskal-Wallis test and Spearman correlation, were employed to explore relationships and significance among variables. Results: There is significant differences in LDH levels and low birth weight across patients with mild preeclampsia, severe preeclampsia, and eclampsia. LDH levels were linked to preeclampsia severity, while low birth weight correlated with eclampsia. Leukocyte levels, however, did not exhibit notable differences among these group. Conclusion: This study underscores the necessity of early assessment and monitoring of these factors in pregnant women, aiming to enhance maternal and fetal outcomes. Further research is recommended to validate these findings in diverse populations and explore additional risk factors associated with preeclampsia-related conditions. In conclusion, this research illuminates the interplay between LDH levels, leukocyte counts, low birth weight, and preeclampsia severity. The outcomes emphasize their importance as potential markers for evaluating and managing preeclampsia-related complications.
Correlation between Secreted Aspartyl Protease-5 (SAP5) on Increasing Vaginal Acidity in Vulvovaginal Candidiasis Nur Sophia Matin; Regina Ayu Fristiyanti; Safrina Dewi Ratnaningrum; Nurdiana Nurdiana; Sanarto Santoso; Bambang Rahardjo
Asian Journal of Health Research Vol. 2 No. 2 (2023): Volume 2 No 2 (August) 2023
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v2i2.116

Abstract

Introduction: Vulvovaginal candidiasis (VVC) is an infection of the vaginal mucosa that extends to the vulva due to excessive growth of Candida albicans, causing a progressive decrease of Lactobacillus spp., as the main of normal flora. Decrease in the number of Lactobacillus spp. causes a reduction of lactic acid production that it was causing increasing vaginal pH. The increase in pH is due to the progressive growth of C. albicans colonies in the vaginal mucosa. In addition, the mechanism of candidiasis is also influenced by the secretion of virulence factors by C. albicans. Secreted Aspartyl Protease-5 (SAP5) is the dominant virulence factor that plays a role in changing the morphology of yeast cells into hyphae. This study aims to identify the correlation between SAP5 levels and vaginal pH. Material and Methods: This research was conducted in vivo using a sample of Rattus norvegicus with a posttest randomized experimental group design approach. This study has two groups, i.e., Negative Control (NC) and Positive Control group (PC). SAP5 and pH samples were taken from vaginal fluids and then measured using ELISA and a digital pH meter. The statistical analysis used was Pearson Correlation to analyze the correlation between SAP5 and pH levels. Results: There was a strong correlation (R=0.846) that means the increase of SAP5 is related to the increase of vaginal acidity in the PC group. Conclusion: The increase in SAP5 levels is directly followed by an increase in the pH value of vaginal fluids,
JUMLAH MONOSIT, KADAR INTERLEUKIN-6, DAN KADAR FAKTOR PENGHAMBAT MIGRASI MAKROFAG LEBIH TINGGI PADA KONDISI PREEKLAMPSIA DENGAN SEPSIS Rahardjo, Bambang; Mustofa, Edy; Sanggelorang, Margie Cassie; Wati, Linda Ratna
Majalah Kesehatan Vol. 11 No. 2 (2024): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/majalahkesehatan.2024.011.02.1

Abstract

Preeklampsia merupakan komplikasi kehamilan yang ditandai dengan peningkatan tekanan darah >140/90 mmHg, proteinuria, dan edema, yang terjadi setelah 20 minggu kehamilan. Penyebab terjadinya preeklampsia tidak diketahui secara pasti, tetapi kemungkinan terkait dengan respons inflamasi sistemik. Monosit dan makrofag yang telah distimulasi oleh endotoksin akan menghasilkan interleukin-6 (IL-6) yang dapat  memicu respons inflamasi berlebih, dan mungkin memainkan peran sentral dalam respons inflamasi pada preeklampsia dan sepsis.  Tujuan penelitian ini adalah untuk membandingkan jumlah sel monosit, kadar IL-6, dan kadar macrophage migration inhibitory factors (MIF) pada preeklampsia dan preeklamsia dengan sepsis. Penelitian ini menggunakan desain penelitian cross-sectional. Sampel darah dikumpulkan dari partisipan di RSUD dr. Saiful Anwar Malang dan Bangil yang dikategorikan dalam kelompok normotensif, preeklampsia, dan preeklampsia dengan sepsis. Sel monosit dinilai pada hitung darah lengkap dengan metode flow cytometry. Kadar IL-6 dan kadar MIF dinilai menggunakan enzyme-linked immunosorbent assay. Hasil penelitian menunjukkan bahwa jumlah sel monosit, kadar IL-6, dan MIF pada kelompok preeklampsia dengan sepsis secara signifikan lebih tinggi dibanding kelompok kontrol (p < 0,001) dan preeklampsia (p < 0,001).  Didapatkan hubungan yang signifikan antara jumlah monosit dengan  kadar IL-6 (r = 0,781; p < 0,001), jumlah monosit dengan kadar MIF (r = 0,798; p < 0,001), dan kadar IL-6 dengan kadar MIF (r = 0,654; p = 0,003)  pada kelompok preeklampsia dengan sepsis. Pada kondisi preeklamsia dengan sepsis, peningkatan jumlah sel monosit dan MIF dapat memicu peningkatan kadar IL-6.
The Effect of Curcumin Administration on p53 and Caspase-3 Expression in Cervical Cancer HeLa Cell Culture Purba, Irene Silvia; Irwanto, Yahya; Rahardjo, Bambang; Handayani, Puspita
Asian Journal of Health Research Vol. 3 No. 3 (2024): Volume 3 No 3 (December) 2024
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v3i3.201

Abstract

Introduction: Cervical cancer is a type of cancer with 605,000 new cases globally each year, with a mortality rate of 340,000. Curcuma longa, or curcumin, has the potential to enhance apoptosis in cervical cancer cells through the NF-kB-p53-caspase-3 pathway. Material and Methods: This in vitro experimental study employed a Completely Randomized Design (CRD) using HeLa cell cultures derived from cervical adenocarcinoma (ATCC® CCL2™). Cells were grown in Dulbecco's Modified Eagle Medium (DMEM) and treated with curcumin at concentrations of 25 µg/mL, 50 µg/mL, 100 µg/mL, 150 µg/mL, and 250 µg/mL. Cytotoxicity was assessed using the CCK-8 assay, apoptosis via flow cytometry, and p53 and caspase-3 expression using immunofluorescence. Statistical analysis was performed with IBM Statistics SPSS 25. Results: There was an increase in the percentage of apoptosis, p53 expression, and caspase-3 expression. At a dose of 100 µg/mL, total apoptosis increased significantly, reaching nearly 25% (p=0.000). P53 expression significantly increased at curcumin doses of 50 µg/mL and 100 µg/mL (p=0.021). There was a significant increase in caspase-3 expression, reaching approximately 86.2%, with a maximum effect at a dose of 100 µg/mL (p=0.002). Conclusion: Curcumin shows promising potential as an anticancer agent by reducing cell viability and enhancing apoptotic activity in HeLa cervical cancer cells.
Effect of Curcumin on Nitric Oxide and Endothelin-1 Levels in L-NAME-Induced Preeclamptic Wistar Rat Rahardjo, Bambang; Dewi, Ratih Frastika; Wiyasa, I Wayan Arsana; Handayani, Puspita; Prasetyorini, Nugrahanti; Wibisono, Hermawan
Majalah Kedokteran Bandung Vol 56, No 4 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3700

Abstract

Preeclampsia is a pregnancy disorder marked by the onset of hypertension after the 20th week, posing risks such as cardiovascular disease. Curcumin, a commonly consumed herbal medicine, has been investigated as a potential antihypertensive agent in mouse models of preeclampsia. This study took place in the Bioscience Laboratory of Universitas Brawijaya from March to August 2023, employing a true experimental design with various groups of mice receiving different treatments. Nitric oxide (NO) and endothelin-1 (ET-1) levels were measured using calorimetry and ELISA. The rats were divided into five groups: positive control, negative control and P1, P2, and P3 as the treatment groups. Treatment grups received fifferent curcumin doses of 30mg/kgBW/day, 50mg/kgBW/day, and 100mg/kgBW/day for P1, P2, and P3, respectively. Data analysis using the One-Way ANOVA and Post Hoc LSD revealed that curcumin at 100mg/kgBW/day significantly increased the NO level of 47.75±22.6 and decreased the ET-1 level of 67.03±24.47 when compared to the positive control (p<0.05). However, the 30mg/kgBW/day and 50mg/kgBW/day doses did not significantly affect the NO and ET levels. In conclusion, curcumin supplementation shows positive effects on NO and ET-1 levels in L-NAME-induced preeclamptic Wistar rats, highlighting its potential as an effective intervention for managing this pregnancy-related disorder.
Effective probiotic regimens for bacterial vaginosis treatment and recurrence prevention: A systematic review Udjianto, Udjianto; Sirat, Noorhamdani A.; Rahardjo, Bambang; Zuhriyah, Lilik
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1671

Abstract

Probiotics represent a promising alternative therapy for bacterial vaginosis; however, consensus on the most effective species, strains, and doses remains lacking, and long-term safety data are limited. The aim of this study was to evaluate the effectiveness of probiotics in managing bacterial vaginosis, considering species, strain, clinical outcomes, optimal dosage, duration, and side effects. This study included randomized-controlled trials (RCTs) published in English (2014–2024) on probiotic treatment for bacterial vaginosis, assessing species, strain, dose, duration, and efficacy. A systematic search was conducted on December 20, 2024, in Scopus, Web of Science, and PubMed using the keywords "vaginosis," "bacterial vaginosis," and "probiotic." Data were extracted and synthesized, and study quality was assessed using the Risk of Bias 2 tool, while NVivo 14 software facilitated thematic analysis. The systematic search yielded 1,560 initial records, of which 16 RCTs were included. The findings revealed that Lactobacillus rhamnosus TOM 22.8 (10×10⁹ CFU/day for 10 days) was the most effective strain and dose, significantly improving Nugent scores, vaginal pH, and microbiota composition and reducing bacterial vaginosis recurrence rate. Alternative strains, including L. crispatus, L. plantarum, and L. acidophilus, showed therapeutic potential at doses of 1×10⁸ to 5.4×10⁹ CFU/day for treatment durations ranging from 6 days to 4 months. The reported side effects were mild and self-limiting. This study supports the use of probiotics as an adjunctive or alternative bacterial vaginosis treatment, emphasizing the need for a personalized approach based on patient characteristics. However, limitations such as small sample sizes and heterogeneous outcome measures necessitate further research. Larger, well-designed trials with standardized methodologies are required to refine probiotic recommendations.
Curcumin reduces inflammation process in mice model preeclampsia Rahardjo, Bambang; Widyalaksono, Agung; Baihaqi, Irfani
Majalah Obstetri & Ginekologi Vol. 33 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V33I12025.21-29

Abstract

HIGHLIGHTS Increased oxidative stress results in increased iNOS in pre-eclampsia. COX-2 expression in the placenta of women suffering from preeclampsia increases and is associated with increased thromboxane production.   ABSTRACT Objective: Preeclampsia is one of the most frequently documented pregnancy complications, with a prevalence of approximately 2 to 15% of all pregnancies. Preeclampsia is a leading cause of maternal mortality. According to research in Indonesia, preeclampsia accounts for 66.8% of all cases of hypertension during pregnancy. This research aims to evaluate the impact of curcumin on serum levels of COX-2 and iNOS in a mouse model of preeclampsia. Materials and Methods: This study employed a true experimental design with a post-test-only controlled group approach using pregnant Rattus norvegicus as a preeclampsia model. Curcumin was administered orally via a feeding tube after dissolving powdered tablets. Dosages were 30 mg/day, 50 mg/day, or 100 mg/day, adjusted for the rats' weight. Serum COX-2 and iNOS levels were measured using ELISA kits from Bioassay Technology Laboratory, with concentrations reported in pg/ml. Analysis was performed using SPSS for Windows 19.0. Results: Serum COX-2 levels showed significant differences (p < 0.05) across groups. L-NAME treatment increased COX-2 levels compared to the negative control. Curcumin (50 and 100 mg/kgBW) reduced COX-2 levels significantly compared to the positive control, with no notable differences between curcumin doses. For iNOS levels, significant differences were also found (p < 0.05). Curcumin at 100 mg/kgBW significantly lowered iNOS levels compared to the positive control, with no significant differences between other treatment groups. Conclusion: Curcumin administration effectively reduces COX-2 and iNOS levels in the serum of Rattus norvegicus with a preeclampsia model.
Co-Authors A.A. Santi Dewi Abd. Rasyid Syamsuri Agustina Tri Endharti Aldiansyah, Dudy Aldika Akbar, Muhammad Ilham Alim, Fathi Nabila Aloysius Suryawan Amir, Nayla Nabilla Tahta Avwina Andonotopo, Wiku Anggie Diniayuningrum Anggraeni, Yessy Mulyanur Anik Sri Purwanti Anin Indriani Anisa Setyowati Anita Dwi Rahmawati Annisa Jusuf, Annisa Annissa Febriani Astuti, Ludji Aziz, Ali Bachnas, Muhammad Adrianes Baihaqi, Irfani Cholid Tri Tjahjono Cut Meurah Yeni Daniati, Lidya Dewi Azar Nuria Wardani Dewi, Ratih Frastika Dinda Oktia Maghfiroh Edy Mustofa Erna Amin Evert Solomon Pangkahila Fauqo Wildatil Jannah Gusti, Kharisma Ciptaning Harumsari, Stefani Hery Setiawan, Hery Hidayat Sujuti Hikmawan Wahyu Sulistomo I Nyoman Hariyasa Sanjaya I Wayan Subage Ihda Dian Kusuma Ihda Dian Kusuma Indrayanti Indrayanti Indriani, Anin Indriati Dwi Rahayu Irfani Baihaqi Ita Sulistiani Julian Dewantiningrum Kalsumy, Umi Kharisma Ciptaning Gusti Kurjak, Asim Kusworini Handono Lestari, Ni Putu Yunita Sri Lilik Zuhriyah Ludji Astuti Melani Chysti Situmorang Mohammad Saifur Rohman Mosip, Erinus Mukhamad Nooryanto Noviyanti Noviyanti, Noviyanti Nugrahanti Prasetyorini Nur Sophia Matin Nurdiana Nurdiana Nurdiana Nurdiana Nurdiana Nurdiana Nuswil Bernolian Pande Made Dwijayasa Permatasari, Galuh W. Pramono, Mochammad Besari Adi Pratama, Harry Dwi Purba, Irene Silvia Purbandari, Rosalia Puspita Handayani Putra, Ichsan Setya Ratnawati, Linda Regina Ayu Fristiyanti Ryan Saktika Mulyana Sabilla Khaerunissa, Dymphna Safrina Dewi Ratnaningrum Sanarto Santoso Sanggelorang, Margie Cassie Setyowati, Anisa Sirat, Noorhamdani A. Siti Candra Windu Baktiyani Siti Candra Windu Baktiyani Situmorang, Melani Chysti Sri Poeranto, Sri Sri Sulistyowati Sri Winarsih Stanojevic, Milan Subandi Subandi Sugiono, Achmad Sulistiani, Ita Sulistomo, Hikmawan Wahyu Sultanah Zahariah Sumarno . Sumarno Sumarno Sutrisno Sutrisno Talita Nandia Primarintan Tatit Nurseta Tatit Nurseta Theresia Monica Rahardjo Udjianto, Udjianto Wardani, Dewi Azar Nuria Wardani, Diadjeng Setya Wardani, Widya Kusuma Wati, Linda Ratna Wibisono, Hermawan Widyalaksono, Agung Winasih, Sri Wiradnyana, Anak Agung Gede Putra Wiyasa, I Wayan Arsana Yahya Irwanto Yogibuana, Valerinna Yuyun Yueniwati Zahariah, Sultanah