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All Journal Jurnal Sain Veteriner Jurnal Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan

Abdul Samik

Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Airlangga Surabaya

Author-ID : 3573552

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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PERSENTASE KEJADIAN KAWIN BERULANG SAPI PERAH YANG TELAH DI IB (INSEMINASI BUATAN) TINGKAT PETERNAK DESA NYAWANG KIDUL DI KUD TANI WILIS KECAMATAN SENDANG KABUPATEN TULUNGAGUNG Arda Wahyu Setyadi; Pudji Srianto; Oky Setyo Widodo; Wurlina Wurlina; Abdul Samik; Tjuk Imam Restiadi
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Dairy cows farm business level of KUD Tani Wilis in Nyawang Kidul, Sendang district, Tulungagung city has some problems resulting low livestock productivity. One of the causes of low levels of dairy reproductivity is the incidence of repeat breeder. Repeat breeder is a condition of a female cow that failed to breed after being mated three or more times with a normal and healthy fertilized bull. The main purpose of this research is to know the risk factor of repeat breeder in dairy cows at farmer level of KUD Tani Wilis in nyawang kidul, Sendang district, Tulungagung city. The type of research is descriptive research. This research was using 32 dairy cows from 6 breeders, the selection of cattles used Simple Random Sampling method. The collection data using questionnaires and direct observations in farms resulted that the evidence rate of dairy cattle's repeat breeder in this research is 45.7%. The results also showed that the influence of knowledge of farmers on disease or reproductive disorders positively correlated with the evidence of repeat breader.

Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling Sarah Azura; Hermin Ratnani; Suherni Susilowati; Mas'ud Hariadi; Abdul Samik; Koesnoto Soepranianondo
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.

Penggunaan kombinasi Gonadotropin untuk pengobatan hipofungsi ovarium pada sapi perah Novia Alfi Masruro; Sri Mulyati; Sri Pantja Madyawati; Abdul Samik; Hermin Ratnani; Nenny Harijani
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This case report aims to determine the response of estrus and pregnancy rates in dairy cows with ovarian hypofunction by administering a combination of gonadotropin hormones. The sample used was ten dairy cows that had been calving, were not pregnant, showed no signs of estrus for more than 60 days after the last calving and had ovarian hypofunction based on rectal palpation. All dairy cows were injected 300 IU PG-600, then divided into two groups, each injected with 100 and 300 IU hCG respectively for first group and second group, intramuscularly at the time of insemination. The results showed that the estrus response occurred in all cows, in 6-8 (6.8 ± 0.84) days (first group), and 6-7 (6.2 ± 0.45) days (second group). Trans-rectal palpation pregnancy diagnosis 60 days after artificial insemination showed that all dairy cows in both groups were pregnant. It could be concluded that the treatment of dairy cows suffering from ovarian hypofunction with a combination of 300 IU PG-600 and 100 IU hCG was sufficient to induce 100% estrus rates and 100% pregnancy rates.

Penambahan alfa-tokoferol dalam pengencer susu skim - kuning telur terhadap kualitas spermatozoa domba Sapudi yang disimpan pada suhu 5°C Satya Alysa Cahya Puspita; Suherni Susilowati; Trilas Sardjito; Abdul Samik; Indah Norma Triana; Soeharsono Soeharsono
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.69-76

Spermatozoa in fresh semen of Sapudi ram has a limited life span. The storage of semen in cold temperatures (5 °C) is intended to prolong the spermatozoa's life. However, storage in cold temperatures can lead to increased production of reactive oxygen species (ROS). This condition reduces the quality of spermatozoa. The purpose of this study was to determine the effect of alphatocopherol supplementation in skim milk-egg yolk extender on viability, motility, and plasma membrane integrity of Sapudi ram spermatozoa. Fresh semen derived from Sapudi ram was divided into four treatment groups. Control treatment (P0): semen was added in the extender of skim milkegg yolk without alpha-tocopherol. Three other treatments: P1, P2, and P3 semen were added in skim milk-egg yolk extender with the supplementation of 0.25, 0.5, and 1 gram alpha-tocopherol/ 100 mL extender, respectively. The results showed that the viability, motility, and integrity of the spermatozoa plasma membrane decreased gradually according to the storage length. Supplementation of skim milk-egg yolk extender with 0.5 gram of alpha-tocopherol/100 mL (P2) was able to maintain spermatozoa quality longer (p <0.05) than the control group. It can be concluded that alpha-tocopherol with a concentration of 0.5 g/100 mL of skim milk-egg yolk extender effectively maintains the quality of Sapudi ram spermatozoa in storage at 5 ° C.

Relationship between the value of the estrous detector measurement result and serum progesterone level in Etawa crossbred goats after estrous synchronization Annisa Lita Triagil; Ismudiono Ismudiono; Tita Damayanti Lestari; Abdul Samik; Budi Utomo; Boedi Setiawan
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.82-85

This study was conducted to determine the relationship between the estrous detector's reading value (Draminski®, Dramiński S.A, Poland) and serum progesterone levels on Etawa crossbred does. Eighteen healthynon-prehnant Etawa crossbreds does weighing 20-25 kg, in their first parity were used in this study. The does were divided equally into two groups. In the two groups, the does were synchronized their estrus by means of intramuscular and intravulvar injection of 7.5 mg prostaglandin Fɑ (PGF2ɑ) twice in 11-day intervals. After the second injection, the estrous detector value measurement, visual observation of the estrus sign, and blood sampling were conducted for five days. The blood sample was processed into the serum to examine for progesterone levels by ELISA. The data analysis was using the simple correlation method on SPSS type 23 for windows. The result showed that a positive relationship (p <0.05) with coefficient correlation (r) was 0.609 between the values of the estrous detector measurement with serum progesterone levels. It could be concluded that the estrous detector's low value indicates that the Etawa crossbred was in the estrus phase and followed by the decreasing of progesterone level but not reaching the basal level.

Polip endometrium pada sapi bunting tua Anang Yusuf; Thomas Valentinus Widiyatno; Abdul Samik
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.25-31

The current report describes acute death in 8.5 months pregnant cow, which no signs of illness before. Postmortem examination showed the presence of gastric bloat. Necropsy revealed no change in the viscera, but yellow and cloudy nodules were found on the endometrial wall, amniotic fluid mixed with blood, blood clots in the inner cervical canal, pneumonia in the lungs. Meanwhile, the anatomical condition of the fetus was normal. It could be concluded that the cow's sudden death was due to late pregnancy, causing high tension that triggers rupturing of the polyp blood vessels. The deep vein thrombosis due to late pregnancy causes pulmonary emboli and subsequent pneumonia.

Correlation of parity and age to services per conception, conception rate, and gestation length in Holstein Friesian cross cows Norma Amalia Ulfah; Abdul Samik; Mas’ud Hariadi; Tri Wahyu Suprayogi; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.9-14

Small farmers dominate dairy farming in Indonesia, and the average productivity is low. The contribution of domestic production only reaches about 23% of the total national milk consumption needs as a source of human nutrition. This study was aimed to identify the relationship between cow's parity and age to services per conception (S/C), conception rate (CR), and gestation length (GL) of Holstein Friesian (HF) cross dairy cows. Data of this study consisted of primary data obtained from farmers interview, and secondary data from the Cooperative Unit of Argopuro village, Krucil, Probolinggo. Sample size was 200 cows, which was 10% of the total population. Data were analyzed for correlation and linear regression using the Statistic Package and Service Solution (SPSS) software version 20 (IBM Corp., USA) at a 95% confidence level. The average S/C, CR, and GL were 2.55 ± 1.57, 21%, and 278.19 ± 11.92 days, respectively. There was no significant correlation (p >0.05) between the parity with S/C, CR, and GL and the age with CR and GL. The only significant correlation (p <0.05) was between the age and S/C. Regression of S/C based on age followed the equation S/C = 0.104 + 0.407*age, with a correlation coefficient and determination coefficient of 0.733 and 0.538, respectively. It could be concluded that S/C could be predicted based on the age of the cows.

pH and ferning score of cervical mucus related to gestation and calf gender of Holstein Friesian crossbreed cows Muhammad Billy Ferdiansyah; Iwan Sahrial Hamid; Herry Agus Hermadi; Abdul Samik; Tatik Hernawati
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.22-26

This study aimed to compare the ferning score based on the pH of the cervical mucus and its relation with gestation and the calf gender of cows. Ten Holstein Friesian crossbreed cows which were detected in estrus and about to be artificially inseminated, were used for this study. Sample of cervical mucus was collected using Metricheck for pH and ferning observations. Pregnancy diagnosis was conducted two months after insemination, and the gender of the calves was recorded at parturition. This study indicated that cervical mucus pH did not affect the ferning characteristics, ferning score, and pregnancy of cows. However, higher ferning scores tended to result in the pregnancy of cows. Also, alkaline pH tended to result in male calves and vice versa. Further study is needed with a more significant number of samples related to the application of ferning display as an indicator of estrus to obtain a higher pregnancy rate.

Cranium morphometry for distinguishing male and female Muscovy duck (Cairina moschata) before sexual maturity Mafatichul Firdaus; Abdul Samik; Yeni Dhamayanti; Raden Tatang Santanu Adikara; Hana Eliyani; Soeharsono Soeharsono
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 2 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i2.2022.54-58

Gender determination of Muscovy duck as early as possible is essential for the efficiency and effectiveness of duck production. This study aimed to determine the morphometric differences in prepubertal male and female Muscovy duck (Cairina moschata) as predictors of gender. This study used a split-plot design, with the observed variables cranium length, width and height, mandibular length, and rostrum length and width. This study used a total of 80 samples of duck heads consisting of 40 male and 40 female Muscovy duck heads, with ten heads each in the age group of 2, 4, 6, and 8 weeks, respectively. Results showed that the length, width, and height of the cranium, mandibular length, and rostrum lengthand width were greater in males (p <0.05) than those of the females in all age groups. It could be concluded that the morphometric size of prepubertal male Muscovy ducks head was greater than those of the female ones at the same age; thereby, it could be used to distinguish gender. Based on the morphometrics, further study can also be conducted to measure other body parts of the Muscovy duck apart from the cranium. In addition, it is necessary to study the morphometric measurements of the cranium in other poultry as a basis for identifying sex.

Characterization of Pregnancy Specific Protein B (PSPB) from Placenta Foetalis (Cotyledon) Friesian Holstein Cows Abdul Samik
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

This research was aimed to find out a substance that was useful in early pregnancy diagnosis of Friesian Holstein cows. Pregnancy Specific Protein B (PSPB) was isolated, purified and partially characterized from cotyledon Friesian Holstein cows. Characterization of PSPB protein was conducted using SDS-PAGE and Western Blot. Antisera were developed against PSPB and by immunohistochemical techniques the protein was localized to the binucleated cells of the cotyledon. The estimated molecular size of Friesian Holstein cows PSPB was 59.88 kDa with the concentration of PSPB protein in cotyledon was 6480 ng/ml. PSPB protein could induce anti-PSPB antibody with the value of optical density (OD) were 0.179±0.0102 (before immunization); 1.466±0.3288 (3rd week after immunization); 1.936±0.4754 (1st week after booster;) and 2.256±0.4842 (2nd week after booster).

Co-Authors Adikara, Tatang Santanu Afiff , Usamah Anang Yusuf Annisa Lita Triagil Anriansyah Renggaman Arda Wahyu Setyadi Bimo Aksono Boedi Setiawan BUDI UTOMO Dea Indriani Astuti Dedi Rahmat Setiadi Eka Pramyrtha Hestianah Fierda Kabayo Hana Eliyani Hani Plumeriastuti Hanifah Muslimah Ananda Hermin Ratnani Herry Agoes Hermadi Herry Agus Hermadi Imam Mustofa Indah Norma Triana Intan Purwa Dewanti Ira Sari Yudaniayanti Ismudiono Ismudiono Iwan Sahrial Hamid Koesnoto Soepranianondo Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Mafatichul Firdaus Mas'ud Hariadi Mas’ud Hariadi Mas’ud Hariadi Mufasirin MUHAMMAD AGIL Muhammad Billy Ferdiansyah Muhammad Thohawi Elziyad Purnama Nadia Yohana Nenny Harijani Ngakan Made Rai Widjaja Norma Amalia Ulfah Nove Hidajati Novia Alfi Masruro Poedji Hastutiek Prastiya, Ragil Angga Pudji Srianto R.T.S Adikara R.T.S Adikara Ricko Nurdianto S. Saputro, Amung Logam Sarah Azura Satya Alysa Cahya Puspita Soeharsono Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Hidanah Sri Mulyati Sri Pantja Madyawati Suherni Susilowati Surya Agus Prihatno Suzanita Utama Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Warda Nafalizza Efendi Widiyatno, Thomas Valentinus Widodo, Oky Setyo Wurlina, W Yeni Dhamayanti Yosua Kristian Adi Yuanara Augusta Rahmat Adikara Yudit Oktanella, Yudit

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