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RESPONS SEL EPITEL USUS (CMT-93) TERHADAP NUTRASETIKAL GALOHGOR Katrin Roosita; Rimbawan R; Ita Djuwita; M. Rizal Damanik; Clara M. Kusharto
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.665 KB) | DOI: 10.21157/j.ked.hewan.v9i2.2825

Abstract

Penelitian ini bertujuan membandingkan pengaruh nutrasetikal galohgor dalam bentuk serbuk (GS) dan ekstrak (GE) terhadap proliferasi, morfologi, dan ekspresi gen Aldh1a2 pada sel epitel usus (CMT-93). Galohgor serbuk (GS) dibuat dengan menghancurkan semua bahan dandikeringkan menggunakan drum-dryer sedangkan GE dibuat dengan mengeringkan semua bahan dengan oven, digiling, dan dimaserasi dengan etanol selama 3x24 jam. Perlakuan didasarkan pada konsentrasi akhir β-karoten yang berasal dari GS dan GE masing-masing sebesar 0,5; 1,5; dan 5,0 µM dalam larutan medium Dulbecco's Modified Eagle's medium (DMEM) yang dilengkapi serum (10%). Analisis proliferasimenggunakan asai 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dan ekspresi gen dianalisis dengan reverse transcriptasepolymerase chain reaction (RT-PCR). Hasil penelitian meunjukkan bahwa GE pada konsentrasi tinggi (5,0 μM) secara signifikan (P0,05) dapatmenekan proliferasi dan memengaruhi morfologi sel CMT-93. Beta-karoten dalam GE dan GS memengaruhi ekspresi gen Aldh1a2 pada sel epitel usus CMT-93
Formulasi Cookies Sagu, Ganyong, Dan Galohgor Sebagai Alternatif Kudapan Bagi Penderita Diabetes Melitus Tipe II Aulia Ratnadianti Suharjo; Katrin Roosita
Indonesian Journal of Human Nutrition Vol. 6 No. 2 (2019)
Publisher : Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijhn.2019.006.02.3

Abstract

Abstrak Penelitian ini bertujuan untuk mengembangkan produk cookies sagu, ganyong, dan galohgor sebagai kudapan alternatif bagi penderita diabetes melitus. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan empat taraf formula, yaitu F0 (kontrol), F1 (12.5% tepung ganyong, 0% ekstrak jamu galohgor), F2 (10% tepung ganyong, 2.5% ekstrak jamu galohgor), dan F3 (0% tepung ganyong, 2.5% ekstrak jamu galohgor). Cookies sagu, ganyong, dan galohgor memiliki tingkat penerimaan mencapai 82%. Kandungan zat gizi cookies sagu, ganyong, dan galohgor adalah 5.3% (b/b), abu 2.4% (b/k), lemak 26.8% (b/k), protein 2.4% (b/k), serat kasar 3.1% (b/k), dan karbohidrat 63.1% (b/k). Total flavonoid pada cookies sagu, ganyong, dan galohgor setara dengan 22 mg quercetin equivalen/100 gram. Cookies ganyong galohgor dalam satu kali sajian (40 gram) dapat memenuhi kebutuhan energi dan zat gizi wanita berusia 50-64 tahun sebagai berikut: 12.9% energi, 1.5% protein, 30.5% lemak, 4.9% serat, dan 10.2% karbohidrat.Kata kunci: diabetes melitus, cookies, ganyong, galohgor Abstract Preconception women who have anemia are at risk of suffering problems during pregnancy. One of risk The aim of this study was to develop sago, ganyong, and galohgor cookies as a healthy snack for diabetes mellitus patients. Study design was Complete Randomized Design. This study using four formula levels F0 (control), F1 (12.5% ganyong flour, 0% galohgor extract), F2 (10% ganyong flour, 2.5% galohgor extract), and F3 (0% ganyong flour, 2.5% galohgor extract). Sago, ganyong, and galohgor cookies had 82% acceptance level. Nutrient contents and characteristics of sago, ganyong, and galohgor cookies were as follows 5.3% (w/w) moisture,  2.4% (d/w) ash, 26.8% (d/w) fat, 2.4% (d/w) protein, 3.1% (d/w) crude fiber, and 63.1% (d/w) carbohydrate. The flavonoid value of sago, ganyong, and galohgor cookies was equal to 22 mg quercetin equivalent/100 g. Sago, ganyong, and galohgor in one serving size (40 grams) might contribute to energy and nutrients requirment of woman aged 50-64 years, as much as  follows: 12.9% energy, 1.5% protein, 30.5% fat, 4.9% fiber and 10.2% carbohydrate. Keywords: diabetes mellitus, cookies, ganyong, galohgor
RESPONS SEL EPITEL USUS (CMT-93) TERHADAP NUTRASETIKAL GALOHGOR Katrin Roosita; Rimbawan R; Ita Djuwita; M. Rizal Damanik; Clara M. Kusharto
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2825

Abstract

Penelitian ini bertujuan membandingkan pengaruh nutrasetikal galohgor dalam bentuk serbuk (GS) dan ekstrak (GE) terhadap proliferasi, morfologi, dan ekspresi gen Aldh1a2 pada sel epitel usus (CMT-93). Galohgor serbuk (GS) dibuat dengan menghancurkan semua bahan dandikeringkan menggunakan drum-dryer sedangkan GE dibuat dengan mengeringkan semua bahan dengan oven, digiling, dan dimaserasi dengan etanol selama 3x24 jam. Perlakuan didasarkan pada konsentrasi akhir β-karoten yang berasal dari GS dan GE masing-masing sebesar 0,5; 1,5; dan 5,0 µM dalam larutan medium Dulbecco's Modified Eagle's medium (DMEM) yang dilengkapi serum (10%). Analisis proliferasimenggunakan asai 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dan ekspresi gen dianalisis dengan reverse transcriptasepolymerase chain reaction (RT-PCR). Hasil penelitian meunjukkan bahwa GE pada konsentrasi tinggi (5,0 μM) secara signifikan (P0,05) dapatmenekan proliferasi dan memengaruhi morfologi sel CMT-93. Beta-karoten dalam GE dan GS memengaruhi ekspresi gen Aldh1a2 pada sel epitel usus CMT-93
The influences of nutritional status and abdominal circumference systolic blood pressure in adult men Ni'ma Hilyatin; Katrin Roosita; Rimbawan Rimbawan; Mohamad Rafi
AcTion: Aceh Nutrition Journal Vol 9, No 2 (2024): June
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v9i2.1668

Abstract

The prevention and management of hypertension through healthy lifestyle modifications is a challenge for public health. The prevalence of hypertension among Indonesians was 34,1%. Lifestyle behaviors such as body mass index, central obesity, and sodium intake are associated with the risk of hypertension. This study aimed to analyze the influence of nutritional status and abdominal circumference on blood pressure in adult men. This cross-sectional research was conducted in Sukajadi Village, Bogor, between 2022-2023. The subjects were 73 purposively selected adult men. Data on subject characteristics were obtained through interviews. Anthropometric data were obtained by measuring abdominal circumference, body weight, and body height to assess the body mass index (BMI). Consumption data were collected using SQ-FFQ. The data analysis was performed using a linear regression test with a significance level of 95%. The results showed that Body mass index (p=0,007) and abdominal circumference (p=0,005) affected systolic blood pressure, while sodium intake (p=0,906) was not related to systolic blood pressure in adult men. Conclusions: Body mass index and abdominal circumference greatly influenced the systolic blood pressure. Further studies are needed to determine the various factors that influence systolic blood pressure and the risk of hypertension.
Polymorphism of PPM1K Gene and The Association Related to Retail Indonesian Sheep Meat Cuts Suhendra, Muhammad; Ratna Sholatia Harahap; Kasita Listyarini; Cece Sumantri; Cahyo Budiman; Katrin Roosita; Gunawan, Asep
ANIMAL PRODUCTION Vol. 26 No. 1 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.1.216-1

Abstract

The PPM1K (Protein Phosphatase, Mg2+/Mn2+ Dependent 1K) gene is assumed to associate with retail meat cuts. This study aims to determine the association of the PPM1K gene polymorphisms with retail meat cuts among various breeds of Indonesian sheep. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) approach was used to determine the polymorphisms of the PPM1K gene in 130 Indonesian rams aged 10-12 months with body weights ranging from 20-25 kg. The association study between PPM1K and retail meat cuts was analyzed using General Linear Model (GLM). The research showed that the PPM1K gene was polymorphic, with three genotypes: AA (458 bp), GG (259 bp and 199 bp), and AG (458 bp, 259 bp, and 199 bp). The PPM1K gene polymorphisms were significantly (P<0.05) associated with breast cut, which is the breast muscle and bone. The GG genotype of the PPM1K gene had a higher value on breast lamb. This result implies that the PPM1K gene could be a potential candidate for marker-assisted selection for improving the lamb quality of Indonesian sheep.
Analysis of Amino Acid Profiles in Bone, Oil, and Muscle Tissue and Wound Healing Activity of Pangasius hypophthalmus Oil in Rattus norvegicus Hidayaturrahmah, Hidayaturrahmah; Suprayogi, Agik; Hanif, Novriyandi; Roosita, Katrin; Darusman, Huda Shalahudin; Aprilyana, Lia
Borneo Journal of Pharmacy Vol. 8 No. 1 (2025): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v8i1.8182

Abstract

Patin (Pangasius hypophthalmus), a commercially important freshwater fish, is a rich source of protein containing both essential and non-essential amino acids, crucial for various biological functions. This study aimed to determine the amino acid composition of muscle tissue, oil, and bones of P. hypophthalmus and evaluate the wound healing potential of its oil extract in Rattus norvegicus. High-Performance Liquid Chromatography was employed to quantify the levels of essential and non-essential amino acids in each tissue. Results revealed that P. hypophthalmus oil exhibited the highest total amino acid content, particularly rich in histidine, arginine, and lysine. The wound healing activity of P. hypophthalmus oil extract was assessed in a rodent model, demonstrating a significant reduction in wound area (0.62-0.84 mm) and an impressive wound healing percentage (79-84.45%). These findings suggest that different parts of P. hypophthalmus possess valuable nutritional and therapeutic properties. Notably, the high amino acid content of P. hypophthalmus oil, particularly essential amino acids, highlights its potential as a promising source for developing pharmaceutical products, including amino acid supplements, wound healing agents, and formulations for metabolic support. Further research is warranted to fully explore the therapeutic potential of P. hypophthalmus oil and its bioactive components.
KECUKUPAN VITAMIN A DAN PRAKTEK PEMBERIAN AIR SUSU IBU SERTA KELENGKAPAN IMUNISASI DASAR DAN MORBIDITAS BAYI Mastin, Masruroh; Roosita, Katrin
Jurnal Gizi dan Pangan Vol. 10 No. 1 (2015)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.625 KB) | DOI: 10.25182/jgp.2015.10.1.%p

Abstract

ABSTRACTThe study was aimed to analyze correlation of maternal vitamin A sufficiency, exclusive breastfeeding practices and basic immunization with infant morbidity. The cross sectional study was conducted in four village at Gurah Subdistrict, Kediri District and 31 subjects were included. The data of maternal’s food consumption, exclusive breastfeeding practices, basic immunization and infant morbidity were collected by interview and the infant nutritional status was collected by measuring body weight and length. Maternal vitamin A sufficiency were 58% sufficient and 42% insufficient. There was 68% of infants suffered of diseases, i.e. respiratory disease, fever, diarrhea. Higher maternal vitamin A sufficiency corellated with shorter period of infant’s illnesses. Meanwhile, exclusive breastfeeding practices associated with period and lower frequency of illnesses.Keywords: infant, infectious disease, lactating woman, morbidity, vitamin AABSTRAKPenelitian ini bertujuan untuk mengetahui keterkaitan kecukupan vitamin A ibu, praktek pemberian air susu ibu (ASI) eksklusif dan kelengkapan imunisasi dengan kejadian penyakit infeksi pada bayi usia 5-6 bulan. Penelitian cross sectional ini dilakukan di empat desa di Kecamatan Gurah, Kabupaten Kediri dengan jumlah subjek 31 orang. Data diambil dengan pengukuran dan wawancara secara langsung. Data yang digunakan meliputi konsumsi pangan ibu, praktek pemberian ASI eksklusif, kelengkapan imunisasi dan morbiditas bayi. Hasil penelitian menunjukkan bahwa kecukupan vitamin A ibu termasuk dalam kategori cukup (58%) dan kurang (42%). Sebanyak 68% bayi pernah mengalami sakit selama satu bulan terakhir. Jenis penyakit yang diderita oleh bayi selama sebulan terakhir adalah infeksi saluran pernapasan atas (ISPA), demam, dan diare. Semakin baik kecukupan vitamin A ibu, maka periode lama sakit bayi cenderung semakin singkat. Sementara itu, praktek pemberian ASI eksklusif pada bayi cenderung menurunkan frekuensi sakit dan mempersingkat periode lama sakit bayi.Kata kunci: bayi, ibu menyusui, morbiditas, penyakit infeksi, vitamin A
GAYA HIDUP DAN KEJADIAN SINDROM METABOLIK PADA KARYAWAN LAKI-LAKI BERSTATUS GIZI OBES DI PT. INDOCEMENT CITEUREUP Nurjanah, Fitria; Roosita, Katrin
Jurnal Gizi dan Pangan Vol. 10 No. 1 (2015)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.116 KB) | DOI: 10.25182/jgp.2015.10.1.%p

Abstract

ABSTRACTThe objectives of this study were to analyze life style and incidence of metabolic syndrome among obese male employees in PT. Indocement Citeureup. The cross sectional design was applied with purposive sampling by involving 59 obese male employees met the inclusion criteria. There was 49.2% subject has metabolic syndrome whereas the highest prevalence was central obesity (96.6%), followed by hipertriglyceridemia (82.8%), low level of High Density Lipoprotein (HDL) (72.4%), fasting blood glucose (62.1%), and hypertension (55.2%). There was no significant difference prevalence of metabolic syndrome by age, history of obesity, smoking habits, excercise habits, physical activity, and eating behavior (p>0.05). High cigarettes smoking significantly correlated with lower diastole and greater abdominal circumference (p<0.05). Unhealthy eating behavior was significantly correlated with HDL cholesterol, while snacking behavior was correlated with fasting blood glucose (p<0.05).Keywords: life style, metabolic syndrome, obesityABSTRAKTujuan penelitian ini adalah menganalisis gaya hidup dan kejadian sindrom metabolik pada karyawan laki-laki berstatus gizi obes di PT. Indocement Citeureup. Desain penelitian adalah cross sectional dengan purposive sampling dan melibatkan 59 karyawan laki-laki obes yang dipilih berdasarkan kriteria inklusi. Sebanyak 49,15% subjek mengalami sindrom metabolik dengan prevalensi penanda sindrom metabolik tertinggi adalah obesitas sentral (96,55%), diikuti oleh hipertrigliseridemia (82,76%), kadar kolesterol High Density Lipoprotein (HDL) rendah (72,41%), kadar glukosa darah puasa tinggi (62,07%) dan hipertensi (55,17%). Tidak terdapat perbedaan proporsi kejadian sindrom metabolik menurut umur, riwayat kegemukan, kebiasaan merokok, kebiasaan olahraga, tingkat aktivitas fisik maupun perilaku makan (p>0,05). Semakin banyak jumlah rokok yang dikonsumsi berkorelasi signifikan dengan tekanan darah diastol yang rendah dan ukuran lingkar perut yang besar (p<0,05). Perilaku makan yang tidak sehat berkorelasi signifikan dengan rendahnya kadar kolesterol HDL, sedangkan perilaku konsumsi cemilan berkorelasi dengan tingginya kadar glukosa darah puasa (p<0,05).Kata kunci: gaya hidup, obesitas, sindrom metabolik
Identification of AHSG gene polymorphism and association with flavor and odor compounds of Indonesian lamb meat Septiyawan, Ganda Adi; Listyarini, Kasita; Noor, Ronny Rachman; Roosita, Katrin; Gunawan, Asep
Buletin Peternakan Vol 49, No 1 (2025): BULETIN PETERNAKAN VOL. 49 (1) FEBRUARY 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i1.89123

Abstract

Flavor and odor are important factors for consumers to consider the quality of lamb meat. The AHSG Alpha2-Heremans- Schmid Glycoprotein gene is associated with a fatty acid content and is indicated as a gene controlling flavor and odor compounds. The research aims to identify the AHSG gene polymorphism and its association with flavor and odor compounds in Indonesian lamb meat. The sample used in this study was 105 ram samples consisting of 10 Javanese Fat-Tailed Sheep (JFTS), 80 Javanese Thin-Tailed Sheep (JTTS), and 15 Jonggol sheep (JS). The AHSG gene polymorphism was validated using the PCR-RFLP technique with the EagI enzyme EagI. The association of the AHSG gene SNP (g.198655287 G>A) with odor and flavor was analyzed using the T-tests. The AHSG gene polymorphism in Indonesian lamb was polymorphic with three genotypes, i.e., GG, GA, and AA, and in the Hardy-Weinberg equilibrium. The AHSG significance was associated (P<0.05) with skatole compounds (MOA, EOA, MNA, and MI). The AA genotype is recommended for a candidate marker of flavors and odors because it associated with low compound values. The AHSG gene, specifically the SNP g.198655287 G>A, can potentially serve as a genetic marker for lamb meat production by reducing flavor and odor.
The role of stimulation, affection, and parenting patterns in stunting among children aged 24-59 months Yulianti Wulan Sari; Ali Khomsan; Katrin Roosita
AcTion: Aceh Nutrition Journal Vol 10, No 1 (2025): March
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v10i1.2289

Abstract

Stunting remains a chronic nutritional issue in Indonesia. The prevalence of stunting has decreased from 30,8% in 2018 to 21,5% in 2023; this rate is still above the WHO threshold of less than 20%. Proper stimulation, affection, and parenting patterns influenced optimal growth in terms of height and weight. This study aimed to analyze the association between stimulation, affection, and parenting practices in stunting among children aged 24–59 months. This case-control study was conducted from March to April 2024 at the Made Health Center, Surabaya City, which consists of 50 toddlers, both stunting and non-stunting, selected using purposive sampling. Stimulation patterns were measured using the Home Observation of Measurement and Environment Inventory (HOME), affection patterns using the Child Adult Relational Experimental (CARE), parenting patterns using the parenting feeding style questionnaire, and nutritional status using anthropometric measurements. Data analysis was performed using the chi-squared test. The results showed that the stimulation patterns were significantly correlated with stunting (p=0,000; OR= 5,90). Affection patterns were also significantly correlated with stunting (P =0,000; OR=13,75). Parenting patterns were significantly correlated with stunting (P =0,000; OR=26,35). In conclusion, stimulation, affection, and parenting patterns were significantly correlated with stunting among children aged 24-59 months.