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Journal : Jurnal Kesehatan

Pemberian Fraksi Daun Kemangi (Ocimum americanum L.) terhadap Spermatozoa Tikus Putih Jantan (Rattus norvegicus) Vinnata, Nora Novia; Salni, Salni; Nita, Sri
Jurnal Kesehatan Vol 9, No 3 (2018): Jurnal Kesehatan
Publisher : Politeknik Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (898.595 KB) | DOI: 10.26630/jk.v9i3.1021

Abstract

The major cause of a spermatogenetic process disorder in testis is free radical. One of the traditional medicines commonly used as an aphrodisiac is Kemangi plant. Rosary Basil leaf (Ocimum americanum L.), Indonesian name is Kemangi, contains active substances, such as flavonoid, saponin, tannin, and triterpenoid/steroid which is antioxidant and fertility. The purpose of the present research was to analyze the effect of testis weight, diameter, epithelium thickness, morphology, and spermatozoa amount of male white mouse (Rattus norvegicus) after feeding fractions of Kemangi leaf (Ocimum americanum L.) This study was experimental with a posttest-only control group design. The research sample size was 24 furrow male white mice Sprague Dawley, 60-74 days old. They were divided into 4 treatment groups, each being fed the fractions of n-hexane, ethyl acetate, and water-ethanol of Kemangi leaf at a dosage of 100 mg/mouse/day, compared to CMC 1% for the control group for 36 days, and then a dissection was done to take out secreted substances and to examine the histology of testis. The result showed that there were increases of testis weight in the treatment group as compared to that of the control (p=0.018). In addition, there were increases in diameter and thickness of seminiferous tubule epithelium of testis, in the morphology of abnormal sperm (p=0.000), and in spermatozoa amount (p=0.020). Fractions of Kemangi leaf can increase testis weight, diameter, and epithelium thickness, morphology, and spermatozoa amount of furrow male white mice Sprague Dawley, particularly the fraction of ethyl acetate. 
Pengaruh Pemberian Fraksi Daun Kemangi (Ocimum americanum L.) terhadap Berat, Diameter, Tebal Epitel Epididimis, Motilitas dan Viabilitas Spermatozoa Tikus Putih Jantan (Rattus norvegicus) Louis, Stephanie Lexy; Salni, Salni; Nita, Sri
Jurnal Kesehatan Vol 10, No 1 (2019): Jurnal Kesehatan
Publisher : Politeknik Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.846 KB) | DOI: 10.26630/jk.v10i1.1079

Abstract

Infertility is one of the reproductive problems that may affect both male and female. In a male, the occurrence of reproductive problems may be due to abnormal spermatogenesis processes and anatomic disorder. Kemangi leaves are an aphrodisiac it contains some secondary metabolites like flavonoid, steroid, tannin, and saponin. The objective of the present research was to determine the effect of feeding fractions of kemangi leaves (Ocimum americanum L.) on the epididymis weight, diameter, and epithelial thickness and the spermatozoa motility and viability of white male rat (Rattus norvegicus). The research was an experimental one, with posttest only group design. The research design used a Completely Random Design. The research sample consisted of 24 furrow white male mice Sprague Dawley, 60-74 days old, divided to control group, a fraction of n-hexane, a fraction of ethyl acetate, and a fraction of water-ethanol. The dosage used for the treatment group was 100 mg/kgBB/day, and the control group was given CMC 1%. The result of data analysis by using a One-Way Anova test showed that there were increases in the weight and diameter of the epididymis in the treatment group as compared to that of the control group (p=0,000). Likewise, both epididymis epithelium thickness and spermatozoa viability in the treatment group increased as compared to that of the control group (p=0.001), while spermatozoa motility was p=0,002. It could be concluded that the fractions of kemangi leaves increased the weight, diameter, and epithelium thickness of epididymis and spermatozoa motility and viability in-furrow white male rat Sprague Dawley.