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Journal : BIOVALENTIA: Biological Research Journal

THE ANTIBACTERIAL ACTIVITY OF WATER APPLE LEAVES ACTIVE COMPOUND (Syzygium zeylanicum) AGAINST Escherichia coli AND Staphylococcus aureus Hamidah, -; Salni, -; Tanzerina, Nina
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.804 KB) | DOI: 10.24233/BIOV.3.1.2017.54

Abstract

Escherichia coli is one of the bacteria that cause infections in the human digestive tract such as diarrhea, while Staphylococcus aureus is one of the bacteria that cause infections in the skin injury such as boils and pimples. This study used Syzygium zeylanicum leaves because it has potential as a antibacterial because it contains active compounds. This study aimed was determine the antibacterial activity of the fraction and the active compound in Syzygium zeylanicum leaves against Escherichia coli and Staphylococcus aureus. Research conducted on November 2015 to January 2016. The method used in this research were extraction by maceration, fractionation by liquid fractionation, antibacterial activity test, and determination of minimum inhibitory concentration with the diffusion method and isolation of active compounds by column chromatography method. The bacteria used in this test are Escherichia coli and Staphylococcus aureus. Data are presented in tabular form based on the average value of the inhibition diameter and deviation standard. The results of this research showed the water methanol active fraction against the bacteria that used in this test. The methanol water fraction had obtained one antibacterial compound in bottle 1,3,5 which shows the value of tannin Rf 0,416. The minimum inhibitory concentration of water methanol of water apple leaves is 1000 µg/mL for Escherichia coli and 500 µg/mL for  Staphylococcus aureus. The minimum  inhibitory concentration of the active  compound  to  Escherichia  coli  and  Staphylococcus  aureus  in  500  µg/mL.  The fraction and the active compound of water apple leaves have an antibacterial activity with Escherichia coli and Staphylococcus aureus and the active compound is tannin.
EVALUATION OF LOCAL RECLAMATION DATA FOR SUSTAINIBILITY INFRASTUCTURE FACILITIES (CASE STUDY OF KLAWAS ZONE AND SUBAN ZONE IN POST COAL MINING AIR LAYU TANJUNG ENIM) Gumanti, Johnnedy; Sutriyono, Edy; Salni, Salni; Harnani, Harnani
BIOVALENTIA: Biological Research Journal Vol. 4 No. 1 (2018)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (764.813 KB) | DOI: 10.24233/BIOV.4.1.2018.109

Abstract

This study identifies and analyzes the biophysical and environmental aspects of the environment, potentials and constraints, and then evaluates the post-mining reclamation land use that will be used for Other Use Areas (APL), the Residential Infrastructure Facility. Based on the Mining Closure Plan (RPT) on the utilization of former coal mine land at Air Laya Tanjung Enim, Klawas reclamation land use plan for Resettlement Facility and Zoning Infrastructures (Zone 2) while Suban reclamation land use plan for Productive Research area. Analysis in this research are scoring, SWOT and map overlay such as area function analysis and land suitability analysis based on physical condition of existing land. The result of this research is land suitability level for Residential Infrastructure Facility in Klawas, suitable land equal to 12% or 38 ha while 98% unfit or 258,7 ha from total area of 277,8 ha. Terms of land suitability in Klawas research area including Marginal (S3) with total scores of 80. where climate condition support with average temperature 24oC and rainfall 23,93 - 27,87 mm / day, slope of flat slope approaching water level river 0 - 3%, complex geological structure there are anticline in the form of fault and fracture, soil type Alluvial and Latosol, there are many aquifer with shallow depth to medium, there is vulnerability of geological disaster in the form of mass movement of soil / rock, flood, erosion and radiation exposure TENORM of moderate intensity. The results of land use evaluation for the Klawas reclamation area are more suitable for Freshwater Fishery Cultivation area than for Residential Infrastructure Facility area. Land tenure rate for Residential Infrastructure Facility in Suban, suitable land equal to 76 % or 237 ha while unsuitable 24% or 105 ha of total area of 342 ha. Conditions of land suitability in Suban's research area include Match (S2) with a total scores of 130. Climatic conditions support the average air temperature of 24°C and rainfall of 23.93 - 27.87 mm / day, the slope of flat slope is 3 - 8% there is no anticline structure in the form of fault, Andosol and Podsolic soil types, there is a shallow shallow depth of aquifer, no geological disaster vulnerability and low intensity TENORM radiation exposure. The results of the land-use evaluation for the Suban reclamation area are more suitable for the Residential Infrastructure area rather than for Productive Research area.
THE CAPABILITY OF SEDATIVE EFFECT FROM CELERY (Apium graveolens L.) FRACTION TO MALE MICE Kusuma, Shinta; Setiawan, Arum; Salni, Salni
BIOVALENTIA: Biological Research Journal Vol. 4 No. 2 (2018)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.343 KB) | DOI: 10.24233/BIOV.4.2.2018.111

Abstract

Celery fraction research (Apium graveolens L.) was carried out to determine the ability of the sedation effect of celery fraction compared to celery extracts which have been known to have the ability to effect the previous sedation. This study aims to find out which fraction has the best sedation effect. This study was an experimental study with a Completely Randomized Design (CRD) consisting of 5 treatments and 5 replications. Test animals divided into 5 treatment groups namely negative control group (CMC Na 1%), celery extract group 200mg /kg and 3 treatment groups n-hexane fraction, ethyl acetate, and methanol water fraction with a dose of 200 mg /kg . The sedation effect test was carried out using the Traction Test and Fireplace Testmethods. Quantitative data observed were the length of time the mice fell and the length of time the mice went out of the heated tube/glass. The results of the analysis showed that the celery fraction had a better sedation effect than the extract, and the methanol water fraction 200 mg/kg was the most effective fraction in causing sedation effects.
ANTIOXIDANT ACTIVITY of THE SECONDARY METABOLITES PRODUCED by ENDOPHYTIC FUNGI ISOLATED from JERUJU (Acanthus ilicifolius L.) PLANT Putri, Amanda Rahmaniah; Salni, Salni; Widjajanti, Harry
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.374 KB) | DOI: 10.24233/BIOV.5.2.2019.153

Abstract

Twelve endophytic fungi were isolated from Jeruju plant. Three of which have the best antioxidant activity, namely Culvularia lunata DJS1, Diaporthe phaseolorum DJS2, and Colletotrichum siamense BJS4. The fungi can produce secondary metabolites that have the potential as antioxidant compounds. This study aims to isolate the antioxidant compound using a chromatography column and test the antioxidant activity using DPPH method. Isolation of secondary metabolites produced 40 eluates. The IC50 value of DJS111 and BJS42 eluates were 10.20 µg/ml and 20.89 µg/ml, which is an antioxidant compound with very strong activity. The IC50 value of DJS24 eluate was 54.45 µg/ml, which is strong antioxidant activity. DJS111 eluate has antioxidant activity that approaches to ascorbic acid as a standart antioxidant compound (7.76µg/ml). From TLC, DJS111 contains flavonoid compound and DJS24 and BJS42 contain terpenoid compound.